AT1G10280

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012465
Physical Location & Seq
Reverse (-)
3366493 .. 3369125
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10280.1

Sequence Viewer

Length: 1239 bp
ATGGCGAGAGGAGGGAAAGAGGAAGGAGAAAAACACATAGGGTTACTAAAGCTGGCGCAAACGCTGTCGTTTTTGCTAATTTTCATGGCTGGAATCATCATCGGTCTCGCCGCAAGTTCTCACATCGATCGCTACTTCAATTCTTTGCCGAGGATGTTCTCATCAACCACTAACCTCCAATCGATTCCTTTTTCGACTCCTGATTACTCCAATTGCACAATCATCCACCGAGACTGCACAGGTAACGACGACAACGAGAGCGACGACGGAGGAGTCAAGGCGGAGAAACCAAAGGTTCGAGATTGTTGGAGCATTGATGGGTTTGTTCGGCCAGAGAATCTGAGTCATGGAATGACTGATGATGAGTTGTTCTGGAGAGCTTCGATGGTTCCGGTGAAGGAAGAGTATCCGTACGATAGGGTTCCGAAAGTGGCGTTTATGTTTCTGACGAGAGGGCCTTTACCTATGCTTCCGCTTTGGGAGAAGTTTTTTAAAGGCAATGAAAAGTACTTGTCCGTTTATGTTCATACGCCTCCTGGATACGACATGAATGTCTCTCGTGATTCTCCCTTTTACGACCGCCAGATCCCCAGCCAGAGAGTTGAATGGGGATCCCCACTATTGACAGACGCAGAGAAGCGTCTTCTAGCCAATGCATTGCTGGACTTCTCAAATGAGCGTTTTGTTCTTCTCTCAGAGAGCTGTGTCCCAGTTTACAACTTCTCAACTGTCTACACTTACCTAATAAACTCTGCTTACAGTTTTGTGGACTCTTATGATGAGCCAACACGTTATGGCCGTGGCCGTTATAGCCGCAAGATGCTCCCGGATATCAAACTCCATCACTGGCGCAAAGGGTCTCAGTGGTTTGAAGTAAACCGTAAAATCGCCATCTACATCATCTCAGACTCGAAATACTACTCATTGTTCAAACAATTCTGCAGACCAGCTTGTTACCCAGACGAGCATTACATCCCTACTTTCTTGAACATGTTTCACGGTTCAATGAACGCAAATAGAAGCGTGACATGGGTTGATTGGTCCATAGGCGGTCCCCATCCAGCTACATATGCTGCAGCTAATATCACAGAAGGGTTTCTCCAATCTATAAGGAAAAACGAGACGGATTGTCTTTACAATGAAGAGCCAACTTCGTTATGCTTCCTTTTTGCTCGCAAGTTCTCTCCTTCTGCCTTAGCTCCTCTTATGAATTTGAGCTCTACTGTCCTGGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

412

Amino Acids

47.25

Weight (kDa)

6.56

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 144 - 371 1e-83 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 272, 551
AccI GTMKAC 1 cut(s) 732
AciI CCGC 6 cut(s) 111, 281, 473, 580, 814, 1050
AclWI GGATC 3 cut(s) 580, 606, 619
AcoI YGGCCR 3 cut(s) 329, 796, 802
AcsI RAATTY 1 cut(s) 1210
AfaI GTAC 2 cut(s) 413, 509
AflIII ACRYGT 2 cut(s) 788, 990
AgsI TTSAA 6 cut(s) 139, 605, 872, 931, 988, 1005
AhdI GACNNNNNGTC 1 cut(s) 1128
AjnI CCWGG 2 cut(s) 535, 1227
AluBI AGCT 8 cut(s) 52, 380, 702, 950, 1064, 1079, 1199, 1218
AluI AGCT 8 cut(s) 52, 380, 702, 950, 1064, 1079, 1199, 1218
Alw21I GWGCWC 1 cut(s) 1220
Alw26I GTCTC 5 cut(s) 110, 225, 559, 864, 1115
AlwI GGATC 3 cut(s) 580, 606, 619
AoxI GGCC 4 cut(s) 329, 455, 796, 802
ApeKI GCWGC 2 cut(s) 1073, 1076
ApoI RAATTY 1 cut(s) 1210
Asp700I GAANNNNTTC 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 58, 852
AspS9I GGNCC 3 cut(s) 455, 1041, 1052
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 1 cut(s) 406
AvaII GGWCC 2 cut(s) 1041, 1052
BamHI GGATCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 1220
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 1 cut(s) 1220
BbvI GCAGC 2 cut(s) 1060, 1088
BccI CCATC 5 cut(s) 311, 379, 849, 899, 1065
BceAI ACGGC 2 cut(s) 783, 789
BciT130I CCWGG 2 cut(s) 537, 1229
BciVI GTATCC 2 cut(s) 417, 533
BcnI CCSGG 1 cut(s) 827
BcoDI GTCTC 5 cut(s) 110, 225, 559, 864, 1115
BfaI CTAG 1 cut(s) 647
BfmI CTRYAG 2 cut(s) 940, 1074
BfuI GTATCC 2 cut(s) 417, 533
BisI GCNGC 4 cut(s) 111, 814, 1074, 1077
BlsI GCNGC 4 cut(s) 112, 815, 1075, 1078
BmcAI AGTACT 1 cut(s) 509
Bme1390I CCNGG 3 cut(s) 537, 827, 1229
Bme18I GGWCC 2 cut(s) 1041, 1052
BmeRI GACNNNNNGTC 1 cut(s) 1128
BmgT120I GGNCC 3 cut(s) 455, 1041, 1052
BmiI GGNNCC 4 cut(s) 390, 423, 613, 1054
BmrFI CCNGG 3 cut(s) 537, 827, 1229
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 1 cut(s) 810
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 1195
BpuMI CCSGG 1 cut(s) 827
Bsa29I ATCGAT 2 cut(s) 126, 182
BsaI GGTCTC 2 cut(s) 110, 864
BsaJI CCNNGG 3 cut(s) 149, 799, 1228
BsaWI WCCGGW 1 cut(s) 391
Bse1I ACTGG 2 cut(s) 710, 851
Bse3DI GCAATG 2 cut(s) 505, 656
BseBI CCWGG 2 cut(s) 537, 1229
BseCI ATCGAT 2 cut(s) 126, 182
BseDI CCNNGG 3 cut(s) 149, 799, 1228
BseGI GGATG 4 cut(s) 159, 222, 972, 1057
BseMI GCAATG 2 cut(s) 505, 656
BseMII CTCAG 4 cut(s) 332, 708, 875, 918
BseNI ACTGG 2 cut(s) 710, 851
BseRI GAGGAG 3 cut(s) 24, 285, 1191
BseXI GCAGC 2 cut(s) 1060, 1088
BseYI CCCAGC 1 cut(s) 590
BsgI GTGCAG 1 cut(s) 220
Bsh1285I CGRYCG 2 cut(s) 130, 580
BshFI GGCC 4 cut(s) 331, 457, 798, 804
BshVI ATCGAT 2 cut(s) 126, 182
BsiEI CGRYCG 2 cut(s) 130, 580
BsiHKAI GWGCWC 1 cut(s) 1220
BsiSI CCGG 2 cut(s) 392, 827
BsiWI CGTACG 1 cut(s) 411
BslFI GGGAC 2 cut(s) 692, 1038
BsmAI GTCTC 5 cut(s) 110, 225, 559, 864, 1115
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 2 cut(s) 692, 1038
BsnI GGCC 4 cut(s) 331, 457, 798, 804
Bso31I GGTCTC 2 cut(s) 110, 864
Bsp1286I GDGCHC 1 cut(s) 1220
Bsp143I GATC 3 cut(s) 127, 585, 611
BspACI CCGC 6 cut(s) 111, 281, 473, 580, 814, 1050
BspANI GGCC 4 cut(s) 331, 457, 798, 804
BspCNI CTCAG 4 cut(s) 333, 707, 874, 917
BspDI ATCGAT 2 cut(s) 126, 182
BspLI GGNNCC 4 cut(s) 390, 423, 613, 1054
BspMAI CTGCAG 2 cut(s) 944, 1078
BspPI GGATC 3 cut(s) 580, 606, 619
BspQI GCTCTTC 1 cut(s) 1137
BspTNI GGTCTC 2 cut(s) 110, 864
BsrDI GCAATG 2 cut(s) 505, 656
BsrI ACTGG 2 cut(s) 710, 851
BssECI CCNNGG 3 cut(s) 149, 799, 1228
BssMI GATC 3 cut(s) 127, 585, 611
BssSI CACGAG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 2 cut(s) 537, 1229
Bst4CI ACNGT 5 cut(s) 730, 761, 881, 1001, 1225
Bst6I CTCTTC 2 cut(s) 396, 1137
BstC8I GCNNGC 2 cut(s) 54, 1174
BstDEI CTNAG 5 cut(s) 341, 694, 861, 904, 1195
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 4 cut(s) 159, 222, 972, 1057
BstHHI GCGC 2 cut(s) 58, 852
BstKTI GATC 3 cut(s) 130, 588, 614
BstMAI GTCTC 5 cut(s) 110, 225, 559, 864, 1115
BstMBI GATC 3 cut(s) 127, 585, 611
BstMCI CGRYCG 2 cut(s) 130, 580
BstMWI GCNNNNNNNGC 2 cut(s) 810, 1070
BstNI CCWGG 2 cut(s) 537, 1229
BstNSI RCATGY 1 cut(s) 994
BstSCI CCNGG 3 cut(s) 535, 825, 1227
BstSFI CTRYAG 2 cut(s) 940, 1074
BstV1I GCAGC 2 cut(s) 1060, 1088
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 2 cut(s) 585, 611
BstYI RGATCY 2 cut(s) 585, 611
Bsu15I ATCGAT 2 cut(s) 126, 182
BsuI GTATCC 2 cut(s) 417, 533
BsuRI GGCC 4 cut(s) 331, 457, 798, 804
BsuTUI ATCGAT 2 cut(s) 126, 182
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 4 cut(s) 159, 222, 972, 1057
BtsIMutI CAGTG 2 cut(s) 844, 869
Cac8I GCNNGC 2 cut(s) 54, 1174
CfoI GCGC 2 cut(s) 58, 852
Cfr13I GGNCC 3 cut(s) 455, 1041, 1052
ClaI ATCGAT 2 cut(s) 126, 182
CseI GACGC 2 cut(s) 629, 638
Csp6I GTAC 2 cut(s) 412, 508
CviAII CATG 5 cut(s) 85, 347, 547, 991, 1029
CviQI GTAC 2 cut(s) 412, 508
DdeI CTNAG 5 cut(s) 341, 694, 861, 904, 1195
DpnI GATC 3 cut(s) 129, 587, 613
DpnII GATC 3 cut(s) 127, 585, 611
DraI TTTAAA 1 cut(s) 493
DrdI GACNNNNNNGTC 2 cut(s) 272, 551
DriI GACNNNNNGTC 1 cut(s) 1128
DseDI GACNNNNNNGTC 2 cut(s) 272, 551
EaeI YGGCCR 3 cut(s) 329, 796, 802
Eam1104I CTCTTC 2 cut(s) 396, 1137
Eam1105I GACNNNNNGTC 1 cut(s) 1128
EarI CTCTTC 2 cut(s) 396, 1137
EciI GGCGGA 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 1218
Eco24I GRGCYC 1 cut(s) 1220
Eco31I GGTCTC 2 cut(s) 110, 864
Eco32I GATATC 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 1041, 1052
Eco53kI GAGCTC 1 cut(s) 1218
EcoICRI GAGCTC 1 cut(s) 1218
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 535, 1227
EcoRV GATATC 1 cut(s) 832
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 1220
Esp3I CGTCTC 1 cut(s) 1115
FaeI CATG 5 cut(s) 88, 350, 550, 994, 1032
FaqI GGGAC 2 cut(s) 692, 1038
FatI CATG 5 cut(s) 84, 346, 546, 990, 1028
FauNDI CATATG 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 4 cut(s) 111, 814, 1074, 1077
FokI GGATG 4 cut(s) 166, 209, 959, 1044
FriOI GRGCYC 1 cut(s) 1220
Fsp4HI GCNGC 4 cut(s) 111, 814, 1074, 1077
FspBI CTAG 1 cut(s) 647
GlaI GCGC 2 cut(s) 57, 851
GluI GCNGC 4 cut(s) 111, 814, 1074, 1077
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 394
HaeIII GGCC 4 cut(s) 331, 457, 798, 804
HapII CCGG 2 cut(s) 392, 827
HgaI GACGC 2 cut(s) 629, 638
HhaI GCGC 2 cut(s) 58, 852
Hin1II CATG 5 cut(s) 88, 350, 550, 994, 1032
Hin6I GCGC 2 cut(s) 56, 850
HinP1I GCGC 2 cut(s) 56, 850
HinfI GANTC 9 cut(s) 93, 184, 196, 273, 337, 343, 563, 770, 908
HpaII CCGG 2 cut(s) 392, 827
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 4 cut(s) 715, 733, 769, 877
Hpy188I TCNGA 5 cut(s) 342, 426, 447, 697, 907
Hpy188III TCNNGA 5 cut(s) 200, 299, 373, 560, 985
Hpy8I GTNNAC 4 cut(s) 715, 733, 769, 877
Hpy99I CGWCG 3 cut(s) 251, 266, 269
HpyAV CCTTC 4 cut(s) 17, 391, 1085, 1197
HpyCH4III ACNGT 5 cut(s) 730, 761, 881, 1001, 1225
HpyCH4IV ACGT 1 cut(s) 790
HpyCH4V TGCA 5 cut(s) 216, 237, 656, 942, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 810, 1070
HpyF3I CTNAG 5 cut(s) 341, 694, 861, 904, 1195
HpySE526I ACGT 1 cut(s) 790
Hsp92II CATG 5 cut(s) 88, 350, 550, 994, 1032
HspAI GCGC 2 cut(s) 56, 850
Kzo9I GATC 3 cut(s) 127, 585, 611
LguI GCTCTTC 1 cut(s) 1137
LmnI GCTCC 3 cut(s) 309, 828, 1204
Lsp1109I GCAGC 2 cut(s) 1060, 1088
LweI GCATC 1 cut(s) 810
MaeI CTAG 1 cut(s) 647
MaeII ACGT 1 cut(s) 790
MaeIII GTNAC 4 cut(s) 42, 242, 953, 1024
MalI GATC 3 cut(s) 129, 587, 613
MboI GATC 3 cut(s) 127, 585, 611
MboII GAAGA 4 cut(s) 413, 635, 680, 1154
MfeI CAATTG 1 cut(s) 211
MflI RGATCY 2 cut(s) 585, 611
MhlI GDGCHC 1 cut(s) 1220
MluCI AATT 5 cut(s) 78, 139, 211, 935, 1210
MlyI GAGTC 5 cut(s) 190, 282, 352, 764, 902
MmeI TCCRAC 1 cut(s) 287
MnlI CCTC 8 cut(s) 5, 13, 144, 185, 263, 446, 543, 1212
Mph1103I ATGCAT 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 1 cut(s) 492
MspI CCGG 2 cut(s) 392, 827
MspR9I CCNGG 3 cut(s) 537, 827, 1229
MunI CAATTG 1 cut(s) 211
MvaI CCWGG 2 cut(s) 537, 1229
MwoI GCNNNNNNNGC 2 cut(s) 810, 1070
NciI CCSGG 1 cut(s) 827
NdeI CATATG 1 cut(s) 1069
NdeII GATC 3 cut(s) 127, 585, 611
NlaIII CATG 5 cut(s) 88, 350, 550, 994, 1032
NlaIV GGNNCC 4 cut(s) 390, 423, 613, 1054
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 1024
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 994
PciI ACATGT 1 cut(s) 990
PciSI GCTCTTC 1 cut(s) 1137
PcsI WCGNNNNNNNCGW 3 cut(s) 252, 261, 796
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 4 cut(s) 93, 184, 337, 563
Pfl23II CGTACG 1 cut(s) 411
PfoI TCCNGGA 2 cut(s) 535, 825
PkrI GCNGC 4 cut(s) 112, 815, 1075, 1078
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 5 cut(s) 190, 281, 351, 764, 902
PpsI GAGTC 5 cut(s) 190, 281, 351, 764, 902
PscI ACATGT 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 1220
Psp6I CCWGG 2 cut(s) 535, 1227
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 2 cut(s) 535, 1227
PspLI CGTACG 1 cut(s) 411
PspN4I GGNNCC 4 cut(s) 390, 423, 613, 1054
PspPI GGNCC 3 cut(s) 455, 1041, 1052
PstI CTGCAG 2 cut(s) 944, 1078
PsuI RGATCY 2 cut(s) 585, 611
PvuI CGATCG 1 cut(s) 130
RsaI GTAC 2 cut(s) 413, 509
RsaNI GTAC 2 cut(s) 412, 508
SacI GAGCTC 1 cut(s) 1220
SapI GCTCTTC 1 cut(s) 1137
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 4 cut(s) 111, 814, 1074, 1077
Sau3AI GATC 3 cut(s) 127, 585, 611
Sau96I GGNCC 3 cut(s) 455, 1041, 1052
ScaI AGTACT 1 cut(s) 509
SchI GAGTC 5 cut(s) 190, 282, 352, 764, 902
ScrFI CCNGG 3 cut(s) 537, 827, 1229
SduI GDGCHC 1 cut(s) 1220
SfaNI GCATC 1 cut(s) 810
SfcI CTRYAG 2 cut(s) 940, 1074
SinI GGWCC 2 cut(s) 1041, 1052
Sse9I AATT 5 cut(s) 78, 139, 211, 935, 1210
SsiI CCGC 6 cut(s) 111, 281, 473, 580, 814, 1050
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 1220
StyD4I CCNGG 3 cut(s) 535, 825, 1227
TaaI ACNGT 5 cut(s) 730, 761, 881, 1001, 1225
TaiI ACGT 1 cut(s) 793
TaqI TCGA 6 cut(s) 126, 182, 194, 298, 383, 911
TaqII GACCGA 1 cut(s) 92
TasI AATT 5 cut(s) 78, 139, 211, 935, 1210
TatI WGTACW 1 cut(s) 507
TauI GCSGC 2 cut(s) 113, 816
TfiI GAWTC 4 cut(s) 93, 184, 337, 563
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 2 cut(s) 851, 869
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 2 cut(s) 1073, 1076
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 7 cut(s) 73, 515, 516, 563, 1022, 1155, 1223
TspGWI ACGGA 4 cut(s) 282, 399, 505, 1139
TspRI CASTG 2 cut(s) 851, 869
VpaK11BI GGWCC 2 cut(s) 1041, 1052
XapI RAATTY 1 cut(s) 1210
XceI RCATGY 1 cut(s) 994
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 1 cut(s) 647
ZrmI AGTACT 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.