AT1G60030

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005215
Physical Location & Seq
Reverse (-)
22113671 .. 22117018
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60030.1

Sequence Viewer

Length: 1617 bp
ATGGCCGGTGGTGGTGGAGGAGGAGGAGGGGTAGCGCCGCCGCTAAAACATGATGGGCTTGAGCCACATCCGGTGAAAGATCAACTTTCTTCTATCTCTTATTGCATCACAAGTCCTCCTCCTTGGCCGGAGGCAATACTTTTGGGGTTTCAGCATTACTTAGTGATGCTTGGAACAACTGTTCTCATCCCAACATATTTAGTTCCACAGATGGGTGGTGGAAATGAAGAGAAGGCAAAGATGGTTCAGACATTACTTTTTGTTTCTGGACTTAATACTTTATTGCAAAGTTTCTTTGGGACTAGACTTCCAGCGGTCATTGGAGGCTCTTACACCTATGTACCAACCACGCTTTCCATCATCTTGGCTGGTCGGTACAGCGACATTTTGGATCCTCAGGAGAAATTTAAGAGGATCATGCGAGGAATCCAGGGTGCTCTTATCGTTGCCTCGATTCTTCAGATCGTTGTTGGCTTCAGCGGGCTTTGGCGCAATGTAGTTAGGCTCCTGAGTCCTCTCTCTGCAGTTCCTCTAGTAGCACTAGCTGGTTTTGGACTTTATGAGCACGGTTTCCCTCTGCTAGCCAAATGCATCGAGATTGGACTGCCTGAGATCATCCTTCTACTCTTATTCTCACAGTACATTCCTCATCTCATTCGAGGAGAAAGACAAGTCTTCCATCGATTTGCTGTGATTTTCTCTGTGGTTATTGTCTGGATTTACGCGCATCTACTTACCGTTGGTGGAGCCTATAAGAACACAGGAGTCAACACTCAGACAAGTTGTAGAACAGATCGTTCTGGCCTTATTAGCGGCTCTCCATGGATAAGAGTTCCTTACCCTTTTCAATGGGGACCTCCAACATTTCATGCCGGTGAAGCTTTCGCTATGATGGCTGTTTCATTTGTTTCCCTAATTGAGTCCACAGGGACTTACATTGTTGTGTCGAGGTTCGCAAGCGCAACTCCGCCCCCGCCTTCCGTTCTCAGCCGTGGGGTTGGTTGGCAGGGTGTAGGAGTTCTTCTCTGTGGACTATTTGGAGCAGGAAATGGAGCATCTGTGTCAGTAGAAAATGCCGGTTTGTTAGCGCTAACACGCGTTGGTAGTAGAAGGGTAGTTCAAATATCAGCCGGTTTTATGATTTTCTTTTCCATTCTTGGTAAATTTGGAGCTATCTTTGCTTCGATTCCAGCTCCAGTAGTCGCAGCATTACATTGCTTGTTTTTTGCATACGTTGGTGCTGGTGGTCTAAGCTTACTTCAGTTCTGTAATCTAAACAGCTTCAGAACAAAATTCATCCTCGGATTCTCGGTCTTCATGGGCTTATCGATACCACAGTACTTCAACCAGTATACAGCTGTTAACAAGTATGGACCAGTTCACACACACGCAAGATGGTTCAATGATATGATCAATGTTCCATTCTCTTCTAAAGCCTTTGTGGCTGGGATTTTGGCATTCTTTCTTGATGTAACAATGTCATCTAAAGACAGTGCGACGAGGAAAGACCGAGGGATGTTTTGGTGGGATCGATTCATGTCGTTCAAATCAGATACTAGAAGTGAAGAGTTTTACTCTTTGCCTTTCAATCTCAACAAGTATTTTCCCTCTGTGTGA

Protein Analysis

538

Amino Acids

58.51

Weight (kDa)

9.51

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 45 - 448 9e-68 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1352
AccII CGCG 2 cut(s) 725, 1098
AciI CCGC 7 cut(s) 38, 41, 314, 480, 813, 968, 974
AclWI GGATC 4 cut(s) 386, 399, 422, 1536
AcoI YGGCCR 2 cut(s) 3, 125
AcsI RAATTY 3 cut(s) 404, 1163, 1292
AcuI CTGAAG 4 cut(s) 443, 460, 1244, 1267
AfaI GTAC 4 cut(s) 342, 377, 641, 1340
AfeI AGCGCT 1 cut(s) 1089
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 1096
AgsI TTSAA 6 cut(s) 848, 1121, 1345, 1402, 1546, 1588
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
AluBI AGCT 7 cut(s) 545, 881, 1172, 1193, 1254, 1281, 1358
AluI AGCT 7 cut(s) 545, 881, 1172, 1193, 1254, 1281, 1358
Alw21I GWGCWC 2 cut(s) 439, 567
AlwI GGATC 4 cut(s) 386, 399, 422, 1536
Aor51HI AGCGCT 1 cut(s) 1089
AoxI GGCC 3 cut(s) 3, 125, 802
ApeKI GCWGC 1 cut(s) 1205
ApoI RAATTY 3 cut(s) 404, 1163, 1292
AspLEI GCGC 5 cut(s) 37, 492, 727, 962, 1090
AspS9I GGNCC 2 cut(s) 854, 1373
AsuHPI GGTGA 2 cut(s) 85, 887
AsuNHI GCTAGC 1 cut(s) 580
AvaII GGWCC 2 cut(s) 854, 1373
AxyI CCTNAGG 1 cut(s) 396
BaeI ACNNNNGTAYC 4 cut(s) 324, 357, 1322, 1355
BamHI GGATCC 1 cut(s) 391
BarI GAAGNNNNNNTAC 2 cut(s) 1005, 1037
BbsI GAAGAC 2 cut(s) 667, 1306
Bbv12I GWGCWC 2 cut(s) 439, 567
BbvI GCAGC 1 cut(s) 1217
BccI CCATC 7 cut(s) 47, 205, 235, 365, 687, 886, 1389
BceAI ACGGC 1 cut(s) 975
BciT130I CCWGG 1 cut(s) 431
BclI TGATCA 1 cut(s) 1410
BfaI CTAG 5 cut(s) 303, 533, 542, 581, 1557
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 2 cut(s) 38, 1091
BglI GCCNNNNNGGC 1 cut(s) 1442
BisI GCNGC 4 cut(s) 38, 41, 814, 1206
BlsI GCNGC 4 cut(s) 39, 42, 815, 1207
BmcAI AGTACT 1 cut(s) 1340
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 2 cut(s) 854, 1373
BmgT120I GGNCC 2 cut(s) 854, 1373
BmiI GGNNCC 4 cut(s) 393, 506, 748, 855
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 5 cut(s) 114, 156, 600, 736, 1064
BmtI GCTAGC 1 cut(s) 584
BpiI GAAGAC 2 cut(s) 667, 1306
BplI GAGNNNNNCTC 4 cut(s) 1008, 1040, 1559, 1591
BpmI CTGGAG 1 cut(s) 1179
BpuEI CTTGAG 1 cut(s) 80
Bsa29I ATCGAT 3 cut(s) 682, 1328, 1531
BsaJI CCNNGG 6 cut(s) 122, 430, 821, 991, 1300, 1510
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 6 cut(s) 15, 45, 100, 130, 1044, 1074
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 4 cut(s) 5, 872, 1076, 1130
Bse1I ACTGG 3 cut(s) 1196, 1348, 1376
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 2 cut(s) 499, 1213
BseBI CCWGG 1 cut(s) 431
BseCI ATCGAT 3 cut(s) 682, 1328, 1531
BseDI CCNNGG 6 cut(s) 122, 430, 821, 991, 1300, 1510
BseGI GGATG 5 cut(s) 67, 186, 615, 1296, 1521
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 2 cut(s) 499, 1213
BseMII CTCAG 5 cut(s) 410, 500, 600, 788, 1000
BseNI ACTGG 3 cut(s) 1196, 1348, 1376
BseRI GAGGAG 5 cut(s) 33, 36, 39, 108, 675
BseXI GCAGC 1 cut(s) 1217
BseYI CCCAGC 1 cut(s) 1445
Bsh1236I CGCG 2 cut(s) 725, 1098
BshFI GGCC 3 cut(s) 5, 127, 804
BshVI ATCGAT 3 cut(s) 682, 1328, 1531
BsiHKAI GWGCWC 2 cut(s) 439, 567
BsiSI CCGG 6 cut(s) 6, 71, 128, 873, 1077, 1131
BslFI GGGAC 3 cut(s) 313, 867, 943
BslI CCNNNNNNNGG 1 cut(s) 212
BsmFI GGGAC 3 cut(s) 313, 867, 943
BsmI GAATGC 1 cut(s) 1457
BsnI GGCC 3 cut(s) 5, 127, 804
Bsp1286I GDGCHC 2 cut(s) 439, 567
Bsp143I GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
Bsp19I CCATGG 1 cut(s) 821
BspACI CCGC 7 cut(s) 38, 41, 314, 480, 813, 968, 974
BspANI GGCC 3 cut(s) 5, 127, 804
BspCNI CTCAG 5 cut(s) 409, 501, 601, 787, 999
BspDI ATCGAT 3 cut(s) 682, 1328, 1531
BspFNI CGCG 2 cut(s) 725, 1098
BspLI GGNNCC 4 cut(s) 393, 506, 748, 855
BspMAI CTGCAG 1 cut(s) 526
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 4 cut(s) 386, 399, 422, 1536
BsrDI GCAATG 2 cut(s) 499, 1213
BsrFI RCCGGY 4 cut(s) 5, 872, 1076, 1130
BsrI ACTGG 3 cut(s) 1196, 1348, 1376
BssAI RCCGGY 4 cut(s) 5, 872, 1076, 1130
BssECI CCNNGG 6 cut(s) 122, 430, 821, 991, 1300, 1510
BssMI GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
BssNAI GTATAC 1 cut(s) 1353
BssT1I CCWWGG 2 cut(s) 122, 821
Bst1107I GTATAC 1 cut(s) 1353
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 6 cut(s) 181, 569, 639, 739, 1338, 1493
Bst6I CTCTTC 3 cut(s) 222, 1432, 1560
BstC8I GCNNGC 3 cut(s) 482, 582, 958
BstDEI CTNAG 7 cut(s) 160, 396, 509, 609, 774, 986, 1250
BstDSI CCRYGG 2 cut(s) 821, 991
BstF5I GGATG 5 cut(s) 67, 186, 615, 1296, 1521
BstFNI CGCG 2 cut(s) 725, 1098
BstH2I RGCGCY 2 cut(s) 38, 1091
BstHHI GCGC 5 cut(s) 37, 492, 727, 962, 1090
BstKTI GATC 8 cut(s) 82, 394, 417, 465, 615, 796, 1413, 1531
BstMBI GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
BstMWI GCNNNNNNNGC 5 cut(s) 810, 878, 893, 1178, 1442
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 2 cut(s) 725, 1098
BstV1I GCAGC 1 cut(s) 1217
BstV2I GAAGAC 2 cut(s) 667, 1306
BstX2I RGATCY 1 cut(s) 391
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 1 cut(s) 391
BstZ17I GTATAC 1 cut(s) 1353
Bsu15I ATCGAT 3 cut(s) 682, 1328, 1531
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 3 cut(s) 5, 127, 804
BsuTUI ATCGAT 3 cut(s) 682, 1328, 1531
BtgI CCRYGG 2 cut(s) 821, 991
BtsCI GGATG 5 cut(s) 67, 186, 615, 1296, 1521
BtsIMutI CAGTG 1 cut(s) 1498
Cac8I GCNNGC 3 cut(s) 482, 582, 958
CfoI GCGC 5 cut(s) 37, 492, 727, 962, 1090
Cfr10I RCCGGY 4 cut(s) 5, 872, 1076, 1130
Cfr13I GGNCC 2 cut(s) 854, 1373
ClaI ATCGAT 3 cut(s) 682, 1328, 1531
Csp6I GTAC 4 cut(s) 341, 376, 640, 1339
CviAII CATG 6 cut(s) 50, 418, 822, 869, 1318, 1537
CviQI GTAC 4 cut(s) 341, 376, 640, 1339
DdeI CTNAG 7 cut(s) 160, 396, 509, 609, 774, 986, 1250
DpnI GATC 8 cut(s) 81, 393, 416, 464, 614, 795, 1412, 1530
DpnII GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
EaeI YGGCCR 2 cut(s) 3, 125
Eam1104I CTCTTC 3 cut(s) 222, 1432, 1560
EarI CTCTTC 3 cut(s) 222, 1432, 1560
EciI GGCGGA 1 cut(s) 957
Eco130I CCWWGG 2 cut(s) 122, 821
Eco47I GGWCC 2 cut(s) 854, 1373
Eco47III AGCGCT 1 cut(s) 1089
Eco57I CTGAAG 4 cut(s) 443, 460, 1244, 1267
Eco81I CCTNAGG 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 122, 821
EcoT22I ATGCAT 1 cut(s) 593
ErhI CCWWGG 2 cut(s) 122, 821
FaeI CATG 6 cut(s) 53, 421, 825, 872, 1321, 1540
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 820, 852
FaqI GGGAC 3 cut(s) 313, 867, 943
FatI CATG 6 cut(s) 49, 417, 821, 868, 1317, 1536
FauI CCCGC 2 cut(s) 473, 981
FbaI TGATCA 1 cut(s) 1410
FblI GTMKAC 1 cut(s) 1352
Fnu4HI GCNGC 4 cut(s) 38, 41, 814, 1206
FokI GGATG 5 cut(s) 54, 173, 602, 1283, 1528
Fsp4HI GCNGC 4 cut(s) 38, 41, 814, 1206
FspBI CTAG 5 cut(s) 303, 533, 542, 581, 1557
GlaI GCGC 5 cut(s) 36, 491, 726, 961, 1089
GluI GCNGC 4 cut(s) 38, 41, 814, 1206
GsaI CCCAGC 1 cut(s) 1449
GsuI CTGGAG 1 cut(s) 1179
HaeII RGCGCY 2 cut(s) 38, 1091
HaeIII GGCC 3 cut(s) 5, 127, 804
HapII CCGG 6 cut(s) 6, 71, 128, 873, 1077, 1131
HhaI GCGC 5 cut(s) 37, 492, 727, 962, 1090
Hin1II CATG 6 cut(s) 53, 421, 825, 872, 1321, 1540
Hin6I GCGC 5 cut(s) 35, 490, 725, 960, 1088
HinP1I GCGC 5 cut(s) 35, 490, 725, 960, 1088
HincII GTYRAC 2 cut(s) 769, 1363
HindII GTYRAC 2 cut(s) 769, 1363
HindIII AAGCTT 2 cut(s) 879, 1252
HinfI GANTC 8 cut(s) 426, 454, 511, 765, 920, 1186, 1305, 1533
HpaI GTTAAC 1 cut(s) 1363
HpaII CCGG 6 cut(s) 6, 71, 128, 873, 1077, 1131
HphI GGTGA 2 cut(s) 85, 887
Hpy166II GTNNAC 6 cut(s) 769, 924, 1031, 1353, 1363, 1381
Hpy188I TCNGA 6 cut(s) 249, 462, 777, 1286, 1304, 1552
Hpy188III TCNNGA 6 cut(s) 267, 398, 508, 595, 715, 1466
Hpy8I GTNNAC 6 cut(s) 769, 924, 1031, 1353, 1363, 1381
Hpy99I CGWCG 1 cut(s) 1501
HpyAV CCTTC 4 cut(s) 226, 629, 987, 1104
HpyCH4III ACNGT 6 cut(s) 181, 569, 639, 739, 1338, 1493
HpyCH4IV ACGT 1 cut(s) 1233
HpyCH4V TGCA 5 cut(s) 105, 286, 524, 591, 1229
HpyF10VI GCNNNNNNNGC 5 cut(s) 810, 878, 893, 1178, 1442
HpyF3I CTNAG 7 cut(s) 160, 396, 509, 609, 774, 986, 1250
HpySE526I ACGT 1 cut(s) 1233
Hsp92II CATG 6 cut(s) 53, 421, 825, 872, 1321, 1540
HspAI GCGC 5 cut(s) 35, 490, 725, 960, 1088
Ksp22I TGATCA 1 cut(s) 1410
KspAI GTTAAC 1 cut(s) 1363
Kzo9I GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
LmnI GCTCC 6 cut(s) 510, 746, 1040, 1052, 1169, 1198
Lsp1109I GCAGC 1 cut(s) 1217
LweI GCATC 5 cut(s) 114, 156, 600, 736, 1064
MaeI CTAG 5 cut(s) 303, 533, 542, 581, 1557
MaeII ACGT 1 cut(s) 1233
MaeIII GTNAC 1 cut(s) 1471
MalI GATC 8 cut(s) 81, 393, 416, 464, 614, 795, 1412, 1530
MboI GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
MboII GAAGA 8 cut(s) 81, 239, 449, 667, 1013, 1306, 1419, 1577
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 439, 567
MluCI AATT 4 cut(s) 404, 915, 1163, 1292
MluI ACGCGT 1 cut(s) 1096
MlyI GAGTC 3 cut(s) 520, 774, 929
MmeI TCCRAC 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 593
MseI TTAA 3 cut(s) 273, 408, 1362
MslI CAYNNNNRTG 2 cut(s) 873, 941
MspA1I CMGCKG 3 cut(s) 314, 480, 1358
MspI CCGG 6 cut(s) 6, 71, 128, 873, 1077, 1131
MspR9I CCNGG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 1457
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 2 cut(s) 725, 1098
MwoI GCNNNNNNNGC 5 cut(s) 810, 878, 893, 1178, 1442
NcoI CCATGG 1 cut(s) 821
NdeII GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
NheI GCTAGC 1 cut(s) 580
NlaIII CATG 6 cut(s) 53, 421, 825, 872, 1321, 1540
NlaIV GGNNCC 4 cut(s) 393, 506, 748, 855
NsiI ATGCAT 1 cut(s) 593
PctI GAATGC 1 cut(s) 1457
PfeI GAWTC 5 cut(s) 426, 454, 1186, 1305, 1533
PkrI GCNGC 4 cut(s) 39, 42, 815, 1207
PleI GAGTC 3 cut(s) 519, 773, 928
PpsI GAGTC 3 cut(s) 519, 773, 928
PpuMI RGGWCCY 1 cut(s) 854
Psp5II RGGWCCY 1 cut(s) 854
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 1445
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 4 cut(s) 393, 506, 748, 855
PspPI GGNCC 2 cut(s) 854, 1373
PspPPI RGGWCCY 1 cut(s) 854
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 4 cut(s) 342, 377, 641, 1340
RsaNI GTAC 4 cut(s) 341, 376, 640, 1339
RseI CAYNNNNRTG 2 cut(s) 873, 941
SaqAI TTAA 3 cut(s) 273, 408, 1362
SatI GCNGC 4 cut(s) 38, 41, 814, 1206
Sau3AI GATC 8 cut(s) 79, 391, 414, 462, 612, 793, 1410, 1528
Sau96I GGNCC 2 cut(s) 854, 1373
ScaI AGTACT 1 cut(s) 1340
SchI GAGTC 3 cut(s) 520, 774, 929
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 2 cut(s) 439, 567
SfaNI GCATC 5 cut(s) 114, 156, 600, 736, 1064
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 2 cut(s) 854, 1373
SmiMI CAYNNNNRTG 2 cut(s) 873, 941
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 4 cut(s) 404, 915, 1163, 1292
SsiI CCGC 7 cut(s) 38, 41, 314, 480, 813, 968, 974
SspMI CTAG 5 cut(s) 303, 533, 542, 581, 1557
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 122, 821
TaaI ACNGT 6 cut(s) 181, 569, 639, 739, 1338, 1493
TaiI ACGT 1 cut(s) 1236
TaqI TCGA 8 cut(s) 452, 594, 658, 682, 947, 1184, 1328, 1531
TaqII GACCGA 2 cut(s) 1300, 1524
TasI AATT 4 cut(s) 404, 915, 1163, 1292
TatI WGTACW 2 cut(s) 639, 1338
TauI GCSGC 3 cut(s) 40, 43, 816
TfiI GAWTC 5 cut(s) 426, 454, 1186, 1305, 1533
Tru1I TTAA 3 cut(s) 273, 408, 1362
Tru9I TTAA 3 cut(s) 273, 408, 1362
TscAI CASTG 1 cut(s) 1498
TseI GCWGC 1 cut(s) 1205
TspDTI ATGAA 6 cut(s) 240, 857, 891, 1285, 1306, 1525
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 1 cut(s) 1498
VpaK11BI GGWCC 2 cut(s) 854, 1373
XapI RAATTY 3 cut(s) 404, 1163, 1292
XmiI GTMKAC 1 cut(s) 1352
XspI CTAG 5 cut(s) 303, 533, 542, 581, 1557
ZrmI AGTACT 1 cut(s) 1340
Zsp2I ATGCAT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.