Rh4AG279000

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005575
Physical Location & Seq
Reverse (-)
59506851 .. 59510904
4054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG279000.1

Sequence Viewer

Length: 1254 bp
ATGAAGCTGTTCTGGGTTTTTGGGGGGTTTATGGGGTTGATGTTTTGCAGACAAACAGATGGTTTTGTAGATTGGAATGTAACTCAAGATGAAGACTTGCATTATAGTGAGATTAATGTGTTTGGTTATACTAATGGGTTTTCCAGCACACCCTTGATGGTGGGTCTTACTCTCATTGGTGGAGCTGCTGCTAAAGGAGCAGTCTGCCTAGATGGAACATTACCTGCATACCATATCCACCGGGGATACGGATCAGGAGCAAACAGTTGGCTCATTGACTTGGAGGGAGGAGGATGGTGTGATACAATCAGAAATTGTGTCTATCGCAAGAAAACTCGTCGTGGTTCATCAGCATATATGGAGAAGCATATACCATTCACAGGGATATTGAGCAACAAAGCTAGTGAAAACCCTGACTTTTTCAACTGGAACAGAGTCAAGGTTCGATATTGTGATGGTGCATCTTTTGCTGGAGACAGTGAAAATAAGGCAGCCCAATTGCATTTTCGAGGTCAGCGTATATGGAAAGCTGCTATGGAAGATCTGATGTCCAAGGGAATGCGCTATGCCAAACAGGCCCTTCTTTCTGGATGTTCTGCTGGTGCTGTTGCAACTGTCCTCCACTGTGATGAATTCCGTGAAATGTTTCCTGGAACTACTAGAGTCAAGTGCCTCAGTGATGCTGGATTATTTCTTGATGCATTTGATGTATCTGGTAGCCGCACTTTGAGAAATATGTTTAGACGGGTGGTTAACCTACAGGGAGCAAAGAAGAACCTTCCACGGAGTTGTACTAATCGCCTCAACCCGAATTTGTGCTTTTTCCCTCAACACATAATTGCAAGTGTCAAGACCCCCCTCTTTCTAGTCAATGCAGCTTATGATACATGGCAGATCCAGGCAAGTTTAGCTCCACCAATAGCAGATCCTAATGGCAGTTGGAATGAATGCAAAAAGAATCATGCACGTTGCACTCTGCGGCAGATTAACTTTTTTCAAAGCTTCCGGAATCAAATGCTCAAAGCTGTAAGCGGCTTCTCAAGGGCTGGTAAAAATGGTTTGTTCATAAACTCATGCTTTGCACATTGCCAAACCGAGAGACAGGACACATGGTTTGCTCAAGATTCTCCTCATATAGGAAATAAGGGGATAGCAAAATCTGTGGGGAACTGGTACTTTGATAGACTAAATATCAAGGCTATAGATTGTCCTTACCCTTGTGACCAGAGCTGCCATAATATCGTGTTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

417

Amino Acids

46.7

Weight (kDa)

9.14

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 49 - 412 6.4e-172 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccIII TCCGGA 1 cut(s) 1005
AciI CCGC 3 cut(s) 721, 979, 1032
AclWI GGATC 3 cut(s) 259, 889, 920
AcsI RAATTY 2 cut(s) 632, 811
AfaI GTAC 2 cut(s) 793, 1175
AfiI CCNNNNNNNGG 2 cut(s) 380, 1136
AgsI TTSAA 2 cut(s) 424, 998
AjnI CCWGG 2 cut(s) 649, 897
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 9 cut(s) 7, 185, 401, 530, 878, 911, 1002, 1025, 1230
AluI AGCT 9 cut(s) 7, 185, 401, 530, 878, 911, 1002, 1025, 1230
Alw26I GTCTC 2 cut(s) 468, 1093
AlwI GGATC 3 cut(s) 259, 889, 920
Aor13HI TCCGGA 1 cut(s) 1005
AoxI GGCC 1 cut(s) 576
ApeKI GCWGC 6 cut(s) 185, 188, 491, 530, 875, 1230
ApoI RAATTY 2 cut(s) 632, 811
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 2 cut(s) 8, 645
AspLEI GCGC 1 cut(s) 564
AspS9I GGNCC 1 cut(s) 577
AsuC2I CCSGG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 6 cut(s) 172, 175, 503, 517, 887, 1217
BccI CCATC 5 cut(s) 53, 151, 206, 288, 449
BciT130I CCWGG 2 cut(s) 651, 899
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 2 cut(s) 468, 1093
BfaI CTAG 4 cut(s) 209, 402, 660, 866
BfmI CTRYAG 2 cut(s) 758, 1200
BfuAI ACCTGC 1 cut(s) 232
BfuI GTATCC 1 cut(s) 239
BglI GCCNNNNNGGC 1 cut(s) 575
BglII AGATCT 1 cut(s) 541
BisI GCNGC 9 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033, 1231
BlsI GCNGC 9 cut(s) 187, 190, 493, 532, 722, 877, 981, 1034, 1232
Bme1390I CCNGG 3 cut(s) 242, 651, 899
BmgT120I GGNCC 1 cut(s) 577
BmrFI CCNGG 3 cut(s) 242, 651, 899
BmsI GCATC 3 cut(s) 470, 670, 688
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 3 cut(s) 69, 1024, 1104
BpuMI CCSGG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 241, 552, 782
BsaWI WCCGGW 1 cut(s) 1005
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 1136
Bse1I ACTGG 2 cut(s) 431, 1175
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 1 cut(s) 250
BseAI TCCGGA 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 651, 899
BseDI CCNNGG 3 cut(s) 241, 552, 782
BseGI GGATG 2 cut(s) 299, 596
BseJI GATNNNNATC 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 380, 1136
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 1 cut(s) 688
BseNI ACTGG 2 cut(s) 431, 1175
BseRI GAGGAG 2 cut(s) 303, 1119
BseXI GCAGC 6 cut(s) 172, 175, 503, 517, 887, 1217
BshFI GGCC 1 cut(s) 578
BsiSI CCGG 2 cut(s) 241, 1006
BslI CCNNNNNNNGG 2 cut(s) 380, 1136
BsmAI GTCTC 2 cut(s) 468, 1093
BsmI GAATGC 2 cut(s) 564, 953
BsnI GGCC 1 cut(s) 578
Bsp13I TCCGGA 1 cut(s) 1005
Bsp143I GATC 4 cut(s) 251, 541, 894, 925
BspACI CCGC 3 cut(s) 721, 979, 1032
BspANI GGCC 1 cut(s) 578
BspCNI CTCAG 1 cut(s) 687
BspEI TCCGGA 1 cut(s) 1005
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 3 cut(s) 259, 889, 920
BsrDI GCAATG 1 cut(s) 1084
BsrI ACTGG 2 cut(s) 431, 1175
BssECI CCNNGG 3 cut(s) 241, 552, 782
BssMI GATC 4 cut(s) 251, 541, 894, 925
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 651, 899
Bst4CI ACNGT 4 cut(s) 266, 479, 616, 626
BstAPI GCANNNNNTGC 1 cut(s) 467
BstDEI CTNAG 1 cut(s) 674
BstDSI CCRYGG 1 cut(s) 782
BstENI CCTNNNNNAGG 1 cut(s) 1134
BstF5I GGATG 2 cut(s) 299, 596
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 4 cut(s) 254, 544, 897, 928
BstMAI GTCTC 2 cut(s) 468, 1093
BstMBI GATC 4 cut(s) 251, 541, 894, 925
BstMWI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
BstNI CCWGG 2 cut(s) 651, 899
BstSCI CCNGG 3 cut(s) 240, 649, 897
BstSFI CTRYAG 2 cut(s) 758, 1200
BstV1I GCAGC 6 cut(s) 172, 175, 503, 517, 887, 1217
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 3 cut(s) 541, 894, 925
BstYI RGATCY 3 cut(s) 541, 894, 925
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 578
BtgI CCRYGG 1 cut(s) 782
BtsCI GGATG 2 cut(s) 299, 596
BtsIMutI CAGTG 3 cut(s) 484, 622, 682
BveI ACCTGC 1 cut(s) 232
CfoI GCGC 1 cut(s) 564
Cfr13I GGNCC 1 cut(s) 577
Csp6I GTAC 2 cut(s) 792, 1174
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviAII CATG 4 cut(s) 888, 962, 1074, 1110
CviQI GTAC 2 cut(s) 792, 1174
DdeI CTNAG 1 cut(s) 674
DpnI GATC 4 cut(s) 253, 543, 896, 927
DpnII GATC 4 cut(s) 251, 541, 894, 925
Eco130I CCWWGG 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 649, 897
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 4 cut(s) 891, 965, 1077, 1113
FatI CATG 4 cut(s) 887, 961, 1073, 1109
Fnu4HI GCNGC 9 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033, 1231
FokI GGATG 2 cut(s) 306, 603
Fsp4HI GCNGC 9 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033, 1231
FspBI CTAG 4 cut(s) 209, 402, 660, 866
GlaI GCGC 1 cut(s) 563
GluI GCNGC 9 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033, 1231
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 1 cut(s) 578
HapII CCGG 2 cut(s) 241, 1006
HhaI GCGC 1 cut(s) 564
Hin1II CATG 4 cut(s) 891, 965, 1077, 1113
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HindIII AAGCTT 1 cut(s) 1000
HinfI GANTC 5 cut(s) 435, 663, 958, 1009, 1124
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 2 cut(s) 241, 1006
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 2 cut(s) 311, 546
Hpy188III TCNNGA 7 cut(s) 86, 255, 588, 695, 850, 1006, 1121
Hpy8I GTNNAC 1 cut(s) 754
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 2 cut(s) 590, 788
HpyCH4III ACNGT 4 cut(s) 266, 479, 616, 626
HpyCH4IV ACGT 1 cut(s) 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
HpyF3I CTNAG 1 cut(s) 674
HpySE526I ACGT 1 cut(s) 967
Hsp92II CATG 4 cut(s) 891, 965, 1077, 1113
HspAI GCGC 1 cut(s) 562
Kpn2I TCCGGA 1 cut(s) 1005
KspAI GTTAAC 1 cut(s) 754
Kzo9I GATC 4 cut(s) 251, 541, 894, 925
LmnI GCTCC 5 cut(s) 182, 197, 257, 764, 916
Lsp1109I GCAGC 6 cut(s) 172, 175, 503, 517, 887, 1217
LweI GCATC 3 cut(s) 470, 670, 688
MaeI CTAG 4 cut(s) 209, 402, 660, 866
MaeII ACGT 1 cut(s) 967
MaeIII GTNAC 2 cut(s) 79, 1220
MalI GATC 4 cut(s) 253, 543, 896, 927
MboI GATC 4 cut(s) 251, 541, 894, 925
MboII GAAGA 3 cut(s) 104, 551, 784
MfeI CAATTG 1 cut(s) 497
MflI RGATCY 3 cut(s) 541, 894, 925
MluCI AATT 5 cut(s) 313, 497, 632, 811, 837
MlyI GAGTC 2 cut(s) 444, 672
MmeI TCCRAC 1 cut(s) 920
Mph1103I ATGCAT 1 cut(s) 703
MroI TCCGGA 1 cut(s) 1005
MroXI GAANNNNTTC 2 cut(s) 8, 645
MseI TTAA 4 cut(s) 114, 753, 987, 1248
MslI CAYNNNNRTG 2 cut(s) 105, 627
MspI CCGG 2 cut(s) 241, 1006
MspR9I CCNGG 3 cut(s) 242, 651, 899
MunI CAATTG 1 cut(s) 497
Mva1269I GAATGC 2 cut(s) 564, 953
MvaI CCWGG 2 cut(s) 651, 899
MwoI GCNNNNNNNGC 4 cut(s) 197, 467, 575, 908
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 251, 541, 894, 925
NlaIII CATG 4 cut(s) 891, 965, 1077, 1113
NmuCI GTSAC 1 cut(s) 1220
NsiI ATGCAT 1 cut(s) 703
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 2 cut(s) 564, 953
PdmI GAANNNNTTC 2 cut(s) 8, 645
PfeI GAWTC 3 cut(s) 958, 1009, 1124
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 9 cut(s) 187, 190, 493, 532, 722, 877, 981, 1034, 1232
PleI GAGTC 2 cut(s) 443, 671
PpsI GAGTC 2 cut(s) 443, 671
PshBI ATTAAT 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 649, 897
PspGI CCWGG 2 cut(s) 649, 897
PspPI GGNCC 1 cut(s) 577
PsuI RGATCY 3 cut(s) 541, 894, 925
RsaI GTAC 2 cut(s) 793, 1175
RsaNI GTAC 2 cut(s) 792, 1174
RseI CAYNNNNRTG 2 cut(s) 105, 627
SaqAI TTAA 4 cut(s) 114, 753, 987, 1248
SatI GCNGC 9 cut(s) 186, 189, 492, 531, 721, 876, 980, 1033, 1231
Sau3AI GATC 4 cut(s) 251, 541, 894, 925
Sau96I GGNCC 1 cut(s) 577
SchI GAGTC 2 cut(s) 444, 672
ScrFI CCNGG 3 cut(s) 242, 651, 899
SfaNI GCATC 3 cut(s) 470, 670, 688
SfcI CTRYAG 2 cut(s) 758, 1200
SmiMI CAYNNNNRTG 2 cut(s) 105, 627
SmlI CTYRAG 3 cut(s) 84, 1039, 1119
SmoI CTYRAG 3 cut(s) 84, 1039, 1119
Sse9I AATT 5 cut(s) 313, 497, 632, 811, 837
SsiI CCGC 3 cut(s) 721, 979, 1032
SspMI CTAG 4 cut(s) 209, 402, 660, 866
StyD4I CCNGG 3 cut(s) 240, 649, 897
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 266, 479, 616, 626
TaiI ACGT 1 cut(s) 970
TaqI TCGA 2 cut(s) 445, 508
TasI AATT 5 cut(s) 313, 497, 632, 811, 837
TatI WGTACW 1 cut(s) 791
TauI GCSGC 3 cut(s) 723, 982, 1035
TfiI GAWTC 3 cut(s) 958, 1009, 1124
Tru1I TTAA 4 cut(s) 114, 753, 987, 1248
Tru9I TTAA 4 cut(s) 114, 753, 987, 1248
TscAI CASTG 3 cut(s) 484, 629, 682
TseFI GTSAC 1 cut(s) 1220
TseI GCWGC 6 cut(s) 185, 188, 491, 530, 875, 1230
Tsp45I GTSAC 1 cut(s) 1220
TspDTI ATGAA 6 cut(s) 17, 105, 336, 645, 960, 1054
TspGWI ACGGA 3 cut(s) 264, 626, 799
TspRI CASTG 3 cut(s) 484, 629, 682
VspI ATTAAT 1 cut(s) 114
XagI CCTNNNNNAGG 1 cut(s) 1134
XapI RAATTY 2 cut(s) 632, 811
XmnI GAANNNNTTC 2 cut(s) 8, 645
XspI CTAG 4 cut(s) 209, 402, 660, 866
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.