Rorug07G0189700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012465
Physical Location & Seq
Reverse (-)
16019036 .. 16022553
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0189700.1

Sequence Viewer

Length: 1590 bp
ATGTGGTCATCAATGTGGGATACTAGCAGTGAGAAACATGGAGATCTCAAAGCAGCTCTCACCATCTTCGCCATCATCATGACAACTGTTAGTTTCTGGTTCCTCCAGACTTGGAATAAGCACTCGAGGAACTCGAAACCAGCTCCATTGCCGCCTGGCCCTCATGGTGTTCCACTACTCGGTTACCTTCCATTTTTAGGTACCAACCATCACCACCAGTTCACAGATTTGTCAAGGGTTTATGGCCCTATTTACAGGATCCAACTCGGTACCAAACTAGTCATTGTGGTCAGCTCACCATCGCTGGTGAAAGAAGTGGTTCGTGACCAAGACACGATATTTTTCTACCGCAACGTTAAAGTGGCACAACGAATTCTCTCATATGGAGGATCCGATATAGTCTTTGGGACCCACGGTCCAGAGTGGAGGAAGATGCGCAAGATGCTCGCGAGTCAAATGCTAAGCAAAACCAACCTTGACGATTGTTATGCTCTGAGAAAACAAGAGGTTGTGAAGTCAATTAGGCATGTTTATAATGAAAAAATTGGGACCCCAATTGATTTTGGGCAGTTTGTAATGTCCACAACCTCCAACACATTCTCGTGCATGTTATGGGGTGGGACTCTACAAGGAGAGAAGGCATCTGATGTTGGATCCGAGTTTAGAAAGGTAGTGATGGAAATGATCTGGTTACTTCAGAAACCAAACGTCTCGGACTTTTTCCCTGTGCTTGCTAGGTTTGATATACAGGGAATTAGGAGCCGAGCAAAGAAGCTTCAGTCAGTAACTGAAAACATGTTCGATTCGATCATAGAAACTCAGATGAATAAGGATAAAAATGAGGGAGTACCAAAAAAACATGAAGGGAAGGGCTTTCTGCAGTTCCTCTTGGAGAATAATATAAATCAAGATAGTGCAACATCGCTTACAATGAAACAAGTGAAGGCCTTGCTCACGGACATAGTGGTGGCAGGAAACGACACCACAGCAGGAATAATTGAATGGGCAATGTCGGAGCTGATACAACATCCGGATGAATTGAGAAAAGTCCAAGAAGAACTAACAAAAATTGTGGGGATAAACAACTTAGTTGAAGAGTTTCATTTGCCAAAATTACATTACTTAGATGCAGTGATCAAGGAGACATTTCGTTTGCACCCTGTACTGCCGCTTCTAGCGCCCCGATGTTCAAGCCAATCCACCACCATTGGTGGCTATTACATACCTAAAGGTTCCATTGTTTTTCTCAATGCTTGGGCCATACACAGAGATTCAAGTGTCTGGGACGATCCCTTGGAATTTAGACCCTGGAGGTTCCTAAACACTGATACAAGCAATAGCTTTGGGTACCAAGGAAATAACTTTCACTATCTTCCATTTGGCTCAGGGAGAAGAATATGTCCTGGGATTCCCTTAGCAGAGAGGATACTAGTCTATGTGCTAGCTTCATTTTTGCATTCGTTTGAGTGGACATTGCCTAATGACGCAACGGTTGACCTTTCAGACAAATTTGGGATTGTGACAAAGAAAAAGGCTCCACTTATTGTTGTACCAACACCCAGGTTATCCAATTTGGAGCTCTATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

529

Amino Acids

60.01

Weight (kDa)

8.97

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 51 - 512 1.2e-96 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10280
fragaria_vesca FvH4_5g27930
malus_domestica MD08G1246800.v1.1 MD15G1441600.v1.1
prunus_persica Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1 Prupe.1G583700_v2.0.a1
pyrus_communis pycom08g21390 pycom15g38960
rosa_chinensis RchiOBHm_Chr7g0220111
rosa_laevigata RLG00000002256
rosa_multiflora Rmu_sc0016657.1_g000006
rosa_roxburghii Rroxscaffold_3G00239780
rosa_rugosa Rorug07G0189700
rosa_samantha Rh7AG332600 Rh7BG322800 Rh7CG350100 Rh7DG328700
rosa_wichuraiana Rw7G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
Acc16I TGCGCA 1 cut(s) 437
Acc65I GGTACC 3 cut(s) 200, 269, 1347
AccB1I GGYRCC 3 cut(s) 200, 269, 1347
AccII CGCG 1 cut(s) 449
AccIII TCCGGA 1 cut(s) 1030
AciI CCGC 3 cut(s) 152, 349, 1169
AclI AACGTT 1 cut(s) 354
AclWI GGATC 7 cut(s) 253, 266, 384, 397, 648, 661, 1283
AcsI RAATTY 3 cut(s) 372, 1298, 1508
AcuI CTGAAG 2 cut(s) 680, 761
AfaI GTAC 6 cut(s) 202, 271, 849, 1164, 1349, 1551
AfiI CCNNNNNNNGG 2 cut(s) 179, 197
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 4 cut(s) 1001, 1094, 1191, 1275
AhdI GACNNNNNGTC 1 cut(s) 414
AhlI ACTAGT 2 cut(s) 277, 1429
AjnI CCWGG 4 cut(s) 154, 1307, 1402, 1559
AluBI AGCT 8 cut(s) 56, 143, 294, 775, 1018, 1341, 1445, 1579
AluI AGCT 8 cut(s) 56, 143, 294, 775, 1018, 1341, 1445, 1579
Alw21I GWGCWC 1 cut(s) 1581
Alw26I GTCTC 2 cut(s) 715, 1136
AlwI GGATC 7 cut(s) 253, 266, 384, 397, 648, 661, 1283
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 124
Aor13HI TCCGGA 1 cut(s) 1030
AoxI GGCC 4 cut(s) 157, 244, 945, 1257
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 3 cut(s) 372, 1298, 1508
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
Asp700I GAANNNNTTC 2 cut(s) 318, 1098
Asp718I GGTACC 3 cut(s) 200, 269, 1347
AspLEI GCGC 2 cut(s) 438, 1180
AspS9I GGNCC 6 cut(s) 158, 245, 408, 416, 549, 1257
AsuHPI GGTGA 4 cut(s) 52, 203, 288, 319
AsuNHI GCTAGC 1 cut(s) 1441
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 3 cut(s) 408, 416, 549
BamHI GGATCC 3 cut(s) 258, 389, 653
BanI GGYRCC 3 cut(s) 200, 269, 1347
BanII GRGCYC 1 cut(s) 1581
BarI GAAGNNNNNNTAC 2 cut(s) 1155, 1187
BauI CACGAG 1 cut(s) 601
Bbv12I GWGCWC 1 cut(s) 1581
BbvI GCAGC 1 cut(s) 65
BccI CCATC 5 cut(s) 71, 80, 216, 307, 670
BcgI CGANNNNNNTGC 6 cut(s) 427, 439, 461, 470, 473, 504
BciT130I CCWGG 4 cut(s) 156, 1309, 1404, 1561
BciVI GTATCC 2 cut(s) 13, 1419
BclI TGATCA 1 cut(s) 1134
BcoDI GTCTC 2 cut(s) 715, 1136
BcuI ACTAGT 2 cut(s) 277, 1429
BfaI CTAG 6 cut(s) 24, 278, 735, 1175, 1430, 1442
BfmI CTRYAG 1 cut(s) 878
BfoI RGCGCY 1 cut(s) 1181
BfuI GTATCC 2 cut(s) 13, 1419
BglII AGATCT 1 cut(s) 43
BisI GCNGC 3 cut(s) 54, 152, 1169
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 3 cut(s) 55, 153, 1170
Bme1390I CCNGG 4 cut(s) 156, 1309, 1404, 1561
Bme18I GGWCC 3 cut(s) 408, 416, 549
BmeRI GACNNNNNGTC 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 6 cut(s) 158, 245, 408, 416, 549, 1257
BmrFI CCNGG 4 cut(s) 156, 1309, 1404, 1561
BmsI GCATC 4 cut(s) 423, 432, 650, 1117
BmtI GCTAGC 1 cut(s) 1445
BpmI CTGGAG 2 cut(s) 89, 1330
Bpu10I CCTNAGC 2 cut(s) 1384, 1414
Bpu1102I GCTNAGC 1 cut(s) 461
BsaJI CCNNGG 6 cut(s) 412, 1293, 1307, 1351, 1403, 1559
BsaWI WCCGGW 1 cut(s) 1030
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 197
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 3 cut(s) 146, 1014, 1472
BseAI TCCGGA 1 cut(s) 1030
BseBI CCWGG 4 cut(s) 156, 1309, 1404, 1561
BseDI CCNNGG 6 cut(s) 412, 1293, 1307, 1351, 1403, 1559
BseGI GGATG 2 cut(s) 1027, 1039
BseLI CCNNNNNNNGG 2 cut(s) 179, 197
BseMI GCAATG 3 cut(s) 146, 1014, 1472
BseMII CTCAG 3 cut(s) 485, 833, 1398
BseNI ACTGG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 65
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 4 cut(s) 159, 246, 947, 1259
BshNI GGYRCC 3 cut(s) 200, 269, 1347
BsiHKAI GWGCWC 1 cut(s) 1581
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 1 cut(s) 1031
BslFI GGGAC 4 cut(s) 421, 562, 634, 1298
BslI CCNNNNNNNGG 2 cut(s) 179, 197
BsmAI GTCTC 2 cut(s) 715, 1136
BsmBI CGTCTC 1 cut(s) 715
BsmFI GGGAC 4 cut(s) 421, 562, 634, 1298
BsmI GAATGC 1 cut(s) 1456
BsnI GGCC 4 cut(s) 159, 246, 947, 1259
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 1581
Bsp13I TCCGGA 1 cut(s) 1030
Bsp143I GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
Bsp1720I GCTNAGC 1 cut(s) 461
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 3 cut(s) 152, 349, 1169
BspANI GGCC 4 cut(s) 159, 246, 947, 1259
BspCNI CTCAG 3 cut(s) 486, 832, 1397
BspEI TCCGGA 1 cut(s) 1030
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 882
BspOI GCTAGC 1 cut(s) 1445
BspPI GGATC 7 cut(s) 253, 266, 384, 397, 648, 661, 1283
BspT107I GGYRCC 3 cut(s) 200, 269, 1347
BsrDI GCAATG 3 cut(s) 146, 1014, 1472
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 6 cut(s) 412, 1293, 1307, 1351, 1403, 1559
BssMI GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
BssSI CACGAG 1 cut(s) 601
BssT1I CCWWGG 2 cut(s) 1293, 1351
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 4 cut(s) 156, 1309, 1404, 1561
Bst4CI ACNGT 3 cut(s) 88, 416, 1492
Bst6I CTCTTC 1 cut(s) 1089
BstC8I GCNNGC 3 cut(s) 447, 732, 1443
BstDEI CTNAG 7 cut(s) 461, 494, 819, 1087, 1123, 1384, 1414
BstDSI CCRYGG 1 cut(s) 412
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 2 cut(s) 1027, 1039
BstFNI CGCG 1 cut(s) 449
BstH2I RGCGCY 1 cut(s) 1181
BstHHI GCGC 2 cut(s) 438, 1180
BstKTI GATC 8 cut(s) 46, 261, 392, 656, 687, 810, 1137, 1291
BstMAI GTCTC 2 cut(s) 715, 1136
BstMBI GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
BstMWI GCNNNNNNNGC 2 cut(s) 442, 1177
BstNI CCWGG 4 cut(s) 156, 1309, 1404, 1561
BstNSI RCATGY 3 cut(s) 530, 610, 799
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 4 cut(s) 154, 1307, 1402, 1559
BstSFI CTRYAG 1 cut(s) 878
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 4 cut(s) 43, 258, 389, 653
BstYI RGATCY 4 cut(s) 43, 258, 389, 653
BsuI GTATCC 2 cut(s) 13, 1419
BsuRI GGCC 4 cut(s) 159, 246, 947, 1259
BtgI CCRYGG 1 cut(s) 412
BtgZI GCGATG 2 cut(s) 285, 906
BtsCI GGATG 2 cut(s) 1027, 1039
BtsI GCAGTG 2 cut(s) 34, 1137
BtsIMutI CAGTG 3 cut(s) 34, 1137, 1323
BtuMI TCGCGA 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 447, 732, 1443
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 78
CfoI GCGC 2 cut(s) 438, 1180
Cfr13I GGNCC 6 cut(s) 158, 245, 408, 416, 549, 1257
CseI GACGC 1 cut(s) 1493
Csp6I GTAC 6 cut(s) 201, 270, 848, 1163, 1348, 1550
CspCI CAANNNNNGTGG 4 cut(s) 1053, 1088, 1189, 1224
CviAII CATG 7 cut(s) 38, 79, 164, 527, 607, 796, 860
CviQI GTAC 6 cut(s) 201, 270, 848, 1163, 1348, 1550
DdeI CTNAG 7 cut(s) 461, 494, 819, 1087, 1123, 1384, 1414
DpnI GATC 8 cut(s) 45, 260, 391, 655, 686, 809, 1136, 1290
DpnII GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
DriI GACNNNNNGTC 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 1089
Eam1105I GACNNNNNGTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 1089
Ecl136II GAGCTC 1 cut(s) 1579
Eco130I CCWWGG 2 cut(s) 1293, 1351
Eco147I AGGCCT 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 1581
Eco47I GGWCC 3 cut(s) 408, 416, 549
Eco53kI GAGCTC 1 cut(s) 1579
Eco57I CTGAAG 2 cut(s) 680, 761
Eco88I CYCGRG 1 cut(s) 124
Eco91I GGTNACC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 1579
EcoO109I RGGNCCY 2 cut(s) 408, 549
EcoO65I GGTNACC 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 4 cut(s) 154, 1307, 1402, 1559
EcoT14I CCWWGG 2 cut(s) 1293, 1351
EcoT38I GRGCYC 1 cut(s) 1581
ErhI CCWWGG 2 cut(s) 1293, 1351
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 7 cut(s) 41, 82, 167, 530, 610, 799, 863
FaqI GGGAC 4 cut(s) 421, 562, 634, 1298
FatI CATG 7 cut(s) 37, 78, 163, 526, 606, 795, 859
FauNDI CATATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 1134
Fnu4HI GCNGC 3 cut(s) 54, 152, 1169
FokI GGATG 2 cut(s) 1014, 1046
FriOI GRGCYC 1 cut(s) 1581
Fsp4HI GCNGC 3 cut(s) 54, 152, 1169
FspBI CTAG 6 cut(s) 24, 278, 735, 1175, 1430, 1442
FspI TGCGCA 1 cut(s) 437
GlaI GCGC 2 cut(s) 437, 1179
GluI GCNGC 3 cut(s) 54, 152, 1169
GsuI CTGGAG 2 cut(s) 89, 1330
HaeII RGCGCY 1 cut(s) 1181
HaeIII GGCC 4 cut(s) 159, 246, 947, 1259
HapII CCGG 1 cut(s) 1031
HgaI GACGC 1 cut(s) 1493
HhaI GCGC 2 cut(s) 438, 1180
Hin1II CATG 7 cut(s) 41, 82, 167, 530, 610, 799, 863
Hin6I GCGC 2 cut(s) 436, 1178
HinP1I GCGC 2 cut(s) 436, 1178
HincII GTYRAC 1 cut(s) 1495
HindII GTYRAC 1 cut(s) 1495
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 5 cut(s) 451, 622, 803, 1271, 1408
HpaII CCGG 1 cut(s) 1031
HphI GGTGA 4 cut(s) 52, 203, 288, 319
Hpy166II GTNNAC 4 cut(s) 222, 582, 1470, 1495
Hpy188I TCNGA 9 cut(s) 394, 495, 646, 658, 699, 715, 822, 1015, 1504
Hpy188III TCNNGA 7 cut(s) 79, 106, 323, 419, 448, 908, 1031
Hpy8I GTNNAC 4 cut(s) 222, 582, 1470, 1495
HpyAV CCTTC 5 cut(s) 197, 631, 857, 862, 937
HpyCH4III ACNGT 3 cut(s) 88, 416, 1492
HpyCH4IV ACGT 2 cut(s) 354, 708
HpyCH4V TGCA 6 cut(s) 606, 880, 917, 1130, 1156, 1456
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 1177
HpyF3I CTNAG 7 cut(s) 461, 494, 819, 1087, 1123, 1384, 1414
HpySE526I ACGT 2 cut(s) 354, 708
Hsp92II CATG 7 cut(s) 41, 82, 167, 530, 610, 799, 863
HspAI GCGC 2 cut(s) 436, 1178
KflI GGGWCCC 2 cut(s) 408, 549
Kpn2I TCCGGA 1 cut(s) 1030
KpnI GGTACC 3 cut(s) 204, 273, 1351
Ksp22I TGATCA 1 cut(s) 1134
Kzo9I GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
LmnI GCTCC 5 cut(s) 148, 759, 1015, 1540, 1576
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 4 cut(s) 423, 432, 650, 1117
MaeI CTAG 6 cut(s) 24, 278, 735, 1175, 1430, 1442
MaeII ACGT 2 cut(s) 354, 708
MaeIII GTNAC 5 cut(s) 182, 323, 690, 784, 1519
MalI GATC 8 cut(s) 45, 260, 391, 655, 686, 809, 1136, 1290
MboI GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
MboII GAAGA 6 cut(s) 58, 442, 1067, 1106, 1364, 1404
MfeI CAATTG 1 cut(s) 555
MflI RGATCY 4 cut(s) 43, 258, 389, 653
MhlI GDGCHC 1 cut(s) 1581
MlyI GAGTC 2 cut(s) 460, 616
MmeI TCCRAC 4 cut(s) 286, 615, 631, 993
MroI TCCGGA 1 cut(s) 1030
MroXI GAANNNNTTC 2 cut(s) 318, 1098
MseI TTAA 2 cut(s) 357, 1588
MslI CAYNNNNRTG 5 cut(s) 13, 77, 601, 965, 1032
MspI CCGG 1 cut(s) 1031
MspR9I CCNGG 4 cut(s) 156, 1309, 1404, 1561
MunI CAATTG 1 cut(s) 555
Mva1269I GAATGC 1 cut(s) 1456
MvaI CCWGG 4 cut(s) 156, 1309, 1404, 1561
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 442, 1177
NdeI CATATG 1 cut(s) 382
NdeII GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
NheI GCTAGC 1 cut(s) 1441
NlaIII CATG 7 cut(s) 41, 82, 167, 530, 610, 799, 863
NmeAIII GCCGAG 1 cut(s) 788
NmuCI GTSAC 2 cut(s) 323, 1519
NruI TCGCGA 1 cut(s) 449
NsbI TGCGCA 1 cut(s) 437
NspI RCATGY 3 cut(s) 530, 610, 799
PaeR7I CTCGAG 1 cut(s) 124
PagI TCATGA 1 cut(s) 78
PceI AGGCCT 1 cut(s) 947
PciI ACATGT 1 cut(s) 795
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 1456
PdmI GAANNNNTTC 2 cut(s) 318, 1098
PfeI GAWTC 3 cut(s) 803, 1271, 1408
PkrI GCNGC 3 cut(s) 55, 153, 1170
PleI GAGTC 2 cut(s) 459, 616
PpsI GAGTC 2 cut(s) 459, 616
PpuMI RGGWCCY 2 cut(s) 408, 549
PscI ACATGT 1 cut(s) 795
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 1581
Psp1406I AACGTT 1 cut(s) 354
Psp5II RGGWCCY 2 cut(s) 408, 549
Psp6I CCWGG 4 cut(s) 154, 1307, 1402, 1559
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 4 cut(s) 154, 1307, 1402, 1559
PspPI GGNCC 6 cut(s) 158, 245, 408, 416, 549, 1257
PspPPI RGGWCCY 2 cut(s) 408, 549
PspXI VCTCGAGB 1 cut(s) 124
PstI CTGCAG 1 cut(s) 882
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 43, 258, 389, 653
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 6 cut(s) 202, 271, 849, 1164, 1349, 1551
RsaNI GTAC 6 cut(s) 201, 270, 848, 1163, 1348, 1550
RseI CAYNNNNRTG 5 cut(s) 13, 77, 601, 965, 1032
SacI GAGCTC 1 cut(s) 1581
SaqAI TTAA 2 cut(s) 357, 1588
SatI GCNGC 3 cut(s) 54, 152, 1169
Sau3AI GATC 8 cut(s) 43, 258, 389, 653, 684, 807, 1134, 1288
Sau96I GGNCC 6 cut(s) 158, 245, 408, 416, 549, 1257
SchI GAGTC 2 cut(s) 460, 616
ScrFI CCNGG 4 cut(s) 156, 1309, 1404, 1561
SduI GDGCHC 1 cut(s) 1581
SfaNI GCATC 4 cut(s) 423, 432, 650, 1117
SfcI CTRYAG 1 cut(s) 878
Sfr274I CTCGAG 1 cut(s) 124
SinI GGWCC 3 cut(s) 408, 416, 549
SlaI CTCGAG 1 cut(s) 124
SmiMI CAYNNNNRTG 5 cut(s) 13, 77, 601, 965, 1032
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
SpeI ACTAGT 2 cut(s) 277, 1429
SseBI AGGCCT 1 cut(s) 947
SsiI CCGC 3 cut(s) 152, 349, 1169
SspMI CTAG 6 cut(s) 24, 278, 735, 1175, 1430, 1442
SstI GAGCTC 1 cut(s) 1581
StuI AGGCCT 1 cut(s) 947
StyD4I CCNGG 4 cut(s) 154, 1307, 1402, 1559
StyI CCWWGG 2 cut(s) 1293, 1351
TaaI ACNGT 3 cut(s) 88, 416, 1492
TaiI ACGT 2 cut(s) 357, 711
TaqI TCGA 4 cut(s) 125, 134, 801, 806
TatI WGTACW 1 cut(s) 1162
TauI GCSGC 2 cut(s) 154, 1171
TfiI GAWTC 3 cut(s) 803, 1271, 1408
Tru1I TTAA 2 cut(s) 357, 1588
Tru9I TTAA 2 cut(s) 357, 1588
TscAI CASTG 3 cut(s) 34, 1137, 1330
TseFI GTSAC 2 cut(s) 323, 1519
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 2 cut(s) 323, 1519
TspDTI ATGAA 7 cut(s) 552, 839, 876, 947, 1050, 1091, 1437
TspGWI ACGGA 1 cut(s) 971
TspRI CASTG 3 cut(s) 34, 1137, 1330
VpaK11BI GGWCC 3 cut(s) 408, 416, 549
XapI RAATTY 3 cut(s) 372, 1298, 1508
XceI RCATGY 3 cut(s) 530, 610, 799
XhoI CTCGAG 1 cut(s) 124
XmnI GAANNNNTTC 2 cut(s) 318, 1098
XspI CTAG 6 cut(s) 24, 278, 735, 1175, 1430, 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.