FvH4_2g22270

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007046
Physical Location & Seq
Forward (+)
18293442 .. 18296749
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22270.t1

Sequence Viewer

Length: 1536 bp
ATGCAGACAGATGAAAACAACAGCAAAACCACCTTGGCACAAATCTTGTTAGCCGTTGCCCTCGGCTTCATCATTGCCGCGGTGATGCATTATCGTCAACGAAAGATTAAACATGAGAAGATCATTCCACGCTTAAGGGTCTCGGACTCCAGCCGTGTTCATAAGATCGAAAGGTTCCCCCATTATGTAGCTAGGCAGCTGGGATTTCAAGATAGAAGAGAATGTCCAAACCTCTGTAAATTGGCCACCGAATACATGAGAAAATGCGACGGCTTTGAGGATGATATCTACAGTTTCTTTTCTAGTGAACCGGATGCAGATTCGTTGTTTGTGAAGCTTCTGGAGGAATTTGAGAGATGCATCCTCAGTTATTTTGCATTTCATTGGAACGAAGCTGATCTAATGATTAGTCAGGTCTTGAGTTGTGATACTGATCAGCCGAGGAAGAAGCTTAAGAACATTGTCATGGCAGCGACCAGGGAACAAAGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTAGAGGAGATGAAAGCAATGGGACTAGCCGCGGTGGATGACTCGACATGTACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTCATGGGAGGTGGAATGGGAGCAGGAAAAAGCACTGTGCTTAAGGACATTCTCAAAGAGCCATTCTGGGCCGGAGCAGCAGGGAATGCAGTAGTGATCGAAGCAGATGCCTTTAAGGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAATCATCTACAGACGCAGCATCATCCCTTCTGGTCACAGCACTTAACGAAGGGCGAGATGTGATCATGGATGGCACACTCTCATGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGGCGTTATCGCATGGGAGCTGGTTATAGGGTGAATGATGATGGAACTGTCATTGAAAACTATTGGGAACGAATCGAAGATGAAGAACCAGAACATGTAGAAGGCAGAAAGAGAAAACCATACAGGATAGAGCTCGTTGGAGTTGTGTGTGATGCTTATCTGGCTGTTATAAGAGGAATAAGGAGAGCTATCGTGTGTAGAAGAGCTGTTCGAGTAAACTCACAACTGAAATCCCACAAGAGATTTTCCGAGGCATTTAAAACATATTGCCAAATAGTTGACAATGCTAGACTATATAGCACTAATACCTTGGAAGGGCCACCTAAGTTGATAGGATGGAAAGACAAAGACAAGACCTTGCTGGTTGATCCAGATGAAATCGATTGCTTAAAAAGGGTGGCCAGATTAAATGACAGAGCGACTTCGATATATGAACTTTACAAGCACCCTAACCCAGCTTGTCAAAAGGGGTCAGTTTGGAAGGACATTGTCTTGTCACCTTCGAGGGTAAACATTCAACAGGAGCTGAAGTACTCTATCCAGAAAGTAGAAAGATTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

512

Amino Acids

58.37

Weight (kDa)

8.86

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 206 - 309 2.2e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
Acc65I GGTACC 1 cut(s) 922
AccB1I GGYRCC 1 cut(s) 922
AccII CGCG 2 cut(s) 80, 572
AciI CCGC 5 cut(s) 78, 80, 570, 572, 967
AclI AACGTT 1 cut(s) 536
AclWI GGATC 1 cut(s) 1331
AcoI YGGCCR 3 cut(s) 243, 951, 1368
AcsI RAATTY 2 cut(s) 347, 508
AcuI CTGAAG 1 cut(s) 1517
AfaI GTAC 4 cut(s) 592, 831, 924, 1502
AfiI CCNNNNNNNGG 2 cut(s) 646, 1284
AflII CTTAAG 3 cut(s) 133, 452, 683
AflIII ACRYGT 2 cut(s) 587, 1063
AgsI TTSAA 7 cut(s) 209, 494, 514, 815, 1025, 1487, 1528
AjnI CCWGG 1 cut(s) 476
Alw21I GWGCWC 2 cut(s) 784, 1104
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 1 cut(s) 1331
AlwNI CAGNNNCTG 1 cut(s) 1495
AoxI GGCC 5 cut(s) 243, 711, 951, 1286, 1368
ApeKI GCWGC 4 cut(s) 196, 470, 719, 851
ApoI RAATTY 2 cut(s) 347, 508
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
Asp718I GGTACC 1 cut(s) 922
AspS9I GGNCC 2 cut(s) 711, 1286
AsuHPI GGTGA 5 cut(s) 94, 511, 610, 1012, 1458
BalI TGGCCA 3 cut(s) 245, 953, 1370
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 2 cut(s) 784, 1104
Bbv12I GWGCWC 2 cut(s) 784, 1104
BbvI GCAGC 4 cut(s) 208, 482, 731, 863
BccI CCATC 4 cut(s) 595, 899, 1004, 1299
BceAI ACGGC 3 cut(s) 38, 138, 286
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 2 cut(s) 433, 897
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 6 cut(s) 192, 303, 522, 542, 566, 1257
BfmI CTRYAG 2 cut(s) 289, 843
BfrI CTTAAG 3 cut(s) 133, 452, 683
BisI GCNGC 6 cut(s) 78, 197, 471, 570, 720, 852
BlsI GCNGC 6 cut(s) 79, 198, 472, 571, 721, 853
BmcAI AGTACT 1 cut(s) 1502
Bme1390I CCNGG 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 711, 1286
BmiI GGNNCC 3 cut(s) 176, 606, 924
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 8 cut(s) 75, 304, 347, 369, 739, 808, 863, 1111
BpmI CTGGAG 3 cut(s) 133, 362, 775
BpuEI CTTGAG 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 1350
BsaBI GATNNNNATC 2 cut(s) 432, 1125
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 8 cut(s) 33, 61, 78, 440, 477, 570, 1218, 1278
BsaWI WCCGGW 2 cut(s) 310, 607
BsaXI ACNNNNNCTCC 2 cut(s) 1484, 1514
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 1284
Bse118I RCCGGY 1 cut(s) 969
Bse1I ACTGG 2 cut(s) 632, 831
Bse3DI GCAATG 2 cut(s) 72, 564
Bse8I GATNNNNATC 2 cut(s) 432, 1125
BseBI CCWGG 1 cut(s) 478
BseCI ATCGAT 1 cut(s) 1350
BseDI CCNNGG 8 cut(s) 33, 61, 78, 440, 477, 570, 1218, 1278
BseGI GGATG 8 cut(s) 286, 319, 360, 583, 772, 857, 910, 1310
BseJI GATNNNNATC 2 cut(s) 432, 1125
BseLI CCNNNNNNNGG 2 cut(s) 646, 1284
BseMI GCAATG 2 cut(s) 72, 564
BseMII CTCAG 2 cut(s) 379, 799
BseNI ACTGG 2 cut(s) 632, 831
BseRI GAGGAG 2 cut(s) 560, 629
BseXI GCAGC 4 cut(s) 208, 482, 731, 863
BseYI CCCAGC 2 cut(s) 199, 1423
Bsh1236I CGCG 2 cut(s) 80, 572
BshFI GGCC 5 cut(s) 245, 713, 953, 1288, 1370
BshNI GGYRCC 1 cut(s) 922
BshVI ATCGAT 1 cut(s) 1350
BsiHKAI GWGCWC 2 cut(s) 784, 1104
BsiSI CCGG 4 cut(s) 311, 608, 714, 970
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 2 cut(s) 646, 1284
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 5 cut(s) 245, 713, 953, 1288, 1370
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 784, 1104
Bsp1407I TGTACA 1 cut(s) 590
Bsp143I GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
BspACI CCGC 5 cut(s) 78, 80, 570, 572, 967
BspANI GGCC 5 cut(s) 245, 713, 953, 1288, 1370
BspCNI CTCAG 2 cut(s) 378, 798
BspDI ATCGAT 1 cut(s) 1350
BspFNI CGCG 2 cut(s) 80, 572
BspLI GGNNCC 3 cut(s) 176, 606, 924
BspPI GGATC 1 cut(s) 1331
BspQI GCTCTTC 1 cut(s) 1165
BspT107I GGYRCC 1 cut(s) 922
BspTI CTTAAG 3 cut(s) 133, 452, 683
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 72, 564
BsrFI RCCGGY 1 cut(s) 969
BsrGI TGTACA 1 cut(s) 590
BsrI ACTGG 2 cut(s) 632, 831
BssAI RCCGGY 1 cut(s) 969
BssECI CCNNGG 8 cut(s) 33, 61, 78, 440, 477, 570, 1218, 1278
BssMI GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
BssT1I CCWWGG 2 cut(s) 33, 1278
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 4 cut(s) 293, 679, 943, 1018
Bst6I CTCTTC 3 cut(s) 211, 647, 1165
BstAFI CTTAAG 3 cut(s) 133, 452, 683
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 971
BstDEI CTNAG 3 cut(s) 365, 785, 1293
BstDSI CCRYGG 2 cut(s) 78, 570
BstEII GGTNACC 1 cut(s) 499
BstF5I GGATG 8 cut(s) 286, 319, 360, 583, 772, 857, 910, 1310
BstFNI CGCG 2 cut(s) 80, 572
BstKTI GATC 7 cut(s) 123, 168, 400, 436, 741, 900, 1339
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
BstMWI GCNNNNNNNGC 4 cut(s) 611, 719, 728, 1130
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 3 cut(s) 591, 811, 1067
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 2 cut(s) 289, 843
BstUI CGCG 2 cut(s) 80, 572
BstV1I GCAGC 4 cut(s) 208, 482, 731, 863
Bsu15I ATCGAT 1 cut(s) 1350
BsuRI GGCC 5 cut(s) 245, 713, 953, 1288, 1370
BsuTUI ATCGAT 1 cut(s) 1350
BtgI CCRYGG 2 cut(s) 78, 570
BtsCI GGATG 8 cut(s) 286, 319, 360, 583, 772, 857, 910, 1310
BtsIMutI CAGTG 1 cut(s) 675
Cac8I GCNNGC 1 cut(s) 971
CaiI CAGNNNCTG 1 cut(s) 1495
Cfr10I RCCGGY 1 cut(s) 969
Cfr13I GGNCC 2 cut(s) 711, 1286
Cfr42I CCGCGG 2 cut(s) 81, 573
ClaI ATCGAT 1 cut(s) 1350
CseI GACGC 1 cut(s) 857
Csp6I GTAC 4 cut(s) 591, 830, 923, 1501
CspCI CAANNNNNGTGG 2 cut(s) 1278, 1313
CviQI GTAC 4 cut(s) 591, 830, 923, 1501
DdeI CTNAG 3 cut(s) 365, 785, 1293
DpnI GATC 7 cut(s) 122, 167, 399, 435, 740, 899, 1338
DpnII GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
DraI TTTAAA 1 cut(s) 1228
EaeI YGGCCR 3 cut(s) 243, 951, 1368
Eam1104I CTCTTC 3 cut(s) 211, 647, 1165
EarI CTCTTC 3 cut(s) 211, 647, 1165
Ecl136II GAGCTC 2 cut(s) 782, 1102
Eco130I CCWWGG 2 cut(s) 33, 1278
Eco24I GRGCYC 2 cut(s) 784, 1104
Eco31I GGTCTC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 286
Eco53kI GAGCTC 2 cut(s) 782, 1102
Eco57I CTGAAG 1 cut(s) 1517
Eco91I GGTNACC 1 cut(s) 499
EcoICRI GAGCTC 2 cut(s) 782, 1102
EcoO65I GGTNACC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 2 cut(s) 33, 1278
EcoT22I ATGCAT 2 cut(s) 90, 362
EcoT38I GRGCYC 2 cut(s) 784, 1104
ErhI CCWWGG 2 cut(s) 33, 1278
FaqI GGGAC 1 cut(s) 576
FbaI TGATCA 2 cut(s) 433, 897
Fnu4HI GCNGC 6 cut(s) 78, 197, 471, 570, 720, 852
FokI GGATG 8 cut(s) 293, 326, 347, 590, 779, 844, 917, 1317
FriOI GRGCYC 2 cut(s) 784, 1104
Fsp4HI GCNGC 6 cut(s) 78, 197, 471, 570, 720, 852
FspBI CTAG 6 cut(s) 192, 303, 522, 542, 566, 1257
GluI GCNGC 6 cut(s) 78, 197, 471, 570, 720, 852
GsaI CCCAGC 2 cut(s) 203, 1427
GsuI CTGGAG 3 cut(s) 133, 362, 775
HaeIII GGCC 5 cut(s) 245, 713, 953, 1288, 1370
HapII CCGG 4 cut(s) 311, 608, 714, 970
HgaI GACGC 1 cut(s) 857
HincII GTYRAC 2 cut(s) 98, 1249
HindII GTYRAC 2 cut(s) 98, 1249
HindIII AAGCTT 2 cut(s) 335, 449
HinfI GANTC 5 cut(s) 146, 320, 581, 761, 1041
HpaII CCGG 4 cut(s) 311, 608, 714, 970
HphI GGTGA 5 cut(s) 94, 511, 610, 1012, 1458
Hpy166II GTNNAC 7 cut(s) 98, 308, 832, 964, 1186, 1249, 1480
Hpy188I TCNGA 4 cut(s) 145, 766, 939, 1219
Hpy188III TCNNGA 7 cut(s) 209, 341, 418, 792, 803, 1340, 1510
Hpy8I GTNNAC 7 cut(s) 98, 308, 832, 964, 1186, 1249, 1480
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 7 cut(s) 508, 872, 878, 1064, 1277, 1444, 1479
HpyCH4III ACNGT 4 cut(s) 293, 679, 943, 1018
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 6 cut(s) 4, 88, 317, 360, 377, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 611, 719, 728, 1130
HpyF3I CTNAG 3 cut(s) 365, 785, 1293
HpySE526I ACGT 1 cut(s) 536
KpnI GGTACC 1 cut(s) 926
KroI GCCGGC 1 cut(s) 969
KroNI GCCGGC 1 cut(s) 971
Ksp22I TGATCA 2 cut(s) 433, 897
KspI CCGCGG 2 cut(s) 81, 573
Kzo9I GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
LguI GCTCTTC 1 cut(s) 1165
LmnI GCTCC 6 cut(s) 610, 662, 716, 794, 986, 1492
Lsp1109I GCAGC 4 cut(s) 208, 482, 731, 863
LweI GCATC 8 cut(s) 75, 304, 347, 369, 739, 808, 863, 1111
MaeI CTAG 6 cut(s) 192, 303, 522, 542, 566, 1257
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 5 cut(s) 499, 537, 868, 943, 1464
MalI GATC 7 cut(s) 122, 167, 399, 435, 740, 899, 1338
MboI GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
MboII GAAGA 7 cut(s) 130, 228, 457, 634, 1058, 1064, 1182
MhlI GDGCHC 2 cut(s) 784, 1104
MlsI TGGCCA 3 cut(s) 245, 953, 1370
MluCI AATT 3 cut(s) 239, 347, 508
MluNI TGGCCA 3 cut(s) 245, 953, 1370
MlyI GAGTC 2 cut(s) 140, 575
MmeI TCCRAC 1 cut(s) 1087
Mox20I TGGCCA 3 cut(s) 245, 953, 1370
Mph1103I ATGCAT 2 cut(s) 90, 362
MreI CGCCGGCG 1 cut(s) 969
MroNI GCCGGC 1 cut(s) 969
MscI TGGCCA 3 cut(s) 245, 953, 1370
MseI TTAA 9 cut(s) 108, 134, 453, 684, 756, 879, 1227, 1358, 1376
MslI CAYNNNNRTG 2 cut(s) 464, 916
Msp20I TGGCCA 3 cut(s) 245, 953, 1370
MspA1I CMGCKG 4 cut(s) 80, 199, 572, 821
MspCI CTTAAG 3 cut(s) 133, 452, 683
MspI CCGG 4 cut(s) 311, 608, 714, 970
MspR9I CCNGG 1 cut(s) 478
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 2 cut(s) 80, 572
MwoI GCNNNNNNNGC 4 cut(s) 611, 719, 728, 1130
NaeI GCCGGC 1 cut(s) 971
NdeII GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
NgoMIV GCCGGC 1 cut(s) 969
NlaIV GGNNCC 3 cut(s) 176, 606, 924
NmeAIII GCCGAG 2 cut(s) 42, 465
NmuCI GTSAC 3 cut(s) 499, 868, 1464
NsiI ATGCAT 2 cut(s) 90, 362
NspI RCATGY 3 cut(s) 591, 811, 1067
PciI ACATGT 2 cut(s) 587, 1063
PciSI GCTCTTC 1 cut(s) 1165
PctI GAATGC 1 cut(s) 733
PdiI GCCGGC 1 cut(s) 971
PfeI GAWTC 3 cut(s) 320, 761, 1041
PflFI GACNNNGTC 1 cut(s) 1457
PkrI GCNGC 6 cut(s) 79, 198, 472, 571, 721, 853
PleI GAGTC 2 cut(s) 140, 575
PpsI GAGTC 2 cut(s) 140, 575
PscI ACATGT 2 cut(s) 587, 1063
PsiI TTATAA 1 cut(s) 1139
Psp124BI GAGCTC 2 cut(s) 784, 1104
Psp1406I AACGTT 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 476
PspEI GGTNACC 1 cut(s) 499
PspFI CCCAGC 2 cut(s) 199, 1423
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 3 cut(s) 176, 606, 924
PspPI GGNCC 2 cut(s) 711, 1286
PstNI CAGNNNCTG 1 cut(s) 1495
PsyI GACNNNGTC 1 cut(s) 1457
PvuII CAGCTG 2 cut(s) 199, 821
RsaI GTAC 4 cut(s) 592, 831, 924, 1502
RsaNI GTAC 4 cut(s) 591, 830, 923, 1501
RseI CAYNNNNRTG 2 cut(s) 464, 916
SacI GAGCTC 2 cut(s) 784, 1104
SacII CCGCGG 2 cut(s) 81, 573
SapI GCTCTTC 1 cut(s) 1165
SaqAI TTAA 9 cut(s) 108, 134, 453, 684, 756, 879, 1227, 1358, 1376
SatI GCNGC 6 cut(s) 78, 197, 471, 570, 720, 852
Sau3AI GATC 7 cut(s) 120, 165, 397, 433, 738, 897, 1336
Sau96I GGNCC 2 cut(s) 711, 1286
ScaI AGTACT 1 cut(s) 1502
SchI GAGTC 2 cut(s) 140, 575
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 2 cut(s) 784, 1104
SfaNI GCATC 8 cut(s) 75, 304, 347, 369, 739, 808, 863, 1111
SfcI CTRYAG 2 cut(s) 289, 843
Sfr303I CCGCGG 2 cut(s) 81, 573
SgrAI CRCCGGYG 1 cut(s) 969
SgrBI CCGCGG 2 cut(s) 81, 573
SmiMI CAYNNNNRTG 2 cut(s) 464, 916
SmlI CTYRAG 4 cut(s) 133, 418, 452, 683
SmoI CTYRAG 4 cut(s) 133, 418, 452, 683
Sse9I AATT 3 cut(s) 239, 347, 508
SsiI CCGC 5 cut(s) 78, 80, 570, 572, 967
SspMI CTAG 6 cut(s) 192, 303, 522, 542, 566, 1257
SstI GAGCTC 2 cut(s) 784, 1104
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 2 cut(s) 33, 1278
TaaI ACNGT 4 cut(s) 293, 679, 943, 1018
TaiI ACGT 1 cut(s) 539
TaqI TCGA 8 cut(s) 168, 584, 741, 1044, 1180, 1350, 1394, 1472
TasI AATT 3 cut(s) 239, 347, 508
TatI WGTACW 2 cut(s) 590, 1500
TauI GCSGC 2 cut(s) 80, 572
TfiI GAWTC 3 cut(s) 320, 761, 1041
Tru1I TTAA 9 cut(s) 108, 134, 453, 684, 756, 879, 1227, 1358, 1376
Tru9I TTAA 9 cut(s) 108, 134, 453, 684, 756, 879, 1227, 1358, 1376
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 3 cut(s) 499, 868, 1464
TseI GCWGC 4 cut(s) 196, 470, 719, 851
Tsp45I GTSAC 3 cut(s) 499, 868, 1464
TspDTI ATGAA 9 cut(s) 27, 58, 149, 371, 566, 634, 1065, 1359, 1416
TspRI CASTG 1 cut(s) 682
Tth111I GACNNNGTC 1 cut(s) 1457
Vha464I CTTAAG 3 cut(s) 133, 452, 683
XapI RAATTY 2 cut(s) 347, 508
XceI RCATGY 3 cut(s) 591, 811, 1067
XspI CTAG 6 cut(s) 192, 303, 522, 542, 566, 1257
ZrmI AGTACT 1 cut(s) 1502
Zsp2I ATGCAT 2 cut(s) 90, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.