Rh4BG424300

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005215
Physical Location & Seq
Forward (+)
56850439 .. 56855617
5179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG424300.1

Sequence Viewer

Length: 1206 bp
ATGGCAGGAGGTGGACATGCACCACCGCCGAAACAGGAGGAGTTACAGCCTCATCCAGTAAAAGATCAGCTACCAAACGTTTCCTACTGCATTACCAGTCCTCCTCCTTGGCCTGAGGCTATCCTACTTGGTTTCCAACATTACTTGGTGATGCTTGGCACAGCAGTTCTCATTCCCACCTATCTTGTTCCTCAGATGGGAGGAGGAAATGAGGAGAAAGCAAAAATGATCCAGACTATACTGTTTGTTGCTGGAATCAACACATTGCAGCAGACATTTTTTGGGACTCGTTTACCTGCAGTAATTGGAGCATCTTATACCTATGTCCCAACAACCATTTCTATTATCTTGGCTGGTCGATATAGTGATGTTGTGAATCCCCAGGAGAAATTCGAGAAAATAATGCGTGGAACCCAGGGTGCACTCATTGTTGCCTCTATTCTGCAGATTGTTGTTGGTTTCAGTGGCCTTTGGCGCAATGTGGCGAGGTTCCAAAGTCCACTTTCTACTGTTCCTTTGGTTGCTTTGTCAGGCTTTGGCCTATATGAGCTTGGTTTTCCTGTGTTTGCAAAATGTGTGGAGATTGGGCTACCGCAACTCATTCTTCTACTGATATTTTCACAGTACATACCTCATCTGATGCGCAGTGAGACGCATGTCTTTGATCGCTTTGCTGTTTTATTTTCGGTGACAATTGTGTGGATTTATGCTCATCTGCTCACTGTGGGCGGGGCTTATAAGAACACACCAGAAGCAACTCAGTTAACCTGTCGAACTGATCGTGCTGGCATCATACATGCTGCTCCATGGATAAGAGTTCCATATCCTTTCCAATGGGGAGCTCCCACTTTTGATGCTGGAGAAGCTTTTGCAATGATGACTGCTTCATTTGTTGCTCTTGTAGAGTCCACCGGTGTCTTTATTGCTGTGTCAAGGTATGCAAGTGCAACTCCACTCCCACCTGCTATTTTAAGTCGCGGTATTGGTTGGCAGGGAGTGGGCATTCTTTTGTCTGGTATATTCGGCACTGGGAATGGATCATCAGTATCTGTTGAAAATGCTGGTTTGTTAGCTTTGACTCGAGTTGGTAGCCGAAGGGTTGTCCAAATTTCAGCCAGCTTCATGATCTTCTTTTCCATACTTGGTAGGTTATTCATAGTTTCATACTGTGAATTCCCTCATTCTTTCTCCTTTTGCAAGTGTTAA

Protein Analysis

401

Amino Acids

43.52

Weight (kDa)

8.86

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 383 3.7e-50 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AarI CACCTGC 1 cut(s) 970
Acc16I TGCGCA 1 cut(s) 644
Acc36I ACCTGC 2 cut(s) 304, 970
AccII CGCG 1 cut(s) 978
AciI CCGC 4 cut(s) 26, 593, 729, 978
AclI AACGTT 1 cut(s) 78
AclWI GGATC 2 cut(s) 223, 1045
AcsI RAATTY 3 cut(s) 389, 1107, 1172
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 911
AgsI TTSAA 1 cut(s) 1055
AjnI CCWGG 2 cut(s) 381, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 6 cut(s) 70, 550, 842, 866, 1073, 1119
AluI AGCT 6 cut(s) 70, 550, 842, 866, 1073, 1119
Alw21I GWGCWC 2 cut(s) 424, 844
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 1 cut(s) 420
AlwI GGATC 2 cut(s) 223, 1045
AlwNI CAGNNNCTG 1 cut(s) 1049
Ama87I CYCGRG 1 cut(s) 1080
AoxI GGCC 3 cut(s) 110, 466, 538
ApaLI GTGCAC 1 cut(s) 420
ApeKI GCWGC 2 cut(s) 268, 800
ApoI RAATTY 3 cut(s) 389, 1107, 1172
AsiGI ACCGGT 1 cut(s) 911
AspLEI GCGC 2 cut(s) 477, 645
AsuHPI GGTGA 2 cut(s) 160, 700
AvaI CYCGRG 1 cut(s) 1080
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 424
BanII GRGCYC 1 cut(s) 844
Bbv12I GWGCWC 2 cut(s) 424, 844
BbvI GCAGC 2 cut(s) 280, 787
BccI CCATC 1 cut(s) 190
BciT130I CCWGG 2 cut(s) 383, 416
BcoDI GTCTC 1 cut(s) 644
BfmI CTRYAG 2 cut(s) 297, 443
BfuAI ACCTGC 2 cut(s) 304, 970
BisI GCNGC 2 cut(s) 269, 801
BlsI GCNGC 2 cut(s) 270, 802
Bme1390I CCNGG 2 cut(s) 383, 416
BmeT110I CYCGRG 1 cut(s) 1080
BmiI GGNNCC 2 cut(s) 412, 491
BmrFI CCNGG 2 cut(s) 383, 416
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 5 cut(s) 141, 320, 630, 798, 844
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 656
BpmI CTGGAG 1 cut(s) 879
BsaJI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 3 cut(s) 56, 96, 1033
Bse21I CCTNAGG 1 cut(s) 114
Bse3DI GCAATG 3 cut(s) 263, 484, 879
BseBI CCWGG 2 cut(s) 383, 416
BseDI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 3 cut(s) 263, 484, 879
BseMII CTCAG 3 cut(s) 105, 206, 773
BseNI ACTGG 3 cut(s) 56, 96, 1033
BseRI GAGGAG 4 cut(s) 53, 93, 216, 227
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 2 cut(s) 280, 787
Bsh1236I CGCG 1 cut(s) 978
BshFI GGCC 3 cut(s) 112, 468, 540
BshTI ACCGGT 1 cut(s) 911
BsiHKAI GWGCWC 2 cut(s) 424, 844
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 1 cut(s) 912
BslFI GGGAC 2 cut(s) 298, 311
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 644
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 2 cut(s) 298, 311
BsmI GAATGC 1 cut(s) 1002
BsnI GGCC 3 cut(s) 112, 468, 540
BsoBI CYCGRG 1 cut(s) 1080
Bsp1286I GDGCHC 2 cut(s) 424, 844
Bsp143I GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 4 cut(s) 26, 593, 729, 978
BspANI GGCC 3 cut(s) 112, 468, 540
BspCNI CTCAG 3 cut(s) 106, 205, 772
BspFNI CGCG 1 cut(s) 978
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 2 cut(s) 412, 491
BspMAI CTGCAG 2 cut(s) 301, 447
BspMI ACCTGC 2 cut(s) 304, 970
BspPI GGATC 2 cut(s) 223, 1045
BsrDI GCAATG 3 cut(s) 263, 484, 879
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 3 cut(s) 56, 96, 1033
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 5 cut(s) 107, 381, 414, 415, 806
BssMI GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
BssT1I CCWWGG 2 cut(s) 107, 806
Bst2UI CCWGG 2 cut(s) 383, 416
Bst4CI ACNGT 5 cut(s) 243, 511, 624, 724, 1169
BstC8I GCNNGC 2 cut(s) 787, 1117
BstDEI CTNAG 3 cut(s) 114, 192, 759
BstDSI CCRYGG 1 cut(s) 806
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 1 cut(s) 978
BstHHI GCGC 2 cut(s) 477, 645
BstKTI GATC 6 cut(s) 67, 231, 667, 781, 1040, 1128
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
BstMWI GCNNNNNNNGC 2 cut(s) 474, 863
BstNI CCWGG 2 cut(s) 383, 416
BstNSI RCATGY 3 cut(s) 20, 659, 800
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 381, 414
BstSFI CTRYAG 2 cut(s) 297, 443
BstSLI GKGCMC 1 cut(s) 424
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 2 cut(s) 280, 787
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 3 cut(s) 112, 468, 540
BtgI CCRYGG 1 cut(s) 806
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 4 cut(s) 469, 652, 720, 1026
BveI ACCTGC 2 cut(s) 304, 970
Cac8I GCNNGC 2 cut(s) 787, 1117
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 2 cut(s) 477, 645
Cfr10I RCCGGY 1 cut(s) 911
CseI GACGC 1 cut(s) 661
Csp6I GTAC 1 cut(s) 625
CspAI ACCGGT 1 cut(s) 911
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 558, 593
CviAII CATG 5 cut(s) 17, 656, 797, 807, 1123
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 3 cut(s) 114, 192, 759
DpnI GATC 6 cut(s) 66, 230, 666, 780, 1039, 1127
DpnII GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
Ecl136II GAGCTC 1 cut(s) 842
Eco130I CCWWGG 2 cut(s) 107, 806
Eco24I GRGCYC 1 cut(s) 844
Eco53kI GAGCTC 1 cut(s) 842
Eco81I CCTNAGG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 1080
EcoICRI GAGCTC 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 1172
EcoRII CCWGG 2 cut(s) 381, 414
EcoT14I CCWWGG 2 cut(s) 107, 806
EcoT38I GRGCYC 1 cut(s) 844
ErhI CCWWGG 2 cut(s) 107, 806
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 5 cut(s) 20, 659, 800, 810, 1126
FaqI GGGAC 2 cut(s) 298, 311
FatI CATG 5 cut(s) 16, 655, 796, 806, 1122
FauI CCCGC 1 cut(s) 722
Fnu4HI GCNGC 2 cut(s) 269, 801
FokI GGATG 1 cut(s) 39
FriOI GRGCYC 1 cut(s) 844
Fsp4HI GCNGC 2 cut(s) 269, 801
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 2 cut(s) 476, 644
GluI GCNGC 2 cut(s) 269, 801
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 3 cut(s) 112, 468, 540
HapII CCGG 1 cut(s) 912
HgaI GACGC 1 cut(s) 661
HhaI GCGC 2 cut(s) 477, 645
Hin1II CATG 5 cut(s) 20, 659, 800, 810, 1126
Hin6I GCGC 2 cut(s) 475, 643
HinP1I GCGC 2 cut(s) 475, 643
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 5 cut(s) 255, 286, 376, 905, 1078
HpaI GTTAAC 1 cut(s) 765
HpaII CCGG 1 cut(s) 912
HphI GGTGA 2 cut(s) 160, 700
Hpy166II GTNNAC 6 cut(s) 14, 293, 422, 500, 765, 909
Hpy188I TCNGA 2 cut(s) 195, 639
Hpy188III TCNNGA 3 cut(s) 232, 394, 1123
Hpy8I GTNNAC 6 cut(s) 14, 293, 422, 500, 765, 909
HpyAV CCTTC 1 cut(s) 1089
HpyCH4III ACNGT 5 cut(s) 243, 511, 624, 724, 1169
HpyCH4IV ACGT 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 2 cut(s) 474, 863
HpyF3I CTNAG 3 cut(s) 114, 192, 759
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 5 cut(s) 20, 659, 800, 810, 1126
HspAI GCGC 2 cut(s) 475, 643
KspAI GTTAAC 1 cut(s) 765
Kzo9I GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
LmnI GCTCC 4 cut(s) 308, 808, 839, 847
Lsp1109I GCAGC 2 cut(s) 280, 787
LweI GCATC 5 cut(s) 141, 320, 630, 798, 844
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 42, 688
MalI GATC 6 cut(s) 66, 230, 666, 780, 1039, 1127
MboI GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
MboII GAAGA 2 cut(s) 596, 1120
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 2 cut(s) 424, 844
MluCI AATT 5 cut(s) 303, 389, 693, 1107, 1172
MlyI GAGTC 3 cut(s) 280, 914, 1072
MmeI TCCRAC 1 cut(s) 160
MseI TTAA 3 cut(s) 764, 971, 1204
MspI CCGG 1 cut(s) 912
MspR9I CCNGG 2 cut(s) 383, 416
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 1002
MvaI CCWGG 2 cut(s) 383, 416
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 2 cut(s) 474, 863
NcoI CCATGG 1 cut(s) 806
NdeII GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
NlaIII CATG 5 cut(s) 20, 659, 800, 810, 1126
NlaIV GGNNCC 2 cut(s) 412, 491
NmuCI GTSAC 1 cut(s) 688
NsbI TGCGCA 1 cut(s) 644
NspI RCATGY 3 cut(s) 20, 659, 800
PaeR7I CTCGAG 1 cut(s) 1080
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 970
PasI CCCWGGG 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PctI GAATGC 1 cut(s) 1002
PfeI GAWTC 2 cut(s) 255, 376
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 2 cut(s) 270, 802
PleI GAGTC 3 cut(s) 280, 913, 1072
PpsI GAGTC 3 cut(s) 280, 913, 1072
PshAI GACNNNNGTC 1 cut(s) 656
PsiI TTATAA 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 844
Psp1406I AACGTT 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 381, 414
PspGI CCWGG 2 cut(s) 381, 414
PspN4I GGNNCC 2 cut(s) 412, 491
PspXI VCTCGAGB 1 cut(s) 1080
PstI CTGCAG 2 cut(s) 301, 447
PstNI CAGNNNCTG 1 cut(s) 1049
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
SacI GAGCTC 1 cut(s) 844
SaqAI TTAA 3 cut(s) 764, 971, 1204
SatI GCNGC 2 cut(s) 269, 801
Sau3AI GATC 6 cut(s) 64, 228, 664, 778, 1037, 1125
SchI GAGTC 3 cut(s) 280, 914, 1072
ScrFI CCNGG 2 cut(s) 383, 416
SduI GDGCHC 2 cut(s) 424, 844
SfaNI GCATC 5 cut(s) 141, 320, 630, 798, 844
SfcI CTRYAG 2 cut(s) 297, 443
Sfr274I CTCGAG 1 cut(s) 1080
SgrAI CRCCGGYG 1 cut(s) 911
SlaI CTCGAG 1 cut(s) 1080
SmlI CTYRAG 1 cut(s) 1080
SmoI CTYRAG 1 cut(s) 1080
Sse9I AATT 5 cut(s) 303, 389, 693, 1107, 1172
SsiI CCGC 4 cut(s) 26, 593, 729, 978
SstI GAGCTC 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 381, 414
StyI CCWWGG 2 cut(s) 107, 806
TaaI ACNGT 5 cut(s) 243, 511, 624, 724, 1169
TaiI ACGT 1 cut(s) 81
TaqI TCGA 4 cut(s) 358, 393, 772, 1081
TasI AATT 5 cut(s) 303, 389, 693, 1107, 1172
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 2 cut(s) 255, 376
Tru1I TTAA 3 cut(s) 764, 971, 1204
Tru9I TTAA 3 cut(s) 764, 971, 1204
TscAI CASTG 4 cut(s) 469, 652, 727, 1033
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 2 cut(s) 268, 800
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 876, 1111, 1144, 1152
TspRI CASTG 4 cut(s) 469, 652, 727, 1033
VneI GTGCAC 1 cut(s) 420
XapI RAATTY 3 cut(s) 389, 1107, 1172
XceI RCATGY 3 cut(s) 20, 659, 800
XhoI CTCGAG 1 cut(s) 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.