Rmu_sc0016657.1_g000006

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016657.1
OG0012465
Physical Location & Seq
Reverse (-)
20159 .. 22961
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016657.1_g000006.1.cds

Sequence Viewer

Length: 1197 bp
atggcaagaagcagaggcgagaaagaagactgctacgataagagcacgggtttgatcaaactcttgcaggttctctcctttctggttgtttttgttgttggtgtggttattgggttggcaacaagtgcccacattaatcgtcatttcggtgcacaaaccgagatttattcgcttatcaatcacttctcctcctccaaggagacaccacaccaacaaggggagcctagttgtaccgttgcgcaaagttgcgagagggttgattgtttcagcatggagacatttctggagcctaagaacttgacacatggtatgacggataacgagctgttttggagagcttcaatggttccggcaacgaaagaatatccatatcagagagtgcctaaagtggctttcatgtttatgactagaggaccgttgccattgatgccattgtgggagaggttctttcagggacatgacaagcttttctcagtttatgtgcatgtagttccagggtataagctcaatgcatcaacaacctcccctttttatcgacgccaaatccctagtcagcctgtttcttggggaaccattacactgattgatgcagagaggcgccttctagctaatgccttgcttgatttctcaaatgagtgctttgtcctcctatctgagagttgcatcccagtctataactttcctacagtgtacaagtatctcattggtgctaattatagctttgttgaatcatacgacgatcctggacgttatggtcgtgggcgttacagccgtaaaatgcttcccgatattcagctgtatcagtggaggaagggatctcagtggtttgaacttagccgcactttagctactttaatagtctcagaagtacagtactacaccgtcttcagcaaatattgtttgcctgcttgctacccagatgagcattacatgccaacttacttcaatatgttccatggttcactgaactctaatcggactgtgacttgggtagactggtctttgggaggaccacaccctgcaacatatggtggagataatattacagaagattttatacggtctataaggaataatggagcactttgtcagtacaattcagagatgacatccatttgttacctatttgctaggaagtttgcgccaacagcattggagcctctgctcgagcttgcctcatccgtgatggaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

398

Amino Acids

45.65

Weight (kDa)

6.79

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 753
Acc16I TGCGCA 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 58
AccB1I GGYRCC 1 cut(s) 597
AccI GTMKAC 1 cut(s) 993
AciI CCGC 1 cut(s) 838
AclWI GGATC 2 cut(s) 734, 823
AcsI RAATTY 1 cut(s) 1190
AcuI CTGAAG 1 cut(s) 871
AcyI GRCGYC 2 cut(s) 538, 598
AfaI GTAC 5 cut(s) 232, 692, 870, 875, 1094
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 4 cut(s) 342, 728, 830, 946
AjnI CCWGG 2 cut(s) 493, 742
AluBI AGCT 9 cut(s) 325, 338, 466, 505, 608, 720, 796, 848, 1171
AluI AGCT 9 cut(s) 325, 338, 466, 505, 608, 720, 796, 848, 1171
Alw21I GWGCWC 3 cut(s) 47, 154, 1084
Alw26I GTCTC 3 cut(s) 194, 269, 865
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 2 cut(s) 734, 823
Ama87I CYCGRG 1 cut(s) 1166
ApaLI GTGCAC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 1190
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AseI ATTAAT 1 cut(s) 135
AspLEI GCGC 3 cut(s) 241, 600, 1144
AspS9I GGNCC 2 cut(s) 413, 1010
AvaI CYCGRG 1 cut(s) 1166
AvaII GGWCC 2 cut(s) 413, 1010
BaeGI GKGCMC 2 cut(s) 130, 154
BanI GGYRCC 1 cut(s) 597
BarI GAAGNNNNNNTAC 2 cut(s) 1041, 1073
BbsI GAAGAC 2 cut(s) 33, 877
Bbv12I GWGCWC 3 cut(s) 47, 154, 1084
BccI CCATC 1 cut(s) 1180
BceAI ACGGC 1 cut(s) 756
BciT130I CCWGG 2 cut(s) 495, 744
BclI TGATCA 1 cut(s) 54
BcoDI GTCTC 3 cut(s) 194, 269, 865
BfaI CTAG 5 cut(s) 225, 408, 549, 605, 1131
BfmI CTRYAG 1 cut(s) 684
BfoI RGCGCY 1 cut(s) 601
BfuAI ACCTGC 1 cut(s) 58
BisI GCNGC 1 cut(s) 838
BlsI GCNGC 1 cut(s) 839
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 2 cut(s) 495, 744
Bme18I GGWCC 2 cut(s) 413, 1010
BmeT110I CYCGRG 1 cut(s) 1166
BmgT120I GGNCC 2 cut(s) 413, 1010
BmiI GGNNCC 6 cut(s) 222, 288, 348, 571, 599, 1158
BmrFI CCNGG 2 cut(s) 495, 744
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 4 cut(s) 417, 521, 577, 672
BmuI ACTGGG 1 cut(s) 662
BpiI GAAGAC 2 cut(s) 33, 877
BplI GAGNNNNNCTC 2 cut(s) 1160, 1192
BpmI CTGGAG 1 cut(s) 305
BsaHI GRCGYC 2 cut(s) 538, 598
BsaJI CCNNGG 3 cut(s) 195, 494, 955
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 668, 1001
BseBI CCWGG 2 cut(s) 495, 744
BseDI CCNNGG 3 cut(s) 195, 494, 955
BseGI GGATG 3 cut(s) 663, 1109, 1178
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 4 cut(s) 486, 645, 833, 876
BseNI ACTGG 2 cut(s) 668, 1001
BseRI GAGGAG 2 cut(s) 178, 181
BseSI GKGCMC 2 cut(s) 130, 154
BshNI GGYRCC 1 cut(s) 597
BsiHKAI GWGCWC 3 cut(s) 47, 154, 1084
BsiHKCI CYCGRG 1 cut(s) 1166
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 3 cut(s) 194, 269, 865
BsmFI GGGAC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 1166
Bsp1286I GDGCHC 4 cut(s) 47, 130, 154, 1084
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 3 cut(s) 54, 739, 815
Bsp19I CCATGG 1 cut(s) 955
BspACI CCGC 1 cut(s) 838
BspCNI CTCAG 4 cut(s) 485, 646, 832, 875
BspLI GGNNCC 6 cut(s) 222, 288, 348, 571, 599, 1158
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 2 cut(s) 734, 823
BspT107I GGYRCC 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 690
BsrI ACTGG 2 cut(s) 668, 1001
BssECI CCNNGG 3 cut(s) 195, 494, 955
BssMI GATC 3 cut(s) 54, 739, 815
BssNI GRCGYC 2 cut(s) 538, 598
BssT1I CCWWGG 2 cut(s) 195, 955
Bst2UI CCWGG 2 cut(s) 495, 744
Bst4CI ACNGT 7 cut(s) 235, 417, 688, 873, 883, 982, 1062
BstACI GRCGYC 2 cut(s) 538, 598
BstAPI GCANNNNNTGC 2 cut(s) 125, 931
BstAUI TGTACA 1 cut(s) 690
BstC8I GCNNGC 3 cut(s) 906, 910, 1173
BstDEI CTNAG 6 cut(s) 291, 472, 654, 819, 833, 862
BstDSI CCRYGG 1 cut(s) 955
BstF5I GGATG 3 cut(s) 663, 1109, 1178
BstH2I RGCGCY 1 cut(s) 601
BstHHI GCGC 3 cut(s) 241, 600, 1144
BstKTI GATC 3 cut(s) 57, 742, 818
BstMAI GTCTC 3 cut(s) 194, 269, 865
BstMBI GATC 3 cut(s) 54, 739, 815
BstMWI GCNNNNNNNGC 4 cut(s) 125, 427, 931, 1148
BstNI CCWGG 2 cut(s) 495, 744
BstNSI RCATGY 2 cut(s) 488, 934
BstSCI CCNGG 2 cut(s) 493, 742
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 2 cut(s) 130, 154
BstV2I GAAGAC 2 cut(s) 33, 877
BstX2I RGATCY 1 cut(s) 815
BstYI RGATCY 1 cut(s) 815
BtgI CCRYGG 1 cut(s) 955
BtsCI GGATG 3 cut(s) 663, 1109, 1178
BtsIMutI CAGTG 5 cut(s) 578, 693, 809, 827, 962
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 906, 910, 1173
CfoI GCGC 3 cut(s) 241, 600, 1144
Cfr13I GGNCC 2 cut(s) 413, 1010
CseI GACGC 1 cut(s) 546
Csp6I GTAC 5 cut(s) 231, 691, 869, 874, 1093
CviAII CATG 7 cut(s) 271, 305, 397, 458, 485, 931, 956
CviQI GTAC 5 cut(s) 231, 691, 869, 874, 1093
DdeI CTNAG 6 cut(s) 291, 472, 654, 819, 833, 862
DinI GGCGCC 1 cut(s) 599
DpnI GATC 3 cut(s) 56, 741, 817
DpnII GATC 3 cut(s) 54, 739, 815
DrdI GACNNNNNNGTC 1 cut(s) 753
DseDI GACNNNNNNGTC 1 cut(s) 753
Eco130I CCWWGG 2 cut(s) 195, 955
Eco47I GGWCC 2 cut(s) 413, 1010
Eco57I CTGAAG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 1166
EcoRII CCWGG 2 cut(s) 493, 742
EcoT14I CCWWGG 2 cut(s) 195, 955
EcoT22I ATGCAT 1 cut(s) 514
EgeI GGCGCC 1 cut(s) 599
EheI GGCGCC 1 cut(s) 599
ErhI CCWWGG 2 cut(s) 195, 955
FaeI CATG 7 cut(s) 274, 308, 400, 461, 488, 934, 959
FaqI GGGAC 1 cut(s) 468
FatI CATG 7 cut(s) 270, 304, 396, 457, 484, 930, 955
FauNDI CATATG 1 cut(s) 1027
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 993
Fnu4HI GCNGC 1 cut(s) 838
FokI GGATG 3 cut(s) 650, 1096, 1165
Fsp4HI GCNGC 1 cut(s) 838
FspBI CTAG 5 cut(s) 225, 408, 549, 605, 1131
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 3 cut(s) 240, 599, 1143
GluI GCNGC 1 cut(s) 838
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 601
HapII CCGG 1 cut(s) 350
HgaI GACGC 1 cut(s) 546
HhaI GCGC 3 cut(s) 241, 600, 1144
Hin1I GRCGYC 2 cut(s) 538, 598
Hin1II CATG 7 cut(s) 274, 308, 400, 461, 488, 934, 959
Hin6I GCGC 3 cut(s) 239, 598, 1142
HinP1I GCGC 3 cut(s) 239, 598, 1142
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 1 cut(s) 728
HpaII CCGG 1 cut(s) 350
Hpy166II GTNNAC 4 cut(s) 152, 691, 962, 994
Hpy188I TCNGA 5 cut(s) 375, 655, 865, 978, 1102
Hpy188III TCNNGA 2 cut(s) 284, 785
Hpy8I GTNNAC 4 cut(s) 152, 691, 962, 994
Hpy99I CGWCG 2 cut(s) 540, 740
HpyAV CCTTC 2 cut(s) 611, 805
HpyCH4III ACNGT 7 cut(s) 235, 417, 688, 873, 883, 982, 1062
HpyCH4IV ACGT 1 cut(s) 748
HpyCH4V TGCA 7 cut(s) 67, 152, 484, 512, 590, 663, 1022
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 427, 931, 1148
HpyF3I CTNAG 6 cut(s) 291, 472, 654, 819, 833, 862
HpySE526I ACGT 1 cut(s) 748
Hsp92I GRCGYC 2 cut(s) 538, 598
Hsp92II CATG 7 cut(s) 274, 308, 400, 461, 488, 934, 959
HspAI GCGC 3 cut(s) 239, 598, 1142
KasI GGCGCC 1 cut(s) 597
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 3 cut(s) 54, 739, 815
LmnI GCTCC 4 cut(s) 220, 286, 1079, 1156
LweI GCATC 4 cut(s) 417, 521, 577, 672
MaeI CTAG 5 cut(s) 225, 408, 549, 605, 1131
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 3 cut(s) 764, 982, 1118
MalI GATC 3 cut(s) 56, 741, 817
MboI GATC 3 cut(s) 54, 739, 815
MboII GAAGA 3 cut(s) 38, 877, 1061
MflI RGATCY 1 cut(s) 815
MhlI GDGCHC 4 cut(s) 47, 130, 154, 1084
MluCI AATT 3 cut(s) 712, 1096, 1190
Mly113I GGCGCC 1 cut(s) 598
Mph1103I ATGCAT 1 cut(s) 514
MseI TTAA 2 cut(s) 135, 854
MslI CAYNNNNRTG 2 cut(s) 147, 401
MspA1I CMGCKG 1 cut(s) 796
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 495, 744
MvaI CCWGG 2 cut(s) 495, 744
MwoI GCNNNNNNNGC 4 cut(s) 125, 427, 931, 1148
NarI GGCGCC 1 cut(s) 598
NcoI CCATGG 1 cut(s) 955
NdeI CATATG 1 cut(s) 1027
NdeII GATC 3 cut(s) 54, 739, 815
NlaIII CATG 7 cut(s) 274, 308, 400, 461, 488, 934, 959
NlaIV GGNNCC 6 cut(s) 222, 288, 348, 571, 599, 1158
NmuCI GTSAC 1 cut(s) 982
NsbI TGCGCA 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 514
NspI RCATGY 2 cut(s) 488, 934
PaeR7I CTCGAG 1 cut(s) 1166
PcsI WCGNNNNNNNCGW 1 cut(s) 754
PfeI GAWTC 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 1 cut(s) 839
PluTI GGCGCC 1 cut(s) 601
PshBI ATTAAT 1 cut(s) 135
Psp6I CCWGG 2 cut(s) 493, 742
PspGI CCWGG 2 cut(s) 493, 742
PspN4I GGNNCC 6 cut(s) 222, 288, 348, 571, 599, 1158
PspPI GGNCC 2 cut(s) 413, 1010
PspXI VCTCGAGB 1 cut(s) 1166
PsuI RGATCY 1 cut(s) 815
PvuII CAGCTG 1 cut(s) 796
RsaI GTAC 5 cut(s) 232, 692, 870, 875, 1094
RsaNI GTAC 5 cut(s) 231, 691, 869, 874, 1093
RseI CAYNNNNRTG 2 cut(s) 147, 401
SaqAI TTAA 2 cut(s) 135, 854
SatI GCNGC 1 cut(s) 838
Sau3AI GATC 3 cut(s) 54, 739, 815
Sau96I GGNCC 2 cut(s) 413, 1010
ScaI AGTACT 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 495, 744
SduI GDGCHC 4 cut(s) 47, 130, 154, 1084
SfaNI GCATC 4 cut(s) 417, 521, 577, 672
SfcI CTRYAG 1 cut(s) 684
SfoI GGCGCC 1 cut(s) 599
Sfr274I CTCGAG 1 cut(s) 1166
SinI GGWCC 2 cut(s) 413, 1010
SlaI CTCGAG 1 cut(s) 1166
SmiMI CAYNNNNRTG 2 cut(s) 147, 401
SmlI CTYRAG 1 cut(s) 1166
SmoI CTYRAG 1 cut(s) 1166
Sse9I AATT 3 cut(s) 712, 1096, 1190
SsiI CCGC 1 cut(s) 838
SspDI GGCGCC 1 cut(s) 597
SspI AATATT 2 cut(s) 896, 1042
SspMI CTAG 5 cut(s) 225, 408, 549, 605, 1131
StyD4I CCNGG 2 cut(s) 493, 742
StyI CCWWGG 2 cut(s) 195, 955
TaaI ACNGT 7 cut(s) 235, 417, 688, 873, 883, 982, 1062
TaiI ACGT 1 cut(s) 751
TaqI TCGA 2 cut(s) 535, 1167
TasI AATT 3 cut(s) 712, 1096, 1190
TatI WGTACW 4 cut(s) 690, 868, 873, 1092
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 2 cut(s) 135, 854
Tru9I TTAA 2 cut(s) 135, 854
TscAI CASTG 5 cut(s) 585, 693, 809, 827, 969
TseFI GTSAC 1 cut(s) 982
Tsp45I GTSAC 1 cut(s) 982
TspDTI ATGAA 1 cut(s) 385
TspGWI ACGGA 2 cut(s) 329, 1171
TspRI CASTG 5 cut(s) 585, 693, 809, 827, 969
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 413, 1010
VspI ATTAAT 1 cut(s) 135
XapI RAATTY 1 cut(s) 1190
XceI RCATGY 2 cut(s) 488, 934
XhoI CTCGAG 1 cut(s) 1166
XmiI GTMKAC 1 cut(s) 993
XspI CTAG 5 cut(s) 225, 408, 549, 605, 1131
ZrmI AGTACT 1 cut(s) 875
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.