AT1G57590

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005575
Physical Location & Seq
Reverse (-)
21327360 .. 21329944
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G57590.1

Sequence Viewer

Length: 1335 bp
ATGCTCTTAAGTCACTCTCGTTCTCCATATAAACCAAGGTATGTGATCAAGTCTAAAACATCCATGATCAAGAAGTGTAAAATGAAGAAGCTTCTATGGAGTTGGATCATTCTCTTCAACATCCATGTAAATGGAATGATGATGGAGTTTGATGAAATGGAGTGGTTCACAGTTTTCAATGGAACAAAAGTGTTTCAGACTCAAAATGATGTTTTTTCTGAAGCTAAGTTTCCTATGGTTGGACTCACTCTGATTCAATCTGCTGCTGCTAAAGGAGCTGTGTGTTTGGATGGAAGTCTTCCCGGATACCATTTGCATCGCGGGTTTGGTTCCGGAGCGAACAATTGGCTCGTTCAATTAGAGGGAGGTGGATGGTGTGATACCATTAGAAATTGTGTTTACCGTAAAACCACTCGTCGTGGATCTTCTTCATATATGGAGAAAGAGATACCATTTACCGGCATTCTAAGTGATAAAGCCGCGGATAATCCAGACTTTTATAACTGGAATAGAGTAAAAGTTCGGTATTGTGACGGTGGATCGTTTAGCGGGGATAGCGAAAATAAGGCTGCACAACTTCAGTTTAGAGGAAAGAGAATTTGGTTAGCTGCTATGGAAGATTTGATGGCAAAAGGTATGCGTCAAGCTAAGCAGGCTCTGCTCTCTGGATGTTCTGCAGGAGGTCTTGCGGTGATTTTACGATGTGATGATTTTGGGAAATTGTTTCCTCCTTCCACCAGAGTGAAATGTTTGAGTGATGCTGGTTTCTTCCTTGATGCCATCGATGTGTCTGGAGGTCGATCTCTTAGACGATTATACGCCGGTGTGGTCAGATTACAGAATCTGCAAACCAACCTTCCTCAGTATTGTGTAAACCGTCTTAATCCCACTTCGTGTTTTTTCCCACAAAACCTAATCAACCAAGTCAAGACTCCACTTTTTATTCTTAATGCTGCATATGATTCTTGGCAGATTCAAGAGAGTTTAGCTCCAAAATCTGCAGATCCAAGTGGAAGTTGGAATGATTGTAGACTTAACTACGCCAAGTGTAGTGCATCTCAGATCCAATTCTTGCAAGGTTTTAGGACGCGTATGGTGAATTTGGTAAAAGGTTTCGCGATGCCAAGTAAGAACGGTGTGTTCCTCAATTCGTGCTTTGCTCATTGCCAAACAGAGAGGCATGACACTTGGTTCGCCCAAAACTCTCCAGCTATTAAGAACAAGGGTATTGCAGTAGCTGTGGGAGATTGGTATTTCGAAAGAGGAGGAGCAAAGCTCATAGACTGTGCTTATCCTTGTGACAAGACTTGTCACAATTTGGTCTTTAGAAGATAA

Protein Analysis

444

Amino Acids

49.91

Weight (kDa)

9.41

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 78 - 438 5.5e-175 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccI GTMKAC 1 cut(s) 1030
AccII CGCG 4 cut(s) 321, 482, 1090, 1118
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 5 cut(s) 321, 480, 482, 549, 689
AclWI GGATC 5 cut(s) 113, 430, 547, 998, 1057
AcsI RAATTY 2 cut(s) 597, 1099
AcuI CTGAAG 2 cut(s) 240, 563
AdeI CACNNNGTG 1 cut(s) 894
AfiI CCNNNNNNNGG 2 cut(s) 239, 458
AflII CTTAAG 1 cut(s) 7
AflIII ACRYGT 1 cut(s) 1088
AgsI TTSAA 5 cut(s) 118, 178, 257, 356, 977
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 9 cut(s) 91, 224, 278, 608, 647, 989, 1211, 1238, 1276
AluI AGCT 9 cut(s) 91, 224, 278, 608, 647, 989, 1211, 1238, 1276
AlwI GGATC 5 cut(s) 113, 430, 547, 998, 1057
AlwNI CAGNNNCTG 3 cut(s) 658, 844, 1238
Aor13HI TCCGGA 1 cut(s) 332
ApeKI GCWGC 5 cut(s) 263, 266, 569, 608, 953
ApoI RAATTY 2 cut(s) 597, 1099
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 703, 1108
AsuII TTCGAA 1 cut(s) 1257
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 5 cut(s) 250, 253, 556, 595, 940
BccI CCATC 5 cut(s) 136, 284, 366, 619, 788
BciVI GTATCC 1 cut(s) 299
BclI TGATCA 2 cut(s) 45, 66
BcnI CCSGG 1 cut(s) 303
BfmI CTRYAG 2 cut(s) 675, 999
BfrI CTTAAG 1 cut(s) 7
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 6 cut(s) 264, 267, 480, 570, 609, 954
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 6 cut(s) 265, 268, 481, 571, 610, 955
Bme1390I CCNGG 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 5 cut(s) 325, 748, 766, 1064, 1110
BpiI GAAGAC 1 cut(s) 290
BplI GAGNNNNNCTC 4 cut(s) 973, 1005, 1260, 1292
BpmI CTGGAG 2 cut(s) 813, 1191
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1257
BpuMI CCSGG 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 35, 480
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 458
Bse118I RCCGGY 2 cut(s) 458, 821
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 1162
BseAI TCCGGA 1 cut(s) 332
BseCI ATCGAT 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 35, 480
BseGI GGATG 5 cut(s) 59, 120, 295, 377, 674
BseLI CCNNNNNNNGG 2 cut(s) 239, 458
BseMI GCAATG 1 cut(s) 1162
BseMII CTCAG 2 cut(s) 875, 1073
BseNI ACTGG 1 cut(s) 509
BseRI GAGGAG 2 cut(s) 1278, 1281
BseXI GCAGC 5 cut(s) 250, 253, 556, 595, 940
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 4 cut(s) 321, 482, 1090, 1118
BshVI ATCGAT 1 cut(s) 783
BsiSI CCGG 4 cut(s) 303, 333, 459, 822
BslI CCNNNNNNNGG 2 cut(s) 239, 458
BsmI GAATGC 1 cut(s) 462
Bsp119I TTCGAA 1 cut(s) 1257
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp68I TCGCGA 1 cut(s) 1118
BspACI CCGC 5 cut(s) 321, 480, 482, 549, 689
BspCNI CTCAG 2 cut(s) 874, 1072
BspDI ATCGAT 1 cut(s) 783
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 4 cut(s) 321, 482, 1090, 1118
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 2 cut(s) 679, 1003
BspPI GGATC 5 cut(s) 113, 430, 547, 998, 1057
BspT104I TTCGAA 1 cut(s) 1257
BspTI CTTAAG 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 1162
BsrFI RCCGGY 2 cut(s) 458, 821
BsrI ACTGG 1 cut(s) 509
BssAI RCCGGY 2 cut(s) 458, 821
BssECI CCNNGG 2 cut(s) 35, 480
BssMI GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
BssT1I CCWWGG 1 cut(s) 35
Bst4CI ACNGT 6 cut(s) 172, 404, 536, 878, 1136, 1286
Bst6I CTCTTC 1 cut(s) 119
BstAFI CTTAAG 1 cut(s) 7
BstAPI GCANNNNNTGC 1 cut(s) 658
BstBI TTCGAA 1 cut(s) 1257
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 6 cut(s) 225, 467, 648, 806, 861, 1059
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 5 cut(s) 59, 120, 295, 377, 674
BstFNI CGCG 4 cut(s) 321, 482, 1090, 1118
BstKTI GATC 8 cut(s) 48, 69, 108, 425, 542, 803, 1006, 1065
BstMBI GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
BstMWI GCNNNNNNNGC 4 cut(s) 275, 555, 653, 658
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 2 cut(s) 675, 999
BstUI CGCG 4 cut(s) 321, 482, 1090, 1118
BstV1I GCAGC 5 cut(s) 250, 253, 556, 595, 940
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 3 cut(s) 422, 1003, 1062
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 3 cut(s) 422, 1003, 1062
Bsu15I ATCGAT 1 cut(s) 783
BsuI GTATCC 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 783
BtgI CCRYGG 1 cut(s) 480
BtgZI GCGATG 2 cut(s) 302, 1133
BtsCI GGATG 5 cut(s) 59, 120, 295, 377, 674
BtuMI TCGCGA 1 cut(s) 1118
Cac8I GCNNGC 1 cut(s) 654
CaiI CAGNNNCTG 3 cut(s) 658, 844, 1238
Cfr10I RCCGGY 2 cut(s) 458, 821
Cfr42I CCGCGG 1 cut(s) 483
ClaI ATCGAT 1 cut(s) 783
CseI GACGC 2 cut(s) 629, 1096
CviAII CATG 3 cut(s) 64, 125, 1181
DdeI CTNAG 6 cut(s) 225, 467, 648, 806, 861, 1059
DpnI GATC 8 cut(s) 47, 68, 107, 424, 541, 802, 1005, 1064
DpnII GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
DraIII CACNNNGTG 1 cut(s) 894
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 35
Eco57I CTGAAG 2 cut(s) 240, 563
EcoT14I CCWWGG 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 3 cut(s) 67, 128, 1184
FatI CATG 3 cut(s) 63, 124, 1180
FauI CCCGC 2 cut(s) 314, 542
FauNDI CATATG 1 cut(s) 958
FbaI TGATCA 2 cut(s) 45, 66
FblI GTMKAC 1 cut(s) 1030
Fnu4HI GCNGC 6 cut(s) 264, 267, 480, 570, 609, 954
FokI GGATG 5 cut(s) 46, 107, 302, 384, 681
Fsp4HI GCNGC 6 cut(s) 264, 267, 480, 570, 609, 954
GluI GCNGC 6 cut(s) 264, 267, 480, 570, 609, 954
GsuI CTGGAG 2 cut(s) 813, 1191
HapII CCGG 4 cut(s) 303, 333, 459, 822
HgaI GACGC 2 cut(s) 629, 1096
Hin1II CATG 3 cut(s) 67, 128, 1184
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 7 cut(s) 199, 243, 253, 841, 931, 962, 973
HpaII CCGG 4 cut(s) 303, 333, 459, 822
HphI GGTGA 2 cut(s) 703, 1108
Hpy166II GTNNAC 4 cut(s) 168, 400, 874, 1031
Hpy188I TCNGA 5 cut(s) 198, 220, 252, 833, 1062
Hpy188III TCNNGA 8 cut(s) 70, 333, 491, 666, 792, 928, 977, 1117
Hpy8I GTNNAC 4 cut(s) 168, 400, 874, 1031
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 2 cut(s) 741, 866
HpyCH4III ACNGT 6 cut(s) 172, 404, 536, 878, 1136, 1286
HpyCH4V TGCA 9 cut(s) 316, 572, 677, 847, 956, 1001, 1055, 1075, 1232
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 555, 653, 658
HpyF3I CTNAG 6 cut(s) 225, 467, 648, 806, 861, 1059
Hsp92II CATG 3 cut(s) 67, 128, 1184
Kpn2I TCCGGA 1 cut(s) 332
Ksp22I TGATCA 2 cut(s) 45, 66
KspI CCGCGG 1 cut(s) 483
Kzo9I GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
LmnI GCTCC 4 cut(s) 275, 335, 994, 1268
Lsp1109I GCAGC 5 cut(s) 250, 253, 556, 595, 940
LweI GCATC 5 cut(s) 325, 748, 766, 1064, 1110
MaeIII GTNAC 4 cut(s) 11, 530, 1298, 1310
MalI GATC 8 cut(s) 47, 68, 107, 424, 541, 802, 1005, 1064
MboI GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
MboII GAAGA 7 cut(s) 97, 106, 290, 417, 420, 629, 760
MfeI CAATTG 1 cut(s) 343
MflI RGATCY 3 cut(s) 422, 1003, 1062
MluCI AATT 9 cut(s) 343, 356, 391, 597, 719, 1067, 1099, 1147, 1315
MluI ACGCGT 1 cut(s) 1088
MlyI GAGTC 3 cut(s) 193, 237, 925
MmeI TCCRAC 3 cut(s) 83, 220, 998
MroI TCCGGA 1 cut(s) 332
MseI TTAA 5 cut(s) 8, 882, 948, 1035, 1215
MslI CAYNNNNRTG 3 cut(s) 129, 740, 785
MspA1I CMGCKG 1 cut(s) 482
MspCI CTTAAG 1 cut(s) 7
MspI CCGG 4 cut(s) 303, 333, 459, 822
MspR9I CCNGG 1 cut(s) 303
MunI CAATTG 1 cut(s) 343
Mva1269I GAATGC 1 cut(s) 462
MvnI CGCG 4 cut(s) 321, 482, 1090, 1118
MwoI GCNNNNNNNGC 4 cut(s) 275, 555, 653, 658
NciI CCSGG 1 cut(s) 303
NdeI CATATG 1 cut(s) 958
NdeII GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
NlaIII CATG 3 cut(s) 67, 128, 1184
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 4 cut(s) 11, 530, 1298, 1310
NruI TCGCGA 1 cut(s) 1118
NspV TTCGAA 1 cut(s) 1257
OliI CACNNNNGTG 1 cut(s) 740
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 4 cut(s) 253, 841, 962, 973
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 6 cut(s) 265, 268, 481, 571, 610, 955
PleI GAGTC 3 cut(s) 193, 237, 925
PpsI GAGTC 3 cut(s) 193, 237, 925
PsiI TTATAA 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 2 cut(s) 679, 1003
PstNI CAGNNNCTG 3 cut(s) 658, 844, 1238
PsuI RGATCY 3 cut(s) 422, 1003, 1062
RruI TCGCGA 1 cut(s) 1118
RseI CAYNNNNRTG 3 cut(s) 129, 740, 785
SacII CCGCGG 1 cut(s) 483
SaqAI TTAA 5 cut(s) 8, 882, 948, 1035, 1215
SatI GCNGC 6 cut(s) 264, 267, 480, 570, 609, 954
Sau3AI GATC 8 cut(s) 45, 66, 105, 422, 539, 800, 1003, 1062
SchI GAGTC 3 cut(s) 193, 237, 925
ScrFI CCNGG 1 cut(s) 303
SfaNI GCATC 5 cut(s) 325, 748, 766, 1064, 1110
SfcI CTRYAG 2 cut(s) 675, 999
Sfr303I CCGCGG 1 cut(s) 483
SfuI TTCGAA 1 cut(s) 1257
SgrAI CRCCGGYG 1 cut(s) 821
SgrBI CCGCGG 1 cut(s) 483
SmiMI CAYNNNNRTG 3 cut(s) 129, 740, 785
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 9 cut(s) 343, 356, 391, 597, 719, 1067, 1099, 1147, 1315
SsiI CCGC 5 cut(s) 321, 480, 482, 549, 689
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 6 cut(s) 172, 404, 536, 878, 1136, 1286
TaqI TCGA 3 cut(s) 783, 799, 1257
TasI AATT 9 cut(s) 343, 356, 391, 597, 719, 1067, 1099, 1147, 1315
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 4 cut(s) 253, 841, 962, 973
Tru1I TTAA 5 cut(s) 8, 882, 948, 1035, 1215
Tru9I TTAA 5 cut(s) 8, 882, 948, 1035, 1215
TseFI GTSAC 4 cut(s) 11, 530, 1298, 1310
TseI GCWGC 5 cut(s) 263, 266, 569, 608, 953
Tsp45I GTSAC 4 cut(s) 11, 530, 1298, 1310
TspDTI ATGAA 3 cut(s) 98, 168, 420
Vha464I CTTAAG 1 cut(s) 7
XapI RAATTY 2 cut(s) 597, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 1014
XmiI GTMKAC 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.