Prupe.1G208600_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005575
Physical Location & Seq
Reverse (-)
22268553 .. 22273530
4978 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G208600.3

Sequence Viewer

Length: 1443 bp
ATGAAGCTGTTGTGGGTTTTGGGGTTTATGGGGATGGTGTTGTGCAGAAGGACGGATGGTTTTGCGGATGTGAATGGTACAAAAGAAGAAGACTTGCATTACACTGAGAGTAATGTTTATGGTTATGGACCTAATGGTTATGGGGTGTTCAGCAGACCCTTGATGGTGGGTCTCACTCTTATCAATGGAGCTGCTGCCAAAGGAGCAGTCTGCCTAGATGGAACATTACCCGGTTTCCACATCCACCGTGGATATGGGTCAGGAGCAAACAGTTGGCTCATTCAGTTGGAGGGAGGAGGATGGTGTGATACAATCAGAAATTGTGTCTATAGAAAGAAAACTCGTCGTGGTTCATCAGCATATATGGAGAAGCAGATACCATTTGCAGGGATATTGAGCAACAAAGCTGGAGAAAACCCTGACTTTTACAACTGGAACAGAGTCAAGGTTCGATATTGTGATGGTGCATCTTTTGGTGGTGACAGTCAAAATGAGGCAGCCCAATTGTATTTTCGAGGCCAACGAATCTGGGAAGCTGCTATGGCAGATTTGATGTCAAAGGGAATGCGCTATGCTAATCAGGCTCTTCTTTCTGGATGTTCTGCTGGTGGTGTTGCAACTGTACTCCATTGTGATGAGTTTCGTGCCATGTTTCGTGGAACTACTAGAGTCAAGTGCTTGAGTGATGCTGGATTATTTCTTGATGTAATTGATGTATCTGGTAGGCGCACATTAAGAAATATGTTTAGACGAGTGGTTAGCCTACAGGGAGCATACAAGAGTCTTCCATGGAGTTGTACCAATCGCCTCAACCCGACTTTGTGCTTTTTCCCGCAGCACTTAATTGCAAGTGTCAAGACCCCCCTTTTTCTGGTCAATGCAGCTTATGATACATGGCAGATCCAGGCCAGTTTAGCTTCACCAACAGCAGATCCTAATGGCCTTTGGCATGAATGCACAAAAAATCATGCGCGTTGTGCTGCATGGCAGATGAACTTTTTGCAAGGTTTGATTCCAATTGTGTCTTTTATGTTTTTCAATAAATCAGGTTTCCGGAATCAAATGCTCAAGGCTGTAAGTGGCTTCTCAAGGGCCAGTAAAAATGGTTTATTCATAAATTCTTGCTTTGCTCATTGCCAGACTGAGAGACAAGATACATGGTTTTCTCAAAATTCTCCCCATATTGGAAATAAGGTATGCCCTATTGTGTACTTATTATTTCATAAGTTACATAATGAACAAATTCCCTGTCATTTCTTTTTCACATTATTTTTTGTTATAGATGAGTCTGTCTGTCTGTGTGGTGTACTCATGCAATCCATTATATATCTTCAGGGGATAGCAAAATCAGTCGGAAACTGGTATTTCGATCGAGTTAATATCAAGGCAATAGATTGTCCATACCCTTGTGACAAGAGCTGCCATAATCTGGTGTTTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

481

Amino Acids

53.83

Weight (kDa)

9.05

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1429
AccII CGCG 1 cut(s) 973
AccIII TCCGGA 1 cut(s) 1053
AciI CCGC 2 cut(s) 65, 833
AclWI GGATC 2 cut(s) 895, 926
AcsI RAATTY 3 cut(s) 1117, 1171, 1242
AcuI CTGAAG 1 cut(s) 1316
AfaI GTAC 5 cut(s) 79, 624, 799, 1211, 1308
AfiI CCNNNNNNNGG 4 cut(s) 386, 871, 1184, 1429
AgsI TTSAA 1 cut(s) 1039
AjnI CCWGG 1 cut(s) 903
AjuI GAANNNNNNNTTGG 2 cut(s) 495, 527
AluBI AGCT 7 cut(s) 7, 191, 407, 536, 884, 917, 1419
AluI AGCT 7 cut(s) 7, 191, 407, 536, 884, 917, 1419
Alw26I GTCTC 2 cut(s) 176, 1141
AlwI GGATC 2 cut(s) 895, 926
Aor13HI TCCGGA 1 cut(s) 1053
AoxI GGCC 4 cut(s) 517, 906, 940, 1092
ApeKI GCWGC 8 cut(s) 191, 194, 497, 536, 835, 881, 980, 1419
ApoI RAATTY 3 cut(s) 1117, 1171, 1242
ArsI GACNNNNNNTTYG 4 cut(s) 192, 224, 1234, 1266
Asp700I GAANNNNTTC 1 cut(s) 1242
AspLEI GCGC 3 cut(s) 570, 729, 973
AspS9I GGNCC 2 cut(s) 128, 1092
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 2 cut(s) 491, 912
AvaII GGWCC 1 cut(s) 128
BbsI GAAGAC 2 cut(s) 96, 776
BbvI GCAGC 8 cut(s) 178, 181, 509, 523, 847, 893, 967, 1406
BccI CCATC 6 cut(s) 28, 50, 157, 212, 294, 455
BciT130I CCWGG 1 cut(s) 905
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 176, 1141
BfaI CTAG 2 cut(s) 215, 666
BfmI CTRYAG 2 cut(s) 328, 764
BisI GCNGC 8 cut(s) 192, 195, 498, 537, 836, 882, 981, 1420
BlsI GCNGC 8 cut(s) 193, 196, 499, 538, 837, 883, 982, 1421
Bme1390I CCNGG 2 cut(s) 231, 905
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 128, 1092
BmrFI CCNGG 2 cut(s) 231, 905
BmsI GCATC 2 cut(s) 476, 676
BpiI GAAGAC 2 cut(s) 96, 776
BpmI CTGGAG 1 cut(s) 429
BpuEI CTTGAG 3 cut(s) 700, 1052, 1072
BpuMI CCSGG 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 247, 788
BsaWI WCCGGW 1 cut(s) 1053
Bsc4I CCNNNNNNNGG 4 cut(s) 386, 871, 1184, 1429
Bse1I ACTGG 4 cut(s) 437, 909, 1095, 1364
Bse3DI GCAATG 1 cut(s) 1132
BseAI TCCGGA 1 cut(s) 1053
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 2 cut(s) 247, 788
BseGI GGATG 6 cut(s) 39, 61, 73, 240, 305, 602
BseLI CCNNNNNNNGG 4 cut(s) 386, 871, 1184, 1429
BseMI GCAATG 1 cut(s) 1132
BseMII CTCAG 2 cut(s) 96, 1134
BseNI ACTGG 4 cut(s) 437, 909, 1095, 1364
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 8 cut(s) 178, 181, 509, 523, 847, 893, 967, 1406
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 973
Bsh1285I CGRYCG 1 cut(s) 1372
BshFI GGCC 4 cut(s) 519, 908, 942, 1094
BsiEI CGRYCG 1 cut(s) 1372
BsiSI CCGG 2 cut(s) 231, 1054
BslI CCNNNNNNNGG 4 cut(s) 386, 871, 1184, 1429
BsmAI GTCTC 2 cut(s) 176, 1141
BsmI GAATGC 2 cut(s) 570, 959
BsnI GGCC 4 cut(s) 519, 908, 942, 1094
Bso31I GGTCTC 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 1053
Bsp143I GATC 3 cut(s) 900, 931, 1369
Bsp19I CCATGG 1 cut(s) 788
BspACI CCGC 2 cut(s) 65, 833
BspANI GGCC 4 cut(s) 519, 908, 942, 1094
BspCNI CTCAG 2 cut(s) 97, 1135
BspEI TCCGGA 1 cut(s) 1053
BspFNI CGCG 1 cut(s) 973
BspPI GGATC 2 cut(s) 895, 926
BspQI GCTCTTC 1 cut(s) 591
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 1132
BsrI ACTGG 4 cut(s) 437, 909, 1095, 1364
BssECI CCNNGG 2 cut(s) 247, 788
BssMI GATC 3 cut(s) 900, 931, 1369
BssT1I CCWWGG 1 cut(s) 788
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 4 cut(s) 248, 272, 485, 622
Bst6I CTCTTC 1 cut(s) 591
BstDEI CTNAG 2 cut(s) 105, 1143
BstDSI CCRYGG 2 cut(s) 247, 788
BstF5I GGATG 6 cut(s) 39, 61, 73, 240, 305, 602
BstFNI CGCG 1 cut(s) 973
BstHHI GCGC 3 cut(s) 570, 729, 973
BstKTI GATC 3 cut(s) 903, 934, 1372
BstMAI GTCTC 2 cut(s) 176, 1141
BstMBI GATC 3 cut(s) 900, 931, 1369
BstMCI CGRYCG 1 cut(s) 1372
BstMWI GCNNNNNNNGC 5 cut(s) 203, 542, 581, 914, 977
BstNI CCWGG 1 cut(s) 905
BstSCI CCNGG 2 cut(s) 229, 903
BstSFI CTRYAG 2 cut(s) 328, 764
BstUI CGCG 1 cut(s) 973
BstV1I GCAGC 8 cut(s) 178, 181, 509, 523, 847, 893, 967, 1406
BstV2I GAAGAC 2 cut(s) 96, 776
BstX2I RGATCY 2 cut(s) 900, 931
BstYI RGATCY 2 cut(s) 900, 931
BsuRI GGCC 4 cut(s) 519, 908, 942, 1094
BtgI CCRYGG 2 cut(s) 247, 788
BtsCI GGATG 6 cut(s) 39, 61, 73, 240, 305, 602
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 3 cut(s) 570, 729, 973
Cfr13I GGNCC 2 cut(s) 128, 1092
Csp6I GTAC 5 cut(s) 78, 623, 798, 1210, 1307
CviAII CATG 8 cut(s) 649, 789, 894, 950, 968, 984, 1158, 1312
CviQI GTAC 5 cut(s) 78, 623, 798, 1210, 1307
DdeI CTNAG 2 cut(s) 105, 1143
DpnI GATC 3 cut(s) 902, 933, 1371
DpnII GATC 3 cut(s) 900, 931, 1369
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 788
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 1316
EcoRII CCWGG 1 cut(s) 903
EcoT14I CCWWGG 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 788
FaeI CATG 8 cut(s) 652, 792, 897, 953, 971, 987, 1161, 1315
FatI CATG 8 cut(s) 648, 788, 893, 949, 967, 983, 1157, 1311
FauI CCCGC 1 cut(s) 840
Fnu4HI GCNGC 8 cut(s) 192, 195, 498, 537, 836, 882, 981, 1420
FokI GGATG 6 cut(s) 46, 68, 80, 227, 312, 609
Fsp4HI GCNGC 8 cut(s) 192, 195, 498, 537, 836, 882, 981, 1420
FspBI CTAG 2 cut(s) 215, 666
GlaI GCGC 3 cut(s) 569, 728, 972
GluI GCNGC 8 cut(s) 192, 195, 498, 537, 836, 882, 981, 1420
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 4 cut(s) 519, 908, 942, 1094
HapII CCGG 2 cut(s) 231, 1054
HhaI GCGC 3 cut(s) 570, 729, 973
Hin1II CATG 8 cut(s) 652, 792, 897, 953, 971, 987, 1161, 1315
Hin6I GCGC 3 cut(s) 568, 727, 971
HinP1I GCGC 3 cut(s) 568, 727, 971
HinfI GANTC 7 cut(s) 441, 525, 669, 781, 1012, 1057, 1286
HpaII CCGG 2 cut(s) 231, 1054
HphI GGTGA 2 cut(s) 491, 912
Hpy166II GTNNAC 2 cut(s) 1210, 1307
Hpy188I TCNGA 2 cut(s) 317, 1355
Hpy188III TCNNGA 5 cut(s) 261, 594, 701, 856, 1054
Hpy8I GTNNAC 2 cut(s) 1210, 1307
Hpy99I CGWCG 1 cut(s) 348
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 4 cut(s) 248, 272, 485, 622
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 542, 581, 914, 977
HpyF3I CTNAG 2 cut(s) 105, 1143
Hsp92II CATG 8 cut(s) 652, 792, 897, 953, 971, 987, 1161, 1315
HspAI GCGC 3 cut(s) 568, 727, 971
Kpn2I TCCGGA 1 cut(s) 1053
Kzo9I GATC 3 cut(s) 900, 931, 1369
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 4 cut(s) 188, 203, 263, 770
Lsp1109I GCAGC 8 cut(s) 178, 181, 509, 523, 847, 893, 967, 1406
LweI GCATC 2 cut(s) 476, 676
MaeI CTAG 2 cut(s) 215, 666
MaeIII GTNAC 3 cut(s) 479, 1227, 1409
MalI GATC 3 cut(s) 902, 933, 1371
MboI GATC 3 cut(s) 900, 931, 1369
MboII GAAGA 5 cut(s) 98, 101, 578, 776, 1322
MfeI CAATTG 2 cut(s) 503, 1017
MflI RGATCY 2 cut(s) 900, 931
MluCI AATT 8 cut(s) 319, 503, 708, 843, 1017, 1117, 1171, 1242
MlyI GAGTC 4 cut(s) 450, 678, 790, 1295
MmeI TCCRAC 2 cut(s) 267, 1333
MnlI CCTC 6 cut(s) 283, 287, 290, 487, 509, 818
MroI TCCGGA 1 cut(s) 1053
MroXI GAANNNNTTC 1 cut(s) 1242
MseI TTAA 3 cut(s) 734, 842, 1377
MslI CAYNNNNRTG 1 cut(s) 633
MspI CCGG 2 cut(s) 231, 1054
MspR9I CCNGG 2 cut(s) 231, 905
MunI CAATTG 2 cut(s) 503, 1017
Mva1269I GAATGC 2 cut(s) 570, 959
MvaI CCWGG 1 cut(s) 905
MvnI CGCG 1 cut(s) 973
MwoI GCNNNNNNNGC 5 cut(s) 203, 542, 581, 914, 977
NciI CCSGG 1 cut(s) 231
NcoI CCATGG 1 cut(s) 788
NdeII GATC 3 cut(s) 900, 931, 1369
NlaIII CATG 8 cut(s) 652, 792, 897, 953, 971, 987, 1161, 1315
NmuCI GTSAC 2 cut(s) 479, 1409
PciSI GCTCTTC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PctI GAATGC 2 cut(s) 570, 959
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 3 cut(s) 525, 1012, 1057
PflMI CCANNNNNTGG 1 cut(s) 1429
PkrI GCNGC 8 cut(s) 193, 196, 499, 538, 837, 883, 982, 1421
Ple19I CGATCG 1 cut(s) 1372
PleI GAGTC 4 cut(s) 449, 677, 789, 1294
PpsI GAGTC 4 cut(s) 449, 677, 789, 1294
Psp6I CCWGG 1 cut(s) 903
PspGI CCWGG 1 cut(s) 903
PspPI GGNCC 2 cut(s) 128, 1092
PsuI RGATCY 2 cut(s) 900, 931
PvuI CGATCG 1 cut(s) 1372
RsaI GTAC 5 cut(s) 79, 624, 799, 1211, 1308
RsaNI GTAC 5 cut(s) 78, 623, 798, 1210, 1307
RseI CAYNNNNRTG 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 3 cut(s) 734, 842, 1377
SatI GCNGC 8 cut(s) 192, 195, 498, 537, 836, 882, 981, 1420
Sau3AI GATC 3 cut(s) 900, 931, 1369
Sau96I GGNCC 2 cut(s) 128, 1092
SchI GAGTC 4 cut(s) 450, 678, 790, 1295
ScrFI CCNGG 2 cut(s) 231, 905
SfaNI GCATC 2 cut(s) 476, 676
SfcI CTRYAG 2 cut(s) 328, 764
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 3 cut(s) 679, 1067, 1087
SmoI CTYRAG 3 cut(s) 679, 1067, 1087
Sse9I AATT 8 cut(s) 319, 503, 708, 843, 1017, 1117, 1171, 1242
SsiI CCGC 2 cut(s) 65, 833
SspMI CTAG 2 cut(s) 215, 666
StyD4I CCNGG 2 cut(s) 229, 903
StyI CCWWGG 1 cut(s) 788
TaaI ACNGT 4 cut(s) 248, 272, 485, 622
TaqI TCGA 4 cut(s) 451, 514, 1368, 1372
TasI AATT 8 cut(s) 319, 503, 708, 843, 1017, 1117, 1171, 1242
TatI WGTACW 3 cut(s) 622, 1209, 1306
TfiI GAWTC 3 cut(s) 525, 1012, 1057
Tru1I TTAA 3 cut(s) 734, 842, 1377
Tru9I TTAA 3 cut(s) 734, 842, 1377
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 2 cut(s) 479, 1409
TseI GCWGC 8 cut(s) 191, 194, 497, 536, 835, 881, 980, 1419
Tsp45I GTSAC 2 cut(s) 479, 1409
TspDTI ATGAA 7 cut(s) 17, 342, 966, 1007, 1102, 1211, 1251
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 109
Van91I CCANNNNNTGG 1 cut(s) 1429
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 3 cut(s) 1117, 1171, 1242
XcmI CCANNNNNNNNNTGG 2 cut(s) 245, 251
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 2 cut(s) 215, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.