MD13G1087500.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005215
Physical Location & Seq
Reverse (-)
6133582 .. 6138422
4841 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1087500.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGGCAGGAGGTGGACATGCACCGCCACCGAAACAGGAGGAGCTACAGCCTCATCTGGTCAAAGATCAGCTGCCTAACGTTTACTACTGCATTACCAGTCCTCCTCCTTGGCCTGAAGCCATCCTACTTGGTTTCCAACATTACTTGGTGATGCTTGGCACGACGGTTCTGATTCCCACCTCTCTCGTTCCTCAGATGGGAGGAGGAAGCGGGGAGAAAGCAAAAATGATTCAGACTATACTCTTTGTTGCTGGATTAAACACACTGCTTCAAACATTCTTTGGGACTCGTTTACCTGCAGTGATTGGAGCATCCTATACCTATGTGCCAACAACCATTTCGATTATCTTGGCTGGTCGGTATAGTGACATTGTGAACCCCCAGGAGAAATTTGAGAAGATAATGCGTGGAACCCAGGGTGCACTTATTGTTGCCTCAACTCTGCAGATTGTAGTTGGCTTCAGCGGTCTTTGGCGCAATGTGGCGAGGTTCCTAAGTCCACTTTCTGCTGTTCCTTTGGTCGCTTTGTCAGGATTTGGGCTATACGAGTTTGGTTTTCCTGTGTTTGCGAAGTGTGTGGAGATTGGGCTACCGCAACTGATCATGCTACTGATATTTTCACAGTACATCCCTCATTTGTTGCGCAGTGAGACGCATGTCTTTGATCGCTTTGCTGTTCTATTTTCGGTGGTCATTGTGTGGATTTATGCTCATCTGCTTACTGTGGGTGGGGCCTATAAGAACACAGGACCCAGAACTCAATTAACCTGTCGAACTGATCGTGCTGGTATCATAGGTGCTGCTCCATGGATAAGGATTCCATATCCCTTTCAATGGGGAGCTCCCACATTCGATGCTGGAGAAGCTTTTGCAATGATGGCTGTTTCATTTGTTGCTCTTGTAGAGTCCACCGGTGCCTTTATTGCTGTGTCAAGGTATGCAAGTGCAACTCCGCTGCCACCTTCTGTTTTAAGCCGCGGTGTTGGTTGGCAGGGAGTCGGAATTCTTTTCTGTGGTATATTCGGCACTGGGAATGGAGCTTCAGTATCTGTTGAAAATGCTGGTTTGTTAGCATTGACACGGGTTGGTAGCCGAAGGGTGGTTCAAATTTCAGCCTGTTTCATGATCTTCTTTTCCATACTTGGAAAATTTGGAGCTGTCTTTGCCTCAATTCCAGCACCCATCATTGCAGCATTGTATTGCTTTTTCTTCGCTTATGTCGGTTCTGCAGGTCTTACCTTGCTTCAATTTTGCAACCTCAACAGTTTTCGGACGAAATTTATCTTAGGCTTCTCTATTTACATGGGCTTGTCGATTCCACAATATTTCAACGAATACACCCTCATTAGAGGCTACGGCCCCGTGCACACCGGAGCAAGATGGTTCAATGATATGATCAACGTTCCTTTCTCGTCCGAACCATTTGTGGCCGGTTTCTTGGCAGTATTCTTGGACATCACGCTACACCGCAAGGACAGCGCTACAAGGAAAGATCGCGGCATGCATTGGTGGGATAGATTCCGATCTTTCAAGACAGATACAAGAAGCGAGGAATTCTACTCCCTGCCTTTCAACCTCAACAAGTTTTTCCCCTCAGTGTGA

Protein Analysis

534

Amino Acids

58.51

Weight (kDa)

9.36

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 443 3.3e-64 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 644
Acc36I ACCTGC 2 cut(s) 304, 1220
AccB1I GGYRCC 1 cut(s) 914
AccII CGCG 2 cut(s) 978, 1497
AciI CCGC 9 cut(s) 23, 210, 465, 593, 953, 976, 978, 1468, 1497
AclI AACGTT 2 cut(s) 78, 1401
AcoI YGGCCR 1 cut(s) 1428
AcsI RAATTY 6 cut(s) 389, 1002, 1107, 1148, 1277, 1553
AcuI CTGAAG 3 cut(s) 135, 445, 1026
AfaI GTAC 1 cut(s) 626
AfeI AGCGCT 1 cut(s) 1480
AfiI CCNNNNNNNGG 3 cut(s) 197, 834, 1099
AgeI ACCGGT 1 cut(s) 911
AgsI TTSAA 9 cut(s) 272, 833, 1055, 1106, 1247, 1330, 1387, 1531, 1573
AjnI CCWGG 2 cut(s) 381, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 6 cut(s) 43, 70, 842, 866, 1040, 1157
AluI AGCT 6 cut(s) 43, 70, 842, 866, 1040, 1157
Alw21I GWGCWC 3 cut(s) 424, 844, 1368
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 2 cut(s) 420, 1364
AlwNI CAGNNNCTG 1 cut(s) 1049
Aor51HI AGCGCT 1 cut(s) 1480
AoxI GGCC 4 cut(s) 110, 732, 1357, 1428
ApaLI GTGCAC 2 cut(s) 420, 1364
ApeKI GCWGC 4 cut(s) 70, 800, 955, 1190
ApoI RAATTY 6 cut(s) 389, 1002, 1107, 1148, 1277, 1553
AsiGI ACCGGT 1 cut(s) 911
AspLEI GCGC 3 cut(s) 477, 645, 1481
AspS9I GGNCC 3 cut(s) 732, 749, 1358
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 2 cut(s) 424, 1368
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 844
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
Bbv12I GWGCWC 3 cut(s) 424, 844, 1368
BbvI GCAGC 4 cut(s) 57, 787, 942, 1202
BccI CCATC 5 cut(s) 128, 190, 871, 1190, 1374
BceAI ACGGC 1 cut(s) 1372
BciT130I CCWGG 2 cut(s) 383, 416
BclI TGATCA 2 cut(s) 600, 1395
BcoDI GTCTC 1 cut(s) 644
BfmI CTRYAG 4 cut(s) 44, 297, 443, 1227
BfoI RGCGCY 1 cut(s) 1482
BfuAI ACCTGC 2 cut(s) 304, 1220
BisI GCNGC 6 cut(s) 71, 801, 956, 976, 1191, 1498
BlsI GCNGC 6 cut(s) 72, 802, 957, 977, 1192, 1499
Bme1390I CCNGG 2 cut(s) 383, 416
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 3 cut(s) 732, 749, 1358
BmiI GGNNCC 6 cut(s) 412, 491, 733, 751, 916, 1360
BmrFI CCNGG 2 cut(s) 383, 416
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 3 cut(s) 141, 320, 844
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 656
BpmI CTGGAG 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 1522
BsaJI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 976
BsaWI WCCGGW 2 cut(s) 911, 1370
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 1029, 1059
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 834, 1099
Bse118I RCCGGY 2 cut(s) 911, 1430
Bse1I ACTGG 2 cut(s) 96, 1033
Bse3DI GCAATG 3 cut(s) 484, 879, 1185
Bse8I GATNNNNATC 1 cut(s) 1522
BseBI CCWGG 2 cut(s) 383, 416
BseDI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 976
BseGI GGATG 3 cut(s) 120, 311, 627
BseJI GATNNNNATC 1 cut(s) 1522
BseLI CCNNNNNNNGG 3 cut(s) 197, 834, 1099
BseMI GCAATG 3 cut(s) 484, 879, 1185
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 2 cut(s) 96, 1033
BseRI GAGGAG 3 cut(s) 53, 93, 216
BseSI GKGCMC 2 cut(s) 424, 1368
BseXI GCAGC 4 cut(s) 57, 787, 942, 1202
Bsh1236I CGCG 2 cut(s) 978, 1497
BshFI GGCC 4 cut(s) 112, 734, 1359, 1430
BshNI GGYRCC 1 cut(s) 914
BshTI ACCGGT 1 cut(s) 911
BsiHKAI GWGCWC 3 cut(s) 424, 844, 1368
BsiSI CCGG 3 cut(s) 912, 1371, 1431
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 197, 834, 1099
BsmAI GTCTC 1 cut(s) 644
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 4 cut(s) 112, 734, 1359, 1430
Bsp1286I GDGCHC 3 cut(s) 424, 844, 1368
Bsp143I GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 9 cut(s) 23, 210, 465, 593, 953, 976, 978, 1468, 1497
BspANI GGCC 4 cut(s) 112, 734, 1359, 1430
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 2 cut(s) 978, 1497
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 6 cut(s) 412, 491, 733, 751, 916, 1360
BspMAI CTGCAG 3 cut(s) 301, 447, 1231
BspMI ACCTGC 2 cut(s) 304, 1220
BspT107I GGYRCC 1 cut(s) 914
BsrDI GCAATG 3 cut(s) 484, 879, 1185
BsrFI RCCGGY 2 cut(s) 911, 1430
BsrI ACTGG 2 cut(s) 96, 1033
BssAI RCCGGY 2 cut(s) 911, 1430
BssECI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 976
BssMI GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
BssT1I CCWWGG 2 cut(s) 107, 806
Bst2UI CCWGG 2 cut(s) 383, 416
Bst4CI ACNGT 4 cut(s) 166, 624, 724, 1265
BstC8I GCNNGC 1 cut(s) 1502
BstDEI CTNAG 4 cut(s) 192, 494, 1285, 1594
BstDSI CCRYGG 2 cut(s) 806, 976
BstF5I GGATG 3 cut(s) 120, 311, 627
BstFNI CGCG 2 cut(s) 978, 1497
BstH2I RGCGCY 1 cut(s) 1482
BstHHI GCGC 3 cut(s) 477, 645, 1481
BstKTI GATC 8 cut(s) 67, 603, 667, 781, 1128, 1398, 1495, 1526
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
BstMWI GCNNNNNNNGC 5 cut(s) 863, 878, 923, 1163, 1476
BstNI CCWGG 2 cut(s) 383, 416
BstNSI RCATGY 3 cut(s) 20, 659, 1504
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 381, 414
BstSFI CTRYAG 4 cut(s) 44, 297, 443, 1227
BstSLI GKGCMC 2 cut(s) 424, 1368
BstUI CGCG 2 cut(s) 978, 1497
BstV1I GCAGC 4 cut(s) 57, 787, 942, 1202
BsuRI GGCC 4 cut(s) 112, 734, 1359, 1430
BtgI CCRYGG 2 cut(s) 806, 976
BtsCI GGATG 3 cut(s) 120, 311, 627
BtsI GCAGTG 3 cut(s) 263, 306, 652
BtsIMutI CAGTG 5 cut(s) 263, 306, 652, 1026, 1602
BveI ACCTGC 2 cut(s) 304, 1220
Cac8I GCNNGC 1 cut(s) 1502
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 3 cut(s) 477, 645, 1481
Cfr10I RCCGGY 2 cut(s) 911, 1430
Cfr13I GGNCC 3 cut(s) 732, 749, 1358
Cfr42I CCGCGG 1 cut(s) 979
CseI GACGC 1 cut(s) 661
Csp6I GTAC 1 cut(s) 625
CspAI ACCGGT 1 cut(s) 911
CviAII CATG 7 cut(s) 17, 604, 656, 807, 1123, 1303, 1501
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 4 cut(s) 192, 494, 1285, 1594
DpnI GATC 8 cut(s) 66, 602, 666, 780, 1127, 1397, 1494, 1525
DpnII GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
EaeI YGGCCR 1 cut(s) 1428
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 2 cut(s) 107, 806
Eco24I GRGCYC 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 749
Eco47III AGCGCT 1 cut(s) 1480
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 3 cut(s) 135, 445, 1026
EcoICRI GAGCTC 1 cut(s) 842
EcoO109I RGGNCCY 2 cut(s) 732, 749
EcoRI GAATTC 2 cut(s) 1002, 1553
EcoRII CCWGG 2 cut(s) 381, 414
EcoT14I CCWWGG 2 cut(s) 107, 806
EcoT22I ATGCAT 1 cut(s) 1506
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 2 cut(s) 107, 806
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 7 cut(s) 20, 607, 659, 810, 1126, 1306, 1504
FaqI GGGAC 1 cut(s) 298
FatI CATG 7 cut(s) 16, 603, 655, 806, 1122, 1302, 1500
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 2 cut(s) 600, 1395
Fnu4HI GCNGC 6 cut(s) 71, 801, 956, 976, 1191, 1498
FokI GGATG 3 cut(s) 107, 298, 614
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 6 cut(s) 71, 801, 956, 976, 1191, 1498
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 3 cut(s) 476, 644, 1480
GluI GCNGC 6 cut(s) 71, 801, 956, 976, 1191, 1498
GsuI CTGGAG 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 1482
HaeIII GGCC 4 cut(s) 112, 734, 1359, 1430
HapII CCGG 3 cut(s) 912, 1371, 1431
HgaI GACGC 1 cut(s) 661
HhaI GCGC 3 cut(s) 477, 645, 1481
Hin1II CATG 7 cut(s) 20, 607, 659, 810, 1126, 1306, 1504
Hin6I GCGC 3 cut(s) 475, 643, 1479
HinP1I GCGC 3 cut(s) 475, 643, 1479
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 8 cut(s) 172, 229, 286, 817, 905, 996, 1315, 1518
HpaII CCGG 3 cut(s) 912, 1371, 1431
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 8 cut(s) 14, 82, 293, 376, 422, 500, 909, 1366
Hpy188I TCNGA 7 cut(s) 171, 195, 234, 1001, 1272, 1417, 1523
Hpy188III TCNNGA 3 cut(s) 531, 1123, 1531
Hpy8I GTNNAC 8 cut(s) 14, 82, 293, 376, 422, 500, 909, 1366
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 972, 1089
HpyCH4III ACNGT 4 cut(s) 166, 624, 724, 1265
HpyCH4IV ACGT 2 cut(s) 78, 1401
HpyF10VI GCNNNNNNNGC 5 cut(s) 863, 878, 923, 1163, 1476
HpyF3I CTNAG 4 cut(s) 192, 494, 1285, 1594
HpySE526I ACGT 2 cut(s) 78, 1401
Hsp92II CATG 7 cut(s) 20, 607, 659, 810, 1126, 1306, 1504
HspAI GCGC 3 cut(s) 475, 643, 1479
Ksp22I TGATCA 2 cut(s) 600, 1395
KspI CCGCGG 1 cut(s) 979
Kzo9I GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
LmnI GCTCC 8 cut(s) 40, 308, 808, 839, 847, 1037, 1154, 1373
Lsp1109I GCAGC 4 cut(s) 57, 787, 942, 1202
LweI GCATC 3 cut(s) 141, 320, 844
MaeII ACGT 2 cut(s) 78, 1401
MaeIII GTNAC 1 cut(s) 365
MalI GATC 8 cut(s) 66, 602, 666, 780, 1127, 1397, 1494, 1525
MboI GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
MboII GAAGA 3 cut(s) 409, 1120, 1201
MhlI GDGCHC 3 cut(s) 424, 844, 1368
MluCI AATT 9 cut(s) 389, 761, 1002, 1107, 1148, 1170, 1247, 1277, 1553
MlyI GAGTC 3 cut(s) 280, 914, 1005
MmeI TCCRAC 2 cut(s) 160, 979
Mph1103I ATGCAT 1 cut(s) 1506
MseI TTAA 3 cut(s) 257, 764, 971
MspA1I CMGCKG 4 cut(s) 70, 465, 955, 978
MspI CCGG 3 cut(s) 912, 1371, 1431
MspR9I CCNGG 2 cut(s) 383, 416
MvaI CCWGG 2 cut(s) 383, 416
MvnI CGCG 2 cut(s) 978, 1497
MwoI GCNNNNNNNGC 5 cut(s) 863, 878, 923, 1163, 1476
NcoI CCATGG 1 cut(s) 806
NdeII GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
NlaIII CATG 7 cut(s) 20, 607, 659, 810, 1126, 1306, 1504
NlaIV GGNNCC 6 cut(s) 412, 491, 733, 751, 916, 1360
NmuCI GTSAC 1 cut(s) 365
NsbI TGCGCA 1 cut(s) 644
NsiI ATGCAT 1 cut(s) 1506
NspI RCATGY 3 cut(s) 20, 659, 1504
PaeI GCATGC 1 cut(s) 1504
PagI TCATGA 1 cut(s) 1122
PasI CCCWGGG 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PfeI GAWTC 5 cut(s) 172, 229, 817, 1315, 1518
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 6 cut(s) 72, 802, 957, 977, 1192, 1499
PleI GAGTC 3 cut(s) 280, 913, 1004
PpsI GAGTC 3 cut(s) 280, 913, 1004
PpuMI RGGWCCY 1 cut(s) 749
PshAI GACNNNNGTC 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 844
Psp1406I AACGTT 2 cut(s) 78, 1401
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 2 cut(s) 381, 414
PspGI CCWGG 2 cut(s) 381, 414
PspN4I GGNNCC 6 cut(s) 412, 491, 733, 751, 916, 1360
PspPI GGNCC 3 cut(s) 732, 749, 1358
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 3 cut(s) 301, 447, 1231
PstNI CAGNNNCTG 1 cut(s) 1049
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
SacI GAGCTC 1 cut(s) 844
SacII CCGCGG 1 cut(s) 979
SaqAI TTAA 3 cut(s) 257, 764, 971
SatI GCNGC 6 cut(s) 71, 801, 956, 976, 1191, 1498
Sau3AI GATC 8 cut(s) 64, 600, 664, 778, 1125, 1395, 1492, 1523
Sau96I GGNCC 3 cut(s) 732, 749, 1358
SchI GAGTC 3 cut(s) 280, 914, 1005
ScrFI CCNGG 2 cut(s) 383, 416
SduI GDGCHC 3 cut(s) 424, 844, 1368
SfaNI GCATC 3 cut(s) 141, 320, 844
SfcI CTRYAG 4 cut(s) 44, 297, 443, 1227
Sfr303I CCGCGG 1 cut(s) 979
SgrAI CRCCGGYG 1 cut(s) 911
SgrBI CCGCGG 1 cut(s) 979
SinI GGWCC 1 cut(s) 749
SphI GCATGC 1 cut(s) 1504
Sse9I AATT 9 cut(s) 389, 761, 1002, 1107, 1148, 1170, 1247, 1277, 1553
SsiI CCGC 9 cut(s) 23, 210, 465, 593, 953, 976, 978, 1468, 1497
SspI AATATT 1 cut(s) 1325
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 381, 414
StyI CCWWGG 2 cut(s) 107, 806
TaaI ACNGT 4 cut(s) 166, 624, 724, 1265
TaiI ACGT 2 cut(s) 81, 1404
TaqI TCGA 4 cut(s) 341, 772, 852, 1313
TasI AATT 9 cut(s) 389, 761, 1002, 1107, 1148, 1170, 1247, 1277, 1553
TatI WGTACW 1 cut(s) 624
TauI GCSGC 2 cut(s) 978, 1500
TfiI GAWTC 5 cut(s) 172, 229, 817, 1315, 1518
Tru1I TTAA 3 cut(s) 257, 764, 971
Tru9I TTAA 3 cut(s) 257, 764, 971
TscAI CASTG 5 cut(s) 270, 306, 652, 1033, 1602
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 4 cut(s) 70, 800, 955, 1190
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 2 cut(s) 876, 1111
TspRI CASTG 5 cut(s) 270, 306, 652, 1033, 1602
VneI GTGCAC 2 cut(s) 420, 1364
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 6 cut(s) 389, 1002, 1107, 1148, 1277, 1553
XceI RCATGY 3 cut(s) 20, 659, 1504
Zsp2I ATGCAT 1 cut(s) 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.