FvH4_4g34940

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005215
Physical Location & Seq
Forward (+)
32443348 .. 32448454
5107 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34940.t2

Sequence Viewer

Length: 1602 bp
ATGGCTGGAGGTGGACATGCACCACCGCCGAAACAGGAGGAACTACAGCCTCATCCAGTAAAAGATCAGCTACCAAACATTTCCTACTGCATTACCAGTCCTCCTCCTTGGCCTGAGGCTATTCTACTTGGTTTCCAACATTACTTGGTGATGCTCGGCACAACAGTTCTCATTCCCACCTATCTCGTTCCTCAGATGGGAGGAGGAAACGAGGAGAAAGCAAAAATGATCCAGACTATACTGTTTGTTGCTGGAATCAACACATTGCAGCAGACATTTTTTGGGACTCGTTTACCTGCAGTTATTGGAGCATCTTATACCTATGTCCCAACAACCATTTCGATTATTTTGGCTGGTCGATATAGTGATGTTGTGAACCCCCAGGAGAAATTCGAGAAGATAATGCGTGGAACCCAGGGTGCACTTATTGTTGCCTCTATACTGCAGATTGTTGTTGGCTTCAGTGGCCTCTGGCGTAATGTGGCAAGGTTCCTAAGTCCACTTTCTGCTGTTCCTTTGGTTGCTTTGTCAGGCTTTGGGCTATATGAGCTTGGATTTCCTGTGTTTTCAAAATGTGTGGAGATTGGCTTACCACAACTCATCCTTCTACTGATATTTTCACAGTACATACCTCATCTGATGCACAGTGAGACGCATGTCTTCGATCGCTTTGCTGTTTTATTTTCGGTGGCAATTGTGTGGATTTATGCTCATCTGCTCACTGTGGGTGGGGCTTATAAGCACACACCAGAAGCAACTCAGTTAAGCTGTCGAACTGATCGTGCTGGCATCATACATGCTGCTCCATGGATAAGAGTTCCATATCCTTTTCAATGGGGAGCTCCGACTTTTGATGCTGGAGAAGCTTTTGCAATGATGACTGCTTCATTTGTTGCTCTTGTTGAGTCCACCGGTGTCTTTATTGCTGTGTCGAGGTATGCAAGTGCAACTCCGCTCCCACCTGCTATATTAAGTCGCGGTATTGGTTGGCAGGGTGTGGGCATTCTTTTCTCTGGTATATTTGGCACTGGGAATGGATCATCAGTATCTGTTGAAAATGCTGGTTTGTTAGCTTTGACACGGGTTGGCAGCCGAAGAGTTGTCCAAATTTCAGCCAGCTTCATGATCTTCTTTTCCATACTTGGAAAATTTGGAGCTGTCTTTGCCTCGATACCAGCACCCATTGTTGCAGCAATGTATTGCTTCTTCTTCGCTTATGTTGGTTCAGCAGGTCTTAGCTTGCTTCAGTTTTGCAATCTAAACAGTTTCAGGACAAAGTTTATCATAGGCTTCTCAATTTTCATGGGCCTTTCAGTACCACAGTATTTCAATGAATATACACTTGTTAAAGGCTATGGTCCGGTGCACACAGGAGCAAGATGGTTCAATGACATGATCAATGTACCTTTTTCTTCGGAGCCATTTGTTGCTGGTTTATTGGCATTTGTCTTGGACACAACACTGCATCGAAAGGACAATGCGACCAGGAAAGACAGAGGCATGCCTTGGTGGGATAGATTCCGATCATACAAGACAGATACAAGAAGTGAGGAATTCTATTCCTTGCCTTTCAACCTCAACAAGTTCTTCCCTTCAGTGTGA

Protein Analysis

534

Amino Acids

58.52

Weight (kDa)

9.14

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 443 5.6e-66 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 3 cut(s) 304, 970, 1220
AccBSI CCGCTC 1 cut(s) 955
AccII CGCG 1 cut(s) 978
AciI CCGC 3 cut(s) 26, 953, 978
AclWI GGATC 2 cut(s) 223, 1045
AcsI RAATTY 4 cut(s) 389, 1107, 1148, 1553
AcuI CTGAAG 3 cut(s) 445, 1229, 1578
AfaI GTAC 3 cut(s) 626, 1317, 1404
AfiI CCNNNNNNNGG 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 911
AgsI TTSAA 6 cut(s) 570, 833, 1055, 1330, 1387, 1573
AjnI CCWGG 3 cut(s) 381, 414, 1484
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 9 cut(s) 70, 550, 768, 842, 866, 1073, 1119, 1157, 1239
AluI AGCT 9 cut(s) 70, 550, 768, 842, 866, 1073, 1119, 1157, 1239
Alw21I GWGCWC 3 cut(s) 424, 844, 1368
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 2 cut(s) 420, 1364
AlwI GGATC 2 cut(s) 223, 1045
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 3 cut(s) 110, 466, 1306
ApaLI GTGCAC 2 cut(s) 420, 1364
ApeKI GCWGC 4 cut(s) 268, 800, 1089, 1190
ApoI RAATTY 4 cut(s) 389, 1107, 1148, 1553
AsiGI ACCGGT 1 cut(s) 911
AspS9I GGNCC 2 cut(s) 1306, 1358
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 1358
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 424, 1368
BanII GRGCYC 1 cut(s) 844
BarI GAAGNNNNNNTAC 2 cut(s) 1190, 1222
BbsI GAAGAC 1 cut(s) 652
Bbv12I GWGCWC 3 cut(s) 424, 844, 1368
BbvI GCAGC 4 cut(s) 280, 787, 1101, 1202
BccI CCATC 2 cut(s) 190, 1374
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 3 cut(s) 383, 416, 1486
BclI TGATCA 1 cut(s) 1395
BcoDI GTCTC 1 cut(s) 644
BfmI CTRYAG 3 cut(s) 44, 297, 443
BfuAI ACCTGC 3 cut(s) 304, 970, 1220
BisI GCNGC 4 cut(s) 269, 801, 1090, 1191
BlsI GCNGC 4 cut(s) 270, 802, 1091, 1192
Bme1390I CCNGG 3 cut(s) 383, 416, 1486
Bme18I GGWCC 1 cut(s) 1358
BmgT120I GGNCC 2 cut(s) 1306, 1358
BmiI GGNNCC 3 cut(s) 412, 491, 1419
BmrFI CCNGG 3 cut(s) 383, 416, 1486
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 6 cut(s) 141, 320, 630, 798, 844, 1474
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 652
BpmI CTGGAG 2 cut(s) 27, 879
BsaBI GATNNNNATC 1 cut(s) 1522
BsaJI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 1505
BsaWI WCCGGW 2 cut(s) 911, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 3 cut(s) 56, 96, 1033
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 3 cut(s) 263, 879, 1200
Bse8I GATNNNNATC 1 cut(s) 1522
BseBI CCWGG 3 cut(s) 383, 416, 1486
BseDI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 1505
BseGI GGATG 2 cut(s) 52, 600
BseJI GATNNNNATC 1 cut(s) 1522
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 3 cut(s) 263, 879, 1200
BseMII CTCAG 3 cut(s) 105, 206, 773
BseNI ACTGG 3 cut(s) 56, 96, 1033
BseRI GAGGAG 3 cut(s) 93, 216, 227
BseSI GKGCMC 2 cut(s) 424, 1368
BseXI GCAGC 4 cut(s) 280, 787, 1101, 1202
Bsh1236I CGCG 1 cut(s) 978
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 3 cut(s) 112, 468, 1308
BshTI ACCGGT 1 cut(s) 911
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 3 cut(s) 424, 844, 1368
BsiSI CCGG 2 cut(s) 912, 1361
BslFI GGGAC 2 cut(s) 298, 311
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 644
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 2 cut(s) 298, 311
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 3 cut(s) 112, 468, 1308
Bsp1286I GDGCHC 3 cut(s) 424, 844, 1368
Bsp143I GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 3 cut(s) 26, 953, 978
BspANI GGCC 3 cut(s) 112, 468, 1308
BspCNI CTCAG 3 cut(s) 106, 205, 772
BspFNI CGCG 1 cut(s) 978
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 3 cut(s) 412, 491, 1419
BspMAI CTGCAG 2 cut(s) 301, 447
BspMI ACCTGC 3 cut(s) 304, 970, 1220
BspPI GGATC 2 cut(s) 223, 1045
BsrBI CCGCTC 1 cut(s) 955
BsrDI GCAATG 3 cut(s) 263, 879, 1200
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 3 cut(s) 56, 96, 1033
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 6 cut(s) 107, 381, 414, 415, 806, 1505
BssMI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
BssT1I CCWWGG 3 cut(s) 107, 806, 1505
Bst2UI CCWGG 3 cut(s) 383, 416, 1486
Bst4CI ACNGT 7 cut(s) 166, 243, 624, 647, 724, 1265, 1323
Bst6I CTCTTC 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 787, 1117, 1241, 1502
BstDEI CTNAG 5 cut(s) 114, 192, 494, 759, 1235
BstDSI CCRYGG 1 cut(s) 806
BstF5I GGATG 2 cut(s) 52, 600
BstFNI CGCG 1 cut(s) 978
BstKTI GATC 8 cut(s) 67, 231, 667, 781, 1040, 1128, 1398, 1526
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 4 cut(s) 465, 547, 863, 1163
BstNI CCWGG 3 cut(s) 383, 416, 1486
BstNSI RCATGY 4 cut(s) 20, 659, 800, 1504
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 381, 414, 1484
BstSFI CTRYAG 3 cut(s) 44, 297, 443
BstSLI GKGCMC 2 cut(s) 424, 1368
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 4 cut(s) 280, 787, 1101, 1202
BstV2I GAAGAC 1 cut(s) 652
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 3 cut(s) 112, 468, 1308
BtgI CCRYGG 1 cut(s) 806
BtsCI GGATG 2 cut(s) 52, 600
BtsI GCAGTG 1 cut(s) 1460
BtsIMutI CAGTG 6 cut(s) 469, 652, 720, 1026, 1460, 1602
BveI ACCTGC 3 cut(s) 304, 970, 1220
Cac8I GCNNGC 4 cut(s) 787, 1117, 1241, 1502
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 1122
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 2 cut(s) 1306, 1358
CseI GACGC 1 cut(s) 661
Csp6I GTAC 3 cut(s) 625, 1316, 1403
CspAI ACCGGT 1 cut(s) 911
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 8 cut(s) 17, 656, 797, 807, 1123, 1303, 1393, 1501
CviQI GTAC 3 cut(s) 625, 1316, 1403
DdeI CTNAG 5 cut(s) 114, 192, 494, 759, 1235
DpnI GATC 8 cut(s) 66, 230, 666, 780, 1039, 1127, 1397, 1525
DpnII GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Eam1104I CTCTTC 1 cut(s) 1090
EarI CTCTTC 1 cut(s) 1090
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 3 cut(s) 107, 806, 1505
Eco24I GRGCYC 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 1358
Eco53kI GAGCTC 1 cut(s) 842
Eco57I CTGAAG 3 cut(s) 445, 1229, 1578
Eco81I CCTNAGG 1 cut(s) 114
EcoICRI GAGCTC 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 1553
EcoRII CCWGG 3 cut(s) 381, 414, 1484
EcoT14I CCWWGG 3 cut(s) 107, 806, 1505
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 3 cut(s) 107, 806, 1505
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 8 cut(s) 20, 659, 800, 810, 1126, 1306, 1396, 1504
FaqI GGGAC 2 cut(s) 298, 311
FatI CATG 8 cut(s) 16, 655, 796, 806, 1122, 1302, 1392, 1500
FbaI TGATCA 1 cut(s) 1395
Fnu4HI GCNGC 4 cut(s) 269, 801, 1090, 1191
FokI GGATG 2 cut(s) 39, 587
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 4 cut(s) 269, 801, 1090, 1191
GluI GCNGC 4 cut(s) 269, 801, 1090, 1191
GsuI CTGGAG 2 cut(s) 27, 879
HaeIII GGCC 3 cut(s) 112, 468, 1308
HapII CCGG 2 cut(s) 912, 1361
HgaI GACGC 1 cut(s) 661
Hin1II CATG 8 cut(s) 20, 659, 800, 810, 1126, 1306, 1396, 1504
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 4 cut(s) 255, 286, 905, 1518
HpaII CCGG 2 cut(s) 912, 1361
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 7 cut(s) 14, 293, 376, 422, 500, 909, 1366
Hpy188I TCNGA 5 cut(s) 195, 639, 846, 1417, 1523
Hpy188III TCNNGA 4 cut(s) 232, 394, 1123, 1270
Hpy8I GTNNAC 7 cut(s) 14, 293, 376, 422, 500, 909, 1366
HpyAV CCTTC 2 cut(s) 614, 1602
HpyCH4III ACNGT 7 cut(s) 166, 243, 624, 647, 724, 1265, 1323
HpyF10VI GCNNNNNNNGC 4 cut(s) 465, 547, 863, 1163
HpyF3I CTNAG 5 cut(s) 114, 192, 494, 759, 1235
Hsp92II CATG 8 cut(s) 20, 659, 800, 810, 1126, 1306, 1396, 1504
Ksp22I TGATCA 1 cut(s) 1395
Kzo9I GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
LmnI GCTCC 8 cut(s) 308, 808, 839, 847, 960, 1154, 1373, 1417
Lsp1109I GCAGC 4 cut(s) 280, 787, 1101, 1202
LweI GCATC 6 cut(s) 141, 320, 630, 798, 844, 1474
MalI GATC 8 cut(s) 66, 230, 666, 780, 1039, 1127, 1397, 1525
MbiI CCGCTC 1 cut(s) 955
MboI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
MboII GAAGA 8 cut(s) 409, 652, 1107, 1120, 1198, 1201, 1404, 1579
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 3 cut(s) 424, 844, 1368
MluCI AATT 6 cut(s) 389, 693, 1107, 1148, 1296, 1553
MlyI GAGTC 2 cut(s) 280, 914
MmeI TCCRAC 2 cut(s) 160, 869
MseI TTAA 3 cut(s) 764, 971, 1347
MspI CCGG 2 cut(s) 912, 1361
MspR9I CCNGG 3 cut(s) 383, 416, 1486
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 3 cut(s) 383, 416, 1486
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 4 cut(s) 465, 547, 863, 1163
NcoI CCATGG 1 cut(s) 806
NdeII GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
NlaIII CATG 8 cut(s) 20, 659, 800, 810, 1126, 1306, 1396, 1504
NlaIV GGNNCC 3 cut(s) 412, 491, 1419
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 4 cut(s) 20, 659, 800, 1504
PaeI GCATGC 1 cut(s) 1504
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 970
PasI CCCWGGG 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PctI GAATGC 1 cut(s) 1002
PfeI GAWTC 2 cut(s) 255, 1518
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 4 cut(s) 270, 802, 1091, 1192
Ple19I CGATCG 1 cut(s) 667
PleI GAGTC 2 cut(s) 280, 913
PpsI GAGTC 2 cut(s) 280, 913
PshAI GACNNNNGTC 1 cut(s) 656
PsiI TTATAA 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 844
Psp6I CCWGG 3 cut(s) 381, 414, 1484
PspGI CCWGG 3 cut(s) 381, 414, 1484
PspN4I GGNNCC 3 cut(s) 412, 491, 1419
PspPI GGNCC 2 cut(s) 1306, 1358
PstI CTGCAG 2 cut(s) 301, 447
PstNI CAGNNNCTG 1 cut(s) 1049
PvuI CGATCG 1 cut(s) 667
RsaI GTAC 3 cut(s) 626, 1317, 1404
RsaNI GTAC 3 cut(s) 625, 1316, 1403
SacI GAGCTC 1 cut(s) 844
SaqAI TTAA 3 cut(s) 764, 971, 1347
SatI GCNGC 4 cut(s) 269, 801, 1090, 1191
Sau3AI GATC 8 cut(s) 64, 228, 664, 778, 1037, 1125, 1395, 1523
Sau96I GGNCC 2 cut(s) 1306, 1358
SchI GAGTC 2 cut(s) 280, 914
ScrFI CCNGG 3 cut(s) 383, 416, 1486
SduI GDGCHC 3 cut(s) 424, 844, 1368
SfaNI GCATC 6 cut(s) 141, 320, 630, 798, 844, 1474
SfcI CTRYAG 3 cut(s) 44, 297, 443
SgrAI CRCCGGYG 1 cut(s) 911
SinI GGWCC 1 cut(s) 1358
SphI GCATGC 1 cut(s) 1504
Sse9I AATT 6 cut(s) 389, 693, 1107, 1148, 1296, 1553
SsiI CCGC 3 cut(s) 26, 953, 978
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 3 cut(s) 381, 414, 1484
StyI CCWWGG 3 cut(s) 107, 806, 1505
TaaI ACNGT 7 cut(s) 166, 243, 624, 647, 724, 1265, 1323
TaqI TCGA 8 cut(s) 341, 358, 393, 663, 772, 932, 1169, 1468
TasI AATT 6 cut(s) 389, 693, 1107, 1148, 1296, 1553
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 2 cut(s) 255, 1518
Tru1I TTAA 3 cut(s) 764, 971, 1347
Tru9I TTAA 3 cut(s) 764, 971, 1347
TscAI CASTG 6 cut(s) 469, 652, 727, 1033, 1467, 1602
TseI GCWGC 4 cut(s) 268, 800, 1089, 1190
TspDTI ATGAA 4 cut(s) 876, 1111, 1291, 1347
TspRI CASTG 6 cut(s) 469, 652, 727, 1033, 1467, 1602
VneI GTGCAC 2 cut(s) 420, 1364
VpaK11BI GGWCC 1 cut(s) 1358
XapI RAATTY 4 cut(s) 389, 1107, 1148, 1553
XceI RCATGY 4 cut(s) 20, 659, 800, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.