AT2G30630

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007046
Physical Location & Seq
Reverse (-)
13046662 .. 13049219
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30630.2

Sequence Viewer

Length: 1596 bp
ATGTCTCTTGATGATCCCCAAAGCAAATCCAGATCAATGTGTGTTCCAGAGTTTAACAGCAACAACAACACGACGACCTTCGCACAGATTTTAGTAATCTCATCCATTGGCTTACTCTTAGCCCTTGCACTGCACTTCCGTCTAAGGAAGCTACGTCACTCCAAGAACATCCCTCGTCTCAGATCGTCTCAAAAACACAAAGGCCATGAGAAGCTTGAGAGATTCTCTCACTATGTTGTGCGGCAGATGGGATTCAAGGACAGGAGAGAATGTCCTCATCTCTGCAAATTAGCTAATGAGTACATAAGAAAATCAGGCAGCTGTGAGGAAGACATATACAGCTTCTTCTCTGAAGAGCCTGGTGCTGATTCTCTCTTCATTAAGCTCGTTGAAGAGTTTGAGAGATGTATTCTTAGTTACTTTGCTTACCACTGGAGCCATGCGGATCTCATGATCAGTCAGATACTCAGTGCCGATGTAGAGCCTAAGAAGAAGCTAAAGCACATTGTCATGGCGGCTACAAGGGAACAGAGGTTTGAGAGGGTGACTAAAAATCTAAAAGTGGCAAGAGTGTTTAACACATTGGTAGAGGAAATGAAAGCAATGGGGATCGCATCTGTTGATGACTCAGAGTGTACAGAAGTTATGGCTCCAGTTGCACACAAGGACCGAAGCCCGGTTCTACTTCTTATGGGAGGTGGTATGGGTGCAGGAAAGAGCACTGTGCTTAAAGACATTCTCAAAGAACCGTTTTGGGCAGGAGCTGATGCTGTGGTGATTGAAGCCGATGCTTTTAAAGAATCTGATGTGATATACAGAGCTTTAAGCGCCAGAGGTCATGTTGATATGATCAAGACTGCTGAATTCGTTCACCAATCATCAACAGACGCAGCATCATCGCTGCTCGTTACAGCTCTCAACGAAGGGCGAGACGTGATAATGGATGGAACACTCTCTTGGGTACCATTTGTGGTTCAGACCATAACAATGGCGAGGAATGTGCATCGCCATCGTTACAGAATGGGAGCTGGCTACAAGGTTGGTGAGAATGGAGATGTTATAGAGAACTATTGGGAACGCATAGGCGAAAGGCAACAGCTTCAAGAAGATGGAAGGGAGAGAAAGCCATACAGGATAGAGTTAGTTGGAGTTGTATGTGATGCATATTTAGCAGTGATTCGAGGAATTAGGAGAGCTATAATGTGTAGAAGAGCAGTTAGAGTGAGGTCTCAGCTGAGATCTCACAAGAGGTTTGCGAATGCGTTTCTTACCTATTGCAACTTAGTTGACAATGCTAGACTTTACTGCACCAATGCTCTTGAAGGTTCTCCAAAGCTGATAGGGTGGAAAGAGAAGGAAAAGACATTGCTAGTGGATCCAGAGGAGATAGACTGTTTGAAGAATGTAGGGAGGTTAAACGAGAATGCAGATTCTATTTACGAGCTATACAGAAATCCAAATCCTGCTTGTGAAGCTGGATCAATATGGAAAGACATTGTTCTTTCTCCTTCTAGGTTCAACATTCAACAAGAGCTCAAATACTCAATTCAGAAAGTGGAAAGATTCAAACAGTATCTCCAAGAAACACCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

531

Amino Acids

60.73

Weight (kDa)

8.95

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 242 - 342 3.1e-13 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 961
AciI CCGC 3 cut(s) 241, 443, 515
AclWI GGATC 6 cut(s) 8, 453, 617, 1370, 1383, 1486
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 3 cut(s) 302, 637, 963
AfiI CCNNNNNNNGG 1 cut(s) 676
AgsI TTSAA 9 cut(s) 256, 392, 782, 1103, 1322, 1399, 1519, 1526, 1567
AjiI CACGTC 1 cut(s) 934
AjnI CCWGG 1 cut(s) 358
AjuI GAANNNNNNNTTGG 2 cut(s) 940, 972
Alw21I GWGCWC 2 cut(s) 722, 1536
Alw26I GTCTC 5 cut(s) 9, 182, 192, 924, 1233
AlwI GGATC 6 cut(s) 8, 453, 617, 1370, 1383, 1486
AlwNI CAGNNNCTG 1 cut(s) 764
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 318, 890, 901
ApoI RAATTY 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 867
Asp718I GGTACC 1 cut(s) 961
AspLEI GCGC 1 cut(s) 830
AspS9I GGNCC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 4 cut(s) 556, 787, 863, 1055
AvaII GGWCC 1 cut(s) 667
BamHI GGATCC 1 cut(s) 1375
BanI GGYRCC 1 cut(s) 961
BanII GRGCYC 1 cut(s) 1536
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 722, 1536
BbvI GCAGC 3 cut(s) 330, 888, 902
BccI CCATC 4 cut(s) 241, 938, 1017, 1103
BcgI CGANNNNNNTGC 6 cut(s) 879, 913, 982, 992, 1016, 1026
BciT130I CCWGG 1 cut(s) 360
BclI TGATCA 2 cut(s) 453, 849
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 5 cut(s) 9, 182, 192, 924, 1233
BfaI CTAG 3 cut(s) 1296, 1370, 1512
BfoI RGCGCY 1 cut(s) 831
BglII AGATCT 1 cut(s) 1238
BisI GCNGC 5 cut(s) 242, 319, 516, 891, 902
BlsI GCNGC 5 cut(s) 243, 320, 517, 892, 903
Bme1390I CCNGG 2 cut(s) 360, 677
Bme18I GGWCC 1 cut(s) 667
BmgBI CACGTC 1 cut(s) 934
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 4 cut(s) 437, 651, 963, 1377
BmrFI CCNGG 2 cut(s) 360, 677
BmsI GCATC 6 cut(s) 623, 757, 778, 902, 1012, 1150
BpiI GAAGAC 1 cut(s) 336
BplI GAGNNNNNCTC 4 cut(s) 209, 241, 211, 243
BpmI CTGGAG 2 cut(s) 454, 636
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 677
BsaI GGTCTC 1 cut(s) 1233
BsaXI ACNNNNNCTCC 4 cut(s) 256, 286, 1560, 1590
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 437, 653
Bse3DI GCAATG 2 cut(s) 609, 1364
BseBI CCWGG 1 cut(s) 360
BseGI GGATG 3 cut(s) 101, 168, 949
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 2 cut(s) 609, 1364
BseMII CTCAG 5 cut(s) 193, 481, 642, 1226, 1244
BseNI ACTGG 2 cut(s) 437, 653
BseRI GAGGAG 1 cut(s) 1397
BseXI GCAGC 3 cut(s) 330, 888, 902
BsgI GTGCAG 3 cut(s) 116, 729, 1291
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 961
BsiHKAI GWGCWC 2 cut(s) 722, 1536
BsiSI CCGG 1 cut(s) 677
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 5 cut(s) 9, 182, 192, 924, 1233
BsmBI CGTCTC 3 cut(s) 182, 192, 924
BsmI GAATGC 2 cut(s) 1264, 1429
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 1233
Bsp1286I GDGCHC 2 cut(s) 722, 1536
Bsp1407I TGTACA 1 cut(s) 635
BspACI CCGC 3 cut(s) 241, 443, 515
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 5 cut(s) 192, 480, 641, 1227, 1243
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 4 cut(s) 437, 651, 963, 1377
BspPI GGATC 6 cut(s) 8, 453, 617, 1370, 1383, 1486
BspQI GCTCTTC 2 cut(s) 348, 1204
BspT107I GGYRCC 1 cut(s) 961
BspTNI GGTCTC 1 cut(s) 1233
BsrDI GCAATG 2 cut(s) 609, 1364
BsrGI TGTACA 1 cut(s) 635
BsrI ACTGG 2 cut(s) 437, 653
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 4 cut(s) 724, 750, 1394, 1572
Bst6I CTCTTC 4 cut(s) 348, 380, 387, 1204
BstAUI TGTACA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 1030
BstF5I GGATG 3 cut(s) 101, 168, 949
BstH2I RGCGCY 1 cut(s) 831
BstHHI GCGC 1 cut(s) 830
BstMAI GTCTC 5 cut(s) 9, 182, 192, 924, 1233
BstMWI GCNNNNNNNGC 4 cut(s) 656, 827, 1169, 1472
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 358, 675
BstV1I GCAGC 3 cut(s) 330, 888, 902
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 3 cut(s) 445, 1238, 1375
BstXI CCANNNNNNTGG 1 cut(s) 988
BstYI RGATCY 3 cut(s) 445, 1238, 1375
BsuRI GGCC 1 cut(s) 204
BtgZI GCGATG 2 cut(s) 882, 989
BtrI CACGTC 1 cut(s) 934
BtsCI GGATG 3 cut(s) 101, 168, 949
BtsI GCAGTG 2 cut(s) 128, 1179
BtsIMutI CAGTG 5 cut(s) 128, 430, 475, 720, 1179
Cac8I GCNNGC 1 cut(s) 1030
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 830
Cfr13I GGNCC 1 cut(s) 667
CseI GACGC 1 cut(s) 896
Csp6I GTAC 3 cut(s) 301, 636, 962
CviAII CATG 5 cut(s) 206, 440, 451, 511, 839
CviQI GTAC 3 cut(s) 301, 636, 962
DraI TTTAAA 1 cut(s) 796
Eam1104I CTCTTC 4 cut(s) 348, 380, 387, 1204
EarI CTCTTC 4 cut(s) 348, 380, 387, 1204
Ecl136II GAGCTC 1 cut(s) 1534
Eco24I GRGCYC 1 cut(s) 1536
Eco31I GGTCTC 1 cut(s) 1233
Eco47I GGWCC 1 cut(s) 667
Eco53kI GAGCTC 1 cut(s) 1534
Eco57I CTGAAG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 1534
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 1165
EcoT38I GRGCYC 1 cut(s) 1536
Esp3I CGTCTC 3 cut(s) 182, 192, 924
FaeI CATG 5 cut(s) 209, 443, 454, 514, 842
FatI CATG 5 cut(s) 205, 439, 450, 510, 838
FbaI TGATCA 2 cut(s) 453, 849
Fnu4HI GCNGC 5 cut(s) 242, 319, 516, 891, 902
FokI GGATG 3 cut(s) 88, 155, 956
FriOI GRGCYC 1 cut(s) 1536
Fsp4HI GCNGC 5 cut(s) 242, 319, 516, 891, 902
FspBI CTAG 3 cut(s) 1296, 1370, 1512
GlaI GCGC 1 cut(s) 829
GluI GCNGC 5 cut(s) 242, 319, 516, 891, 902
GsuI CTGGAG 2 cut(s) 454, 636
HaeII RGCGCY 1 cut(s) 831
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 677
HgaI GACGC 1 cut(s) 896
HhaI GCGC 1 cut(s) 830
Hin1II CATG 5 cut(s) 209, 443, 454, 514, 842
Hin6I GCGC 1 cut(s) 828
HinP1I GCGC 1 cut(s) 828
HincII GTYRAC 1 cut(s) 1288
HindII GTYRAC 1 cut(s) 1288
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 8 cut(s) 222, 252, 368, 626, 800, 1177, 1430, 1563
HpaII CCGG 1 cut(s) 677
HphI GGTGA 4 cut(s) 556, 787, 863, 1055
Hpy166II GTNNAC 3 cut(s) 636, 871, 1288
Hpy188I TCNGA 7 cut(s) 182, 352, 462, 631, 805, 978, 1551
Hpy188III TCNNGA 8 cut(s) 8, 30, 47, 451, 853, 1103, 1319, 1379
Hpy8I GTNNAC 3 cut(s) 636, 871, 1288
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 6 cut(s) 88, 917, 1107, 1316, 1348, 1518
HpyCH4III ACNGT 4 cut(s) 724, 750, 1394, 1572
HpyCH4IV ACGT 2 cut(s) 154, 933
HpyF10VI GCNNNNNNNGC 4 cut(s) 656, 827, 1169, 1472
HpySE526I ACGT 2 cut(s) 154, 933
Hsp92II CATG 5 cut(s) 209, 443, 454, 514, 842
HspAI GCGC 1 cut(s) 828
KpnI GGTACC 1 cut(s) 965
Ksp22I TGATCA 2 cut(s) 453, 849
LguI GCTCTTC 2 cut(s) 348, 1204
LmnI GCTCC 4 cut(s) 435, 655, 761, 1025
Lsp1109I GCAGC 3 cut(s) 330, 888, 902
LweI GCATC 6 cut(s) 623, 757, 778, 902, 1012, 1150
MaeI CTAG 3 cut(s) 1296, 1370, 1512
MaeII ACGT 2 cut(s) 154, 933
MaeIII GTNAC 5 cut(s) 155, 416, 544, 907, 1013
MboII GAAGA 9 cut(s) 337, 341, 365, 367, 404, 502, 1118, 1221, 1411
MflI RGATCY 3 cut(s) 445, 1238, 1375
MhlI GDGCHC 2 cut(s) 722, 1536
MluCI AATT 4 cut(s) 287, 863, 1185, 1545
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 1126
Mph1103I ATGCAT 1 cut(s) 1165
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 7 cut(s) 54, 381, 576, 729, 795, 824, 1415
MslI CAYNNNNRTG 3 cut(s) 509, 986, 1591
MspA1I CMGCKG 2 cut(s) 321, 1234
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 2 cut(s) 360, 677
Mva1269I GAATGC 2 cut(s) 1264, 1429
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 656, 827, 1169, 1472
NciI CCSGG 1 cut(s) 677
NlaIII CATG 5 cut(s) 209, 443, 454, 514, 842
NlaIV GGNNCC 4 cut(s) 437, 651, 963, 1377
NmuCI GTSAC 2 cut(s) 155, 544
NsiI ATGCAT 1 cut(s) 1165
PagI TCATGA 1 cut(s) 450
PciSI GCTCTTC 2 cut(s) 348, 1204
PctI GAATGC 2 cut(s) 1264, 1429
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 7 cut(s) 222, 252, 368, 800, 1177, 1430, 1563
PkrI GCNGC 5 cut(s) 243, 320, 517, 892, 903
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 1536
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 4 cut(s) 437, 651, 963, 1377
PspPI GGNCC 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 3 cut(s) 445, 1238, 1375
PvuII CAGCTG 2 cut(s) 321, 1234
RsaI GTAC 3 cut(s) 302, 637, 963
RsaNI GTAC 3 cut(s) 301, 636, 962
RseI CAYNNNNRTG 3 cut(s) 509, 986, 1591
SacI GAGCTC 1 cut(s) 1536
SapI GCTCTTC 2 cut(s) 348, 1204
SaqAI TTAA 7 cut(s) 54, 381, 576, 729, 795, 824, 1415
SatI GCNGC 5 cut(s) 242, 319, 516, 891, 902
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 360, 677
SduI GDGCHC 2 cut(s) 722, 1536
SfaNI GCATC 6 cut(s) 623, 757, 778, 902, 1012, 1150
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 3 cut(s) 509, 986, 1591
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 4 cut(s) 287, 863, 1185, 1545
SsiI CCGC 3 cut(s) 241, 443, 515
SspMI CTAG 3 cut(s) 1296, 1370, 1512
SstI GAGCTC 1 cut(s) 1536
StyD4I CCNGG 2 cut(s) 358, 675
TaaI ACNGT 4 cut(s) 724, 750, 1394, 1572
TaiI ACGT 2 cut(s) 157, 936
TaqI TCGA 1 cut(s) 1180
TaqII GACCGA 1 cut(s) 684
TasI AATT 4 cut(s) 287, 863, 1185, 1545
TatI WGTACW 2 cut(s) 300, 635
TauI GCSGC 2 cut(s) 244, 518
TfiI GAWTC 7 cut(s) 222, 252, 368, 800, 1177, 1430, 1563
Tru1I TTAA 7 cut(s) 54, 381, 576, 729, 795, 824, 1415
Tru9I TTAA 7 cut(s) 54, 381, 576, 729, 795, 824, 1415
TscAI CASTG 5 cut(s) 135, 437, 475, 727, 1179
TseFI GTSAC 2 cut(s) 155, 544
TseI GCWGC 3 cut(s) 318, 890, 901
Tsp45I GTSAC 2 cut(s) 155, 544
TspDTI ATGAA 2 cut(s) 367, 611
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 5 cut(s) 135, 437, 475, 727, 1179
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 1 cut(s) 863
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 3 cut(s) 1296, 1370, 1512
Zsp2I ATGCAT 1 cut(s) 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.