Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001527.1

Orthogroup ID

OG0007046

Physical Location & Seq

Genomic Coordinates Forward (+)

53531 .. 56921

Gene Structure 3391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001527.1_g000012.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

atgttaccagacttacagtttgcttggaggaacttctcaagtggggtttggattggaactctaggaattcggttttttggtgaagttttgggatttgaagaagtgggtatagcggtattactactagcttggtttgtgaagaaagatgaaagcttggttgagctgtgggaatttgaagatgtgggtctttctgggttcgtggataaaggtggtaacttgcattttgaagcagcagttcttgcagaggatgacatggtagtagcacctacaaggaccaaactcagtttttgcctgtttatgccgctacaacttttccttttggtttcgccttcatgcaaaaagctatgtaatttgtcattcctcacctctcaattttcttcagaagaaaacaacggcaaaaccaccttcgcacatatcctatcagccgctgccatcggcatcatcattgccgcggcggtgcattatcgtcaacgaaagacaaagtatgagaagatcatcccacgcttaagggtctcggactccagccgcgttcataagatcgaaaggttccctcattatgtagctaggcagctgggatttaaagatagaagagaatgtccacacctctgcaaattggccaccgaatacatgagaaaatgcgacgggtttgaggatgatatctacagtttcttttctagtgaaccggatgcggactcgctgtttgtgaagcttctggaggagtttgagagatgcatcctcagttatttcgcatttcattggaacgaagctgatctgatgattagtcaggtcttgagttgtgatactgagccgaggaagaagctcaagaacatcgtcatggcagcgaccagggaacagagatttgaaagggtgaccaaaaatttgaaggtggctagagttttcacaacgttactggaggagatgaaagcaatgggactggccacagcggatgactcaacatgcacagaggtgatggctccggtggctcatgctgatagaagtccagtcctcctctttatgggaggtggaatgggagcagggaagagcaccgtgctaaaggacattctcaaagagccattctgggcaggagcagcagggaatgcagtagtgattgaagcagatgccttcaaagaatcggatgtcatatacagagctctcagctccagagggcatcacgacatgcttcaaacagctgaactggtacaccaatcatctacagacgcagcatcatcccttctggtcacagcacttaacgaagggagagatgtgatcatggatggcacactctcttgggtaccatttgttgttcagactgtaacaatggccagaaatgtccaccgccgccgttatcgcatgggagctggttacagggtgaatgacgatggaactgtcactgaaaactattgggaacgaattgaagatgaagaaccagaacaagtagaagggggcagaaagagaaaaccatacaggatagagcttgttggagttgtgtgtgatgcttatctggctgttataagaggaataaggcgagctatcatgtgtagaagagctgttcgagtaaacgcacaattgaaatcccacaagagattttcagaggcatttaaaacatattgccaactagttgacaatgctagactgtatagcactaatacgttggaaggaccacctaaggtacacctgctatcccttctcttagtttatcttcctttccggtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zeta_toxin

Protein Analysis

570

Amino Acids

64.5

Weight (kDa)

7.57

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06750 AT1G06750 AT1G06750 AT2G30630 AT2G30630 AT2G30630
fragaria_vesca	FvH4_2g22270
malus_domestica	MD05G1203900.v1.1 MD10G1191100.v1.1
prunus_persica	Prupe.8G230300_v2.0.a1 Prupe.8G230300_v2.0.a1
pyrus_communis	pycom05g19040 pycom10g16470
rosa_chinensis	RchiOBHm_Chr6g0289231
rosa_laevigata	RLG00000012319
rosa_multiflora	Rmu_sc0001527.1_g000012
rosa_roxburghii	Rroxscaffold_7G00179430
rosa_rugosa	Rorug06G0202100 Rorug06G0202200 Rorug06G0202200
rosa_samantha	Rh6AG313000 Rh6BG319900 Rh6CG326500 Rh6DG312500
rosa_wichuraiana	Rw6G027050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1511
AarI	CACCTGC	1 cut(s)	1683
Acc36I	ACCTGC	1 cut(s)	1683
Acc65I	GGTACC	1 cut(s)	1291
AccB1I	GGYRCC	1 cut(s)	1291
AccII	CGCG	2 cut(s)	452, 528
AclI	AACGTT	1 cut(s)	905
AcoI	YGGCCR	3 cut(s)	615, 936, 1320
AcsI	RAATTY	3 cut(s)	66, 170, 877
AcuI	CTGAAG	1 cut(s)	363
AfaI	GTAC	3 cut(s)	1200, 1293, 1671
AfiI	CCNNNNNNNGG	1 cut(s)	1015
AflII	CTTAAG	1 cut(s)	505
AhlI	ACTAGT	1 cut(s)	1615
AjnI	CCWGG	1 cut(s)	845
AleI	CACNNNNGTG	1 cut(s)	965
Alw21I	GWGCWC	2 cut(s)	1046, 1153
Alw26I	GTCTC	1 cut(s)	517
AlwNI	CAGNNNCTG	1 cut(s)	428
AoxI	GGCC	3 cut(s)	615, 936, 1320
ApeKI	GCWGC	6 cut(s)	230, 428, 568, 839, 1088, 1220
ApoI	RAATTY	3 cut(s)	66, 170, 877
ArsI	GACNNNNNNTTYG	5 cut(s)	34, 683, 715, 862, 894
Asp718I	GGTACC	1 cut(s)	1291
AspS9I	GGNCC	2 cut(s)	273, 1658
AsuHPI	GGTGA	5 cut(s)	92, 355, 880, 979, 1381
AvaII	GGWCC	2 cut(s)	273, 1658
AxyI	CCTNAGG	1 cut(s)	1665
BalI	TGGCCA	3 cut(s)	617, 938, 1322
BanI	GGYRCC	1 cut(s)	1291
BanII	GRGCYC	1 cut(s)	1153
Bbv12I	GWGCWC	2 cut(s)	1046, 1153
BbvI	GCAGC	6 cut(s)	242, 415, 580, 851, 1100, 1232
BccI	CCATC	4 cut(s)	440, 964, 1268, 1373
BceAI	ACGGC	2 cut(s)	409, 1326
BciT130I	CCWGG	1 cut(s)	847
BclI	TGATCA	1 cut(s)	1266
BcoDI	GTCTC	1 cut(s)	517
BcuI	ACTAGT	1 cut(s)	1615
BfaI	CTAG	7 cut(s)	62, 125, 564, 675, 891, 1616, 1629
BfmI	CTRYAG	2 cut(s)	661, 1212
BfrI	CTTAAG	1 cut(s)	505
BfuAI	ACCTGC	1 cut(s)	1683
Bme1390I	CCNGG	1 cut(s)	847
Bme18I	GGWCC	2 cut(s)	273, 1658
BmgT120I	GGNCC	2 cut(s)	273, 1658
BmiI	GGNNCC	3 cut(s)	548, 975, 1293
BmrFI	CCNGG	1 cut(s)	847
BmsI	GCATC	8 cut(s)	447, 676, 719, 741, 1108, 1177, 1232, 1483
BpmI	CTGGAG	4 cut(s)	505, 734, 932, 1144
BpuEI	CTTGAG	3 cut(s)	22, 806, 811
BsaBI	GATNNNNATC	1 cut(s)	1497
BsaI	GGTCTC	1 cut(s)	517
BsaJI	CCNNGG	3 cut(s)	450, 809, 846
BsaWI	WCCGGW	3 cut(s)	682, 976, 1707
Bsc4I	CCNNNNNNNGG	1 cut(s)	1015
Bse1I	ACTGG	4 cut(s)	915, 939, 1001, 1200
Bse21I	CCTNAGG	1 cut(s)	1665
Bse3DI	GCAATG	2 cut(s)	444, 933
Bse8I	GATNNNNATC	1 cut(s)	1497
BseBI	CCWGG	1 cut(s)	847
BseDI	CCNNGG	3 cut(s)	450, 809, 846
BseGI	GGATG	9 cut(s)	253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BseJI	GATNNNNATC	1 cut(s)	1497
BseLI	CCNNNNNNNGG	1 cut(s)	1015
BseMI	GCAATG	2 cut(s)	444, 933
BseMII	CTCAG	4 cut(s)	295, 751, 795, 1168
BseNI	ACTGG	4 cut(s)	915, 939, 1001, 1200
BseRI	GAGGAG	3 cut(s)	731, 929, 998
BseXI	GCAGC	6 cut(s)	242, 415, 580, 851, 1100, 1232
BseYI	CCCAGC	1 cut(s)	571
Bsh1236I	CGCG	2 cut(s)	452, 528
BshFI	GGCC	3 cut(s)	617, 938, 1322
BshNI	GGYRCC	1 cut(s)	1291
BsiHKAI	GWGCWC	2 cut(s)	1046, 1153
BsiSI	CCGG	3 cut(s)	683, 977, 1708
BslFI	GGGAC	1 cut(s)	945
BslI	CCNNNNNNNGG	1 cut(s)	1015
BsmAI	GTCTC	1 cut(s)	517
BsmFI	GGGAC	1 cut(s)	945
BsmI	GAATGC	1 cut(s)	1102
BsnI	GGCC	3 cut(s)	617, 938, 1322
Bso31I	GGTCTC	1 cut(s)	517
Bsp1286I	GDGCHC	2 cut(s)	1046, 1153
Bsp143I	GATC	4 cut(s)	492, 537, 769, 1266
BspANI	GGCC	3 cut(s)	617, 938, 1322
BspCNI	CTCAG	4 cut(s)	294, 750, 796, 1167
BspFNI	CGCG	2 cut(s)	452, 528
BspLI	GGNNCC	3 cut(s)	548, 975, 1293
BspMI	ACCTGC	1 cut(s)	1683
BspQI	GCTCTTC	2 cut(s)	1034, 1537
BspT107I	GGYRCC	1 cut(s)	1291
BspTI	CTTAAG	1 cut(s)	505
BspTNI	GGTCTC	1 cut(s)	517
BsrDI	GCAATG	2 cut(s)	444, 933
BsrI	ACTGG	4 cut(s)	915, 939, 1001, 1200
BssECI	CCNNGG	3 cut(s)	450, 809, 846
BssMI	GATC	4 cut(s)	492, 537, 769, 1266
Bst2UI	CCWGG	1 cut(s)	847
Bst4CI	ACNGT	6 cut(s)	18, 665, 1048, 1312, 1387, 1635
Bst6I	CTCTTC	3 cut(s)	583, 1034, 1537
BstAFI	CTTAAG	1 cut(s)	505
BstAPI	GCANNNNNTGC	2 cut(s)	239, 1097
BstC8I	GCNNGC	1 cut(s)	1527
BstDEI	CTNAG	6 cut(s)	281, 737, 804, 1154, 1665, 1690
BstDSI	CCRYGG	1 cut(s)	450
BstEII	GGTNACC	1 cut(s)	868
BstF5I	GGATG	9 cut(s)	253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BstFNI	CGCG	2 cut(s)	452, 528
BstKTI	GATC	4 cut(s)	495, 540, 772, 1269
BstMAI	GTCTC	1 cut(s)	517
BstMBI	GATC	4 cut(s)	492, 537, 769, 1266
BstMWI	GCNNNNNNNGC	6 cut(s)	239, 980, 1088, 1097, 1347, 1502
BstNI	CCWGG	1 cut(s)	847
BstNSI	RCATGY	2 cut(s)	960, 1180
BstPI	GGTNACC	1 cut(s)	868
BstSCI	CCNGG	1 cut(s)	845
BstSFI	CTRYAG	2 cut(s)	661, 1212
BstUI	CGCG	2 cut(s)	452, 528
BstV1I	GCAGC	6 cut(s)	242, 415, 580, 851, 1100, 1232
Bsu36I	CCTNAGG	1 cut(s)	1665
BsuRI	GGCC	3 cut(s)	617, 938, 1322
BtgI	CCRYGG	1 cut(s)	450
BtsCI	GGATG	9 cut(s)	253, 495, 658, 691, 732, 952, 1141, 1226, 1279
BtsIMutI	CAGTG	1 cut(s)	1389
BveI	ACCTGC	1 cut(s)	1683
Cac8I	GCNNGC	1 cut(s)	1527
CaiI	CAGNNNCTG	1 cut(s)	428
Cfr13I	GGNCC	2 cut(s)	273, 1658
Cfr42I	CCGCGG	1 cut(s)	453
CseI	GACGC	1 cut(s)	1226
Csp6I	GTAC	3 cut(s)	1199, 1292, 1670
CviQI	GTAC	3 cut(s)	1199, 1292, 1670
DdeI	CTNAG	6 cut(s)	281, 737, 804, 1154, 1665, 1690
DpnI	GATC	4 cut(s)	494, 539, 771, 1268
DpnII	GATC	4 cut(s)	492, 537, 769, 1266
DraI	TTTAAA	2 cut(s)	580, 1600
EaeI	YGGCCR	3 cut(s)	615, 936, 1320
Eam1104I	CTCTTC	3 cut(s)	583, 1034, 1537
EarI	CTCTTC	3 cut(s)	583, 1034, 1537
Ecl136II	GAGCTC	1 cut(s)	1151
Eco24I	GRGCYC	1 cut(s)	1153
Eco31I	GGTCTC	1 cut(s)	517
Eco32I	GATATC	1 cut(s)	658
Eco47I	GGWCC	2 cut(s)	273, 1658
Eco53kI	GAGCTC	1 cut(s)	1151
Eco57I	CTGAAG	1 cut(s)	363
Eco81I	CCTNAGG	1 cut(s)	1665
Eco91I	GGTNACC	1 cut(s)	868
EcoICRI	GAGCTC	1 cut(s)	1151
EcoO65I	GGTNACC	1 cut(s)	868
EcoRI	GAATTC	1 cut(s)	66
EcoRII	CCWGG	1 cut(s)	845
EcoRV	GATATC	1 cut(s)	658
EcoT22I	ATGCAT	1 cut(s)	734
EcoT38I	GRGCYC	1 cut(s)	1153
FaqI	GGGAC	1 cut(s)	945
FbaI	TGATCA	1 cut(s)	1266
FokI	GGATG	9 cut(s)	260, 482, 665, 698, 719, 959, 1148, 1213, 1286
FriOI	GRGCYC	1 cut(s)	1153
FspBI	CTAG	7 cut(s)	62, 125, 564, 675, 891, 1616, 1629
GsaI	CCCAGC	1 cut(s)	575
GsuI	CTGGAG	4 cut(s)	505, 734, 932, 1144
HaeIII	GGCC	3 cut(s)	617, 938, 1322
HapII	CCGG	3 cut(s)	683, 977, 1708
HgaI	GACGC	1 cut(s)	1226
HincII	GTYRAC	2 cut(s)	470, 1621
HindII	GTYRAC	2 cut(s)	470, 1621
HindIII	AAGCTT	2 cut(s)	151, 707
HinfI	GANTC	4 cut(s)	518, 692, 950, 1130
HpaII	CCGG	3 cut(s)	683, 977, 1708
HphI	GGTGA	5 cut(s)	92, 355, 880, 979, 1381
Hpy166II	GTNNAC	8 cut(s)	470, 599, 680, 1201, 1333, 1558, 1621, 1672
Hpy188I	TCNGA	6 cut(s)	382, 517, 774, 1135, 1308, 1591
Hpy188III	TCNNGA	5 cut(s)	713, 790, 823, 1161, 1172
Hpy8I	GTNNAC	8 cut(s)	470, 599, 680, 1201, 1333, 1558, 1621, 1672
Hpy99I	CGWCG	1 cut(s)	644
HpyAV	CCTTC	9 cut(s)	339, 415, 877, 1132, 1241, 1247, 1433, 1649, 1694
HpyCH4III	ACNGT	6 cut(s)	18, 665, 1048, 1312, 1387, 1635
HpyCH4IV	ACGT	2 cut(s)	905, 1649
HpyCH4V	TGCA	8 cut(s)	220, 242, 336, 460, 609, 732, 960, 1100
HpyF10VI	GCNNNNNNNGC	6 cut(s)	239, 980, 1088, 1097, 1347, 1502
HpyF3I	CTNAG	6 cut(s)	281, 737, 804, 1154, 1665, 1690
HpySE526I	ACGT	2 cut(s)	905, 1649
KpnI	GGTACC	1 cut(s)	1295
Ksp22I	TGATCA	1 cut(s)	1266
KspI	CCGCGG	1 cut(s)	453
Kzo9I	GATC	4 cut(s)	492, 537, 769, 1266
LguI	GCTCTTC	2 cut(s)	1034, 1537
LmnI	GCTCC	5 cut(s)	979, 1031, 1085, 1163, 1355
Lsp1109I	GCAGC	6 cut(s)	242, 415, 580, 851, 1100, 1232
LweI	GCATC	8 cut(s)	447, 676, 719, 741, 1108, 1177, 1232, 1483
MaeI	CTAG	7 cut(s)	62, 125, 564, 675, 891, 1616, 1629
MaeII	ACGT	2 cut(s)	905, 1649
MaeIII	GTNAC	8 cut(s)	3, 212, 868, 906, 1237, 1312, 1361, 1387
MalI	GATC	4 cut(s)	494, 539, 771, 1268
MboI	GATC	4 cut(s)	492, 537, 769, 1266
MfeI	CAATTG	1 cut(s)	1565
MhlI	GDGCHC	2 cut(s)	1046, 1153
MlsI	TGGCCA	3 cut(s)	617, 938, 1322
MluCI	AATT	8 cut(s)	66, 170, 349, 371, 611, 877, 1410, 1565
MluNI	TGGCCA	3 cut(s)	617, 938, 1322
MlyI	GAGTC	3 cut(s)	512, 686, 944
MmeI	TCCRAC	2 cut(s)	1459, 1632
Mox20I	TGGCCA	3 cut(s)	617, 938, 1322
Mph1103I	ATGCAT	1 cut(s)	734
MscI	TGGCCA	3 cut(s)	617, 938, 1322
MseI	TTAA	4 cut(s)	506, 579, 1248, 1599
MslI	CAYNNNNRTG	2 cut(s)	833, 965
Msp20I	TGGCCA	3 cut(s)	617, 938, 1322
MspA1I	CMGCKG	5 cut(s)	428, 452, 571, 944, 1190
MspCI	CTTAAG	1 cut(s)	505
MspI	CCGG	3 cut(s)	683, 977, 1708
MspR9I	CCNGG	1 cut(s)	847
MunI	CAATTG	1 cut(s)	1565
Mva1269I	GAATGC	1 cut(s)	1102
MvaI	CCWGG	1 cut(s)	847
MvnI	CGCG	2 cut(s)	452, 528
MwoI	GCNNNNNNNGC	6 cut(s)	239, 980, 1088, 1097, 1347, 1502
NdeII	GATC	4 cut(s)	492, 537, 769, 1266
NlaIV	GGNNCC	3 cut(s)	548, 975, 1293
NmeAIII	GCCGAG	1 cut(s)	834
NmuCI	GTSAC	3 cut(s)	868, 1237, 1387
NsiI	ATGCAT	1 cut(s)	734
NspI	RCATGY	2 cut(s)	960, 1180
OliI	CACNNNNGTG	1 cut(s)	965
PaqCI	CACCTGC	1 cut(s)	1683
PciSI	GCTCTTC	2 cut(s)	1034, 1537
PctI	GAATGC	1 cut(s)	1102
PfeI	GAWTC	1 cut(s)	1130
PleI	GAGTC	3 cut(s)	512, 686, 944
PpsI	GAGTC	3 cut(s)	512, 686, 944
PsiI	TTATAA	1 cut(s)	1511
Psp124BI	GAGCTC	1 cut(s)	1153
Psp1406I	AACGTT	1 cut(s)	905
Psp6I	CCWGG	1 cut(s)	845
PspEI	GGTNACC	1 cut(s)	868
PspFI	CCCAGC	1 cut(s)	571
PspGI	CCWGG	1 cut(s)	845
PspN4I	GGNNCC	3 cut(s)	548, 975, 1293
PspPI	GGNCC	2 cut(s)	273, 1658
PstNI	CAGNNNCTG	1 cut(s)	428
PvuII	CAGCTG	2 cut(s)	571, 1190
RsaI	GTAC	3 cut(s)	1200, 1293, 1671
RsaNI	GTAC	3 cut(s)	1199, 1292, 1670
RseI	CAYNNNNRTG	2 cut(s)	833, 965
SacI	GAGCTC	1 cut(s)	1153
SacII	CCGCGG	1 cut(s)	453
SapI	GCTCTTC	2 cut(s)	1034, 1537
SaqAI	TTAA	4 cut(s)	506, 579, 1248, 1599
Sau3AI	GATC	4 cut(s)	492, 537, 769, 1266
Sau96I	GGNCC	2 cut(s)	273, 1658
SchI	GAGTC	3 cut(s)	512, 686, 944
ScrFI	CCNGG	1 cut(s)	847
SduI	GDGCHC	2 cut(s)	1046, 1153
SfaNI	GCATC	8 cut(s)	447, 676, 719, 741, 1108, 1177, 1232, 1483
SfcI	CTRYAG	2 cut(s)	661, 1212
Sfr303I	CCGCGG	1 cut(s)	453
SgrBI	CCGCGG	1 cut(s)	453
SinI	GGWCC	2 cut(s)	273, 1658
SmiMI	CAYNNNNRTG	2 cut(s)	833, 965
SmlI	CTYRAG	4 cut(s)	37, 505, 790, 821
SmoI	CTYRAG	4 cut(s)	37, 505, 790, 821
SpeI	ACTAGT	1 cut(s)	1615
Sse9I	AATT	8 cut(s)	66, 170, 349, 371, 611, 877, 1410, 1565
SspMI	CTAG	7 cut(s)	62, 125, 564, 675, 891, 1616, 1629
SstI	GAGCTC	1 cut(s)	1153
StyD4I	CCNGG	1 cut(s)	845
TaaI	ACNGT	6 cut(s)	18, 665, 1048, 1312, 1387, 1635
TaiI	ACGT	2 cut(s)	908, 1652
TaqI	TCGA	2 cut(s)	540, 1552
TasI	AATT	8 cut(s)	66, 170, 349, 371, 611, 877, 1410, 1565
TauI	GCSGC	6 cut(s)	304, 428, 452, 455, 528, 1341
TfiI	GAWTC	1 cut(s)	1130
Tru1I	TTAA	4 cut(s)	506, 579, 1248, 1599
Tru9I	TTAA	4 cut(s)	506, 579, 1248, 1599
TscAI	CASTG	1 cut(s)	1396
TseFI	GTSAC	3 cut(s)	868, 1237, 1387
TseI	GCWGC	6 cut(s)	230, 428, 568, 839, 1088, 1220
Tsp45I	GTSAC	3 cut(s)	868, 1237, 1387
TspDTI	ATGAA	6 cut(s)	162, 321, 521, 743, 935, 1434
TspRI	CASTG	1 cut(s)	1396
Vha464I	CTTAAG	1 cut(s)	505
VpaK11BI	GGWCC	2 cut(s)	273, 1658
XapI	RAATTY	3 cut(s)	66, 170, 877
XceI	RCATGY	2 cut(s)	960, 1180
XspI	CTAG	7 cut(s)	62, 125, 564, 675, 891, 1616, 1629
Zsp2I	ATGCAT	1 cut(s)	734

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.