Prupe.1G583700_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012465
Physical Location & Seq
Reverse (-)
47402019 .. 47405368
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G583700.2

Sequence Viewer

Length: 1182 bp
ATGGCAAGAGGCAGGGGAGAGAGAGAAGACTATGAGAAGAACCCGGGTTTGCTCTCACTCTTGCAAGTTCTCTCATTCTTGGTTGTTTTTGTTGCTGGGGTCGTTATAGGATTAGCAACAAGCGCGCATATTAATCGGCATTTTGGTTCACAAACTGAGATTTACTCCTTCATTAATCACTTCTCCTCCAATAAGCCAAATGAGGAGGAAAATTGTACCATTGTGCAAGGTTGCGAGAAGGTTGATTGTTTGAGCATGGAGACGTTTCTTCAACCGAAGAATTTGACACACGATATGTCTGATGATGAGCTCTTTTGGAGAGCTTCAATGGTACCCGGGAAGGTAGAATTTCCATTCGAGAGAGTGCCTAAAGTGGCTTTCATGTTTTTGAGTAGAGGACCTTTGCCCATGATGCCATTGTGGGAGAGGTTTTTTCAAGGCCATGACAAGTTTTTCTCGATTTACGTGCACGTGCTTCCAGGGTATACGCTCAATGTATCTAGCACCTCTCCCTTTTATGGACGCCAAATTCCCAGTCAGCTTGTTTCATGGGGAACAGTAACACTAGTTGATGCAGAGAGGCGCCTGCTATCCAATGCCTTGCTTGACTTCTCAAATCAGCGCTTTGTTCTTCTCTCCGAGAGCTGCATCCCGGTCTATAATTTCCCTACCGTCTACAAGTATCTCATTGGCTCTAACCATAGCTTTGTTGAGTCGTACGACGATCCTGGCCGTTATGGACGTGGTCGTTACAGCCGAAAAATGCTTCCTGAGATTGAACTCTATCAATGGAGGAAAGGGTCTCAGTGGTTTGAACTTAGCCGTACGTTAGCAATTTACATAGTCTCAGAAACAAAGTACTACACCCTCTTCAGCAGGTATTGCTTGCCTGCTTGCTACCCGGATGAGCATTACATGCCAACTTACTTCAACATGTTCCATGGATCGCTAAACTCAAATCGGACTGTGACTTGGGTTGACTGGTCGCTGGGAGGTCCGCACCCTGCAACATACGGGGGAGATAACATTACAGAAGATTTTGTACGGTCAATACGAAACAGTGGAGCACTATGTCAGTACAATTCAGAGATGACATCTATTTGTTACCTATTTGCTAGGAAGTTTGCACCAAGTGCACTGGAGCCATTGCTGGAGCTGGCCTCATCAGTGATGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

394

Amino Acids

45.21

Weight (kDa)

6.08

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 867
Acc65I GGTACC 1 cut(s) 331
AccB1I GGYRCC 2 cut(s) 331, 582
AccI GTMKAC 2 cut(s) 485, 675
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 998
AclWI GGATC 2 cut(s) 719, 952
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 4 cut(s) 280, 347, 528, 1175
AcuI CTGAAG 1 cut(s) 856
AcvI CACGTG 1 cut(s) 472
AcyI GRCGYC 2 cut(s) 523, 583
AdeI CACNNNGTG 1 cut(s) 1133
AfaI GTAC 7 cut(s) 217, 333, 719, 826, 860, 1044, 1079
AfeI AGCGCT 1 cut(s) 623
AfiI CCNNNNNNNGG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 933
AgsI TTSAA 6 cut(s) 272, 327, 437, 779, 815, 931
AhlI ACTAGT 1 cut(s) 565
AjiI CACGTC 1 cut(s) 743
AjnI CCWGG 2 cut(s) 478, 727
AluBI AGCT 6 cut(s) 310, 323, 541, 645, 705, 1156
AluI AGCT 6 cut(s) 310, 323, 541, 645, 705, 1156
Alw21I GWGCWC 4 cut(s) 312, 471, 1069, 1138
Alw26I GTCTC 3 cut(s) 254, 807, 850
Alw44I GTGCAC 2 cut(s) 467, 1134
AlwI GGATC 2 cut(s) 719, 952
Ama87I CYCGRG 2 cut(s) 43, 335
Aor51HI AGCGCT 1 cut(s) 623
AoxI GGCC 3 cut(s) 439, 730, 1158
ApaLI GTGCAC 2 cut(s) 467, 1134
ApeKI GCWGC 1 cut(s) 645
ApoI RAATTY 4 cut(s) 280, 347, 528, 1175
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
AseI ATTAAT 2 cut(s) 132, 174
Asp718I GGTACC 1 cut(s) 331
AspLEI GCGC 4 cut(s) 125, 127, 585, 624
AspS9I GGNCC 2 cut(s) 398, 995
AsuC2I CCSGG 6 cut(s) 44, 45, 336, 337, 653, 902
AvaI CYCGRG 2 cut(s) 43, 335
AvaII GGWCC 2 cut(s) 398, 995
BaeGI GKGCMC 2 cut(s) 471, 1138
BanI GGYRCC 2 cut(s) 331, 582
BanII GRGCYC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 1026, 1058
BbrPI CACGTG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 4 cut(s) 312, 471, 1069, 1138
BbvI GCAGC 1 cut(s) 632
BccI CCATC 1 cut(s) 1165
BceAI ACGGC 2 cut(s) 717, 807
BcgI CGANNNNNNTGC 4 cut(s) 116, 150, 448, 482
BciT130I CCWGG 2 cut(s) 480, 729
BcnI CCSGG 6 cut(s) 44, 45, 336, 337, 653, 902
BcoDI GTCTC 3 cut(s) 254, 807, 850
BcuI ACTAGT 1 cut(s) 565
BfaI CTAG 3 cut(s) 501, 566, 1116
BfoI RGCGCY 2 cut(s) 586, 625
BfuAI ACCTGC 1 cut(s) 867
BisI GCNGC 1 cut(s) 646
BlsI GCNGC 1 cut(s) 647
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 8 cut(s) 44, 45, 336, 337, 480, 653, 729, 902
Bme18I GGWCC 2 cut(s) 398, 995
BmeT110I CYCGRG 2 cut(s) 43, 335
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 2 cut(s) 398, 995
BmiI GGNNCC 3 cut(s) 333, 584, 1143
BmrFI CCNGG 8 cut(s) 44, 45, 336, 337, 480, 653, 729, 902
BmrI ACTGGG 1 cut(s) 528
BmsI GCATC 3 cut(s) 402, 562, 657
BmuI ACTGGG 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 1145, 1177
BpmI CTGGAG 2 cut(s) 1160, 1172
BpuMI CCSGG 6 cut(s) 44, 45, 336, 337, 653, 902
BsaAI YACGTR 2 cut(s) 466, 472
BsaHI GRCGYC 2 cut(s) 523, 583
BsaI GGTCTC 1 cut(s) 807
BsaJI CCNNGG 4 cut(s) 43, 335, 479, 940
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse1I ACTGG 3 cut(s) 534, 986, 1143
Bse3DI GCAATG 1 cut(s) 1145
BseBI CCWGG 2 cut(s) 480, 729
BseDI CCNNGG 4 cut(s) 43, 335, 479, 940
BseGI GGATG 2 cut(s) 648, 910
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMI GCAATG 1 cut(s) 1145
BseMII CTCAG 4 cut(s) 147, 762, 818, 861
BseNI ACTGG 3 cut(s) 534, 986, 1143
BsePI GCGCGC 1 cut(s) 123
BseRI GAGGAG 2 cut(s) 175, 218
BseSI GKGCMC 2 cut(s) 471, 1138
BseXI GCAGC 1 cut(s) 632
BseYI CCCAGC 2 cut(s) 95, 988
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 3 cut(s) 441, 732, 1160
BshNI GGYRCC 2 cut(s) 331, 582
BsiHKAI GWGCWC 4 cut(s) 312, 471, 1069, 1138
BsiHKCI CYCGRG 2 cut(s) 43, 335
BsiSI CCGG 4 cut(s) 44, 336, 653, 902
BsiWI CGTACG 2 cut(s) 717, 824
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 3 cut(s) 254, 807, 850
BsmBI CGTCTC 1 cut(s) 254
BsnI GGCC 3 cut(s) 441, 732, 1160
Bso31I GGTCTC 1 cut(s) 807
BsoBI CYCGRG 2 cut(s) 43, 335
Bsp1286I GDGCHC 4 cut(s) 312, 471, 1069, 1138
Bsp143I GATC 2 cut(s) 724, 944
Bsp19I CCATGG 1 cut(s) 940
BspACI CCGC 1 cut(s) 998
BspANI GGCC 3 cut(s) 441, 732, 1160
BspCNI CTCAG 4 cut(s) 148, 763, 817, 860
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 3 cut(s) 333, 584, 1143
BspMI ACCTGC 1 cut(s) 867
BspPI GGATC 2 cut(s) 719, 952
BspT107I GGYRCC 2 cut(s) 331, 582
BspTNI GGTCTC 1 cut(s) 807
BsrDI GCAATG 1 cut(s) 1145
BsrI ACTGG 3 cut(s) 534, 986, 1143
BssECI CCNNGG 4 cut(s) 43, 335, 479, 940
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 2 cut(s) 724, 944
BssNAI GTATAC 1 cut(s) 486
BssNI GRCGYC 2 cut(s) 523, 583
BssT1I CCWWGG 1 cut(s) 940
Bst1107I GTATAC 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 480, 729
Bst4CI ACNGT 5 cut(s) 559, 673, 967, 1047, 1061
Bst6I CTCTTC 1 cut(s) 875
BstACI GRCGYC 2 cut(s) 523, 583
BstAPI GCANNNNNTGC 3 cut(s) 882, 916, 1133
BstBAI YACGTR 2 cut(s) 466, 472
BstC8I GCNNGC 6 cut(s) 125, 587, 887, 891, 895, 1158
BstDEI CTNAG 5 cut(s) 156, 771, 804, 818, 847
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 2 cut(s) 648, 910
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 586, 625
BstHHI GCGC 4 cut(s) 125, 127, 585, 624
BstKTI GATC 2 cut(s) 727, 947
BstMAI GTCTC 3 cut(s) 254, 807, 850
BstMBI GATC 2 cut(s) 724, 944
BstMWI GCNNNNNNNGC 5 cut(s) 122, 412, 882, 916, 1133
BstNI CCWGG 2 cut(s) 480, 729
BstNSI RCATGY 2 cut(s) 919, 937
BstSCI CCNGG 8 cut(s) 42, 43, 334, 335, 478, 651, 727, 900
BstSLI GKGCMC 2 cut(s) 471, 1138
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 1 cut(s) 632
BstV2I GAAGAC 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 486
BsuRI GGCC 3 cut(s) 441, 732, 1160
BtgI CCRYGG 1 cut(s) 940
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 2 cut(s) 648, 910
BtsIMutI CAGTG 4 cut(s) 812, 1066, 1136, 1173
BveI ACCTGC 1 cut(s) 867
Cac8I GCNNGC 6 cut(s) 125, 587, 887, 891, 895, 1158
CfoI GCGC 4 cut(s) 125, 127, 585, 624
Cfr13I GGNCC 2 cut(s) 398, 995
Cfr9I CCCGGG 2 cut(s) 43, 335
CseI GACGC 1 cut(s) 531
Csp6I GTAC 7 cut(s) 216, 332, 718, 825, 859, 1043, 1078
CviAII CATG 8 cut(s) 256, 382, 409, 443, 549, 916, 934, 941
CviQI GTAC 7 cut(s) 216, 332, 718, 825, 859, 1043, 1078
DdeI CTNAG 5 cut(s) 156, 771, 804, 818, 847
DinI GGCGCC 1 cut(s) 584
DpnI GATC 2 cut(s) 726, 946
DpnII GATC 2 cut(s) 724, 944
DraIII CACNNNGTG 1 cut(s) 1133
EaeI YGGCCR 1 cut(s) 730
Eam1104I CTCTTC 1 cut(s) 875
EarI CTCTTC 1 cut(s) 875
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 940
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 807
Eco47I GGWCC 2 cut(s) 398, 995
Eco47III AGCGCT 1 cut(s) 623
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 856
Eco72I CACGTG 1 cut(s) 472
Eco88I CYCGRG 2 cut(s) 43, 335
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 478, 727
EcoT14I CCWWGG 1 cut(s) 940
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 584
EheI GGCGCC 1 cut(s) 584
ErhI CCWWGG 1 cut(s) 940
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 8 cut(s) 259, 385, 412, 446, 552, 919, 937, 944
FatI CATG 8 cut(s) 255, 381, 408, 442, 548, 915, 933, 940
FblI GTMKAC 2 cut(s) 485, 675
Fnu4HI GCNGC 1 cut(s) 646
FokI GGATG 2 cut(s) 635, 917
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 646
FspBI CTAG 3 cut(s) 501, 566, 1116
GlaI GCGC 4 cut(s) 124, 126, 584, 623
GluI GCNGC 1 cut(s) 646
GsaI CCCAGC 2 cut(s) 99, 992
GsuI CTGGAG 2 cut(s) 1160, 1172
HaeII RGCGCY 2 cut(s) 586, 625
HaeIII GGCC 3 cut(s) 441, 732, 1160
HapII CCGG 4 cut(s) 44, 336, 653, 902
HgaI GACGC 1 cut(s) 531
HhaI GCGC 4 cut(s) 125, 127, 585, 624
Hin1I GRCGYC 2 cut(s) 523, 583
Hin1II CATG 8 cut(s) 259, 385, 412, 446, 552, 919, 937, 944
Hin6I GCGC 4 cut(s) 123, 125, 583, 622
HinP1I GCGC 4 cut(s) 123, 125, 583, 622
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HinfI GANTC 1 cut(s) 713
HpaII CCGG 4 cut(s) 44, 336, 653, 902
Hpy166II GTNNAC 6 cut(s) 149, 469, 486, 676, 979, 1136
Hpy188I TCNGA 5 cut(s) 301, 640, 850, 963, 1087
Hpy188III TCNNGA 3 cut(s) 358, 457, 770
Hpy8I GTNNAC 6 cut(s) 149, 469, 486, 676, 979, 1136
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 3 cut(s) 178, 232, 334
HpyCH4III ACNGT 5 cut(s) 559, 673, 967, 1047, 1061
HpyCH4IV ACGT 5 cut(s) 263, 465, 471, 742, 827
HpyCH4V TGCA 8 cut(s) 64, 226, 469, 575, 648, 1007, 1127, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 412, 882, 916, 1133
HpyF3I CTNAG 5 cut(s) 156, 771, 804, 818, 847
HpySE526I ACGT 5 cut(s) 263, 465, 471, 742, 827
Hsp92I GRCGYC 2 cut(s) 523, 583
Hsp92II CATG 8 cut(s) 259, 385, 412, 446, 552, 919, 937, 944
HspAI GCGC 4 cut(s) 123, 125, 583, 622
KasI GGCGCC 1 cut(s) 582
KpnI GGTACC 1 cut(s) 335
Kzo9I GATC 2 cut(s) 724, 944
LmnI GCTCC 3 cut(s) 1064, 1141, 1153
Lsp1109I GCAGC 1 cut(s) 632
LweI GCATC 3 cut(s) 402, 562, 657
MaeI CTAG 3 cut(s) 501, 566, 1116
MaeII ACGT 5 cut(s) 263, 465, 471, 742, 827
MaeIII GTNAC 4 cut(s) 559, 749, 967, 1103
MalI GATC 2 cut(s) 726, 946
MboI GATC 2 cut(s) 724, 944
MboII GAAGA 7 cut(s) 38, 49, 260, 289, 623, 862, 1046
MhlI GDGCHC 4 cut(s) 312, 471, 1069, 1138
MluCI AATT 8 cut(s) 211, 280, 347, 528, 661, 834, 1081, 1175
Mly113I GGCGCC 1 cut(s) 583
MlyI GAGTC 1 cut(s) 722
MseI TTAA 2 cut(s) 132, 174
MspI CCGG 4 cut(s) 44, 336, 653, 902
MspR9I CCNGG 8 cut(s) 44, 45, 336, 337, 480, 653, 729, 902
MvaI CCWGG 2 cut(s) 480, 729
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 122, 412, 882, 916, 1133
NarI GGCGCC 1 cut(s) 583
NciI CCSGG 6 cut(s) 44, 45, 336, 337, 653, 902
NcoI CCATGG 1 cut(s) 940
NdeII GATC 2 cut(s) 724, 944
NlaIII CATG 8 cut(s) 259, 385, 412, 446, 552, 919, 937, 944
NlaIV GGNNCC 3 cut(s) 333, 584, 1143
NmuCI GTSAC 1 cut(s) 967
NspI RCATGY 2 cut(s) 919, 937
PauI GCGCGC 1 cut(s) 123
PciI ACATGT 1 cut(s) 933
PcsI WCGNNNNNNNCGW 2 cut(s) 754, 1051
Pfl23II CGTACG 2 cut(s) 717, 824
PflFI GACNNNGTC 1 cut(s) 744
PkrI GCNGC 1 cut(s) 647
PleI GAGTC 1 cut(s) 721
PluTI GGCGCC 1 cut(s) 586
PmaCI CACGTG 1 cut(s) 472
PmlI CACGTG 1 cut(s) 472
PpsI GAGTC 1 cut(s) 721
Ppu21I YACGTR 2 cut(s) 466, 472
PpuMI RGGWCCY 1 cut(s) 398
PscI ACATGT 1 cut(s) 933
PshBI ATTAAT 2 cut(s) 132, 174
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 2 cut(s) 478, 727
PspCI CACGTG 1 cut(s) 472
PspFI CCCAGC 2 cut(s) 95, 988
PspGI CCWGG 2 cut(s) 478, 727
PspLI CGTACG 2 cut(s) 717, 824
PspN4I GGNNCC 3 cut(s) 333, 584, 1143
PspPI GGNCC 2 cut(s) 398, 995
PspPPI RGGWCCY 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 744
PteI GCGCGC 1 cut(s) 123
RsaI GTAC 7 cut(s) 217, 333, 719, 826, 860, 1044, 1079
RsaNI GTAC 7 cut(s) 216, 332, 718, 825, 859, 1043, 1078
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 132, 174
SatI GCNGC 1 cut(s) 646
Sau3AI GATC 2 cut(s) 724, 944
Sau96I GGNCC 2 cut(s) 398, 995
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 8 cut(s) 44, 45, 336, 337, 480, 653, 729, 902
SduI GDGCHC 4 cut(s) 312, 471, 1069, 1138
SfaNI GCATC 3 cut(s) 402, 562, 657
SfoI GGCGCC 1 cut(s) 584
SinI GGWCC 2 cut(s) 398, 995
SmaI CCCGGG 2 cut(s) 45, 337
SpeI ACTAGT 1 cut(s) 565
Sse9I AATT 8 cut(s) 211, 280, 347, 528, 661, 834, 1081, 1175
SsiI CCGC 1 cut(s) 998
SspDI GGCGCC 1 cut(s) 582
SspMI CTAG 3 cut(s) 501, 566, 1116
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 8 cut(s) 42, 43, 334, 335, 478, 651, 727, 900
StyI CCWWGG 1 cut(s) 940
TaaI ACNGT 5 cut(s) 559, 673, 967, 1047, 1061
TaiI ACGT 5 cut(s) 266, 468, 474, 745, 830
TaqI TCGA 2 cut(s) 357, 458
TasI AATT 8 cut(s) 211, 280, 347, 528, 661, 834, 1081, 1175
TatI WGTACW 2 cut(s) 858, 1077
Tru1I TTAA 2 cut(s) 132, 174
Tru9I TTAA 2 cut(s) 132, 174
TscAI CASTG 4 cut(s) 812, 1066, 1143, 1173
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 1 cut(s) 645
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 3 cut(s) 160, 370, 537
TspMI CCCGGG 2 cut(s) 43, 335
TspRI CASTG 4 cut(s) 812, 1066, 1143, 1173
Tth111I GACNNNGTC 1 cut(s) 744
VneI GTGCAC 2 cut(s) 467, 1134
VpaK11BI GGWCC 2 cut(s) 398, 995
VspI ATTAAT 2 cut(s) 132, 174
XapI RAATTY 4 cut(s) 280, 347, 528, 1175
XceI RCATGY 2 cut(s) 919, 937
XmaI CCCGGG 2 cut(s) 43, 335
XmiI GTMKAC 2 cut(s) 485, 675
XspI CTAG 3 cut(s) 501, 566, 1116
ZrmI AGTACT 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.