Rh6AG313000

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0007046
Physical Location & Seq
Forward (+)
51221495 .. 51223519
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG313000.1

Sequence Viewer

Length: 1155 bp
ATGCAGACAGAAGAAAACAACGGCAAAACCACCTTCGCACATATCCTATCAGCCGCTGCCATCGGCATCATCATTGCCGCGGCGGTGCATTATCGTCAACGAAAGACAAAGTATGAGAAGATCATCCCACGCTTAAGGGTCTCGGACTCCAGCCGCGTTCATAAGATCGAAAGGTTCCCTCATTATGTAGCTAGGCAGCTGGGATTTAAAGATAGAAGAGAATGTCCACACCTCTGCAAATTGGCCACCGAATACATGAGAAAATGCGACGGGTTTGAGGATGATATCTACAGTTTCTTTTCTAGTGAACCGGATGCAGACTCGCTGTTTGTGAAGCTTCTGGAGGAGTTTGAGAGATGCATCCTCAGTTATTTCGCATTTCATTGGAACGAAGCTGATCTGATGATTAGTCAGGTCTTGAGTTGTGATACTGAGCCGAGGAAGAAGCTCAAGAACATTGTCATGGCAGCGACCAGGGAACAGAGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTGGAGGAGATGAAAGCAATGGGACTGGCCACAGCGGATGACTCAACATGCACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTTATGGGAGGTGGAATGGGAGCAGGGAAGAGCACCGTGCTAAAGGACATTCTCAAAGAGCCATTCTGGGCAGGAGCAGCAGGGAATGCAGTAGTGATTGAAGCAGATGCCTTCAAAGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAATCATCTACAGACGCAGCATCATCCCTTCTGGTCACAGCACTTAACGAAGGGCGAGATGTGATCATGGATGGCACACTCTCTTGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGCCGTTATCGCATGGGAGCTGGTTACAGGGTGAATGACGATGGAACTGTCACTGAAAACTATTGGGAACGAATTGAAGATGAAGAACCAGAACAAGTAGAAGGGGGCAGAAAGAGAAAACCATACAGGATAGAGCTTGTTGGAGTTGTGTGTGATGCATATCTGGCTGTTATAAGAGGAATAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

384

Amino Acids

43.46

Weight (kDa)

6.84

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 205 - 308 1.4e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
Acc65I GGTACC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 919
AccII CGCG 2 cut(s) 80, 156
AciI CCGC 8 cut(s) 54, 78, 80, 83, 154, 572, 964, 967
AclI AACGTT 1 cut(s) 533
AcoI YGGCCR 3 cut(s) 243, 564, 948
AcsI RAATTY 1 cut(s) 505
AfaI GTAC 2 cut(s) 828, 921
AfiI CCNNNNNNNGG 1 cut(s) 643
AflII CTTAAG 1 cut(s) 133
AgsI TTSAA 6 cut(s) 491, 511, 740, 754, 812, 1043
AjnI CCWGG 1 cut(s) 473
AleI CACNNNNGTG 1 cut(s) 593
Alw21I GWGCWC 2 cut(s) 674, 781
Alw26I GTCTC 1 cut(s) 145
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 3 cut(s) 243, 564, 948
ApeKI GCWGC 5 cut(s) 56, 196, 467, 716, 848
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 4 cut(s) 311, 343, 490, 522
Asp718I GGTACC 1 cut(s) 919
AsuHPI GGTGA 3 cut(s) 508, 607, 1009
BalI TGGCCA 3 cut(s) 245, 566, 950
BanI GGYRCC 1 cut(s) 919
BanII GRGCYC 1 cut(s) 781
Bbv12I GWGCWC 2 cut(s) 674, 781
BbvI GCAGC 5 cut(s) 43, 208, 479, 728, 860
BccI CCATC 4 cut(s) 68, 592, 896, 1001
BceAI ACGGC 2 cut(s) 37, 954
BciT130I CCWGG 1 cut(s) 475
BclI TGATCA 1 cut(s) 894
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 3 cut(s) 192, 303, 519
BfmI CTRYAG 2 cut(s) 289, 840
BfrI CTTAAG 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 475
BmiI GGNNCC 3 cut(s) 176, 603, 921
BmrFI CCNGG 1 cut(s) 475
BmsI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
BpmI CTGGAG 4 cut(s) 133, 362, 560, 772
BpuEI CTTGAG 2 cut(s) 434, 439
BsaBI GATNNNNATC 1 cut(s) 1125
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 78, 437, 474
BsaWI WCCGGW 2 cut(s) 310, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse1I ACTGG 4 cut(s) 543, 567, 629, 828
Bse3DI GCAATG 2 cut(s) 72, 561
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 3 cut(s) 78, 437, 474
BseGI GGATG 8 cut(s) 123, 286, 319, 360, 580, 769, 854, 907
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMI GCAATG 2 cut(s) 72, 561
BseMII CTCAG 3 cut(s) 379, 423, 796
BseNI ACTGG 4 cut(s) 543, 567, 629, 828
BseRI GAGGAG 3 cut(s) 359, 557, 626
BseXI GCAGC 5 cut(s) 43, 208, 479, 728, 860
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 2 cut(s) 80, 156
BshFI GGCC 3 cut(s) 245, 566, 950
BshNI GGYRCC 1 cut(s) 919
BsiHKAI GWGCWC 2 cut(s) 674, 781
BsiSI CCGG 2 cut(s) 311, 605
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 3 cut(s) 245, 566, 950
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 674, 781
Bsp143I GATC 4 cut(s) 120, 165, 397, 894
BspACI CCGC 8 cut(s) 54, 78, 80, 83, 154, 572, 964, 967
BspANI GGCC 3 cut(s) 245, 566, 950
BspCNI CTCAG 3 cut(s) 378, 424, 795
BspFNI CGCG 2 cut(s) 80, 156
BspLI GGNNCC 3 cut(s) 176, 603, 921
BspQI GCTCTTC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 72, 561
BsrI ACTGG 4 cut(s) 543, 567, 629, 828
BssECI CCNNGG 3 cut(s) 78, 437, 474
BssMI GATC 4 cut(s) 120, 165, 397, 894
Bst2UI CCWGG 1 cut(s) 475
Bst4CI ACNGT 4 cut(s) 293, 676, 940, 1015
Bst6I CTCTTC 2 cut(s) 211, 662
BstAFI CTTAAG 1 cut(s) 133
BstAPI GCANNNNNTGC 1 cut(s) 725
BstDEI CTNAG 3 cut(s) 365, 432, 782
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 496
BstF5I GGATG 8 cut(s) 123, 286, 319, 360, 580, 769, 854, 907
BstFNI CGCG 2 cut(s) 80, 156
BstKTI GATC 4 cut(s) 123, 168, 400, 897
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 4 cut(s) 120, 165, 397, 894
BstMWI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
BstNI CCWGG 1 cut(s) 475
BstNSI RCATGY 2 cut(s) 588, 808
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 1 cut(s) 473
BstSFI CTRYAG 2 cut(s) 289, 840
BstUI CGCG 2 cut(s) 80, 156
BstV1I GCAGC 5 cut(s) 43, 208, 479, 728, 860
BsuRI GGCC 3 cut(s) 245, 566, 950
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 8 cut(s) 123, 286, 319, 360, 580, 769, 854, 907
BtsIMutI CAGTG 1 cut(s) 1017
CaiI CAGNNNCTG 1 cut(s) 56
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 1 cut(s) 854
Csp6I GTAC 2 cut(s) 827, 920
CviAII CATG 7 cut(s) 256, 463, 585, 614, 805, 898, 979
CviQI GTAC 2 cut(s) 827, 920
DdeI CTNAG 3 cut(s) 365, 432, 782
DpnI GATC 4 cut(s) 122, 167, 399, 896
DpnII GATC 4 cut(s) 120, 165, 397, 894
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 3 cut(s) 243, 564, 948
Eam1104I CTCTTC 2 cut(s) 211, 662
EarI CTCTTC 2 cut(s) 211, 662
Ecl136II GAGCTC 1 cut(s) 779
Eco24I GRGCYC 1 cut(s) 781
Eco31I GGTCTC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 779
Eco91I GGTNACC 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 779
EcoO65I GGTNACC 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 473
EcoRV GATATC 1 cut(s) 286
EcoT22I ATGCAT 2 cut(s) 362, 1126
EcoT38I GRGCYC 1 cut(s) 781
FaeI CATG 7 cut(s) 259, 466, 588, 617, 808, 901, 982
FaqI GGGAC 1 cut(s) 573
FatI CATG 7 cut(s) 255, 462, 584, 613, 804, 897, 978
FbaI TGATCA 1 cut(s) 894
FokI GGATG 8 cut(s) 110, 293, 326, 347, 587, 776, 841, 914
FriOI GRGCYC 1 cut(s) 781
FspBI CTAG 3 cut(s) 192, 303, 519
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 4 cut(s) 133, 362, 560, 772
HaeIII GGCC 3 cut(s) 245, 566, 950
HapII CCGG 2 cut(s) 311, 605
HgaI GACGC 1 cut(s) 854
Hin1II CATG 7 cut(s) 259, 466, 588, 617, 808, 901, 982
HincII GTYRAC 1 cut(s) 98
HindII GTYRAC 1 cut(s) 98
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 4 cut(s) 146, 320, 578, 758
HpaII CCGG 2 cut(s) 311, 605
HphI GGTGA 3 cut(s) 508, 607, 1009
Hpy166II GTNNAC 5 cut(s) 98, 227, 308, 829, 961
Hpy188I TCNGA 4 cut(s) 145, 402, 763, 936
Hpy188III TCNNGA 5 cut(s) 341, 418, 451, 789, 800
Hpy8I GTNNAC 5 cut(s) 98, 227, 308, 829, 961
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 6 cut(s) 43, 505, 760, 869, 875, 1061
HpyCH4III ACNGT 4 cut(s) 293, 676, 940, 1015
HpyCH4IV ACGT 1 cut(s) 533
HpyCH4V TGCA 8 cut(s) 4, 88, 237, 317, 360, 588, 728, 1124
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
HpyF3I CTNAG 3 cut(s) 365, 432, 782
HpySE526I ACGT 1 cut(s) 533
Hsp92II CATG 7 cut(s) 259, 466, 588, 617, 808, 901, 982
KpnI GGTACC 1 cut(s) 923
Ksp22I TGATCA 1 cut(s) 894
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 4 cut(s) 120, 165, 397, 894
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 5 cut(s) 607, 659, 713, 791, 983
Lsp1109I GCAGC 5 cut(s) 43, 208, 479, 728, 860
LweI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
MaeI CTAG 3 cut(s) 192, 303, 519
MaeII ACGT 1 cut(s) 533
MaeIII GTNAC 6 cut(s) 496, 534, 865, 940, 989, 1015
MalI GATC 4 cut(s) 122, 167, 399, 896
MboI GATC 4 cut(s) 120, 165, 397, 894
MboII GAAGA 7 cut(s) 23, 130, 228, 454, 679, 1055, 1061
MhlI GDGCHC 2 cut(s) 674, 781
MlsI TGGCCA 3 cut(s) 245, 566, 950
MluCI AATT 3 cut(s) 239, 505, 1038
MluNI TGGCCA 3 cut(s) 245, 566, 950
MlyI GAGTC 3 cut(s) 140, 314, 572
MmeI TCCRAC 1 cut(s) 1087
Mox20I TGGCCA 3 cut(s) 245, 566, 950
Mph1103I ATGCAT 2 cut(s) 362, 1126
MscI TGGCCA 3 cut(s) 245, 566, 950
MseI TTAA 3 cut(s) 134, 207, 876
MslI CAYNNNNRTG 2 cut(s) 461, 593
Msp20I TGGCCA 3 cut(s) 245, 566, 950
MspA1I CMGCKG 5 cut(s) 56, 80, 199, 572, 818
MspCI CTTAAG 1 cut(s) 133
MspI CCGG 2 cut(s) 311, 605
MspR9I CCNGG 1 cut(s) 475
Mva1269I GAATGC 1 cut(s) 730
MvaI CCWGG 1 cut(s) 475
MvnI CGCG 2 cut(s) 80, 156
MwoI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
NdeII GATC 4 cut(s) 120, 165, 397, 894
NlaIII CATG 7 cut(s) 259, 466, 588, 617, 808, 901, 982
NlaIV GGNNCC 3 cut(s) 176, 603, 921
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 3 cut(s) 496, 865, 1015
NsiI ATGCAT 2 cut(s) 362, 1126
NspI RCATGY 2 cut(s) 588, 808
OliI CACNNNNGTG 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 662
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 1 cut(s) 758
PleI GAGTC 3 cut(s) 140, 314, 572
PpsI GAGTC 3 cut(s) 140, 314, 572
PsiI TTATAA 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 781
Psp1406I AACGTT 1 cut(s) 533
Psp6I CCWGG 1 cut(s) 473
PspEI GGTNACC 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 473
PspN4I GGNNCC 3 cut(s) 176, 603, 921
PstNI CAGNNNCTG 1 cut(s) 56
PvuII CAGCTG 2 cut(s) 199, 818
RsaI GTAC 2 cut(s) 828, 921
RsaNI GTAC 2 cut(s) 827, 920
RseI CAYNNNNRTG 2 cut(s) 461, 593
SacI GAGCTC 1 cut(s) 781
SacII CCGCGG 1 cut(s) 81
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 3 cut(s) 134, 207, 876
Sau3AI GATC 4 cut(s) 120, 165, 397, 894
SchI GAGTC 3 cut(s) 140, 314, 572
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 2 cut(s) 674, 781
SfaNI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
SfcI CTRYAG 2 cut(s) 289, 840
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 461, 593
SmlI CTYRAG 3 cut(s) 133, 418, 449
SmoI CTYRAG 3 cut(s) 133, 418, 449
Sse9I AATT 3 cut(s) 239, 505, 1038
SsiI CCGC 8 cut(s) 54, 78, 80, 83, 154, 572, 964, 967
SspMI CTAG 3 cut(s) 192, 303, 519
SstI GAGCTC 1 cut(s) 781
StyD4I CCNGG 1 cut(s) 473
TaaI ACNGT 4 cut(s) 293, 676, 940, 1015
TaiI ACGT 1 cut(s) 536
TaqI TCGA 1 cut(s) 168
TasI AATT 3 cut(s) 239, 505, 1038
TauI GCSGC 5 cut(s) 56, 80, 83, 156, 969
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 3 cut(s) 134, 207, 876
Tru9I TTAA 3 cut(s) 134, 207, 876
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 3 cut(s) 496, 865, 1015
TseI GCWGC 5 cut(s) 56, 196, 467, 716, 848
Tsp45I GTSAC 3 cut(s) 496, 865, 1015
TspDTI ATGAA 4 cut(s) 149, 371, 563, 1062
TspRI CASTG 1 cut(s) 1024
Vha464I CTTAAG 1 cut(s) 133
XapI RAATTY 1 cut(s) 505
XceI RCATGY 2 cut(s) 588, 808
XspI CTAG 3 cut(s) 192, 303, 519
Zsp2I ATGCAT 2 cut(s) 362, 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.