Rroxscaffold_7G00179430

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007046
Physical Location & Seq
Reverse (-)
19017439 .. 19022394
4956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179430.1

Sequence Viewer

Length: 1818 bp
ATGTTACCTGACTTACAGTTTGCTTGGAGGAACTTGTCAAGTGGGGTTTGGATTAGAACTCTAGGAATTGGGTTTTTTGGTGAAGTTTTGGGATTTGAAGAAGTGGGTATAGAGGTATTACTAGCTTGGTTTGTGAAGAAAGATGAAAGCTTGGTTGAGGTGTGGGAATTTGAAGATGTGGGTCTTTCTGGGTTTGTGGATAAAGGTGGTAACTTGCATTTTGAAGCGGCAATTCTTGCGGAGGATGACATGGTAGTAGCAGCTACAAGGACCAAACTCAACATGCAGACAGAAGAAAACAACGGCAAAACCACCTTCACACATATCCTATCAGCCGCTGCCATCGGCATCATCATTGCCGCGGCGGTGCATTATCGTCAACGAAAGACAAAGTATGAGAAGATCATCCCGCGCTTAAGGGTCTCGGACTCCAGCCGCGTTCATAAGATTGAAAGGTTCCCTCATTATGTAGCTAGGCAGCTGGGATTTAAAGATAGAAGAGAATGTCCACACCTCTGCAAATTGGCCACCGAATACATGAGAAAATGCGACGGGTTTGAGGATGATATCTACAGTTTCTTTTCTAGTGAACCGGATGCGGATTCGCTGTTTGTGAAGCTTCTGGAGGAGTTTGAGAGATGCATCCTCAGTTATTTCGCATTTCATTGGAACGAAGCTGATCTGATGATTAGTCAGGTCTTGAGTTGTGATACTGAGCCGAGGAAGAAGCTGAAGAACATTGTCATGGCAGCGACCAGGGAACAGAGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTGGAGGAGATGAAAGCAATGGGACTGGCCACAGCGGATGACTCAACATGTACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTCATGGGAGGTGGAATGGGAGCAGGGAAGAGCACTGTGCTAAAGGACATTCTCAAAGAGCCATTCTGGGCTGGAGCAGCAGGGAATGCAGTAGTGATTGAAGCAGATGCCTTCAAAGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAATCATCTACAGACGCAGCATCATCCCTTCTGGTCACAGCACTTAACGAAGGGCGAGATGTGATCATGGATGGCACACTCTCTTGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGCCGTTATCGCATGGGAGCTGGTTACAGGGTGAATGATGATGGAACTGTCACTGAAAACTATTGGGAACGAATTGAAGATGAAGAACCAGAACAAGTAGAAGGGGGAAGAAAGAGAAAACCATACAGGATAGAGCTTGTTGGAGTTGTGTGTGATGCTTATCTGGCTGTTATAAGAGGAATAAGGCGAGCTATCATGTGTAGAAGAGCTGTTCGAGTAAACGCACAATTGAAATCCCACAAGAGATTTTCAGAGGCATTTAAAACATATTGCCAACTAGTTGACAATGCTAGACTGTATAGCACTAATACGTTGGAAGGACCACCTAAGTTGATAGGATGGAAAGACAAAGACAAGACCTTGCTGGTTGATCCAGATGAAATTGATTGCTTAAAAAGGGTGGCAAGTTTGAATGACAGAGCGACTTCCATATACGAACTCTACAAAAACCCTAATCCAGCTTGTCAAAAGGGATCAGTTTGGAAGGACATTGTCCTGTCACCTTCAAGGTTAAACATTCAACAGGAGCTGAAGTACTCTATCCAGAAAGTAGAAAGGTTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

605

Amino Acids

68.65

Weight (kDa)

7.19

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 299 - 402 3.2e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1421
Acc65I GGTACC 1 cut(s) 1201
AccB1I GGYRCC 1 cut(s) 1201
AccII CGCG 3 cut(s) 362, 412, 438
AclI AACGTT 1 cut(s) 815
AclWI GGATC 2 cut(s) 1613, 1729
AcoI YGGCCR 3 cut(s) 525, 846, 1230
AcsI RAATTY 2 cut(s) 167, 787
AcuI CTGAAG 2 cut(s) 752, 1799
AfaI GTAC 4 cut(s) 871, 1110, 1203, 1784
AfiI CCNNNNNNNGG 1 cut(s) 925
AflII CTTAAG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 866
AhlI ACTAGT 1 cut(s) 1525
AjnI CCWGG 1 cut(s) 755
Alw21I GWGCWC 2 cut(s) 956, 1063
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 2 cut(s) 1613, 1729
AlwNI CAGNNNCTG 2 cut(s) 338, 1777
AoxI GGCC 3 cut(s) 525, 846, 1230
ApeKI GCWGC 6 cut(s) 260, 338, 478, 749, 998, 1130
ApoI RAATTY 2 cut(s) 167, 787
ArsI GACNNNNNNTTYG 3 cut(s) 34, 772, 804
Asp718I GGTACC 1 cut(s) 1201
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 270, 1568
AsuHPI GGTGA 5 cut(s) 92, 790, 889, 1291, 1740
AvaII GGWCC 2 cut(s) 270, 1568
BalI TGGCCA 3 cut(s) 527, 848, 1232
BanI GGYRCC 1 cut(s) 1201
BanII GRGCYC 1 cut(s) 1063
Bbv12I GWGCWC 2 cut(s) 956, 1063
BbvI GCAGC 6 cut(s) 272, 325, 490, 761, 1010, 1142
BccI CCATC 5 cut(s) 350, 874, 1178, 1283, 1581
BceAI ACGGC 2 cut(s) 319, 1236
BciT130I CCWGG 1 cut(s) 757
BclI TGATCA 1 cut(s) 1176
BcoDI GTCTC 1 cut(s) 427
BcuI ACTAGT 1 cut(s) 1525
BfaI CTAG 7 cut(s) 62, 122, 474, 585, 801, 1526, 1539
BfmI CTRYAG 2 cut(s) 571, 1122
BfrI CTTAAG 1 cut(s) 415
BmcAI AGTACT 1 cut(s) 1784
Bme1390I CCNGG 1 cut(s) 757
Bme18I GGWCC 2 cut(s) 270, 1568
BmgT120I GGNCC 2 cut(s) 270, 1568
BmiI GGNNCC 3 cut(s) 458, 885, 1203
BmrFI CCNGG 1 cut(s) 757
BmsI GCATC 8 cut(s) 357, 586, 629, 651, 1018, 1087, 1142, 1393
BpmI CTGGAG 5 cut(s) 415, 644, 842, 1014, 1054
BpuEI CTTGAG 1 cut(s) 721
BsaBI GATNNNNATC 1 cut(s) 1407
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 3 cut(s) 360, 719, 756
BsaWI WCCGGW 2 cut(s) 592, 886
BsaXI ACNNNNNCTCC 2 cut(s) 1766, 1796
Bsc4I CCNNNNNNNGG 1 cut(s) 925
Bse1I ACTGG 4 cut(s) 825, 849, 911, 1110
Bse3DI GCAATG 2 cut(s) 354, 843
Bse8I GATNNNNATC 1 cut(s) 1407
BseBI CCWGG 1 cut(s) 757
BseDI CCNNGG 3 cut(s) 360, 719, 756
BseJI GATNNNNATC 1 cut(s) 1407
BseLI CCNNNNNNNGG 1 cut(s) 925
BseMI GCAATG 2 cut(s) 354, 843
BseMII CTCAG 3 cut(s) 661, 705, 1078
BseNI ACTGG 4 cut(s) 825, 849, 911, 1110
BseRI GAGGAG 3 cut(s) 641, 839, 908
BseXI GCAGC 6 cut(s) 272, 325, 490, 761, 1010, 1142
BseYI CCCAGC 1 cut(s) 481
Bsh1236I CGCG 3 cut(s) 362, 412, 438
BshFI GGCC 3 cut(s) 527, 848, 1232
BshNI GGYRCC 1 cut(s) 1201
BsiHKAI GWGCWC 2 cut(s) 956, 1063
BsiSI CCGG 2 cut(s) 593, 887
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 1 cut(s) 925
BsmAI GTCTC 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 855
BsmI GAATGC 1 cut(s) 1012
BsnI GGCC 3 cut(s) 527, 848, 1232
Bso31I GGTCTC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 956, 1063
Bsp1407I TGTACA 1 cut(s) 869
Bsp143I GATC 5 cut(s) 402, 679, 1176, 1618, 1721
BspANI GGCC 3 cut(s) 527, 848, 1232
BspCNI CTCAG 3 cut(s) 660, 706, 1077
BspFNI CGCG 3 cut(s) 362, 412, 438
BspLI GGNNCC 3 cut(s) 458, 885, 1203
BspPI GGATC 2 cut(s) 1613, 1729
BspQI GCTCTTC 2 cut(s) 944, 1447
BspT107I GGYRCC 1 cut(s) 1201
BspTI CTTAAG 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 2 cut(s) 354, 843
BsrGI TGTACA 1 cut(s) 869
BsrI ACTGG 4 cut(s) 825, 849, 911, 1110
BssECI CCNNGG 3 cut(s) 360, 719, 756
BssMI GATC 5 cut(s) 402, 679, 1176, 1618, 1721
Bst2UI CCWGG 1 cut(s) 757
Bst4CI ACNGT 6 cut(s) 18, 575, 958, 1222, 1297, 1545
Bst6I CTCTTC 4 cut(s) 493, 926, 944, 1447
BstAFI CTTAAG 1 cut(s) 415
BstAPI GCANNNNNTGC 2 cut(s) 236, 1007
BstAUI TGTACA 1 cut(s) 869
BstC8I GCNNGC 1 cut(s) 1437
BstDEI CTNAG 4 cut(s) 647, 714, 1064, 1575
BstDSI CCRYGG 1 cut(s) 360
BstEII GGTNACC 1 cut(s) 778
BstFNI CGCG 3 cut(s) 362, 412, 438
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 5 cut(s) 405, 682, 1179, 1621, 1724
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 5 cut(s) 402, 679, 1176, 1618, 1721
BstMWI GCNNNNNNNGC 6 cut(s) 236, 890, 998, 1007, 1257, 1412
BstNI CCWGG 1 cut(s) 757
BstNSI RCATGY 3 cut(s) 286, 870, 1090
BstPI GGTNACC 1 cut(s) 778
BstSCI CCNGG 1 cut(s) 755
BstSFI CTRYAG 2 cut(s) 571, 1122
BstUI CGCG 3 cut(s) 362, 412, 438
BstV1I GCAGC 6 cut(s) 272, 325, 490, 761, 1010, 1142
BsuRI GGCC 3 cut(s) 527, 848, 1232
BtgI CCRYGG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 954, 1299
Cac8I GCNNGC 1 cut(s) 1437
CaiI CAGNNNCTG 2 cut(s) 338, 1777
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 2 cut(s) 270, 1568
Cfr42I CCGCGG 1 cut(s) 363
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 4 cut(s) 870, 1109, 1202, 1783
CspCI CAANNNNNGTGG 2 cut(s) 1560, 1595
CviQI GTAC 4 cut(s) 870, 1109, 1202, 1783
DdeI CTNAG 4 cut(s) 647, 714, 1064, 1575
DpnI GATC 5 cut(s) 404, 681, 1178, 1620, 1723
DpnII GATC 5 cut(s) 402, 679, 1176, 1618, 1721
DraI TTTAAA 2 cut(s) 490, 1510
EaeI YGGCCR 3 cut(s) 525, 846, 1230
Eam1104I CTCTTC 4 cut(s) 493, 926, 944, 1447
EarI CTCTTC 4 cut(s) 493, 926, 944, 1447
Ecl136II GAGCTC 1 cut(s) 1061
Eco24I GRGCYC 1 cut(s) 1063
Eco31I GGTCTC 1 cut(s) 427
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 270, 1568
Eco53kI GAGCTC 1 cut(s) 1061
Eco57I CTGAAG 2 cut(s) 752, 1799
Eco91I GGTNACC 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 1061
EcoO65I GGTNACC 1 cut(s) 778
EcoRII CCWGG 1 cut(s) 755
EcoRV GATATC 1 cut(s) 568
EcoT22I ATGCAT 1 cut(s) 644
EcoT38I GRGCYC 1 cut(s) 1063
FaqI GGGAC 1 cut(s) 855
FauI CCCGC 1 cut(s) 417
FbaI TGATCA 1 cut(s) 1176
FriOI GRGCYC 1 cut(s) 1063
FspBI CTAG 7 cut(s) 62, 122, 474, 585, 801, 1526, 1539
GlaI GCGC 1 cut(s) 413
GsaI CCCAGC 1 cut(s) 485
GsuI CTGGAG 5 cut(s) 415, 644, 842, 1014, 1054
HaeIII GGCC 3 cut(s) 527, 848, 1232
HapII CCGG 2 cut(s) 593, 887
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 1 cut(s) 414
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 2 cut(s) 380, 1531
HindII GTYRAC 2 cut(s) 380, 1531
HindIII AAGCTT 2 cut(s) 148, 617
HinfI GANTC 4 cut(s) 428, 602, 860, 1040
HpaII CCGG 2 cut(s) 593, 887
HphI GGTGA 5 cut(s) 92, 790, 889, 1291, 1740
Hpy166II GTNNAC 7 cut(s) 380, 509, 590, 1111, 1243, 1468, 1531
Hpy188I TCNGA 5 cut(s) 427, 684, 1045, 1218, 1501
Hpy188III TCNNGA 6 cut(s) 623, 700, 1071, 1082, 1622, 1792
Hpy8I GTNNAC 7 cut(s) 380, 509, 590, 1111, 1243, 1468, 1531
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 9 cut(s) 325, 787, 1042, 1151, 1157, 1343, 1559, 1726, 1761
HpyCH4III ACNGT 6 cut(s) 18, 575, 958, 1222, 1297, 1545
HpyCH4IV ACGT 2 cut(s) 815, 1559
HpyCH4V TGCA 6 cut(s) 217, 286, 370, 519, 642, 1010
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 890, 998, 1007, 1257, 1412
HpyF3I CTNAG 4 cut(s) 647, 714, 1064, 1575
HpySE526I ACGT 2 cut(s) 815, 1559
HspAI GCGC 1 cut(s) 412
KpnI GGTACC 1 cut(s) 1205
Ksp22I TGATCA 1 cut(s) 1176
KspI CCGCGG 1 cut(s) 363
Kzo9I GATC 5 cut(s) 402, 679, 1176, 1618, 1721
LguI GCTCTTC 2 cut(s) 944, 1447
LmnI GCTCC 6 cut(s) 889, 941, 995, 1073, 1265, 1774
Lsp1109I GCAGC 6 cut(s) 272, 325, 490, 761, 1010, 1142
LweI GCATC 8 cut(s) 357, 586, 629, 651, 1018, 1087, 1142, 1393
MaeI CTAG 7 cut(s) 62, 122, 474, 585, 801, 1526, 1539
MaeII ACGT 2 cut(s) 815, 1559
MaeIII GTNAC 9 cut(s) 3, 209, 778, 816, 1147, 1222, 1271, 1297, 1746
MalI GATC 5 cut(s) 404, 681, 1178, 1620, 1723
MboI GATC 5 cut(s) 402, 679, 1176, 1618, 1721
MfeI CAATTG 1 cut(s) 1475
MhlI GDGCHC 2 cut(s) 956, 1063
MlsI TGGCCA 3 cut(s) 527, 848, 1232
MluCI AATT 8 cut(s) 66, 167, 231, 521, 787, 1320, 1475, 1629
MluNI TGGCCA 3 cut(s) 527, 848, 1232
MlyI GAGTC 2 cut(s) 422, 854
MmeI TCCRAC 2 cut(s) 1369, 1542
Mox20I TGGCCA 3 cut(s) 527, 848, 1232
Mph1103I ATGCAT 1 cut(s) 644
MscI TGGCCA 3 cut(s) 527, 848, 1232
MseI TTAA 6 cut(s) 416, 489, 1158, 1509, 1640, 1760
MslI CAYNNNNRTG 1 cut(s) 743
Msp20I TGGCCA 3 cut(s) 527, 848, 1232
MspA1I CMGCKG 5 cut(s) 338, 362, 481, 854, 1100
MspCI CTTAAG 1 cut(s) 415
MspI CCGG 2 cut(s) 593, 887
MspR9I CCNGG 1 cut(s) 757
MunI CAATTG 1 cut(s) 1475
Mva1269I GAATGC 1 cut(s) 1012
MvaI CCWGG 1 cut(s) 757
MvnI CGCG 3 cut(s) 362, 412, 438
MwoI GCNNNNNNNGC 6 cut(s) 236, 890, 998, 1007, 1257, 1412
NdeII GATC 5 cut(s) 402, 679, 1176, 1618, 1721
NlaIV GGNNCC 3 cut(s) 458, 885, 1203
NmeAIII GCCGAG 1 cut(s) 744
NmuCI GTSAC 4 cut(s) 778, 1147, 1297, 1746
NsiI ATGCAT 1 cut(s) 644
NspI RCATGY 3 cut(s) 286, 870, 1090
PciI ACATGT 1 cut(s) 866
PciSI GCTCTTC 2 cut(s) 944, 1447
PctI GAATGC 1 cut(s) 1012
PfeI GAWTC 2 cut(s) 602, 1040
PflFI GACNNNGTC 1 cut(s) 1739
PleI GAGTC 2 cut(s) 422, 854
PpsI GAGTC 2 cut(s) 422, 854
PscI ACATGT 1 cut(s) 866
PsiI TTATAA 1 cut(s) 1421
Psp124BI GAGCTC 1 cut(s) 1063
Psp1406I AACGTT 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 755
PspEI GGTNACC 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 481
PspGI CCWGG 1 cut(s) 755
PspN4I GGNNCC 3 cut(s) 458, 885, 1203
PspPI GGNCC 2 cut(s) 270, 1568
PstNI CAGNNNCTG 2 cut(s) 338, 1777
PsyI GACNNNGTC 1 cut(s) 1739
PvuII CAGCTG 2 cut(s) 481, 1100
RsaI GTAC 4 cut(s) 871, 1110, 1203, 1784
RsaNI GTAC 4 cut(s) 870, 1109, 1202, 1783
RseI CAYNNNNRTG 1 cut(s) 743
SacI GAGCTC 1 cut(s) 1063
SacII CCGCGG 1 cut(s) 363
SapI GCTCTTC 2 cut(s) 944, 1447
SaqAI TTAA 6 cut(s) 416, 489, 1158, 1509, 1640, 1760
Sau3AI GATC 5 cut(s) 402, 679, 1176, 1618, 1721
Sau96I GGNCC 2 cut(s) 270, 1568
ScaI AGTACT 1 cut(s) 1784
SchI GAGTC 2 cut(s) 422, 854
ScrFI CCNGG 1 cut(s) 757
SduI GDGCHC 2 cut(s) 956, 1063
SfaNI GCATC 8 cut(s) 357, 586, 629, 651, 1018, 1087, 1142, 1393
SfcI CTRYAG 2 cut(s) 571, 1122
Sfr303I CCGCGG 1 cut(s) 363
SgrBI CCGCGG 1 cut(s) 363
SinI GGWCC 2 cut(s) 270, 1568
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 2 cut(s) 415, 700
SmoI CTYRAG 2 cut(s) 415, 700
SpeI ACTAGT 1 cut(s) 1525
Sse9I AATT 8 cut(s) 66, 167, 231, 521, 787, 1320, 1475, 1629
SspMI CTAG 7 cut(s) 62, 122, 474, 585, 801, 1526, 1539
SstI GAGCTC 1 cut(s) 1063
StyD4I CCNGG 1 cut(s) 755
TaaI ACNGT 6 cut(s) 18, 575, 958, 1222, 1297, 1545
TaiI ACGT 2 cut(s) 818, 1562
TaqI TCGA 1 cut(s) 1462
TasI AATT 8 cut(s) 66, 167, 231, 521, 787, 1320, 1475, 1629
TatI WGTACW 2 cut(s) 869, 1782
TauI GCSGC 6 cut(s) 230, 338, 362, 365, 438, 1251
TfiI GAWTC 2 cut(s) 602, 1040
Tru1I TTAA 6 cut(s) 416, 489, 1158, 1509, 1640, 1760
Tru9I TTAA 6 cut(s) 416, 489, 1158, 1509, 1640, 1760
TscAI CASTG 2 cut(s) 961, 1306
TseFI GTSAC 4 cut(s) 778, 1147, 1297, 1746
TseI GCWGC 6 cut(s) 260, 338, 478, 749, 998, 1130
Tsp45I GTSAC 4 cut(s) 778, 1147, 1297, 1746
TspDTI ATGAA 7 cut(s) 159, 431, 653, 845, 913, 1344, 1641
TspRI CASTG 2 cut(s) 961, 1306
Tth111I GACNNNGTC 1 cut(s) 1739
Vha464I CTTAAG 1 cut(s) 415
VpaK11BI GGWCC 2 cut(s) 270, 1568
XapI RAATTY 2 cut(s) 167, 787
XceI RCATGY 3 cut(s) 286, 870, 1090
XspI CTAG 7 cut(s) 62, 122, 474, 585, 801, 1526, 1539
ZrmI AGTACT 1 cut(s) 1784
Zsp2I ATGCAT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.