pycom05g19040

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007046
Physical Location & Seq
Forward (+)
21952200 .. 21956327
4128 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19040.6

Sequence Viewer

Length: 1134 bp
ATGATTACTCAGATCTTGAGCTGTGATAATGAGCCTAGAAAGAAGCTCAAGAACATTGTCATGGCAGCAACCAGAGAACAGAGATTCGAGAGAGTGACGAAAAATTTGAAGGTGGCTAGAGTTTTCACTACCTTAGTGGAAGAGATGAAGGCAATGGGACTCGCAGCGCCAGATGACTCGACGTGTACAGAGGTGATGGCTCCGGTGGCTCACGCTGATAGAAGTCCGGTCCTACTCCTCATGGGAGGCGGAATGGGAGCAGGGAAGAGCACTGTGTTAAAGGACATTCTCAAAGAACCGTTCTGGGCTGGAGCAGCGGGGAATGCAGTAGTGATTGAGGCAGATGCCTTCAAAGAATCAGATGTCATATACAGAGCTCTCAGCTCCAGAGGCCATCATGATGACATGCTTCAAACCGCTGAACTGGTACACCAATCATCTACGGACGCAGCATCATCCCTCCTCGTCACAGCGCTTAATGAAGGGCGGGATGTCATCATGGACGGCACACTCTCTTGGGTGCCCTTTGTTGTGCAGACAATAACAATGGCCAGAAATGTTCATCGCCGCCGTTACCGCATGGGACCTGGTTACAGGGTGAATGAGGATGGAACTGTCACTGAAAACTATTGGGAACGACTTGATGATGAAGAACCAGAACAAGTAGGAGGCAGAAAGAGAAAACCATACAGGATAGAGCTCGTTGGAGTCGTGTGTGATGCTTATCTAGCTGTTATAAGAGGCATAAGGAGAGCTATCATGGTTAGAAGAGCGGTGCGAGTAAACTCACAATTGAAATCCCACAAGAGATTTGCAAATTCATATCTAACGTATTGCCAACTGGTTGACAACGCGAGACTATATTGCACGAATGCATTGGAAGGCCCGCCTAAGTTGATAGGATGGAAGGACAAAGACAGAACATTGCTTGTGGATCCAGACGAAATCGACTGCTTAAAAAGGGTGGGCAGGTTAAATGACATGGCAAATTCCATATACGAACTTTACAAGCACCCTAACCCAGCTTGTAAAACCGGGTCAGTCTGGAAGGACATTGTCTTATCGCCTTCAAGATTAAACATTCAGAAGGAGCTGAAGTACTCCATCCAGAAAGTTGAAAGATTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

378

Amino Acids

42.49

Weight (kDa)

9.17

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 960
AccB1I GGYRCC 1 cut(s) 520
AccBSI CCGCTC 1 cut(s) 773
AccII CGCG 1 cut(s) 854
AciI CCGC 8 cut(s) 249, 317, 417, 487, 568, 577, 773, 887
AclWI GGATC 2 cut(s) 929, 942
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 3 cut(s) 103, 817, 988
AcuI CTGAAG 1 cut(s) 1115
AfaI GTAC 3 cut(s) 187, 429, 1100
AfeI AGCGCT 1 cut(s) 474
AflIII ACRYGT 1 cut(s) 182
AgsI TTSAA 7 cut(s) 109, 352, 413, 796, 1071, 1118, 1126
AjiI CACGTC 1 cut(s) 183
AjnI CCWGG 1 cut(s) 586
AluBI AGCT 9 cut(s) 21, 46, 377, 384, 700, 731, 755, 1025, 1093
AluI AGCT 9 cut(s) 21, 46, 377, 384, 700, 731, 755, 1025, 1093
Alw21I GWGCWC 3 cut(s) 272, 379, 702
Alw26I GTCTC 1 cut(s) 850
AlwI GGATC 2 cut(s) 929, 942
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 3 cut(s) 391, 549, 883
ApeKI GCWGC 4 cut(s) 65, 164, 314, 449
ApoI RAATTY 3 cut(s) 103, 817, 988
ArsI GACNNNNNNTTYG 2 cut(s) 88, 120
AspLEI GCGC 2 cut(s) 169, 475
AspS9I GGNCC 3 cut(s) 229, 584, 884
AsuC2I CCSGG 1 cut(s) 1036
AsuHPI GGTGA 2 cut(s) 205, 610
AvaII GGWCC 2 cut(s) 229, 584
BaeGI GKGCMC 1 cut(s) 525
BalI TGGCCA 1 cut(s) 551
BamHI GGATCC 1 cut(s) 934
BanI GGYRCC 1 cut(s) 520
BanII GRGCYC 2 cut(s) 379, 702
Bbv12I GWGCWC 3 cut(s) 272, 379, 702
BbvI GCAGC 4 cut(s) 77, 176, 326, 461
BccI CCATC 5 cut(s) 190, 402, 602, 897, 1112
BceAI ACGGC 2 cut(s) 520, 555
BciT130I CCWGG 1 cut(s) 588
BcnI CCSGG 1 cut(s) 1036
BcoDI GTCTC 1 cut(s) 850
BfaI CTAG 3 cut(s) 36, 117, 728
BfoI RGCGCY 2 cut(s) 170, 476
BfuAI ACCTGC 1 cut(s) 960
BglII AGATCT 1 cut(s) 12
BisI GCNGC 5 cut(s) 66, 165, 315, 450, 568
BlsI GCNGC 5 cut(s) 67, 166, 316, 451, 569
BmcAI AGTACT 1 cut(s) 1100
Bme1390I CCNGG 2 cut(s) 588, 1036
Bme18I GGWCC 2 cut(s) 229, 584
BmgBI CACGTC 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 229, 584, 884
BmiI GGNNCC 4 cut(s) 201, 522, 585, 936
BmrFI CCNGG 2 cut(s) 588, 1036
BmsI GCATC 3 cut(s) 334, 461, 709
BpmI CTGGAG 2 cut(s) 330, 370
BpuEI CTTGAG 2 cut(s) 32, 37
BpuMI CCSGG 1 cut(s) 1036
BsaBI GATNNNNATC 1 cut(s) 723
BsaWI WCCGGW 2 cut(s) 202, 226
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bse1I ACTGG 2 cut(s) 429, 846
Bse3DI GCAATG 2 cut(s) 159, 923
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 588
BseGI GGATG 5 cut(s) 455, 496, 613, 908, 1104
BseJI GATNNNNATC 1 cut(s) 723
BseMI GCAATG 2 cut(s) 159, 923
BseMII CTCAG 2 cut(s) 23, 394
BseNI ACTGG 2 cut(s) 429, 846
BseRI GAGGAG 2 cut(s) 227, 452
BseSI GKGCMC 1 cut(s) 525
BseXI GCAGC 4 cut(s) 77, 176, 326, 461
BseYI CCCAGC 1 cut(s) 1021
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 3 cut(s) 393, 551, 885
BshNI GGYRCC 1 cut(s) 520
BsiHKAI GWGCWC 3 cut(s) 272, 379, 702
BsiSI CCGG 3 cut(s) 203, 227, 1035
BslFI GGGAC 2 cut(s) 171, 597
BsmAI GTCTC 1 cut(s) 850
BsmFI GGGAC 2 cut(s) 171, 597
BsmI GAATGC 2 cut(s) 328, 877
BsnI GGCC 3 cut(s) 393, 551, 885
Bsp1286I GDGCHC 4 cut(s) 272, 379, 525, 702
Bsp1407I TGTACA 1 cut(s) 185
Bsp143I GATC 2 cut(s) 12, 934
BspACI CCGC 8 cut(s) 249, 317, 417, 487, 568, 577, 773, 887
BspANI GGCC 3 cut(s) 393, 551, 885
BspCNI CTCAG 2 cut(s) 22, 393
BspFNI CGCG 1 cut(s) 854
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 4 cut(s) 201, 522, 585, 936
BspMI ACCTGC 1 cut(s) 960
BspPI GGATC 2 cut(s) 929, 942
BspQI GCTCTTC 2 cut(s) 260, 763
BspT107I GGYRCC 1 cut(s) 520
BsrBI CCGCTC 1 cut(s) 773
BsrDI GCAATG 2 cut(s) 159, 923
BsrGI TGTACA 1 cut(s) 185
BsrI ACTGG 2 cut(s) 429, 846
BssMI GATC 2 cut(s) 12, 934
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 3 cut(s) 274, 300, 616
Bst6I CTCTTC 3 cut(s) 135, 260, 763
BstAUI TGTACA 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 887
BstDEI CTNAG 4 cut(s) 9, 133, 380, 891
BstF5I GGATG 5 cut(s) 455, 496, 613, 908, 1104
BstFNI CGCG 1 cut(s) 854
BstH2I RGCGCY 2 cut(s) 170, 476
BstHHI GCGC 2 cut(s) 169, 475
BstKTI GATC 2 cut(s) 15, 937
BstMAI GTCTC 1 cut(s) 850
BstMBI GATC 2 cut(s) 12, 934
BstMWI GCNNNNNNNGC 6 cut(s) 206, 314, 323, 390, 576, 728
BstNI CCWGG 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 2 cut(s) 586, 1034
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 4 cut(s) 77, 176, 326, 461
BstX2I RGATCY 2 cut(s) 12, 934
BstYI RGATCY 2 cut(s) 12, 934
BsuRI GGCC 3 cut(s) 393, 551, 885
BtgZI GCGATG 1 cut(s) 548
BtrI CACGTC 1 cut(s) 183
BtsCI GGATG 5 cut(s) 455, 496, 613, 908, 1104
BtsIMutI CAGTG 2 cut(s) 270, 618
BveI ACCTGC 1 cut(s) 960
Cac8I GCNNGC 1 cut(s) 887
CciI TCATGA 1 cut(s) 397
CfoI GCGC 2 cut(s) 169, 475
Cfr13I GGNCC 3 cut(s) 229, 584, 884
CseI GACGC 1 cut(s) 455
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 3 cut(s) 186, 428, 1099
CviAII CATG 8 cut(s) 61, 241, 398, 406, 499, 580, 760, 982
CviQI GTAC 3 cut(s) 186, 428, 1099
DdeI CTNAG 4 cut(s) 9, 133, 380, 891
DpnI GATC 2 cut(s) 14, 936
DpnII GATC 2 cut(s) 12, 934
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 3 cut(s) 135, 260, 763
EarI CTCTTC 3 cut(s) 135, 260, 763
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 2 cut(s) 377, 700
Eco24I GRGCYC 2 cut(s) 379, 702
Eco47I GGWCC 2 cut(s) 229, 584
Eco47III AGCGCT 1 cut(s) 474
Eco53kI GAGCTC 2 cut(s) 377, 700
Eco57I CTGAAG 1 cut(s) 1115
EcoICRI GAGCTC 2 cut(s) 377, 700
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 586
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 2 cut(s) 379, 702
FaeI CATG 8 cut(s) 64, 244, 401, 409, 502, 583, 763, 985
FaqI GGGAC 2 cut(s) 171, 597
FatI CATG 8 cut(s) 60, 240, 397, 405, 498, 579, 759, 981
FauI CCCGC 3 cut(s) 310, 480, 894
Fnu4HI GCNGC 5 cut(s) 66, 165, 315, 450, 568
FokI GGATG 5 cut(s) 442, 503, 620, 915, 1091
FriOI GRGCYC 2 cut(s) 379, 702
Fsp4HI GCNGC 5 cut(s) 66, 165, 315, 450, 568
FspBI CTAG 3 cut(s) 36, 117, 728
GlaI GCGC 2 cut(s) 168, 474
GluI GCNGC 5 cut(s) 66, 165, 315, 450, 568
GsaI CCCAGC 1 cut(s) 1025
GsuI CTGGAG 2 cut(s) 330, 370
HaeII RGCGCY 2 cut(s) 170, 476
HaeIII GGCC 3 cut(s) 393, 551, 885
HapII CCGG 3 cut(s) 203, 227, 1035
HgaI GACGC 1 cut(s) 455
HhaI GCGC 2 cut(s) 169, 475
Hin1II CATG 8 cut(s) 64, 244, 401, 409, 502, 583, 763, 985
Hin6I GCGC 2 cut(s) 167, 473
HinP1I GCGC 2 cut(s) 167, 473
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HinfI GANTC 5 cut(s) 84, 159, 176, 356, 708
HpaII CCGG 3 cut(s) 203, 227, 1035
HphI GGTGA 2 cut(s) 205, 610
Hpy166II GTNNAC 4 cut(s) 186, 430, 784, 847
Hpy188I TCNGA 3 cut(s) 12, 361, 1086
Hpy188III TCNNGA 9 cut(s) 16, 49, 88, 387, 398, 938, 1045, 1071, 1108
Hpy8I GTNNAC 4 cut(s) 186, 430, 784, 847
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 9 cut(s) 103, 142, 358, 476, 875, 901, 1042, 1077, 1081
HpyCH4III ACNGT 3 cut(s) 274, 300, 616
HpyCH4IV ACGT 2 cut(s) 182, 830
HpyCH4V TGCA 5 cut(s) 326, 535, 815, 867, 875
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 314, 323, 390, 576, 728
HpyF3I CTNAG 4 cut(s) 9, 133, 380, 891
HpySE526I ACGT 2 cut(s) 182, 830
Hsp92II CATG 8 cut(s) 64, 244, 401, 409, 502, 583, 763, 985
HspAI GCGC 2 cut(s) 167, 473
Kzo9I GATC 2 cut(s) 12, 934
LguI GCTCTTC 2 cut(s) 260, 763
LmnI GCTCC 5 cut(s) 205, 257, 311, 389, 1090
Lsp1109I GCAGC 4 cut(s) 77, 176, 326, 461
LweI GCATC 3 cut(s) 334, 461, 709
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 3 cut(s) 36, 117, 728
MaeII ACGT 2 cut(s) 182, 830
MaeIII GTNAC 5 cut(s) 94, 466, 572, 590, 616
MalI GATC 2 cut(s) 14, 936
MbiI CCGCTC 1 cut(s) 773
MboI GATC 2 cut(s) 12, 934
MboII GAAGA 4 cut(s) 152, 277, 662, 780
MfeI CAATTG 1 cut(s) 791
MflI RGATCY 2 cut(s) 12, 934
MhlI GDGCHC 4 cut(s) 272, 379, 525, 702
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 4 cut(s) 103, 791, 817, 988
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 3 cut(s) 153, 170, 717
MmeI TCCRAC 1 cut(s) 685
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 1 cut(s) 877
MscI TGGCCA 1 cut(s) 551
MseI TTAA 5 cut(s) 278, 477, 956, 974, 1076
MslI CAYNNNNRTG 2 cut(s) 59, 399
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 2 cut(s) 317, 419
MspI CCGG 3 cut(s) 203, 227, 1035
MspR9I CCNGG 2 cut(s) 588, 1036
MunI CAATTG 1 cut(s) 791
Mva1269I GAATGC 2 cut(s) 328, 877
MvaI CCWGG 1 cut(s) 588
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 6 cut(s) 206, 314, 323, 390, 576, 728
NciI CCSGG 1 cut(s) 1036
NdeII GATC 2 cut(s) 12, 934
NlaIII CATG 8 cut(s) 64, 244, 401, 409, 502, 583, 763, 985
NlaIV GGNNCC 4 cut(s) 201, 522, 585, 936
NmuCI GTSAC 3 cut(s) 94, 466, 616
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 1 cut(s) 409
PagI TCATGA 1 cut(s) 397
PciSI GCTCTTC 2 cut(s) 260, 763
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PctI GAATGC 2 cut(s) 328, 877
PfeI GAWTC 2 cut(s) 84, 356
PflFI GACNNNGTC 1 cut(s) 1055
PkrI GCNGC 5 cut(s) 67, 166, 316, 451, 569
PleI GAGTC 3 cut(s) 153, 170, 716
PpsI GAGTC 3 cut(s) 153, 170, 716
PpuMI RGGWCCY 1 cut(s) 584
PsiI TTATAA 1 cut(s) 737
Psp124BI GAGCTC 2 cut(s) 379, 702
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 586
PspFI CCCAGC 1 cut(s) 1021
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 4 cut(s) 201, 522, 585, 936
PspPI GGNCC 3 cut(s) 229, 584, 884
PspPPI RGGWCCY 1 cut(s) 584
PsuI RGATCY 2 cut(s) 12, 934
PsyI GACNNNGTC 1 cut(s) 1055
RsaI GTAC 3 cut(s) 187, 429, 1100
RsaNI GTAC 3 cut(s) 186, 428, 1099
RseI CAYNNNNRTG 2 cut(s) 59, 399
SacI GAGCTC 2 cut(s) 379, 702
SapI GCTCTTC 2 cut(s) 260, 763
SaqAI TTAA 5 cut(s) 278, 477, 956, 974, 1076
SatI GCNGC 5 cut(s) 66, 165, 315, 450, 568
Sau3AI GATC 2 cut(s) 12, 934
Sau96I GGNCC 3 cut(s) 229, 584, 884
ScaI AGTACT 1 cut(s) 1100
SchI GAGTC 3 cut(s) 153, 170, 717
ScrFI CCNGG 2 cut(s) 588, 1036
SduI GDGCHC 4 cut(s) 272, 379, 525, 702
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 3 cut(s) 334, 461, 709
SinI GGWCC 2 cut(s) 229, 584
SmiMI CAYNNNNRTG 2 cut(s) 59, 399
SmlI CTYRAG 2 cut(s) 16, 47
SmoI CTYRAG 2 cut(s) 16, 47
Sse9I AATT 4 cut(s) 103, 791, 817, 988
SsiI CCGC 8 cut(s) 249, 317, 417, 487, 568, 577, 773, 887
SspMI CTAG 3 cut(s) 36, 117, 728
SstI GAGCTC 2 cut(s) 379, 702
StyD4I CCNGG 2 cut(s) 586, 1034
TaaI ACNGT 3 cut(s) 274, 300, 616
TaiI ACGT 2 cut(s) 185, 833
TaqI TCGA 3 cut(s) 87, 179, 948
TasI AATT 4 cut(s) 103, 791, 817, 988
TatI WGTACW 2 cut(s) 185, 1098
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 2 cut(s) 84, 356
Tru1I TTAA 5 cut(s) 278, 477, 956, 974, 1076
Tru9I TTAA 5 cut(s) 278, 477, 956, 974, 1076
TscAI CASTG 2 cut(s) 277, 625
TseFI GTSAC 3 cut(s) 94, 466, 616
TseI GCWGC 4 cut(s) 65, 164, 314, 449
Tsp45I GTSAC 3 cut(s) 94, 466, 616
TspDTI ATGAA 5 cut(s) 161, 495, 551, 663, 810
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 2 cut(s) 277, 625
Tth111I GACNNNGTC 1 cut(s) 1055
VpaK11BI GGWCC 2 cut(s) 229, 584
XapI RAATTY 3 cut(s) 103, 817, 988
XceI RCATGY 1 cut(s) 409
XspI CTAG 3 cut(s) 36, 117, 728
ZrmI AGTACT 1 cut(s) 1100
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.