MD16G1088000.v1.1

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005215
Physical Location & Seq
Reverse (-)
6070103 .. 6074247
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1088000.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGGCAGGAGGTGGACATGCACCGCCGCCGAAACAGGAGGAGCTACAGCCTCATCCGGCCAAAGATCAGCTGCCAAACATTTCCTACTGCATTACCAGTCCTCCTCCTTGGCCTGAAGCCATCCTACTTGGTTTCCAACATTACTTGGTGATGCTTGGCACAACGGTTCTTATTCCCACGTTCCTTGTTCCTCAGATGGGAGGAGGAAATGGGGAGAAAGCGAAAATGATTCAGACTATACTGTTTGTTGCTGGATTAAACACACTGCTTCAAACATTCTTAGGGACTCGTTTACCTGCAGTGATTGGAGCATCTTATACCTACGTGCCAACAACCATTTCGATTATCTTGGCTGGTCGGTATAGTGACATTGTGAATCCCCAGGAGAAATTTGAGAAGATAATGCGTGGAACCCAGGGTGCACTTATTGTTGCCTCAACTCTGCAGATTGTTGTTGGCTTCAGTGGCCTTTGGCGCAATATGGCAAGGTTCCTAAGTCCGCTTGCTGCTGTTCCTTTGGTTGCTTTGTCAGGCTTTGGGCTATACGAGTTTGGTTTTCCTGTGTTTGCAAAATGCGTGGAGATTGGACTACCGCAACTCATCATGTTACTGATATTTTCTCAGTACATACCTCACTTAATGCGCAGTGAAACACACGTCTTTGATCGCTTTGCTGTTTTATTTTCGGTGACCATTGTGTGGATTTACGCTCACCTGCTTACTGTGGGTGGGGCCTATAAGAACACAGCACCCACAACTCAATTAACCTGTCGAACTGATCGTGCTGGTATTATAGGTGCTGCTCCATGGATAAGAGTTCCATATCCCTTTCAATGGGGTGCTCCCACATTCGATGCTGGAGAAGCTTTTGCAATGATGGCTGTTTCATTTGTTGCCCTTGTAGAGTCCACCGGTGCCTTCATTGCTGTGGCAAGGTATGCAAGTGCAACTCCACTGCCGCCTTCTATTTTAAGCCGCGGTGTTGGTTGGCAGGGAGTGGGAATTCTTTTCTGTGGTATATTCGGCACTGGGAATGGAGCTTCAGTATCTGTTGAAAATGCTGGTTTGTTAGCATTGACACGGGTTGGTAGCCGAAGGGTGGTTCAAATATCAGCCTGTTTCATGATCTTCTTTTCCATCCTTGGAAAATTTGGAGCTGTCTTTGCCTCAATTCCGGCACCCATCATTGCAGCATCGTATTGCTTTTTCTTCGCTTATGTCGGTTCAGCAGGTCTTACCTTTCTTCAATTTTGCAACCTCAACAGTTTTAGGACAAAGTTTATCTTAGGCTTCTCGATTTACATGGGCTTGTCGATTCCACAATATTTCAACGAATACACCCTCATTAAAGGCTACGGCCCGGTGCACACTGGAGCAAGATGGTTCAATGATATGATCAACGTTCCTTTCTCTTCCGAACCATTTGTTGCCGGTTTCTTGGCGATGTTCTTGGACATCACGCTACACCGCAAGGACAACGCGACAAGGAAAGACCGTGGCATGCATTGGTGGGATAGGTTCCGATCTTTCAAGACGGATACTAGAAGCGAGGAATTCTACTCCCTACCTTTCAACCTTAACAAGTTTTTCCCCTCGGTGTGA

Protein Analysis

534

Amino Acids

58.48

Weight (kDa)

9.29

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 40 - 443 6.4e-64 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 723
Acc16I TGCGCA 1 cut(s) 644
Acc36I ACCTGC 3 cut(s) 304, 723, 1220
AccB1I GGYRCC 2 cut(s) 914, 1177
AccB7I CCANNNNNTGG 1 cut(s) 699
AccII CGCG 2 cut(s) 978, 1481
AciI CCGC 8 cut(s) 23, 26, 500, 593, 959, 976, 978, 1468
AclI AACGTT 1 cut(s) 1401
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 4 cut(s) 389, 1002, 1148, 1553
AcuI CTGAAG 3 cut(s) 135, 445, 1026
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 4 cut(s) 197, 699, 834, 1099
AflIII ACRYGT 1 cut(s) 655
AgeI ACCGGT 1 cut(s) 911
AgsI TTSAA 9 cut(s) 272, 833, 1055, 1106, 1247, 1330, 1387, 1531, 1573
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 2 cut(s) 381, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 5 cut(s) 43, 70, 866, 1040, 1157
AluI AGCT 5 cut(s) 43, 70, 866, 1040, 1157
Alw21I GWGCWC 3 cut(s) 424, 844, 1368
Alw44I GTGCAC 2 cut(s) 420, 1364
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 5 cut(s) 57, 110, 466, 732, 1357
ApaLI GTGCAC 2 cut(s) 420, 1364
ApeKI GCWGC 4 cut(s) 70, 506, 800, 1190
ApoI RAATTY 4 cut(s) 389, 1002, 1148, 1553
AsiGI ACCGGT 1 cut(s) 911
AspLEI GCGC 2 cut(s) 477, 645
AspS9I GGNCC 2 cut(s) 732, 1358
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 3 cut(s) 160, 700, 704
BaeGI GKGCMC 2 cut(s) 424, 1368
BanI GGYRCC 2 cut(s) 914, 1177
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
Bbv12I GWGCWC 3 cut(s) 424, 844, 1368
BbvI GCAGC 4 cut(s) 57, 493, 787, 1202
BccI CCATC 6 cut(s) 128, 190, 871, 1145, 1190, 1374
BceAI ACGGC 1 cut(s) 1372
BciT130I CCWGG 2 cut(s) 383, 416
BciVI GTATCC 1 cut(s) 1531
BclI TGATCA 1 cut(s) 1395
BcnI CCSGG 1 cut(s) 1361
BfaI CTAG 1 cut(s) 1542
BfmI CTRYAG 3 cut(s) 44, 297, 443
BfuAI ACCTGC 3 cut(s) 304, 723, 1220
BfuI GTATCC 1 cut(s) 1531
BisI GCNGC 7 cut(s) 26, 71, 507, 801, 959, 976, 1191
BlsI GCNGC 7 cut(s) 27, 72, 508, 802, 960, 977, 1192
Bme1390I CCNGG 3 cut(s) 383, 416, 1361
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 2 cut(s) 732, 1358
BmiI GGNNCC 6 cut(s) 412, 491, 733, 916, 1179, 1520
BmrFI CCNGG 3 cut(s) 383, 416, 1361
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 4 cut(s) 141, 320, 844, 1202
BmuI ACTGGG 1 cut(s) 1038
BpmI CTGGAG 2 cut(s) 879, 1392
BpuMI CCSGG 1 cut(s) 1361
BsaAI YACGTR 1 cut(s) 325
BsaJI CCNNGG 9 cut(s) 107, 381, 414, 415, 806, 976, 1141, 1495, 1593
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 1029, 1059
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 699, 834, 1099
Bse118I RCCGGY 2 cut(s) 911, 1430
Bse1I ACTGG 3 cut(s) 96, 1033, 1375
Bse3DI GCAATG 3 cut(s) 879, 921, 1185
BseBI CCWGG 2 cut(s) 383, 416
BseDI CCNNGG 9 cut(s) 107, 381, 414, 415, 806, 976, 1141, 1495, 1593
BseGI GGATG 3 cut(s) 52, 120, 1137
BseLI CCNNNNNNNGG 4 cut(s) 197, 699, 834, 1099
BseMI GCAATG 3 cut(s) 879, 921, 1185
BseMII CTCAG 2 cut(s) 206, 635
BseNI ACTGG 3 cut(s) 96, 1033, 1375
BseRI GAGGAG 3 cut(s) 53, 93, 216
BseSI GKGCMC 2 cut(s) 424, 1368
BseXI GCAGC 4 cut(s) 57, 493, 787, 1202
Bsh1236I CGCG 2 cut(s) 978, 1481
BshFI GGCC 5 cut(s) 59, 112, 468, 734, 1359
BshNI GGYRCC 2 cut(s) 914, 1177
BshTI ACCGGT 1 cut(s) 911
BsiHKAI GWGCWC 3 cut(s) 424, 844, 1368
BsiSI CCGG 5 cut(s) 56, 912, 1175, 1361, 1431
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 4 cut(s) 197, 699, 834, 1099
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 5 cut(s) 59, 112, 468, 734, 1359
Bsp1286I GDGCHC 3 cut(s) 424, 844, 1368
Bsp143I GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 8 cut(s) 23, 26, 500, 593, 959, 976, 978, 1468
BspANI GGCC 5 cut(s) 59, 112, 468, 734, 1359
BspCNI CTCAG 2 cut(s) 205, 634
BspFNI CGCG 2 cut(s) 978, 1481
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 6 cut(s) 412, 491, 733, 916, 1179, 1520
BspMAI CTGCAG 2 cut(s) 301, 447
BspMI ACCTGC 3 cut(s) 304, 723, 1220
BspT107I GGYRCC 2 cut(s) 914, 1177
BsrDI GCAATG 3 cut(s) 879, 921, 1185
BsrFI RCCGGY 2 cut(s) 911, 1430
BsrI ACTGG 3 cut(s) 96, 1033, 1375
BssAI RCCGGY 2 cut(s) 911, 1430
BssECI CCNNGG 9 cut(s) 107, 381, 414, 415, 806, 976, 1141, 1495, 1593
BssMI GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
BssT1I CCWWGG 3 cut(s) 107, 806, 1141
Bst2UI CCWGG 2 cut(s) 383, 416
Bst4CI ACNGT 5 cut(s) 166, 243, 724, 1265, 1496
Bst6I CTCTTC 1 cut(s) 1417
BstAPI GCANNNNNTGC 1 cut(s) 938
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 2 cut(s) 504, 1502
BstDEI CTNAG 5 cut(s) 192, 280, 494, 621, 1285
BstDSI CCRYGG 3 cut(s) 806, 976, 1495
BstEII GGTNACC 1 cut(s) 688
BstF5I GGATG 3 cut(s) 52, 120, 1137
BstFNI CGCG 2 cut(s) 978, 1481
BstHHI GCGC 2 cut(s) 477, 645
BstKTI GATC 6 cut(s) 67, 667, 781, 1128, 1398, 1526
BstMBI GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
BstMWI GCNNNNNNNGC 7 cut(s) 465, 474, 863, 878, 923, 938, 1163
BstNI CCWGG 2 cut(s) 383, 416
BstNSI RCATGY 2 cut(s) 20, 1504
BstPI GGTNACC 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 381, 414, 1359
BstSFI CTRYAG 3 cut(s) 44, 297, 443
BstSLI GKGCMC 2 cut(s) 424, 1368
BstUI CGCG 2 cut(s) 978, 1481
BstV1I GCAGC 4 cut(s) 57, 493, 787, 1202
BsuI GTATCC 1 cut(s) 1531
BsuRI GGCC 5 cut(s) 59, 112, 468, 734, 1359
BtgI CCRYGG 3 cut(s) 806, 976, 1495
BtgZI GCGATG 1 cut(s) 1457
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 3 cut(s) 52, 120, 1137
BtsI GCAGTG 4 cut(s) 263, 306, 652, 953
BtsIMutI CAGTG 7 cut(s) 263, 306, 469, 652, 953, 1026, 1368
BveI ACCTGC 3 cut(s) 304, 723, 1220
Cac8I GCNNGC 2 cut(s) 504, 1502
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 2 cut(s) 477, 645
Cfr10I RCCGGY 2 cut(s) 911, 1430
Cfr13I GGNCC 2 cut(s) 732, 1358
Cfr42I CCGCGG 1 cut(s) 979
Csp6I GTAC 1 cut(s) 625
CspAI ACCGGT 1 cut(s) 911
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 558, 593
CviAII CATG 6 cut(s) 17, 604, 807, 1123, 1303, 1501
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 5 cut(s) 192, 280, 494, 621, 1285
DpnI GATC 6 cut(s) 66, 666, 780, 1127, 1397, 1525
DpnII GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 1417
EarI CTCTTC 1 cut(s) 1417
Eco130I CCWWGG 3 cut(s) 107, 806, 1141
Eco57I CTGAAG 3 cut(s) 135, 445, 1026
Eco91I GGTNACC 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 732
EcoO65I GGTNACC 1 cut(s) 688
EcoRI GAATTC 2 cut(s) 1002, 1553
EcoRII CCWGG 2 cut(s) 381, 414
EcoT14I CCWWGG 3 cut(s) 107, 806, 1141
EcoT22I ATGCAT 1 cut(s) 1506
ErhI CCWWGG 3 cut(s) 107, 806, 1141
FaeI CATG 6 cut(s) 20, 607, 810, 1126, 1306, 1504
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FaqI GGGAC 1 cut(s) 298
FatI CATG 6 cut(s) 16, 603, 806, 1122, 1302, 1500
FbaI TGATCA 1 cut(s) 1395
Fnu4HI GCNGC 7 cut(s) 26, 71, 507, 801, 959, 976, 1191
FokI GGATG 3 cut(s) 39, 107, 1124
Fsp4HI GCNGC 7 cut(s) 26, 71, 507, 801, 959, 976, 1191
FspBI CTAG 1 cut(s) 1542
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 2 cut(s) 476, 644
GluI GCNGC 7 cut(s) 26, 71, 507, 801, 959, 976, 1191
GsuI CTGGAG 2 cut(s) 879, 1392
HaeIII GGCC 5 cut(s) 59, 112, 468, 734, 1359
HapII CCGG 5 cut(s) 56, 912, 1175, 1361, 1431
HhaI GCGC 2 cut(s) 477, 645
Hin1II CATG 6 cut(s) 20, 607, 810, 1126, 1306, 1504
Hin6I GCGC 2 cut(s) 475, 643
HinP1I GCGC 2 cut(s) 475, 643
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 5 cut(s) 229, 286, 376, 905, 1315
HpaII CCGG 5 cut(s) 56, 912, 1175, 1361, 1431
HphI GGTGA 3 cut(s) 160, 700, 704
Hpy166II GTNNAC 5 cut(s) 14, 293, 422, 909, 1366
Hpy188I TCNGA 4 cut(s) 195, 234, 1417, 1523
Hpy188III TCNNGA 3 cut(s) 1123, 1294, 1531
Hpy8I GTNNAC 5 cut(s) 14, 293, 422, 909, 1366
HpyAV CCTTC 3 cut(s) 928, 972, 1089
HpyCH4III ACNGT 5 cut(s) 166, 243, 724, 1265, 1496
HpyCH4IV ACGT 4 cut(s) 179, 324, 657, 1401
HpyF10VI GCNNNNNNNGC 7 cut(s) 465, 474, 863, 878, 923, 938, 1163
HpyF3I CTNAG 5 cut(s) 192, 280, 494, 621, 1285
HpySE526I ACGT 4 cut(s) 179, 324, 657, 1401
Hsp92II CATG 6 cut(s) 20, 607, 810, 1126, 1306, 1504
HspAI GCGC 2 cut(s) 475, 643
Ksp22I TGATCA 1 cut(s) 1395
KspI CCGCGG 1 cut(s) 979
Kzo9I GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
LmnI GCTCC 7 cut(s) 40, 308, 808, 847, 1037, 1154, 1373
Lsp1109I GCAGC 4 cut(s) 57, 493, 787, 1202
LweI GCATC 4 cut(s) 141, 320, 844, 1202
MaeI CTAG 1 cut(s) 1542
MaeII ACGT 4 cut(s) 179, 324, 657, 1401
MaeIII GTNAC 3 cut(s) 365, 606, 688
MalI GATC 6 cut(s) 66, 666, 780, 1127, 1397, 1525
MboI GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
MboII GAAGA 5 cut(s) 409, 1120, 1201, 1235, 1404
MhlI GDGCHC 3 cut(s) 424, 844, 1368
MluCI AATT 7 cut(s) 389, 761, 1002, 1148, 1170, 1247, 1553
MlyI GAGTC 2 cut(s) 280, 914
MmeI TCCRAC 1 cut(s) 160
Mph1103I ATGCAT 1 cut(s) 1506
MseI TTAA 6 cut(s) 257, 638, 764, 971, 1347, 1578
MslI CAYNNNNRTG 1 cut(s) 926
MspA1I CMGCKG 2 cut(s) 70, 978
MspI CCGG 5 cut(s) 56, 912, 1175, 1361, 1431
MspR9I CCNGG 3 cut(s) 383, 416, 1361
MvaI CCWGG 2 cut(s) 383, 416
MvnI CGCG 2 cut(s) 978, 1481
MwoI GCNNNNNNNGC 7 cut(s) 465, 474, 863, 878, 923, 938, 1163
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 806
NdeII GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
NlaIII CATG 6 cut(s) 20, 607, 810, 1126, 1306, 1504
NlaIV GGNNCC 6 cut(s) 412, 491, 733, 916, 1179, 1520
NmuCI GTSAC 2 cut(s) 365, 688
NsbI TGCGCA 1 cut(s) 644
NsiI ATGCAT 1 cut(s) 1506
NspI RCATGY 2 cut(s) 20, 1504
PaeI GCATGC 1 cut(s) 1504
PagI TCATGA 1 cut(s) 1122
PaqCI CACCTGC 1 cut(s) 723
PasI CCCWGGG 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PfeI GAWTC 3 cut(s) 229, 376, 1315
PflMI CCANNNNNTGG 1 cut(s) 699
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 7 cut(s) 27, 72, 508, 802, 960, 977, 1192
PleI GAGTC 2 cut(s) 280, 913
PpsI GAGTC 2 cut(s) 280, 913
Ppu21I YACGTR 1 cut(s) 325
Psp1406I AACGTT 1 cut(s) 1401
Psp6I CCWGG 2 cut(s) 381, 414
PspEI GGTNACC 1 cut(s) 688
PspGI CCWGG 2 cut(s) 381, 414
PspN4I GGNNCC 6 cut(s) 412, 491, 733, 916, 1179, 1520
PspPI GGNCC 2 cut(s) 732, 1358
PstI CTGCAG 2 cut(s) 301, 447
PstNI CAGNNNCTG 1 cut(s) 1049
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 926
SacII CCGCGG 1 cut(s) 979
SaqAI TTAA 6 cut(s) 257, 638, 764, 971, 1347, 1578
SatI GCNGC 7 cut(s) 26, 71, 507, 801, 959, 976, 1191
Sau3AI GATC 6 cut(s) 64, 664, 778, 1125, 1395, 1523
Sau96I GGNCC 2 cut(s) 732, 1358
SchI GAGTC 2 cut(s) 280, 914
ScrFI CCNGG 3 cut(s) 383, 416, 1361
SduI GDGCHC 3 cut(s) 424, 844, 1368
SfaNI GCATC 4 cut(s) 141, 320, 844, 1202
SfcI CTRYAG 3 cut(s) 44, 297, 443
Sfr303I CCGCGG 1 cut(s) 979
SgrAI CRCCGGYG 1 cut(s) 911
SgrBI CCGCGG 1 cut(s) 979
SmiMI CAYNNNNRTG 1 cut(s) 926
SphI GCATGC 1 cut(s) 1504
Sse9I AATT 7 cut(s) 389, 761, 1002, 1148, 1170, 1247, 1553
SsiI CCGC 8 cut(s) 23, 26, 500, 593, 959, 976, 978, 1468
SspI AATATT 1 cut(s) 1325
SspMI CTAG 1 cut(s) 1542
StyD4I CCNGG 3 cut(s) 381, 414, 1359
StyI CCWWGG 3 cut(s) 107, 806, 1141
TaaI ACNGT 5 cut(s) 166, 243, 724, 1265, 1496
TaiI ACGT 4 cut(s) 182, 327, 660, 1404
TaqI TCGA 5 cut(s) 341, 772, 852, 1295, 1313
TasI AATT 7 cut(s) 389, 761, 1002, 1148, 1170, 1247, 1553
TatI WGTACW 1 cut(s) 624
TauI GCSGC 3 cut(s) 28, 961, 978
TfiI GAWTC 3 cut(s) 229, 376, 1315
Tru1I TTAA 6 cut(s) 257, 638, 764, 971, 1347, 1578
Tru9I TTAA 6 cut(s) 257, 638, 764, 971, 1347, 1578
TscAI CASTG 7 cut(s) 270, 306, 469, 652, 960, 1033, 1375
TseFI GTSAC 2 cut(s) 365, 688
TseI GCWGC 4 cut(s) 70, 506, 800, 1190
Tsp45I GTSAC 2 cut(s) 365, 688
TspDTI ATGAA 3 cut(s) 876, 910, 1111
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 7 cut(s) 270, 306, 469, 652, 960, 1033, 1375
Van91I CCANNNNNTGG 1 cut(s) 699
VneI GTGCAC 2 cut(s) 420, 1364
XapI RAATTY 4 cut(s) 389, 1002, 1148, 1553
XceI RCATGY 2 cut(s) 20, 1504
XspI CTAG 1 cut(s) 1542
Zsp2I ATGCAT 1 cut(s) 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.