Rh3BG088200

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008582
Physical Location & Seq
Reverse (-)
6883311 .. 6887638
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG088200.1

Sequence Viewer

Length: 834 bp
ATGAAAGCATTCAGGAATGAAGATGGCAATCTCCTTATCTTTCGTCCAGACCAGAATGCAATGCGAATGAAGATTGGTTCAGAAAGAATGTGCATGCCCTCGCCATCCCTTGATCAATTTATCGATGCTGTGAAGCAAACTGCTCAAGCTAACAAGTGTTGGGTTCCCCCTCCAGGAAAAGGGTCTTTATATATTAGGCCTCTACTTATAGGAAGTGGTCCTATATTAGGTTTAGGCACGTCGCCTGAATACACATTCCTTGTATACGCTTCACCAGTCCGCAACTATTTCAAGGAGGGTTCTGCACCTTTGAGCATATACGTCGAGGAGGAATATGATCGTTCCTCTCGTGGTGGGGCTGGAGGTGTCAAAAGCATCACCAATTATGCTCCAGTTTTGAAGGCACTACTCAGAGCAAAGAGCCGAGGATTTTCTGATGTGTTATATCTAGACTCGGTAAATAAAAAGAATCTGGAGGAGGTCTCTTCTTGTAACATTTTCATTGTGAAGGGCAATGTTATTTCAACTCCTGCAACGGCTGGGACTATACTATCAGGGGTAACTCGAAGAAGCATCATTGAAATTGCTCGCGATCATGGTTATCAGGTTGAGGAGCGTGCAATTCCGGTAGATGAACTGAGTGAAGCTGATGAAGTATTCTGTACAGGAACTGCAGTCGGTGTTGCTCCTGTGGGAAACATTACATATAAAGATAAAAGGATGGAATACAAAACAGGTGCTCAAACTGTTTGTCCACAGCTCTACTCAACTCTCATAGGAATTCAAACGGGTCGTATTGAGGATAAGAAAGGCTGGATTCTTGAAATTGATTGA

Protein Analysis

277

Amino Acids

30.36

Weight (kDa)

8.17

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 9 - 234 4.2e-35 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 264
AccII CGCG 1 cut(s) 591
AciI CCGC 1 cut(s) 280
AcsI RAATTY 1 cut(s) 780
AfaI GTAC 1 cut(s) 664
AfiI CCNNNNNNNGG 3 cut(s) 173, 179, 227
AgsI TTSAA 6 cut(s) 292, 400, 525, 581, 785, 824
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 3 cut(s) 149, 647, 760
AluI AGCT 3 cut(s) 149, 647, 760
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 1 cut(s) 487
AlwNI CAGNNNCTG 1 cut(s) 671
AoxI GGCC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 2 cut(s) 736, 768
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 264, 370
AvaII GGWCC 1 cut(s) 218
BauI CACGAG 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 742
BccI CCATC 3 cut(s) 17, 112, 715
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 1 cut(s) 174
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 672
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 115, 384, 582
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpmI CTGGAG 4 cut(s) 156, 375, 381, 494
BpuEI CTTGAG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 424
BsaWI WCCGGW 1 cut(s) 625
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 179, 227
Bse1I ACTGG 2 cut(s) 275, 392
Bse3DI GCAATG 2 cut(s) 66, 520
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 1 cut(s) 174
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 2 cut(s) 104, 726
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 173, 179, 227
BseMI GCAATG 2 cut(s) 66, 520
BseMII CTCAG 2 cut(s) 424, 629
BseNI ACTGG 2 cut(s) 275, 392
BseRI GAGGAG 3 cut(s) 341, 491, 626
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 591
BshFI GGCC 1 cut(s) 199
BshVI ATCGAT 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 173, 179, 227
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 556
BsmI GAATGC 2 cut(s) 8, 61
BsnI GGCC 1 cut(s) 199
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 662
Bsp143I GATC 3 cut(s) 112, 337, 592
Bsp68I TCGCGA 1 cut(s) 591
BspACI CCGC 1 cut(s) 280
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 2 cut(s) 423, 630
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 1 cut(s) 591
BspLI GGNNCC 1 cut(s) 165
BspMAI CTGCAG 1 cut(s) 676
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 2 cut(s) 66, 520
BsrGI TGTACA 1 cut(s) 662
BsrI ACTGG 2 cut(s) 275, 392
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 3 cut(s) 112, 337, 592
BssNAI GTATAC 1 cut(s) 265
BssSI CACGAG 1 cut(s) 348
Bst1107I GTATAC 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 748
Bst6I CTCTTC 1 cut(s) 490
BstAUI TGTACA 1 cut(s) 662
BstC8I GCNNGC 3 cut(s) 95, 589, 618
BstDEI CTNAG 2 cut(s) 410, 638
BstENI CCTNNNNNAGG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 104, 726
BstFNI CGCG 1 cut(s) 591
BstKTI GATC 3 cut(s) 115, 340, 595
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 3 cut(s) 112, 337, 592
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 1 cut(s) 591
BstZ17I GTATAC 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 199
BsuTUI ATCGAT 1 cut(s) 123
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 2 cut(s) 104, 726
BtuMI TCGCGA 1 cut(s) 591
Cac8I GCNNGC 3 cut(s) 95, 589, 618
CaiI CAGNNNCTG 1 cut(s) 671
Cfr13I GGNCC 1 cut(s) 218
ClaI ATCGAT 1 cut(s) 123
Csp6I GTAC 1 cut(s) 663
CviAII CATG 2 cut(s) 94, 596
CviJI RGCY 8 cut(s) 149, 199, 359, 423, 539, 647, 760, 813
CviKI_1 RGCY 8 cut(s) 149, 199, 359, 423, 539, 647, 760, 813
CviQI GTAC 1 cut(s) 663
DdeI CTNAG 2 cut(s) 410, 638
DpnI GATC 3 cut(s) 114, 339, 594
DpnII GATC 3 cut(s) 112, 337, 592
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
Eco147I AGGCCT 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 225
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 172
FaeI CATG 2 cut(s) 97, 599
FaqI GGGAC 1 cut(s) 556
FatI CATG 2 cut(s) 93, 595
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 264
FokI GGATG 2 cut(s) 91, 733
FspBI CTAG 1 cut(s) 449
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 4 cut(s) 156, 375, 381, 494
HaeIII GGCC 1 cut(s) 199
HapII CCGG 1 cut(s) 626
Hin1II CATG 2 cut(s) 97, 599
HinfI GANTC 3 cut(s) 452, 469, 817
HpaII CCGG 1 cut(s) 626
HphI GGTGA 2 cut(s) 264, 370
Hpy166II GTNNAC 2 cut(s) 265, 755
Hpy188I TCNGA 3 cut(s) 82, 413, 436
Hpy188III TCNNGA 6 cut(s) 13, 47, 449, 473, 590, 821
Hpy8I GTNNAC 2 cut(s) 265, 755
Hpy99I CGWCG 2 cut(s) 244, 326
HpyAV CCTTC 2 cut(s) 394, 502
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 2 cut(s) 239, 321
HpyCH4V TGCA 6 cut(s) 59, 93, 305, 533, 620, 674
HpyF3I CTNAG 2 cut(s) 410, 638
HpySE526I ACGT 2 cut(s) 239, 321
Hsp92II CATG 2 cut(s) 97, 599
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 3 cut(s) 112, 337, 592
LmnI GCTCC 3 cut(s) 394, 613, 691
LweI GCATC 3 cut(s) 115, 384, 582
MaeI CTAG 1 cut(s) 449
MaeII ACGT 2 cut(s) 239, 321
MaeIII GTNAC 2 cut(s) 491, 559
MalI GATC 3 cut(s) 114, 339, 594
MboI GATC 3 cut(s) 112, 337, 592
MboII GAAGA 4 cut(s) 32, 82, 477, 579
MhlI GDGCHC 1 cut(s) 742
MluCI AATT 6 cut(s) 116, 382, 582, 621, 780, 825
MlyI GAGTC 1 cut(s) 446
MroXI GAANNNNTTC 1 cut(s) 8
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 1 cut(s) 174
Mva1269I GAATGC 2 cut(s) 8, 61
MvaI CCWGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 591
NdeII GATC 3 cut(s) 112, 337, 592
NlaIII CATG 2 cut(s) 97, 599
NlaIV GGNNCC 1 cut(s) 165
NmeAIII GCCGAG 1 cut(s) 449
NruI TCGCGA 1 cut(s) 591
NspI RCATGY 1 cut(s) 97
PaeI GCATGC 1 cut(s) 97
PceI AGGCCT 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 346
PctI GAATGC 2 cut(s) 8, 61
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 469, 817
PfoI TCCNGGA 1 cut(s) 172
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 218
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 671
RruI TCGCGA 1 cut(s) 591
RsaI GTAC 1 cut(s) 664
RsaNI GTAC 1 cut(s) 663
Sau3AI GATC 3 cut(s) 112, 337, 592
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 3 cut(s) 115, 384, 582
SfcI CTRYAG 1 cut(s) 672
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SphI GCATGC 1 cut(s) 97
Sse9I AATT 6 cut(s) 116, 382, 582, 621, 780, 825
SseBI AGGCCT 1 cut(s) 199
SsiI CCGC 1 cut(s) 280
SspMI CTAG 1 cut(s) 449
StuI AGGCCT 1 cut(s) 199
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 2 cut(s) 242, 324
TaqI TCGA 3 cut(s) 123, 324, 565
TasI AATT 6 cut(s) 116, 382, 582, 621, 780, 825
TatI WGTACW 1 cut(s) 662
TfiI GAWTC 2 cut(s) 469, 817
TspDTI ATGAA 6 cut(s) 17, 33, 83, 490, 648, 666
VpaK11BI GGWCC 1 cut(s) 218
XagI CCTNNNNNAGG 1 cut(s) 225
XapI RAATTY 1 cut(s) 780
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.