pycom08g21390

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012465
Physical Location & Seq
Reverse (-)
20879222 .. 20881337
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21390.2

Sequence Viewer

Length: 927 bp
ATGGAGACGTTTCTTCAGCCGAAGAATTTGACGCACGGTCTGTCTGATGATGAGCTCTTTTGGAGAGCTTCAATGGTGCCGCAGAAGCCAGAGTTTCCATTCGAGAGAGTGCCTAAAGTGGCTTTCATGTTTTTGACTAGAGGACCTTTGCCCTTGTTGCCATTGTGGGAGAGGTTCTTCCATGGCCATGACAAGTATTTCTCAATTTACGTCCATGTAATTCCGGGTTATGAGCTCAATGTATCTACCACCTCTCCTTTTTATCAACGCCAAATCCCCAGTCAGCCTGTTTCATGGGGGACAGTAACATTGGTTGATGCAGAGAGGCGGCTTCTAGCGAATGCCTTGCTTGACTTCTCAAATGAGCGCTTTGTTCTTCTCTCTGAGAGCTGCATCCCGATCTATAATTTCCCCACTGTCTACAAGTATCTCATCGGTTCAAACCATAGCTATGTTGAGTCATACGATAATCCTGGGCGTTACGGGCGTGGGCGTTACAGCCGCGAAATGTTTCCTGATATTCAACTGTATCAGTGGAGGAAAGGGTCTCAGTGGTTTGAACTTAGCCGTGCGTTATCCGTGTATATAGTGTCGGAAACAAAGTATTACACAATCTTCAGCAAATATTGTTTGCCTGCATGCTACCCGGATGAGCATTACCTGCCTACTTACTTCAACATGTTCCATGGATGGCTGAATTCGAATAGGACTGTGACGTGGGTAGACTGGTCACTGGGAGGTCCGCACCCTGCAACGTACGGAGGAGACAATATTACGGAAGATTTCGTACGGTCTATAAGGAACAATGGAGCGCTGTGTCAGTACAATGCGGAGATGACATCCATATGTTACCTATTTGCTCGGAAGTTTGCACCAACTGCACTGGGGCCTCTTCTCGAGTTCGCCGCGTCGGTGATGGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

309

Amino Acids

35.95

Weight (kDa)

6.45

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 76
AccI GTMKAC 2 cut(s) 420, 723
AccII CGCG 2 cut(s) 504, 908
AciI CCGC 6 cut(s) 80, 328, 502, 743, 830, 906
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 2 cut(s) 25, 697
AcuI CTGAAG 1 cut(s) 601
AfaI GTAC 3 cut(s) 758, 789, 824
AfeI AGCGCT 2 cut(s) 368, 813
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 5 cut(s) 72, 441, 524, 560, 676
AhdI GACNNNNNGTC 1 cut(s) 36
AjiI CACGTC 1 cut(s) 717
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 5 cut(s) 55, 68, 235, 390, 450
AluI AGCT 5 cut(s) 55, 68, 235, 390, 450
Alw21I GWGCWC 2 cut(s) 57, 237
Alw26I GTCTC 2 cut(s) 552, 759
Ama87I CYCGRG 1 cut(s) 896
Aor51HI AGCGCT 2 cut(s) 368, 813
AoxI GGCC 2 cut(s) 184, 887
ApeKI GCWGC 1 cut(s) 390
ApoI RAATTY 2 cut(s) 25, 697
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 369, 814
AspS9I GGNCC 3 cut(s) 143, 740, 887
AsuC2I CCSGG 2 cut(s) 225, 647
AsuHPI GGTGA 1 cut(s) 925
AsuII TTCGAA 1 cut(s) 701
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 2 cut(s) 143, 740
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 57, 237
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
Bbv12I GWGCWC 2 cut(s) 57, 237
BbvI GCAGC 1 cut(s) 377
BccI CCATC 2 cut(s) 684, 910
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 2 cut(s) 225, 647
BcoDI GTCTC 2 cut(s) 552, 759
BfaI CTAG 2 cut(s) 138, 335
BfoI RGCGCY 2 cut(s) 370, 815
BfuAI ACCTGC 1 cut(s) 669
BisI GCNGC 5 cut(s) 80, 329, 391, 502, 906
BlsI GCNGC 5 cut(s) 81, 330, 392, 503, 907
Bme1390I CCNGG 3 cut(s) 225, 474, 647
Bme18I GGWCC 2 cut(s) 143, 740
BmeRI GACNNNNNGTC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 896
BmgBI CACGTC 1 cut(s) 717
BmgT120I GGNCC 3 cut(s) 143, 740, 887
BmiI GGNNCC 2 cut(s) 78, 888
BmrFI CCNGG 3 cut(s) 225, 474, 647
BmrI ACTGGG 3 cut(s) 273, 743, 893
BmsI GCATC 2 cut(s) 307, 402
BmuI ACTGGG 3 cut(s) 273, 743, 893
Bpu14I TTCGAA 1 cut(s) 701
BpuMI CCSGG 2 cut(s) 225, 647
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 3 cut(s) 181, 473, 685
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bse1I ACTGG 4 cut(s) 279, 731, 738, 888
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 3 cut(s) 181, 473, 685
BseGI GGATG 4 cut(s) 393, 655, 695, 839
BseMII CTCAG 2 cut(s) 375, 563
BseNI ACTGG 4 cut(s) 279, 731, 738, 888
BseRI GAGGAG 1 cut(s) 777
BseXI GCAGC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 2 cut(s) 504, 908
BshFI GGCC 2 cut(s) 186, 889
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 57, 237
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 2 cut(s) 224, 647
BsiWI CGTACG 2 cut(s) 756, 787
BslFI GGGAC 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 552, 759
BsmFI GGGAC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 186, 889
Bso31I GGTCTC 1 cut(s) 552
BsoBI CYCGRG 1 cut(s) 896
Bsp119I TTCGAA 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 57, 237
Bsp143I GATC 1 cut(s) 399
Bsp19I CCATGG 2 cut(s) 181, 685
BspACI CCGC 6 cut(s) 80, 328, 502, 743, 830, 906
BspANI GGCC 2 cut(s) 186, 889
BspCNI CTCAG 2 cut(s) 376, 562
BspFNI CGCG 2 cut(s) 504, 908
BspLI GGNNCC 2 cut(s) 78, 888
BspMI ACCTGC 1 cut(s) 669
BspT104I TTCGAA 1 cut(s) 701
BspT107I GGYRCC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 552
BsrI ACTGG 4 cut(s) 279, 731, 738, 888
BssECI CCNNGG 3 cut(s) 181, 473, 685
BssMI GATC 1 cut(s) 399
BssT1I CCWWGG 2 cut(s) 181, 685
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 6 cut(s) 38, 304, 418, 528, 712, 792
Bst6I CTCTTC 1 cut(s) 897
BstAPI GCANNNNNTGC 2 cut(s) 661, 878
BstBI TTCGAA 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 636, 640
BstDEI CTNAG 3 cut(s) 384, 549, 563
BstDSI CCRYGG 2 cut(s) 181, 685
BstF5I GGATG 4 cut(s) 393, 655, 695, 839
BstFNI CGCG 2 cut(s) 504, 908
BstH2I RGCGCY 2 cut(s) 370, 815
BstHHI GCGC 2 cut(s) 369, 814
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 2 cut(s) 552, 759
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 5 cut(s) 85, 157, 484, 661, 878
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 642, 682
BstSCI CCNGG 3 cut(s) 223, 472, 645
BstUI CGCG 2 cut(s) 504, 908
BstV1I GCAGC 1 cut(s) 377
BsuRI GGCC 2 cut(s) 186, 889
BtgI CCRYGG 2 cut(s) 181, 685
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 4 cut(s) 393, 655, 695, 839
BtsIMutI CAGTG 5 cut(s) 414, 539, 557, 731, 881
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 636, 640
CfoI GCGC 2 cut(s) 369, 814
Cfr13I GGNCC 3 cut(s) 143, 740, 887
CseI GACGC 2 cut(s) 40, 897
Csp6I GTAC 3 cut(s) 757, 788, 823
CviAII CATG 8 cut(s) 127, 182, 188, 215, 294, 639, 679, 686
CviQI GTAC 3 cut(s) 757, 788, 823
DdeI CTNAG 3 cut(s) 384, 549, 563
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
DriI GACNNNNNGTC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 897
Eam1105I GACNNNNNGTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 897
Ecl136II GAGCTC 2 cut(s) 55, 235
Eco130I CCWWGG 2 cut(s) 181, 685
Eco24I GRGCYC 2 cut(s) 57, 237
Eco31I GGTCTC 1 cut(s) 552
Eco47I GGWCC 2 cut(s) 143, 740
Eco47III AGCGCT 2 cut(s) 368, 813
Eco53kI GAGCTC 2 cut(s) 55, 235
Eco57I CTGAAG 1 cut(s) 601
Eco88I CYCGRG 1 cut(s) 896
EcoICRI GAGCTC 2 cut(s) 55, 235
EcoO109I RGGNCCY 2 cut(s) 143, 887
EcoRI GAATTC 1 cut(s) 697
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 181, 685
EcoT38I GRGCYC 2 cut(s) 57, 237
ErhI CCWWGG 2 cut(s) 181, 685
FaeI CATG 8 cut(s) 130, 185, 191, 218, 297, 642, 682, 689
FaqI GGGAC 1 cut(s) 313
FatI CATG 8 cut(s) 126, 181, 187, 214, 293, 638, 678, 685
FauNDI CATATG 1 cut(s) 845
FblI GTMKAC 2 cut(s) 420, 723
Fnu4HI GCNGC 5 cut(s) 80, 329, 391, 502, 906
FokI GGATG 4 cut(s) 380, 662, 702, 826
FriOI GRGCYC 2 cut(s) 57, 237
Fsp4HI GCNGC 5 cut(s) 80, 329, 391, 502, 906
FspBI CTAG 2 cut(s) 138, 335
GlaI GCGC 2 cut(s) 368, 813
GluI GCNGC 5 cut(s) 80, 329, 391, 502, 906
HaeII RGCGCY 2 cut(s) 370, 815
HaeIII GGCC 2 cut(s) 186, 889
HapII CCGG 2 cut(s) 224, 647
HgaI GACGC 2 cut(s) 40, 897
HhaI GCGC 2 cut(s) 369, 814
Hin1II CATG 8 cut(s) 130, 185, 191, 218, 297, 642, 682, 689
Hin6I GCGC 2 cut(s) 367, 812
HinP1I GCGC 2 cut(s) 367, 812
HinfI GANTC 1 cut(s) 458
HpaII CCGG 2 cut(s) 224, 647
HphI GGTGA 1 cut(s) 925
Hpy166II GTNNAC 2 cut(s) 421, 724
Hpy188I TCNGA 4 cut(s) 46, 385, 595, 864
Hpy188III TCNNGA 4 cut(s) 103, 397, 515, 896
Hpy8I GTNNAC 2 cut(s) 421, 724
Hpy99I CGWCG 1 cut(s) 913
HpyCH4III ACNGT 6 cut(s) 38, 304, 418, 528, 712, 792
HpyCH4IV ACGT 4 cut(s) 8, 210, 716, 755
HpyCH4V TGCA 6 cut(s) 320, 393, 638, 752, 872, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 157, 484, 661, 878
HpyF3I CTNAG 3 cut(s) 384, 549, 563
HpySE526I ACGT 4 cut(s) 8, 210, 716, 755
Hsp92II CATG 8 cut(s) 130, 185, 191, 218, 297, 642, 682, 689
HspAI GCGC 2 cut(s) 367, 812
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 809
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 2 cut(s) 307, 402
MaeI CTAG 2 cut(s) 138, 335
MaeII ACGT 4 cut(s) 8, 210, 716, 755
MaeIII GTNAC 6 cut(s) 304, 479, 494, 712, 729, 848
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MboII GAAGA 7 cut(s) 5, 34, 169, 368, 607, 791, 884
MhlI GDGCHC 2 cut(s) 57, 237
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 5 cut(s) 25, 204, 219, 406, 697
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 573
MnlI CCTC 8 cut(s) 134, 165, 262, 318, 531, 731, 755, 900
Mox20I TGGCCA 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 1 cut(s) 186
MslI CAYNNNNRTG 3 cut(s) 186, 450, 844
Msp20I TGGCCA 1 cut(s) 186
MspI CCGG 2 cut(s) 224, 647
MspR9I CCNGG 3 cut(s) 225, 474, 647
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 2 cut(s) 504, 908
MwoI GCNNNNNNNGC 5 cut(s) 85, 157, 484, 661, 878
NciI CCSGG 2 cut(s) 225, 647
NcoI CCATGG 2 cut(s) 181, 685
NdeI CATATG 1 cut(s) 845
NdeII GATC 1 cut(s) 399
NlaIII CATG 8 cut(s) 130, 185, 191, 218, 297, 642, 682, 689
NlaIV GGNNCC 2 cut(s) 78, 888
NmuCI GTSAC 2 cut(s) 712, 729
NspI RCATGY 2 cut(s) 642, 682
NspV TTCGAA 1 cut(s) 701
PaeI GCATGC 1 cut(s) 642
PaeR7I CTCGAG 1 cut(s) 896
PciI ACATGT 1 cut(s) 678
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 510
Pfl23II CGTACG 2 cut(s) 756, 787
PkrI GCNGC 5 cut(s) 81, 330, 392, 503, 907
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 2 cut(s) 57, 237
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspLI CGTACG 2 cut(s) 756, 787
PspN4I GGNNCC 2 cut(s) 78, 888
PspPI GGNCC 3 cut(s) 143, 740, 887
PspPPI RGGWCCY 1 cut(s) 143
RsaI GTAC 3 cut(s) 758, 789, 824
RsaNI GTAC 3 cut(s) 757, 788, 823
RseI CAYNNNNRTG 3 cut(s) 186, 450, 844
SacI GAGCTC 2 cut(s) 57, 237
SatI GCNGC 5 cut(s) 80, 329, 391, 502, 906
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 3 cut(s) 143, 740, 887
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 3 cut(s) 225, 474, 647
SduI GDGCHC 2 cut(s) 57, 237
SfaNI GCATC 2 cut(s) 307, 402
Sfr274I CTCGAG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 701
SinI GGWCC 2 cut(s) 143, 740
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 3 cut(s) 186, 450, 844
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SphI GCATGC 1 cut(s) 642
Sse9I AATT 5 cut(s) 25, 204, 219, 406, 697
SsiI CCGC 6 cut(s) 80, 328, 502, 743, 830, 906
SspI AATATT 3 cut(s) 626, 772, 923
SspMI CTAG 2 cut(s) 138, 335
SstI GAGCTC 2 cut(s) 57, 237
StyD4I CCNGG 3 cut(s) 223, 472, 645
StyI CCWWGG 2 cut(s) 181, 685
TaaI ACNGT 6 cut(s) 38, 304, 418, 528, 712, 792
TaiI ACGT 4 cut(s) 11, 213, 719, 758
TaqI TCGA 3 cut(s) 102, 701, 897
TasI AATT 5 cut(s) 25, 204, 219, 406, 697
TatI WGTACW 1 cut(s) 822
TauI GCSGC 4 cut(s) 82, 331, 504, 908
TscAI CASTG 5 cut(s) 421, 539, 557, 738, 888
TseFI GTSAC 2 cut(s) 712, 729
TseI GCWGC 1 cut(s) 390
Tsp45I GTSAC 2 cut(s) 712, 729
TspDTI ATGAA 2 cut(s) 115, 282
TspGWI ACGGA 3 cut(s) 568, 774, 791
TspRI CASTG 5 cut(s) 421, 539, 557, 738, 888
VpaK11BI GGWCC 2 cut(s) 143, 740
XapI RAATTY 2 cut(s) 25, 697
XceI RCATGY 2 cut(s) 642, 682
XhoI CTCGAG 1 cut(s) 896
XmiI GTMKAC 2 cut(s) 420, 723
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 138, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.