RchiOBHm_Chr6g0289231

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007046
Physical Location & Seq
Forward (+)
52357745 .. 52361752
4008 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25946

Sequence Viewer

Length: 1599 bp
ATGGCTATATGTTTCCAGCCTTCATGCAAAAAGCTATGTAATTTGTCATTCCTCACCTCTCAATTTTCTTCAGAAGAAAACAACGGCAAAACCACCTTCGCACATATCCTATCAGCCGCTGCCATCGGCATCATCATTGCCGCGGCGGTGCATTATCGTCAACGAAAGACAAAGTATGAGAAGATCATCCCACGCTTAAGGGTCTCGGACTCCAGCCGCGTTCATAAGATTGAAAGGTTCCCTCATTATGTAGCTAGGCAGCTGGGATTTAAAGATAGAAGAGAATGTCCACACCTCTGCAAATTGGCCACCGAATACATGAGAAAATGCGACGGGTTTGAGGATGACATCTACAGTTTCTTTTCTAGTGAACCGGATGCGGACTCGCTGTTTGTGAAGCTTCTGGAGGAGTTTGAGAGATGCATCCTCAGTTATTTCGCATTTCATTGGAACGAAGCTGATCTGATGATTAGTCAGGTCTTGAGTTGTGATACTGAGCCGAGGAAGAAGCTCAAGAACATTGTCATGGCAGCGACCAGGGAACAGAGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTGGAGGAGATGAAAGCAATGGGACTGGCCACAGCGGATGACTCAACATGTACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTTATGGGAGGTGGAATGGGAGCAGGGAAGAGCACCGTGCTAAAGGACATTCTCAAAGAGCCATTCTGGGCAGGAGCAGCAGGGAATGCAGTAGTGATTGAAGCAGATGCCTTCAAAGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAATCATCTACAGACGCAGCATCATCCCTTCTGGTCACAGCACTTAACGAAGGGCGAGATGTGATCATGGATGGCACACTCTCTTGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGCCGTTATCGCATGGGAGCTGGTTACAGGGTGAATGATGATGGAACTGTCACTGAAAACTATTGGGAACGAATTGAAGATGAAGAACCAGAACAAGTAGAAGGGGGCAGAAAGAGAAAACCATACAGGATAGAGCTTGTTGGAGTTGTGTGTGATGCTTATCTGGCTGTTATAAGAGGAATAAGGCGAGCTATCATGTGTAGAAGAGCTGTTCGAGTAAACGCACAATTGAAATCCCACAAGAGATTTTCAGAGGCATTTAAAACATATTGCCAACTAGTTGACAATGCTAGACTGTATAGCACTAATACGTTGGAAGGACCACCTAAGTTGATAGGATGGAAAGACAAAGACAAGACCTTGCTGGTTGATCCAGATGAAATTGATTGCTTAAAAAGGGTGGCAAGTTTGAATGACAGAGCGACTTCCATATACGAACTCTACAAAAACCCTAATCCAGCTTGTCAAAAGGGATCAGTTTGGAAGGACATTGTCCTGTCGCCTTCAAGGTTAAACATTCAACAGGAGCTGAAGTACTCTATCCAGAAAGTAGAGAGATTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

532

Amino Acids

60.45

Weight (kDa)

8.89

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 226 - 329 2.6e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1202
Acc65I GGTACC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 982
AccII CGCG 2 cut(s) 143, 219
AciI CCGC 9 cut(s) 117, 141, 143, 146, 217, 380, 635, 1027, 1030
AclI AACGTT 1 cut(s) 596
AclWI GGATC 2 cut(s) 1394, 1510
AcoI YGGCCR 3 cut(s) 306, 627, 1011
AcsI RAATTY 1 cut(s) 568
AcuI CTGAAG 2 cut(s) 54, 1580
AfaI GTAC 4 cut(s) 652, 891, 984, 1565
AfiI CCNNNNNNNGG 1 cut(s) 706
AflII CTTAAG 1 cut(s) 196
AflIII ACRYGT 1 cut(s) 647
AhlI ACTAGT 1 cut(s) 1306
AjnI CCWGG 1 cut(s) 536
Alw21I GWGCWC 2 cut(s) 737, 844
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 2 cut(s) 1394, 1510
AlwNI CAGNNNCTG 2 cut(s) 119, 1558
AoxI GGCC 3 cut(s) 306, 627, 1011
ApeKI GCWGC 5 cut(s) 119, 259, 530, 779, 911
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 4 cut(s) 374, 406, 553, 585
Asp718I GGTACC 1 cut(s) 982
AspS9I GGNCC 1 cut(s) 1349
AsuHPI GGTGA 4 cut(s) 46, 571, 670, 1072
AvaII GGWCC 1 cut(s) 1349
BalI TGGCCA 3 cut(s) 308, 629, 1013
BanI GGYRCC 1 cut(s) 982
BanII GRGCYC 1 cut(s) 844
Bbv12I GWGCWC 2 cut(s) 737, 844
BbvI GCAGC 5 cut(s) 106, 271, 542, 791, 923
BccI CCATC 5 cut(s) 131, 655, 959, 1064, 1362
BceAI ACGGC 2 cut(s) 100, 1017
BciT130I CCWGG 1 cut(s) 538
BclI TGATCA 1 cut(s) 957
BcoDI GTCTC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 1306
BfaI CTAG 5 cut(s) 255, 366, 582, 1307, 1320
BfmI CTRYAG 2 cut(s) 352, 903
BfrI CTTAAG 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 1565
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 1 cut(s) 1349
BmgT120I GGNCC 1 cut(s) 1349
BmiI GGNNCC 3 cut(s) 239, 666, 984
BmrFI CCNGG 1 cut(s) 538
BmsI GCATC 8 cut(s) 138, 367, 410, 432, 799, 868, 923, 1174
BpmI CTGGAG 4 cut(s) 196, 425, 623, 835
BpuEI CTTGAG 2 cut(s) 497, 502
BsaBI GATNNNNATC 1 cut(s) 1188
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 141, 500, 537
BsaWI WCCGGW 2 cut(s) 373, 667
BsaXI ACNNNNNCTCC 2 cut(s) 1547, 1577
Bsc4I CCNNNNNNNGG 1 cut(s) 706
Bse1I ACTGG 4 cut(s) 606, 630, 692, 891
Bse3DI GCAATG 2 cut(s) 135, 624
Bse8I GATNNNNATC 1 cut(s) 1188
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 3 cut(s) 141, 500, 537
BseGI GGATG 9 cut(s) 186, 349, 382, 423, 643, 832, 917, 970, 1373
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 1 cut(s) 706
BseMI GCAATG 2 cut(s) 135, 624
BseMII CTCAG 3 cut(s) 442, 486, 859
BseNI ACTGG 4 cut(s) 606, 630, 692, 891
BseRI GAGGAG 3 cut(s) 422, 620, 689
BseXI GCAGC 5 cut(s) 106, 271, 542, 791, 923
BseYI CCCAGC 1 cut(s) 262
Bsh1236I CGCG 2 cut(s) 143, 219
BshFI GGCC 3 cut(s) 308, 629, 1013
BshNI GGYRCC 1 cut(s) 982
BsiHKAI GWGCWC 2 cut(s) 737, 844
BsiSI CCGG 2 cut(s) 374, 668
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 1 cut(s) 706
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 636
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 3 cut(s) 308, 629, 1013
Bso31I GGTCTC 1 cut(s) 208
Bsp1286I GDGCHC 2 cut(s) 737, 844
Bsp1407I TGTACA 1 cut(s) 650
Bsp143I GATC 5 cut(s) 183, 460, 957, 1399, 1502
BspACI CCGC 9 cut(s) 117, 141, 143, 146, 217, 380, 635, 1027, 1030
BspANI GGCC 3 cut(s) 308, 629, 1013
BspCNI CTCAG 3 cut(s) 441, 487, 858
BspFNI CGCG 2 cut(s) 143, 219
BspLI GGNNCC 3 cut(s) 239, 666, 984
BspPI GGATC 2 cut(s) 1394, 1510
BspQI GCTCTTC 2 cut(s) 725, 1228
BspT107I GGYRCC 1 cut(s) 982
BspTI CTTAAG 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 135, 624
BsrGI TGTACA 1 cut(s) 650
BsrI ACTGG 4 cut(s) 606, 630, 692, 891
BssECI CCNNGG 3 cut(s) 141, 500, 537
BssMI GATC 5 cut(s) 183, 460, 957, 1399, 1502
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 5 cut(s) 356, 739, 1003, 1078, 1326
Bst6I CTCTTC 3 cut(s) 274, 725, 1228
BstAFI CTTAAG 1 cut(s) 196
BstAPI GCANNNNNTGC 1 cut(s) 788
BstAUI TGTACA 1 cut(s) 650
BstC8I GCNNGC 1 cut(s) 1218
BstDEI CTNAG 4 cut(s) 428, 495, 845, 1356
BstDSI CCRYGG 1 cut(s) 141
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 9 cut(s) 186, 349, 382, 423, 643, 832, 917, 970, 1373
BstFNI CGCG 2 cut(s) 143, 219
BstKTI GATC 5 cut(s) 186, 463, 960, 1402, 1505
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 5 cut(s) 183, 460, 957, 1399, 1502
BstMWI GCNNNNNNNGC 5 cut(s) 671, 779, 788, 1038, 1193
BstNI CCWGG 1 cut(s) 538
BstNSI RCATGY 2 cut(s) 651, 871
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 536
BstSFI CTRYAG 2 cut(s) 352, 903
BstUI CGCG 2 cut(s) 143, 219
BstV1I GCAGC 5 cut(s) 106, 271, 542, 791, 923
BsuRI GGCC 3 cut(s) 308, 629, 1013
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 9 cut(s) 186, 349, 382, 423, 643, 832, 917, 970, 1373
BtsIMutI CAGTG 1 cut(s) 1080
Cac8I GCNNGC 1 cut(s) 1218
CaiI CAGNNNCTG 2 cut(s) 119, 1558
Cfr13I GGNCC 1 cut(s) 1349
Cfr42I CCGCGG 1 cut(s) 144
CseI GACGC 1 cut(s) 917
Csp6I GTAC 4 cut(s) 651, 890, 983, 1564
CspCI CAANNNNNGTGG 2 cut(s) 1341, 1376
CviAII CATG 9 cut(s) 24, 319, 526, 648, 677, 868, 961, 1042, 1225
CviQI GTAC 4 cut(s) 651, 890, 983, 1564
DdeI CTNAG 4 cut(s) 428, 495, 845, 1356
DpnI GATC 5 cut(s) 185, 462, 959, 1401, 1504
DpnII GATC 5 cut(s) 183, 460, 957, 1399, 1502
DraI TTTAAA 2 cut(s) 271, 1291
EaeI YGGCCR 3 cut(s) 306, 627, 1011
Eam1104I CTCTTC 3 cut(s) 274, 725, 1228
EarI CTCTTC 3 cut(s) 274, 725, 1228
Ecl136II GAGCTC 1 cut(s) 842
Eco24I GRGCYC 1 cut(s) 844
Eco31I GGTCTC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 1349
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 2 cut(s) 54, 1580
Eco91I GGTNACC 1 cut(s) 559
EcoICRI GAGCTC 1 cut(s) 842
EcoO65I GGTNACC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 536
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 844
FaeI CATG 9 cut(s) 27, 322, 529, 651, 680, 871, 964, 1045, 1228
FaqI GGGAC 1 cut(s) 636
FatI CATG 9 cut(s) 23, 318, 525, 647, 676, 867, 960, 1041, 1224
FbaI TGATCA 1 cut(s) 957
FokI GGATG 9 cut(s) 173, 356, 389, 410, 650, 839, 904, 977, 1380
FriOI GRGCYC 1 cut(s) 844
FspBI CTAG 5 cut(s) 255, 366, 582, 1307, 1320
GsaI CCCAGC 1 cut(s) 266
GsuI CTGGAG 4 cut(s) 196, 425, 623, 835
HaeIII GGCC 3 cut(s) 308, 629, 1013
HapII CCGG 2 cut(s) 374, 668
HgaI GACGC 1 cut(s) 917
Hin1II CATG 9 cut(s) 27, 322, 529, 651, 680, 871, 964, 1045, 1228
HincII GTYRAC 2 cut(s) 161, 1312
HindII GTYRAC 2 cut(s) 161, 1312
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 4 cut(s) 209, 383, 641, 821
HpaII CCGG 2 cut(s) 374, 668
HphI GGTGA 4 cut(s) 46, 571, 670, 1072
Hpy166II GTNNAC 7 cut(s) 161, 290, 371, 892, 1024, 1249, 1312
Hpy188I TCNGA 6 cut(s) 73, 208, 465, 826, 999, 1282
Hpy188III TCNNGA 7 cut(s) 404, 481, 514, 852, 863, 1403, 1573
Hpy8I GTNNAC 7 cut(s) 161, 290, 371, 892, 1024, 1249, 1312
Hpy99I CGWCG 1 cut(s) 335
HpyCH4III ACNGT 5 cut(s) 356, 739, 1003, 1078, 1326
HpyCH4IV ACGT 2 cut(s) 596, 1340
HpyCH4V TGCA 5 cut(s) 27, 151, 300, 423, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 671, 779, 788, 1038, 1193
HpyF3I CTNAG 4 cut(s) 428, 495, 845, 1356
HpySE526I ACGT 2 cut(s) 596, 1340
Hsp92II CATG 9 cut(s) 27, 322, 529, 651, 680, 871, 964, 1045, 1228
KpnI GGTACC 1 cut(s) 986
Ksp22I TGATCA 1 cut(s) 957
KspI CCGCGG 1 cut(s) 144
Kzo9I GATC 5 cut(s) 183, 460, 957, 1399, 1502
LguI GCTCTTC 2 cut(s) 725, 1228
LmnI GCTCC 6 cut(s) 670, 722, 776, 854, 1046, 1555
Lsp1109I GCAGC 5 cut(s) 106, 271, 542, 791, 923
LweI GCATC 8 cut(s) 138, 367, 410, 432, 799, 868, 923, 1174
MaeI CTAG 5 cut(s) 255, 366, 582, 1307, 1320
MaeII ACGT 2 cut(s) 596, 1340
MaeIII GTNAC 6 cut(s) 559, 597, 928, 1003, 1052, 1078
MalI GATC 5 cut(s) 185, 462, 959, 1401, 1504
MboI GATC 5 cut(s) 183, 460, 957, 1399, 1502
MboII GAAGA 9 cut(s) 60, 86, 193, 291, 517, 742, 1118, 1124, 1245
MfeI CAATTG 1 cut(s) 1256
MhlI GDGCHC 2 cut(s) 737, 844
MlsI TGGCCA 3 cut(s) 308, 629, 1013
MluCI AATT 7 cut(s) 40, 62, 302, 568, 1101, 1256, 1410
MluNI TGGCCA 3 cut(s) 308, 629, 1013
MlyI GAGTC 3 cut(s) 203, 377, 635
MmeI TCCRAC 2 cut(s) 1150, 1323
Mox20I TGGCCA 3 cut(s) 308, 629, 1013
Mph1103I ATGCAT 1 cut(s) 425
MscI TGGCCA 3 cut(s) 308, 629, 1013
MseI TTAA 6 cut(s) 197, 270, 939, 1290, 1421, 1541
MslI CAYNNNNRTG 1 cut(s) 524
Msp20I TGGCCA 3 cut(s) 308, 629, 1013
MspA1I CMGCKG 5 cut(s) 119, 143, 262, 635, 881
MspCI CTTAAG 1 cut(s) 196
MspI CCGG 2 cut(s) 374, 668
MspR9I CCNGG 1 cut(s) 538
MunI CAATTG 1 cut(s) 1256
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 1 cut(s) 538
MvnI CGCG 2 cut(s) 143, 219
MwoI GCNNNNNNNGC 5 cut(s) 671, 779, 788, 1038, 1193
NdeII GATC 5 cut(s) 183, 460, 957, 1399, 1502
NlaIII CATG 9 cut(s) 27, 322, 529, 651, 680, 871, 964, 1045, 1228
NlaIV GGNNCC 3 cut(s) 239, 666, 984
NmeAIII GCCGAG 1 cut(s) 525
NmuCI GTSAC 3 cut(s) 559, 928, 1078
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 2 cut(s) 651, 871
PciI ACATGT 1 cut(s) 647
PciSI GCTCTTC 2 cut(s) 725, 1228
PctI GAATGC 1 cut(s) 793
PfeI GAWTC 1 cut(s) 821
PflFI GACNNNGTC 1 cut(s) 1520
PleI GAGTC 3 cut(s) 203, 377, 635
PpsI GAGTC 3 cut(s) 203, 377, 635
PscI ACATGT 1 cut(s) 647
PsiI TTATAA 1 cut(s) 1202
Psp124BI GAGCTC 1 cut(s) 844
Psp1406I AACGTT 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 536
PspEI GGTNACC 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 262
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 3 cut(s) 239, 666, 984
PspPI GGNCC 1 cut(s) 1349
PstNI CAGNNNCTG 2 cut(s) 119, 1558
PsyI GACNNNGTC 1 cut(s) 1520
PvuII CAGCTG 2 cut(s) 262, 881
RsaI GTAC 4 cut(s) 652, 891, 984, 1565
RsaNI GTAC 4 cut(s) 651, 890, 983, 1564
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 844
SacII CCGCGG 1 cut(s) 144
SapI GCTCTTC 2 cut(s) 725, 1228
SaqAI TTAA 6 cut(s) 197, 270, 939, 1290, 1421, 1541
Sau3AI GATC 5 cut(s) 183, 460, 957, 1399, 1502
Sau96I GGNCC 1 cut(s) 1349
ScaI AGTACT 1 cut(s) 1565
SchI GAGTC 3 cut(s) 203, 377, 635
ScrFI CCNGG 1 cut(s) 538
SduI GDGCHC 2 cut(s) 737, 844
SfaNI GCATC 8 cut(s) 138, 367, 410, 432, 799, 868, 923, 1174
SfcI CTRYAG 2 cut(s) 352, 903
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SinI GGWCC 1 cut(s) 1349
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 3 cut(s) 196, 481, 512
SmoI CTYRAG 3 cut(s) 196, 481, 512
SpeI ACTAGT 1 cut(s) 1306
Sse9I AATT 7 cut(s) 40, 62, 302, 568, 1101, 1256, 1410
SsiI CCGC 9 cut(s) 117, 141, 143, 146, 217, 380, 635, 1027, 1030
SspMI CTAG 5 cut(s) 255, 366, 582, 1307, 1320
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 1 cut(s) 536
TaaI ACNGT 5 cut(s) 356, 739, 1003, 1078, 1326
TaiI ACGT 2 cut(s) 599, 1343
TaqI TCGA 1 cut(s) 1243
TasI AATT 7 cut(s) 40, 62, 302, 568, 1101, 1256, 1410
TatI WGTACW 2 cut(s) 650, 1563
TauI GCSGC 5 cut(s) 119, 143, 146, 219, 1032
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 6 cut(s) 197, 270, 939, 1290, 1421, 1541
Tru9I TTAA 6 cut(s) 197, 270, 939, 1290, 1421, 1541
TscAI CASTG 1 cut(s) 1087
TseFI GTSAC 3 cut(s) 559, 928, 1078
TseI GCWGC 5 cut(s) 119, 259, 530, 779, 911
Tsp45I GTSAC 3 cut(s) 559, 928, 1078
TspDTI ATGAA 6 cut(s) 12, 212, 434, 626, 1125, 1422
TspRI CASTG 1 cut(s) 1087
Tth111I GACNNNGTC 1 cut(s) 1520
Vha464I CTTAAG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 1349
XapI RAATTY 1 cut(s) 568
XceI RCATGY 2 cut(s) 651, 871
XspI CTAG 5 cut(s) 255, 366, 582, 1307, 1320
ZrmI AGTACT 1 cut(s) 1565
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.