MD08G1246800.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012465
Physical Location & Seq
Reverse (-)
31069871 .. 31072757
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1246800.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGCAAGAGGCAGAGAAGACTATGAGAAGCACCCGGGTTTGCTCAAACTCTTGCAGATTCTCTCGTTCTTGGTAGTTTTTGTTGTGGGGATTGTTATAGGATTAGCAACGAGCGCCCATATTAACCGGCATTTTGGTTCACAAACTGAGATTTATTCGTTTATCAATCACTTCTCCGCCAATAAGCCGCACAAGGAGAAGAATTGTACTACTGTGCAAGTTAGTGACAAGGTTGATTGTATAAGCATGGAGACGTTTCTTCAGCCGAAGAATTTGACGCACGGTCTGTCTGATGATGAGCTCTTTTGGAGAGCTTCAATGGTGCCGCAGAAGCCAGAGTTTCCATTCGAGAGAGGGCCTAAAGTGGCTTTCATGTTTTTGACTAGAGGACCTTTGCCCTTGTTGCCATTGTGGGAGAGGTTCTTCCATGGCCATGACAAGTATTTCTCAATTTATGTCCATGTAATTCCGGGTTATGAGCTCAATGTATCTACCACCTCTCCTTTTTATCAACGCCAAATCCCCAGTCAGCCTGTTTCATGGGGGACAGTAACATTGGTTGATGCAGAGAGGCGGCTTCTAGCGAATGCCTTGCTTGACTTCTCAAATGAGCGCTTTGTTCTTCTCTCTGAGAGCTGCATCCCGATCTATAATTTCCCTACTGTCTACAAGTATCTGATTGGTTCAAACCATAGCTATGTTGAGTCATACGATAATCCTGGGCGTTATGGGCGTGGGCGTTACAGCCGCGAAATGTTTCCTGATATTCAACTGTATCAGTGGAGGAAAGGGTCTCAGTGGTTTGAACTTAGCCGTGCGTTATCCGTGTATTTAGTGTCGGAAACAAAGTATTACACAATCTTCAGCAAATATTGTTTGCCTGCATGCTACCCGGATGAGCATTACCTGCCTACTTACTTCAACATGTTCCATGGATGGCTGAATTCGAATAGGACTGTGACGTGGGTAGACTGGTCACTGGGAGGTCCGCACCCTGCAACGTACGGAGGAGACAATATTACGGAAGATTTCGTACGGTCTATAAGGAACAATGGAGCGCTGTGTCAGTACAATGCGGAGATGACATCCATATGTTACCTATTTGCTCGGAAGTTTGCACCAACTGCATTGGGGCCTCTGCTCGAGTTCGCCGCATCGGTGATGGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

391

Amino Acids

45.09

Weight (kDa)

7.2

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 122 - 349 3.1e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 322
AccI GTMKAC 2 cut(s) 666, 969
AccII CGCG 1 cut(s) 750
AciI CCGC 8 cut(s) 177, 188, 326, 574, 748, 989, 1076, 1152
AcoI YGGCCR 1 cut(s) 430
AcsI RAATTY 2 cut(s) 271, 943
AcuI CTGAAG 2 cut(s) 245, 847
AfaI GTAC 4 cut(s) 208, 1004, 1035, 1070
AfeI AGCGCT 2 cut(s) 614, 1059
AflIII ACRYGT 1 cut(s) 924
AgsI TTSAA 5 cut(s) 318, 687, 770, 806, 922
AhdI GACNNNNNGTC 1 cut(s) 282
AjiI CACGTC 1 cut(s) 963
AjnI CCWGG 1 cut(s) 718
AluBI AGCT 5 cut(s) 301, 314, 481, 636, 696
AluI AGCT 5 cut(s) 301, 314, 481, 636, 696
Alw21I GWGCWC 2 cut(s) 303, 483
Alw26I GTCTC 3 cut(s) 245, 798, 1005
Ama87I CYCGRG 2 cut(s) 34, 1142
Aor51HI AGCGCT 2 cut(s) 614, 1059
AoxI GGCC 3 cut(s) 356, 430, 1133
ApeKI GCWGC 1 cut(s) 636
ApoI RAATTY 2 cut(s) 271, 943
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 3 cut(s) 116, 615, 1060
AspS9I GGNCC 4 cut(s) 356, 389, 986, 1133
AsuC2I CCSGG 4 cut(s) 35, 36, 471, 893
AsuHPI GGTGA 1 cut(s) 1171
AsuII TTCGAA 1 cut(s) 947
AvaI CYCGRG 2 cut(s) 34, 1142
AvaII GGWCC 2 cut(s) 389, 986
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 303, 483
BarI GAAGNNNNNNTAC 2 cut(s) 1017, 1049
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 303, 483
BbvI GCAGC 1 cut(s) 623
BccI CCATC 2 cut(s) 930, 1156
BceAI ACGGC 1 cut(s) 798
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BciT130I CCWGG 1 cut(s) 720
BcnI CCSGG 4 cut(s) 35, 36, 471, 893
BcoDI GTCTC 3 cut(s) 245, 798, 1005
BfaI CTAG 2 cut(s) 384, 581
BfoI RGCGCY 3 cut(s) 117, 616, 1061
BfuAI ACCTGC 1 cut(s) 915
BisI GCNGC 6 cut(s) 188, 326, 575, 637, 748, 1152
BlsI GCNGC 6 cut(s) 189, 327, 576, 638, 749, 1153
Bme1390I CCNGG 5 cut(s) 35, 36, 471, 720, 893
Bme18I GGWCC 2 cut(s) 389, 986
BmeRI GACNNNNNGTC 1 cut(s) 282
BmeT110I CYCGRG 2 cut(s) 34, 1142
BmgBI CACGTC 1 cut(s) 963
BmgT120I GGNCC 4 cut(s) 356, 389, 986, 1133
BmiI GGNNCC 2 cut(s) 324, 1134
BmrFI CCNGG 5 cut(s) 35, 36, 471, 720, 893
BmrI ACTGGG 2 cut(s) 519, 989
BmsI GCATC 3 cut(s) 553, 648, 1163
BmuI ACTGGG 2 cut(s) 519, 989
BpiI GAAGAC 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 947
BpuMI CCSGG 4 cut(s) 35, 36, 471, 893
BsaI GGTCTC 1 cut(s) 798
BsaJI CCNNGG 4 cut(s) 34, 427, 719, 931
BsaXI ACNNNNNCTCC 2 cut(s) 484, 514
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 3 cut(s) 525, 977, 984
BseBI CCWGG 1 cut(s) 720
BseDI CCNNGG 4 cut(s) 34, 427, 719, 931
BseGI GGATG 4 cut(s) 639, 901, 941, 1085
BseMII CTCAG 3 cut(s) 138, 621, 809
BseNI ACTGG 3 cut(s) 525, 977, 984
BseRI GAGGAG 1 cut(s) 1023
BseXI GCAGC 1 cut(s) 623
Bsh1236I CGCG 1 cut(s) 750
BshFI GGCC 3 cut(s) 358, 432, 1135
BshNI GGYRCC 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 303, 483
BsiHKCI CYCGRG 2 cut(s) 34, 1142
BsiSI CCGG 4 cut(s) 35, 127, 470, 893
BsiWI CGTACG 2 cut(s) 1002, 1033
BslFI GGGAC 1 cut(s) 559
BsmAI GTCTC 3 cut(s) 245, 798, 1005
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 358, 432, 1135
Bso31I GGTCTC 1 cut(s) 798
BsoBI CYCGRG 2 cut(s) 34, 1142
Bsp119I TTCGAA 1 cut(s) 947
Bsp1286I GDGCHC 2 cut(s) 303, 483
Bsp143I GATC 1 cut(s) 645
Bsp19I CCATGG 2 cut(s) 427, 931
BspACI CCGC 8 cut(s) 177, 188, 326, 574, 748, 989, 1076, 1152
BspANI GGCC 3 cut(s) 358, 432, 1135
BspCNI CTCAG 3 cut(s) 139, 622, 808
BspFNI CGCG 1 cut(s) 750
BspLI GGNNCC 2 cut(s) 324, 1134
BspMI ACCTGC 1 cut(s) 915
BspT104I TTCGAA 1 cut(s) 947
BspT107I GGYRCC 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 798
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 3 cut(s) 525, 977, 984
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 4 cut(s) 34, 427, 719, 931
BssMI GATC 1 cut(s) 645
BssT1I CCWWGG 2 cut(s) 427, 931
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 7 cut(s) 214, 284, 550, 664, 774, 958, 1038
BstAPI GCANNNNNTGC 2 cut(s) 907, 1124
BstBI TTCGAA 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 882, 886
BstDEI CTNAG 4 cut(s) 147, 630, 795, 809
BstDSI CCRYGG 2 cut(s) 427, 931
BstF5I GGATG 4 cut(s) 639, 901, 941, 1085
BstFNI CGCG 1 cut(s) 750
BstH2I RGCGCY 3 cut(s) 117, 616, 1061
BstHHI GCGC 3 cut(s) 116, 615, 1060
BstKTI GATC 1 cut(s) 648
BstMAI GTCTC 3 cut(s) 245, 798, 1005
BstMBI GATC 1 cut(s) 645
BstMWI GCNNNNNNNGC 6 cut(s) 113, 331, 403, 730, 907, 1124
BstNI CCWGG 1 cut(s) 720
BstNSI RCATGY 2 cut(s) 888, 928
BstSCI CCNGG 5 cut(s) 33, 34, 469, 718, 891
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 1 cut(s) 623
BstV2I GAAGAC 1 cut(s) 24
BsuRI GGCC 3 cut(s) 358, 432, 1135
BtgI CCRYGG 2 cut(s) 427, 931
BtrI CACGTC 1 cut(s) 963
BtsCI GGATG 4 cut(s) 639, 901, 941, 1085
BtsIMutI CAGTG 3 cut(s) 785, 803, 977
BveI ACCTGC 1 cut(s) 915
Cac8I GCNNGC 2 cut(s) 882, 886
CfoI GCGC 3 cut(s) 116, 615, 1060
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 4 cut(s) 356, 389, 986, 1133
Cfr9I CCCGGG 1 cut(s) 34
CseI GACGC 1 cut(s) 286
Csp6I GTAC 4 cut(s) 207, 1003, 1034, 1069
CviAII CATG 9 cut(s) 247, 373, 428, 434, 461, 540, 885, 925, 932
CviQI GTAC 4 cut(s) 207, 1003, 1034, 1069
DdeI CTNAG 4 cut(s) 147, 630, 795, 809
DpnI GATC 1 cut(s) 647
DpnII GATC 1 cut(s) 645
DriI GACNNNNNGTC 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 430
Eam1105I GACNNNNNGTC 1 cut(s) 282
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 301, 481
Eco130I CCWWGG 2 cut(s) 427, 931
Eco24I GRGCYC 2 cut(s) 303, 483
Eco31I GGTCTC 1 cut(s) 798
Eco47I GGWCC 2 cut(s) 389, 986
Eco47III AGCGCT 2 cut(s) 614, 1059
Eco53kI GAGCTC 2 cut(s) 301, 481
Eco57I CTGAAG 2 cut(s) 245, 847
Eco88I CYCGRG 2 cut(s) 34, 1142
EcoICRI GAGCTC 2 cut(s) 301, 481
EcoO109I RGGNCCY 3 cut(s) 356, 389, 1133
EcoRI GAATTC 1 cut(s) 943
EcoRII CCWGG 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 427, 931
EcoT38I GRGCYC 2 cut(s) 303, 483
ErhI CCWWGG 2 cut(s) 427, 931
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 9 cut(s) 250, 376, 431, 437, 464, 543, 888, 928, 935
FaqI GGGAC 1 cut(s) 559
FatI CATG 9 cut(s) 246, 372, 427, 433, 460, 539, 884, 924, 931
FauNDI CATATG 1 cut(s) 1091
FblI GTMKAC 2 cut(s) 666, 969
Fnu4HI GCNGC 6 cut(s) 188, 326, 575, 637, 748, 1152
FokI GGATG 4 cut(s) 626, 908, 948, 1072
FriOI GRGCYC 2 cut(s) 303, 483
Fsp4HI GCNGC 6 cut(s) 188, 326, 575, 637, 748, 1152
FspBI CTAG 2 cut(s) 384, 581
GlaI GCGC 3 cut(s) 115, 614, 1059
GluI GCNGC 6 cut(s) 188, 326, 575, 637, 748, 1152
HaeII RGCGCY 3 cut(s) 117, 616, 1061
HaeIII GGCC 3 cut(s) 358, 432, 1135
HapII CCGG 4 cut(s) 35, 127, 470, 893
HgaI GACGC 1 cut(s) 286
HhaI GCGC 3 cut(s) 116, 615, 1060
Hin1II CATG 9 cut(s) 250, 376, 431, 437, 464, 543, 888, 928, 935
Hin6I GCGC 3 cut(s) 114, 613, 1058
HinP1I GCGC 3 cut(s) 114, 613, 1058
HinfI GANTC 2 cut(s) 58, 704
HpaII CCGG 4 cut(s) 35, 127, 470, 893
HphI GGTGA 1 cut(s) 1171
Hpy166II GTNNAC 3 cut(s) 140, 667, 970
Hpy188I TCNGA 5 cut(s) 292, 631, 678, 841, 1110
Hpy188III TCNNGA 3 cut(s) 349, 643, 761
Hpy8I GTNNAC 3 cut(s) 140, 667, 970
HpyCH4III ACNGT 7 cut(s) 214, 284, 550, 664, 774, 958, 1038
HpyCH4IV ACGT 3 cut(s) 254, 962, 1001
HpyCH4V TGCA 8 cut(s) 55, 217, 566, 639, 884, 998, 1118, 1127
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 331, 403, 730, 907, 1124
HpyF3I CTNAG 4 cut(s) 147, 630, 795, 809
HpySE526I ACGT 3 cut(s) 254, 962, 1001
Hsp92II CATG 9 cut(s) 250, 376, 431, 437, 464, 543, 888, 928, 935
HspAI GCGC 3 cut(s) 114, 613, 1058
Kzo9I GATC 1 cut(s) 645
LmnI GCTCC 1 cut(s) 1055
Lsp1109I GCAGC 1 cut(s) 623
LweI GCATC 3 cut(s) 553, 648, 1163
MaeI CTAG 2 cut(s) 384, 581
MaeII ACGT 3 cut(s) 254, 962, 1001
MaeIII GTNAC 6 cut(s) 224, 550, 740, 958, 975, 1094
MalI GATC 1 cut(s) 647
MboI GATC 1 cut(s) 645
MboII GAAGA 8 cut(s) 29, 211, 251, 280, 415, 614, 853, 1037
MhlI GDGCHC 2 cut(s) 303, 483
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 6 cut(s) 202, 271, 450, 465, 652, 943
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 819
MnlI CCTC 9 cut(s) 347, 380, 411, 508, 564, 777, 977, 1001, 1146
Mox20I TGGCCA 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 756
MscI TGGCCA 1 cut(s) 432
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 3 cut(s) 432, 696, 1090
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 4 cut(s) 35, 127, 470, 893
MspR9I CCNGG 5 cut(s) 35, 36, 471, 720, 893
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 720
MvnI CGCG 1 cut(s) 750
MwoI GCNNNNNNNGC 6 cut(s) 113, 331, 403, 730, 907, 1124
NciI CCSGG 4 cut(s) 35, 36, 471, 893
NcoI CCATGG 2 cut(s) 427, 931
NdeI CATATG 1 cut(s) 1091
NdeII GATC 1 cut(s) 645
NlaIII CATG 9 cut(s) 250, 376, 431, 437, 464, 543, 888, 928, 935
NlaIV GGNNCC 2 cut(s) 324, 1134
NmuCI GTSAC 3 cut(s) 224, 958, 975
NspI RCATGY 2 cut(s) 888, 928
NspV TTCGAA 1 cut(s) 947
PaeI GCATGC 1 cut(s) 888
PaeR7I CTCGAG 1 cut(s) 1142
PciI ACATGT 1 cut(s) 924
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 1 cut(s) 58
Pfl23II CGTACG 2 cut(s) 1002, 1033
PkrI GCNGC 6 cut(s) 189, 327, 576, 638, 749, 1153
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PpuMI RGGWCCY 1 cut(s) 389
PscI ACATGT 1 cut(s) 924
Psp124BI GAGCTC 2 cut(s) 303, 483
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 718
PspGI CCWGG 1 cut(s) 718
PspLI CGTACG 2 cut(s) 1002, 1033
PspN4I GGNNCC 2 cut(s) 324, 1134
PspPI GGNCC 4 cut(s) 356, 389, 986, 1133
PspPPI RGGWCCY 1 cut(s) 389
PspXI VCTCGAGB 1 cut(s) 1142
RsaI GTAC 4 cut(s) 208, 1004, 1035, 1070
RsaNI GTAC 4 cut(s) 207, 1003, 1034, 1069
RseI CAYNNNNRTG 3 cut(s) 432, 696, 1090
SacI GAGCTC 2 cut(s) 303, 483
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 6 cut(s) 188, 326, 575, 637, 748, 1152
Sau3AI GATC 1 cut(s) 645
Sau96I GGNCC 4 cut(s) 356, 389, 986, 1133
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 5 cut(s) 35, 36, 471, 720, 893
SduI GDGCHC 2 cut(s) 303, 483
SfaNI GCATC 3 cut(s) 553, 648, 1163
Sfr274I CTCGAG 1 cut(s) 1142
SfuI TTCGAA 1 cut(s) 947
SinI GGWCC 2 cut(s) 389, 986
SlaI CTCGAG 1 cut(s) 1142
SmaI CCCGGG 1 cut(s) 36
SmiMI CAYNNNNRTG 3 cut(s) 432, 696, 1090
SmlI CTYRAG 1 cut(s) 1142
SmoI CTYRAG 1 cut(s) 1142
SphI GCATGC 1 cut(s) 888
Sse9I AATT 6 cut(s) 202, 271, 450, 465, 652, 943
SsiI CCGC 8 cut(s) 177, 188, 326, 574, 748, 989, 1076, 1152
SspI AATATT 3 cut(s) 872, 1018, 1169
SspMI CTAG 2 cut(s) 384, 581
SstI GAGCTC 2 cut(s) 303, 483
StyD4I CCNGG 5 cut(s) 33, 34, 469, 718, 891
StyI CCWWGG 2 cut(s) 427, 931
TaaI ACNGT 7 cut(s) 214, 284, 550, 664, 774, 958, 1038
TaiI ACGT 3 cut(s) 257, 965, 1004
TaqI TCGA 3 cut(s) 348, 947, 1143
TasI AATT 6 cut(s) 202, 271, 450, 465, 652, 943
TatI WGTACW 2 cut(s) 206, 1068
TauI GCSGC 5 cut(s) 190, 328, 577, 750, 1154
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 3 cut(s) 785, 803, 984
TseFI GTSAC 3 cut(s) 224, 958, 975
TseI GCWGC 1 cut(s) 636
Tsp45I GTSAC 3 cut(s) 224, 958, 975
TspDTI ATGAA 2 cut(s) 361, 528
TspGWI ACGGA 3 cut(s) 814, 1020, 1037
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 3 cut(s) 785, 803, 984
VpaK11BI GGWCC 2 cut(s) 389, 986
XapI RAATTY 2 cut(s) 271, 943
XceI RCATGY 2 cut(s) 888, 928
XhoI CTCGAG 1 cut(s) 1142
XmaI CCCGGG 1 cut(s) 34
XmiI GTMKAC 2 cut(s) 666, 969
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 2 cut(s) 384, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.