FvH4_4g21810

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005575
Physical Location & Seq
Reverse (-)
24799861 .. 24804478
4618 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21810.t4

Sequence Viewer

Length: 1242 bp
ATGATGAAGCTGTTCTGGGTTTTTGGGGGGTTTATGGGGTTGATGTTTTGCAGACAAACATATGGTATTGCAGATTGGAATGTGACTCAAGATGAAGACTTGCATTATAGTGAGATTAATGTGTTTGGTTTTAGAAATGGGTTTTCCAGCACACCCTTGATGGTGGGTCTAACTCTCATTAGTGGAGCTGCTGCTAAAGGAGCAGTCTGCCTAGATGGAACATTACCTGCTTACCATATCCACCGGGGATATGGATCAGGTGCAAACAGTTGGCTCATTGACCTGGAGGGAGGAGGATGGTGTGATACAATCAGAAATTGTATCTATCGCAAGAAGACCCGTCGCGGTTCATCAGCATATATGGAGAAGCAGATACCATTCACAGGGATATTGAGCAACAAAGCTAGTGAAAACCCTGACTTTTTCAACTGGAATAGAGTCAAGATTCGATATTGTGATGGTGCATCTTTTGCTGGAGACAGTGAAATTAAGGCAGCACAATTGCATTTTCGAGGGCAACGCATATGGAAAGCTGCTATGGAAGATCTGATGTCCAAGGGAATGCGCTATGCTAATCAGGCTCTTCTTTCTGGATGTTCTGCTGGTGCTGTAGCAACTGTCCTCCACTGTGATGAATTCCGTGAAATGTTTCCTGGAACAACTAGAGTCAAGTGCCTCAGTGATGCTGGATTATTTCTTGATGCATTTGATGTATCTGGTAGCCGCACTTTGAGAAATATGTTTAGACGAGTGGTTAACCTACAGGGAGCAAAGAAGAACCTTCCATGGACTTGTACTAATCGGCTCAACCCAAATTTGCACGTAATTGCAAGTGTCAAGACCCCCCTCTTTCTAGTCAATGCAGCTTATGATACATGGCAGATCCAGGCAAGTTTAGCTCCGCCAACAGCAGATCCTAGTGGCAGATGGAATGAATGCAAAAAGAATCATGCACGTTGCACTTCGCGGCAAATTAACTTTTTTCAAAGCTTCCGGAATCAAATGCTCAAAGCTGTAAGTGGCTTCTCAAGGGCTGGTAAAAATGGTTTGTTCATAAACTCATGTTTTTCGCATTGCCAAACCGAGAGACAGGACACATGGTTTGCTCAAGATTCTCCTCATATTGGAAATAAGGGGATAGCAAAGTCTGTGGGGAACTGGTACTTTGATAGAGTTAATATCAAGGCAATAGATTGTCCTTACCCTTGTGACCACAGCTGCCATAATCTGGTGTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

414

Amino Acids

46.31

Weight (kDa)

9.24

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 293 1.7e-133 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccB7I CCANNNNNTGG 1 cut(s) 1228
AccII CGCG 2 cut(s) 345, 967
AccIII TCCGGA 1 cut(s) 993
AciI CCGC 4 cut(s) 345, 724, 902, 967
AclWI GGATC 3 cut(s) 262, 877, 908
AcsI RAATTY 2 cut(s) 635, 814
AfaI GTAC 2 cut(s) 796, 1163
AfiI CCNNNNNNNGG 3 cut(s) 383, 1124, 1228
AgsI TTSAA 3 cut(s) 427, 986, 1237
AjnI CCWGG 3 cut(s) 282, 652, 885
AluBI AGCT 9 cut(s) 10, 188, 404, 533, 866, 899, 990, 1013, 1218
AluI AGCT 9 cut(s) 10, 188, 404, 533, 866, 899, 990, 1013, 1218
Alw26I GTCTC 2 cut(s) 471, 1081
AlwI GGATC 3 cut(s) 262, 877, 908
Aor13HI TCCGGA 1 cut(s) 993
ApeKI GCWGC 6 cut(s) 188, 191, 494, 533, 863, 1218
ApoI RAATTY 2 cut(s) 635, 814
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
AseI ATTAAT 1 cut(s) 117
Asp700I GAANNNNTTC 2 cut(s) 11, 648
AspLEI GCGC 1 cut(s) 567
AsuC2I CCSGG 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 102, 341
BbvI GCAGC 6 cut(s) 175, 178, 506, 520, 875, 1205
BccI CCATC 5 cut(s) 154, 209, 291, 452, 921
BciT130I CCWGG 3 cut(s) 284, 654, 887
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 471, 1081
BfaI CTAG 5 cut(s) 212, 405, 663, 854, 918
BfmI CTRYAG 2 cut(s) 609, 761
BfuAI ACCTGC 1 cut(s) 235
BglII AGATCT 1 cut(s) 544
BisI GCNGC 8 cut(s) 189, 192, 495, 534, 724, 864, 968, 1219
BlsI GCNGC 8 cut(s) 190, 193, 496, 535, 725, 865, 969, 1220
Bme1390I CCNGG 4 cut(s) 245, 284, 654, 887
BmrFI CCNGG 4 cut(s) 245, 284, 654, 887
BmsI GCATC 3 cut(s) 473, 673, 691
BpiI GAAGAC 2 cut(s) 102, 341
BpmI CTGGAG 2 cut(s) 305, 495
BpuEI CTTGAG 3 cut(s) 72, 1012, 1092
BpuMI CCSGG 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 823
BsaBI GATNNNNATC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 244, 555, 785
BsaWI WCCGGW 1 cut(s) 993
Bsc4I CCNNNNNNNGG 3 cut(s) 383, 1124, 1228
Bse1I ACTGG 2 cut(s) 434, 1163
Bse3DI GCAATG 1 cut(s) 1072
Bse8I GATNNNNATC 1 cut(s) 253
BseAI TCCGGA 1 cut(s) 993
BseBI CCWGG 3 cut(s) 284, 654, 887
BseDI CCNNGG 3 cut(s) 244, 555, 785
BseGI GGATG 2 cut(s) 302, 599
BseJI GATNNNNATC 1 cut(s) 253
BseLI CCNNNNNNNGG 3 cut(s) 383, 1124, 1228
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 1 cut(s) 691
BseNI ACTGG 2 cut(s) 434, 1163
BseRI GAGGAG 2 cut(s) 306, 1107
BseXI GCAGC 6 cut(s) 175, 178, 506, 520, 875, 1205
Bsh1236I CGCG 2 cut(s) 345, 967
BsiSI CCGG 2 cut(s) 244, 994
BslI CCNNNNNNNGG 3 cut(s) 383, 1124, 1228
BsmAI GTCTC 2 cut(s) 471, 1081
BsmI GAATGC 2 cut(s) 567, 941
Bsp13I TCCGGA 1 cut(s) 993
Bsp143I GATC 4 cut(s) 254, 544, 882, 913
Bsp19I CCATGG 1 cut(s) 785
BspACI CCGC 4 cut(s) 345, 724, 902, 967
BspCNI CTCAG 1 cut(s) 690
BspEI TCCGGA 1 cut(s) 993
BspFNI CGCG 2 cut(s) 345, 967
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 3 cut(s) 262, 877, 908
BspQI GCTCTTC 1 cut(s) 588
BsrDI GCAATG 1 cut(s) 1072
BsrI ACTGG 2 cut(s) 434, 1163
BssECI CCNNGG 3 cut(s) 244, 555, 785
BssMI GATC 4 cut(s) 254, 544, 882, 913
BssT1I CCWWGG 2 cut(s) 555, 785
Bst2UI CCWGG 3 cut(s) 284, 654, 887
Bst4CI ACNGT 4 cut(s) 269, 482, 619, 629
Bst6I CTCTTC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 823
BstDEI CTNAG 1 cut(s) 677
BstDSI CCRYGG 1 cut(s) 785
BstF5I GGATG 2 cut(s) 302, 599
BstFNI CGCG 2 cut(s) 345, 967
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 4 cut(s) 257, 547, 885, 916
BstMAI GTCTC 2 cut(s) 471, 1081
BstMBI GATC 4 cut(s) 254, 544, 882, 913
BstMWI GCNNNNNNNGC 4 cut(s) 200, 470, 578, 896
BstNI CCWGG 3 cut(s) 284, 654, 887
BstSCI CCNGG 4 cut(s) 243, 282, 652, 885
BstSFI CTRYAG 2 cut(s) 609, 761
BstUI CGCG 2 cut(s) 345, 967
BstV1I GCAGC 6 cut(s) 175, 178, 506, 520, 875, 1205
BstV2I GAAGAC 2 cut(s) 102, 341
BstX2I RGATCY 3 cut(s) 544, 882, 913
BstYI RGATCY 3 cut(s) 544, 882, 913
BtgI CCRYGG 1 cut(s) 785
BtsCI GGATG 2 cut(s) 302, 599
BtsIMutI CAGTG 3 cut(s) 487, 625, 685
BveI ACCTGC 1 cut(s) 235
CfoI GCGC 1 cut(s) 567
Csp6I GTAC 2 cut(s) 795, 1162
CspCI CAANNNNNGTGG 2 cut(s) 1131, 1166
CviAII CATG 5 cut(s) 786, 876, 950, 1062, 1098
CviQI GTAC 2 cut(s) 795, 1162
DdeI CTNAG 1 cut(s) 677
DpnI GATC 4 cut(s) 256, 546, 884, 915
DpnII GATC 4 cut(s) 254, 544, 882, 913
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
EciI GGCGGA 1 cut(s) 891
Eco130I CCWWGG 2 cut(s) 555, 785
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 3 cut(s) 282, 652, 885
EcoT14I CCWWGG 2 cut(s) 555, 785
EcoT22I ATGCAT 1 cut(s) 706
ErhI CCWWGG 2 cut(s) 555, 785
FaeI CATG 5 cut(s) 789, 879, 953, 1065, 1101
FatI CATG 5 cut(s) 785, 875, 949, 1061, 1097
FauNDI CATATG 2 cut(s) 61, 524
Fnu4HI GCNGC 8 cut(s) 189, 192, 495, 534, 724, 864, 968, 1219
FokI GGATG 2 cut(s) 309, 606
Fsp4HI GCNGC 8 cut(s) 189, 192, 495, 534, 724, 864, 968, 1219
FspBI CTAG 5 cut(s) 212, 405, 663, 854, 918
GlaI GCGC 1 cut(s) 566
GluI GCNGC 8 cut(s) 189, 192, 495, 534, 724, 864, 968, 1219
GsuI CTGGAG 2 cut(s) 305, 495
HapII CCGG 2 cut(s) 244, 994
HhaI GCGC 1 cut(s) 567
Hin1II CATG 5 cut(s) 789, 879, 953, 1065, 1101
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 1 cut(s) 988
HinfI GANTC 7 cut(s) 85, 438, 445, 666, 946, 997, 1112
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 2 cut(s) 244, 994
Hpy166II GTNNAC 1 cut(s) 757
Hpy188I TCNGA 2 cut(s) 314, 549
Hpy188III TCNNGA 7 cut(s) 89, 442, 591, 698, 838, 994, 1109
Hpy8I GTNNAC 1 cut(s) 757
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 1 cut(s) 791
HpyCH4III ACNGT 4 cut(s) 269, 482, 619, 629
HpyCH4IV ACGT 2 cut(s) 822, 955
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 470, 578, 896
HpyF3I CTNAG 1 cut(s) 677
HpySE526I ACGT 2 cut(s) 822, 955
Hsp92II CATG 5 cut(s) 789, 879, 953, 1065, 1101
HspAI GCGC 1 cut(s) 565
Kpn2I TCCGGA 1 cut(s) 993
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 4 cut(s) 254, 544, 882, 913
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 4 cut(s) 185, 200, 767, 904
Lsp1109I GCAGC 6 cut(s) 175, 178, 506, 520, 875, 1205
LweI GCATC 3 cut(s) 473, 673, 691
MaeI CTAG 5 cut(s) 212, 405, 663, 854, 918
MaeII ACGT 2 cut(s) 822, 955
MaeIII GTNAC 2 cut(s) 82, 1208
MalI GATC 4 cut(s) 256, 546, 884, 915
MboI GATC 4 cut(s) 254, 544, 882, 913
MboII GAAGA 5 cut(s) 107, 346, 554, 575, 787
MfeI CAATTG 1 cut(s) 500
MflI RGATCY 3 cut(s) 544, 882, 913
MluCI AATT 7 cut(s) 316, 486, 500, 635, 814, 825, 972
MlyI GAGTC 3 cut(s) 79, 447, 675
MnlI CCTC 8 cut(s) 280, 284, 287, 506, 632, 686, 857, 1128
Mph1103I ATGCAT 1 cut(s) 706
MroI TCCGGA 1 cut(s) 993
MroXI GAANNNNTTC 2 cut(s) 11, 648
MseI TTAA 5 cut(s) 117, 489, 756, 975, 1176
MslI CAYNNNNRTG 2 cut(s) 108, 630
MspA1I CMGCKG 1 cut(s) 1218
MspI CCGG 2 cut(s) 244, 994
MspR9I CCNGG 4 cut(s) 245, 284, 654, 887
MunI CAATTG 1 cut(s) 500
Mva1269I GAATGC 2 cut(s) 567, 941
MvaI CCWGG 3 cut(s) 284, 654, 887
MvnI CGCG 2 cut(s) 345, 967
MwoI GCNNNNNNNGC 4 cut(s) 200, 470, 578, 896
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 1 cut(s) 785
NdeI CATATG 2 cut(s) 61, 524
NdeII GATC 4 cut(s) 254, 544, 882, 913
NlaIII CATG 5 cut(s) 789, 879, 953, 1065, 1101
NmuCI GTSAC 2 cut(s) 82, 1208
NsiI ATGCAT 1 cut(s) 706
PciSI GCTCTTC 1 cut(s) 588
PctI GAATGC 2 cut(s) 567, 941
PdmI GAANNNNTTC 2 cut(s) 11, 648
PfeI GAWTC 4 cut(s) 445, 946, 997, 1112
PflMI CCANNNNNTGG 1 cut(s) 1228
PfoI TCCNGGA 1 cut(s) 652
PkrI GCNGC 8 cut(s) 190, 193, 496, 535, 725, 865, 969, 1220
PleI GAGTC 3 cut(s) 79, 446, 674
PpsI GAGTC 3 cut(s) 79, 446, 674
Ppu21I YACGTR 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 117
Psp6I CCWGG 3 cut(s) 282, 652, 885
PspGI CCWGG 3 cut(s) 282, 652, 885
PsuI RGATCY 3 cut(s) 544, 882, 913
PvuII CAGCTG 1 cut(s) 1218
RsaI GTAC 2 cut(s) 796, 1163
RsaNI GTAC 2 cut(s) 795, 1162
RseI CAYNNNNRTG 2 cut(s) 108, 630
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 5 cut(s) 117, 489, 756, 975, 1176
SatI GCNGC 8 cut(s) 189, 192, 495, 534, 724, 864, 968, 1219
Sau3AI GATC 4 cut(s) 254, 544, 882, 913
SchI GAGTC 3 cut(s) 79, 447, 675
ScrFI CCNGG 4 cut(s) 245, 284, 654, 887
SfaNI GCATC 3 cut(s) 473, 673, 691
SfcI CTRYAG 2 cut(s) 609, 761
SmiMI CAYNNNNRTG 2 cut(s) 108, 630
SmlI CTYRAG 3 cut(s) 87, 1027, 1107
SmoI CTYRAG 3 cut(s) 87, 1027, 1107
Sse9I AATT 7 cut(s) 316, 486, 500, 635, 814, 825, 972
SsiI CCGC 4 cut(s) 345, 724, 902, 967
SspMI CTAG 5 cut(s) 212, 405, 663, 854, 918
StyD4I CCNGG 4 cut(s) 243, 282, 652, 885
StyI CCWWGG 2 cut(s) 555, 785
TaaI ACNGT 4 cut(s) 269, 482, 619, 629
TaiI ACGT 2 cut(s) 825, 958
TaqI TCGA 2 cut(s) 448, 511
TasI AATT 7 cut(s) 316, 486, 500, 635, 814, 825, 972
TatI WGTACW 1 cut(s) 794
TauI GCSGC 2 cut(s) 726, 970
TfiI GAWTC 4 cut(s) 445, 946, 997, 1112
Tru1I TTAA 5 cut(s) 117, 489, 756, 975, 1176
Tru9I TTAA 5 cut(s) 117, 489, 756, 975, 1176
TscAI CASTG 3 cut(s) 487, 632, 685
TseFI GTSAC 2 cut(s) 82, 1208
TseI GCWGC 6 cut(s) 188, 191, 494, 533, 863, 1218
Tsp45I GTSAC 2 cut(s) 82, 1208
TspDTI ATGAA 6 cut(s) 20, 108, 339, 648, 948, 1042
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 3 cut(s) 487, 632, 685
Van91I CCANNNNNTGG 1 cut(s) 1228
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 2 cut(s) 635, 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmnI GAANNNNTTC 2 cut(s) 11, 648
XspI CTAG 5 cut(s) 212, 405, 663, 854, 918
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.