Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0003232

Physical Location & Seq

Genomic Coordinates Forward (+)

31236752 .. 31243597

Gene Structure 6846 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g31510.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3312 bp

ATGGAGGTTTCTATACATGCACAGAGGCCGCTCAGATGCAGAGGACTTTTCCAACCGGGAAGAAGCATCTTAAAGCTCCAACCTTTTACTGGGTTCTCTCCCCATGGAAGGTATTCTTCATGGTTCAAAGGAAACCTGACAAGTGGGGTTTCATACCAAATTACTGCCAGTTCGGCCGATTTTTCAAGGAGGAGACAAAGAAAAGTGTCTCCTGGTTCAAAGGGATTAGTGCCAGAAACCCCAGCAGGAACTAGCGTCCAGAAGACGAATCAAAGGAATATTGGGGACGAAAAGGGTATTACATCTTCGACATCCAGTGAGCTTGCTGGGAAAAATAAGAAAACACATCAGTTGAGGGTTGACACCAGAAGAGAATGGGCACTTGAACCTTCAGAAGAGAGCAATGTTGACGAAAAGAGAATTGATGAAACTAGTAGTAAAGTCGAGGAATCATCTTCAGTTGGCAAGCAGACAGCAATTGATAGGGGTATTCGAGGGATAGAAAATGGGAGTGTAGGTAAGGTCCTTGATGACATCTCAGATACTGATAAAAAGTTAACAAATGAAGACCCCTTAAAGTTAAAGTTGGAGAGGGAAGAAAAATTGCGTAAAGAGGAAATTGCGAGGCTTGCTGAGGAAAACTTCTCAAGAGGGAACAAAATATTTTTCTATCCTCAGGCCGTGAAACCTGATCAAGACATAGAAGTGTTCCTCAATAGAAGTATATCAACCCTAAGCAATGAGCCGGATGTTCAAATAATGGGAGCCTTTAACGACTGGCGTTGGAAATCTTTTACCTTCAGGTTGAACAAAACACAACTCAAGGGGGATTGGTGGTCTTGCCAGTTTCATGTTCCTAAAGAGTCATATAAAATAGACTTTGTGTTCTTTAATGGGCAAAATGTTTATGACAATAATGATGCAAAAGATTTCTGCATAACTGTGGAAGGTGGAATGGACCTCTTTGCATTTGAAGATTTCTTGCTTGAGGAGAAGCGTAAAGAACAAGAGAAACTTGCGAAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATTGAAGCGGAGAAGGCAGCAAGTGAAGCTGACAGAGCACAGGCAAAGGCAGAGATCGTAAAGAGACGACAAATGGTTCAAGAGTTGATAAAAAAGGCTGTAAGGTCTGTAGAAGACGTTTGGCACATAGAGCCTAAAGAGTTTAAAGGCGAGGACTTGGTGAAGTTATACTATAACCGAAGCTTAGGTCCTCTTGCTAATGTTAAGGAACTATGGATTCATGGGGGGCATAATGGTTGGAAGGATGGATTGTCAATTGTTGAAAGGCTCGTTAGCTCTGAGAGAAAGGATGGTGACTGGTGGTATGCCAATGTTGTTGTACCTGAACGAGCCGTTGTCTTGGATTGGGTTTTTGCTGATGGACCACCCCAAAATGCCGTTTTATATGATAACAACCACCGTCATGATTTCCATGCTATAGTTCCAAAATCCATTCCTGACGAACTATATTGGGTTGAGGAAGAACACCAGATATATAAGCAGCTCCAGGAGGAGAGGAGATTAAGGGAGGAGGCAATACGTGCCAAGGCCGAAAGAACAGCTCGTATGAAAGCAGAAATGAAGGAAAGAACTTTGAAAAGGTTTTTGCTGTCTCAGAAGCATATAGTATACACTGAGCCTCTTGATGTGCAAGCTGGTAACAAGGTGACAGTTTTCTACAATCCTGCCAACACAGTTCTGAATGGAAAGCCTGAGGTATGGTTCAGAGGATCATTTAACCGTTGGAACCATCGTAAGGGTCCATTGCAACCACAGAAGATGTTACCTTCGGAGAATGGTTCTCATGTCAAAACCACTGTTTCAGTTCCGTTGGATGCATACATGATGGACTTTGTTTTCTCTGAGACGGAAGATGGTGGACTTTTTGACAACAAAAATGGCATGGATTACCACATTCCAGTGTTTGGAGGAGTGGTTAAGGAGTCACCAATGAACATTGTTGGAGGTCTTGGTGATGTGGTTACGAGTCTCTCACGAGCTGTGCAAGACTTAAATCACCACGTCGACATCATTCTTCCAAAGTATGACTGTTTGAACCTTAGCAATGTGAAGGAGTTTCAGTATAACAGAGGCTTCTTTTGGGGAGGAACTGAAATAAAAGTTTGGTTTGGGAAAGTGGAAGGTGTTTCGGTCTACTTTTTGGAGCCTCAAAACAGATTCTTTCATGCAGGTTGCATATATGGTTGTAAAAATGATGCAGAGAGATTTGGTTTCTTTTGCCATGCTGCGCTAGAATTTCTACTCCAAAGTGGATTTCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCACCTGTTGCATGGTTATTTAAAGATCATTACATGCATTATGGTCTTAGTAAAGCTCGGATTGTTTTCACAATTCATAACCTTGAGTTTGGAGCGCCCTTCATTGGTAAAGCTGTGGCATATTCAGACAAGTCCACGACTGTATCCAACACTTATGCTAAGGAGGTATCTGGAAATCCTGCAGTTGCTCCGCATCTTTACAAGTTTCATGGTATAATAAATGGAATTGACCAAGATATATGGGATCCATACAATGATAAGTTCATTCCTCTATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGCTGATCTCCCTGTAGTAGGTATTATCACTCGCTTAACTCACCAGAAAGGAATCCATCTCATCAAGCATGCCATTTGGCGTACCTTGGAACGCAATGGGCAGGTCGTATTGCTTGGGTCAGCTCCAGATCCTCGTATCCAAAATGATTTTGTAAATTTGGCAAATCAGTTACATTCTTCTCATGGTGATCGTGCTCGCCTCTGTTTAACTTATGATGAGCCACTGTCGCATTTGATATACGCTGGTGCTGATTTCATCTTAGTTCCTTCAATTTTTGAGCCATGCGGTCTCACCCAACTCATAGCAATGAGATATGGTTCAATACCTGTTGTTCGAAAAACTGGAGGACTTTATGATACGGTATTTGATGTTGACCATGATAAAGAGAGAGCAGACGTCCAGGGTGTCGAGCCAAATGGTTTCAGCTTTGATGGAGCTGATGCTGCTGGTGTCGATTATGCTCTTAATAGGGCAATCTCCGCTTGGTATGATGGTCGGGATTGGTTTAACTCGCTGTGCAAGACGGTGATGCAGCAAGATTGGTCTTGGAACAAGCCAGCTCTCGACTATATGGAGCTTTACCACGCAGCACGGAAGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0010021 GO:0042802 GO:0043170 GO:0071704 GO:1901135 GO:1901137 GO:1901576 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

CBM53 Glyco_transf_5 Glycos_transf_1

Protein Analysis

1104

Amino Acids

125.86

Weight (kDa)

6.55

Isoelectric Point (pI)

43.17

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11720 AT1G11720
fragaria_vesca	FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040
malus_domestica	MD05G1347400.v1.1 MD10G1321600.v1.1
prunus_persica	Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1
pyrus_communis	pycom05g31510 pycom10g27230
rosa_chinensis	RchiOBHm_Chr5g0002941
rosa_laevigata	RLG00000031072
rosa_multiflora	Rmu_sc0013603.1_g000004
rosa_roxburghii	Rroxscaffold_1G00072920
rosa_rugosa	Rorug04G0400700 Rorug04G0400800 Rorug04G0400800
rosa_samantha	Rh5AG023700 Rh5BG026500 Rh5CG026400 Rh5DG025000
rosa_wichuraiana	Rw5G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3108
Acc36I	ACCTGC	2 cut(s)	2225, 2800
AccB7I	CCANNNNNTGG	1 cut(s)	1970
AccBSI	CCGCTC	1 cut(s)	31
AccI	GTMKAC	3 cut(s)	1674, 2070, 2199
AciI	CCGC	5 cut(s)	29, 1073, 2549, 2994, 3189
AclWI	GGATC	4 cut(s)	1783, 2597, 2610, 2831
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	2 cut(s)	2300, 2863
AcuI	CTGAAG	3 cut(s)	375, 441, 784
AcyI	GRCGYC	1 cut(s)	3105
AfaI	GTAC	2 cut(s)	1386, 2791
AfiI	CCNNNNNNNGG	6 cut(s)	89, 108, 1801, 1970, 2462, 2726
AhlI	ACTAGT	1 cut(s)	431
AjiI	CACGTC	1 cut(s)	2068
AjnI	CCWGG	3 cut(s)	211, 1551, 3108
AjuI	GAANNNNNNNTTGG	2 cut(s)	2304, 2336
Alw21I	GWGCWC	3 cut(s)	1105, 2362, 2905
Alw26I	GTCTC	7 cut(s)	187, 213, 1123, 1662, 1904, 2039, 3002
Alw44I	GTGCAC	1 cut(s)	2358
AlwI	GGATC	4 cut(s)	1783, 2597, 2610, 2831
AlwNI	CAGNNNCTG	1 cut(s)	545
AoxI	GGCC	4 cut(s)	26, 174, 678, 1593
ApaLI	GTGCAC	1 cut(s)	2358
ApeKI	GCWGC	7 cut(s)	1082, 1546, 2291, 2669, 3152, 3241, 3296
ApoI	RAATTY	2 cut(s)	2300, 2863
ArsI	GACNNNNNNTTYG	4 cut(s)	1237, 1269, 2683, 2715
Asp700I	GAANNNNTTC	1 cut(s)	112
AspLEI	GCGC	2 cut(s)	2296, 2455
AspS9I	GGNCC	5 cut(s)	523, 958, 1253, 1427, 1805
AsuC2I	CCSGG	1 cut(s)	57
AsuII	TTCGAA	1 cut(s)	3043
AvaII	GGWCC	5 cut(s)	523, 958, 1253, 1427, 1805
AxyI	CCTNAGG	2 cut(s)	675, 1758
BaeGI	GKGCMC	2 cut(s)	382, 2362
BaeI	ACNNNNGTAYC	2 cut(s)	2484, 2517
BamHI	GGATCC	1 cut(s)	2602
BarI	GAAGNNNNNNTAC	2 cut(s)	289, 321
BauI	CACGAG	1 cut(s)	2040
BbsI	GAAGAC	3 cut(s)	269, 573, 1185
Bbv12I	GWGCWC	3 cut(s)	1105, 2362, 2905
BbvCI	CCTCAGC	1 cut(s)	633
BbvI	GCAGC	7 cut(s)	1094, 1558, 2278, 2656, 3139, 3253, 3308
BccI	CCATC	9 cut(s)	1304, 1349, 1418, 1803, 1885, 1913, 2772, 3134, 3194
BceAI	ACGGC	3 cut(s)	665, 1382, 1427
BciT130I	CCWGG	3 cut(s)	213, 1553, 3110
BciVI	GTATCC	2 cut(s)	2512, 2855
BclI	TGATCA	1 cut(s)	691
BcnI	CCSGG	1 cut(s)	57
BcoDI	GTCTC	7 cut(s)	187, 213, 1123, 1662, 1904, 2039, 3002
BcuI	ACTAGT	1 cut(s)	431
BfaI	CTAG	4 cut(s)	252, 432, 2297, 2355
BfmI	CTRYAG	4 cut(s)	1173, 1482, 2538, 2721
BfoI	RGCGCY	1 cut(s)	2456
BfuAI	ACCTGC	2 cut(s)	2225, 2800
BfuI	GTATCC	2 cut(s)	2512, 2855
BglI	GCCNNNNNGGC	1 cut(s)	173
BisI	GCNGC	8 cut(s)	29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
BlsI	GCNGC	8 cut(s)	30, 1084, 1548, 2293, 2671, 3154, 3243, 3298
Bme1390I	CCNGG	4 cut(s)	57, 213, 1553, 3110
Bme18I	GGWCC	5 cut(s)	523, 958, 1253, 1427, 1805
BmgBI	CACGTC	1 cut(s)	2068
BmgT120I	GGNCC	5 cut(s)	523, 958, 1253, 1427, 1805
BmiI	GGNNCC	5 cut(s)	766, 1793, 1806, 2211, 2604
BmrFI	CCNGG	4 cut(s)	57, 213, 1553, 3110
BmrI	ACTGGG	1 cut(s)	99
BmsI	GCATC	8 cut(s)	26, 75, 910, 1870, 2251, 2560, 3139, 3228
BmuI	ACTGGG	1 cut(s)	99
BpiI	GAAGAC	3 cut(s)	269, 573, 1185
BpmI	CTGGAG	3 cut(s)	1535, 2817, 3072
Bpu10I	CCTNAGC	5 cut(s)	633, 734, 1249, 2105, 2517
Bpu14I	TTCGAA	1 cut(s)	3043
BpuEI	CTTGAG	4 cut(s)	631, 806, 1007, 2462
BpuMI	CCSGG	1 cut(s)	57
BsaAI	YACGTR	1 cut(s)	1586
BsaHI	GRCGYC	1 cut(s)	3105
BsaI	GGTCTC	1 cut(s)	3002
BsaJI	CCNNGG	5 cut(s)	103, 1590, 2673, 2793, 3109
BsaXI	ACNNNNNCTCC	6 cut(s)	502, 532, 1965, 1995, 2111, 2141
Bsc4I	CCNNNNNNNGG	6 cut(s)	89, 108, 1801, 1970, 2462, 2726
Bse1I	ACTGG	8 cut(s)	94, 168, 315, 782, 844, 1367, 1964, 3055
Bse21I	CCTNAGG	2 cut(s)	675, 1758
Bse3DI	GCAATG	7 cut(s)	409, 745, 1808, 2116, 2338, 2809, 3021
BseBI	CCWGG	3 cut(s)	213, 1553, 3110
BseDI	CCNNGG	5 cut(s)	103, 1590, 2673, 2793, 3109
BseGI	GGATG	6 cut(s)	311, 754, 1315, 1360, 1885, 2323
BseLI	CCNNNNNNNGG	6 cut(s)	89, 108, 1801, 1970, 2462, 2726
BseMI	GCAATG	7 cut(s)	409, 745, 1808, 2116, 2338, 2809, 3021
BseNI	ACTGG	8 cut(s)	94, 168, 315, 782, 844, 1367, 1964, 3055
BseRI	GAGGAG	6 cut(s)	205, 1004, 1571, 1576, 1589, 1989
BseSI	GKGCMC	2 cut(s)	382, 2362
BseX3I	CGGCCG	1 cut(s)	174
BseXI	GCAGC	7 cut(s)	1094, 1558, 2278, 2656, 3139, 3253, 3308
BseYI	CCCAGC	2 cut(s)	241, 326
Bsh1285I	CGRYCG	1 cut(s)	177
BshFI	GGCC	4 cut(s)	28, 176, 680, 1595
BsiEI	CGRYCG	1 cut(s)	177
BsiHKAI	GWGCWC	3 cut(s)	1105, 2362, 2905
BsiSI	CCGG	2 cut(s)	56, 746
BslFI	GGGAC	1 cut(s)	299
BslI	CCNNNNNNNGG	6 cut(s)	89, 108, 1801, 1970, 2462, 2726
BsmAI	GTCTC	7 cut(s)	187, 213, 1123, 1662, 1904, 2039, 3002
BsmBI	CGTCTC	2 cut(s)	1123, 1904
BsmFI	GGGAC	1 cut(s)	299
BsnI	GGCC	4 cut(s)	28, 176, 680, 1595
Bso31I	GGTCTC	1 cut(s)	3002
Bsp119I	TTCGAA	1 cut(s)	3043
Bsp1286I	GDGCHC	4 cut(s)	382, 1105, 2362, 2905
Bsp143I	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
Bsp19I	CCATGG	1 cut(s)	103
BspACI	CCGC	5 cut(s)	29, 1073, 2549, 2994, 3189
BspANI	GGCC	4 cut(s)	28, 176, 680, 1595
BspHI	TCATGA	1 cut(s)	1468
BspLI	GGNNCC	5 cut(s)	766, 1793, 1806, 2211, 2604
BspMAI	CTGCAG	1 cut(s)	2542
BspMI	ACCTGC	2 cut(s)	2225, 2800
BspPI	GGATC	4 cut(s)	1783, 2597, 2610, 2831
BspQI	GCTCTTC	1 cut(s)	1047
BspT104I	TTCGAA	1 cut(s)	3043
BspTNI	GGTCTC	1 cut(s)	3002
BsrBI	CCGCTC	1 cut(s)	31
BsrDI	GCAATG	7 cut(s)	409, 745, 1808, 2116, 2338, 2809, 3021
BsrI	ACTGG	8 cut(s)	94, 168, 315, 782, 844, 1367, 1964, 3055
BssECI	CCNNGG	5 cut(s)	103, 1590, 2673, 2793, 3109
BssMI	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
BssNAI	GTATAC	1 cut(s)	1675
BssNI	GRCGYC	1 cut(s)	3105
BssSI	CACGAG	1 cut(s)	2040
BssT1I	CCWWGG	4 cut(s)	103, 1590, 2673, 2793
Bst1107I	GTATAC	1 cut(s)	1675
Bst2BI	CACGAG	1 cut(s)	2040
Bst2UI	CCWGG	3 cut(s)	213, 1553, 3110
Bst6I	CTCTTC	3 cut(s)	364, 390, 1047
BstACI	GRCGYC	1 cut(s)	3105
BstAPI	GCANNNNNTGC	2 cut(s)	1586, 2366
BstBAI	YACGTR	1 cut(s)	1586
BstBI	TTCGAA	1 cut(s)	3043
BstC8I	GCNNGC	9 cut(s)	324, 467, 630, 1029, 1047, 1698, 2778, 2905, 3267
BstDSI	CCRYGG	1 cut(s)	103
BstENI	CCTNNNNNAGG	1 cut(s)	2724
BstF5I	GGATG	6 cut(s)	311, 754, 1315, 1360, 1885, 2323
BstH2I	RGCGCY	1 cut(s)	2456
BstHHI	GCGC	2 cut(s)	2296, 2455
BstKTI	GATC	8 cut(s)	694, 1122, 1778, 2386, 2605, 2716, 2839, 2899
BstMAI	GTCTC	7 cut(s)	187, 213, 1123, 1662, 1904, 2039, 3002
BstMBI	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
BstMCI	CGRYCG	1 cut(s)	177
BstNI	CCWGG	3 cut(s)	213, 1553, 3110
BstNSI	RCATGY	3 cut(s)	20, 2395, 2780
BstSCI	CCNGG	4 cut(s)	55, 211, 1551, 3108
BstSFI	CTRYAG	4 cut(s)	1173, 1482, 2538, 2721
BstSLI	GKGCMC	2 cut(s)	382, 2362
BstV1I	GCAGC	7 cut(s)	1094, 1558, 2278, 2656, 3139, 3253, 3308
BstV2I	GAAGAC	3 cut(s)	269, 573, 1185
BstX2I	RGATCY	2 cut(s)	2602, 2836
BstYI	RGATCY	2 cut(s)	2602, 2836
BstZ17I	GTATAC	1 cut(s)	1675
BstZI	CGGCCG	1 cut(s)	174
Bsu36I	CCTNAGG	2 cut(s)	675, 1758
BsuI	GTATCC	2 cut(s)	2512, 2855
BsuRI	GGCC	4 cut(s)	28, 176, 680, 1595
BtgI	CCRYGG	1 cut(s)	103
BtrI	CACGTC	1 cut(s)	2068
BtsCI	GGATG	6 cut(s)	311, 754, 1315, 1360, 1885, 2323
BtsIMutI	CAGTG	5 cut(s)	322, 1677, 1860, 1971, 2930
BveI	ACCTGC	2 cut(s)	2225, 2800
Cac8I	GCNNGC	9 cut(s)	324, 467, 630, 1029, 1047, 1698, 2778, 2905, 3267
CaiI	CAGNNNCTG	1 cut(s)	545
CciI	TCATGA	1 cut(s)	1468
CfoI	GCGC	2 cut(s)	2296, 2455
Cfr13I	GGNCC	5 cut(s)	523, 958, 1253, 1427, 1805
CseI	GACGC	1 cut(s)	244
Csp6I	GTAC	2 cut(s)	1385, 2790
CviQI	GTAC	2 cut(s)	1385, 2790
DpnI	GATC	8 cut(s)	693, 1121, 1777, 2385, 2604, 2715, 2838, 2898
DpnII	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
DraI	TTTAAA	2 cut(s)	1210, 2380
EaeI	YGGCCR	1 cut(s)	174
EagI	CGGCCG	1 cut(s)	174
Eam1104I	CTCTTC	3 cut(s)	364, 390, 1047
EarI	CTCTTC	3 cut(s)	364, 390, 1047
EclXI	CGGCCG	1 cut(s)	174
Eco130I	CCWWGG	4 cut(s)	103, 1590, 2673, 2793
Eco31I	GGTCTC	1 cut(s)	3002
Eco32I	GATATC	1 cut(s)	2332
Eco47I	GGWCC	5 cut(s)	523, 958, 1253, 1427, 1805
Eco52I	CGGCCG	1 cut(s)	174
Eco57I	CTGAAG	3 cut(s)	375, 441, 784
Eco81I	CCTNAGG	2 cut(s)	675, 1758
EcoNI	CCTNNNNNAGG	1 cut(s)	2724
EcoO109I	RGGNCCY	2 cut(s)	523, 1253
EcoRII	CCWGG	3 cut(s)	211, 1551, 3108
EcoRV	GATATC	1 cut(s)	2332
EcoT14I	CCWWGG	4 cut(s)	103, 1590, 2673, 2793
EcoT22I	ATGCAT	2 cut(s)	1885, 2397
ErhI	CCWWGG	4 cut(s)	103, 1590, 2673, 2793
Esp3I	CGTCTC	2 cut(s)	1123, 1904
FalI	AAGNNNNNCTT	6 cut(s)	100, 132, 999, 1031, 2666, 2698
FaqI	GGGAC	1 cut(s)	299
FbaI	TGATCA	1 cut(s)	691
FblI	GTMKAC	3 cut(s)	1674, 2070, 2199
Fnu4HI	GCNGC	8 cut(s)	29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
FokI	GGATG	6 cut(s)	298, 761, 1322, 1367, 1892, 2310
Fsp4HI	GCNGC	8 cut(s)	29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
FspBI	CTAG	4 cut(s)	252, 432, 2297, 2355
GlaI	GCGC	2 cut(s)	2295, 2454
GluI	GCNGC	8 cut(s)	29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
GsaI	CCCAGC	2 cut(s)	245, 330
GsuI	CTGGAG	3 cut(s)	1535, 2817, 3072
HaeII	RGCGCY	1 cut(s)	2456
HaeIII	GGCC	4 cut(s)	28, 176, 680, 1595
HapII	CCGG	2 cut(s)	56, 746
HgaI	GACGC	1 cut(s)	244
HhaI	GCGC	2 cut(s)	2296, 2455
Hin1I	GRCGYC	1 cut(s)	3105
Hin6I	GCGC	2 cut(s)	2294, 2453
HinP1I	GCGC	2 cut(s)	2294, 2453
HincII	GTYRAC	5 cut(s)	361, 409, 558, 2071, 3082
HindII	GTYRAC	5 cut(s)	361, 409, 558, 2071, 3082
HindIII	AAGCTT	1 cut(s)	1246
HinfI	GANTC	8 cut(s)	268, 449, 863, 1282, 1988, 2032, 2223, 2760
HpaI	GTTAAC	1 cut(s)	558
HpaII	CCGG	2 cut(s)	56, 746
Hpy99I	CGWCG	1 cut(s)	2072
HpyCH4IV	ACGT	4 cut(s)	1182, 1585, 2067, 3105
HpySE526I	ACGT	4 cut(s)	1182, 1585, 2067, 3105
Hsp92I	GRCGYC	1 cut(s)	3105
HspAI	GCGC	2 cut(s)	2294, 2453
Ksp22I	TGATCA	1 cut(s)	691
KspAI	GTTAAC	1 cut(s)	558
Kzo9I	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
LguI	GCTCTTC	1 cut(s)	1047
Lsp1109I	GCAGC	7 cut(s)	1094, 1558, 2278, 2656, 3139, 3253, 3308
LweI	GCATC	8 cut(s)	26, 75, 910, 1870, 2251, 2560, 3139, 3228
MaeI	CTAG	4 cut(s)	252, 432, 2297, 2355
MaeII	ACGT	4 cut(s)	1182, 1585, 2067, 3105
MaeIII	GTNAC	7 cut(s)	1358, 1703, 1711, 1827, 1989, 2026, 2877
MalI	GATC	8 cut(s)	693, 1121, 1777, 2385, 2604, 2715, 2838, 2898
MbiI	CCGCTC	1 cut(s)	31
MboI	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
MfeI	CAATTG	2 cut(s)	477, 1320
MflI	RGATCY	2 cut(s)	2602, 2836
MhlI	GDGCHC	4 cut(s)	382, 1105, 2362, 2905
MlyI	GAGTC	3 cut(s)	872, 1997, 2041
MmeI	TCCRAC	9 cut(s)	76, 103, 567, 764, 1283, 1769, 1857, 1987, 2529
Mph1103I	ATGCAT	2 cut(s)	1885, 2397
MroXI	GAANNNNTTC	1 cut(s)	112
MslI	CAYNNNNRTG	6 cut(s)	704, 941, 1467, 1964, 2343, 3014
MspA1I	CMGCKG	1 cut(s)	2711
MspI	CCGG	2 cut(s)	56, 746
MspR9I	CCNGG	4 cut(s)	57, 213, 1553, 3110
MunI	CAATTG	2 cut(s)	477, 1320
MvaI	CCWGG	3 cut(s)	213, 1553, 3110
NciI	CCSGG	1 cut(s)	57
NcoI	CCATGG	1 cut(s)	103
NdeII	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
NlaIV	GGNNCC	5 cut(s)	766, 1793, 1806, 2211, 2604
NmuCI	GTSAC	3 cut(s)	1358, 1711, 1989
NsiI	ATGCAT	2 cut(s)	1885, 2397
NspI	RCATGY	3 cut(s)	20, 2395, 2780
NspV	TTCGAA	1 cut(s)	3043
PaeI	GCATGC	1 cut(s)	2780
PagI	TCATGA	1 cut(s)	1468
PciSI	GCTCTTC	1 cut(s)	1047
PdmI	GAANNNNTTC	1 cut(s)	112
PfeI	GAWTC	5 cut(s)	268, 449, 1282, 2223, 2760
PflMI	CCANNNNNTGG	1 cut(s)	1970
PfoI	TCCNGGA	1 cut(s)	1551
PkrI	GCNGC	8 cut(s)	30, 1084, 1548, 2293, 2671, 3154, 3243, 3298
PleI	GAGTC	3 cut(s)	871, 1996, 2040
PpsI	GAGTC	3 cut(s)	871, 1996, 2040
Ppu21I	YACGTR	1 cut(s)	1586
PpuMI	RGGWCCY	2 cut(s)	523, 1253
Psp5II	RGGWCCY	2 cut(s)	523, 1253
Psp6I	CCWGG	3 cut(s)	211, 1551, 3108
PspFI	CCCAGC	2 cut(s)	241, 326
PspGI	CCWGG	3 cut(s)	211, 1551, 3108
PspN4I	GGNNCC	5 cut(s)	766, 1793, 1806, 2211, 2604
PspPI	GGNCC	5 cut(s)	523, 958, 1253, 1427, 1805
PspPPI	RGGWCCY	2 cut(s)	523, 1253
PstI	CTGCAG	1 cut(s)	2542
PstNI	CAGNNNCTG	1 cut(s)	545
PsuI	RGATCY	2 cut(s)	2602, 2836
PvuII	CAGCTG	1 cut(s)	2711
RsaI	GTAC	2 cut(s)	1386, 2791
RsaNI	GTAC	2 cut(s)	1385, 2790
RseI	CAYNNNNRTG	6 cut(s)	704, 941, 1467, 1964, 2343, 3014
SalI	GTCGAC	1 cut(s)	2069
SapI	GCTCTTC	1 cut(s)	1047
SatI	GCNGC	8 cut(s)	29, 1083, 1547, 2292, 2670, 3153, 3242, 3297
Sau3AI	GATC	8 cut(s)	691, 1119, 1775, 2383, 2602, 2713, 2836, 2896
Sau96I	GGNCC	5 cut(s)	523, 958, 1253, 1427, 1805
SchI	GAGTC	3 cut(s)	872, 1997, 2041
ScrFI	CCNGG	4 cut(s)	57, 213, 1553, 3110
SduI	GDGCHC	4 cut(s)	382, 1105, 2362, 2905
SfaNI	GCATC	8 cut(s)	26, 75, 910, 1870, 2251, 2560, 3139, 3228
SfcI	CTRYAG	4 cut(s)	1173, 1482, 2538, 2721
SfuI	TTCGAA	1 cut(s)	3043
SinI	GGWCC	5 cut(s)	523, 958, 1253, 1427, 1805
SmiMI	CAYNNNNRTG	6 cut(s)	704, 941, 1467, 1964, 2343, 3014
SmlI	CTYRAG	4 cut(s)	646, 821, 986, 2441
SmoI	CTYRAG	4 cut(s)	646, 821, 986, 2441
SpeI	ACTAGT	1 cut(s)	431
SphI	GCATGC	1 cut(s)	2780
SsiI	CCGC	5 cut(s)	29, 1073, 2549, 2994, 3189
SspI	AATATT	2 cut(s)	280, 663
SspMI	CTAG	4 cut(s)	252, 432, 2297, 2355
StyD4I	CCNGG	4 cut(s)	55, 211, 1551, 3108
StyI	CCWWGG	4 cut(s)	103, 1590, 2673, 2793
TaiI	ACGT	4 cut(s)	1185, 1588, 2070, 3108
TaqI	TCGA	8 cut(s)	308, 444, 493, 2070, 3043, 3117, 3162, 3273
TaqII	GACCGA	1 cut(s)	2185
TauI	GCSGC	1 cut(s)	31
TfiI	GAWTC	5 cut(s)	268, 449, 1282, 2223, 2760
TscAI	CASTG	5 cut(s)	322, 1684, 1867, 1971, 2937
TseFI	GTSAC	3 cut(s)	1358, 1711, 1989
TseI	GCWGC	7 cut(s)	1082, 1546, 2291, 2669, 3152, 3241, 3296
Tsp45I	GTSAC	3 cut(s)	1358, 1711, 1989
TspGWI	ACGGA	2 cut(s)	1863, 1928
TspRI	CASTG	5 cut(s)	322, 1684, 1867, 1971, 2937
Van91I	CCANNNNNTGG	1 cut(s)	1970
VneI	GTGCAC	1 cut(s)	2358
VpaK11BI	GGWCC	5 cut(s)	523, 958, 1253, 1427, 1805
XagI	CCTNNNNNAGG	1 cut(s)	2724
XapI	RAATTY	2 cut(s)	2300, 2863
XceI	RCATGY	3 cut(s)	20, 2395, 2780
XcmI	CCANNNNNNNNNTGG	1 cut(s)	86
XmiI	GTMKAC	3 cut(s)	1674, 2070, 2199
XmnI	GAANNNNTTC	1 cut(s)	112
XspI	CTAG	4 cut(s)	252, 432, 2297, 2355
ZraI	GACGTC	1 cut(s)	3106
Zsp2I	ATGCAT	2 cut(s)	1885, 2397

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.