Rorug04G0221900

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005575
Physical Location & Seq
Forward (+)
38126900 .. 38128172
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0221900.1

Sequence Viewer

Length: 438 bp
ATGGCGTCCAGTACGTCCGATCCCCTACGACGTCGTTTCTGCGTTCTTCGCAGGAGCGGAAGCTTTGAATCGTCTTTGTGGAGTTGGAGATATTTGGTAGCGGCGGCGCGTATTTCCAGGAGTTGCTTCGGAGTTCCTCAGATCTCTTCCCCTAGATCCCTCCCAGGGAGGAAGACTGGCTTTCTTGTGGTTAGATCTGATGCCGGAGTTAACCCGAATGCTAGAGCTCACCAGACCGATCAGTTGAGCACCAATGTTGTTGCGACAAAAGCTGCTGCTCCTGTGTATGTCTATGTGAAGTTTTTAGCTTTTTACTGTTATGCTTATGTTGTTTTTGCTAAATTTGATTCTGATATAGTTTTGAGATCGATCACAGGTTTTGGAGATGGTAACTGTGTGGTAGTCAATGTTAAGAATCTATGGTTGTATGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

145

Amino Acids

16.15

Weight (kDa)

9.9

Isoelectric Point (pI)

63.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 57
AccII CGCG 1 cut(s) 109
AciI CCGC 3 cut(s) 57, 101, 104
AclWI GGATC 2 cut(s) 14, 150
AcsI RAATTY 1 cut(s) 341
AcyI GRCGYC 2 cut(s) 5, 31
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 165
AgsI TTSAA 1 cut(s) 68
AjnI CCWGG 2 cut(s) 116, 163
AluBI AGCT 4 cut(s) 63, 227, 272, 308
AluI AGCT 4 cut(s) 63, 227, 272, 308
Alw21I GWGCWC 2 cut(s) 229, 251
AlwI GGATC 2 cut(s) 14, 150
ApeKI GCWGC 2 cut(s) 272, 275
ApoI RAATTY 1 cut(s) 341
AspLEI GCGC 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 221
BanII GRGCYC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 229, 251
BbvI GCAGC 2 cut(s) 259, 262
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 2 cut(s) 118, 165
BfaI CTAG 3 cut(s) 153, 222, 436
BglII AGATCT 2 cut(s) 141, 194
BisI GCNGC 4 cut(s) 102, 105, 273, 276
BlsI GCNGC 4 cut(s) 103, 106, 274, 277
Bme1390I CCNGG 2 cut(s) 118, 165
BmrFI CCNGG 2 cut(s) 118, 165
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 368
BsaHI GRCGYC 2 cut(s) 5, 31
BsaJI CCNNGG 2 cut(s) 163, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse1I ACTGG 2 cut(s) 9, 181
BseBI CCWGG 2 cut(s) 118, 165
BseCI ATCGAT 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 163, 164
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 2 cut(s) 9, 181
BseXI GCAGC 2 cut(s) 259, 262
Bsh1236I CGCG 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 368
BsiHKAI GWGCWC 2 cut(s) 229, 251
BsiSI CCGG 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 165
BsmI GAATGC 1 cut(s) 223
Bsp1286I GDGCHC 2 cut(s) 229, 251
Bsp143I GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
BspACI CCGC 3 cut(s) 57, 101, 104
BspCNI CTCAG 1 cut(s) 151
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 1 cut(s) 109
BspPI GGATC 2 cut(s) 14, 150
BsrBI CCGCTC 1 cut(s) 57
BsrI ACTGG 2 cut(s) 9, 181
BssECI CCNNGG 2 cut(s) 163, 164
BssMI GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
BssNI GRCGYC 2 cut(s) 5, 31
Bst2UI CCWGG 2 cut(s) 118, 165
Bst4CI ACNGT 2 cut(s) 317, 395
Bst6I CTCTTC 1 cut(s) 151
BstACI GRCGYC 2 cut(s) 5, 31
BstDEI CTNAG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 109
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 7 cut(s) 22, 144, 158, 197, 241, 368, 372
BstMBI GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
BstMWI GCNNNNNNNGC 2 cut(s) 48, 269
BstNI CCWGG 2 cut(s) 118, 165
BstSCI CCNGG 2 cut(s) 116, 163
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 259, 262
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 3 cut(s) 141, 155, 194
BstYI RGATCY 3 cut(s) 141, 155, 194
Bsu15I ATCGAT 1 cut(s) 368
BsuTUI ATCGAT 1 cut(s) 368
CfoI GCGC 1 cut(s) 109
ClaI ATCGAT 1 cut(s) 368
Csp6I GTAC 1 cut(s) 12
CviJI RGCY 5 cut(s) 63, 180, 227, 272, 308
CviKI_1 RGCY 5 cut(s) 63, 180, 227, 272, 308
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 1 cut(s) 138
DpnI GATC 7 cut(s) 21, 143, 157, 196, 240, 367, 371
DpnII GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 116, 163
EcoT38I GRGCYC 1 cut(s) 229
FaiI YATR 7 cut(s) 288, 294, 321, 327, 356, 421, 429
FalI AAGNNNNNCTT 2 cut(s) 164, 196
Fnu4HI GCNGC 4 cut(s) 102, 105, 273, 276
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 4 cut(s) 102, 105, 273, 276
FspBI CTAG 3 cut(s) 153, 222, 436
GlaI GCGC 1 cut(s) 108
GluI GCNGC 4 cut(s) 102, 105, 273, 276
HapII CCGG 1 cut(s) 204
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 2 cut(s) 5, 31
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 3 cut(s) 68, 347, 415
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 5 cut(s) 19, 131, 141, 199, 352
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 2 cut(s) 33, 36
HpyCH4III ACNGT 2 cut(s) 317, 395
HpyCH4IV ACGT 2 cut(s) 14, 31
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 269
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 2 cut(s) 14, 31
Hsp92I GRCGYC 2 cut(s) 5, 31
HspAI GCGC 1 cut(s) 107
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
LmnI GCTCC 2 cut(s) 54, 283
Lsp1109I GCAGC 2 cut(s) 259, 262
LweI GCATC 1 cut(s) 190
MaeI CTAG 3 cut(s) 153, 222, 436
MaeII ACGT 2 cut(s) 14, 31
MaeIII GTNAC 1 cut(s) 389
MalI GATC 7 cut(s) 21, 143, 157, 196, 240, 367, 371
MbiI CCGCTC 1 cut(s) 57
MboI GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
MboII GAAGA 3 cut(s) 38, 138, 184
MflI RGATCY 3 cut(s) 141, 155, 194
MhlI GDGCHC 2 cut(s) 229, 251
MluCI AATT 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 3 cut(s) 147, 162, 170
MseI TTAA 2 cut(s) 210, 411
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 2 cut(s) 118, 165
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 2 cut(s) 118, 165
MvnI CGCG 1 cut(s) 109
MwoI GCNNNNNNNGC 2 cut(s) 48, 269
NdeII GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
PasI CCCWGGG 1 cut(s) 164
PctI GAATGC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 68, 347, 415
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 4 cut(s) 103, 106, 274, 277
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 116, 163
PspGI CCWGG 2 cut(s) 116, 163
PsuI RGATCY 3 cut(s) 141, 155, 194
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 2 cut(s) 210, 411
SatI GCNGC 4 cut(s) 102, 105, 273, 276
Sau3AI GATC 7 cut(s) 19, 141, 155, 194, 238, 365, 369
ScrFI CCNGG 2 cut(s) 118, 165
SduI GDGCHC 2 cut(s) 229, 251
SetI ASST 7 cut(s) 17, 34, 65, 229, 274, 310, 379
SfaNI GCATC 1 cut(s) 190
Sse9I AATT 1 cut(s) 341
SsiI CCGC 3 cut(s) 57, 101, 104
SspMI CTAG 3 cut(s) 153, 222, 436
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 116, 163
TaaI ACNGT 2 cut(s) 317, 395
TaiI ACGT 2 cut(s) 17, 34
TaqI TCGA 1 cut(s) 368
TaqII GACCGA 1 cut(s) 251
TasI AATT 1 cut(s) 341
TauI GCSGC 2 cut(s) 104, 107
TfiI GAWTC 3 cut(s) 68, 347, 415
Tru1I TTAA 2 cut(s) 210, 411
Tru9I TTAA 2 cut(s) 210, 411
TseI GCWGC 2 cut(s) 272, 275
XapI RAATTY 1 cut(s) 341
XspI CTAG 3 cut(s) 153, 222, 436
ZraI GACGTC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.