FvH4_3g02040

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003232
Physical Location & Seq
Reverse (-)
1023652 .. 1032269
8618 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02040.t1

Sequence Viewer

Length: 3276 bp
ATGGAGGTGTCTTTGCAAGCTCATAGGCCACTCAGTTGCAGAACTGTGTTCCAAGACAGAAGAACCAATCTCAAGCTCAAACCAGTTTCTGGGTTCTGTCCTCATGGAAGATATTCTTCATGGTTCAAAGGAGACCTGACAACTGGGGTTTCATGTAAAATTACTGCTAGTTCAGCTGATTTTTCTAGGAGACAGAGAAAAGTGTCGAGTTCAAGGCCTAATGGCCCTGGTCCAAAGGCAACAGTTCCCAAACCTCCAGTAGAGACTAGTGTCCCAAAAACAAGTCAAAGAAATACTGGAGACAAAAAGGGTTTTGCGTCTTCCACAGCCAGTGTTTCTGGGCCGAAAGTTGATGTAAAGAGGATTGATGAAACTAGTCGTAAAGTTGCGGAGTCTTCATCACTTAGCAAAACATCAGCAACTGGTAGAAGCTTTCAAGAGGTAGAGAATGGGAGTAGAGATAAAGGCATTGATATTGATAAGAAGCTATCTCATGAAGCTTCTTTGAAGTTAAAGTTGGAGATGGAAGAAAAACAACGTATGGAGGAGATTGAGAGGTTAGCTGAGGAAAACTTCTCCAAAGGGAACAAGCTCTTTGTCTATCCTCAAGTGGTGAAACCTGATCAACATATAGAAGTGTTTCTTAATAGAAGTCTTTCCACCCTAAACAGTGAAGATGATGTAGTCATTATGGGGGCTTTTAATGACTGGCGCTGGAAATCTTTTACCATTCGGTTGATCAGAACAAATCTCAAAGGGGATTGGTGGTCTTGTCAATTTCATGTTCCTGCCGAAGCATATAAGATAGATTTTGTGTTCTTCAATGGAAAAGATGTTTATGACAATAATGACAAGAAGGATTTCTGCATAGAAATAGAAAGTGGAATGGACGCCTATGCATTTGAAGATTTCTTACTCGAGGAGAAGCGTAAAGAACTTGAGAAACTTGCAGAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATCGAAGCAGAGAAGGCAGCAAGAGAATTTGACAGAGCAGAGGCAAAGGCAGAGACTGAAAGGAGACGGAAATTGGTGCAAGAGTTGGCCAAAAAGGCTGTAAGGTCAGTTCCAGATGTTTGGTACATAGAACCTCGGGAGTTTAAAGGTGAGGACGTGGTGAGGTTGTATTATAACCGAAGCTCAGGTCCTCTTGCTAATGCTAAGGAACTGTGGATTCATGGAGGACATAATGGTTGGAGTGCTGGCTTATCAATTATTGAAAGTCTTGTTAGGTCTGAGGAAAAGGACGGTGACTGGTGGTATGCCAAAGTTGTTGTACCTGAACAAGCTGTTGTATTGGATTGGGTTTTTGCCGATGGACCACCTCAGAATGCCACAGTATATGATAACAACCATCGTCATGATTTCCATGCAATAGTTCCGAAGTCTATTCCTGAGGAACAATATTGGGTTGAGGAAGAACACCAGATCTATAGGAAGCTCCAGGAGGAGAGGAGATTAAGGGAGGAGGCCATACGTGCCAAGGCCAAGAAAACAGCACTTATGAAAGCAGAAATGAAGGAACAAACTCTGAGAAGGTACCTGCTGTCTCAGAAGCATGTAGTCTACACTGAGCCCCTTGATGTCCAAGCTGGAAGCATGGTCACAATTTTCTACAATCCTGCCAATACTGTTCTTAACGGAAAGCCTGAGATTTGGTTCAGATGCTCCTTTAACCATTGGGCCCACCGCCGTGGTCCTTTGCCACCACAGAAAATGGTACCTGCAGAAAATGGTACCCATGTCAAAGCCACTGTTATGGTTCCACTCGATGCATACGTGATGGACTTTGTCTTTTCTGAGAGTGAAGAAGGCGGACTTTTTGATAACAAAAATGAAATGGATTATCACATTCCAGTGTTTGGAGGAGTTTCAAAGGAGTCACCAATGCACATTGTGCATATCACTGTTGAAATGGCACCCATTGCCAAGGTTGGAGGCCTTGGTGATGTGGTTACCAGTCTATCCCGAGCTGTACAAGACTTAAATCACCATGTCGACGTCATTCTTCCCAAGTATGACTGTTTGAACCTTAGCAATGTTAAGGAATTCCAGTATAACAGAACCTATTCTTGGGGTGGTACTGAAATCAAAGTTTGGTTTGGGAAAGTGGAAGGGGTTCCAGTCTACTTTCTGGAACCTCAAAATGGACTGTTTTATAAGGGTTGTATATATGGTTGTAAGAATGATTCGGAGAGATTTGGTTTCTTTTGCCATGCTGCTCTAGAATATCTACTCCAAAGTGGATCCCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCACCTGTTGCCTGGTTATATAAAGATCATTACTCGCATTATGGTCTTAGCAAAGCTCGCATTGTCTTCACAATTCACAATCTTGAATTTGGAGCGCACTATATTGGAAAAGCTGTGGCGTACTCAGACAAGTCTACTACTGTATCTGAGACTTATTCTAAGGAGATTGCTAGAAATCCTGCAGTAGCTCCGCACCTCTACAAGTTCCATGGCATAATAAACGGAATTGATCAAGATATATGGGACCCATACAATGATGGTTTCCTACCTATATCATATACTTCTGAAAATGTTATTGAAGGCAAAAAAGCTGCTAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGCAGATCTCCCTTTAGTAGGTATTATTACTCGCTTAACTCAGCAGAAAGGAATCCATCTTATCAAGCATGCCATTTGGCGTACCTTGGAACGCAATGGACAGGTTGTATTGCTTGGTTCAGCTCCAGATCCTCGTATCCAAAATGACTTTGTAAATTTGGCGAATCAATTACATTCTTCTCACGGTGATCGTGCTCGTCTTTGTTTGACTTATGATGAGCCACTCTCACATTTGATATACGCTGGTTCTGATTTCATCCTTGTTCCTTCAATATTTGAGCCATGTGGACTGACCCAACTTGTAGCAATGAGATACGGTTCCATACCTGTTGTTCGAAAAACCGGAGGGCTCTATGACACTGTGTTTGATGTTGACCATGATAAAGAGAGAGCAGAGGCGCAGGGTCTTGAACCAAATGGGTTCAGTTTCGATGGAGCTGATGCTGCTGGTGTTGATTATGCTCTAAATAGGGCTATCTCTGCCTGGTATGACGGTCAAGATTGGTTCAACTCGTTGTGCAAGACAGTGATGGAGCAAGATTGGTCTTGGAATCGGCCAGCTCTTGAATATATGGAGTTGTATCACGCAGCACGGAAGTGA

Protein Analysis

1092

Amino Acids

124.26

Weight (kDa)

6.46

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 212 - 271 3.5e-10 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 386 - 448 1.4e-13 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 548 - 610 2e-10 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 644 - 834 1.3e-48 Starch synthase catalytic domain
Glyco_transf_4 PF13439 658 - 839 2.5e-09 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1170, 2199
AatII GACGTC 1 cut(s) 2043
Acc36I ACCTGC 2 cut(s) 1590, 1771
Acc65I GGTACC 3 cut(s) 1578, 1759, 1775
AccB1I GGYRCC 4 cut(s) 1578, 1759, 1775, 1957
AccB7I CCANNNNNTGG 2 cut(s) 89, 1901
AccI GTMKAC 4 cut(s) 1605, 2037, 2166, 2459
AciI CCGC 4 cut(s) 389, 1729, 1854, 2516
AclWI GGATC 3 cut(s) 2280, 2293, 2798
AcoI YGGCCR 2 cut(s) 1083, 3230
AcsI RAATTY 4 cut(s) 1022, 2087, 2411, 2830
AcyI GRCGYC 2 cut(s) 891, 2040
AdeI CACNNNGTG 1 cut(s) 1936
AfaI GTAC 9 cut(s) 1121, 1317, 1580, 1761, 1777, 2016, 2122, 2447, 2758
AfiI CCNNNNNNNGG 5 cut(s) 89, 1901, 2112, 2186, 2693
AhlI ACTAGT 2 cut(s) 266, 374
AjiI CACGTC 1 cut(s) 1153
AjnI CCWGG 4 cut(s) 226, 1482, 2336, 3158
AjuI GAANNNNNNNTTGG 4 cut(s) 500, 532, 2095, 2127
AleI CACNNNNGTG 2 cut(s) 1731, 3271
Alw21I GWGCWC 2 cut(s) 2329, 2872
Alw26I GTCTC 8 cut(s) 126, 184, 257, 294, 1043, 1054, 1593, 2468
Alw44I GTGCAC 1 cut(s) 2325
AlwI GGATC 3 cut(s) 2280, 2293, 2798
AlwNI CAGNNNCTG 3 cut(s) 89, 422, 1052
Ama87I CYCGRG 3 cut(s) 917, 1131, 2007
ApaI GGGCCC 1 cut(s) 1726
ApaLI GTGCAC 1 cut(s) 2325
ApeKI GCWGC 5 cut(s) 1013, 2258, 2636, 3119, 3263
ApoI RAATTY 4 cut(s) 1022, 2087, 2411, 2830
ArsI GACNNNNNNTTYG 6 cut(s) 1168, 1200, 2650, 2682, 3023, 3055
Asp700I GAANNNNTTC 6 cut(s) 112, 639, 655, 860, 2107, 2157
Asp718I GGTACC 3 cut(s) 1578, 1759, 1775
AspLEI GCGC 3 cut(s) 714, 2422, 3076
AspS9I GGNCC 9 cut(s) 224, 230, 341, 1184, 1358, 1722, 1723, 1736, 2569
AsuHPI GGTGA 8 cut(s) 625, 1157, 1168, 1301, 1914, 1997, 2021, 2873
AsuII TTCGAA 1 cut(s) 3010
AvaI CYCGRG 3 cut(s) 917, 1131, 2007
AvaII GGWCC 5 cut(s) 230, 1184, 1358, 1736, 2569
AxyI CCTNAGG 1 cut(s) 1434
BaeGI GKGCMC 2 cut(s) 1726, 2329
BaeI ACNNNNGTAYC 4 cut(s) 1767, 1800, 2451, 2484
BalI TGGCCA 1 cut(s) 1085
BamHI GGATCC 1 cut(s) 2285
BanI GGYRCC 4 cut(s) 1578, 1759, 1775, 1957
BanII GRGCYC 3 cut(s) 1617, 1726, 3027
BarI GAAGNNNNNNTAC 2 cut(s) 897, 929
BbsI GAAGAC 3 cut(s) 312, 387, 2383
Bbv12I GWGCWC 2 cut(s) 2329, 2872
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 4 cut(s) 1025, 2245, 2623, 3106
BccI CCATC 9 cut(s) 517, 1349, 1401, 1816, 2298, 2576, 2739, 3101, 3199
BceAI ACGGC 1 cut(s) 1716
BciT130I CCWGG 4 cut(s) 228, 1484, 2338, 3160
BciVI GTATCC 1 cut(s) 2822
BclI TGATCA 3 cut(s) 622, 738, 2554
BcoDI GTCTC 8 cut(s) 126, 184, 257, 294, 1043, 1054, 1593, 2468
BcuI ACTAGT 2 cut(s) 266, 374
BfaI CTAG 7 cut(s) 168, 186, 267, 375, 2264, 2322, 2496
BfmI CTRYAG 3 cut(s) 1471, 1764, 2505
BfoI RGCGCY 1 cut(s) 715
BfuAI ACCTGC 2 cut(s) 1590, 1771
BfuI GTATCC 1 cut(s) 2822
BglI GCCNNNNNGGC 1 cut(s) 1091
BglII AGATCT 2 cut(s) 1467, 2680
BisI GCNGC 5 cut(s) 1014, 2259, 2637, 3120, 3264
BlsI GCNGC 5 cut(s) 1015, 2260, 2638, 3121, 3265
Bme1390I CCNGG 4 cut(s) 228, 1484, 2338, 3160
Bme18I GGWCC 5 cut(s) 230, 1184, 1358, 1736, 2569
BmeT110I CYCGRG 3 cut(s) 917, 1131, 2007
BmgBI CACGTC 1 cut(s) 1153
BmgT120I GGNCC 9 cut(s) 224, 230, 341, 1184, 1358, 1722, 1723, 1736, 2569
BmrFI CCNGG 4 cut(s) 228, 1484, 2338, 3160
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 3 cut(s) 1694, 1801, 3106
BmuI ACTGGG 1 cut(s) 153
BoxI GACNNNNGTC 1 cut(s) 269
BpiI GAAGAC 3 cut(s) 312, 387, 2383
BplI GAGNNNNNCTC 2 cut(s) 2885, 2917
BpmI CTGGAG 4 cut(s) 240, 318, 1466, 2784
Bpu10I CCTNAGC 5 cut(s) 564, 1180, 1200, 2072, 2640
Bpu14I TTCGAA 1 cut(s) 3010
BpuEI CTTGAG 3 cut(s) 56, 591, 959
BsaAI YACGTR 2 cut(s) 1517, 1819
BsaHI GRCGYC 2 cut(s) 891, 2040
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 8 cut(s) 226, 1130, 1521, 1732, 1968, 1981, 2533, 2760
BsaWI WCCGGW 1 cut(s) 3017
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 1901, 2112, 2186, 2693
Bse21I CCTNAGG 1 cut(s) 1434
Bse3DI GCAATG 5 cut(s) 1962, 2083, 2305, 2776, 2988
BseBI CCWGG 4 cut(s) 228, 1484, 2338, 3160
BseDI CCNNGG 8 cut(s) 226, 1130, 1521, 1732, 1968, 1981, 2533, 2760
BseGI GGATG 2 cut(s) 2290, 2931
BseLI CCNNNNNNNGG 5 cut(s) 89, 1901, 2112, 2186, 2693
BseMI GCAATG 5 cut(s) 1962, 2083, 2305, 2776, 2988
BseRI GAGGAG 7 cut(s) 560, 935, 968, 1502, 1507, 1520, 1920
BseSI GKGCMC 2 cut(s) 1726, 2329
BseXI GCAGC 4 cut(s) 1025, 2245, 2623, 3106
BshNI GGYRCC 4 cut(s) 1578, 1759, 1775, 1957
BsiHKAI GWGCWC 2 cut(s) 2329, 2872
BsiHKCI CYCGRG 3 cut(s) 917, 1131, 2007
BsiSI CCGG 1 cut(s) 3018
BslFI GGGAC 2 cut(s) 257, 2582
BslI CCNNNNNNNGG 5 cut(s) 89, 1901, 2112, 2186, 2693
BsmAI GTCTC 8 cut(s) 126, 184, 257, 294, 1043, 1054, 1593, 2468
BsmBI CGTCTC 1 cut(s) 1054
BsmFI GGGAC 2 cut(s) 257, 2582
BsmI GAATGC 1 cut(s) 1375
Bso31I GGTCTC 1 cut(s) 126
BsoBI CYCGRG 3 cut(s) 917, 1131, 2007
Bsp119I TTCGAA 1 cut(s) 3010
Bsp120I GGGCCC 1 cut(s) 1722
Bsp1286I GDGCHC 5 cut(s) 1617, 1726, 2329, 2872, 3027
Bsp1407I TGTACA 1 cut(s) 2014
Bsp143I GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
Bsp19I CCATGG 1 cut(s) 2533
BspACI CCGC 4 cut(s) 389, 1729, 1854, 2516
BspHI TCATGA 2 cut(s) 493, 1399
BspMAI CTGCAG 2 cut(s) 1768, 2509
BspMI ACCTGC 2 cut(s) 1590, 1771
BspPI GGATC 3 cut(s) 2280, 2293, 2798
BspQI GCTCTTC 1 cut(s) 978
BspT104I TTCGAA 1 cut(s) 3010
BspT107I GGYRCC 4 cut(s) 1578, 1759, 1775, 1957
BspTNI GGTCTC 1 cut(s) 126
BsrDI GCAATG 5 cut(s) 1962, 2083, 2305, 2776, 2988
BsrGI TGTACA 1 cut(s) 2014
BssECI CCNNGG 8 cut(s) 226, 1130, 1521, 1732, 1968, 1981, 2533, 2760
BssMI GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
BssNI GRCGYC 2 cut(s) 891, 2040
BssT1I CCWWGG 5 cut(s) 1521, 1968, 1981, 2533, 2760
Bst2UI CCWGG 4 cut(s) 228, 1484, 2338, 3160
Bst6I CTCTTC 1 cut(s) 978
BstACI GRCGYC 2 cut(s) 891, 2040
BstAPI GCANNNNNTGC 3 cut(s) 1936, 1964, 2333
BstAUI TGTACA 1 cut(s) 2014
BstBAI YACGTR 2 cut(s) 1517, 1819
BstBI TTCGAA 1 cut(s) 3010
BstC8I GCNNGC 7 cut(s) 18, 960, 978, 1243, 2383, 2745, 3234
BstDSI CCRYGG 2 cut(s) 1732, 2533
BstEII GGTNACC 1 cut(s) 1993
BstENI CCTNNNNNAGG 1 cut(s) 2691
BstF5I GGATG 2 cut(s) 2290, 2931
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 3 cut(s) 714, 2422, 3076
BstKTI GATC 9 cut(s) 625, 741, 1470, 2288, 2353, 2557, 2683, 2806, 2866
BstMAI GTCTC 8 cut(s) 126, 184, 257, 294, 1043, 1054, 1593, 2468
BstMBI GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
BstNI CCWGG 4 cut(s) 228, 1484, 2338, 3160
BstNSI RCATGY 2 cut(s) 1601, 2747
BstPAI GACNNNNGTC 1 cut(s) 269
BstPI GGTNACC 1 cut(s) 1993
BstSCI CCNGG 4 cut(s) 226, 1482, 2336, 3158
BstSFI CTRYAG 3 cut(s) 1471, 1764, 2505
BstSLI GKGCMC 2 cut(s) 1726, 2329
BstV1I GCAGC 4 cut(s) 1025, 2245, 2623, 3106
BstV2I GAAGAC 3 cut(s) 312, 387, 2383
BstX2I RGATCY 4 cut(s) 1467, 2285, 2680, 2803
BstXI CCANNNNNNTGG 3 cut(s) 1116, 1733, 1798
BstYI RGATCY 4 cut(s) 1467, 2285, 2680, 2803
Bsu36I CCTNAGG 1 cut(s) 1434
BsuI GTATCC 1 cut(s) 2822
BtgI CCRYGG 2 cut(s) 1732, 2533
BtrI CACGTC 1 cut(s) 1153
BtsCI GGATG 2 cut(s) 2290, 2931
BtsIMutI CAGTG 8 cut(s) 337, 676, 1608, 1791, 1902, 1944, 3033, 3207
BveI ACCTGC 2 cut(s) 1590, 1771
Cac8I GCNNGC 7 cut(s) 18, 960, 978, 1243, 2383, 2745, 3234
CaiI CAGNNNCTG 3 cut(s) 89, 422, 1052
CciI TCATGA 2 cut(s) 493, 1399
CfoI GCGC 3 cut(s) 714, 2422, 3076
Cfr13I GGNCC 9 cut(s) 224, 230, 341, 1184, 1358, 1722, 1723, 1736, 2569
CseI GACGC 2 cut(s) 306, 899
Csp6I GTAC 9 cut(s) 1120, 1316, 1579, 1760, 1776, 2015, 2121, 2446, 2757
CviQI GTAC 9 cut(s) 1120, 1316, 1579, 1760, 1776, 2015, 2121, 2446, 2757
DpnI GATC 9 cut(s) 624, 740, 1469, 2287, 2352, 2556, 2682, 2805, 2865
DpnII GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
DraI TTTAAA 1 cut(s) 1141
DraIII CACNNNGTG 1 cut(s) 1936
EaeI YGGCCR 2 cut(s) 1083, 3230
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
EciI GGCGGA 1 cut(s) 1869
Eco130I CCWWGG 5 cut(s) 1521, 1968, 1981, 2533, 2760
Eco147I AGGCCT 2 cut(s) 217, 1980
Eco24I GRGCYC 3 cut(s) 1617, 1726, 3027
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 1 cut(s) 2299
Eco47I GGWCC 5 cut(s) 230, 1184, 1358, 1736, 2569
Eco81I CCTNAGG 1 cut(s) 1434
Eco88I CYCGRG 3 cut(s) 917, 1131, 2007
Eco91I GGTNACC 1 cut(s) 1993
EcoNI CCTNNNNNAGG 1 cut(s) 2691
EcoO109I RGGNCCY 2 cut(s) 1184, 2569
EcoO65I GGTNACC 1 cut(s) 1993
EcoRI GAATTC 1 cut(s) 2087
EcoRII CCWGG 4 cut(s) 226, 1482, 2336, 3158
EcoRV GATATC 1 cut(s) 2299
EcoT14I CCWWGG 5 cut(s) 1521, 1968, 1981, 2533, 2760
EcoT22I ATGCAT 2 cut(s) 901, 1816
EcoT38I GRGCYC 3 cut(s) 1617, 1726, 3027
ErhI CCWWGG 5 cut(s) 1521, 1968, 1981, 2533, 2760
Esp3I CGTCTC 1 cut(s) 1054
FalI AAGNNNNNCTT 8 cut(s) 100, 132, 627, 659, 1842, 1874, 2633, 2665
FaqI GGGAC 2 cut(s) 257, 2582
FbaI TGATCA 3 cut(s) 622, 738, 2554
FblI GTMKAC 4 cut(s) 1605, 2037, 2166, 2459
Fnu4HI GCNGC 5 cut(s) 1014, 2259, 2637, 3120, 3264
FokI GGATG 2 cut(s) 2277, 2918
FriOI GRGCYC 3 cut(s) 1617, 1726, 3027
Fsp4HI GCNGC 5 cut(s) 1014, 2259, 2637, 3120, 3264
FspBI CTAG 7 cut(s) 168, 186, 267, 375, 2264, 2322, 2496
GlaI GCGC 3 cut(s) 713, 2421, 3075
GluI GCNGC 5 cut(s) 1014, 2259, 2637, 3120, 3264
GsuI CTGGAG 4 cut(s) 240, 318, 1466, 2784
HaeII RGCGCY 1 cut(s) 715
HapII CCGG 1 cut(s) 3018
HgaI GACGC 2 cut(s) 306, 899
HhaI GCGC 3 cut(s) 714, 2422, 3076
Hin1I GRCGYC 2 cut(s) 891, 2040
Hin6I GCGC 3 cut(s) 712, 2420, 3074
HinP1I GCGC 3 cut(s) 712, 2420, 3074
HincII GTYRAC 2 cut(s) 2038, 3049
HindII GTYRAC 2 cut(s) 2038, 3049
HindIII AAGCTT 2 cut(s) 430, 498
HinfI GANTC 8 cut(s) 392, 996, 1213, 1919, 2228, 2727, 2839, 3226
HpaII CCGG 1 cut(s) 3018
HphI GGTGA 8 cut(s) 625, 1157, 1168, 1301, 1914, 1997, 2021, 2873
Hpy166II GTNNAC 7 cut(s) 1606, 2038, 2167, 2327, 2460, 2963, 3049
Hpy8I GTNNAC 7 cut(s) 1606, 2038, 2167, 2327, 2460, 2963, 3049
Hpy99I CGWCG 1 cut(s) 2042
HpyAV CCTTC 8 cut(s) 850, 1003, 1552, 1569, 1844, 2147, 2618, 2952
HpyCH4IV ACGT 5 cut(s) 538, 1152, 1516, 1818, 2040
HpySE526I ACGT 5 cut(s) 538, 1152, 1516, 1818, 2040
Hsp92I GRCGYC 2 cut(s) 891, 2040
HspAI GCGC 3 cut(s) 712, 2420, 3074
KflI GGGWCCC 1 cut(s) 2569
KpnI GGTACC 3 cut(s) 1582, 1763, 1779
Ksp22I TGATCA 3 cut(s) 622, 738, 2554
Kzo9I GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 8 cut(s) 955, 1485, 1712, 2417, 2518, 2803, 3110, 3208
Lsp1109I GCAGC 4 cut(s) 1025, 2245, 2623, 3106
LweI GCATC 3 cut(s) 1694, 1801, 3106
MaeI CTAG 7 cut(s) 168, 186, 267, 375, 2264, 2322, 2496
MaeII ACGT 5 cut(s) 538, 1152, 1516, 1818, 2040
MaeIII GTNAC 4 cut(s) 1289, 1642, 1920, 1993
MalI GATC 9 cut(s) 624, 740, 1469, 2287, 2352, 2556, 2682, 2805, 2865
MboI GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
MflI RGATCY 4 cut(s) 1467, 2285, 2680, 2803
MhlI GDGCHC 5 cut(s) 1617, 1726, 2329, 2872, 3027
MlsI TGGCCA 1 cut(s) 1085
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 2 cut(s) 401, 1928
MmeI TCCRAC 3 cut(s) 498, 1214, 1954
Mox20I TGGCCA 1 cut(s) 1085
Mph1103I ATGCAT 2 cut(s) 901, 1816
MroXI GAANNNNTTC 6 cut(s) 112, 639, 655, 860, 2107, 2157
MscI TGGCCA 1 cut(s) 1085
MslI CAYNNNNRTG 6 cut(s) 1398, 1731, 1796, 1895, 2310, 3271
Msp20I TGGCCA 1 cut(s) 1085
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 1 cut(s) 3018
MspR9I CCNGG 4 cut(s) 228, 1484, 2338, 3160
Mva1269I GAATGC 1 cut(s) 1375
MvaI CCWGG 4 cut(s) 228, 1484, 2338, 3160
NcoI CCATGG 1 cut(s) 2533
NdeII GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
NmuCI GTSAC 3 cut(s) 1289, 1642, 1920
NsiI ATGCAT 2 cut(s) 901, 1816
NspI RCATGY 2 cut(s) 1601, 2747
NspV TTCGAA 1 cut(s) 3010
OliI CACNNNNGTG 2 cut(s) 1731, 3271
PaeI GCATGC 1 cut(s) 2747
PaeR7I CTCGAG 1 cut(s) 917
PagI TCATGA 2 cut(s) 493, 1399
PceI AGGCCT 2 cut(s) 217, 1980
PciSI GCTCTTC 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 1815
PctI GAATGC 1 cut(s) 1375
PdmI GAANNNNTTC 6 cut(s) 112, 639, 655, 860, 2107, 2157
PfeI GAWTC 6 cut(s) 996, 1213, 2228, 2727, 2839, 3226
PflFI GACNNNGTC 1 cut(s) 1829
PflMI CCANNNNNTGG 2 cut(s) 89, 1901
PfoI TCCNGGA 1 cut(s) 1482
PkrI GCNGC 5 cut(s) 1015, 2260, 2638, 3121, 3265
PleI GAGTC 2 cut(s) 400, 1927
PpsI GAGTC 2 cut(s) 400, 1927
Ppu21I YACGTR 2 cut(s) 1517, 1819
PpuMI RGGWCCY 2 cut(s) 1184, 2569
PshAI GACNNNNGTC 1 cut(s) 269
PsiI TTATAA 2 cut(s) 1170, 2199
Psp5II RGGWCCY 2 cut(s) 1184, 2569
Psp6I CCWGG 4 cut(s) 226, 1482, 2336, 3158
PspEI GGTNACC 1 cut(s) 1993
PspGI CCWGG 4 cut(s) 226, 1482, 2336, 3158
PspOMI GGGCCC 1 cut(s) 1722
PspPI GGNCC 9 cut(s) 224, 230, 341, 1184, 1358, 1722, 1723, 1736, 2569
PspPPI RGGWCCY 2 cut(s) 1184, 2569
PspXI VCTCGAGB 1 cut(s) 917
PsrI GAACNNNNNNTAC 2 cut(s) 1089, 1121
PstI CTGCAG 2 cut(s) 1768, 2509
PstNI CAGNNNCTG 3 cut(s) 89, 422, 1052
PsuI RGATCY 4 cut(s) 1467, 2285, 2680, 2803
PsyI GACNNNGTC 1 cut(s) 1829
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 9 cut(s) 1121, 1317, 1580, 1761, 1777, 2016, 2122, 2447, 2758
RsaNI GTAC 9 cut(s) 1120, 1316, 1579, 1760, 1776, 2015, 2121, 2446, 2757
RseI CAYNNNNRTG 6 cut(s) 1398, 1731, 1796, 1895, 2310, 3271
SalI GTCGAC 1 cut(s) 2036
SapI GCTCTTC 1 cut(s) 978
SatI GCNGC 5 cut(s) 1014, 2259, 2637, 3120, 3264
Sau3AI GATC 9 cut(s) 622, 738, 1467, 2285, 2350, 2554, 2680, 2803, 2863
Sau96I GGNCC 9 cut(s) 224, 230, 341, 1184, 1358, 1722, 1723, 1736, 2569
SchI GAGTC 2 cut(s) 401, 1928
ScrFI CCNGG 4 cut(s) 228, 1484, 2338, 3160
SduI GDGCHC 5 cut(s) 1617, 1726, 2329, 2872, 3027
SfaNI GCATC 3 cut(s) 1694, 1801, 3106
SfcI CTRYAG 3 cut(s) 1471, 1764, 2505
Sfr274I CTCGAG 1 cut(s) 917
SfuI TTCGAA 1 cut(s) 3010
SinI GGWCC 5 cut(s) 230, 1184, 1358, 1736, 2569
SlaI CTCGAG 1 cut(s) 917
SmiMI CAYNNNNRTG 6 cut(s) 1398, 1731, 1796, 1895, 2310, 3271
SmlI CTYRAG 4 cut(s) 71, 606, 917, 938
SmoI CTYRAG 4 cut(s) 71, 606, 917, 938
SpeI ACTAGT 2 cut(s) 266, 374
SphI GCATGC 1 cut(s) 2747
SseBI AGGCCT 2 cut(s) 217, 1980
SsiI CCGC 4 cut(s) 389, 1729, 1854, 2516
SspI AATATT 2 cut(s) 1445, 2949
SspMI CTAG 7 cut(s) 168, 186, 267, 375, 2264, 2322, 2496
StuI AGGCCT 2 cut(s) 217, 1980
StyD4I CCNGG 4 cut(s) 226, 1482, 2336, 3158
StyI CCWWGG 5 cut(s) 1521, 1968, 1981, 2533, 2760
TaiI ACGT 5 cut(s) 541, 1155, 1519, 1821, 2043
TaqI TCGA 7 cut(s) 206, 918, 999, 1809, 2037, 3010, 3105
TatI WGTACW 1 cut(s) 2014
TfiI GAWTC 6 cut(s) 996, 1213, 2228, 2727, 2839, 3226
TscAI CASTG 8 cut(s) 337, 676, 1615, 1798, 1902, 1951, 3040, 3207
TseFI GTSAC 3 cut(s) 1289, 1642, 1920
TseI GCWGC 5 cut(s) 1013, 2258, 2636, 3119, 3263
Tsp45I GTSAC 3 cut(s) 1289, 1642, 1920
TspGWI ACGGA 3 cut(s) 1078, 1695, 2562
TspRI CASTG 8 cut(s) 337, 676, 1615, 1798, 1902, 1951, 3040, 3207
Tth111I GACNNNGTC 1 cut(s) 1829
Van91I CCANNNNNTGG 2 cut(s) 89, 1901
VneI GTGCAC 1 cut(s) 2325
VpaK11BI GGWCC 5 cut(s) 230, 1184, 1358, 1736, 2569
XagI CCTNNNNNAGG 1 cut(s) 2691
XapI RAATTY 4 cut(s) 1022, 2087, 2411, 2830
XbaI TCTAGA 1 cut(s) 2263
XceI RCATGY 2 cut(s) 1601, 2747
XhoI CTCGAG 1 cut(s) 917
XmiI GTMKAC 4 cut(s) 1605, 2037, 2166, 2459
XmnI GAANNNNTTC 6 cut(s) 112, 639, 655, 860, 2107, 2157
XspI CTAG 7 cut(s) 168, 186, 267, 375, 2264, 2322, 2496
ZraI GACGTC 1 cut(s) 2041
Zsp2I ATGCAT 2 cut(s) 901, 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.