RLG00000002256

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012465
Physical Location & Seq
Forward (+)
30765909 .. 30768577
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002256

Sequence Viewer

Length: 1212 bp
ATGGCAAGAAGCAGATGGGAGAAAGAAGACTGCTACGATAAGAGCACGGGTTTGATCAAACTCTTGCAAGTTCTCTCCTTTTTGGTTGTTTTTGTTGTTGGTGTGGTTATTGGATTAGCAACAAGTGCCCACATTAATCGTCATTTCGGTGCACAAACTGAGATTTATTCGCTTATCAATCACTTCTCCTCCTCCTCCTCCTCCTTCCCCAAGGAGACACCACGCCAACAAGGGGAGCCTAATTGTACCGTTGTGCAAAGTTGCGAGAGGGTTGATTGTTTGAGCATGGAGACATTTCTGGAGCCTAAGAACTTAACACATGGTATGCCGGATAATGAGCTGTTTTGGAGAGCTTCAATGGTTCCCGCCAAGAAAGAATATCCATATCCGAGAGTGCCTAAAGTGGCTTTCATGTTTATGACTAGAGGACCGTTGCCATTGATGCCATTGTGGGAGAGGTTCTTTCAGGGACACGACAAGCTTTTCTCAGTTTATGTGCATGTAGTTCCAGGGTATAAGCTCAATGCATCAACCACCTCTCCTTTTTATCGACGCCAAATCCCTAGTCAGCCTGTTTCTTGGGGAACCATTACACTGATTGATGCAGAGAGGCGCCTTCTAGCTAATGCCTTGCTTGATTTCTCAAATGAGTGTTTTGTCCTCCTATCTGAGAGTTGCATCCCAGTCTATAACTTCCCTACAGTGTACAAGTATCTCATTGGTGCTAATTATAGCTTTGTTGAATCATACGACGATCCTGGACGTTATGGCCGTGGGCGTTACAGCCGTAAAATGCTTCCCAATATTCAGCTGTATCAGTGGAGGAAGGGGTCTCAGTGGTTTGAACTGAGCCGCACTTTAGCTACTTTAATAGTCTCAGAAGTACGGTACTACACCGTCTTCAGCAAATATTGTTTGCCTGCTTGCTACCCAGATGAGCATTACATGCCAACTTACTTCAATATGTTCCATGGTTCACTGAACTCTAATCGGACTGTGACTTGGGTAGACTGGTCTTTGGGAGGACCCCACCCTGCAACATACGGTGGAGATAATATTACAGAAGATTTTATACGGTCTATAAGGAATAATGGAGCACTTTGTCAGTACAATTCAGAGATGACATCCATTTGTTACCTATTTGCTAGGAAGTTTGCTCCAACAGCATTGGAGCCTTTGCTCGAGCTTGCCTCATCCGTGATGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

404

Amino Acids

46.35

Weight (kDa)

8.23

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 135 - 362 1.7e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 1008
AciI CCGC 2 cut(s) 366, 853
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 1 cut(s) 1205
AcuI CTGAAG 1 cut(s) 886
AcyI GRCGYC 2 cut(s) 553, 613
AfaI GTAC 5 cut(s) 247, 707, 885, 890, 1109
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 4 cut(s) 357, 743, 845, 961
AjnI CCWGG 2 cut(s) 508, 757
AluBI AGCT 9 cut(s) 340, 353, 481, 520, 623, 735, 811, 863, 1186
AluI AGCT 9 cut(s) 340, 353, 481, 520, 623, 735, 811, 863, 1186
Alw21I GWGCWC 3 cut(s) 47, 154, 1099
Alw26I GTCTC 4 cut(s) 209, 284, 837, 880
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 1181
AoxI GGCC 1 cut(s) 769
ApaLI GTGCAC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 1205
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AseI ATTAAT 1 cut(s) 135
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 2 cut(s) 428, 1025
AvaI CYCGRG 1 cut(s) 1181
AvaII GGWCC 2 cut(s) 428, 1025
BaeGI GKGCMC 2 cut(s) 130, 154
BanI GGYRCC 1 cut(s) 612
BarI GAAGNNNNNNTAC 2 cut(s) 1056, 1088
BbsI GAAGAC 2 cut(s) 33, 892
Bbv12I GWGCWC 3 cut(s) 47, 154, 1099
BccI CCATC 2 cut(s) 9, 1195
BceAI ACGGC 2 cut(s) 756, 771
BciT130I CCWGG 2 cut(s) 510, 759
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 4 cut(s) 209, 284, 837, 880
BfaI CTAG 4 cut(s) 423, 564, 620, 1146
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 616
BisI GCNGC 1 cut(s) 853
BlsI GCNGC 1 cut(s) 854
Bme1390I CCNGG 2 cut(s) 510, 759
Bme18I GGWCC 2 cut(s) 428, 1025
BmeT110I CYCGRG 1 cut(s) 1181
BmgT120I GGNCC 2 cut(s) 428, 1025
BmiI GGNNCC 7 cut(s) 237, 303, 363, 586, 614, 1027, 1173
BmrFI CCNGG 2 cut(s) 510, 759
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 4 cut(s) 432, 536, 592, 687
BmuI ACTGGG 1 cut(s) 677
BpiI GAAGAC 2 cut(s) 33, 892
BplI GAGNNNNNCTC 2 cut(s) 1175, 1207
BpmI CTGGAG 1 cut(s) 320
BsaHI GRCGYC 2 cut(s) 553, 613
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 210, 509, 772, 970
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 523, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 683, 1016
BseBI CCWGG 2 cut(s) 510, 759
BseDI CCNNGG 4 cut(s) 210, 509, 772, 970
BseGI GGATG 3 cut(s) 678, 1124, 1193
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 6 cut(s) 150, 501, 660, 839, 848, 891
BseNI ACTGG 2 cut(s) 683, 1016
BseRI GAGGAG 5 cut(s) 178, 181, 184, 187, 190
BseSI GKGCMC 2 cut(s) 130, 154
BshFI GGCC 1 cut(s) 771
BshNI GGYRCC 1 cut(s) 612
BsiHKAI GWGCWC 3 cut(s) 47, 154, 1099
BsiHKCI CYCGRG 1 cut(s) 1181
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 4 cut(s) 209, 284, 837, 880
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 1 cut(s) 771
Bso31I GGTCTC 1 cut(s) 837
BsoBI CYCGRG 1 cut(s) 1181
Bsp1286I GDGCHC 4 cut(s) 47, 130, 154, 1099
Bsp1407I TGTACA 1 cut(s) 705
Bsp143I GATC 2 cut(s) 54, 754
Bsp19I CCATGG 1 cut(s) 970
BspACI CCGC 2 cut(s) 366, 853
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 6 cut(s) 151, 500, 661, 840, 847, 890
BspLI GGNNCC 7 cut(s) 237, 303, 363, 586, 614, 1027, 1173
BspPI GGATC 1 cut(s) 749
BspT107I GGYRCC 1 cut(s) 612
BspTNI GGTCTC 1 cut(s) 837
BsrGI TGTACA 1 cut(s) 705
BsrI ACTGG 2 cut(s) 683, 1016
BssECI CCNNGG 4 cut(s) 210, 509, 772, 970
BssMI GATC 2 cut(s) 54, 754
BssNI GRCGYC 2 cut(s) 553, 613
BssT1I CCWWGG 2 cut(s) 210, 970
Bst2UI CCWGG 2 cut(s) 510, 759
Bst4CI ACNGT 8 cut(s) 250, 432, 703, 888, 898, 997, 1046, 1077
BstACI GRCGYC 2 cut(s) 553, 613
BstAPI GCANNNNNTGC 2 cut(s) 125, 946
BstAUI TGTACA 1 cut(s) 705
BstC8I GCNNGC 3 cut(s) 921, 925, 1188
BstDEI CTNAG 7 cut(s) 159, 306, 487, 669, 834, 848, 877
BstDSI CCRYGG 2 cut(s) 772, 970
BstF5I GGATG 3 cut(s) 678, 1124, 1193
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 2 cut(s) 57, 757
BstMAI GTCTC 4 cut(s) 209, 284, 837, 880
BstMBI GATC 2 cut(s) 54, 754
BstMWI GCNNNNNNNGC 4 cut(s) 125, 442, 946, 1163
BstNI CCWGG 2 cut(s) 510, 759
BstNSI RCATGY 2 cut(s) 503, 949
BstSCI CCNGG 2 cut(s) 508, 757
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 2 cut(s) 130, 154
BstV2I GAAGAC 2 cut(s) 33, 892
BsuRI GGCC 1 cut(s) 771
BtgI CCRYGG 2 cut(s) 772, 970
BtsCI GGATG 3 cut(s) 678, 1124, 1193
BtsIMutI CAGTG 5 cut(s) 593, 708, 824, 842, 977
Cac8I GCNNGC 3 cut(s) 921, 925, 1188
CfoI GCGC 1 cut(s) 615
Cfr13I GGNCC 2 cut(s) 428, 1025
CseI GACGC 1 cut(s) 561
Csp6I GTAC 5 cut(s) 246, 706, 884, 889, 1108
CviAII CATG 6 cut(s) 286, 320, 412, 500, 946, 971
CviQI GTAC 5 cut(s) 246, 706, 884, 889, 1108
DdeI CTNAG 7 cut(s) 159, 306, 487, 669, 834, 848, 877
DinI GGCGCC 1 cut(s) 614
DpnI GATC 2 cut(s) 56, 756
DpnII GATC 2 cut(s) 54, 754
EaeI YGGCCR 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 210, 970
Eco31I GGTCTC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 428, 1025
Eco57I CTGAAG 1 cut(s) 886
Eco88I CYCGRG 1 cut(s) 1181
EcoO109I RGGNCCY 1 cut(s) 1025
EcoRII CCWGG 2 cut(s) 508, 757
EcoT14I CCWWGG 2 cut(s) 210, 970
EcoT22I ATGCAT 1 cut(s) 529
EgeI GGCGCC 1 cut(s) 614
EheI GGCGCC 1 cut(s) 614
ErhI CCWWGG 2 cut(s) 210, 970
FaeI CATG 6 cut(s) 289, 323, 415, 503, 949, 974
FaqI GGGAC 1 cut(s) 483
FatI CATG 6 cut(s) 285, 319, 411, 499, 945, 970
FauI CCCGC 1 cut(s) 373
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 1008
Fnu4HI GCNGC 1 cut(s) 853
FokI GGATG 3 cut(s) 665, 1111, 1180
Fsp4HI GCNGC 1 cut(s) 853
FspBI CTAG 4 cut(s) 423, 564, 620, 1146
GlaI GCGC 1 cut(s) 614
GluI GCNGC 1 cut(s) 853
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 1 cut(s) 771
HapII CCGG 1 cut(s) 329
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 615
Hin1I GRCGYC 2 cut(s) 553, 613
Hin1II CATG 6 cut(s) 289, 323, 415, 503, 949, 974
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 1 cut(s) 743
HpaII CCGG 1 cut(s) 329
Hpy166II GTNNAC 4 cut(s) 152, 706, 977, 1009
Hpy188I TCNGA 5 cut(s) 390, 670, 880, 993, 1117
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 4 cut(s) 152, 706, 977, 1009
Hpy99I CGWCG 2 cut(s) 555, 755
HpyAV CCTTC 3 cut(s) 214, 626, 820
HpyCH4III ACNGT 8 cut(s) 250, 432, 703, 888, 898, 997, 1046, 1077
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 8 cut(s) 67, 152, 256, 499, 527, 605, 678, 1037
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 442, 946, 1163
HpyF3I CTNAG 7 cut(s) 159, 306, 487, 669, 834, 848, 877
HpySE526I ACGT 1 cut(s) 763
Hsp92I GRCGYC 2 cut(s) 553, 613
Hsp92II CATG 6 cut(s) 289, 323, 415, 503, 949, 974
HspAI GCGC 1 cut(s) 613
KasI GGCGCC 1 cut(s) 612
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 2 cut(s) 54, 754
LmnI GCTCC 5 cut(s) 235, 301, 1094, 1162, 1171
LweI GCATC 4 cut(s) 432, 536, 592, 687
MaeI CTAG 4 cut(s) 423, 564, 620, 1146
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 3 cut(s) 779, 997, 1133
MalI GATC 2 cut(s) 56, 756
MboI GATC 2 cut(s) 54, 754
MboII GAAGA 3 cut(s) 38, 892, 1076
MhlI GDGCHC 4 cut(s) 47, 130, 154, 1099
MluCI AATT 4 cut(s) 241, 727, 1111, 1205
Mly113I GGCGCC 1 cut(s) 613
MmeI TCCRAC 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 3 cut(s) 135, 314, 869
MslI CAYNNNNRTG 2 cut(s) 147, 416
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 2 cut(s) 510, 759
MvaI CCWGG 2 cut(s) 510, 759
MwoI GCNNNNNNNGC 4 cut(s) 125, 442, 946, 1163
NarI GGCGCC 1 cut(s) 613
NcoI CCATGG 1 cut(s) 970
NdeII GATC 2 cut(s) 54, 754
NlaIII CATG 6 cut(s) 289, 323, 415, 503, 949, 974
NlaIV GGNNCC 7 cut(s) 237, 303, 363, 586, 614, 1027, 1173
NmuCI GTSAC 1 cut(s) 997
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 2 cut(s) 503, 949
PaeR7I CTCGAG 1 cut(s) 1181
PcsI WCGNNNNNNNCGW 1 cut(s) 769
PfeI GAWTC 1 cut(s) 743
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 1 cut(s) 854
PluTI GGCGCC 1 cut(s) 616
PpuMI RGGWCCY 1 cut(s) 1025
PshBI ATTAAT 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 2 cut(s) 508, 757
PspGI CCWGG 2 cut(s) 508, 757
PspN4I GGNNCC 7 cut(s) 237, 303, 363, 586, 614, 1027, 1173
PspPI GGNCC 2 cut(s) 428, 1025
PspPPI RGGWCCY 1 cut(s) 1025
PspXI VCTCGAGB 1 cut(s) 1181
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 5 cut(s) 247, 707, 885, 890, 1109
RsaNI GTAC 5 cut(s) 246, 706, 884, 889, 1108
RseI CAYNNNNRTG 2 cut(s) 147, 416
SaqAI TTAA 3 cut(s) 135, 314, 869
SatI GCNGC 1 cut(s) 853
Sau3AI GATC 2 cut(s) 54, 754
Sau96I GGNCC 2 cut(s) 428, 1025
ScrFI CCNGG 2 cut(s) 510, 759
SduI GDGCHC 4 cut(s) 47, 130, 154, 1099
SfaNI GCATC 4 cut(s) 432, 536, 592, 687
SfcI CTRYAG 1 cut(s) 699
SfoI GGCGCC 1 cut(s) 614
Sfr274I CTCGAG 1 cut(s) 1181
SinI GGWCC 2 cut(s) 428, 1025
SlaI CTCGAG 1 cut(s) 1181
SmiMI CAYNNNNRTG 2 cut(s) 147, 416
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
Sse9I AATT 4 cut(s) 241, 727, 1111, 1205
SsiI CCGC 2 cut(s) 366, 853
SspDI GGCGCC 1 cut(s) 612
SspI AATATT 3 cut(s) 805, 911, 1057
SspMI CTAG 4 cut(s) 423, 564, 620, 1146
StyD4I CCNGG 2 cut(s) 508, 757
StyI CCWWGG 2 cut(s) 210, 970
TaaI ACNGT 8 cut(s) 250, 432, 703, 888, 898, 997, 1046, 1077
TaiI ACGT 1 cut(s) 766
TaqI TCGA 2 cut(s) 550, 1182
TasI AATT 4 cut(s) 241, 727, 1111, 1205
TatI WGTACW 2 cut(s) 705, 1107
TauI GCSGC 1 cut(s) 855
TfiI GAWTC 1 cut(s) 743
Tru1I TTAA 3 cut(s) 135, 314, 869
Tru9I TTAA 3 cut(s) 135, 314, 869
TscAI CASTG 5 cut(s) 600, 708, 824, 842, 984
TseFI GTSAC 1 cut(s) 997
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 1 cut(s) 400
TspGWI ACGGA 1 cut(s) 1186
TspRI CASTG 5 cut(s) 600, 708, 824, 842, 984
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 428, 1025
VspI ATTAAT 1 cut(s) 135
XapI RAATTY 1 cut(s) 1205
XceI RCATGY 2 cut(s) 503, 949
XhoI CTCGAG 1 cut(s) 1181
XmiI GTMKAC 1 cut(s) 1008
XspI CTAG 4 cut(s) 423, 564, 620, 1146
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.