MD13G1136800.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005575
Physical Location & Seq
Forward (+)
10503438 .. 10506026
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1136800.v1.1.491

Sequence Viewer

Length: 882 bp
ATGAGATTTCCGGGGTCAGCCAGCATGAAGCTTTTCTGGGTTTTGGGGTTTATGGGGTTTGTGTTTTGCAGAAGAACAGATGGAACTCTTATTGGGGATGTGAATGTCAAACAGGAACAAGACCTGCATTATACTGAGAGTAATGTGTATGGCTATGCAACTTATGGTTATGGGGTTTCCAGCAGACCCTTAATGGTGGGTCTCACACTCATCAATGGAGCTGCTGCCAAAGGAGCAGTCTGCCTAGATGGAACATTACCTGCTTACCACATCCACCACGGATACGGGTCAGGAGCAAACAGTTGGCTCATTCAGTTAGAGGGAGGAGGATGGTGTGATACAATCAGAAACTGTGTCTACCGTAAGAAAACTCATCGCGGTTCATCAGCATATATGGAGAAGCATATACCATTTGCAGGGATACTAAGCAACAAAGCAGGAGAAAACCCTGACTTTTACAATTGGAACAGAGTTAAGGTTCGGTATTGTGATGGTGCATCTTTTGCCGGTGACAGTCACAATGAGGCTGCCCAATTGCATTTTCGAGGACAACGTATCTGGAAAGCTGCTATGGAAGACTTGATGTCAAAGGGAATGCGCTATGCTAATCAGGCTCTTCTTTCTGGATGTTCTGCTGGTGGGGTTGCAACTGTACTCCACTGTGATGAATTTCGTGGAATGTTCCCTGCAACTACTAGAGTGAAGTGCCTCAGTGATGCTGGACTGTTTCTTGACGTAGTTGATGTATCTGGTAGTCGCACTTTGAGAAGTATGTTTAGAAGAGTGGTTAGTCTACAGGGGGTGCAAAAGAATCTCCCATGGAGTTGTACCAATCGCCTCCACCCGACTTTGGTAGGTTCTTTTCTTGCATTTAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

294

Amino Acids

32.29

Weight (kDa)

9.22

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 61 - 283 8.4e-107 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 132, 268
AccI GTMKAC 2 cut(s) 357, 793
AccII CGCG 1 cut(s) 378
AciI CCGC 1 cut(s) 378
AcsI RAATTY 1 cut(s) 668
AfaI GTAC 2 cut(s) 654, 829
AfiI CCNNNNNNNGG 2 cut(s) 416, 850
AhdI GACNNNNNGTC 1 cut(s) 583
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 3 cut(s) 31, 221, 566
AluI AGCT 3 cut(s) 31, 221, 566
Alw26I GTCTC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 351
ApeKI GCWGC 4 cut(s) 221, 224, 527, 566
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 600
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 4 cut(s) 208, 211, 514, 553
BccI CCATC 4 cut(s) 74, 242, 324, 485
BciVI GTATCC 2 cut(s) 275, 414
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 245, 696
BfmI CTRYAG 1 cut(s) 794
BfuAI ACCTGC 2 cut(s) 132, 268
BfuI GTATCC 2 cut(s) 275, 414
BisI GCNGC 4 cut(s) 222, 225, 528, 567
BlsI GCNGC 4 cut(s) 223, 226, 529, 568
Bme1390I CCNGG 1 cut(s) 12
BmeRI GACNNNNNGTC 1 cut(s) 583
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 506, 706
BpiI GAAGAC 1 cut(s) 582
BpuMI CCSGG 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 11, 277, 818
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 850
Bse118I RCCGGY 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 11, 277, 818
BseGI GGATG 4 cut(s) 103, 270, 335, 632
BseLI CCNNNNNNNGG 2 cut(s) 416, 850
BseMII CTCAG 2 cut(s) 126, 724
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 4 cut(s) 208, 211, 514, 553
Bsh1236I CGCG 1 cut(s) 378
BsiSI CCGG 2 cut(s) 11, 507
BslI CCNNNNNNNGG 2 cut(s) 416, 850
BsmAI GTCTC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 600
Bso31I GGTCTC 1 cut(s) 206
Bsp19I CCATGG 1 cut(s) 818
BspACI CCGC 1 cut(s) 378
BspCNI CTCAG 2 cut(s) 127, 723
BspFNI CGCG 1 cut(s) 378
BspMI ACCTGC 2 cut(s) 132, 268
BspQI GCTCTTC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 11, 277, 818
BssT1I CCWWGG 1 cut(s) 818
Bst4CI ACNGT 7 cut(s) 302, 353, 362, 515, 652, 662, 726
Bst6I CTCTTC 2 cut(s) 621, 775
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 22
BstDEI CTNAG 3 cut(s) 135, 425, 710
BstDSI CCRYGG 2 cut(s) 277, 818
BstF5I GGATG 4 cut(s) 103, 270, 335, 632
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 600
BstMAI GTCTC 1 cut(s) 206
BstMWI GCNNNNNNNGC 3 cut(s) 233, 503, 611
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 208, 211, 514, 553
BstV2I GAAGAC 1 cut(s) 582
BsuI GTATCC 2 cut(s) 275, 414
BtgI CCRYGG 2 cut(s) 277, 818
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 4 cut(s) 103, 270, 335, 632
BtsIMutI CAGTG 2 cut(s) 658, 718
BveI ACCTGC 2 cut(s) 132, 268
Cac8I GCNNGC 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 351
CfoI GCGC 1 cut(s) 600
Cfr10I RCCGGY 1 cut(s) 506
Csp6I GTAC 2 cut(s) 653, 828
CviAII CATG 2 cut(s) 25, 819
CviJI RGCY 8 cut(s) 20, 31, 153, 221, 307, 527, 566, 614
CviKI_1 RGCY 8 cut(s) 20, 31, 153, 221, 307, 527, 566, 614
CviQI GTAC 2 cut(s) 653, 828
DdeI CTNAG 3 cut(s) 135, 425, 710
DriI GACNNNNNGTC 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 621, 775
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 2 cut(s) 621, 775
Eco130I CCWWGG 1 cut(s) 818
Eco31I GGTCTC 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 818
ErhI CCWWGG 1 cut(s) 818
FaeI CATG 2 cut(s) 28, 822
FatI CATG 2 cut(s) 24, 818
FblI GTMKAC 2 cut(s) 357, 793
Fnu4HI GCNGC 4 cut(s) 222, 225, 528, 567
FokI GGATG 4 cut(s) 110, 257, 342, 639
Fsp4HI GCNGC 4 cut(s) 222, 225, 528, 567
FspBI CTAG 2 cut(s) 245, 696
GlaI GCGC 1 cut(s) 599
GluI GCNGC 4 cut(s) 222, 225, 528, 567
HapII CCGG 2 cut(s) 11, 507
HhaI GCGC 1 cut(s) 600
Hin1II CATG 2 cut(s) 28, 822
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 811
HpaII CCGG 2 cut(s) 11, 507
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 2 cut(s) 358, 794
Hpy188I TCNGA 1 cut(s) 347
Hpy188III TCNNGA 4 cut(s) 291, 559, 624, 731
Hpy8I GTNNAC 2 cut(s) 358, 794
HpyCH4III ACNGT 7 cut(s) 302, 353, 362, 515, 652, 662, 726
HpyCH4IV ACGT 2 cut(s) 553, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 503, 611
HpyF3I CTNAG 3 cut(s) 135, 425, 710
HpySE526I ACGT 2 cut(s) 553, 735
Hsp92II CATG 2 cut(s) 28, 822
HspAI GCGC 1 cut(s) 598
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 3 cut(s) 218, 233, 293
Lsp1109I GCAGC 4 cut(s) 208, 211, 514, 553
LweI GCATC 2 cut(s) 506, 706
MaeI CTAG 2 cut(s) 245, 696
MaeII ACGT 2 cut(s) 553, 735
MaeIII GTNAC 2 cut(s) 509, 515
MboII GAAGA 4 cut(s) 84, 587, 608, 792
MfeI CAATTG 2 cut(s) 460, 533
MluCI AATT 3 cut(s) 460, 533, 668
MnlI CCTC 7 cut(s) 313, 317, 320, 517, 539, 719, 848
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 4 cut(s) 191, 474, 873, 880
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 2 cut(s) 11, 507
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 2 cut(s) 460, 533
Mva1269I GAATGC 1 cut(s) 600
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 3 cut(s) 233, 503, 611
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 818
NlaIII CATG 2 cut(s) 28, 822
NmuCI GTSAC 2 cut(s) 509, 515
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PctI GAATGC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 811
PkrI GCNGC 4 cut(s) 223, 226, 529, 568
PstNI CAGNNNCTG 1 cut(s) 351
RsaI GTAC 2 cut(s) 654, 829
RsaNI GTAC 2 cut(s) 653, 828
RseI CAYNNNNRTG 1 cut(s) 663
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 4 cut(s) 191, 474, 873, 880
SatI GCNGC 4 cut(s) 222, 225, 528, 567
ScrFI CCNGG 1 cut(s) 12
SetI ASST 9 cut(s) 33, 126, 223, 262, 480, 556, 568, 738, 859
SfaNI GCATC 2 cut(s) 506, 706
SfcI CTRYAG 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 663
Sse9I AATT 3 cut(s) 460, 533, 668
SsiI CCGC 1 cut(s) 378
SspMI CTAG 2 cut(s) 245, 696
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 818
TaaI ACNGT 7 cut(s) 302, 353, 362, 515, 652, 662, 726
TaiI ACGT 2 cut(s) 556, 738
TaqI TCGA 1 cut(s) 544
TasI AATT 3 cut(s) 460, 533, 668
TatI WGTACW 1 cut(s) 652
TfiI GAWTC 1 cut(s) 811
Tru1I TTAA 4 cut(s) 191, 474, 873, 880
Tru9I TTAA 4 cut(s) 191, 474, 873, 880
TscAI CASTG 2 cut(s) 665, 718
TseFI GTSAC 2 cut(s) 509, 515
TseI GCWGC 4 cut(s) 221, 224, 527, 566
Tsp45I GTSAC 2 cut(s) 509, 515
TspDTI ATGAA 3 cut(s) 41, 372, 681
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 2 cut(s) 665, 718
XapI RAATTY 1 cut(s) 668
XmiI GTMKAC 2 cut(s) 357, 793
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 245, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.