AT1G10540

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005215
Physical Location & Seq
Reverse (-)
3474858 .. 3478028
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10540.2

Sequence Viewer

Length: 1620 bp
ATGGCAGGTGATGGCGTAGAAAATGCAAAGCCACCGCAGAAACAGGAAGATCTTCAGCCTCATCCGGTGAAGGATCAACTTTACGGTATCACTTACTGCCTCACAAGTCCTCCTCCTTGGCCGGAGACAATACTTCTGGGGTTTCAACACTACTTAGTGATGCTTGGAACAACTGTTCTCATTCCAACAATGTTAGTTTCAAAGATTGATGCAAGAAACGAAGATAAGGTAAAGTTGATTCAGACGTTGCTCTTTGTGTCTGGAATCAACACGTTATTTCAAAGCTTCTTTGGGACTCGTCTTCCTGCTGTCATTGGAGCTTCTTACTCTTATGTGCCAACTACAATGTCAATCGTCTTAGCTGCTCGTTACAATGACATTATGGATCCTCAAAAGAGATTTGAACAGATCATGCGTGGAATCCAAGGTGCTCTTATTATTGCCTCATTTCTTCATATCCTTGTTGGTTTCAGTGGCCTTTGGCGCAATGTTACTAGGTTTTTGAGTCCCTTATCTGCAGTTCCTCTTGTAGCCTTTTCTGGCTTTGGACTCTATGAACAAGGATTCCCTATGCTAGCAAAATGCATTGAGATTGGACTGCCTGAGATCATACTTCTTGTCATATTCTCACAGTACATTCCTCATCTGATGCAAGGAGAAACATGTTCTAACTTCTTCCACCGATTTGCTGTGATATTCTCAGTGGTGATTGTGTGGCTTTATGCATACATTCTAACCATTGGTGGGGCTTATAGTAACACAGAAATCAATACTCAAATCAGTTGCAGAACCGACCGTGCTGGCATCATTAGCGCTTCTCCATGGATTAGAGTGCCTCACCCTATTCAATGGGGAGGAGCTCCAACTTTCAATGCAGGGGATATTTTTGCTATGATGGCTGCTTCTTTCGTTTCTCTTGTCGAGTCTACAGGGACATACATTGCTGTATCGAGATATGCAAGTGCAACACCAATCCCACCTTCTGTGCTTAGTCGTGGAATCGGTTGGCAGGGATTTGGAATTCTTCTCTGTGGATTATTTGGAGCAGGAAATGCAACATCCGTGTCTGTAGAGAATGCCGGCTTGTTGGCTGTAACACGCGTTGGTAGTAGACGTGTAATTCAAGTAGCAGCTGGATTCATGATCTTCTTCTCCATTCTTGGTAAATTTGGGGCTATCTTTGCTTCGATTCCAGCTCCTATCGTCGCAGCTCTTTACTGTTTGTTCTTCTCATACGTTGGTGCTGGTGGTCTAAGTCTAATCCAGTTCTGCAATCTGAACAGTTTCAGAACTAAGTTCATTCTTGGATTCTCCATCTTCATGGGCTTATCCATACCTCAATACTTTTACCAGTACACAACTTTGGAAACCTATGGACCCGTTCGGACATCCGCAACATGGTTCAACAACATTATAAACGTTCCTTTCTCATCTAAAGCATTTGTTTCCGGGATTTTGGCCTTCTTTCTAGACACGACATTGCCTCCAAAGGACAAGACGACAAAGAAAGATCGAGGACTAGTTTGGTGGAAGCGATTTAAATCGTTCCAATCTGATAACAGAAGCGAAGAGTTCTACTCTTTGCCTCTCAATCTCAGCAAGTACTTTCCTTCTCACTGA

Protein Analysis

539

Amino Acids

59.51

Weight (kDa)

9.22

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 42 - 387 6e-53 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1415
AccI GTMKAC 2 cut(s) 926, 1111
AccII CGCG 1 cut(s) 1101
AciI CCGC 2 cut(s) 35, 1392
AclI AACGTT 1 cut(s) 1419
AclWI GGATC 3 cut(s) 81, 380, 393
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 2 cut(s) 1020, 1166
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 3 cut(s) 635, 1355, 1604
AfeI AGCGCT 1 cut(s) 814
AfiI CCNNNNNNNGG 2 cut(s) 744, 1398
AflIII ACRYGT 4 cut(s) 270, 662, 1099, 1114
AgsI TTSAA 8 cut(s) 146, 201, 281, 404, 848, 871, 1124, 1405
AhlI ACTAGT 1 cut(s) 1519
AjiI CACGTC 1 cut(s) 1115
AjuI GAANNNNNNNTTGG 2 cut(s) 964, 996
AloI GAACNNNNNNTCC 4 cut(s) 159, 191, 549, 581
AluBI AGCT 7 cut(s) 285, 320, 362, 860, 1133, 1196, 1211
AluI AGCT 7 cut(s) 285, 320, 362, 860, 1133, 1196, 1211
Alw21I GWGCWC 2 cut(s) 433, 862
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 3 cut(s) 81, 380, 393
Aor51HI AGCGCT 1 cut(s) 814
AoxI GGCC 3 cut(s) 119, 475, 1458
ApeKI GCWGC 4 cut(s) 362, 899, 1130, 1208
ApoI RAATTY 2 cut(s) 1020, 1166
Asp700I GAANNNNTTC 2 cut(s) 51, 1283
AspLEI GCGC 2 cut(s) 486, 815
AspS9I GGNCC 1 cut(s) 1376
AsuC2I CCSGG 1 cut(s) 1450
AsuHPI GGTGA 4 cut(s) 20, 79, 718, 830
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 1 cut(s) 1376
BamHI GGATCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 433, 862
BbvI GCAGC 4 cut(s) 349, 886, 1142, 1220
BccI CCATC 3 cut(s) 5, 889, 1322
BcnI CCSGG 1 cut(s) 1450
BcoDI GTCTC 1 cut(s) 119
BcuI ACTAGT 1 cut(s) 1519
BfaI CTAG 4 cut(s) 495, 575, 1469, 1520
BfmI CTRYAG 3 cut(s) 516, 927, 1068
BfoI RGCGCY 1 cut(s) 816
BglII AGATCT 1 cut(s) 49
BisI GCNGC 4 cut(s) 363, 900, 1131, 1209
BlsI GCNGC 4 cut(s) 364, 901, 1132, 1210
BmcAI AGTACT 1 cut(s) 1604
Bme1390I CCNGG 1 cut(s) 1450
Bme18I GGWCC 1 cut(s) 1376
BmgBI CACGTC 1 cut(s) 1115
BmgT120I GGNCC 1 cut(s) 1376
BmiI GGNNCC 2 cut(s) 387, 1378
BmrFI CCNGG 1 cut(s) 1450
BmsI GCATC 4 cut(s) 150, 199, 639, 813
BmtI GCTAGC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 293
BplI GAGNNNNNCTC 2 cut(s) 1562, 1594
BpuMI CCSGG 1 cut(s) 1450
BsaBI GATNNNNATC 1 cut(s) 1540
BsaJI CCNNGG 3 cut(s) 116, 424, 821
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 1468, 1498
Bsc4I CCNNNNNNNGG 2 cut(s) 744, 1398
Bse118I RCCGGY 1 cut(s) 1079
Bse1I ACTGG 2 cut(s) 1264, 1351
Bse3DI GCAATG 3 cut(s) 493, 939, 1478
Bse8I GATNNNNATC 1 cut(s) 1540
BseDI CCNNGG 3 cut(s) 116, 424, 821
BseGI GGATG 3 cut(s) 61, 1058, 1388
BseJI GATNNNNATC 1 cut(s) 1540
BseLI CCNNNNNNNGG 2 cut(s) 744, 1398
BseMI GCAATG 3 cut(s) 493, 939, 1478
BseMII CTCAG 3 cut(s) 594, 714, 1609
BseNI ACTGG 2 cut(s) 1264, 1351
BseRI GAGGAG 2 cut(s) 102, 870
BseXI GCAGC 4 cut(s) 349, 886, 1142, 1220
Bsh1236I CGCG 1 cut(s) 1101
Bsh1285I CGRYCG 1 cut(s) 796
BshFI GGCC 3 cut(s) 121, 477, 1460
BsiEI CGRYCG 1 cut(s) 796
BsiHKAI GWGCWC 2 cut(s) 433, 862
BsiSI CCGG 4 cut(s) 65, 122, 1080, 1449
BslFI GGGAC 3 cut(s) 307, 492, 946
BslI CCNNNNNNNGG 2 cut(s) 744, 1398
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 3 cut(s) 307, 492, 946
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 3 cut(s) 121, 477, 1460
Bsp1286I GDGCHC 2 cut(s) 433, 862
Bsp143I GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
Bsp19I CCATGG 1 cut(s) 821
BspACI CCGC 2 cut(s) 35, 1392
BspANI GGCC 3 cut(s) 121, 477, 1460
BspCNI CTCAG 3 cut(s) 595, 713, 1608
BspFNI CGCG 1 cut(s) 1101
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 387, 1378
BspMAI CTGCAG 1 cut(s) 520
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 3 cut(s) 81, 380, 393
BsrDI GCAATG 3 cut(s) 493, 939, 1478
BsrFI RCCGGY 1 cut(s) 1079
BsrI ACTGG 2 cut(s) 1264, 1351
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 3 cut(s) 116, 424, 821
BssMI GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
BssT1I CCWWGG 3 cut(s) 116, 424, 821
Bst4CI ACNGT 6 cut(s) 86, 175, 633, 797, 1220, 1283
Bst6I CTCTTC 1 cut(s) 1563
BstAPI GCANNNNNTGC 1 cut(s) 1052
BstC8I GCNNGC 3 cut(s) 576, 802, 1081
BstDEI CTNAG 8 cut(s) 154, 358, 603, 700, 989, 1253, 1293, 1595
BstDSI CCRYGG 1 cut(s) 821
BstF5I GGATG 3 cut(s) 61, 1058, 1388
BstFNI CGCG 1 cut(s) 1101
BstH2I RGCGCY 1 cut(s) 816
BstHHI GCGC 2 cut(s) 486, 815
BstKTI GATC 7 cut(s) 52, 76, 388, 411, 609, 1146, 1513
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
BstMCI CGRYCG 1 cut(s) 796
BstMWI GCNNNNNNNGC 5 cut(s) 483, 810, 896, 1052, 1181
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 1 cut(s) 1448
BstSFI CTRYAG 3 cut(s) 516, 927, 1068
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 4 cut(s) 349, 886, 1142, 1220
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 2 cut(s) 49, 385
BstXI CCANNNNNNTGG 1 cut(s) 1321
BstYI RGATCY 2 cut(s) 49, 385
BsuRI GGCC 3 cut(s) 121, 477, 1460
BtgI CCRYGG 1 cut(s) 821
BtrI CACGTC 1 cut(s) 1115
BtsCI GGATG 3 cut(s) 61, 1058, 1388
BtsIMutI CAGTG 3 cut(s) 478, 708, 1615
Cac8I GCNNGC 3 cut(s) 576, 802, 1081
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 2 cut(s) 486, 815
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 1 cut(s) 1376
Csp6I GTAC 3 cut(s) 634, 1354, 1603
CviAII CATG 6 cut(s) 412, 663, 822, 1141, 1321, 1398
CviQI GTAC 3 cut(s) 634, 1354, 1603
DdeI CTNAG 8 cut(s) 154, 358, 603, 700, 989, 1253, 1293, 1595
DpnI GATC 7 cut(s) 51, 75, 387, 410, 608, 1145, 1512
DpnII GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
DraI TTTAAA 1 cut(s) 1540
EaeI YGGCCR 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 1563
EarI CTCTTC 1 cut(s) 1563
Ecl136II GAGCTC 1 cut(s) 860
Eco130I CCWWGG 3 cut(s) 116, 424, 821
Eco24I GRGCYC 1 cut(s) 862
Eco47I GGWCC 1 cut(s) 1376
Eco47III AGCGCT 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 860
Eco57I CTGAAG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 1020
EcoT14I CCWWGG 3 cut(s) 116, 424, 821
EcoT22I ATGCAT 2 cut(s) 587, 727
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 3 cut(s) 116, 424, 821
FaeI CATG 6 cut(s) 415, 666, 825, 1144, 1324, 1401
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 3 cut(s) 307, 492, 946
FatI CATG 6 cut(s) 411, 662, 821, 1140, 1320, 1397
FblI GTMKAC 2 cut(s) 926, 1111
Fnu4HI GCNGC 4 cut(s) 363, 900, 1131, 1209
FokI GGATG 3 cut(s) 48, 1045, 1375
FriOI GRGCYC 1 cut(s) 862
Fsp4HI GCNGC 4 cut(s) 363, 900, 1131, 1209
FspBI CTAG 4 cut(s) 495, 575, 1469, 1520
GlaI GCGC 2 cut(s) 485, 814
GluI GCNGC 4 cut(s) 363, 900, 1131, 1209
HaeII RGCGCY 1 cut(s) 816
HaeIII GGCC 3 cut(s) 121, 477, 1460
HapII CCGG 4 cut(s) 65, 122, 1080, 1449
HhaI GCGC 2 cut(s) 486, 815
Hin1II CATG 6 cut(s) 415, 666, 825, 1144, 1324, 1401
Hin6I GCGC 2 cut(s) 484, 813
HinP1I GCGC 2 cut(s) 484, 813
HindIII AAGCTT 1 cut(s) 283
HpaII CCGG 4 cut(s) 65, 122, 1080, 1449
HphI GGTGA 4 cut(s) 20, 79, 718, 830
Hpy166II GTNNAC 3 cut(s) 927, 1112, 1356
Hpy188I TCNGA 6 cut(s) 243, 648, 1278, 1289, 1386, 1555
Hpy188III TCNNGA 4 cut(s) 261, 951, 1141, 1469
Hpy8I GTNNAC 3 cut(s) 927, 1112, 1356
Hpy99I CGWCG 1 cut(s) 1208
HpyAV CCTTC 4 cut(s) 64, 990, 1471, 1620
HpyCH4III ACNGT 6 cut(s) 86, 175, 633, 797, 1220, 1283
HpyCH4IV ACGT 5 cut(s) 245, 272, 1114, 1236, 1419
HpyF10VI GCNNNNNNNGC 5 cut(s) 483, 810, 896, 1052, 1181
HpyF3I CTNAG 8 cut(s) 154, 358, 603, 700, 989, 1253, 1293, 1595
HpySE526I ACGT 5 cut(s) 245, 272, 1114, 1236, 1419
Hsp92II CATG 6 cut(s) 415, 666, 825, 1144, 1324, 1401
HspAI GCGC 2 cut(s) 484, 813
KroI GCCGGC 1 cut(s) 1079
KroNI GCCGGC 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
LmnI GCTCC 5 cut(s) 317, 857, 865, 1043, 1201
Lsp1109I GCAGC 4 cut(s) 349, 886, 1142, 1220
LweI GCATC 4 cut(s) 150, 199, 639, 813
MaeI CTAG 4 cut(s) 495, 575, 1469, 1520
MaeII ACGT 5 cut(s) 245, 272, 1114, 1236, 1419
MaeIII GTNAC 4 cut(s) 368, 490, 755, 1093
MalI GATC 7 cut(s) 51, 75, 387, 410, 608, 1145, 1512
MboI GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
MflI RGATCY 2 cut(s) 49, 385
MhlI GDGCHC 2 cut(s) 433, 862
MluCI AATT 3 cut(s) 1020, 1119, 1166
MluI ACGCGT 1 cut(s) 1099
MlyI GAGTC 4 cut(s) 289, 514, 543, 932
MmeI TCCRAC 2 cut(s) 209, 887
Mph1103I ATGCAT 2 cut(s) 587, 727
MroNI GCCGGC 1 cut(s) 1079
MroXI GAANNNNTTC 2 cut(s) 51, 1283
MseI TTAA 1 cut(s) 1539
MslI CAYNNNNRTG 1 cut(s) 1319
MspA1I CMGCKG 1 cut(s) 1133
MspI CCGG 4 cut(s) 65, 122, 1080, 1449
MspR9I CCNGG 1 cut(s) 1450
Mva1269I GAATGC 1 cut(s) 1081
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 5 cut(s) 483, 810, 896, 1052, 1181
NaeI GCCGGC 1 cut(s) 1081
NciI CCSGG 1 cut(s) 1450
NcoI CCATGG 1 cut(s) 821
NdeII GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
NgoMIV GCCGGC 1 cut(s) 1079
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 6 cut(s) 415, 666, 825, 1144, 1324, 1401
NlaIV GGNNCC 2 cut(s) 387, 1378
NsiI ATGCAT 2 cut(s) 587, 727
NspI RCATGY 1 cut(s) 666
PagI TCATGA 1 cut(s) 1140
PciI ACATGT 1 cut(s) 662
PctI GAATGC 1 cut(s) 1081
PdiI GCCGGC 1 cut(s) 1081
PdmI GAANNNNTTC 2 cut(s) 51, 1283
PfeI GAWTC 8 cut(s) 238, 264, 420, 564, 999, 1137, 1189, 1308
PfoI TCCNGGA 1 cut(s) 1448
PkrI GCNGC 4 cut(s) 364, 901, 1132, 1210
PleI GAGTC 4 cut(s) 289, 513, 543, 931
PpsI GAGTC 4 cut(s) 289, 513, 543, 931
PscI ACATGT 1 cut(s) 662
PsiI TTATAA 1 cut(s) 1415
Psp124BI GAGCTC 1 cut(s) 862
Psp1406I AACGTT 1 cut(s) 1419
PspN4I GGNNCC 2 cut(s) 387, 1378
PspPI GGNCC 1 cut(s) 1376
PstI CTGCAG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 49, 385
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 3 cut(s) 635, 1355, 1604
RsaNI GTAC 3 cut(s) 634, 1354, 1603
RseI CAYNNNNRTG 1 cut(s) 1319
SacI GAGCTC 1 cut(s) 862
SaqAI TTAA 1 cut(s) 1539
SatI GCNGC 4 cut(s) 363, 900, 1131, 1209
Sau3AI GATC 7 cut(s) 49, 73, 385, 408, 606, 1143, 1510
Sau96I GGNCC 1 cut(s) 1376
ScaI AGTACT 1 cut(s) 1604
SchI GAGTC 4 cut(s) 289, 514, 543, 932
ScrFI CCNGG 1 cut(s) 1450
SduI GDGCHC 2 cut(s) 433, 862
SfaNI GCATC 4 cut(s) 150, 199, 639, 813
SfcI CTRYAG 3 cut(s) 516, 927, 1068
SinI GGWCC 1 cut(s) 1376
SmiI ATTTAAAT 1 cut(s) 1540
SmiMI CAYNNNNRTG 1 cut(s) 1319
SpeI ACTAGT 1 cut(s) 1519
Sse9I AATT 3 cut(s) 1020, 1119, 1166
SsiI CCGC 2 cut(s) 35, 1392
SspMI CTAG 4 cut(s) 495, 575, 1469, 1520
SstI GAGCTC 1 cut(s) 862
StyD4I CCNGG 1 cut(s) 1448
StyI CCWWGG 3 cut(s) 116, 424, 821
SwaI ATTTAAAT 1 cut(s) 1540
TaaI ACNGT 6 cut(s) 86, 175, 633, 797, 1220, 1283
TaiI ACGT 5 cut(s) 248, 275, 1117, 1239, 1422
TaqI TCGA 4 cut(s) 921, 950, 1187, 1513
TasI AATT 3 cut(s) 1020, 1119, 1166
TatI WGTACW 3 cut(s) 633, 1353, 1602
TfiI GAWTC 8 cut(s) 238, 264, 420, 564, 999, 1137, 1189, 1308
Tru1I TTAA 1 cut(s) 1539
Tru9I TTAA 1 cut(s) 1539
TscAI CASTG 2 cut(s) 478, 708
TseI GCWGC 4 cut(s) 362, 899, 1130, 1208
TspDTI ATGAA 5 cut(s) 443, 570, 1129, 1288, 1309
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 2 cut(s) 478, 708
VpaK11BI GGWCC 1 cut(s) 1376
XapI RAATTY 2 cut(s) 1020, 1166
XbaI TCTAGA 1 cut(s) 1468
XceI RCATGY 1 cut(s) 666
XmiI GTMKAC 2 cut(s) 926, 1111
XmnI GAANNNNTTC 2 cut(s) 51, 1283
XspI CTAG 4 cut(s) 495, 575, 1469, 1520
ZrmI AGTACT 1 cut(s) 1604
Zsp2I ATGCAT 2 cut(s) 587, 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.