RLG00000005835

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005215
Physical Location & Seq
Reverse (-)
2224764 .. 2228850
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005835

Sequence Viewer

Length: 1725 bp
ATGTTTCTTGGGCTCCAACTTGAGCCAACTTTCAGTGGTTTTTCCAAGTTTTGGGCTTTCCTTTGGAACTTGCTACTTTGCTTCATCAAAAAGAGCAGGAGCAGGAGAGAACAAGAGAGGAACATGGCAGGAGGTGGACATGCACCACCGCCGAAACAGGAGGAGCTACAGCCTCATCCAGTAAAAGATCAGCTACCTAACATTTCCTACTGCATTACCAGTCCTCCTCCTTGGCCTGAGGCTATCCTACTTGGTTTCCAACATTACTTGGTGATGCTTGGCACAGCAGTTCTCATTCCCACCTATCTTGTTCCTCAGATGGGAGGAGGAAATGAGGAGAAAGCAAAAATGATCCAGACTATACTGTTTGTTGCTGGAATCAACACATTGCAGCAGACATTTTTTGGGACTCGTTTACCTGCAGTAATTGGAGCATCTTATACCTATGTCCCAACAACCATTTCTATTATCTTGGCTGGTCGATATAGTGATGTTGTGAATCCCCAGGAGAAATTCGAGAAAATAATGCGTGGAACCCAGGGTGCACTCATTGTTGCCTCTATTCTGCAGATTGTTGTTGGTTTCAGTGGCCTTTGGCGCAATGTGGCGAGGTTCCAAAGTCCACTTTCTACTGTTCCTTTGGTTGCTTTGTCAGGCTTTGGGCTATATGAGCTTGGTTTTCCTGTGTTTGCAAAATGTGTGGAGATTGGGCTACCACAACTCATCCTTCTACTGATATTTTCACAGTACATACCTCATCTGATGCGCAGTGAGACGCATGTCTTTGATCGCTTTGCTGTTTTATTTTCGGTGGCAATTGTGTGGATTTATGCTCATCTGCTCACTGTGGGCGGGGCTTATAAGAACACACCAGAAGAAACTCAGTTAACCTGTCGAACTGATCGTGCTGGCATCATACATGCTGCTCCATGGATAAGAGTTCCTTATCCTTTCCAATGGGGAGCTCCCACTTTTGATGCTGGAGAAGCTTTTGCAATGATGACTGCTTCATTTGTTGCTCTTGTAGAGTCCACCGGTGTCTTTATTGCTGTGTCGAGGTATGCAAGTGCAACTCCACTCCCACCTGCTATTCTAAGTCGCGGTATTGGTTGGCAGGGAGTGGGGATTCTTTTCTCTGGTATATTCGGCACTGGGAATGGATCATCAGTATCTGTTGAAAATGCTGGTTTGTTAGCTTTGACTCGAGTTGGTAGCCGAAGGGTTGTCCAAATTTCAGCCAGCTTCATGATCTTCTTTTCCATACTTGGAAAATTTGGAGCTGTCTTTGCCTCGATACCAGCACCCATTGTTGCAGCAATGTATTGCTTTTTCTTTGCTTATGTTGGCTCAGCAGGTCTTAGCTTGCTTCAGTTTTGCAATCTAAACAGTTTCAGGACCAAATTTATCATAGGCTTCTCAATTTTCATGGGCCTTTCAATACCACAATATTTCAATGAATATACCCTTGTTAAAGGCTATGGTCCGGTGCACACAGGAGCAAGATGGTTCAATGACATGATCAATGTTCCTTTTGCATCTGAGCCATTTGTTGCTGGTTTATTGGCATTTGTCCTGGACACCACACTGCATCGAAAGGACACTGCAACGAGGAAAGACAGGGGCTTGCATTGGTGGGACAGATTCCGATCATACAAGACAGATACAAGAAGTGAGGAGTTCTATTCCTTGCCTTTCAACCTCAACAAGTTTTTCCCTTCAGTGTGA

Protein Analysis

575

Amino Acids

63.6

Weight (kDa)

9.35

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 81 - 484 6e-66 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 861
AarI CACCTGC 1 cut(s) 1093
Acc16I TGCGCA 1 cut(s) 767
Acc36I ACCTGC 3 cut(s) 427, 1093, 1343
AccB7I CCANNNNNTGG 1 cut(s) 51
AccII CGCG 1 cut(s) 1101
AciI CCGC 3 cut(s) 149, 852, 1101
AclWI GGATC 2 cut(s) 346, 1168
AcsI RAATTY 4 cut(s) 512, 1230, 1271, 1400
AcuI CTGAAG 2 cut(s) 1352, 1701
AfaI GTAC 1 cut(s) 749
AfiI CCNNNNNNNGG 2 cut(s) 51, 320
AgeI ACCGGT 1 cut(s) 1034
AgsI TTSAA 5 cut(s) 1178, 1437, 1453, 1510, 1696
AjnI CCWGG 3 cut(s) 504, 537, 1572
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 9 cut(s) 166, 193, 673, 965, 989, 1196, 1242, 1280, 1362
AluI AGCT 9 cut(s) 166, 193, 673, 965, 989, 1196, 1242, 1280, 1362
Alw21I GWGCWC 3 cut(s) 547, 967, 1491
Alw26I GTCTC 1 cut(s) 767
Alw44I GTGCAC 2 cut(s) 543, 1487
AlwI GGATC 2 cut(s) 346, 1168
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 1 cut(s) 1203
AoxI GGCC 3 cut(s) 233, 589, 1429
ApaLI GTGCAC 2 cut(s) 543, 1487
ApeKI GCWGC 3 cut(s) 391, 923, 1313
ApoI RAATTY 4 cut(s) 512, 1230, 1271, 1400
AsiGI ACCGGT 1 cut(s) 1034
AspLEI GCGC 2 cut(s) 600, 768
AspS9I GGNCC 3 cut(s) 1395, 1429, 1481
AsuHPI GGTGA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 1203
AvaII GGWCC 2 cut(s) 1395, 1481
AxyI CCTNAGG 1 cut(s) 237
BaeGI GKGCMC 2 cut(s) 547, 1491
BanII GRGCYC 2 cut(s) 15, 967
Bbv12I GWGCWC 3 cut(s) 547, 967, 1491
BbvI GCAGC 3 cut(s) 403, 910, 1325
BccI CCATC 2 cut(s) 313, 1497
BciT130I CCWGG 3 cut(s) 506, 539, 1574
BclI TGATCA 1 cut(s) 1518
BcoDI GTCTC 1 cut(s) 767
BfmI CTRYAG 3 cut(s) 167, 420, 566
BfuAI ACCTGC 3 cut(s) 427, 1093, 1343
BisI GCNGC 3 cut(s) 392, 924, 1314
BlpI GCTNAGC 1 cut(s) 1348
BlsI GCNGC 3 cut(s) 393, 925, 1315
Bme1390I CCNGG 3 cut(s) 506, 539, 1574
Bme18I GGWCC 2 cut(s) 1395, 1481
BmeT110I CYCGRG 1 cut(s) 1203
BmgT120I GGNCC 3 cut(s) 1395, 1429, 1481
BmiI GGNNCC 3 cut(s) 14, 535, 614
BmrFI CCNGG 3 cut(s) 506, 539, 1574
BmrI ACTGGG 1 cut(s) 1161
BmsI GCATC 7 cut(s) 264, 443, 753, 921, 967, 1544, 1597
BmuI ACTGGG 1 cut(s) 1161
BoxI GACNNNNGTC 1 cut(s) 779
BpmI CTGGAG 1 cut(s) 1002
Bpu1102I GCTNAGC 1 cut(s) 1348
BpuEI CTTGAG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 1645
BsaJI CCNNGG 5 cut(s) 230, 504, 537, 538, 929
BsaWI WCCGGW 2 cut(s) 1034, 1483
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 320
Bse118I RCCGGY 1 cut(s) 1034
Bse1I ACTGG 3 cut(s) 179, 219, 1156
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 4 cut(s) 386, 607, 1002, 1323
Bse8I GATNNNNATC 1 cut(s) 1645
BseBI CCWGG 3 cut(s) 506, 539, 1574
BseDI CCNNGG 5 cut(s) 230, 504, 537, 538, 929
BseGI GGATG 2 cut(s) 175, 723
BseJI GATNNNNATC 1 cut(s) 1645
BseLI CCNNNNNNNGG 2 cut(s) 51, 320
BseMI GCAATG 4 cut(s) 386, 607, 1002, 1323
BseMII CTCAG 5 cut(s) 228, 329, 896, 1362, 1530
BseNI ACTGG 3 cut(s) 179, 219, 1156
BseRI GAGGAG 5 cut(s) 176, 216, 339, 350, 1688
BseSI GKGCMC 2 cut(s) 547, 1491
BseXI GCAGC 3 cut(s) 403, 910, 1325
Bsh1236I CGCG 1 cut(s) 1101
BshFI GGCC 3 cut(s) 235, 591, 1431
BshTI ACCGGT 1 cut(s) 1034
BsiHKAI GWGCWC 3 cut(s) 547, 967, 1491
BsiHKCI CYCGRG 1 cut(s) 1203
BsiSI CCGG 2 cut(s) 1035, 1484
BslFI GGGAC 3 cut(s) 421, 434, 1649
BslI CCNNNNNNNGG 2 cut(s) 51, 320
BsmAI GTCTC 1 cut(s) 767
BsmBI CGTCTC 1 cut(s) 767
BsmFI GGGAC 3 cut(s) 421, 434, 1649
BsnI GGCC 3 cut(s) 235, 591, 1431
BsoBI CYCGRG 1 cut(s) 1203
Bsp1286I GDGCHC 4 cut(s) 15, 547, 967, 1491
Bsp143I GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
Bsp1720I GCTNAGC 1 cut(s) 1348
Bsp19I CCATGG 1 cut(s) 929
BspACI CCGC 3 cut(s) 149, 852, 1101
BspANI GGCC 3 cut(s) 235, 591, 1431
BspCNI CTCAG 5 cut(s) 229, 328, 895, 1361, 1531
BspFNI CGCG 1 cut(s) 1101
BspHI TCATGA 1 cut(s) 1245
BspLI GGNNCC 3 cut(s) 14, 535, 614
BspMAI CTGCAG 2 cut(s) 424, 570
BspMI ACCTGC 3 cut(s) 427, 1093, 1343
BspPI GGATC 2 cut(s) 346, 1168
BsrDI GCAATG 4 cut(s) 386, 607, 1002, 1323
BsrFI RCCGGY 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 179, 219, 1156
BssAI RCCGGY 1 cut(s) 1034
BssECI CCNNGG 5 cut(s) 230, 504, 537, 538, 929
BssMI GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
BssT1I CCWWGG 2 cut(s) 230, 929
Bst2UI CCWGG 3 cut(s) 506, 539, 1574
Bst4CI ACNGT 5 cut(s) 366, 634, 747, 847, 1388
BstC8I GCNNGC 4 cut(s) 910, 1240, 1364, 1625
BstDEI CTNAG 7 cut(s) 237, 315, 882, 1094, 1348, 1358, 1539
BstDSI CCRYGG 1 cut(s) 929
BstF5I GGATG 2 cut(s) 175, 723
BstFNI CGCG 1 cut(s) 1101
BstHHI GCGC 2 cut(s) 600, 768
BstKTI GATC 8 cut(s) 190, 354, 790, 904, 1163, 1251, 1521, 1649
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
BstMWI GCNNNNNNNGC 4 cut(s) 597, 670, 986, 1286
BstNI CCWGG 3 cut(s) 506, 539, 1574
BstNSI RCATGY 3 cut(s) 143, 782, 923
BstPAI GACNNNNGTC 1 cut(s) 779
BstSCI CCNGG 3 cut(s) 504, 537, 1572
BstSFI CTRYAG 3 cut(s) 167, 420, 566
BstSLI GKGCMC 2 cut(s) 547, 1491
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 3 cut(s) 403, 910, 1325
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 3 cut(s) 235, 591, 1431
BtgI CCRYGG 1 cut(s) 929
BtsCI GGATG 2 cut(s) 175, 723
BtsI GCAGTG 3 cut(s) 775, 1583, 1599
BtsIMutI CAGTG 8 cut(s) 40, 592, 775, 843, 1149, 1583, 1599, 1725
BveI ACCTGC 3 cut(s) 427, 1093, 1343
Cac8I GCNNGC 4 cut(s) 910, 1240, 1364, 1625
CaiI CAGNNNCTG 1 cut(s) 1172
CciI TCATGA 1 cut(s) 1245
CfoI GCGC 2 cut(s) 600, 768
Cfr10I RCCGGY 1 cut(s) 1034
Cfr13I GGNCC 3 cut(s) 1395, 1429, 1481
CseI GACGC 1 cut(s) 784
Csp6I GTAC 1 cut(s) 748
CspAI ACCGGT 1 cut(s) 1034
CspCI CAANNNNNGTGG 4 cut(s) 289, 324, 681, 716
CviAII CATG 8 cut(s) 124, 140, 779, 920, 930, 1246, 1426, 1516
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 7 cut(s) 237, 315, 882, 1094, 1348, 1358, 1539
DpnI GATC 8 cut(s) 189, 353, 789, 903, 1162, 1250, 1520, 1648
DpnII GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
Ecl136II GAGCTC 1 cut(s) 965
Eco130I CCWWGG 2 cut(s) 230, 929
Eco24I GRGCYC 2 cut(s) 15, 967
Eco47I GGWCC 2 cut(s) 1395, 1481
Eco53kI GAGCTC 1 cut(s) 965
Eco57I CTGAAG 2 cut(s) 1352, 1701
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 1203
EcoICRI GAGCTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 504, 537, 1572
EcoT14I CCWWGG 2 cut(s) 230, 929
EcoT38I GRGCYC 2 cut(s) 15, 967
ErhI CCWWGG 2 cut(s) 230, 929
Esp3I CGTCTC 1 cut(s) 767
FaeI CATG 8 cut(s) 127, 143, 782, 923, 933, 1249, 1429, 1519
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 3 cut(s) 421, 434, 1649
FatI CATG 8 cut(s) 123, 139, 778, 919, 929, 1245, 1425, 1515
FauI CCCGC 1 cut(s) 845
FbaI TGATCA 1 cut(s) 1518
Fnu4HI GCNGC 3 cut(s) 392, 924, 1314
FokI GGATG 2 cut(s) 162, 710
FriOI GRGCYC 2 cut(s) 15, 967
Fsp4HI GCNGC 3 cut(s) 392, 924, 1314
FspI TGCGCA 1 cut(s) 767
GlaI GCGC 2 cut(s) 599, 767
GluI GCNGC 3 cut(s) 392, 924, 1314
GsuI CTGGAG 1 cut(s) 1002
HaeIII GGCC 3 cut(s) 235, 591, 1431
HapII CCGG 2 cut(s) 1035, 1484
HgaI GACGC 1 cut(s) 784
HhaI GCGC 2 cut(s) 600, 768
Hin1II CATG 8 cut(s) 127, 143, 782, 923, 933, 1249, 1429, 1519
Hin6I GCGC 2 cut(s) 598, 766
HinP1I GCGC 2 cut(s) 598, 766
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HindIII AAGCTT 1 cut(s) 987
HinfI GANTC 7 cut(s) 378, 409, 499, 1028, 1126, 1201, 1641
HpaI GTTAAC 1 cut(s) 888
HpaII CCGG 2 cut(s) 1035, 1484
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 7 cut(s) 137, 416, 545, 623, 888, 1032, 1489
Hpy188I TCNGA 4 cut(s) 318, 762, 1540, 1646
Hpy188III TCNNGA 4 cut(s) 355, 517, 1246, 1393
Hpy8I GTNNAC 7 cut(s) 137, 416, 545, 623, 888, 1032, 1489
HpyAV CCTTC 3 cut(s) 737, 1212, 1725
HpyCH4III ACNGT 5 cut(s) 366, 634, 747, 847, 1388
HpyF10VI GCNNNNNNNGC 4 cut(s) 597, 670, 986, 1286
HpyF3I CTNAG 7 cut(s) 237, 315, 882, 1094, 1348, 1358, 1539
Hsp92II CATG 8 cut(s) 127, 143, 782, 923, 933, 1249, 1429, 1519
HspAI GCGC 2 cut(s) 598, 766
Ksp22I TGATCA 1 cut(s) 1518
KspAI GTTAAC 1 cut(s) 888
Kzo9I GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
LmnI GCTCC 9 cut(s) 18, 99, 163, 431, 931, 962, 970, 1277, 1496
Lsp1109I GCAGC 3 cut(s) 403, 910, 1325
LweI GCATC 7 cut(s) 264, 443, 753, 921, 967, 1544, 1597
MalI GATC 8 cut(s) 189, 353, 789, 903, 1162, 1250, 1520, 1648
MboI GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
MboII GAAGA 2 cut(s) 887, 1243
MfeI CAATTG 1 cut(s) 816
MhlI GDGCHC 4 cut(s) 15, 547, 967, 1491
MluCI AATT 7 cut(s) 426, 512, 816, 1230, 1271, 1400, 1419
MlyI GAGTC 3 cut(s) 403, 1037, 1195
MmeI TCCRAC 2 cut(s) 40, 283
MseI TTAA 2 cut(s) 887, 1470
MspI CCGG 2 cut(s) 1035, 1484
MspR9I CCNGG 3 cut(s) 506, 539, 1574
MunI CAATTG 1 cut(s) 816
MvaI CCWGG 3 cut(s) 506, 539, 1574
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 4 cut(s) 597, 670, 986, 1286
NcoI CCATGG 1 cut(s) 929
NdeII GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
NlaIII CATG 8 cut(s) 127, 143, 782, 923, 933, 1249, 1429, 1519
NlaIV GGNNCC 3 cut(s) 14, 535, 614
NsbI TGCGCA 1 cut(s) 767
NspI RCATGY 3 cut(s) 143, 782, 923
PaeR7I CTCGAG 1 cut(s) 1203
PagI TCATGA 1 cut(s) 1245
PaqCI CACCTGC 1 cut(s) 1093
PasI CCCWGGG 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 901
PfeI GAWTC 4 cut(s) 378, 499, 1126, 1641
PflMI CCANNNNNTGG 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 1572
PinAI ACCGGT 1 cut(s) 1034
PkrI GCNGC 3 cut(s) 393, 925, 1315
PleI GAGTC 3 cut(s) 403, 1036, 1195
PpsI GAGTC 3 cut(s) 403, 1036, 1195
PshAI GACNNNNGTC 1 cut(s) 779
PsiI TTATAA 1 cut(s) 861
Psp124BI GAGCTC 1 cut(s) 967
Psp6I CCWGG 3 cut(s) 504, 537, 1572
PspGI CCWGG 3 cut(s) 504, 537, 1572
PspN4I GGNNCC 3 cut(s) 14, 535, 614
PspPI GGNCC 3 cut(s) 1395, 1429, 1481
PspXI VCTCGAGB 1 cut(s) 1203
PstI CTGCAG 2 cut(s) 424, 570
PstNI CAGNNNCTG 1 cut(s) 1172
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
SacI GAGCTC 1 cut(s) 967
SaqAI TTAA 2 cut(s) 887, 1470
SatI GCNGC 3 cut(s) 392, 924, 1314
Sau3AI GATC 8 cut(s) 187, 351, 787, 901, 1160, 1248, 1518, 1646
Sau96I GGNCC 3 cut(s) 1395, 1429, 1481
SchI GAGTC 3 cut(s) 403, 1037, 1195
ScrFI CCNGG 3 cut(s) 506, 539, 1574
SduI GDGCHC 4 cut(s) 15, 547, 967, 1491
SfaNI GCATC 7 cut(s) 264, 443, 753, 921, 967, 1544, 1597
SfcI CTRYAG 3 cut(s) 167, 420, 566
Sfr274I CTCGAG 1 cut(s) 1203
SgrAI CRCCGGYG 1 cut(s) 1034
SinI GGWCC 2 cut(s) 1395, 1481
SlaI CTCGAG 1 cut(s) 1203
SmlI CTYRAG 2 cut(s) 20, 1203
SmoI CTYRAG 2 cut(s) 20, 1203
Sse9I AATT 7 cut(s) 426, 512, 816, 1230, 1271, 1400, 1419
SsiI CCGC 3 cut(s) 149, 852, 1101
SspI AATATT 1 cut(s) 1448
SstI GAGCTC 1 cut(s) 967
StyD4I CCNGG 3 cut(s) 504, 537, 1572
StyI CCWWGG 2 cut(s) 230, 929
TaaI ACNGT 5 cut(s) 366, 634, 747, 847, 1388
TaqI TCGA 7 cut(s) 481, 516, 895, 1055, 1204, 1292, 1591
TasI AATT 7 cut(s) 426, 512, 816, 1230, 1271, 1400, 1419
TatI WGTACW 1 cut(s) 747
TfiI GAWTC 4 cut(s) 378, 499, 1126, 1641
Tru1I TTAA 2 cut(s) 887, 1470
Tru9I TTAA 2 cut(s) 887, 1470
TscAI CASTG 8 cut(s) 40, 592, 775, 850, 1156, 1590, 1606, 1725
TseI GCWGC 3 cut(s) 391, 923, 1313
TspDTI ATGAA 5 cut(s) 73, 999, 1234, 1414, 1470
TspRI CASTG 8 cut(s) 40, 592, 775, 850, 1156, 1590, 1606, 1725
Van91I CCANNNNNTGG 1 cut(s) 51
VneI GTGCAC 2 cut(s) 543, 1487
VpaK11BI GGWCC 2 cut(s) 1395, 1481
XapI RAATTY 4 cut(s) 512, 1230, 1271, 1400
XceI RCATGY 3 cut(s) 143, 782, 923
XhoI CTCGAG 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.