MD15G1441600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012465
Physical Location & Seq
Reverse (-)
54129476 .. 54131082
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1441600.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGCAAGAGGCAGAGAAGACTATGAGAAGAACCCGGGTTTGCTCAAACTCTTGCAGACTCTCTCATTCTTGATAGTTTTTGTTATGGGGGTCGTTATAGGATTAGCAACGAGGGCGCATATGAGCCGGTATTTTGGTTCCCAAACTGAGAACTATTCGTTTATCAATCACCTCTCTGCCAATATGCCGCACGAGGAGAAGACTTGTACAATTGTGCCAGTTAGTGACAAGGTTGATTGCATGAGCATGGAGACGTTTCTTCAGCCGAAGAATTTGACACACGGTCTGTCTGATGATGAGCTTTTTTGGAGAGCTTCAATGGTGCCGAGAAAGCGAGAGTTTCCATTCGAGAGAGTGCCTAAAGTGGCTTTCATGTTTTTGACTAGAGGACCTTTGCCCTTGCTGCCATTGTGGGAGAGGTTCTTTCAGGGCCATGACAAGTATTTCTCAATTTACGTCCACGTAATTCCGGGTTATAAGCTCAATGTATCTACCACCTCTCCTTTTTATCTACGCCAAATCCCCAGTCAGCCGGTTTCATGGGGGACAGTAACGTTGCTTGATGCAGAGAGGCGCCTTCTAGCGAATGCCTTGCTTGACTTCTCAAATGAGCGCTTTGTTCTTCTCTCGGAGAGCTGCATCCCGATCTATAATTTCACTACGGTCTACGAGTATCTCGTTGGTTCAAACCATAGCTATGTCGAGTCATACGATGATCCTGGGCGTTATGGCCGCGGGCGTTACAGGCGCAAAATGTTTCCCGATATTAAACTGCATCAGTGGAGGAAAGGGTCGCAGTGGTTTGAACTTAGCCGTGCGTTATCTGTATATATAGTTTCGGAAACAAAGTATTACACGATCTTCAGCAACTATTGTTTGCCTGCATGCTACCCGGATGAGCATTACCTGCCTACTTACATCAACATGCTCCAAGGGTCGCTGAATTCGAATCGGACTGTGACGTGGGTTGACTGGTCACTGCGAGGTCCGCACCCTGCAACGTACGGAGGAGACAACATTACAGAAGATTTCATGCAGTCGATAAGGAACAATGGAGAGCCGTGTCAGTACAATGCGGAGATGACATCCATATGTTACCTATTTGCTCGGAAGTTTGCACCAACCGCATTGGAGCCTCTGCTCAAGCTCGCCGCGGCGGTGATGGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

391

Amino Acids

45.26

Weight (kDa)

8.06

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 122 - 349 1.1e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
Acc36I ACCTGC 1 cut(s) 915
AccB1I GGYRCC 2 cut(s) 322, 573
AccI GTMKAC 1 cut(s) 666
AccII CGCG 2 cut(s) 735, 1154
AciI CCGC 9 cut(s) 188, 733, 735, 989, 1076, 1125, 1152, 1154, 1157
AclI AACGTT 1 cut(s) 554
AclWI GGATC 1 cut(s) 710
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 2 cut(s) 271, 943
AcuI CTGAAG 2 cut(s) 245, 847
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 3 cut(s) 208, 1004, 1070
AfeI AGCGCT 1 cut(s) 614
AgsI TTSAA 3 cut(s) 318, 687, 806
AhdI GACNNNNNGTC 1 cut(s) 282
AjiI CACGTC 1 cut(s) 963
AjnI CCWGG 1 cut(s) 718
AluBI AGCT 6 cut(s) 301, 314, 481, 636, 696, 1147
AluI AGCT 6 cut(s) 301, 314, 481, 636, 696, 1147
Alw26I GTCTC 2 cut(s) 245, 1005
AlwI GGATC 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 34
Aor51HI AGCGCT 1 cut(s) 614
AoxI GGCC 2 cut(s) 430, 730
ApeKI GCWGC 2 cut(s) 403, 636
ApoI RAATTY 2 cut(s) 271, 943
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
AspLEI GCGC 4 cut(s) 118, 576, 615, 750
AspS9I GGNCC 3 cut(s) 389, 430, 986
AsuC2I CCSGG 4 cut(s) 35, 36, 471, 893
AsuHPI GGTGA 2 cut(s) 161, 1171
AsuII TTCGAA 1 cut(s) 947
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 2 cut(s) 389, 986
BanI GGYRCC 2 cut(s) 322, 573
BauI CACGAG 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 24, 206
BbvI GCAGC 2 cut(s) 390, 623
BccI CCATC 1 cut(s) 1156
BceAI ACGGC 2 cut(s) 798, 1045
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BciT130I CCWGG 1 cut(s) 720
BcnI CCSGG 4 cut(s) 35, 36, 471, 893
BcoDI GTCTC 2 cut(s) 245, 1005
BfaI CTAG 2 cut(s) 384, 581
BfoI RGCGCY 2 cut(s) 577, 616
BfuAI ACCTGC 1 cut(s) 915
BisI GCNGC 6 cut(s) 188, 404, 637, 733, 1152, 1155
BlsI GCNGC 6 cut(s) 189, 405, 638, 734, 1153, 1156
Bme1390I CCNGG 5 cut(s) 35, 36, 471, 720, 893
Bme18I GGWCC 2 cut(s) 389, 986
BmeRI GACNNNNNGTC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 34
BmgBI CACGTC 1 cut(s) 963
BmgT120I GGNCC 3 cut(s) 389, 430, 986
BmiI GGNNCC 4 cut(s) 139, 324, 575, 1134
BmrFI CCNGG 5 cut(s) 35, 36, 471, 720, 893
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 3 cut(s) 553, 648, 784
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 2 cut(s) 24, 206
Bpu14I TTCGAA 1 cut(s) 947
BpuEI CTTGAG 1 cut(s) 1127
BpuMI CCSGG 4 cut(s) 35, 36, 471, 893
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 5 cut(s) 34, 719, 733, 931, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 484, 514
Bse118I RCCGGY 2 cut(s) 126, 532
Bse1I ACTGG 3 cut(s) 218, 525, 977
BseBI CCWGG 1 cut(s) 720
BseDI CCNNGG 5 cut(s) 34, 719, 733, 931, 1152
BseGI GGATG 3 cut(s) 639, 901, 1085
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 3 cut(s) 218, 525, 977
BseRI GAGGAG 2 cut(s) 209, 1023
BseXI GCAGC 2 cut(s) 390, 623
Bsh1236I CGCG 2 cut(s) 735, 1154
BshFI GGCC 2 cut(s) 432, 732
BshNI GGYRCC 2 cut(s) 322, 573
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 5 cut(s) 35, 127, 470, 533, 893
BsiWI CGTACG 1 cut(s) 1002
BslFI GGGAC 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 245, 1005
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 432, 732
BsoBI CYCGRG 1 cut(s) 34
Bsp119I TTCGAA 1 cut(s) 947
Bsp1407I TGTACA 1 cut(s) 206
Bsp143I GATC 3 cut(s) 645, 715, 858
BspACI CCGC 9 cut(s) 188, 733, 735, 989, 1076, 1125, 1152, 1154, 1157
BspANI GGCC 2 cut(s) 432, 732
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 2 cut(s) 735, 1154
BspLI GGNNCC 4 cut(s) 139, 324, 575, 1134
BspMI ACCTGC 1 cut(s) 915
BspPI GGATC 1 cut(s) 710
BspT104I TTCGAA 1 cut(s) 947
BspT107I GGYRCC 2 cut(s) 322, 573
BsrFI RCCGGY 2 cut(s) 126, 532
BsrGI TGTACA 1 cut(s) 206
BsrI ACTGG 3 cut(s) 218, 525, 977
BssAI RCCGGY 2 cut(s) 126, 532
BssECI CCNNGG 5 cut(s) 34, 719, 733, 931, 1152
BssMI GATC 3 cut(s) 645, 715, 858
BssNI GRCGYC 1 cut(s) 574
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 931
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 4 cut(s) 284, 550, 664, 958
BstACI GRCGYC 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 907
BstAUI TGTACA 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 463
BstBI TTCGAA 1 cut(s) 947
BstC8I GCNNGC 4 cut(s) 737, 882, 886, 1149
BstDEI CTNAG 2 cut(s) 147, 809
BstDSI CCRYGG 2 cut(s) 733, 1152
BstF5I GGATG 3 cut(s) 639, 901, 1085
BstFNI CGCG 2 cut(s) 735, 1154
BstH2I RGCGCY 2 cut(s) 577, 616
BstHHI GCGC 4 cut(s) 118, 576, 615, 750
BstKTI GATC 3 cut(s) 648, 718, 861
BstMAI GTCTC 2 cut(s) 245, 1005
BstMBI GATC 3 cut(s) 645, 715, 858
BstMWI GCNNNNNNNGC 7 cut(s) 113, 331, 403, 745, 907, 988, 1124
BstNI CCWGG 1 cut(s) 720
BstNSI RCATGY 2 cut(s) 888, 928
BstSCI CCNGG 5 cut(s) 33, 34, 469, 718, 891
BstUI CGCG 2 cut(s) 735, 1154
BstV1I GCAGC 2 cut(s) 390, 623
BstV2I GAAGAC 2 cut(s) 24, 206
BsuRI GGCC 2 cut(s) 432, 732
BtgI CCRYGG 2 cut(s) 733, 1152
BtrI CACGTC 1 cut(s) 963
BtsCI GGATG 3 cut(s) 639, 901, 1085
BtsI GCAGTG 2 cut(s) 803, 977
BtsIMutI CAGTG 3 cut(s) 785, 803, 977
BveI ACCTGC 1 cut(s) 915
Cac8I GCNNGC 4 cut(s) 737, 882, 886, 1149
CfoI GCGC 4 cut(s) 118, 576, 615, 750
Cfr10I RCCGGY 2 cut(s) 126, 532
Cfr13I GGNCC 3 cut(s) 389, 430, 986
Cfr42I CCGCGG 2 cut(s) 736, 1155
Cfr9I CCCGGG 1 cut(s) 34
Csp6I GTAC 3 cut(s) 207, 1003, 1069
CviAII CATG 8 cut(s) 241, 247, 373, 434, 540, 885, 925, 1033
CviQI GTAC 3 cut(s) 207, 1003, 1069
DdeI CTNAG 2 cut(s) 147, 809
DinI GGCGCC 1 cut(s) 575
DpnI GATC 3 cut(s) 647, 717, 860
DpnII GATC 3 cut(s) 645, 715, 858
DriI GACNNNNNGTC 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 730
Eam1105I GACNNNNNGTC 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 931
Eco47I GGWCC 2 cut(s) 389, 986
Eco47III AGCGCT 1 cut(s) 614
Eco57I CTGAAG 2 cut(s) 245, 847
Eco88I CYCGRG 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 943
EcoRII CCWGG 1 cut(s) 718
EcoT14I CCWWGG 1 cut(s) 931
EgeI GGCGCC 1 cut(s) 575
EheI GGCGCC 1 cut(s) 575
ErhI CCWWGG 1 cut(s) 931
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 8 cut(s) 244, 250, 376, 437, 543, 888, 928, 1036
FaqI GGGAC 1 cut(s) 559
FatI CATG 8 cut(s) 240, 246, 372, 433, 539, 884, 924, 1032
FauI CCCGC 1 cut(s) 728
FauNDI CATATG 2 cut(s) 120, 1091
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 6 cut(s) 188, 404, 637, 733, 1152, 1155
FokI GGATG 3 cut(s) 626, 908, 1072
Fsp4HI GCNGC 6 cut(s) 188, 404, 637, 733, 1152, 1155
FspBI CTAG 2 cut(s) 384, 581
GlaI GCGC 4 cut(s) 117, 575, 614, 749
GluI GCNGC 6 cut(s) 188, 404, 637, 733, 1152, 1155
HaeII RGCGCY 2 cut(s) 577, 616
HaeIII GGCC 2 cut(s) 432, 732
HapII CCGG 5 cut(s) 35, 127, 470, 533, 893
HhaI GCGC 4 cut(s) 118, 576, 615, 750
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 8 cut(s) 244, 250, 376, 437, 543, 888, 928, 1036
Hin6I GCGC 4 cut(s) 116, 574, 613, 748
HinP1I GCGC 4 cut(s) 116, 574, 613, 748
HincII GTYRAC 1 cut(s) 970
HindII GTYRAC 1 cut(s) 970
HinfI GANTC 3 cut(s) 58, 704, 949
HpaII CCGG 5 cut(s) 35, 127, 470, 533, 893
HphI GGTGA 2 cut(s) 161, 1171
Hpy166II GTNNAC 3 cut(s) 460, 667, 970
Hpy188I TCNGA 5 cut(s) 292, 631, 841, 954, 1110
Hpy188III TCNNGA 4 cut(s) 70, 349, 643, 761
Hpy8I GTNNAC 3 cut(s) 460, 667, 970
HpyAV CCTTC 1 cut(s) 587
HpyCH4III ACNGT 4 cut(s) 284, 550, 664, 958
HpyCH4IV ACGT 6 cut(s) 254, 456, 462, 554, 962, 1001
HpyCH4V TGCA 9 cut(s) 55, 240, 566, 639, 775, 884, 998, 1036, 1118
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 331, 403, 745, 907, 988, 1124
HpyF3I CTNAG 2 cut(s) 147, 809
HpySE526I ACGT 6 cut(s) 254, 456, 462, 554, 962, 1001
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 8 cut(s) 244, 250, 376, 437, 543, 888, 928, 1036
HspAI GCGC 4 cut(s) 116, 574, 613, 748
KasI GGCGCC 1 cut(s) 573
KspI CCGCGG 2 cut(s) 736, 1155
Kzo9I GATC 3 cut(s) 645, 715, 858
LmnI GCTCC 2 cut(s) 933, 1132
Lsp1109I GCAGC 2 cut(s) 390, 623
LweI GCATC 3 cut(s) 553, 648, 784
MaeI CTAG 2 cut(s) 384, 581
MaeII ACGT 6 cut(s) 254, 456, 462, 554, 962, 1001
MaeIII GTNAC 6 cut(s) 224, 550, 740, 958, 975, 1094
MalI GATC 3 cut(s) 647, 717, 860
MboI GATC 3 cut(s) 645, 715, 858
MboII GAAGA 8 cut(s) 29, 40, 211, 251, 280, 614, 853, 1037
MfeI CAATTG 1 cut(s) 210
MluCI AATT 6 cut(s) 210, 271, 450, 465, 652, 943
Mly113I GGCGCC 1 cut(s) 574
MlyI GAGTC 2 cut(s) 52, 713
MseI TTAA 1 cut(s) 768
MslI CAYNNNNRTG 4 cut(s) 245, 696, 923, 1090
MspA1I CMGCKG 2 cut(s) 735, 1154
MspI CCGG 5 cut(s) 35, 127, 470, 533, 893
MspR9I CCNGG 5 cut(s) 35, 36, 471, 720, 893
MunI CAATTG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 720
MvnI CGCG 2 cut(s) 735, 1154
MwoI GCNNNNNNNGC 7 cut(s) 113, 331, 403, 745, 907, 988, 1124
NarI GGCGCC 1 cut(s) 574
NciI CCSGG 4 cut(s) 35, 36, 471, 893
NdeI CATATG 2 cut(s) 120, 1091
NdeII GATC 3 cut(s) 645, 715, 858
NlaIII CATG 8 cut(s) 244, 250, 376, 437, 543, 888, 928, 1036
NlaIV GGNNCC 4 cut(s) 139, 324, 575, 1134
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 224, 958, 975
NspI RCATGY 2 cut(s) 888, 928
NspV TTCGAA 1 cut(s) 947
PaeI GCATGC 1 cut(s) 888
PcsI WCGNNNNNNNCGW 3 cut(s) 675, 708, 944
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 949
Pfl23II CGTACG 1 cut(s) 1002
PkrI GCNGC 6 cut(s) 189, 405, 638, 734, 1153, 1156
PleI GAGTC 2 cut(s) 52, 712
PluTI GGCGCC 1 cut(s) 577
PpsI GAGTC 2 cut(s) 52, 712
Ppu21I YACGTR 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 389
PsiI TTATAA 1 cut(s) 477
Psp1406I AACGTT 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 718
PspGI CCWGG 1 cut(s) 718
PspLI CGTACG 1 cut(s) 1002
PspN4I GGNNCC 4 cut(s) 139, 324, 575, 1134
PspPI GGNCC 3 cut(s) 389, 430, 986
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 3 cut(s) 208, 1004, 1070
RsaNI GTAC 3 cut(s) 207, 1003, 1069
RseI CAYNNNNRTG 4 cut(s) 245, 696, 923, 1090
SacII CCGCGG 2 cut(s) 736, 1155
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 6 cut(s) 188, 404, 637, 733, 1152, 1155
Sau3AI GATC 3 cut(s) 645, 715, 858
Sau96I GGNCC 3 cut(s) 389, 430, 986
SchI GAGTC 2 cut(s) 52, 713
ScrFI CCNGG 5 cut(s) 35, 36, 471, 720, 893
SfaNI GCATC 3 cut(s) 553, 648, 784
SfoI GGCGCC 1 cut(s) 575
Sfr303I CCGCGG 2 cut(s) 736, 1155
SfuI TTCGAA 1 cut(s) 947
SgrBI CCGCGG 2 cut(s) 736, 1155
SinI GGWCC 2 cut(s) 389, 986
SmaI CCCGGG 1 cut(s) 36
SmiMI CAYNNNNRTG 4 cut(s) 245, 696, 923, 1090
SmlI CTYRAG 1 cut(s) 1142
SmoI CTYRAG 1 cut(s) 1142
SphI GCATGC 1 cut(s) 888
Sse9I AATT 6 cut(s) 210, 271, 450, 465, 652, 943
SsiI CCGC 9 cut(s) 188, 733, 735, 989, 1076, 1125, 1152, 1154, 1157
SspDI GGCGCC 1 cut(s) 573
SspI AATATT 1 cut(s) 1169
SspMI CTAG 2 cut(s) 384, 581
StyD4I CCNGG 5 cut(s) 33, 34, 469, 718, 891
StyI CCWWGG 1 cut(s) 931
TaaI ACNGT 4 cut(s) 284, 550, 664, 958
TaiI ACGT 6 cut(s) 257, 459, 465, 557, 965, 1004
TaqI TCGA 4 cut(s) 348, 702, 947, 1040
TasI AATT 6 cut(s) 210, 271, 450, 465, 652, 943
TatI WGTACW 2 cut(s) 206, 1068
TauI GCSGC 4 cut(s) 190, 735, 1154, 1157
TfiI GAWTC 1 cut(s) 949
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 3 cut(s) 785, 803, 984
TseFI GTSAC 3 cut(s) 224, 958, 975
TseI GCWGC 2 cut(s) 403, 636
Tsp45I GTSAC 3 cut(s) 224, 958, 975
TspDTI ATGAA 3 cut(s) 361, 528, 1021
TspGWI ACGGA 1 cut(s) 1020
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 3 cut(s) 785, 803, 984
VpaK11BI GGWCC 2 cut(s) 389, 986
XapI RAATTY 2 cut(s) 271, 943
XceI RCATGY 2 cut(s) 888, 928
XmaI CCCGGG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 666
XspI CTAG 2 cut(s) 384, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.