AT1G11720

Starch synthase 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003232
Physical Location & Seq
Forward (+)
3951366 .. 3957044
5679 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G11720.1

Sequence Viewer

Length: 3129 bp
ATGATTTCTTATTTTCTTAACCAAGACTTTTCAAGGAAGAAGCAAGGAAGAATGGCTGCTTCAGGACCAAAAAGCTCAGGTCCCAGAGGTTTTGGGCGACGAACAACAGTAGGAAGTGCTCAGAAAAGAACTCAGAAGAAGAATGGTGAAAAAGATAGTAATGCCACTTCTACAGCAACAAACGAGGTTTCAGGGATTAGTAAGTTGCCCGCAGCTAAAGTGGATGTACAGAAGCAAAGCTCTGTTGTTTTGAATGAGAGAAATGTGTTAGATAGGTCGGATATTGAGGATGGAAGTGATCGTTTGGACAAGAAAACAACCGATGATGATGATTTGTTAGAACAAAAGTTAAAACTTGAAAGAGAGAATCTTCGTAGGAAGGAAATAGAAACGCTTGCAGCGGAAAATTTGGCGAGAGGTGATAGAATGTTTGTGTATCCCGTTATTGTGAAACCTGATGAAGACATAGAAGTGTTTCTCAACAGGAATCTGTCGACTCTGAATAACGAACCCGATGTTTTGATCATGGGGGCGTTTAACGAATGGAGATGGAAGTCTTTCACAAGGAGATTGGAAAAGACCTGGATCCATGAAGATTGGTTGTCATGTCTCCTTCATATCCCCAAAGAAGCGTATAAGATGGACTTCGTGTTTTTCAATGGGCAAAGTGTATATGACAACAATGACTCAAAAGATTTTTGTGTAGAGATAAAAGGTGGGATGGATAAAGTTGACTTTGAGAATTTTCTTCTAGAAGAGAAACTGCGAGAGCAAGAGAAGTTAGCCAAGGAAGAAGCTGAGAGGGAGAGGCAAAAAGAAGAGAAGAGAAGAATCGAAGCTCAAAAGGCTGCAATTGAAGCTGATAGAGCACAAGCAAAGGCGGAGACTCAGAAGAGACGTGAATTGCTTCAACCGGCTATTAAGAAAGCTGTAGTCTCGGCTGAGAATGTTTGGTACATTGAGCCGAGTGATTTCAAGGCTGAAGATACAGTGAAGCTATATTACAATAAAAGGTCAGGTCCTCTGACTAATTCCAAAGAACTGTGGTTACATGGAGGGTTTAATAATTGGGTTGATGGATTATCTATCGTTGTAAAGCTTGTTAATGCTGAGTTAAAGGATGTTGATCCAAAGAGCGGAAATTGGTGGTTCGCTGAAGTTGTAGTGCCTGGCGGTGCACTAGTCATTGACTGGGTCTTTGCTGATGGACCACCTAAAGGAGCGTTTCTGTATGACAATAATGGTTACCAAGACTTCCACGCACTTGTTCCTCAAAAACTTCCTGAAGAACTTTACTGGTTAGAGGAAGAAAATATGATTTTTAGAAAACTTCAGGAGGATAGGCGGTTAAAAGAGGAAGTTATGCGTGCCAAGATGGAAAAAACAGCTCGCTTGAAAGCTGAAACTAAGGAAAGAACACTGAAAAAGTTTCTGCTATCCCAGAAAGACGTGGTTTACACCGAGCCTCTAGAGATTCAAGCAGGAAACCCTGTGACTGTATTGTACAATCCTGCAAACACGGTTTTGAATGGAAAACCTGAAGTTTGGTTTAGAGGCTCTTTTAATCGTTGGACTCACCGCTTGGGCCCTTTGCCACCTCAGAAAATGGAAGCAACAGATGATGAAAGCTCACATGTGAAGACTACGGCTAAGGTCCCATTGGATGCTTACATGATGGACTTTGTGTTCTCTGAGAAAGAGGATGGCGGAATATTTGATAACAAAAATGGTCTGGATTACCATTTACCAGTCGTGGGAGGTATTTCAAAGGAACCACCATTGCACATTGTTCATATTGCTGTTGAAATGGCACCCATCGCAAAGGTTGGTGGCCTAGGTGATGTTGTCACTAGTCTATCTCGCGCTGTTCAAGAATTAAACCATAATGTGGATATAGTTTTTCCAAAGTATGATTGCATAAAGCACAATTTTGTGAAGGACTTGCAATTTAACAGAAGCTATCACTGGGGAGGAACTGAAATAAAAGTTTGGCATGGAAAAGTAGAAGGCCTTTCGGTTTACTTCTTAGATCCACAAAATGGATTGTTTCAGCGAGGATGTGTTTACGGTTGTGCAGATGATGCAGGAAGATTCGGTTTCTTCTGTCATGCGGCTCTTGAATTTCTTCTCCAAGGAGGTTTCCATCCAGACATTCTTCACTGTCATGACTGGTCTAGTGCTCCGGTTTCATGGTTATTCAAGGATCATTACACACAGTACGGTTTAATTAAAACCCGTATTGTCTTCACAATTCATAATTTGGAATTTGGAGCGAATGCCATTGGTAAAGCAATGACATTTGCAGACAAAGCCACAACGGTTTCACCAACTTATGCTAAGGAAGTTGCTGGAAACTCTGTAATCTCTGCACATTTATACAAATTTCACGGAATTATAAACGGGATTGACCCAGATATATGGGATCCATATAACGATAACTTTATTCCCGTACCTTATACTTCAGAGAACGTTGTAGAAGGCAAAAGAGCAGCCAAGGAAGAATTGCAAAACAGGCTTGGACTAAAGAGTGCCGATTTTCCAGTAGTAGGAATTATTACGCGCTTAACACACCAGAAGGGAATACATTTGATCAAGCACGCTATTTGGCGTACCTTGGAACGGAATGGACAGGTTGTCTTATTAGGTTCAGCTCCAGATCCTCGGATCCAAAATGATTTTGTAAACTTGGCAAACCAATTACATTCTTCTCATGGTGACCGGGCTCGGCTTGTTCTAACCTACGATGAACCTCTTTCCCATTTGATTTATGCTGGGGCTGACTTTATTCTTGTACCGTCGATATTTGAGCCATGTGGACTGACACAGCTCATAGCCATGAGATACGGCGCTGTTCCTGTTGTTAGAAAAACTGGAGGACTCTTTGATACGGTTTTTGATGTTGACCACGATAAAGAAAGGGCACAAGCTCAAGTTCTAGAACCTAATGGTTTCAGCTTCGACGGAGCTGATGCTCCTGGTGTTGATTATGCTCTCAATAGGGCGATATCGGCGTGGTACGATGGTAGAGAGTGGTTTAACTCGCTGTGCAAGACGGTGATGGAGCAAGACTGGTCATGGAACCGTCCTGCACTTGAGTATCTTGAGCTCTATCACTCTGCACGCAAGTAA

Protein Analysis

1042

Amino Acids

118.51

Weight (kDa)

6.09

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 157 - 216 1.8e-07 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 331 - 398 6.9e-12 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 498 - 561 2.2e-10 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 595 - 784 2.2e-51 Starch synthase catalytic domain
Glyco_transf_4 PF13439 609 - 805 2.3e-08 Glycosyltransferase Family 4
Glyco_trans_1_4 PF13692 847 - 964 6.2e-06 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2396
AccB1I GGYRCC 1 cut(s) 1810
AccB7I CCANNNNNTGG 2 cut(s) 1888, 2039
AccBSI CCGCTC 1 cut(s) 1137
AccI GTMKAC 1 cut(s) 494
AccII CGCG 2 cut(s) 1863, 2560
AciI CCGC 9 cut(s) 210, 401, 881, 1137, 1173, 1345, 1579, 1707, 2111
AclI AACGTT 1 cut(s) 2469
AcsI RAATTY 5 cut(s) 406, 742, 2120, 2264, 2381
AcuI CTGAAG 7 cut(s) 45, 1002, 1176, 1305, 1316, 1560, 2445
AfaI GTAC 8 cut(s) 228, 956, 1505, 2219, 2451, 2611, 2793, 3017
AfiI CCNNNNNNNGG 7 cut(s) 1136, 1217, 1754, 1888, 2039, 2546, 2619
AflIII ACRYGT 1 cut(s) 1633
AhdI GACNNNNNGTC 1 cut(s) 2633
AhlI ACTAGT 2 cut(s) 1180, 1850
AjiI CACGTC 2 cut(s) 899, 1450
AjnI CCWGG 3 cut(s) 581, 1168, 2974
AloI GAACNNNNNNTCC 2 cut(s) 1670, 1702
Alw21I GWGCWC 5 cut(s) 121, 871, 1180, 2182, 3108
Alw26I GTCTC 4 cut(s) 614, 878, 889, 940
Alw44I GTGCAC 1 cut(s) 1176
AoxI GGCC 3 cut(s) 1585, 1831, 2008
ApaI GGGCCC 1 cut(s) 1589
ApaLI GTGCAC 1 cut(s) 1176
ApeKI GCWGC 5 cut(s) 56, 212, 398, 848, 2489
ApoI RAATTY 5 cut(s) 406, 742, 2120, 2264, 2381
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
Asp700I GAANNNNTTC 4 cut(s) 474, 557, 906, 2124
AspA2I CCTAGG 1 cut(s) 1834
AspLEI GCGC 3 cut(s) 1865, 2562, 2849
AspS9I GGNCC 7 cut(s) 65, 80, 1019, 1208, 1585, 1586, 1654
AsuC2I CCSGG 1 cut(s) 2720
AsuHPI GGTGA 7 cut(s) 158, 431, 1568, 1850, 2316, 2726, 3067
AvaII GGWCC 5 cut(s) 65, 80, 1019, 1208, 1654
AvrII CCTAGG 1 cut(s) 1834
BaeGI GKGCMC 3 cut(s) 1180, 1589, 2923
BaeI ACNNNNGTAYC 4 cut(s) 3007, 3040, 3080, 3113
BamHI GGATCC 3 cut(s) 585, 2422, 2664
BanI GGYRCC 1 cut(s) 1810
BanII GRGCYC 3 cut(s) 1589, 2725, 3108
BarI GAAGNNNNNNTAC 4 cut(s) 151, 183, 986, 1018
BbsI GAAGAC 3 cut(s) 468, 1646, 2236
Bbv12I GWGCWC 5 cut(s) 121, 871, 1180, 2182, 3108
BbvI GCAGC 5 cut(s) 43, 224, 410, 835, 2501
BceAI ACGGC 2 cut(s) 1662, 2860
BciT130I CCWGG 3 cut(s) 583, 1170, 2976
BciVI GTATCC 1 cut(s) 447
BclI TGATCA 2 cut(s) 522, 2589
BcnI CCSGG 1 cut(s) 2720
BcoDI GTCTC 4 cut(s) 614, 878, 889, 940
BcuI ACTAGT 2 cut(s) 1180, 1850
BfaI CTAG 7 cut(s) 752, 1181, 1469, 1835, 1851, 2175, 2936
BfmI CTRYAG 2 cut(s) 171, 930
BfoI RGCGCY 1 cut(s) 2850
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 6 cut(s) 57, 213, 399, 849, 2112, 2490
BlnI CCTAGG 1 cut(s) 1834
BlsI GCNGC 6 cut(s) 58, 214, 400, 850, 2113, 2491
Bme1390I CCNGG 4 cut(s) 583, 1170, 2720, 2976
Bme18I GGWCC 5 cut(s) 65, 80, 1019, 1208, 1654
BmeRI GACNNNNNGTC 1 cut(s) 2633
BmgBI CACGTC 2 cut(s) 899, 1450
BmgT120I GGNCC 7 cut(s) 65, 80, 1019, 1208, 1585, 1586, 1654
BmiI GGNNCC 9 cut(s) 82, 587, 1587, 1656, 1773, 1812, 2424, 2666, 3080
BmrFI CCNGG 4 cut(s) 583, 1170, 2720, 2976
BmrI ACTGGG 2 cut(s) 1201, 1975
BmsI GCATC 3 cut(s) 1654, 2071, 2959
BmuI ACTGGG 2 cut(s) 1201, 1975
BpiI GAAGAC 3 cut(s) 468, 1646, 2236
BpmI CTGGAG 2 cut(s) 2637, 2892
Bpu10I CCTNAGC 3 cut(s) 76, 1650, 2337
BpuEI CTTGAG 3 cut(s) 2913, 3113, 3122
BpuMI CCSGG 1 cut(s) 2720
BsaBI GATNNNNATC 1 cut(s) 1125
BsaJI CCNNGG 6 cut(s) 786, 1834, 2131, 2493, 2613, 2660
BsaWI WCCGGW 1 cut(s) 2182
Bsc4I CCNNNNNNNGG 7 cut(s) 1136, 1217, 1754, 1888, 2039, 2546, 2619
Bse118I RCCGGY 1 cut(s) 913
Bse1I ACTGG 8 cut(s) 1196, 1301, 1748, 1970, 2174, 2540, 2875, 3074
Bse3DI GCAATG 2 cut(s) 1778, 2298
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 3 cut(s) 583, 1170, 2976
BseDI CCNNGG 6 cut(s) 786, 1834, 2131, 2493, 2613, 2660
BseGI GGATG 8 cut(s) 229, 295, 726, 1126, 1669, 1708, 2063, 2143
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 7 cut(s) 1136, 1217, 1754, 1888, 2039, 2546, 2619
BseMI GCAATG 2 cut(s) 1778, 2298
BseMII CTCAG 9 cut(s) 90, 134, 146, 789, 902, 933, 1101, 1613, 1683
BseNI ACTGG 8 cut(s) 1196, 1301, 1748, 1970, 2174, 2540, 2875, 3074
BseSI GKGCMC 3 cut(s) 1180, 1589, 2923
BseXI GCAGC 5 cut(s) 43, 224, 410, 835, 2501
BseYI CCCAGC 1 cut(s) 2771
BsgI GTGCAG 4 cut(s) 2094, 2352, 3072, 3102
Bsh1236I CGCG 2 cut(s) 1863, 2560
BshFI GGCC 3 cut(s) 1587, 1833, 2010
BshNI GGYRCC 1 cut(s) 1810
BsiHKAI GWGCWC 5 cut(s) 121, 871, 1180, 2182, 3108
BsiSI CCGG 3 cut(s) 914, 2183, 2719
BslFI GGGAC 2 cut(s) 66, 1640
BslI CCNNNNNNNGG 7 cut(s) 1136, 1217, 1754, 1888, 2039, 2546, 2619
BsmAI GTCTC 4 cut(s) 614, 878, 889, 940
BsmBI CGTCTC 1 cut(s) 889
BsmFI GGGAC 2 cut(s) 66, 1640
BsmI GAATGC 1 cut(s) 2281
BsnI GGCC 3 cut(s) 1587, 1833, 2010
Bsp120I GGGCCC 1 cut(s) 1585
Bsp1286I GDGCHC 8 cut(s) 121, 871, 1180, 1589, 2182, 2725, 2923, 3108
Bsp1407I TGTACA 2 cut(s) 226, 1503
BspACI CCGC 9 cut(s) 210, 401, 881, 1137, 1173, 1345, 1579, 1707, 2111
BspANI GGCC 3 cut(s) 1587, 1833, 2010
BspCNI CTCAG 9 cut(s) 89, 133, 145, 790, 901, 934, 1102, 1612, 1684
BspFNI CGCG 2 cut(s) 1863, 2560
BspHI TCATGA 1 cut(s) 2164
BspLI GGNNCC 9 cut(s) 82, 587, 1587, 1656, 1773, 1812, 2424, 2666, 3080
BspT107I GGYRCC 1 cut(s) 1810
BsrBI CCGCTC 1 cut(s) 1137
BsrDI GCAATG 2 cut(s) 1778, 2298
BsrFI RCCGGY 1 cut(s) 913
BsrGI TGTACA 2 cut(s) 226, 1503
BsrI ACTGG 8 cut(s) 1196, 1301, 1748, 1970, 2174, 2540, 2875, 3074
BssAI RCCGGY 1 cut(s) 913
BssECI CCNNGG 6 cut(s) 786, 1834, 2131, 2493, 2613, 2660
BssT1I CCWWGG 5 cut(s) 786, 1834, 2131, 2493, 2613
Bst2UI CCWGG 3 cut(s) 583, 1170, 2976
Bst6I CTCTTC 4 cut(s) 750, 813, 818, 887
BstAPI GCANNNNNTGC 1 cut(s) 2081
BstAUI TGTACA 2 cut(s) 226, 1503
BstC8I GCNNGC 6 cut(s) 210, 396, 1368, 1390, 2598, 3121
BstEII GGTNACC 2 cut(s) 1244, 2714
BstF5I GGATG 8 cut(s) 229, 295, 726, 1126, 1669, 1708, 2063, 2143
BstFNI CGCG 2 cut(s) 1863, 2560
BstH2I RGCGCY 1 cut(s) 2850
BstHHI GCGC 3 cut(s) 1865, 2562, 2849
BstMAI GTCTC 4 cut(s) 614, 878, 889, 940
BstMWI GCNNNNNNNGC 8 cut(s) 845, 857, 866, 1817, 2081, 2309, 2512, 3008
BstNI CCWGG 3 cut(s) 583, 1170, 2976
BstNSI RCATGY 1 cut(s) 1637
BstPI GGTNACC 2 cut(s) 1244, 2714
BstSCI CCNGG 4 cut(s) 581, 1168, 2718, 2974
BstSFI CTRYAG 2 cut(s) 171, 930
BstSLI GKGCMC 3 cut(s) 1180, 1589, 2923
BstUI CGCG 2 cut(s) 1863, 2560
BstV1I GCAGC 5 cut(s) 43, 224, 410, 835, 2501
BstV2I GAAGAC 3 cut(s) 468, 1646, 2236
BstX2I RGATCY 5 cut(s) 585, 2029, 2422, 2656, 2664
BstXI CCANNNNNNTGG 1 cut(s) 2418
BstYI RGATCY 5 cut(s) 585, 2029, 2422, 2656, 2664
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 3 cut(s) 1587, 1833, 2010
BtgZI GCGATG 1 cut(s) 1801
BtrI CACGTC 2 cut(s) 899, 1450
BtsCI GGATG 8 cut(s) 229, 295, 726, 1126, 1669, 1708, 2063, 2143
BtsIMutI CAGTG 4 cut(s) 996, 1418, 1963, 2158
Cac8I GCNNGC 6 cut(s) 210, 396, 1368, 1390, 2598, 3121
CciI TCATGA 1 cut(s) 2164
CfoI GCGC 3 cut(s) 1865, 2562, 2849
Cfr10I RCCGGY 1 cut(s) 913
Cfr13I GGNCC 7 cut(s) 65, 80, 1019, 1208, 1585, 1586, 1654
Csp6I GTAC 8 cut(s) 227, 955, 1504, 2218, 2450, 2610, 2792, 3016
CviQI GTAC 8 cut(s) 227, 955, 1504, 2218, 2450, 2610, 2792, 3016
DriI GACNNNNNGTC 1 cut(s) 2633
Eam1104I CTCTTC 4 cut(s) 750, 813, 818, 887
Eam1105I GACNNNNNGTC 1 cut(s) 2633
EarI CTCTTC 4 cut(s) 750, 813, 818, 887
EciI GGCGGA 2 cut(s) 896, 1722
Ecl136II GAGCTC 1 cut(s) 3106
Eco130I CCWWGG 5 cut(s) 786, 1834, 2131, 2493, 2613
Eco147I AGGCCT 1 cut(s) 2010
Eco24I GRGCYC 3 cut(s) 1589, 2725, 3108
Eco32I GATATC 1 cut(s) 3006
Eco47I GGWCC 5 cut(s) 65, 80, 1019, 1208, 1654
Eco53kI GAGCTC 1 cut(s) 3106
Eco57I CTGAAG 7 cut(s) 45, 1002, 1176, 1305, 1316, 1560, 2445
Eco91I GGTNACC 2 cut(s) 1244, 2714
EcoICRI GAGCTC 1 cut(s) 3106
EcoO109I RGGNCCY 4 cut(s) 80, 1019, 1586, 1654
EcoO65I GGTNACC 2 cut(s) 1244, 2714
EcoRII CCWGG 3 cut(s) 581, 1168, 2974
EcoRV GATATC 1 cut(s) 3006
EcoT14I CCWWGG 5 cut(s) 786, 1834, 2131, 2493, 2613
EcoT38I GRGCYC 3 cut(s) 1589, 2725, 3108
ErhI CCWWGG 5 cut(s) 786, 1834, 2131, 2493, 2613
Esp3I CGTCTC 1 cut(s) 889
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 2 cut(s) 66, 1640
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 2 cut(s) 522, 2589
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 6 cut(s) 57, 213, 399, 849, 2112, 2490
FokI GGATG 8 cut(s) 236, 302, 733, 1133, 1676, 1715, 2070, 2130
FriOI GRGCYC 3 cut(s) 1589, 2725, 3108
Fsp4HI GCNGC 6 cut(s) 57, 213, 399, 849, 2112, 2490
FspBI CTAG 7 cut(s) 752, 1181, 1469, 1835, 1851, 2175, 2936
GlaI GCGC 3 cut(s) 1864, 2561, 2848
GluI GCNGC 6 cut(s) 57, 213, 399, 849, 2112, 2490
GsaI CCCAGC 1 cut(s) 2775
GsuI CTGGAG 2 cut(s) 2637, 2892
HaeII RGCGCY 1 cut(s) 2850
HaeIII GGCC 3 cut(s) 1587, 1833, 2010
HapII CCGG 3 cut(s) 914, 2183, 2719
HhaI GCGC 3 cut(s) 1865, 2562, 2849
Hin6I GCGC 3 cut(s) 1863, 2560, 2847
HinP1I GCGC 3 cut(s) 1863, 2560, 2847
HincII GTYRAC 3 cut(s) 495, 733, 2902
HindII GTYRAC 3 cut(s) 495, 733, 2902
HindIII AAGCTT 1 cut(s) 1097
HpaII CCGG 3 cut(s) 914, 2183, 2719
HphI GGTGA 7 cut(s) 158, 431, 1568, 1850, 2316, 2726, 3067
Hpy166II GTNNAC 9 cut(s) 495, 733, 1178, 1456, 2020, 2065, 2683, 2816, 2902
Hpy8I GTNNAC 9 cut(s) 495, 733, 1178, 1456, 2020, 2065, 2683, 2816, 2902
Hpy99I CGWCG 3 cut(s) 102, 2800, 2963
HpyAV CCTTC 6 cut(s) 373, 623, 1930, 2000, 2471, 2569
HpyCH4IV ACGT 3 cut(s) 898, 1449, 2469
HpyF10VI GCNNNNNNNGC 8 cut(s) 845, 857, 866, 1817, 2081, 2309, 2512, 3008
HpySE526I ACGT 3 cut(s) 898, 1449, 2469
HspAI GCGC 3 cut(s) 1863, 2560, 2847
Ksp22I TGATCA 2 cut(s) 522, 2589
LmnI GCTCC 7 cut(s) 1220, 2185, 2270, 2656, 2963, 2977, 3061
Lsp1109I GCAGC 5 cut(s) 43, 224, 410, 835, 2501
LweI GCATC 3 cut(s) 1654, 2071, 2959
MaeI CTAG 7 cut(s) 752, 1181, 1469, 1835, 1851, 2175, 2936
MaeII ACGT 3 cut(s) 898, 1449, 2469
MaeIII GTNAC 5 cut(s) 1047, 1244, 1492, 1846, 2714
MbiI CCGCTC 1 cut(s) 1137
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 5 cut(s) 585, 2029, 2422, 2656, 2664
MhlI GDGCHC 8 cut(s) 121, 871, 1180, 1589, 2182, 2725, 2923, 3108
MlyI GAGTC 5 cut(s) 490, 680, 880, 1567, 2871
MmeI TCCRAC 2 cut(s) 258, 1550
MroXI GAANNNNTTC 4 cut(s) 474, 557, 906, 2124
MslI CAYNNNNRTG 3 cut(s) 470, 2163, 2834
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 3 cut(s) 914, 2183, 2719
MspR9I CCNGG 4 cut(s) 583, 1170, 2720, 2976
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 2281
MvaI CCWGG 3 cut(s) 583, 1170, 2976
MvnI CGCG 2 cut(s) 1863, 2560
MwoI GCNNNNNNNGC 8 cut(s) 845, 857, 866, 1817, 2081, 2309, 2512, 3008
NciI CCSGG 1 cut(s) 2720
NlaIV GGNNCC 9 cut(s) 82, 587, 1587, 1656, 1773, 1812, 2424, 2666, 3080
NmeAIII GCCGAG 3 cut(s) 917, 990, 2704
NmuCI GTSAC 3 cut(s) 1492, 1846, 2714
NspI RCATGY 1 cut(s) 1637
PacI TTAATTAA 1 cut(s) 2229
PagI TCATGA 1 cut(s) 2164
PceI AGGCCT 1 cut(s) 2010
PciI ACATGT 1 cut(s) 1633
PctI GAATGC 1 cut(s) 2281
PdmI GAANNNNTTC 4 cut(s) 474, 557, 906, 2124
PfeI GAWTC 5 cut(s) 367, 487, 831, 1474, 2091
PflFI GACNNNGTC 1 cut(s) 1193
PflMI CCANNNNNTGG 2 cut(s) 1888, 2039
PkrI GCNGC 6 cut(s) 58, 214, 400, 850, 2113, 2491
PleI GAGTC 5 cut(s) 490, 680, 880, 1567, 2871
PpsI GAGTC 5 cut(s) 490, 680, 880, 1567, 2871
PpuMI RGGWCCY 3 cut(s) 80, 1019, 1654
PscI ACATGT 1 cut(s) 1633
PsiI TTATAA 1 cut(s) 2396
Psp124BI GAGCTC 1 cut(s) 3108
Psp1406I AACGTT 1 cut(s) 2469
Psp5II RGGWCCY 3 cut(s) 80, 1019, 1654
Psp6I CCWGG 3 cut(s) 581, 1168, 2974
PspEI GGTNACC 2 cut(s) 1244, 2714
PspFI CCCAGC 1 cut(s) 2771
PspGI CCWGG 3 cut(s) 581, 1168, 2974
PspN4I GGNNCC 9 cut(s) 82, 587, 1587, 1656, 1773, 1812, 2424, 2666, 3080
PspOMI GGGCCC 1 cut(s) 1585
PspPI GGNCC 7 cut(s) 65, 80, 1019, 1208, 1585, 1586, 1654
PspPPI RGGWCCY 3 cut(s) 80, 1019, 1654
PsrI GAACNNNNNNTAC 2 cut(s) 1032, 1064
PsuI RGATCY 5 cut(s) 585, 2029, 2422, 2656, 2664
PsyI GACNNNGTC 1 cut(s) 1193
RsaI GTAC 8 cut(s) 228, 956, 1505, 2219, 2451, 2611, 2793, 3017
RsaNI GTAC 8 cut(s) 227, 955, 1504, 2218, 2450, 2610, 2792, 3016
RseI CAYNNNNRTG 3 cut(s) 470, 2163, 2834
SacI GAGCTC 1 cut(s) 3108
SalI GTCGAC 1 cut(s) 493
SatI GCNGC 6 cut(s) 57, 213, 399, 849, 2112, 2490
Sau96I GGNCC 7 cut(s) 65, 80, 1019, 1208, 1585, 1586, 1654
SchI GAGTC 5 cut(s) 490, 680, 880, 1567, 2871
ScrFI CCNGG 4 cut(s) 583, 1170, 2720, 2976
SduI GDGCHC 8 cut(s) 121, 871, 1180, 1589, 2182, 2725, 2923, 3108
SfaNI GCATC 3 cut(s) 1654, 2071, 2959
SfcI CTRYAG 2 cut(s) 171, 930
SinI GGWCC 5 cut(s) 65, 80, 1019, 1208, 1654
SmiMI CAYNNNNRTG 3 cut(s) 470, 2163, 2834
SmlI CTYRAG 3 cut(s) 2928, 3092, 3101
SmoI CTYRAG 3 cut(s) 2928, 3092, 3101
SpeI ACTAGT 2 cut(s) 1180, 1850
SseBI AGGCCT 1 cut(s) 2010
SsiI CCGC 9 cut(s) 210, 401, 881, 1137, 1173, 1345, 1579, 1707, 2111
SspI AATATT 1 cut(s) 1713
SspMI CTAG 7 cut(s) 752, 1181, 1469, 1835, 1851, 2175, 2936
SstI GAGCTC 1 cut(s) 3108
StuI AGGCCT 1 cut(s) 2010
StyD4I CCNGG 4 cut(s) 581, 1168, 2718, 2974
StyI CCWWGG 5 cut(s) 786, 1834, 2131, 2493, 2613
TaiI ACGT 3 cut(s) 901, 1452, 2472
TaqI TCGA 4 cut(s) 494, 834, 2798, 2958
TatI WGTACW 2 cut(s) 226, 1503
TauI GCSGC 1 cut(s) 2114
TfiI GAWTC 5 cut(s) 367, 487, 831, 1474, 2091
TscAI CASTG 4 cut(s) 996, 1425, 1970, 2165
TseFI GTSAC 3 cut(s) 1492, 1846, 2714
TseI GCWGC 5 cut(s) 56, 212, 398, 848, 2489
Tsp45I GTSAC 3 cut(s) 1492, 1846, 2714
TspDTI ATGAA 8 cut(s) 474, 605, 606, 1638, 1781, 2178, 2243, 2760
TspGWI ACGGA 3 cut(s) 2403, 2635, 2976
TspRI CASTG 4 cut(s) 996, 1425, 1970, 2165
Tth111I GACNNNGTC 1 cut(s) 1193
Van91I CCANNNNNTGG 2 cut(s) 1888, 2039
VneI GTGCAC 1 cut(s) 1176
VpaK11BI GGWCC 5 cut(s) 65, 80, 1019, 1208, 1654
XapI RAATTY 5 cut(s) 406, 742, 2120, 2264, 2381
XbaI TCTAGA 3 cut(s) 751, 1468, 2935
XceI RCATGY 1 cut(s) 1637
XmaJI CCTAGG 1 cut(s) 1834
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 4 cut(s) 474, 557, 906, 2124
XspI CTAG 7 cut(s) 752, 1181, 1469, 1835, 1851, 2175, 2936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.