Rh7BG322800

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012465
Physical Location & Seq
Reverse (-)
31692331 .. 31696821
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG322800.1

Sequence Viewer

Length: 1221 bp
ATGTATTTCAAAGCCATGGCAAGAAGCAGAGGGGAGAAAGAAGACTGCTACGATAGGAGCACGGGTTTGATCAAACTCTTGCAAGTTCTCTCCTTTCTGGTTGTTTTTGTTGTTGGTGTGGTTATTGGATTAGCAACAAGTGCCCACATTAATCGTCATTTCGGTGCACAAACTGAGATCTATTCGTTTATCAATCACTTCTCCTCCTCCTCCTCCTCCAAGGAGACACCACGCCAAAAAGGGGAGCCCAATTGTACCGTTGTGCAGAGTTGCGAGAGGGTTGATTGTTTGAGCATGGAGGCATTTCTGGAGCCTAAGAACTTGACACATGGTATGCCGGATAATGAGCTGTTTTGGAGAGCTTCAATGGTTCCGGCCAAGAAAGAATATCCATATCAGAGAGTGCCTAAAGTGGCTTTCATGTTTATGGCTAGAGGACCGTTGCCATTGATGCCATTGTGGGAGAGGTTCTTCCAGGGACACGACAAGCTTTTCTCAGTTTATGTGCATGTAGTTCCAGGGTATAAGCTCAATGCATCAACCACCTCTCCTTTTTATCGACGCCAAATCCCTAGTCAGCCTGTTTCTTGGGGAACCATTACACTGATTGATGCAGAGAGGCGCCTTCTAGCTAATGCCTTGCTTGATTTCTCAAATGAGTGCTTTGTCCTCCTATCTGAGAGTTGCATCCCAGTCTATAACTTTCCTACAGTGTACAAGTATCTCATTGGTGCTAATTATAGCTTTGTTGAATCATACGACGATCCTGGACGTTATGGCCGTGGGCGTTACAGCCGTAAAATGCTTCCCGATATTCAGCTGTATCAGTGGAGGAAGGGGTCTCAGTGGTTTGAACTTCGCCGCACTTTAGCTACTTTAATAGTCTCAGAAGTACAGTACTACACCGTCTTCAGCAAATATTGTTTGCCTGCTTGCTACCCAGATGAGCATTACATGCCAACTTACTTCAATATGTTCCATGGTTCACTGAACTCTAATCGGACTGTGACTTGGGTAGACTGGTCTTTGGGAGGACCACACCCTGCAACATATGGTGGAGATAATATTACAGAAGATTTTATACGGTCTATAAGGAATAATGGAGCACTTTGTCAGTACAATTCAGAGATGACATCCATTTGTTACCTATTTGCTAGGAAGTTTGCTCCAACAGCATTGGAGCCTCTGCTCGAGCTTGCCTCATCCGTGATGGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

406

Amino Acids

46.69

Weight (kDa)

8.41

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 138 - 365 2e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 621
AccI GTMKAC 1 cut(s) 1017
AciI CCGC 1 cut(s) 862
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 2 cut(s) 375, 778
AcsI RAATTY 1 cut(s) 1214
AcuI CTGAAG 1 cut(s) 895
AcyI GRCGYC 2 cut(s) 562, 622
AfaI GTAC 5 cut(s) 256, 716, 894, 899, 1118
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 5 cut(s) 10, 366, 752, 854, 970
AjnI CCWGG 3 cut(s) 474, 517, 766
AluBI AGCT 9 cut(s) 349, 362, 490, 529, 632, 744, 820, 872, 1195
AluI AGCT 9 cut(s) 349, 362, 490, 529, 632, 744, 820, 872, 1195
Alw21I GWGCWC 3 cut(s) 62, 169, 1108
Alw26I GTCTC 3 cut(s) 218, 846, 889
Alw44I GTGCAC 1 cut(s) 165
AlwI GGATC 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 1190
AoxI GGCC 2 cut(s) 375, 778
ApaLI GTGCAC 1 cut(s) 165
ApoI RAATTY 1 cut(s) 1214
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
AseI ATTAAT 1 cut(s) 150
AspLEI GCGC 1 cut(s) 624
AspS9I GGNCC 2 cut(s) 437, 1034
AvaI CYCGRG 1 cut(s) 1190
AvaII GGWCC 2 cut(s) 437, 1034
BaeGI GKGCMC 2 cut(s) 145, 169
BanI GGYRCC 1 cut(s) 621
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 1065, 1097
BbsI GAAGAC 2 cut(s) 48, 901
Bbv12I GWGCWC 3 cut(s) 62, 169, 1108
BccI CCATC 1 cut(s) 1204
BceAI ACGGC 2 cut(s) 765, 780
BciT130I CCWGG 3 cut(s) 476, 519, 768
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 3 cut(s) 218, 846, 889
BfaI CTAG 4 cut(s) 432, 573, 629, 1155
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 625
BglII AGATCT 1 cut(s) 177
BisI GCNGC 1 cut(s) 862
BlsI GCNGC 1 cut(s) 863
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 3 cut(s) 476, 519, 768
Bme18I GGWCC 2 cut(s) 437, 1034
BmeT110I CYCGRG 1 cut(s) 1190
BmgT120I GGNCC 2 cut(s) 437, 1034
BmiI GGNNCC 6 cut(s) 246, 312, 372, 595, 623, 1182
BmrFI CCNGG 3 cut(s) 476, 519, 768
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 4 cut(s) 441, 545, 601, 696
BmuI ACTGGG 1 cut(s) 686
BpiI GAAGAC 2 cut(s) 48, 901
BplI GAGNNNNNCTC 2 cut(s) 1184, 1216
BpmI CTGGAG 1 cut(s) 329
BsaHI GRCGYC 2 cut(s) 562, 622
BsaI GGTCTC 1 cut(s) 846
BsaJI CCNNGG 6 cut(s) 15, 219, 475, 518, 781, 979
BsaXI ACNNNNNCTCC 6 cut(s) 49, 79, 188, 218, 532, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 692, 1025
BseBI CCWGG 3 cut(s) 476, 519, 768
BseDI CCNNGG 6 cut(s) 15, 219, 475, 518, 781, 979
BseGI GGATG 3 cut(s) 687, 1133, 1202
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 5 cut(s) 165, 510, 669, 857, 900
BseNI ACTGG 2 cut(s) 692, 1025
BseRI GAGGAG 5 cut(s) 193, 196, 199, 202, 205
BseSI GKGCMC 2 cut(s) 145, 169
BsgI GTGCAG 1 cut(s) 284
BshFI GGCC 2 cut(s) 377, 780
BshNI GGYRCC 1 cut(s) 621
BsiHKAI GWGCWC 3 cut(s) 62, 169, 1108
BsiHKCI CYCGRG 1 cut(s) 1190
BsiSI CCGG 2 cut(s) 338, 374
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 3 cut(s) 218, 846, 889
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 2 cut(s) 377, 780
Bso31I GGTCTC 1 cut(s) 846
BsoBI CYCGRG 1 cut(s) 1190
Bsp1286I GDGCHC 5 cut(s) 62, 145, 169, 249, 1108
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 3 cut(s) 69, 177, 763
Bsp19I CCATGG 2 cut(s) 15, 979
BspACI CCGC 1 cut(s) 862
BspANI GGCC 2 cut(s) 377, 780
BspCNI CTCAG 5 cut(s) 166, 509, 670, 856, 899
BspLI GGNNCC 6 cut(s) 246, 312, 372, 595, 623, 1182
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 692, 1025
BssECI CCNNGG 6 cut(s) 15, 219, 475, 518, 781, 979
BssMI GATC 3 cut(s) 69, 177, 763
BssNI GRCGYC 2 cut(s) 562, 622
BssT1I CCWWGG 3 cut(s) 15, 219, 979
Bst2UI CCWGG 3 cut(s) 476, 519, 768
Bst4CI ACNGT 7 cut(s) 259, 441, 712, 897, 907, 1006, 1086
BstACI GRCGYC 2 cut(s) 562, 622
BstAPI GCANNNNNTGC 2 cut(s) 140, 955
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 3 cut(s) 930, 934, 1197
BstDEI CTNAG 6 cut(s) 174, 315, 496, 678, 843, 886
BstDSI CCRYGG 3 cut(s) 15, 781, 979
BstF5I GGATG 3 cut(s) 687, 1133, 1202
BstH2I RGCGCY 1 cut(s) 625
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 3 cut(s) 72, 180, 766
BstMAI GTCTC 3 cut(s) 218, 846, 889
BstMBI GATC 3 cut(s) 69, 177, 763
BstMWI GCNNNNNNNGC 4 cut(s) 140, 451, 955, 1172
BstNI CCWGG 3 cut(s) 476, 519, 768
BstNSI RCATGY 2 cut(s) 512, 958
BstSCI CCNGG 3 cut(s) 474, 517, 766
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 2 cut(s) 145, 169
BstV2I GAAGAC 2 cut(s) 48, 901
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 2 cut(s) 377, 780
BtgI CCRYGG 3 cut(s) 15, 781, 979
BtsCI GGATG 3 cut(s) 687, 1133, 1202
BtsIMutI CAGTG 5 cut(s) 602, 717, 833, 851, 986
Cac8I GCNNGC 3 cut(s) 930, 934, 1197
CfoI GCGC 1 cut(s) 624
Cfr13I GGNCC 2 cut(s) 437, 1034
CseI GACGC 1 cut(s) 570
Csp6I GTAC 5 cut(s) 255, 715, 893, 898, 1117
CviAII CATG 7 cut(s) 16, 295, 329, 421, 509, 955, 980
CviQI GTAC 5 cut(s) 255, 715, 893, 898, 1117
DdeI CTNAG 6 cut(s) 174, 315, 496, 678, 843, 886
DinI GGCGCC 1 cut(s) 623
DpnI GATC 3 cut(s) 71, 179, 765
DpnII GATC 3 cut(s) 69, 177, 763
EaeI YGGCCR 2 cut(s) 375, 778
Eco130I CCWWGG 3 cut(s) 15, 219, 979
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 846
Eco47I GGWCC 2 cut(s) 437, 1034
Eco57I CTGAAG 1 cut(s) 895
Eco88I CYCGRG 1 cut(s) 1190
EcoRII CCWGG 3 cut(s) 474, 517, 766
EcoT14I CCWWGG 3 cut(s) 15, 219, 979
EcoT22I ATGCAT 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 249
EgeI GGCGCC 1 cut(s) 623
EheI GGCGCC 1 cut(s) 623
ErhI CCWWGG 3 cut(s) 15, 219, 979
FaeI CATG 7 cut(s) 19, 298, 332, 424, 512, 958, 983
FaqI GGGAC 1 cut(s) 492
FatI CATG 7 cut(s) 15, 294, 328, 420, 508, 954, 979
FauNDI CATATG 1 cut(s) 1051
FbaI TGATCA 1 cut(s) 69
FblI GTMKAC 1 cut(s) 1017
Fnu4HI GCNGC 1 cut(s) 862
FokI GGATG 3 cut(s) 674, 1120, 1189
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 862
FspBI CTAG 4 cut(s) 432, 573, 629, 1155
GlaI GCGC 1 cut(s) 623
GluI GCNGC 1 cut(s) 862
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 625
HaeIII GGCC 2 cut(s) 377, 780
HapII CCGG 2 cut(s) 338, 374
HgaI GACGC 1 cut(s) 570
HhaI GCGC 1 cut(s) 624
Hin1I GRCGYC 2 cut(s) 562, 622
Hin1II CATG 7 cut(s) 19, 298, 332, 424, 512, 958, 983
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 752
HpaII CCGG 2 cut(s) 338, 374
Hpy166II GTNNAC 4 cut(s) 167, 715, 986, 1018
Hpy188I TCNGA 5 cut(s) 399, 679, 889, 1002, 1126
Hpy188III TCNNGA 2 cut(s) 308, 809
Hpy8I GTNNAC 4 cut(s) 167, 715, 986, 1018
Hpy99I CGWCG 2 cut(s) 564, 764
HpyAV CCTTC 2 cut(s) 635, 829
HpyCH4III ACNGT 7 cut(s) 259, 441, 712, 897, 907, 1006, 1086
HpyCH4IV ACGT 1 cut(s) 772
HpyCH4V TGCA 8 cut(s) 82, 167, 265, 508, 536, 614, 687, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 451, 955, 1172
HpyF3I CTNAG 6 cut(s) 174, 315, 496, 678, 843, 886
HpySE526I ACGT 1 cut(s) 772
Hsp92I GRCGYC 2 cut(s) 562, 622
Hsp92II CATG 7 cut(s) 19, 298, 332, 424, 512, 958, 983
HspAI GCGC 1 cut(s) 622
KasI GGCGCC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 3 cut(s) 69, 177, 763
LmnI GCTCC 6 cut(s) 57, 244, 310, 1103, 1171, 1180
LweI GCATC 4 cut(s) 441, 545, 601, 696
MaeI CTAG 4 cut(s) 432, 573, 629, 1155
MaeII ACGT 1 cut(s) 772
MaeIII GTNAC 3 cut(s) 788, 1006, 1142
MalI GATC 3 cut(s) 71, 179, 765
MboI GATC 3 cut(s) 69, 177, 763
MboII GAAGA 4 cut(s) 53, 463, 901, 1085
MfeI CAATTG 1 cut(s) 250
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 5 cut(s) 62, 145, 169, 249, 1108
MluCI AATT 4 cut(s) 250, 736, 1120, 1214
Mly113I GGCGCC 1 cut(s) 622
MmeI TCCRAC 1 cut(s) 1193
Mph1103I ATGCAT 1 cut(s) 538
MseI TTAA 2 cut(s) 150, 878
MslI CAYNNNNRTG 2 cut(s) 162, 425
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 2 cut(s) 338, 374
MspR9I CCNGG 3 cut(s) 476, 519, 768
MunI CAATTG 1 cut(s) 250
MvaI CCWGG 3 cut(s) 476, 519, 768
MwoI GCNNNNNNNGC 4 cut(s) 140, 451, 955, 1172
NarI GGCGCC 1 cut(s) 622
NcoI CCATGG 2 cut(s) 15, 979
NdeI CATATG 1 cut(s) 1051
NdeII GATC 3 cut(s) 69, 177, 763
NlaIII CATG 7 cut(s) 19, 298, 332, 424, 512, 958, 983
NlaIV GGNNCC 6 cut(s) 246, 312, 372, 595, 623, 1182
NmuCI GTSAC 1 cut(s) 1006
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 2 cut(s) 512, 958
PaeR7I CTCGAG 1 cut(s) 1190
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PfeI GAWTC 1 cut(s) 752
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 1 cut(s) 863
PluTI GGCGCC 1 cut(s) 625
PshBI ATTAAT 1 cut(s) 150
Psp6I CCWGG 3 cut(s) 474, 517, 766
PspGI CCWGG 3 cut(s) 474, 517, 766
PspN4I GGNNCC 6 cut(s) 246, 312, 372, 595, 623, 1182
PspPI GGNCC 2 cut(s) 437, 1034
PspXI VCTCGAGB 1 cut(s) 1190
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 5 cut(s) 256, 716, 894, 899, 1118
RsaNI GTAC 5 cut(s) 255, 715, 893, 898, 1117
RseI CAYNNNNRTG 2 cut(s) 162, 425
SaqAI TTAA 2 cut(s) 150, 878
SatI GCNGC 1 cut(s) 862
Sau3AI GATC 3 cut(s) 69, 177, 763
Sau96I GGNCC 2 cut(s) 437, 1034
ScaI AGTACT 1 cut(s) 899
ScrFI CCNGG 3 cut(s) 476, 519, 768
SduI GDGCHC 5 cut(s) 62, 145, 169, 249, 1108
SfaNI GCATC 4 cut(s) 441, 545, 601, 696
SfcI CTRYAG 1 cut(s) 708
SfoI GGCGCC 1 cut(s) 623
Sfr274I CTCGAG 1 cut(s) 1190
SinI GGWCC 2 cut(s) 437, 1034
SlaI CTCGAG 1 cut(s) 1190
SmiMI CAYNNNNRTG 2 cut(s) 162, 425
SmlI CTYRAG 1 cut(s) 1190
SmoI CTYRAG 1 cut(s) 1190
Sse9I AATT 4 cut(s) 250, 736, 1120, 1214
SsiI CCGC 1 cut(s) 862
SspDI GGCGCC 1 cut(s) 621
SspI AATATT 2 cut(s) 920, 1066
SspMI CTAG 4 cut(s) 432, 573, 629, 1155
StyD4I CCNGG 3 cut(s) 474, 517, 766
StyI CCWWGG 3 cut(s) 15, 219, 979
TaaI ACNGT 7 cut(s) 259, 441, 712, 897, 907, 1006, 1086
TaiI ACGT 1 cut(s) 775
TaqI TCGA 2 cut(s) 559, 1191
TasI AATT 4 cut(s) 250, 736, 1120, 1214
TatI WGTACW 4 cut(s) 714, 892, 897, 1116
TauI GCSGC 1 cut(s) 864
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 2 cut(s) 150, 878
Tru9I TTAA 2 cut(s) 150, 878
TscAI CASTG 5 cut(s) 609, 717, 833, 851, 993
TseFI GTSAC 1 cut(s) 1006
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 1 cut(s) 409
TspGWI ACGGA 1 cut(s) 1195
TspRI CASTG 5 cut(s) 609, 717, 833, 851, 993
VneI GTGCAC 1 cut(s) 165
VpaK11BI GGWCC 2 cut(s) 437, 1034
VspI ATTAAT 1 cut(s) 150
XapI RAATTY 1 cut(s) 1214
XceI RCATGY 2 cut(s) 512, 958
XhoI CTCGAG 1 cut(s) 1190
XmiI GTMKAC 1 cut(s) 1017
XspI CTAG 4 cut(s) 432, 573, 629, 1155
ZrmI AGTACT 1 cut(s) 899
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.