Rroxscaffold_5G00384920

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005215
Physical Location & Seq
Forward (+)
64130789 .. 64135307
4519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384920.1

Sequence Viewer

Length: 1602 bp
ATGGCAGGAGGTGGACATGCACCACCACCGAAACAAGAGGAGCTACAGCCTCATCCAGTAAAAGATCAGCTACCAAACATTTCCTACTGCATTACCAGTCCTCCTCCTTGGCCTGAGGCTATCCTACTTGGTTTCCAACATTACTTGGTGATGCTTGGCACAGCAGTTCTCATTCCCACCTATCTTGTTCCTCAGATGGGAGGAGGAAATGAGGAGAAAGCAAAAATGATCCAGACTATACTGTTTGTTGCTGGAATCAACACATTGCAGCAGACATTTTTTGGTACTCGTTTACCTGCAGTAATTGGAGCATCTTATACCTATGTCCCAACAACCATTTCTATTATAATGGCTGGTCGATATAGTGATGTTGTGAATCCCCAGGAGAAATTTGAGAAAATAATGCGTGGAACCCAGGGTGCACTCATTGTTGCCTCTATTTTGCAGATTGTTGTTGGTTTCAGTGGCCTTTGGCGCAATGTGGTGAGGTTCCAAAGTCCACTTTCTACTGTTCCTTTGGTTGCTTTGTCAGGCTTTGGGCTATATGAGCTTGGTTTTCCTGTGTTTGCAAAATGTGTGGAGATTGGGCTACCACAACTCATCCTTCTACTGATATTTTCACAGTACATACCTCATCTGATGAGCAGTGAGACACATGTCTTTGATCGCTTTGCTGTTTTATTTTCGGTGACAATTGTGTGGATTTATGCTCATCTGCTCACTGTGGGCGGGGCTTATAAGAACACACCAGAAGCAACTCAGTTAACCTGTCGAACTGATCGTGCTGGCATCATACATGCTGCTCCATGGATAAGAGTTCCATATCCTTTCCAATGGGGAGCTCCCACTTTTGATGCTGGAGAAGCTTTTGCAATGATGACTGCTTCATTTGTTGCTCTTGTAGAGTCCACCGGTGTCTTTATTGCTGTGTCGAGGTATGCAAGTGCAACTCCACTCCCACCTGCTATTTTAAGTCGCGGTATTGGTTGGCAGGGAGTGGGCATTCTTTTCTCTGGTATATTCGGCACTGGGAATGGATCATCAGTATCTGTTGAAAATGCTGGTTTGTTAGCTTTGACTCGAGTTGGTAGCCGAAGGGTTGTCCAAATTTCAGCCAGCTTCATGATCTTCTTTTCCATACTTGGAAAATTTGGAGCTGTCTTTGCCTCGATACCAGCACCCATTGTTGCAGCAATGTATTGCTTCTTCTTCGCTTACGTTGGCTCAGCAGGTCTTAGCTTGCTTCAGTTTTGCAATCTAAACAGTTTCAGGACTAAATTTATCATAGGCTTCTCAATTTTCATGGGCCTTTCAATACCACAATATTTCAATGAATATACCCTTGTTAAAGGCTATGGTCCGGTGCACACAGGAGCAAGATGGTTCAATGACATGATCAATGTTCCTTTTGCATCGGAGCCATTTGTTGCTGGTTTATTGGCATTTGTTCTGGACACCACACTGCATCGAAAGGACAGTGCGACGAGGAAAGACAGGGGCTTGCATTGGTGGGACAGATTCCGATCATACAAGACAGATACAAGAAGTGAGGAGTTCTATTCCTTGCCTTTCAACCTCAACAAGTTTTTCCCTTCAGTGTAA

Protein Analysis

533

Amino Acids

58.53

Weight (kDa)

9.14

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 443 5.4e-65 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 347, 738
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 3 cut(s) 304, 970, 1220
AccII CGCG 1 cut(s) 978
AciI CCGC 2 cut(s) 729, 978
AclWI GGATC 2 cut(s) 223, 1045
AcsI RAATTY 4 cut(s) 389, 1107, 1148, 1277
AcuI CTGAAG 2 cut(s) 1229, 1578
AfaI GTAC 2 cut(s) 286, 626
AfiI CCNNNNNNNGG 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 655
AgeI ACCGGT 1 cut(s) 911
AgsI TTSAA 5 cut(s) 1055, 1314, 1330, 1387, 1573
AjnI CCWGG 2 cut(s) 381, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 9 cut(s) 43, 70, 550, 842, 866, 1073, 1119, 1157, 1239
AluI AGCT 9 cut(s) 43, 70, 550, 842, 866, 1073, 1119, 1157, 1239
Alw21I GWGCWC 3 cut(s) 424, 844, 1368
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 2 cut(s) 420, 1364
AlwI GGATC 2 cut(s) 223, 1045
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 1 cut(s) 1080
AoxI GGCC 3 cut(s) 110, 466, 1306
ApaLI GTGCAC 2 cut(s) 420, 1364
ApeKI GCWGC 3 cut(s) 268, 800, 1190
ApoI RAATTY 4 cut(s) 389, 1107, 1148, 1277
AsiGI ACCGGT 1 cut(s) 911
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 2 cut(s) 1306, 1358
AsuHPI GGTGA 3 cut(s) 160, 496, 700
AvaI CYCGRG 1 cut(s) 1080
AvaII GGWCC 1 cut(s) 1358
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 424, 1368
BanII GRGCYC 1 cut(s) 844
BarI GAAGNNNNNNTAC 2 cut(s) 1190, 1222
Bbv12I GWGCWC 3 cut(s) 424, 844, 1368
BbvI GCAGC 3 cut(s) 280, 787, 1202
BccI CCATC 2 cut(s) 190, 1374
BciT130I CCWGG 2 cut(s) 383, 416
BclI TGATCA 1 cut(s) 1395
BcoDI GTCTC 1 cut(s) 644
BfmI CTRYAG 2 cut(s) 44, 297
BfuAI ACCTGC 3 cut(s) 304, 970, 1220
BisI GCNGC 3 cut(s) 269, 801, 1191
BlpI GCTNAGC 1 cut(s) 1225
BlsI GCNGC 3 cut(s) 270, 802, 1192
Bme1390I CCNGG 2 cut(s) 383, 416
Bme18I GGWCC 1 cut(s) 1358
BmeT110I CYCGRG 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 1306, 1358
BmiI GGNNCC 3 cut(s) 412, 491, 1419
BmrFI CCNGG 2 cut(s) 383, 416
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 6 cut(s) 141, 320, 798, 844, 1421, 1474
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 656
BpmI CTGGAG 1 cut(s) 879
Bpu1102I GCTNAGC 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 1522
BsaJI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BsaWI WCCGGW 2 cut(s) 911, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 3 cut(s) 56, 96, 1033
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 4 cut(s) 263, 484, 879, 1200
Bse8I GATNNNNATC 1 cut(s) 1522
BseBI CCWGG 2 cut(s) 383, 416
BseDI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BseGI GGATG 2 cut(s) 52, 600
BseJI GATNNNNATC 1 cut(s) 1522
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 4 cut(s) 263, 484, 879, 1200
BseMII CTCAG 4 cut(s) 105, 206, 773, 1239
BseNI ACTGG 3 cut(s) 56, 96, 1033
BseRI GAGGAG 5 cut(s) 53, 93, 216, 227, 1565
BseSI GKGCMC 2 cut(s) 424, 1368
BseXI GCAGC 3 cut(s) 280, 787, 1202
Bsh1236I CGCG 1 cut(s) 978
BshFI GGCC 3 cut(s) 112, 468, 1308
BshTI ACCGGT 1 cut(s) 911
BsiHKAI GWGCWC 3 cut(s) 424, 844, 1368
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 2 cut(s) 912, 1361
BslFI GGGAC 2 cut(s) 311, 1526
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 644
BsmFI GGGAC 2 cut(s) 311, 1526
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 3 cut(s) 112, 468, 1308
BsoBI CYCGRG 1 cut(s) 1080
Bsp1286I GDGCHC 3 cut(s) 424, 844, 1368
Bsp143I GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Bsp1720I GCTNAGC 1 cut(s) 1225
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 2 cut(s) 729, 978
BspANI GGCC 3 cut(s) 112, 468, 1308
BspCNI CTCAG 4 cut(s) 106, 205, 772, 1238
BspFNI CGCG 1 cut(s) 978
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 3 cut(s) 412, 491, 1419
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 3 cut(s) 304, 970, 1220
BspPI GGATC 2 cut(s) 223, 1045
BsrDI GCAATG 4 cut(s) 263, 484, 879, 1200
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 3 cut(s) 56, 96, 1033
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BssMI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
BssT1I CCWWGG 2 cut(s) 107, 806
Bst2UI CCWGG 2 cut(s) 383, 416
Bst4CI ACNGT 6 cut(s) 243, 511, 624, 724, 1265, 1478
BstC8I GCNNGC 4 cut(s) 787, 1117, 1241, 1502
BstDEI CTNAG 5 cut(s) 114, 192, 759, 1225, 1235
BstDSI CCRYGG 1 cut(s) 806
BstF5I GGATG 2 cut(s) 52, 600
BstFNI CGCG 1 cut(s) 978
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 8 cut(s) 67, 231, 667, 781, 1040, 1128, 1398, 1526
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
BstMWI GCNNNNNNNGC 4 cut(s) 474, 547, 863, 1163
BstNI CCWGG 2 cut(s) 383, 416
BstNSI RCATGY 3 cut(s) 20, 659, 800
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 381, 414
BstSFI CTRYAG 2 cut(s) 44, 297
BstSLI GKGCMC 2 cut(s) 424, 1368
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 3 cut(s) 280, 787, 1202
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 3 cut(s) 112, 468, 1308
BtgI CCRYGG 1 cut(s) 806
BtsCI GGATG 2 cut(s) 52, 600
BtsI GCAGTG 2 cut(s) 652, 1460
BtsIMutI CAGTG 7 cut(s) 469, 652, 720, 1026, 1460, 1483, 1602
BveI ACCTGC 3 cut(s) 304, 970, 1220
Cac8I GCNNGC 4 cut(s) 787, 1117, 1241, 1502
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 2 cut(s) 1306, 1358
Csp6I GTAC 2 cut(s) 285, 625
CspAI ACCGGT 1 cut(s) 911
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 558, 593
CviAII CATG 7 cut(s) 17, 656, 797, 807, 1123, 1303, 1393
CviQI GTAC 2 cut(s) 285, 625
DdeI CTNAG 5 cut(s) 114, 192, 759, 1225, 1235
DpnI GATC 8 cut(s) 66, 230, 666, 780, 1039, 1127, 1397, 1525
DpnII GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 2 cut(s) 107, 806
Eco24I GRGCYC 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 1358
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 2 cut(s) 1229, 1578
Eco81I CCTNAGG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 1080
EcoICRI GAGCTC 1 cut(s) 842
EcoRII CCWGG 2 cut(s) 381, 414
EcoT14I CCWWGG 2 cut(s) 107, 806
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 2 cut(s) 107, 806
FaeI CATG 7 cut(s) 20, 659, 800, 810, 1126, 1306, 1396
FaqI GGGAC 2 cut(s) 311, 1526
FatI CATG 7 cut(s) 16, 655, 796, 806, 1122, 1302, 1392
FauI CCCGC 1 cut(s) 722
FbaI TGATCA 1 cut(s) 1395
Fnu4HI GCNGC 3 cut(s) 269, 801, 1191
FokI GGATG 2 cut(s) 39, 587
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 3 cut(s) 269, 801, 1191
GlaI GCGC 1 cut(s) 476
GluI GCNGC 3 cut(s) 269, 801, 1191
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 3 cut(s) 112, 468, 1308
HapII CCGG 2 cut(s) 912, 1361
HhaI GCGC 1 cut(s) 477
Hin1II CATG 7 cut(s) 20, 659, 800, 810, 1126, 1306, 1396
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 5 cut(s) 255, 376, 905, 1078, 1518
HpaI GTTAAC 1 cut(s) 765
HpaII CCGG 2 cut(s) 912, 1361
HphI GGTGA 3 cut(s) 160, 496, 700
Hpy166II GTNNAC 7 cut(s) 14, 293, 422, 500, 765, 909, 1366
Hpy188I TCNGA 4 cut(s) 195, 639, 1417, 1523
Hpy188III TCNNGA 4 cut(s) 232, 1123, 1270, 1451
Hpy8I GTNNAC 7 cut(s) 14, 293, 422, 500, 765, 909, 1366
Hpy99I CGWCG 1 cut(s) 1486
HpyAV CCTTC 3 cut(s) 614, 1089, 1602
HpyCH4III ACNGT 6 cut(s) 243, 511, 624, 724, 1265, 1478
HpyCH4IV ACGT 1 cut(s) 1218
HpyF10VI GCNNNNNNNGC 4 cut(s) 474, 547, 863, 1163
HpyF3I CTNAG 5 cut(s) 114, 192, 759, 1225, 1235
HpySE526I ACGT 1 cut(s) 1218
Hsp92II CATG 7 cut(s) 20, 659, 800, 810, 1126, 1306, 1396
HspAI GCGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 1395
KspAI GTTAAC 1 cut(s) 765
Kzo9I GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
LmnI GCTCC 8 cut(s) 40, 308, 808, 839, 847, 1154, 1373, 1417
Lsp1109I GCAGC 3 cut(s) 280, 787, 1202
LweI GCATC 6 cut(s) 141, 320, 798, 844, 1421, 1474
MaeII ACGT 1 cut(s) 1218
MaeIII GTNAC 1 cut(s) 688
MalI GATC 8 cut(s) 66, 230, 666, 780, 1039, 1127, 1397, 1525
MboI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
MboII GAAGA 3 cut(s) 1120, 1198, 1201
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 3 cut(s) 424, 844, 1368
MluCI AATT 7 cut(s) 303, 389, 693, 1107, 1148, 1277, 1296
MlyI GAGTC 2 cut(s) 914, 1072
MmeI TCCRAC 1 cut(s) 160
MseI TTAA 3 cut(s) 764, 971, 1347
MspI CCGG 2 cut(s) 912, 1361
MspR9I CCNGG 2 cut(s) 383, 416
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 2 cut(s) 383, 416
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 4 cut(s) 474, 547, 863, 1163
NcoI CCATGG 1 cut(s) 806
NdeII GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
NlaIII CATG 7 cut(s) 20, 659, 800, 810, 1126, 1306, 1396
NlaIV GGNNCC 3 cut(s) 412, 491, 1419
NmuCI GTSAC 1 cut(s) 688
NspI RCATGY 3 cut(s) 20, 659, 800
PaeR7I CTCGAG 1 cut(s) 1080
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 970
PasI CCCWGGG 1 cut(s) 415
PciI ACATGT 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PctI GAATGC 1 cut(s) 1002
PfeI GAWTC 3 cut(s) 255, 376, 1518
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 3 cut(s) 270, 802, 1192
PleI GAGTC 2 cut(s) 913, 1072
PpsI GAGTC 2 cut(s) 913, 1072
PscI ACATGT 1 cut(s) 655
PshAI GACNNNNGTC 1 cut(s) 656
PsiI TTATAA 2 cut(s) 347, 738
Psp124BI GAGCTC 1 cut(s) 844
Psp6I CCWGG 2 cut(s) 381, 414
PspGI CCWGG 2 cut(s) 381, 414
PspN4I GGNNCC 3 cut(s) 412, 491, 1419
PspPI GGNCC 2 cut(s) 1306, 1358
PspXI VCTCGAGB 1 cut(s) 1080
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 1049
RsaI GTAC 2 cut(s) 286, 626
RsaNI GTAC 2 cut(s) 285, 625
SacI GAGCTC 1 cut(s) 844
SaqAI TTAA 3 cut(s) 764, 971, 1347
SatI GCNGC 3 cut(s) 269, 801, 1191
Sau3AI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Sau96I GGNCC 2 cut(s) 1306, 1358
SchI GAGTC 2 cut(s) 914, 1072
ScrFI CCNGG 2 cut(s) 383, 416
SduI GDGCHC 3 cut(s) 424, 844, 1368
SfaNI GCATC 6 cut(s) 141, 320, 798, 844, 1421, 1474
SfcI CTRYAG 2 cut(s) 44, 297
Sfr274I CTCGAG 1 cut(s) 1080
SgrAI CRCCGGYG 1 cut(s) 911
SinI GGWCC 1 cut(s) 1358
SlaI CTCGAG 1 cut(s) 1080
SmlI CTYRAG 1 cut(s) 1080
SmoI CTYRAG 1 cut(s) 1080
Sse9I AATT 7 cut(s) 303, 389, 693, 1107, 1148, 1277, 1296
SsiI CCGC 2 cut(s) 729, 978
SspI AATATT 1 cut(s) 1325
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 381, 414
StyI CCWWGG 2 cut(s) 107, 806
TaaI ACNGT 6 cut(s) 243, 511, 624, 724, 1265, 1478
TaiI ACGT 1 cut(s) 1221
TaqI TCGA 6 cut(s) 358, 772, 932, 1081, 1169, 1468
TasI AATT 7 cut(s) 303, 389, 693, 1107, 1148, 1277, 1296
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 3 cut(s) 255, 376, 1518
Tru1I TTAA 3 cut(s) 764, 971, 1347
Tru9I TTAA 3 cut(s) 764, 971, 1347
TscAI CASTG 7 cut(s) 469, 652, 727, 1033, 1467, 1483, 1602
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 3 cut(s) 268, 800, 1190
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 876, 1111, 1291, 1347
TspRI CASTG 7 cut(s) 469, 652, 727, 1033, 1467, 1483, 1602
VneI GTGCAC 2 cut(s) 420, 1364
VpaK11BI GGWCC 1 cut(s) 1358
XapI RAATTY 4 cut(s) 389, 1107, 1148, 1277
XceI RCATGY 3 cut(s) 20, 659, 800
XhoI CTCGAG 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.