Rroxscaffold_5G00369760

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005575
Physical Location & Seq
Reverse (-)
51562664 .. 51566883
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369760.1

Sequence Viewer

Length: 1224 bp
ATGAAGCTGTTCTGGGTTTTTGGGGGGTTTATGGGGTTGATGTTTTGCAGACAAACAGATGGTTTTGTAGATTCGAATGTAACTCAAGATGAAGACTTGCATTATAGTGAGATTAATGTGTTTGGTTATACTAATGGGTTTTCCAGCACACCCTTGATGGTGGGTCTTACTCTCATTGGTGGAGCAGCTGCTAAAGGAGCAGTCTGCCTAGATGGAACATTACCTGCATACCATATCCACCGGGGATACGGATCAGGAGCAAACAGTTGGCTCATTGACTTGGAGGGAGGAGGATGGTGTGATACAATCAGAAATTGTGTCTATCGCAAGAAAACTCGTCGCGGTTCATCAGCATATATGGAGAAGCATATACCATTCACAGGGATATTGAGCAACAAAGCTAGTGAAAACCCTGACTTTTTCAACTGGAACAGAGTCAAGGTTCGATATTGTGATGGTGCATCATTTGCTGGAGACAGTGAAAATAAGGCAGCCCAATTGCATTTTCGAGGTCAGCGTATATGGAAAGCTGCTATGGAAGATCTGATGTCCAAGGGAATGCACTATGCTAAACAGGCTCTTCTTTCTGGATGTTCTGCTGGTGCTGTTGCAACTGTCCTCCACTGTGATGAATTCCGTGAAATGTTTCCTGGAACTACTAGAGTCAAGTGCCTCAGTGATGCTGGATTATTTCTTGATGTATTTGATGTATCTGGTAGCCGCACTTTGAGAAATATGTTTAGACGGGTGGTTAAACTACAGGGAGTGAAGAAGAACCTTCCACGGAGCTGTACTAATCGCCTCAGCCCGAATTTGTGCTTTTTCCCTCAGCACATAATTGCAAGTGTCAAGACCCCCCTCTTTCTAGTCAATGCAGCTTATGATACATGGCAGATCCAGGCAAGTTTAGCTCCACCAATAGCAGATCCTAATGGCAGTTGGAATGAATGCAAAAAGAATCATGCACGTTGCACTCTGCGGCAGATTAACTTTTTCAAAGGCTTCCGGAATCAAATGCTCAAAGCTGTAAGCGGCTTCTCAAGGGCTGGTAAAAATGGTTTGTTCATAAACTCATGCTTTGCACATTGCCAAACCGAGAGACAGGACACATGGTTTGCTCAAGATTCTCCTCATATCGGAAATAAGGGGATAGCAAAATCAGTGGGGAACTGGTACTTTGATAGACTTAATATCAAGGCTATAGATTTGTCCTTACCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

407

Amino Acids

45.38

Weight (kDa)

9.3

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 49 - 406 3e-165 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 1005
AciI CCGC 4 cut(s) 342, 721, 979, 1032
AclWI GGATC 3 cut(s) 259, 889, 920
AcsI RAATTY 2 cut(s) 632, 811
AfaI GTAC 2 cut(s) 793, 1175
AfiI CCNNNNNNNGG 2 cut(s) 380, 1136
AgsI TTSAA 2 cut(s) 424, 997
AjnI CCWGG 2 cut(s) 649, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 8 cut(s) 7, 188, 401, 530, 789, 878, 911, 1025
AluI AGCT 8 cut(s) 7, 188, 401, 530, 789, 878, 911, 1025
Alw26I GTCTC 2 cut(s) 468, 1093
AlwI GGATC 3 cut(s) 259, 889, 920
Aor13HI TCCGGA 1 cut(s) 1005
ApeKI GCWGC 5 cut(s) 185, 188, 491, 530, 875
ApoI RAATTY 2 cut(s) 632, 811
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 2 cut(s) 8, 645
AsuC2I CCSGG 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 99
BbvCI CCTCAGC 2 cut(s) 803, 828
BbvI GCAGC 5 cut(s) 175, 197, 503, 517, 887
BccI CCATC 5 cut(s) 53, 151, 206, 288, 449
BciT130I CCWGG 2 cut(s) 651, 899
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 2 cut(s) 468, 1093
BfaI CTAG 4 cut(s) 209, 402, 660, 866
BfmI CTRYAG 2 cut(s) 758, 1200
BfuAI ACCTGC 1 cut(s) 232
BfuI GTATCC 1 cut(s) 239
BglII AGATCT 1 cut(s) 541
BisI GCNGC 8 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033
BlsI GCNGC 8 cut(s) 187, 190, 493, 532, 722, 877, 981, 1034
Bme1390I CCNGG 3 cut(s) 242, 651, 899
BmrFI CCNGG 3 cut(s) 242, 651, 899
BmsI GCATC 2 cut(s) 470, 670
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 2 cut(s) 803, 828
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 3 cut(s) 69, 1024, 1104
BpuMI CCSGG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 241, 552, 782
BsaWI WCCGGW 1 cut(s) 1005
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 1136
Bse1I ACTGG 2 cut(s) 431, 1175
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 1 cut(s) 250
BseAI TCCGGA 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 651, 899
BseDI CCNNGG 3 cut(s) 241, 552, 782
BseGI GGATG 2 cut(s) 299, 596
BseJI GATNNNNATC 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 380, 1136
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 3 cut(s) 688, 817, 842
BseNI ACTGG 2 cut(s) 431, 1175
BseRI GAGGAG 2 cut(s) 303, 1119
BseXI GCAGC 5 cut(s) 175, 197, 503, 517, 887
Bsh1236I CGCG 1 cut(s) 342
BsiSI CCGG 2 cut(s) 241, 1006
BslI CCNNNNNNNGG 2 cut(s) 380, 1136
BsmAI GTCTC 2 cut(s) 468, 1093
BsmI GAATGC 2 cut(s) 564, 953
Bsp119I TTCGAA 1 cut(s) 74
Bsp13I TCCGGA 1 cut(s) 1005
Bsp143I GATC 4 cut(s) 251, 541, 894, 925
BspACI CCGC 4 cut(s) 342, 721, 979, 1032
BspCNI CTCAG 3 cut(s) 687, 816, 841
BspEI TCCGGA 1 cut(s) 1005
BspFNI CGCG 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 3 cut(s) 259, 889, 920
BspQI GCTCTTC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 1084
BsrI ACTGG 2 cut(s) 431, 1175
BssECI CCNNGG 3 cut(s) 241, 552, 782
BssMI GATC 4 cut(s) 251, 541, 894, 925
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 651, 899
Bst4CI ACNGT 4 cut(s) 266, 479, 616, 626
Bst6I CTCTTC 1 cut(s) 585
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 674, 803, 828
BstDSI CCRYGG 1 cut(s) 782
BstF5I GGATG 2 cut(s) 299, 596
BstFNI CGCG 1 cut(s) 342
BstKTI GATC 4 cut(s) 254, 544, 897, 928
BstMAI GTCTC 2 cut(s) 468, 1093
BstMBI GATC 4 cut(s) 251, 541, 894, 925
BstMWI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
BstNI CCWGG 2 cut(s) 651, 899
BstSCI CCNGG 3 cut(s) 240, 649, 897
BstSFI CTRYAG 2 cut(s) 758, 1200
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 5 cut(s) 175, 197, 503, 517, 887
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 3 cut(s) 541, 894, 925
BstYI RGATCY 3 cut(s) 541, 894, 925
BsuI GTATCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 782
BtsCI GGATG 2 cut(s) 299, 596
BtsIMutI CAGTG 4 cut(s) 484, 622, 682, 1167
BveI ACCTGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 792, 1174
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviAII CATG 4 cut(s) 888, 962, 1074, 1110
CviQI GTAC 2 cut(s) 792, 1174
DdeI CTNAG 3 cut(s) 674, 803, 828
DpnI GATC 4 cut(s) 253, 543, 896, 927
DpnII GATC 4 cut(s) 251, 541, 894, 925
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 649, 897
EcoT14I CCWWGG 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 4 cut(s) 891, 965, 1077, 1113
FatI CATG 4 cut(s) 887, 961, 1073, 1109
Fnu4HI GCNGC 8 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033
FokI GGATG 2 cut(s) 306, 603
Fsp4HI GCNGC 8 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033
FspBI CTAG 4 cut(s) 209, 402, 660, 866
GluI GCNGC 8 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033
GsuI CTGGAG 1 cut(s) 492
HapII CCGG 2 cut(s) 241, 1006
Hin1II CATG 4 cut(s) 891, 965, 1077, 1113
HinfI GANTC 6 cut(s) 71, 435, 663, 958, 1009, 1124
HpaII CCGG 2 cut(s) 241, 1006
Hpy188I TCNGA 3 cut(s) 311, 546, 1139
Hpy188III TCNNGA 7 cut(s) 86, 255, 588, 695, 850, 1006, 1121
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 1 cut(s) 788
HpyCH4III ACNGT 4 cut(s) 266, 479, 616, 626
HpyCH4IV ACGT 1 cut(s) 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
HpyF3I CTNAG 3 cut(s) 674, 803, 828
HpySE526I ACGT 1 cut(s) 967
Hsp92II CATG 4 cut(s) 891, 965, 1077, 1113
Kpn2I TCCGGA 1 cut(s) 1005
Kzo9I GATC 4 cut(s) 251, 541, 894, 925
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 5 cut(s) 182, 197, 257, 786, 916
Lsp1109I GCAGC 5 cut(s) 175, 197, 503, 517, 887
LweI GCATC 2 cut(s) 470, 670
MaeI CTAG 4 cut(s) 209, 402, 660, 866
MaeII ACGT 1 cut(s) 967
MaeIII GTNAC 1 cut(s) 79
MalI GATC 4 cut(s) 253, 543, 896, 927
MboI GATC 4 cut(s) 251, 541, 894, 925
MboII GAAGA 5 cut(s) 104, 551, 572, 781, 784
MfeI CAATTG 1 cut(s) 497
MflI RGATCY 3 cut(s) 541, 894, 925
MluCI AATT 5 cut(s) 313, 497, 632, 811, 837
MlyI GAGTC 2 cut(s) 444, 672
MmeI TCCRAC 1 cut(s) 920
MroI TCCGGA 1 cut(s) 1005
MroXI GAANNNNTTC 2 cut(s) 8, 645
MseI TTAA 4 cut(s) 114, 753, 987, 1188
MslI CAYNNNNRTG 2 cut(s) 105, 627
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 2 cut(s) 241, 1006
MspR9I CCNGG 3 cut(s) 242, 651, 899
MunI CAATTG 1 cut(s) 497
Mva1269I GAATGC 2 cut(s) 564, 953
MvaI CCWGG 2 cut(s) 651, 899
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 251, 541, 894, 925
NlaIII CATG 4 cut(s) 891, 965, 1077, 1113
NspV TTCGAA 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 585
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 2 cut(s) 564, 953
PdmI GAANNNNTTC 2 cut(s) 8, 645
PfeI GAWTC 4 cut(s) 71, 958, 1009, 1124
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 8 cut(s) 187, 190, 493, 532, 722, 877, 981, 1034
PleI GAGTC 2 cut(s) 443, 671
PpsI GAGTC 2 cut(s) 443, 671
PshBI ATTAAT 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 649, 897
PspGI CCWGG 2 cut(s) 649, 897
PsuI RGATCY 3 cut(s) 541, 894, 925
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 793, 1175
RsaNI GTAC 2 cut(s) 792, 1174
RseI CAYNNNNRTG 2 cut(s) 105, 627
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 4 cut(s) 114, 753, 987, 1188
SatI GCNGC 8 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033
Sau3AI GATC 4 cut(s) 251, 541, 894, 925
SchI GAGTC 2 cut(s) 444, 672
ScrFI CCNGG 3 cut(s) 242, 651, 899
SfaNI GCATC 2 cut(s) 470, 670
SfcI CTRYAG 2 cut(s) 758, 1200
SfuI TTCGAA 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 105, 627
SmlI CTYRAG 3 cut(s) 84, 1039, 1119
SmoI CTYRAG 3 cut(s) 84, 1039, 1119
Sse9I AATT 5 cut(s) 313, 497, 632, 811, 837
SsiI CCGC 4 cut(s) 342, 721, 979, 1032
SspMI CTAG 4 cut(s) 209, 402, 660, 866
StyD4I CCNGG 3 cut(s) 240, 649, 897
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 266, 479, 616, 626
TaiI ACGT 1 cut(s) 970
TaqI TCGA 3 cut(s) 74, 445, 508
TasI AATT 5 cut(s) 313, 497, 632, 811, 837
TatI WGTACW 1 cut(s) 791
TauI GCSGC 3 cut(s) 723, 982, 1035
TfiI GAWTC 4 cut(s) 71, 958, 1009, 1124
Tru1I TTAA 4 cut(s) 114, 753, 987, 1188
Tru9I TTAA 4 cut(s) 114, 753, 987, 1188
TscAI CASTG 4 cut(s) 484, 629, 682, 1167
TseI GCWGC 5 cut(s) 185, 188, 491, 530, 875
TspDTI ATGAA 6 cut(s) 17, 105, 336, 645, 960, 1054
TspGWI ACGGA 3 cut(s) 264, 626, 799
TspRI CASTG 4 cut(s) 484, 629, 682, 1167
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 2 cut(s) 632, 811
XmnI GAANNNNTTC 2 cut(s) 8, 645
XspI CTAG 4 cut(s) 209, 402, 660, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.