Rh6DG493400

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008582
Physical Location & Seq
Reverse (-)
65251850 .. 65254524
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG493400.1

Sequence Viewer

Length: 1182 bp
ATGATTCGAAGGACTGCATGCTTGAGTAAGCTGGTTCAATCTGTTCGAGTTGGCTCTTCGTCATCTTTGCCAACCAAGCTTAGAGATCACCGCTGCTTTGGATCAGGGGCTGCTTCTAACGCAGAACAAGCGTGTGAAGCATCTGTGTGCAGTAAATTTGGTAAGAATGAGTATGCTGATGTGGATTGGGATAATCTTGGATTTGGTCTAGTGCCAACTGATTACATGTACATGATGAAATGTTCCAACAACGGGATATTCGAACAAGGCCAACTTAATCGTTATGGAAATATTGAACTAAGCCCTGCTGCTGGAGTTTTGAATTATGGCCAGGGTATATATGAAGGGATGAAAGCATTCAGGAAAGAAGATGGCAATCTCCTTATCTTTCGTCCAGACCAGAATGCAATGCGAATGAAGACTGGTTCAGAAAGAATGTGCATGCCCTCACCATCCCTTGATCAATTTATCGATGCTGTGAAGCAAACTGCTCAAGCTAACAAGTGTTGGGTTCCCCCTCCAGGAAAAGGGTCTTTATATATTAGGCCTCTACTTATAGGAAGTGGTCCTATATTAGGTTTAAGCACATCGCCTGAATACACATTCCTTGTATATGCTTCACCAGTCCGCAACTATTTCAAGGAGGGTTCTGCACCTTTGAGCATATACGTCGAGGAGGAATATGATCGTTCCTCTCGTGGTGGGGCTGGAGGTGTCAAAAGCATCACCAATTATGCTCCAGTTTTGAAGGCACTACTCAGAGCAAGGAGCCGAGGATTTTCTGATGTGTTATATCTAGACTCGGTAAATAAAAAGAATCTGGAGGAGGTCTCTTCTTGTAACATTTTCATTGTGAAGGGCAATGTTATTTCAACTCCTGCAACGGCTGGGACTATACTATCAGGGGTAACTCGAAGAAGCATCATTGAAATCGCTTGCGATCATGGTTATCAGGTTGAGGAGCGTGCAATTCCGGTAGATGAACTGAGTGAAGCTGATGAAGTATTCTGTACAGGAACTGCAGTCGGTGTTGCTCCTGTGGGAAACATTACATATAAAGATAAAAGGATGGAATACAAAACAGGTGCTCAAACTGTTTGTCCACAGCTCTACTCAACTCTCATAGGAATTCAAACGGGTCGTATTGAGGATAAGAAAAGCTGGATTCTTGAAATTGATTAA

Protein Analysis

393

Amino Acids

42.99

Weight (kDa)

7.91

Isoelectric Point (pI)

57.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 112 - 350 2.6e-36 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 91, 628
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 155, 1128
AfaI GTAC 2 cut(s) 230, 1012
AfiI CCNNNNNNNGG 5 cut(s) 252, 311, 521, 527, 575
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 9 cut(s) 38, 296, 322, 640, 748, 873, 929, 1133, 1172
AjnI CCWGG 2 cut(s) 330, 520
AluBI AGCT 6 cut(s) 31, 79, 497, 995, 1108, 1161
AluI AGCT 6 cut(s) 31, 79, 497, 995, 1108, 1161
Alw21I GWGCWC 1 cut(s) 1090
Alw26I GTCTC 1 cut(s) 835
AlwI GGATC 1 cut(s) 109
AlwNI CAGNNNCTG 2 cut(s) 110, 1019
AoxI GGCC 3 cut(s) 268, 328, 545
ApeKI GCWGC 3 cut(s) 93, 110, 308
ApoI RAATTY 2 cut(s) 155, 1128
ArsI GACNNNNNNTTYG 2 cut(s) 1084, 1116
Asp700I GAANNNNTTC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 566
AsuHPI GGTGA 4 cut(s) 80, 441, 612, 718
AsuII TTCGAA 2 cut(s) 7, 261
AvaII GGWCC 1 cut(s) 566
BalI TGGCCA 1 cut(s) 330
BauI CACGAG 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 1090
BbvI GCAGC 3 cut(s) 80, 97, 295
BccI CCATC 3 cut(s) 365, 460, 1063
BceAI ACGGC 1 cut(s) 900
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 2 cut(s) 332, 522
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 835
BfaI CTAG 2 cut(s) 209, 797
BfmI CTRYAG 1 cut(s) 1020
BisI GCNGC 3 cut(s) 94, 111, 309
BlsI GCNGC 3 cut(s) 95, 112, 310
Bme1390I CCNGG 2 cut(s) 332, 522
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 513, 770
BmrFI CCNGG 2 cut(s) 332, 522
BmsI GCATC 4 cut(s) 149, 463, 732, 930
BpiI GAAGAC 1 cut(s) 425
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 5 cut(s) 333, 504, 723, 729, 842
Bpu14I TTCGAA 2 cut(s) 7, 261
BpuEI CTTGAG 2 cut(s) 43, 477
Bsa29I ATCGAT 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 835
BsaJI CCNNGG 2 cut(s) 331, 772
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 5 cut(s) 252, 311, 521, 527, 575
Bse1I ACTGG 3 cut(s) 427, 623, 740
Bse3DI GCAATG 2 cut(s) 414, 868
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 2 cut(s) 332, 522
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 331, 772
BseGI GGATG 3 cut(s) 354, 452, 1074
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 5 cut(s) 252, 311, 521, 527, 575
BseMI GCAATG 2 cut(s) 414, 868
BseMII CTCAG 2 cut(s) 772, 977
BseNI ACTGG 3 cut(s) 427, 623, 740
BseRI GAGGAG 3 cut(s) 689, 839, 974
BseXI GCAGC 3 cut(s) 80, 97, 295
BseYI CCCAGC 1 cut(s) 887
BsgI GTGCAG 2 cut(s) 169, 636
BshFI GGCC 3 cut(s) 270, 330, 547
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 1090
BsiSI CCGG 1 cut(s) 974
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 5 cut(s) 252, 311, 521, 527, 575
BsmAI GTCTC 1 cut(s) 835
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 2 cut(s) 356, 409
BsnI GGCC 3 cut(s) 270, 330, 547
Bso31I GGTCTC 1 cut(s) 835
Bsp119I TTCGAA 2 cut(s) 7, 261
Bsp1286I GDGCHC 1 cut(s) 1090
Bsp1407I TGTACA 2 cut(s) 228, 1010
Bsp143I GATC 5 cut(s) 85, 101, 460, 685, 940
BspACI CCGC 2 cut(s) 91, 628
BspANI GGCC 3 cut(s) 270, 330, 547
BspCNI CTCAG 2 cut(s) 771, 978
BspDI ATCGAT 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 513, 770
BspMAI CTGCAG 1 cut(s) 1024
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 61
BspT104I TTCGAA 2 cut(s) 7, 261
BspTNI GGTCTC 1 cut(s) 835
BsrDI GCAATG 2 cut(s) 414, 868
BsrGI TGTACA 2 cut(s) 228, 1010
BsrI ACTGG 3 cut(s) 427, 623, 740
BssECI CCNNGG 2 cut(s) 331, 772
BssMI GATC 5 cut(s) 85, 101, 460, 685, 940
BssSI CACGAG 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 332, 522
Bst4CI ACNGT 1 cut(s) 1096
Bst6I CTCTTC 2 cut(s) 61, 838
BstAUI TGTACA 2 cut(s) 228, 1010
BstBI TTCGAA 2 cut(s) 7, 261
BstC8I GCNNGC 4 cut(s) 19, 443, 937, 966
BstDEI CTNAG 4 cut(s) 80, 299, 758, 986
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 3 cut(s) 354, 452, 1074
BstKTI GATC 5 cut(s) 88, 104, 463, 688, 943
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 5 cut(s) 85, 101, 460, 685, 940
BstMWI GCNNNNNNNGC 4 cut(s) 76, 119, 128, 137
BstNI CCWGG 2 cut(s) 332, 522
BstNSI RCATGY 3 cut(s) 21, 229, 445
BstSCI CCNGG 2 cut(s) 330, 520
BstSFI CTRYAG 1 cut(s) 1020
BstV1I GCAGC 3 cut(s) 80, 97, 295
BstV2I GAAGAC 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 3 cut(s) 270, 330, 547
BsuTUI ATCGAT 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 3 cut(s) 354, 452, 1074
Cac8I GCNNGC 4 cut(s) 19, 443, 937, 966
CaiI CAGNNNCTG 2 cut(s) 110, 1019
Cfr13I GGNCC 1 cut(s) 566
ClaI ATCGAT 1 cut(s) 471
Csp6I GTAC 2 cut(s) 229, 1011
CviAII CATG 5 cut(s) 18, 226, 232, 442, 944
CviQI GTAC 2 cut(s) 229, 1011
DdeI CTNAG 4 cut(s) 80, 299, 758, 986
DpnI GATC 5 cut(s) 87, 103, 462, 687, 942
DpnII GATC 5 cut(s) 85, 101, 460, 685, 940
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 61, 838
EarI CTCTTC 2 cut(s) 61, 838
Eco147I AGGCCT 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 566
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 1128
EcoRII CCWGG 2 cut(s) 330, 520
FaeI CATG 5 cut(s) 21, 229, 235, 445, 947
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 904
FatI CATG 5 cut(s) 17, 225, 231, 441, 943
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 94, 111, 309
FokI GGATG 3 cut(s) 361, 439, 1081
Fsp4HI GCNGC 3 cut(s) 94, 111, 309
FspBI CTAG 2 cut(s) 209, 797
GluI GCNGC 3 cut(s) 94, 111, 309
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 5 cut(s) 333, 504, 723, 729, 842
HaeIII GGCC 3 cut(s) 270, 330, 547
HapII CCGG 1 cut(s) 974
Hin1II CATG 5 cut(s) 21, 229, 235, 445, 947
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 4 cut(s) 4, 800, 817, 1165
HpaII CCGG 1 cut(s) 974
HphI GGTGA 4 cut(s) 80, 441, 612, 718
Hpy166II GTNNAC 1 cut(s) 1103
Hpy188I TCNGA 3 cut(s) 430, 761, 784
Hpy188III TCNNGA 5 cut(s) 361, 395, 797, 821, 1169
Hpy8I GTNNAC 1 cut(s) 1103
Hpy99I CGWCG 1 cut(s) 674
HpyAV CCTTC 4 cut(s) 3, 338, 742, 850
HpyCH4III ACNGT 1 cut(s) 1096
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 8 cut(s) 17, 150, 407, 441, 653, 881, 968, 1022
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 119, 128, 137
HpyF3I CTNAG 4 cut(s) 80, 299, 758, 986
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 5 cut(s) 21, 229, 235, 445, 947
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 5 cut(s) 85, 101, 460, 685, 940
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 4 cut(s) 742, 768, 961, 1039
Lsp1109I GCAGC 3 cut(s) 80, 97, 295
LweI GCATC 4 cut(s) 149, 463, 732, 930
MaeI CTAG 2 cut(s) 209, 797
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 839, 907
MalI GATC 5 cut(s) 87, 103, 462, 687, 942
MboI GATC 5 cut(s) 85, 101, 460, 685, 940
MboII GAAGA 5 cut(s) 48, 380, 430, 825, 927
MhlI GDGCHC 1 cut(s) 1090
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 7 cut(s) 155, 322, 464, 730, 969, 1128, 1173
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 1 cut(s) 270
Mox20I TGGCCA 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 356
MscI TGGCCA 1 cut(s) 330
MseI TTAA 3 cut(s) 276, 581, 1180
MslI CAYNNNNRTG 2 cut(s) 145, 230
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 1 cut(s) 974
MspR9I CCNGG 2 cut(s) 332, 522
Mva1269I GAATGC 2 cut(s) 356, 409
MvaI CCWGG 2 cut(s) 332, 522
MwoI GCNNNNNNNGC 4 cut(s) 76, 119, 128, 137
NdeII GATC 5 cut(s) 85, 101, 460, 685, 940
NlaIII CATG 5 cut(s) 21, 229, 235, 445, 947
NlaIV GGNNCC 2 cut(s) 513, 770
NmeAIII GCCGAG 1 cut(s) 797
NspI RCATGY 3 cut(s) 21, 229, 445
NspV TTCGAA 2 cut(s) 7, 261
PaeI GCATGC 2 cut(s) 21, 445
PceI AGGCCT 1 cut(s) 547
PciI ACATGT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 694
PctI GAATGC 2 cut(s) 356, 409
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 3 cut(s) 4, 817, 1165
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 3 cut(s) 95, 112, 310
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
PscI ACATGT 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 330, 520
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 2 cut(s) 330, 520
PspN4I GGNNCC 2 cut(s) 513, 770
PspPI GGNCC 1 cut(s) 566
PstI CTGCAG 1 cut(s) 1024
PstNI CAGNNNCTG 2 cut(s) 110, 1019
RsaI GTAC 2 cut(s) 230, 1012
RsaNI GTAC 2 cut(s) 229, 1011
RseI CAYNNNNRTG 2 cut(s) 145, 230
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 3 cut(s) 276, 581, 1180
SatI GCNGC 3 cut(s) 94, 111, 309
Sau3AI GATC 5 cut(s) 85, 101, 460, 685, 940
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 2 cut(s) 332, 522
SduI GDGCHC 1 cut(s) 1090
SfaNI GCATC 4 cut(s) 149, 463, 732, 930
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 2 cut(s) 7, 261
SinI GGWCC 1 cut(s) 566
SmiMI CAYNNNNRTG 2 cut(s) 145, 230
SmlI CTYRAG 2 cut(s) 22, 492
SmoI CTYRAG 2 cut(s) 22, 492
SphI GCATGC 2 cut(s) 21, 445
Sse9I AATT 7 cut(s) 155, 322, 464, 730, 969, 1128, 1173
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 2 cut(s) 91, 628
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 209, 797
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 2 cut(s) 330, 520
TaaI ACNGT 1 cut(s) 1096
TaiI ACGT 1 cut(s) 672
TaqI TCGA 6 cut(s) 7, 46, 261, 471, 672, 913
TasI AATT 7 cut(s) 155, 322, 464, 730, 969, 1128, 1173
TatI WGTACW 2 cut(s) 228, 1010
TfiI GAWTC 3 cut(s) 4, 817, 1165
Tru1I TTAA 3 cut(s) 276, 581, 1180
Tru9I TTAA 3 cut(s) 276, 581, 1180
TseI GCWGC 3 cut(s) 93, 110, 308
TspDTI ATGAA 7 cut(s) 251, 357, 365, 431, 838, 996, 1014
VpaK11BI GGWCC 1 cut(s) 566
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 2 cut(s) 155, 1128
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 3 cut(s) 21, 229, 445
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 2 cut(s) 209, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.