FvH4_2g32430

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008582
Physical Location & Seq
Forward (+)
24586765 .. 24589903
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32430.t1

Sequence Viewer

Length: 1182 bp
ATGATTCGAAGGACTGCATGTTTGAGTAAGCTGGTTCGGTCTTACAAAGTTGGCTCTTCGTCATCTTTGATAACCAAGCAGCTTGATCATCGTTGCTTTGTATCAGCTTCTGCTTCTAGCGGAGAACAAGCGTGTCAAGCATATGTATATAGTGAATTTGGTAGGAACGAGTATGCTGATGTGGATTGGGATAATCTTGGATTTGGTCTAGTGCCAACAGATTACATGTACATCATGAAAGGTTCCAGCAACGGGATATTTGAACAAGGCCAACTTAATCGTTATGGAAATATTGAACTTAACCCTGCTGCTGGAGTTTTGAATTATGGACAGGGTTTATATGAAGGGATGAAAGCATTCAGGAAAGAAAATGGAAATCTCCTCCTCTTTCGTCCAGACCAGAATGCAATGCGAATGAAGATTGGTTCAGAAAGAATGTGCATGCCTTCGCCATCCCTTGATCAATTTATTGATGCTGTGAAGCAAACTGCTCAAGCTAACAAGTGCTGGGTTCCTCCTCCAGGAAAAGGGTCTTTGTATATTAGGCCTCTACTTATAGGAAGTGGTCCTATATTAGGTTTAGCCCCATCGCCTGAATATACATTCCTTGTATACGCTTCACCAGTCCGCAACTATTTCAAGGAGGGTTCTGCACCTTTGAGCATATACGTCGAGGAAGAATATGATCGTGCCTCTCGTGGTGGGGCTGGAGGAGTCAAAAGCATCACCAATTATGCGCCAGTTTTGAAGGCACTGCTCAGAGCAAAGAGCCAAGGATTTTCTGATGTGTTATATCTGGACTCGGTAAATAGAAAGAATCTGGAGGAGGTCTCTTCTTGCAACATTTTCATTGTCAAGGGCAATGTTATTTCAACTCCTGCAACGGCCGGGACTATACTATCAGGGATAACTCGAAGAAGCATCATTGAAATCGCTCGTGATCTTGGTTACCAGGTTGAGGAGCGTGCAATTCCGGTGGATGAATTGAGTGAAGCTGATGAAGTATTCTGCACAGGAACCGCAGTTGGTGTTGCTCCTGTGGGAAACATTACATATAAAGATAAAAGGATGGAATACAGAACAGGTGCCCAAACTGTTTGTCCACAGCTCTACTCGACTATCATAGGAATCCAAACGGGTCGTATTGAGGATAAGAAAGGCTGGATTCTCGAAATTGATTGA

Protein Analysis

394

Amino Acids

43.16

Weight (kDa)

7.99

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 112 - 350 1.1e-37 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1085
AccI GTMKAC 1 cut(s) 612
AciI CCGC 3 cut(s) 120, 628, 1020
AcoI YGGCCR 1 cut(s) 885
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 6 cut(s) 252, 311, 521, 527, 575, 958
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 7 cut(s) 263, 296, 322, 640, 748, 873, 929
AjnI CCWGG 2 cut(s) 520, 951
AluBI AGCT 6 cut(s) 31, 82, 107, 497, 995, 1108
AluI AGCT 6 cut(s) 31, 82, 107, 497, 995, 1108
Alw26I GTCTC 1 cut(s) 835
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 3 cut(s) 268, 545, 885
ApeKI GCWGC 2 cut(s) 79, 308
ApoI RAATTY 1 cut(s) 155
ArsI GACNNNNNNTTYG 3 cut(s) 36, 1084, 1116
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 1 cut(s) 889
AsuHPI GGTGA 2 cut(s) 612, 718
AsuII TTCGAA 1 cut(s) 7
AvaII GGWCC 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 1085
BauI CACGAG 2 cut(s) 696, 936
BbvI GCAGC 2 cut(s) 91, 295
BccI CCATC 3 cut(s) 460, 595, 1063
BceAI ACGGC 1 cut(s) 900
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 2 cut(s) 522, 953
BclI TGATCA 2 cut(s) 85, 460
BcnI CCSGG 1 cut(s) 889
BcoDI GTCTC 1 cut(s) 835
BfaI CTAG 2 cut(s) 117, 209
BisI GCNGC 2 cut(s) 80, 309
BlsI GCNGC 2 cut(s) 81, 310
Bme1390I CCNGG 3 cut(s) 522, 889, 953
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 4 cut(s) 244, 513, 1018, 1087
BmrFI CCNGG 3 cut(s) 522, 889, 953
BmsI GCATC 3 cut(s) 463, 732, 930
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 4 cut(s) 333, 504, 729, 842
Bpu14I TTCGAA 1 cut(s) 7
BpuEI CTTGAG 1 cut(s) 477
BpuMI CCSGG 1 cut(s) 889
BsaI GGTCTC 1 cut(s) 835
BsaJI CCNNGG 1 cut(s) 772
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 6 cut(s) 252, 311, 521, 527, 575, 958
Bse1I ACTGG 2 cut(s) 623, 740
Bse3DI GCAATG 2 cut(s) 414, 868
BseBI CCWGG 2 cut(s) 522, 953
BseDI CCNNGG 1 cut(s) 772
BseGI GGATG 4 cut(s) 354, 452, 985, 1074
BseLI CCNNNNNNNGG 6 cut(s) 252, 311, 521, 527, 575, 958
BseMI GCAATG 2 cut(s) 414, 868
BseMII CTCAG 1 cut(s) 772
BseNI ACTGG 2 cut(s) 623, 740
BseRI GAGGAG 6 cut(s) 371, 374, 507, 726, 839, 974
BseSI GKGCMC 1 cut(s) 1090
BseX3I CGGCCG 1 cut(s) 885
BseXI GCAGC 2 cut(s) 91, 295
BseYI CCCAGC 1 cut(s) 507
BsgI GTGCAG 2 cut(s) 636, 994
Bsh1285I CGRYCG 1 cut(s) 888
BshFI GGCC 3 cut(s) 270, 547, 887
BshNI GGYRCC 1 cut(s) 1085
BsiEI CGRYCG 1 cut(s) 888
BsiSI CCGG 2 cut(s) 888, 974
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 6 cut(s) 252, 311, 521, 527, 575, 958
BsmAI GTCTC 1 cut(s) 835
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 2 cut(s) 356, 409
BsnI GGCC 3 cut(s) 270, 547, 887
Bso31I GGTCTC 1 cut(s) 835
Bsp119I TTCGAA 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 4 cut(s) 85, 460, 685, 940
BspACI CCGC 3 cut(s) 120, 628, 1020
BspANI GGCC 3 cut(s) 270, 547, 887
BspCNI CTCAG 1 cut(s) 771
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 4 cut(s) 244, 513, 1018, 1087
BspQI GCTCTTC 1 cut(s) 61
BspT104I TTCGAA 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 1085
BspTNI GGTCTC 1 cut(s) 835
BsrDI GCAATG 2 cut(s) 414, 868
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 2 cut(s) 623, 740
BssECI CCNNGG 1 cut(s) 772
BssMI GATC 4 cut(s) 85, 460, 685, 940
BssNAI GTATAC 1 cut(s) 613
BssSI CACGAG 2 cut(s) 696, 936
BssT1I CCWWGG 1 cut(s) 772
Bst1107I GTATAC 1 cut(s) 613
Bst2BI CACGAG 2 cut(s) 696, 936
Bst2UI CCWGG 2 cut(s) 522, 953
Bst4CI ACNGT 1 cut(s) 1096
Bst6I CTCTTC 2 cut(s) 61, 838
BstAUI TGTACA 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 2 cut(s) 443, 966
BstDEI CTNAG 1 cut(s) 758
BstEII GGTNACC 1 cut(s) 947
BstENI CCTNNNNNAGG 2 cut(s) 519, 573
BstF5I GGATG 4 cut(s) 354, 452, 985, 1074
BstHHI GCGC 1 cut(s) 739
BstKTI GATC 4 cut(s) 88, 463, 688, 943
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 4 cut(s) 85, 460, 685, 940
BstMCI CGRYCG 1 cut(s) 888
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 2 cut(s) 522, 953
BstNSI RCATGY 3 cut(s) 21, 229, 445
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 520, 887, 951
BstSLI GKGCMC 1 cut(s) 1090
BstV1I GCAGC 2 cut(s) 91, 295
BstZ17I GTATAC 1 cut(s) 613
BstZI CGGCCG 1 cut(s) 885
BsuRI GGCC 3 cut(s) 270, 547, 887
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 4 cut(s) 354, 452, 985, 1074
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 1 cut(s) 752
Cac8I GCNNGC 2 cut(s) 443, 966
CaiI CAGNNNCTG 1 cut(s) 110
CciI TCATGA 1 cut(s) 234
CfoI GCGC 1 cut(s) 739
Cfr13I GGNCC 1 cut(s) 566
CsiI ACCWGGT 1 cut(s) 951
Csp6I GTAC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 957, 992
CviAII CATG 4 cut(s) 18, 226, 235, 442
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 758
DpnI GATC 4 cut(s) 87, 462, 687, 942
DpnII GATC 4 cut(s) 85, 460, 685, 940
EaeI YGGCCR 1 cut(s) 885
EagI CGGCCG 1 cut(s) 885
Eam1104I CTCTTC 2 cut(s) 61, 838
EarI CTCTTC 2 cut(s) 61, 838
EclXI CGGCCG 1 cut(s) 885
Eco130I CCWWGG 1 cut(s) 772
Eco147I AGGCCT 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 566
Eco52I CGGCCG 1 cut(s) 885
Eco91I GGTNACC 1 cut(s) 947
EcoNI CCTNNNNNAGG 2 cut(s) 519, 573
EcoO65I GGTNACC 1 cut(s) 947
EcoRII CCWGG 2 cut(s) 520, 951
EcoT14I CCWWGG 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 4 cut(s) 21, 229, 238, 445
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 904
FatI CATG 4 cut(s) 17, 225, 234, 441
FauNDI CATATG 1 cut(s) 142
FbaI TGATCA 2 cut(s) 85, 460
FblI GTMKAC 1 cut(s) 612
Fnu4HI GCNGC 2 cut(s) 80, 309
FokI GGATG 4 cut(s) 361, 439, 992, 1081
Fsp4HI GCNGC 2 cut(s) 80, 309
FspBI CTAG 2 cut(s) 117, 209
GlaI GCGC 1 cut(s) 738
GluI GCNGC 2 cut(s) 80, 309
GsaI CCCAGC 1 cut(s) 511
GsuI CTGGAG 4 cut(s) 333, 504, 729, 842
HaeIII GGCC 3 cut(s) 270, 547, 887
HapII CCGG 2 cut(s) 888, 974
HhaI GCGC 1 cut(s) 739
Hin1II CATG 4 cut(s) 21, 229, 238, 445
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HinfI GANTC 6 cut(s) 4, 714, 800, 817, 1128, 1165
HpaII CCGG 2 cut(s) 888, 974
HphI GGTGA 2 cut(s) 612, 718
Hpy166II GTNNAC 2 cut(s) 613, 1103
Hpy188I TCNGA 3 cut(s) 430, 761, 784
Hpy188III TCNNGA 7 cut(s) 235, 361, 395, 797, 821, 938, 1169
Hpy8I GTNNAC 2 cut(s) 613, 1103
Hpy99I CGWCG 1 cut(s) 674
HpyAV CCTTC 4 cut(s) 3, 338, 456, 742
HpyCH4III ACNGT 1 cut(s) 1096
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 8 cut(s) 17, 407, 441, 653, 840, 881, 968, 1011
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 1 cut(s) 758
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 4 cut(s) 21, 229, 238, 445
HspAI GCGC 1 cut(s) 737
Ksp22I TGATCA 2 cut(s) 85, 460
Kzo9I GATC 4 cut(s) 85, 460, 685, 940
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 2 cut(s) 961, 1039
Lsp1109I GCAGC 2 cut(s) 91, 295
LweI GCATC 3 cut(s) 463, 732, 930
MabI ACCWGGT 1 cut(s) 951
MaeI CTAG 2 cut(s) 117, 209
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 1 cut(s) 947
MalI GATC 4 cut(s) 87, 462, 687, 942
MboI GATC 4 cut(s) 85, 460, 685, 940
MboII GAAGA 5 cut(s) 48, 430, 689, 825, 927
MhlI GDGCHC 1 cut(s) 1090
MluCI AATT 7 cut(s) 155, 322, 464, 730, 969, 983, 1173
MlyI GAGTC 2 cut(s) 723, 794
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 2 cut(s) 276, 300
MspI CCGG 2 cut(s) 888, 974
MspR9I CCNGG 3 cut(s) 522, 889, 953
Mva1269I GAATGC 2 cut(s) 356, 409
MvaI CCWGG 2 cut(s) 522, 953
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 889
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 85, 460, 685, 940
NlaIII CATG 4 cut(s) 21, 229, 238, 445
NlaIV GGNNCC 4 cut(s) 244, 513, 1018, 1087
NspI RCATGY 3 cut(s) 21, 229, 445
NspV TTCGAA 1 cut(s) 7
PaeI GCATGC 1 cut(s) 445
PagI TCATGA 1 cut(s) 234
PceI AGGCCT 1 cut(s) 547
PciI ACATGT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 694
PctI GAATGC 2 cut(s) 356, 409
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 4 cut(s) 4, 817, 1128, 1165
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 2 cut(s) 81, 310
PleI GAGTC 2 cut(s) 722, 794
PpsI GAGTC 2 cut(s) 722, 794
PscI ACATGT 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 520, 951
PspEI GGTNACC 1 cut(s) 947
PspFI CCCAGC 1 cut(s) 507
PspGI CCWGG 2 cut(s) 520, 951
PspN4I GGNNCC 4 cut(s) 244, 513, 1018, 1087
PspPI GGNCC 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 276, 300
SatI GCNGC 2 cut(s) 80, 309
Sau3AI GATC 4 cut(s) 85, 460, 685, 940
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 2 cut(s) 723, 794
ScrFI CCNGG 3 cut(s) 522, 889, 953
SduI GDGCHC 1 cut(s) 1090
SexAI ACCWGGT 1 cut(s) 951
SfaNI GCATC 3 cut(s) 463, 732, 930
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 492
SmoI CTYRAG 1 cut(s) 492
SphI GCATGC 1 cut(s) 445
Sse9I AATT 7 cut(s) 155, 322, 464, 730, 969, 983, 1173
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 3 cut(s) 120, 628, 1020
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 117, 209
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 3 cut(s) 520, 887, 951
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 1 cut(s) 1096
TaiI ACGT 1 cut(s) 672
TaqI TCGA 5 cut(s) 7, 672, 913, 1115, 1170
TaqII GACCGA 1 cut(s) 27
TasI AATT 7 cut(s) 155, 322, 464, 730, 969, 983, 1173
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 4 cut(s) 4, 817, 1128, 1165
Tru1I TTAA 2 cut(s) 276, 300
Tru9I TTAA 2 cut(s) 276, 300
TscAI CASTG 1 cut(s) 759
TseI GCWGC 2 cut(s) 79, 308
TspDTI ATGAA 7 cut(s) 251, 357, 365, 431, 838, 996, 1014
TspRI CASTG 1 cut(s) 759
VpaK11BI GGWCC 1 cut(s) 566
XagI CCTNNNNNAGG 2 cut(s) 519, 573
XapI RAATTY 1 cut(s) 155
XceI RCATGY 3 cut(s) 21, 229, 445
XmiI GTMKAC 1 cut(s) 612
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 2 cut(s) 117, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.