AT1G09550

pectin acetylesterase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005575
Physical Location & Seq
Reverse (-)
3089576 .. 3092327
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09550.1

Sequence Viewer

Length: 1167 bp
ATGAAAACGCTTTTGTATTGGGGTTGGAGTAGTCTCGCAGGACTCATTCTCTTCAGCATTCTTGCACATGGGGAAATGAAAACCTTCAACGAGTCCAATGGAACAAATGCAAATGTCCTGATGGTAGGACTCACGCTCGTCCAAGCCGCTGCTGCTAAAGGAGCGGTGTGTCTGGATGGGTCAGTGCCTGGATATCATTTGTGTCGCGGGTATGGTTCAGGAGCAAACAACTGGATCATTCAGTTGCAGGGAGGTGCATGGTGTGACAGCATACAAAACTGTCAGAGCCGTAAAGGGAGTGGTTATGGATCATCTACCTTAATGGAGAAAGAGTTGGCATTTTTAGGATTACTAAGCAATAAAGCCGCTGAAAATCCAGACTTTTACAATTGGAACAAAGTAAAAGTCCGGTATTGTGATGGTGCATCCTTTGATGGTGACAGCGAAAACAAGGCGGCACAACTTCAGTATAGAGGAAAGCGTATATTTTTAGCTGTCATGGAAGATTTGATGGAAAAGGGAATGCGTCAGGCAAAACAGGCTTTGCTCAGCGGATGTTCTTCTGGGGGTCTTTCTGCCATTTTACGATGCGATGATTTCAATAACTTGTTTCCTCCTACCACCACAGTAAAATGCATGAGCGATGCTGGCTTCTTCCTCGACGCCGTTGATGTATCTGGAGGTCATTCTTTAAGGCGTATGTATTCTGGTGTTGTGAACACTCAGGGTTTGCAAAACACACTTCCTCCCACTTGTACAAGCCATATCAAACCATTTTTGTGTTTTTTCCCTCAATACATAATCAACCAAGTCAAGACCCCGTTGTTTATTCTCAACTCGGGATTTGATTCGTGGCAGATTGGAAATAGTTTAGCCCCACCATCTGCTGATAAGAGTGGCAGTTGGCATAACTGCAGTTTTAGCTTTAGGTGCACTGCCTCTCAGATGCACTTCTTAGAAGGTTTCAAGATGAGCATGTTAGATGCGTTGAAGACCTTTTCGAAGTTTAGCAAGAATGGAGTTTTAATTACCTCGGGATGGGCTCATTGTCAAGCGGAGAGACAAGATACTTGGTTTCCTGGATACTCTGGAGCGGGCAAAGCTAAGGGGATTGCTGTGGCTGTTGGAGATTGGTATTTCGAAAGAACAAAACAGAATAGCTCATAG

Protein Analysis

388

Amino Acids

42.34

Weight (kDa)

8.71

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 37 - 385 7.4e-150 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 164, 1094
AccII CGCG 1 cut(s) 207
AciI CCGC 8 cut(s) 147, 164, 207, 366, 455, 552, 1055, 1094
AclWI GGATC 2 cut(s) 242, 316
AcuI CTGAAG 2 cut(s) 37, 449
AcyI GRCGYC 1 cut(s) 663
AfaI GTAC 1 cut(s) 757
AfiI CCNNNNNNNGG 1 cut(s) 1038
AgsI TTSAA 4 cut(s) 88, 601, 967, 991
AjnI CCWGG 2 cut(s) 187, 1078
AluBI AGCT 4 cut(s) 494, 924, 1103, 1161
AluI AGCT 4 cut(s) 494, 924, 1103, 1161
Alw21I GWGCWC 1 cut(s) 935
Alw26I GTCTC 2 cut(s) 38, 1054
Alw44I GTGCAC 1 cut(s) 931
AlwI GGATC 2 cut(s) 242, 316
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 838, 1033
ApaLI GTGCAC 1 cut(s) 931
ApeKI GCWGC 2 cut(s) 149, 152
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
Asp700I GAANNNNTTC 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 449
AsuII TTCGAA 2 cut(s) 1001, 1140
AvaI CYCGRG 2 cut(s) 838, 1033
BaeGI GKGCMC 1 cut(s) 935
BanII GRGCYC 1 cut(s) 1045
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 935
BbvI GCAGC 2 cut(s) 136, 139
BccI CCATC 7 cut(s) 115, 170, 413, 428, 505, 889, 1032
BceAI ACGGC 2 cut(s) 273, 650
BciT130I CCWGG 2 cut(s) 189, 1080
BciVI GTATCC 1 cut(s) 1076
BcoDI GTCTC 2 cut(s) 38, 1054
BfmI CTRYAG 1 cut(s) 913
BfuI GTATCC 1 cut(s) 1076
BisI GCNGC 5 cut(s) 147, 150, 153, 366, 456
BlpI GCTNAGC 1 cut(s) 548
BlsI GCNGC 5 cut(s) 148, 151, 154, 367, 457
Bme1390I CCNGG 2 cut(s) 189, 1080
BmeT110I CYCGRG 2 cut(s) 838, 1033
BmrFI CCNGG 2 cut(s) 189, 1080
BmsI GCATC 5 cut(s) 434, 578, 634, 936, 973
BpiI GAAGAC 1 cut(s) 998
BpmI CTGGAG 2 cut(s) 699, 1110
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu1102I GCTNAGC 1 cut(s) 548
Bpu14I TTCGAA 2 cut(s) 1001, 1140
BsaHI GRCGYC 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 1032
BsaWI WCCGGW 1 cut(s) 408
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 730, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 1038
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 189, 1080
BseDI CCNNGG 1 cut(s) 1032
BseGI GGATG 4 cut(s) 181, 425, 560, 1043
BseLI CCNNNNNNNGG 1 cut(s) 1038
BseMII CTCAG 3 cut(s) 562, 737, 956
BseNI ACTGG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 935
BseXI GCAGC 2 cut(s) 136, 139
Bsh1236I CGCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 935
BsiHKCI CYCGRG 2 cut(s) 838, 1033
BsiSI CCGG 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 1038
BsmAI GTCTC 2 cut(s) 38, 1054
BsmI GAATGC 2 cut(s) 57, 528
BsoBI CYCGRG 2 cut(s) 838, 1033
Bsp119I TTCGAA 2 cut(s) 1001, 1140
Bsp1286I GDGCHC 2 cut(s) 935, 1045
Bsp1407I TGTACA 1 cut(s) 755
Bsp143I GATC 2 cut(s) 234, 308
Bsp1720I GCTNAGC 1 cut(s) 548
BspACI CCGC 8 cut(s) 147, 164, 207, 366, 455, 552, 1055, 1094
BspCNI CTCAG 3 cut(s) 561, 736, 955
BspFNI CGCG 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 917
BspPI GGATC 2 cut(s) 242, 316
BspT104I TTCGAA 2 cut(s) 1001, 1140
BsrBI CCGCTC 2 cut(s) 164, 1094
BsrGI TGTACA 1 cut(s) 755
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 1032
BssMI GATC 2 cut(s) 234, 308
BssNI GRCGYC 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 189, 1080
Bst4CI ACNGT 2 cut(s) 281, 628
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 755
BstBI TTCGAA 2 cut(s) 1001, 1140
BstC8I GCNNGC 2 cut(s) 649, 1096
BstDEI CTNAG 6 cut(s) 353, 548, 723, 942, 955, 1104
BstF5I GGATG 4 cut(s) 181, 425, 560, 1043
BstFNI CGCG 1 cut(s) 207
BstKTI GATC 2 cut(s) 237, 311
BstMAI GTCTC 2 cut(s) 38, 1054
BstMBI GATC 2 cut(s) 234, 308
BstMWI GCNNNNNNNGC 7 cut(s) 152, 161, 539, 648, 921, 930, 1100
BstNI CCWGG 2 cut(s) 189, 1080
BstNSI RCATGY 1 cut(s) 979
BstSCI CCNGG 2 cut(s) 187, 1078
BstSFI CTRYAG 1 cut(s) 913
BstSLI GKGCMC 1 cut(s) 935
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 136, 139
BstV2I GAAGAC 1 cut(s) 998
BsuI GTATCC 1 cut(s) 1076
BtgZI GCGATG 2 cut(s) 606, 657
BtsCI GGATG 4 cut(s) 181, 425, 560, 1043
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 2 cut(s) 189, 933
Cac8I GCNNGC 2 cut(s) 649, 1096
CaiI CAGNNNCTG 1 cut(s) 188
CseI GACGC 2 cut(s) 515, 671
Csp6I GTAC 1 cut(s) 756
CviAII CATG 5 cut(s) 68, 258, 499, 637, 976
CviQI GTAC 1 cut(s) 756
DdeI CTNAG 6 cut(s) 353, 548, 723, 942, 955, 1104
DpnI GATC 2 cut(s) 236, 310
DpnII GATC 2 cut(s) 234, 308
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 1045
Eco32I GATATC 1 cut(s) 194
Eco57I CTGAAG 2 cut(s) 37, 449
Eco88I CYCGRG 2 cut(s) 838, 1033
EcoRII CCWGG 2 cut(s) 187, 1078
EcoRV GATATC 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 638
EcoT38I GRGCYC 1 cut(s) 1045
FaeI CATG 5 cut(s) 71, 261, 502, 640, 979
FatI CATG 5 cut(s) 67, 257, 498, 636, 975
FauI CCCGC 2 cut(s) 200, 1087
Fnu4HI GCNGC 5 cut(s) 147, 150, 153, 366, 456
FokI GGATG 4 cut(s) 188, 412, 567, 1050
FriOI GRGCYC 1 cut(s) 1045
Fsp4HI GCNGC 5 cut(s) 147, 150, 153, 366, 456
GluI GCNGC 5 cut(s) 147, 150, 153, 366, 456
GsuI CTGGAG 2 cut(s) 699, 1110
HapII CCGG 1 cut(s) 409
HgaI GACGC 2 cut(s) 515, 671
Hin1I GRCGYC 1 cut(s) 663
Hin1II CATG 5 cut(s) 71, 261, 502, 640, 979
HinfI GANTC 4 cut(s) 42, 92, 129, 848
HpaII CCGG 1 cut(s) 409
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 718, 933
Hpy188I TCNGA 2 cut(s) 285, 945
Hpy8I GTNNAC 2 cut(s) 718, 933
Hpy99I CGWCG 1 cut(s) 665
HpyAV CCTTC 2 cut(s) 94, 953
HpyCH4III ACNGT 2 cut(s) 281, 628
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 161, 539, 648, 921, 930, 1100
HpyF3I CTNAG 6 cut(s) 353, 548, 723, 942, 955, 1104
Hsp92I GRCGYC 1 cut(s) 663
Hsp92II CATG 5 cut(s) 71, 261, 502, 640, 979
Kzo9I GATC 2 cut(s) 234, 308
LmnI GCTCC 3 cut(s) 161, 221, 1091
Lsp1109I GCAGC 2 cut(s) 136, 139
LweI GCATC 5 cut(s) 434, 578, 634, 936, 973
MaeIII GTNAC 2 cut(s) 263, 437
MalI GATC 2 cut(s) 236, 310
MbiI CCGCTC 2 cut(s) 164, 1094
MboI GATC 2 cut(s) 234, 308
MboII GAAGA 5 cut(s) 43, 515, 552, 646, 1003
MfeI CAATTG 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 935, 1045
MluCI AATT 2 cut(s) 388, 1026
MlyI GAGTC 3 cut(s) 36, 101, 123
MmeI TCCRAC 2 cut(s) 5, 1105
MnlI CCTC 9 cut(s) 245, 467, 624, 668, 674, 756, 801, 949, 1042
Mph1103I ATGCAT 1 cut(s) 638
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 3 cut(s) 320, 692, 1025
MslI CAYNNNNRTG 1 cut(s) 778
MspA1I CMGCKG 3 cut(s) 149, 368, 552
MspI CCGG 1 cut(s) 409
MspR9I CCNGG 2 cut(s) 189, 1080
MunI CAATTG 1 cut(s) 388
Mva1269I GAATGC 2 cut(s) 57, 528
MvaI CCWGG 2 cut(s) 189, 1080
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 7 cut(s) 152, 161, 539, 648, 921, 930, 1100
NdeII GATC 2 cut(s) 234, 308
NlaIII CATG 5 cut(s) 71, 261, 502, 640, 979
NmuCI GTSAC 2 cut(s) 263, 437
NsiI ATGCAT 1 cut(s) 638
NspI RCATGY 1 cut(s) 979
NspV TTCGAA 2 cut(s) 1001, 1140
PctI GAATGC 2 cut(s) 57, 528
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 848
PfoI TCCNGGA 1 cut(s) 1078
PkrI GCNGC 5 cut(s) 148, 151, 154, 367, 457
PleI GAGTC 3 cut(s) 36, 100, 123
PpsI GAGTC 3 cut(s) 36, 100, 123
Psp6I CCWGG 2 cut(s) 187, 1078
PspGI CCWGG 2 cut(s) 187, 1078
PstI CTGCAG 1 cut(s) 917
PstNI CAGNNNCTG 1 cut(s) 188
RsaI GTAC 1 cut(s) 757
RsaNI GTAC 1 cut(s) 756
RseI CAYNNNNRTG 1 cut(s) 778
SaqAI TTAA 3 cut(s) 320, 692, 1025
SatI GCNGC 5 cut(s) 147, 150, 153, 366, 456
Sau3AI GATC 2 cut(s) 234, 308
SchI GAGTC 3 cut(s) 36, 101, 123
ScrFI CCNGG 2 cut(s) 189, 1080
SduI GDGCHC 2 cut(s) 935, 1045
SfaNI GCATC 5 cut(s) 434, 578, 634, 936, 973
SfcI CTRYAG 1 cut(s) 913
SfuI TTCGAA 2 cut(s) 1001, 1140
SmiMI CAYNNNNRTG 1 cut(s) 778
Sse9I AATT 2 cut(s) 388, 1026
SsiI CCGC 8 cut(s) 147, 164, 207, 366, 455, 552, 1055, 1094
StyD4I CCNGG 2 cut(s) 187, 1078
TaaI ACNGT 2 cut(s) 281, 628
TaqI TCGA 3 cut(s) 660, 1001, 1140
TasI AATT 2 cut(s) 388, 1026
TatI WGTACW 1 cut(s) 755
TauI GCSGC 3 cut(s) 149, 368, 458
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 3 cut(s) 320, 692, 1025
Tru9I TTAA 3 cut(s) 320, 692, 1025
TscAI CASTG 2 cut(s) 189, 940
TseFI GTSAC 2 cut(s) 263, 437
TseI GCWGC 2 cut(s) 149, 152
Tsp45I GTSAC 2 cut(s) 263, 437
TspDTI ATGAA 2 cut(s) 17, 92
TspRI CASTG 2 cut(s) 189, 940
VneI GTGCAC 1 cut(s) 931
XceI RCATGY 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 83
Zsp2I ATGCAT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.