FvH4_5g27930

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012465
Physical Location & Seq
Reverse (-)
19150132 .. 19152907
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27930.t1

Sequence Viewer

Length: 1224 bp
ATGGCAAGAAGCAGAGGGGAGAAAGAACAGAGCTTCCACGATAAGAACACGGGTTTTCTCAAACTCTTGCAAGTTGTCTCCTTTTTGGTCGTTTTTGTTGTGGGTGTGGTCATAGGATTGGTAACAAGCGCCCACATCAATCGTCATTTGGGTGCACAAACCGAGACTTATTCCTTTATCAACAACTTCTCCTCCTCCTCCAAGGAGACTACACCACCACCACCACCACCACTGGAGCCTAATTATTGTCCAGCAGTTGTGCAAAATTGCGAGAAGGTTGATTGTTTGAGCATGGCCACATTTCTGGAGCCTAATAAGAACTTGACACATGGTATGTCGGATAATGAGCTGTTTTGGAGAGCTTCATTGGTTCCGGGTACGAAAGAGTATCCGTATGAGAGAGTGCCCAAAGTGGCTTTCTTGTTTATGACTAGAGGGCCGTTGCCTTTGATGCCCTTGTGGGAGAGGTTCTTTCAGGGACATGACAATAAGCTTTTCTCAGTTTATGTGCATGTTGTTCCGGGGTATAGGGTCAATGCATCAACCACTTCTCCTTTTTATCGACGAGAAATCCCTAGTCAGCCTGTTTCTTGGGGAAGCGTTACACTGGTTGATGCAGAGAGGCGCCTTCTAGCTAATGCCTTGCTTGATTTCTCAAATGAGCGCTTTATCCTCCTCTCTGAGAGTTGCATCCCAGTCTATAACTTTCCTACAGTGTACAAGTATCTCACTGGTTCCAAGTATAGCTTTGTTGAATCATACGACGATGCTGGACGTTTTGGCCGTGGGCGTTACAGCCGTAGAATGCTTCCTGATATTCAGCTGTATCAGTGGAGGAAAGGGTCTCAGTGGTTTGAACTTAGCCGCACTTTAGCTGCTATTATAGTCGCAGACGTACGGTACTACACCGTCTTCAGCAAATATTGTTTGCCTGCTTGCTACCCAGATGAGCATTACATGGCAACTTACTTCAATATGTTCCATGGTTCACTGAACTCTAATCGAACCGTGACTTGGGTAGACTGGTCTGTGGGAGGACCACACCCTGCAATGTATGGAGGAGAAAATGTTACAGAAGATTTTGTACGGTCTATTAGGAATAATGGAGCACTTTGTCAGTACAATTCAGAGATGACATCCATTTGTAACCTATTTGCTCGGAAGTTCGCTCCAACAGCATTGGAGCCTTTGCTCGAGCTTGCCTCATCCGTGATGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

408

Amino Acids

46.44

Weight (kDa)

8.03

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 138 - 366 2.3e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 624
AccI GTMKAC 1 cut(s) 1020
AciI CCGC 1 cut(s) 865
AcoI YGGCCR 2 cut(s) 294, 781
AcsI RAATTY 1 cut(s) 1217
AcuI CTGAAG 1 cut(s) 898
AcyI GRCGYC 1 cut(s) 625
AfaI GTAC 6 cut(s) 379, 719, 897, 902, 1086, 1121
AfeI AGCGCT 1 cut(s) 665
AfiI CCNNNNNNNGG 1 cut(s) 1014
AgsI TTSAA 3 cut(s) 755, 857, 973
AjuI GAANNNNNNNTTGG 4 cut(s) 401, 433, 997, 1029
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 9 cut(s) 33, 349, 362, 493, 635, 747, 823, 875, 1198
AluI AGCT 9 cut(s) 33, 349, 362, 493, 635, 747, 823, 875, 1198
Alw21I GWGCWC 2 cut(s) 157, 1111
Alw26I GTCTC 4 cut(s) 82, 158, 200, 849
Alw44I GTGCAC 1 cut(s) 153
Ama87I CYCGRG 1 cut(s) 1193
Aor51HI AGCGCT 1 cut(s) 665
AoxI GGCC 3 cut(s) 294, 437, 781
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 875
ApoI RAATTY 1 cut(s) 1217
AspLEI GCGC 3 cut(s) 131, 627, 666
AspS9I GGNCC 2 cut(s) 437, 1037
AsuC2I CCSGG 2 cut(s) 375, 522
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 1 cut(s) 1037
BaeGI GKGCMC 2 cut(s) 157, 408
BalI TGGCCA 1 cut(s) 296
BanI GGYRCC 1 cut(s) 624
BarI GAAGNNNNNNTAC 2 cut(s) 1068, 1100
BbsI GAAGAC 1 cut(s) 904
Bbv12I GWGCWC 2 cut(s) 157, 1111
BbvI GCAGC 1 cut(s) 862
BccI CCATC 1 cut(s) 1207
BceAI ACGGC 3 cut(s) 424, 768, 783
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 2 cut(s) 375, 522
BcoDI GTCTC 4 cut(s) 82, 158, 200, 849
BfaI CTAG 3 cut(s) 432, 576, 632
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 3 cut(s) 132, 628, 667
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 2 cut(s) 865, 876
BlsI GCNGC 2 cut(s) 866, 877
Bme1390I CCNGG 2 cut(s) 375, 522
Bme18I GGWCC 1 cut(s) 1037
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 2 cut(s) 437, 1037
BmiI GGNNCC 6 cut(s) 237, 309, 372, 626, 736, 1185
BmrFI CCNGG 2 cut(s) 375, 522
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 5 cut(s) 441, 548, 604, 699, 757
BmuI ACTGGG 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 904
BplI GAGNNNNNCTC 2 cut(s) 1187, 1219
BpmI CTGGAG 2 cut(s) 254, 326
BpuMI CCSGG 2 cut(s) 375, 522
BsaHI GRCGYC 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 4 cut(s) 201, 521, 784, 982
BsaXI ACNNNNNCTCC 8 cut(s) 173, 176, 203, 206, 535, 565, 1053, 1083
Bsc4I CCNNNNNNNGG 1 cut(s) 1014
Bse1I ACTGG 5 cut(s) 237, 612, 695, 736, 1028
Bse3DI GCAATG 1 cut(s) 1056
BseDI CCNNGG 4 cut(s) 201, 521, 784, 982
BseGI GGATG 3 cut(s) 690, 1136, 1205
BseLI CCNNNNNNNGG 1 cut(s) 1014
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 3 cut(s) 513, 672, 860
BseNI ACTGG 5 cut(s) 237, 612, 695, 736, 1028
BseRI GAGGAG 5 cut(s) 181, 184, 187, 665, 1074
BseSI GKGCMC 2 cut(s) 157, 408
BseXI GCAGC 1 cut(s) 862
BshFI GGCC 3 cut(s) 296, 439, 783
BshNI GGYRCC 1 cut(s) 624
BsiHKAI GWGCWC 2 cut(s) 157, 1111
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 2 cut(s) 374, 521
BsiWI CGTACG 1 cut(s) 895
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 1014
BsmAI GTCTC 4 cut(s) 82, 158, 200, 849
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 810
BsnI GGCC 3 cut(s) 296, 439, 783
Bso31I GGTCTC 1 cut(s) 849
BsoBI CYCGRG 1 cut(s) 1193
Bsp1286I GDGCHC 3 cut(s) 157, 408, 1111
Bsp1407I TGTACA 1 cut(s) 717
Bsp19I CCATGG 1 cut(s) 982
BspACI CCGC 1 cut(s) 865
BspANI GGCC 3 cut(s) 296, 439, 783
BspCNI CTCAG 3 cut(s) 512, 673, 859
BspLI GGNNCC 6 cut(s) 237, 309, 372, 626, 736, 1185
BspT107I GGYRCC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 849
BsrDI GCAATG 1 cut(s) 1056
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 5 cut(s) 237, 612, 695, 736, 1028
BssECI CCNNGG 4 cut(s) 201, 521, 784, 982
BssNI GRCGYC 1 cut(s) 625
BssT1I CCWWGG 2 cut(s) 201, 982
Bst4CI ACNGT 5 cut(s) 715, 900, 910, 1009, 1089
BstACI GRCGYC 1 cut(s) 625
BstAUI TGTACA 1 cut(s) 717
BstC8I GCNNGC 3 cut(s) 933, 937, 1200
BstDEI CTNAG 4 cut(s) 499, 681, 846, 860
BstDSI CCRYGG 2 cut(s) 784, 982
BstF5I GGATG 3 cut(s) 690, 1136, 1205
BstH2I RGCGCY 3 cut(s) 132, 628, 667
BstHHI GCGC 3 cut(s) 131, 627, 666
BstMAI GTCTC 4 cut(s) 82, 158, 200, 849
BstMWI GCNNNNNNNGC 2 cut(s) 451, 1175
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 2 cut(s) 373, 520
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 2 cut(s) 157, 408
BstV1I GCAGC 1 cut(s) 862
BstV2I GAAGAC 1 cut(s) 904
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 3 cut(s) 296, 439, 783
BtgI CCRYGG 2 cut(s) 784, 982
BtsCI GGATG 3 cut(s) 690, 1136, 1205
BtsIMutI CAGTG 7 cut(s) 230, 605, 720, 729, 836, 854, 989
Cac8I GCNNGC 3 cut(s) 933, 937, 1200
CfoI GCGC 3 cut(s) 131, 627, 666
Cfr13I GGNCC 2 cut(s) 437, 1037
Csp6I GTAC 6 cut(s) 378, 718, 896, 901, 1085, 1120
CviAII CATG 6 cut(s) 292, 329, 482, 512, 958, 983
CviQI GTAC 6 cut(s) 378, 718, 896, 901, 1085, 1120
DdeI CTNAG 4 cut(s) 499, 681, 846, 860
DinI GGCGCC 1 cut(s) 626
EaeI YGGCCR 2 cut(s) 294, 781
Eco130I CCWWGG 2 cut(s) 201, 982
Eco31I GGTCTC 1 cut(s) 849
Eco47I GGWCC 1 cut(s) 1037
Eco47III AGCGCT 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 898
Eco88I CYCGRG 1 cut(s) 1193
EcoT14I CCWWGG 2 cut(s) 201, 982
EcoT22I ATGCAT 1 cut(s) 541
EgeI GGCGCC 1 cut(s) 626
EheI GGCGCC 1 cut(s) 626
ErhI CCWWGG 2 cut(s) 201, 982
FaeI CATG 6 cut(s) 295, 332, 485, 515, 961, 986
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 1 cut(s) 492
FatI CATG 6 cut(s) 291, 328, 481, 511, 957, 982
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 2 cut(s) 865, 876
FokI GGATG 3 cut(s) 677, 1123, 1192
Fsp4HI GCNGC 2 cut(s) 865, 876
FspBI CTAG 3 cut(s) 432, 576, 632
GlaI GCGC 3 cut(s) 130, 626, 665
GluI GCNGC 2 cut(s) 865, 876
GsuI CTGGAG 2 cut(s) 254, 326
HaeII RGCGCY 3 cut(s) 132, 628, 667
HaeIII GGCC 3 cut(s) 296, 439, 783
HapII CCGG 2 cut(s) 374, 521
HhaI GCGC 3 cut(s) 131, 627, 666
Hin1I GRCGYC 1 cut(s) 625
Hin1II CATG 6 cut(s) 295, 332, 485, 515, 961, 986
Hin6I GCGC 3 cut(s) 129, 625, 664
HinP1I GCGC 3 cut(s) 129, 625, 664
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 1 cut(s) 755
HpaII CCGG 2 cut(s) 374, 521
Hpy166II GTNNAC 4 cut(s) 155, 718, 989, 1021
Hpy188I TCNGA 4 cut(s) 340, 682, 1129, 1161
Hpy188III TCNNGA 2 cut(s) 305, 812
Hpy8I GTNNAC 4 cut(s) 155, 718, 989, 1021
Hpy99I CGWCG 2 cut(s) 567, 767
HpyAV CCTTC 2 cut(s) 268, 638
HpyCH4III ACNGT 5 cut(s) 715, 900, 910, 1009, 1089
HpyCH4IV ACGT 2 cut(s) 775, 894
HpyCH4V TGCA 8 cut(s) 70, 155, 262, 511, 539, 617, 690, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 1175
HpyF3I CTNAG 4 cut(s) 499, 681, 846, 860
HpySE526I ACGT 2 cut(s) 775, 894
Hsp92I GRCGYC 1 cut(s) 625
Hsp92II CATG 6 cut(s) 295, 332, 485, 515, 961, 986
HspAI GCGC 3 cut(s) 129, 625, 664
KasI GGCGCC 1 cut(s) 624
LmnI GCTCC 5 cut(s) 235, 307, 1106, 1174, 1183
Lsp1109I GCAGC 1 cut(s) 862
LweI GCATC 5 cut(s) 441, 548, 604, 699, 757
MaeI CTAG 3 cut(s) 432, 576, 632
MaeII ACGT 2 cut(s) 775, 894
MaeIII GTNAC 6 cut(s) 121, 601, 791, 1009, 1069, 1145
MboII GAAGA 2 cut(s) 904, 1088
MhlI GDGCHC 3 cut(s) 157, 408, 1111
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 4 cut(s) 241, 265, 1123, 1217
MluNI TGGCCA 1 cut(s) 296
Mly113I GGCGCC 1 cut(s) 625
MmeI TCCRAC 2 cut(s) 318, 1196
Mox20I TGGCCA 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 541
MscI TGGCCA 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 2 cut(s) 374, 521
MspR9I CCNGG 2 cut(s) 375, 522
Mva1269I GAATGC 1 cut(s) 810
MwoI GCNNNNNNNGC 2 cut(s) 451, 1175
NarI GGCGCC 1 cut(s) 625
NciI CCSGG 2 cut(s) 375, 522
NcoI CCATGG 1 cut(s) 982
NlaIII CATG 6 cut(s) 295, 332, 485, 515, 961, 986
NlaIV GGNNCC 6 cut(s) 237, 309, 372, 626, 736, 1185
NmuCI GTSAC 1 cut(s) 1009
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 1193
PcsI WCGNNNNNNNCGW 1 cut(s) 781
PctI GAATGC 1 cut(s) 810
PfeI GAWTC 1 cut(s) 755
Pfl23II CGTACG 1 cut(s) 895
PkrI GCNGC 2 cut(s) 866, 877
PluTI GGCGCC 1 cut(s) 628
PspLI CGTACG 1 cut(s) 895
PspN4I GGNNCC 6 cut(s) 237, 309, 372, 626, 736, 1185
PspPI GGNCC 2 cut(s) 437, 1037
PspXI VCTCGAGB 1 cut(s) 1193
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 6 cut(s) 379, 719, 897, 902, 1086, 1121
RsaNI GTAC 6 cut(s) 378, 718, 896, 901, 1085, 1120
RseI CAYNNNNRTG 1 cut(s) 150
SatI GCNGC 2 cut(s) 865, 876
Sau96I GGNCC 2 cut(s) 437, 1037
ScrFI CCNGG 2 cut(s) 375, 522
SduI GDGCHC 3 cut(s) 157, 408, 1111
SfaNI GCATC 5 cut(s) 441, 548, 604, 699, 757
SfcI CTRYAG 1 cut(s) 711
SfoI GGCGCC 1 cut(s) 626
Sfr274I CTCGAG 1 cut(s) 1193
SinI GGWCC 1 cut(s) 1037
SlaI CTCGAG 1 cut(s) 1193
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
Sse9I AATT 4 cut(s) 241, 265, 1123, 1217
SsiI CCGC 1 cut(s) 865
SspDI GGCGCC 1 cut(s) 624
SspI AATATT 1 cut(s) 923
SspMI CTAG 3 cut(s) 432, 576, 632
StyD4I CCNGG 2 cut(s) 373, 520
StyI CCWWGG 2 cut(s) 201, 982
TaaI ACNGT 5 cut(s) 715, 900, 910, 1009, 1089
TaiI ACGT 2 cut(s) 778, 897
TaqI TCGA 3 cut(s) 562, 1003, 1194
TasI AATT 4 cut(s) 241, 265, 1123, 1217
TatI WGTACW 2 cut(s) 717, 1119
TauI GCSGC 1 cut(s) 867
TfiI GAWTC 1 cut(s) 755
TscAI CASTG 7 cut(s) 237, 612, 720, 736, 836, 854, 996
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 1 cut(s) 875
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 2 cut(s) 381, 1198
TspRI CASTG 7 cut(s) 237, 612, 720, 736, 836, 854, 996
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 1037
XapI RAATTY 1 cut(s) 1217
XceI RCATGY 1 cut(s) 515
XhoI CTCGAG 1 cut(s) 1193
XmiI GTMKAC 1 cut(s) 1020
XspI CTAG 3 cut(s) 432, 576, 632
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.