Rh7DG328700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012465
Physical Location & Seq
Reverse (-)
39876310 .. 39878707
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG328700.1

Sequence Viewer

Length: 927 bp
ATGGAGACATTTCTGGAGCCTAAGAACTTGACACATGGTATGACGGATAACGAGCTGTTTTGGAGAGCTTCAATGGTTCCGGCAAAGAAAGAATATCCATATCAGAGAGTGCCTAAAGTGGCTTTCATGTTTATGACTAGAGGACCGTTGCCATTGATGCCATTGTGGGAGAGGTTCTTTCAGGGACATGACAAGCTTTTCTCAGTTTATGTGCATGTAGTTCCAGGGTATAAGCTCAATGCATCAACCACCTCTCCTTTTTATCGACGCCAAATCCCTAGTCAGCCTGTTTCTTGGGGAACCATTACACTGATTGATGCAGAGAGGCGCCTTCTAGCTAATGCCTTGCTTGATTTCTCAAATGAGTGCTTTGTCCTCCTATCTGAGAGTTGCATCCCAGTCTATAACTTTCCTACAGTGTACAAGTATCTCATTGGTGCTAATTATAGCTTTGTTGAATCATACGACGATCCTGGACGTTATGGTCGTGGGCGTTACAGCCGTAAAATGCTTCCCGATATTCAGCTGTATCAGTGGAGGAAGGGATCTCAGTGGTTTGAACTTAGCCGCACTTTAGCTACTTTAATAGTCTCAGAAGTACAGTACTACACCGTCTTCAGCAAATATTGTTTGCCTGCTTGCTACCCAGATGAGCATTACATGCCAACTTACTTCAATATGTTCCATGGTTCACTGAACTCTAATCGGACTGTGACTTGGGTAGACTGGTCTTTGGGAGGACCACACCCTGCAACATATGGTGGAGATAATATTACAGAAGATTTTATACGGTCTATAAGGAATAATGGAGCACTTTGTCAGTACAATTCAGAGATGACATCCATTTGTTACCTATTTGCTAGGAAGTTTGCTCCAACAGCATTGGAGCCTCTGCTCGAGCTTGCCTCATCCGTGATGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

308

Amino Acids

35.79

Weight (kDa)

7.6

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 40 - 267 1e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 483
AccB1I GGYRCC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 723
AciI CCGC 1 cut(s) 568
AclWI GGATC 2 cut(s) 464, 553
AcsI RAATTY 1 cut(s) 920
AcuI CTGAAG 1 cut(s) 601
AcyI GRCGYC 2 cut(s) 268, 328
AfaI GTAC 4 cut(s) 422, 600, 605, 824
AgsI TTSAA 4 cut(s) 72, 458, 560, 676
AjnI CCWGG 2 cut(s) 223, 472
AluBI AGCT 9 cut(s) 55, 68, 196, 235, 338, 450, 526, 578, 901
AluI AGCT 9 cut(s) 55, 68, 196, 235, 338, 450, 526, 578, 901
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 2 cut(s) 464, 553
Ama87I CYCGRG 1 cut(s) 896
ApoI RAATTY 1 cut(s) 920
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 2 cut(s) 143, 740
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 2 cut(s) 143, 740
BanI GGYRCC 1 cut(s) 327
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
BbsI GAAGAC 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 814
BccI CCATC 1 cut(s) 910
BceAI ACGGC 1 cut(s) 486
BciT130I CCWGG 2 cut(s) 225, 474
BcoDI GTCTC 1 cut(s) 595
BfaI CTAG 4 cut(s) 138, 279, 335, 861
BfmI CTRYAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 331
BisI GCNGC 1 cut(s) 568
BlsI GCNGC 1 cut(s) 569
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 2 cut(s) 225, 474
Bme18I GGWCC 2 cut(s) 143, 740
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 2 cut(s) 143, 740
BmiI GGNNCC 5 cut(s) 18, 78, 301, 329, 888
BmrFI CCNGG 2 cut(s) 225, 474
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 4 cut(s) 147, 251, 307, 402
BmuI ACTGGG 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 607
BplI GAGNNNNNCTC 2 cut(s) 890, 922
BpmI CTGGAG 1 cut(s) 35
BsaHI GRCGYC 2 cut(s) 268, 328
BsaJI CCNNGG 2 cut(s) 224, 685
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bse1I ACTGG 2 cut(s) 398, 731
BseBI CCWGG 2 cut(s) 225, 474
BseDI CCNNGG 2 cut(s) 224, 685
BseGI GGATG 3 cut(s) 393, 839, 908
BseMII CTCAG 4 cut(s) 216, 375, 563, 606
BseNI ACTGG 2 cut(s) 398, 731
BshNI GGYRCC 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 814
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 198
BsmAI GTCTC 1 cut(s) 595
BsmFI GGGAC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 1 cut(s) 814
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 2 cut(s) 469, 545
Bsp19I CCATGG 1 cut(s) 685
BspACI CCGC 1 cut(s) 568
BspCNI CTCAG 4 cut(s) 215, 376, 562, 605
BspLI GGNNCC 5 cut(s) 18, 78, 301, 329, 888
BspPI GGATC 2 cut(s) 464, 553
BspT107I GGYRCC 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 2 cut(s) 398, 731
BssECI CCNNGG 2 cut(s) 224, 685
BssMI GATC 2 cut(s) 469, 545
BssNI GRCGYC 2 cut(s) 268, 328
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 2 cut(s) 225, 474
Bst4CI ACNGT 6 cut(s) 147, 418, 603, 613, 712, 792
BstACI GRCGYC 2 cut(s) 268, 328
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 3 cut(s) 636, 640, 903
BstDEI CTNAG 6 cut(s) 21, 202, 384, 549, 563, 592
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 3 cut(s) 393, 839, 908
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 472, 548
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 2 cut(s) 469, 545
BstMWI GCNNNNNNNGC 3 cut(s) 157, 661, 878
BstNI CCWGG 2 cut(s) 225, 474
BstNSI RCATGY 2 cut(s) 218, 664
BstSCI CCNGG 2 cut(s) 223, 472
BstSFI CTRYAG 1 cut(s) 414
BstV2I GAAGAC 1 cut(s) 607
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 685
BtsCI GGATG 3 cut(s) 393, 839, 908
BtsIMutI CAGTG 5 cut(s) 308, 423, 539, 557, 692
Cac8I GCNNGC 3 cut(s) 636, 640, 903
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 143, 740
CseI GACGC 1 cut(s) 276
Csp6I GTAC 4 cut(s) 421, 599, 604, 823
CviAII CATG 6 cut(s) 35, 127, 188, 215, 661, 686
CviQI GTAC 4 cut(s) 421, 599, 604, 823
DdeI CTNAG 6 cut(s) 21, 202, 384, 549, 563, 592
DinI GGCGCC 1 cut(s) 329
DpnI GATC 2 cut(s) 471, 547
DpnII GATC 2 cut(s) 469, 545
DrdI GACNNNNNNGTC 1 cut(s) 483
DseDI GACNNNNNNGTC 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 685
Eco47I GGWCC 2 cut(s) 143, 740
Eco57I CTGAAG 1 cut(s) 601
Eco88I CYCGRG 1 cut(s) 896
EcoRII CCWGG 2 cut(s) 223, 472
EcoT14I CCWWGG 1 cut(s) 685
EcoT22I ATGCAT 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 329
EheI GGCGCC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 6 cut(s) 38, 130, 191, 218, 664, 689
FaqI GGGAC 1 cut(s) 198
FatI CATG 6 cut(s) 34, 126, 187, 214, 660, 685
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 1 cut(s) 568
FokI GGATG 3 cut(s) 380, 826, 895
Fsp4HI GCNGC 1 cut(s) 568
FspBI CTAG 4 cut(s) 138, 279, 335, 861
GlaI GCGC 1 cut(s) 329
GluI GCNGC 1 cut(s) 568
GsuI CTGGAG 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 331
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 276
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 2 cut(s) 268, 328
Hin1II CATG 6 cut(s) 38, 130, 191, 218, 664, 689
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 1 cut(s) 458
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 3 cut(s) 421, 692, 724
Hpy188I TCNGA 5 cut(s) 105, 385, 595, 708, 832
Hpy188III TCNNGA 2 cut(s) 14, 515
Hpy8I GTNNAC 3 cut(s) 421, 692, 724
Hpy99I CGWCG 2 cut(s) 270, 470
HpyAV CCTTC 2 cut(s) 341, 535
HpyCH4III ACNGT 6 cut(s) 147, 418, 603, 613, 712, 792
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 5 cut(s) 214, 242, 320, 393, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 661, 878
HpyF3I CTNAG 6 cut(s) 21, 202, 384, 549, 563, 592
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 2 cut(s) 268, 328
Hsp92II CATG 6 cut(s) 38, 130, 191, 218, 664, 689
HspAI GCGC 1 cut(s) 328
KasI GGCGCC 1 cut(s) 327
Kzo9I GATC 2 cut(s) 469, 545
LmnI GCTCC 4 cut(s) 16, 809, 877, 886
LweI GCATC 4 cut(s) 147, 251, 307, 402
MaeI CTAG 4 cut(s) 138, 279, 335, 861
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 3 cut(s) 494, 712, 848
MalI GATC 2 cut(s) 471, 547
MboI GATC 2 cut(s) 469, 545
MboII GAAGA 2 cut(s) 607, 791
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 3 cut(s) 442, 826, 920
Mly113I GGCGCC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 899
MnlI CCTC 9 cut(s) 134, 165, 262, 318, 386, 531, 731, 900, 916
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 1 cut(s) 584
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 225, 474
MvaI CCWGG 2 cut(s) 225, 474
MwoI GCNNNNNNNGC 3 cut(s) 157, 661, 878
NarI GGCGCC 1 cut(s) 328
NcoI CCATGG 1 cut(s) 685
NdeI CATATG 1 cut(s) 757
NdeII GATC 2 cut(s) 469, 545
NlaIII CATG 6 cut(s) 38, 130, 191, 218, 664, 689
NlaIV GGNNCC 5 cut(s) 18, 78, 301, 329, 888
NmuCI GTSAC 1 cut(s) 712
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 2 cut(s) 218, 664
PaeR7I CTCGAG 1 cut(s) 896
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PfeI GAWTC 1 cut(s) 458
PfoI TCCNGGA 1 cut(s) 472
PkrI GCNGC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 223, 472
PspGI CCWGG 2 cut(s) 223, 472
PspN4I GGNNCC 5 cut(s) 18, 78, 301, 329, 888
PspPI GGNCC 2 cut(s) 143, 740
PspXI VCTCGAGB 1 cut(s) 896
PsuI RGATCY 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 4 cut(s) 422, 600, 605, 824
RsaNI GTAC 4 cut(s) 421, 599, 604, 823
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 1 cut(s) 568
Sau3AI GATC 2 cut(s) 469, 545
Sau96I GGNCC 2 cut(s) 143, 740
ScaI AGTACT 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 225, 474
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 4 cut(s) 147, 251, 307, 402
SfcI CTRYAG 1 cut(s) 414
SfoI GGCGCC 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 2 cut(s) 143, 740
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
Sse9I AATT 3 cut(s) 442, 826, 920
SsiI CCGC 1 cut(s) 568
SspDI GGCGCC 1 cut(s) 327
SspI AATATT 2 cut(s) 626, 772
SspMI CTAG 4 cut(s) 138, 279, 335, 861
StyD4I CCNGG 2 cut(s) 223, 472
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 6 cut(s) 147, 418, 603, 613, 712, 792
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 265, 897
TasI AATT 3 cut(s) 442, 826, 920
TatI WGTACW 4 cut(s) 420, 598, 603, 822
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 5 cut(s) 315, 423, 539, 557, 699
TseFI GTSAC 1 cut(s) 712
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 1 cut(s) 115
TspGWI ACGGA 2 cut(s) 59, 901
TspRI CASTG 5 cut(s) 315, 423, 539, 557, 699
VpaK11BI GGWCC 2 cut(s) 143, 740
XapI RAATTY 1 cut(s) 920
XceI RCATGY 2 cut(s) 218, 664
XhoI CTCGAG 1 cut(s) 896
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 4 cut(s) 138, 279, 335, 861
ZrmI AGTACT 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.