AT1G10070

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008582
Physical Location & Seq
Forward (+)
3288087 .. 3290471
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10070.3

Sequence Viewer

Length: 1167 bp
ATGATCAAAACAATCACATCTCTACGCAAAACTCTGGTTCTACCTCTTCATTTACATATTCGTACGCTACAAACTTTCGCCAAGTACAACGCACAAGCTGCATCGGCTTTGCGAGAAGAGCGTAAGAAACCTCTTTATCAAAATGGAGATGATGTATATGCGGATTTGGATTGGGATAATCTCGGGTTTGGTCTAAATCCAGCTGATTACATGTATGTCATGAAATGCTCAAAAGACGGCGAATTCACTCAAGGAGAACTTAGTCCCTATGGGAATATTCAGCTAAGTCCTTCTGCTGGAGTCTTAAACTATGGACAGGCGATATACGAAGGTACAAAAGCATACAGGAAAGAAAATGGGAAGCTTCTTTTGTTTCGTCCGGATCACAACGCTATCCGGATGAAGCTTGGCGCTGAACGGATGCTCATGCCTTCTCCTTCGGTTGATCAGTTTGTTAATGCAGTTAAACAAACCGCTCTTGCAAACAAACGTTGGGTTCCTCCTGCAGGGAAAGGGACTTTGTACATTAGGCCTTTGTTGATGGGAAGTGGTCCAATACTTGGTTTAGGTCCTGCACCTGAATATACATTCATTGTCTATGCATCTCCAGTTGGTAACTACTTCAAGGAAGGGATGGCTGCTCTTAACCTCTATGTTGAGGAAGAATATGTCCGAGCGGCTCCTGGTGGAGCTGGAGGCGTCAAGAGCATCACAAATTATGCGCCAGTTTTGAAAGCACTGAGCAGAGCCAAGAGTCGGGGGTTTTCAGACGTTCTTTATCTCGACTCTGTCAAGAAGAAGTACTTAGAGGAGGCTTCTTCTTGCAACGTCTTTGTTGTCAAGGGTCGGACAATCTCAACTCCTGCAACTAATGGAACAATTCTTGAAGGGATTACGCGGAAAAGTGTGATGGAGATCGCAAGTGATCAAGGTTATCAGGTAGTAGAGAAGGCAGTTCATGTGGATGAAGTAATGGATGCAGATGAAGTTTTTTGCACCGGAACTGCTGTAGTAGTTGCTCCCGTGGGCACTATCACATATCAGGAAAAAAGAGTAGAGTATAAAACCGGGGATGAATCTGTCTGCCAGAAACTGCGTTCAGTCCTCGTAGGTATCCAGACAGGATTGATTGAAGATAACAAGGGATGGGTCACAGATATCAACTGA

Protein Analysis

388

Amino Acids

42.59

Weight (kDa)

8.6

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 38 - 273 5.7e-32 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 560
AccBSI CCGCTC 2 cut(s) 476, 677
AccII CGCG 1 cut(s) 898
AccIII TCCGGA 2 cut(s) 379, 396
AciI CCGC 4 cut(s) 161, 474, 677, 898
AclI AACGTT 1 cut(s) 490
AclWI GGATC 1 cut(s) 390
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 699
AfaI GTAC 5 cut(s) 64, 86, 334, 524, 803
AfiI CCNNNNNNNGG 4 cut(s) 296, 506, 560, 756
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 4 cut(s) 625, 733, 887, 1133
AjnI CCWGG 1 cut(s) 682
AluBI AGCT 6 cut(s) 98, 203, 283, 364, 406, 692
AluI AGCT 6 cut(s) 98, 203, 283, 364, 406, 692
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 1093
Ama87I CYCGRG 1 cut(s) 182
Aor13HI TCCGGA 2 cut(s) 379, 396
AoxI GGCC 1 cut(s) 530
ApeKI GCWGC 2 cut(s) 98, 638
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 2 cut(s) 413, 724
AspS9I GGNCC 2 cut(s) 551, 569
AsuC2I CCSGG 1 cut(s) 1069
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 2 cut(s) 551, 569
BaeGI GKGCMC 1 cut(s) 1031
BbvI GCAGC 2 cut(s) 85, 625
BccI CCATC 4 cut(s) 535, 628, 904, 1140
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 1124
BclI TGATCA 3 cut(s) 3, 445, 925
BcnI CCSGG 1 cut(s) 1069
BfmI CTRYAG 2 cut(s) 504, 1008
BfoI RGCGCY 1 cut(s) 414
BfuI GTATCC 1 cut(s) 1124
BisI GCNGC 3 cut(s) 99, 639, 678
BlsI GCNGC 3 cut(s) 100, 640, 679
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 2 cut(s) 684, 1069
Bme18I GGWCC 2 cut(s) 551, 569
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 551, 569
BmiI GGNNCC 2 cut(s) 498, 681
BmrFI CCNGG 2 cut(s) 684, 1069
BmsI GCATC 5 cut(s) 110, 411, 611, 717, 967
BpmI CTGGAG 3 cut(s) 318, 591, 714
BpuEI CTTGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 1069
BsaBI GATNNNNATC 1 cut(s) 914
BsaHI GRCGYC 1 cut(s) 699
BsaJI CCNNGG 2 cut(s) 1023, 1068
BsaWI WCCGGW 3 cut(s) 379, 396, 998
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 506, 560, 756
Bse1I ACTGG 2 cut(s) 608, 725
Bse8I GATNNNNATC 1 cut(s) 914
BseAI TCCGGA 2 cut(s) 379, 396
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 2 cut(s) 1023, 1068
BseGI GGATG 7 cut(s) 405, 426, 639, 970, 982, 1078, 1151
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 4 cut(s) 296, 506, 560, 756
BseMII CTCAG 1 cut(s) 731
BseNI ACTGG 2 cut(s) 608, 725
BseRI GAGGAG 1 cut(s) 824
BseSI GKGCMC 1 cut(s) 1031
BseXI GCAGC 2 cut(s) 85, 625
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 898
BshFI GGCC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 4 cut(s) 380, 397, 999, 1068
BsiWI CGTACG 1 cut(s) 62
BslFI GGGAC 2 cut(s) 249, 529
BslI CCNNNNNNNGG 4 cut(s) 296, 506, 560, 756
BsmFI GGGAC 2 cut(s) 249, 529
BsnI GGCC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 1031
Bsp13I TCCGGA 2 cut(s) 379, 396
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 5 cut(s) 3, 382, 445, 915, 925
BspACI CCGC 4 cut(s) 161, 474, 677, 898
BspANI GGCC 1 cut(s) 532
BspCNI CTCAG 1 cut(s) 732
BspEI TCCGGA 2 cut(s) 379, 396
BspFNI CGCG 1 cut(s) 898
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 498, 681
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 1 cut(s) 111
BsrBI CCGCTC 2 cut(s) 476, 677
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 2 cut(s) 608, 725
BssECI CCNNGG 2 cut(s) 1023, 1068
BssMI GATC 5 cut(s) 3, 382, 445, 915, 925
BssNI GRCGYC 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 684
Bst6I CTCTTC 2 cut(s) 51, 111
BstACI GRCGYC 1 cut(s) 699
BstAPI GCANNNNNTGC 1 cut(s) 98
BstAUI TGTACA 1 cut(s) 522
BstDEI CTNAG 4 cut(s) 260, 284, 740, 805
BstDSI CCRYGG 1 cut(s) 1023
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 7 cut(s) 405, 426, 639, 970, 982, 1078, 1151
BstFNI CGCG 1 cut(s) 898
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 2 cut(s) 413, 724
BstKTI GATC 5 cut(s) 6, 385, 448, 918, 928
BstMBI GATC 5 cut(s) 3, 382, 445, 915, 925
BstMWI GCNNNNNNNGC 4 cut(s) 98, 104, 118, 705
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 682, 1067
BstSFI CTRYAG 2 cut(s) 504, 1008
BstSLI GKGCMC 1 cut(s) 1031
BstUI CGCG 1 cut(s) 898
BstV1I GCAGC 2 cut(s) 85, 625
BsuI GTATCC 1 cut(s) 1124
BsuRI GGCC 1 cut(s) 532
BtgI CCRYGG 1 cut(s) 1023
BtsCI GGATG 7 cut(s) 405, 426, 639, 970, 982, 1078, 1151
BtsIMutI CAGTG 1 cut(s) 737
CaiI CAGNNNCTG 1 cut(s) 1093
CciI TCATGA 1 cut(s) 219
CfoI GCGC 2 cut(s) 413, 724
Cfr13I GGNCC 2 cut(s) 551, 569
CseI GACGC 1 cut(s) 688
Csp6I GTAC 5 cut(s) 63, 85, 333, 523, 802
CviAII CATG 4 cut(s) 211, 220, 427, 959
CviQI GTAC 5 cut(s) 63, 85, 333, 523, 802
DdeI CTNAG 4 cut(s) 260, 284, 740, 805
DpnI GATC 5 cut(s) 5, 384, 447, 917, 927
DpnII GATC 5 cut(s) 3, 382, 445, 915, 925
Eam1104I CTCTTC 2 cut(s) 51, 111
EarI CTCTTC 2 cut(s) 51, 111
Eco147I AGGCCT 1 cut(s) 532
Eco32I GATATC 1 cut(s) 1159
Eco47I GGWCC 2 cut(s) 551, 569
Eco88I CYCGRG 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 569
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 1159
EcoT22I ATGCAT 1 cut(s) 604
FaeI CATG 4 cut(s) 214, 223, 430, 962
FalI AAGNNNNNCTT 4 cut(s) 243, 275, 788, 820
FaqI GGGAC 2 cut(s) 249, 529
FatI CATG 4 cut(s) 210, 219, 426, 958
FbaI TGATCA 3 cut(s) 3, 445, 925
Fnu4HI GCNGC 3 cut(s) 99, 639, 678
FokI GGATG 7 cut(s) 412, 433, 646, 977, 989, 1085, 1158
Fsp4HI GCNGC 3 cut(s) 99, 639, 678
GlaI GCGC 2 cut(s) 412, 723
GluI GCNGC 3 cut(s) 99, 639, 678
GsuI CTGGAG 3 cut(s) 318, 591, 714
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 1 cut(s) 532
HapII CCGG 4 cut(s) 380, 397, 999, 1068
HgaI GACGC 1 cut(s) 688
HhaI GCGC 2 cut(s) 413, 724
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 4 cut(s) 214, 223, 430, 962
Hin6I GCGC 2 cut(s) 411, 722
HinP1I GCGC 2 cut(s) 411, 722
HindIII AAGCTT 2 cut(s) 362, 404
HinfI GANTC 4 cut(s) 300, 754, 785, 1076
HpaII CCGG 4 cut(s) 380, 397, 999, 1068
Hpy188I TCNGA 3 cut(s) 674, 769, 849
Hpy188III TCNNGA 9 cut(s) 220, 380, 397, 703, 782, 793, 884, 1043, 1117
HpyAV CCTTC 7 cut(s) 300, 323, 441, 447, 623, 881, 943
HpyCH4IV ACGT 3 cut(s) 490, 771, 828
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 104, 118, 705
HpyF3I CTNAG 4 cut(s) 260, 284, 740, 805
HpySE526I ACGT 3 cut(s) 490, 771, 828
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 4 cut(s) 214, 223, 430, 962
HspAI GCGC 2 cut(s) 411, 722
Kpn2I TCCGGA 2 cut(s) 379, 396
Ksp22I TGATCA 3 cut(s) 3, 445, 925
Kzo9I GATC 5 cut(s) 3, 382, 445, 915, 925
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 3 cut(s) 685, 689, 1024
Lsp1109I GCAGC 2 cut(s) 85, 625
LweI GCATC 5 cut(s) 110, 411, 611, 717, 967
MaeII ACGT 3 cut(s) 490, 771, 828
MaeIII GTNAC 2 cut(s) 614, 1150
MalI GATC 5 cut(s) 5, 384, 447, 917, 927
MbiI CCGCTC 2 cut(s) 476, 677
MboI GATC 5 cut(s) 3, 382, 445, 915, 925
MboII GAAGA 6 cut(s) 38, 128, 674, 808, 810, 1145
MhlI GDGCHC 1 cut(s) 1031
MluCI AATT 3 cut(s) 242, 715, 879
MlyI GAGTC 3 cut(s) 309, 763, 779
MmeI TCCRAC 1 cut(s) 827
MnlI CCTC 9 cut(s) 54, 141, 510, 652, 659, 689, 802, 805, 1115
Mph1103I ATGCAT 1 cut(s) 604
MroI TCCGGA 2 cut(s) 379, 396
MseI TTAA 4 cut(s) 305, 456, 465, 645
MslI CAYNNNNRTG 1 cut(s) 963
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 4 cut(s) 380, 397, 999, 1068
MspR9I CCNGG 2 cut(s) 684, 1069
MvaI CCWGG 1 cut(s) 684
MvnI CGCG 1 cut(s) 898
MwoI GCNNNNNNNGC 4 cut(s) 98, 104, 118, 705
NciI CCSGG 1 cut(s) 1069
NdeII GATC 5 cut(s) 3, 382, 445, 915, 925
NlaIII CATG 4 cut(s) 214, 223, 430, 962
NlaIV GGNNCC 2 cut(s) 498, 681
NmuCI GTSAC 1 cut(s) 1150
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 214
PagI TCATGA 1 cut(s) 219
PceI AGGCCT 1 cut(s) 532
PciI ACATGT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 1076
Pfl23II CGTACG 1 cut(s) 62
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 1 cut(s) 560
PkrI GCNGC 3 cut(s) 100, 640, 679
PleI GAGTC 3 cut(s) 308, 762, 779
PpsI GAGTC 3 cut(s) 308, 762, 779
PpuMI RGGWCCY 1 cut(s) 569
PscI ACATGT 1 cut(s) 210
Psp1406I AACGTT 1 cut(s) 490
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspLI CGTACG 1 cut(s) 62
PspN4I GGNNCC 2 cut(s) 498, 681
PspPI GGNCC 2 cut(s) 551, 569
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 1093
PsyI GACNNNGTC 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 5 cut(s) 64, 86, 334, 524, 803
RsaNI GTAC 5 cut(s) 63, 85, 333, 523, 802
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 4 cut(s) 305, 456, 465, 645
SatI GCNGC 3 cut(s) 99, 639, 678
Sau3AI GATC 5 cut(s) 3, 382, 445, 915, 925
Sau96I GGNCC 2 cut(s) 551, 569
SbfI CCTGCAGG 1 cut(s) 508
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 3 cut(s) 309, 763, 779
ScrFI CCNGG 2 cut(s) 684, 1069
SdaI CCTGCAGG 1 cut(s) 508
SduI GDGCHC 1 cut(s) 1031
SfaNI GCATC 5 cut(s) 110, 411, 611, 717, 967
SfcI CTRYAG 2 cut(s) 504, 1008
SinI GGWCC 2 cut(s) 551, 569
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse8387I CCTGCAGG 1 cut(s) 508
Sse9I AATT 3 cut(s) 242, 715, 879
SseBI AGGCCT 1 cut(s) 532
SsiI CCGC 4 cut(s) 161, 474, 677, 898
SspI AATATT 1 cut(s) 277
StuI AGGCCT 1 cut(s) 532
StyD4I CCNGG 2 cut(s) 682, 1067
TaiI ACGT 3 cut(s) 493, 774, 831
TaqI TCGA 1 cut(s) 783
TasI AATT 3 cut(s) 242, 715, 879
TatI WGTACW 3 cut(s) 84, 522, 801
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 1 cut(s) 1076
Tru1I TTAA 4 cut(s) 305, 456, 465, 645
Tru9I TTAA 4 cut(s) 305, 456, 465, 645
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 1 cut(s) 1150
TseI GCWGC 2 cut(s) 98, 638
Tsp45I GTSAC 1 cut(s) 1150
TspDTI ATGAA 8 cut(s) 38, 236, 416, 580, 947, 981, 999, 1089
TspGWI ACGGA 1 cut(s) 433
TspRI CASTG 1 cut(s) 744
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 551, 569
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 242
XceI RCATGY 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.