MD05G1347400.v1.1

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003232
Physical Location & Seq
Forward (+)
46625246 .. 46632242
6997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1347400.v1.1.491

Sequence Viewer

Length: 3342 bp
ATGGAGGTTTCTGTACAAGCACAGAGGCCGCTCAGTTGCAGAGGACTTTTCCAACCGAGAAGGACCATCTTAAATCTCCAACCTTTTACTGGGTTCTTTCCCCATCGAAGGTATTCTTCATGGTTCAAAGGAAACCTGACAAGTGGGGTTTCATACCAAATTACTGCCAGTTCCGCCGAGTTTTCAAGGAGGAGACAAAGAAAAGTGTCTCCCGCTTCAAAGGGATTAGTGCCAGAAACCCCGGCAGGAACTAGCGTCCAGAAGACGAATCAAAGGAATACTGGGGACGAAAAGGGTATGACATCTTTGGCATCCAGTGAGCTTGCTGGGAAAAATAAGAAAACACATCAATCGAGGGTTGACACCAGAAGAGAATGGGCAGTTGAACCTTCAGAAGAGAGCAAAGTTGACGAAAAAAGAATTGATGAAACTAGTAGTAAAGTCGAGGAATCACCTTCAGTTGGCAAGCAGACAGCAATTGATAGGGGTATTCGAGGGATAGAAAATGGGTGTGTAGGTAAGGCCCTTGATGACATCTCAGATACTGATAAAGGCATTGATACTGATAAAAAGTTAACGAATGAAGATCCTTTAAAGTTAAAGTTGGAGAGGGAAGAAAAATTGCGTAAAGAGGAAATTGCGAGGCTTGCTGAGGAAAACTTCTCAAGAGGGAACAAAATATTTTTCTATCCTCAGGTGGTGAAACCTGATCAAGACATAGAAGTGTTCCTTAATAGAAGTATATCAACCCTAAGCAATGAGCCGGATGTTCAAATAATGGGAGCCTTTAATGACTGGCGTTGGAAATCTTTTACCTTCAGGTTGAACAAAACACAACTCAAAGGGGATTGGTGGTCTTGCCAGTTTCATGTTCCTAAAGAGTCATATAAAATAGACTTTGTGTTCTTTAATGGGCAAAATGTTTATGACAATAATGATGCTAAAGATTTCTGCATAACTGTGGAAGGTGGAATGGACCTCTTTGCATTTGAAGATTTCTTGCTTGAGGAGAAGCGTAAAGAACAAGAGAAACTTGCGAAGGAGCAAGCTGAGAGGGAAAGGCAGGCAGAAGAGCAGAGGCGAATTGAAGCGGAGAAGGCAGCAAGTGAAGCTGACAGAGCACAGGCAAAGGCAGAGATCATAAAGAGACGACAAATGGTGCAAGAGTTGATAAAAAAGGCTGTAAGGTCTGTAGAAGACGTTTGGTACATAGAGCCTAAAGAGTTTAAAGGTGAGGACTTGGTGAAGTTATACTATAACCGAGGCTCAGGTCCTCTTGCTAATGCTAAGGAACTATGGATTCATGGGGGGCATAATGGTTGGAAGGATGGATTGTCAATTGTTGAAAGGCTTGTTAGCTCTGAGAGAAAGGATGGTGACTGGTGGTATGCCAATGTTGTTGTACCTGAACAAGCTATTGTCTTGGATTGGGTTTTTGCTGATGGACCACCCCAGAATGCCGTTTTATATGATAACAATCACCGTCATGATTTCCATGCTATAGTTCCAAAATCCATTCCTGAGGAACTATATTGGGTTGAGGAAGAACACCAGATATATAAGAAGCTCCAGGAGGAGAGGAGATTAAGGGAGGAGGCAATACGTGCCAAGGCTGAAAGAACAGCTCGTATGAAAGCAGAAATGAAGGAAAGAACTTTCAAAAGGTTTTTGCTGTCTCAGAAGCATATAGTATACACTGAGCCTCTTGATGTTCAAGCTGGTAACAAGGTGACAGTTTTCTACAATCCTGCCAACACAGTTCTGAATGGAAAGCCTGAGGTTTGGTTGAGAGGATCATTTAACCGTTGGACCCACCGTAAGGGTCCATTGCAACCACAGAAGATGTTACCTACAGAGAATGGTTCTCATGTCAAAACCACTGTTTCAGTTCCGCTGGATGCATACATGATGGACTTTGTTTTCTCTGAGACGGAAGATGGTGGACTTTTTGACAACAAAAATGGCATGGATTACCACATTCCAGTGTTTGGAGGATTGCTTAAGGAGTCACCAATGAACATTGTGCATATTTCTGTTGGAGGTCTTGGTGATGTGGTTACGAGTCTCTCACGAGCTGTACAAGACTTAAATCACCACGTTGACATCATTCTTCCAAAGTATGACTGTTTGAACCTTAGCAATGTGAAGGAGTTTCAGTATAAAAGAGGCTTCTCTTGGGGAGGAACTGAAATAAAAGTTTGGTTTGGGAAAGTGGAAGGTGTTTCAGTCTACTTTTTGGAGCCTCAAAATGGATTCTTTCATGCAGGTTGCATATATGGTTGTAAAAATGATGCAGAGAGATTTGGTTTCTTTTGCCATGCTGCGCTAGAATTTCTACTCCAAAGTGGATTTCATCCTGATATCATTCATTGCCATGATTGGTCAAGTGCACCTGTTGCGTGGTTATTTAAAGATCATTACATGCATTATGGTCTTAGTAAAGCTCGGGTTGTTTTCACAATTCATAACCTTGAGTTTGGAGCGCCCTTCATTGGTAAAGCTGTGGCATATTCAGACAAGTCCACGACTGTATCCAACACTTATGCTAAGGAGGTATCTGGAAATCCTGCAGTTGCTCCGCATCTTTACAAGTTTCATGGTATAATAAATGGAATTGACCAAGATATATGGGATCCATACAATGATAAGTTCATTCCTATATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAAAAGCTGATCTCCCTGTAGTAGGTATTATTACTCGCTTAACTCACCAGAAAGGAATCCATCTCATCAAGCATGCCATTTGGCGTACCTTGGAACGCAATGGGCAGGTTGTATTGCTTGGTTCAGCTCCAGATCCTCGTATCCAAAATGATTTTGTAAATTTGGCAAATCAGTTACATTCTTCTCATGCTGATCGTGCTCGCCTCTGTTTGACTTACGATGAGCCACTGTCGCATTTGATATACGCTGGTGCTGATTTCATCTTAGTTCCTTCAATTTTTGAGCCGTGCGGTCTCACCCAACTCATAGCAATGAGATATGGTTCAATACCTGTTGTTCGAAAAACCGGAGGACTTTATGATACGGTATTTGATGTTGACCATGATAAAGAGAGAGCAAACGCCCAGGGTGTCGAGCCAAATGGTTTCAGCTTTGATGGAGCTGATGCTGCTGGTGTTGATTATGCTCTTAATAGGGCAATCTCCGCTTGGTATGATGGTCGGGATTGGTATAACTCGCTGTGCAAGACGGTGATGCAGCAAGATTGGTCTTGGAACAAGCCAGCTCTCGACTATATGGAGCTTTACCACGCAGCACGGAAGGGGTGA

Protein Analysis

1114

Amino Acids

126.93

Weight (kDa)

6.67

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 241 - 300 8.6e-10 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 415 - 477 2.1e-14 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 577 - 639 2.4e-13 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 677 - 853 9.1e-43 Starch synthase catalytic domain
Glyco_transf_4 PF13439 679 - 854 1.1e-08 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2255, 2830
AccB7I CCANNNNNTGG 1 cut(s) 1988
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 1692, 2229
AciI CCGC 8 cut(s) 29, 174, 213, 1091, 1892, 2579, 3024, 3219
AclWI GGATC 5 cut(s) 581, 1801, 2627, 2640, 2861
AcsI RAATTY 2 cut(s) 2330, 2893
AcuI CTGAAG 3 cut(s) 375, 441, 802
AfaI GTAC 5 cut(s) 15, 1208, 1404, 2079, 2821
AfiI CCNNNNNNNGG 8 cut(s) 89, 108, 461, 1819, 1988, 2249, 2492, 2756
AflII CTTAAG 1 cut(s) 2000
AhlI ACTAGT 1 cut(s) 431
AjnI CCWGG 2 cut(s) 1569, 3138
AjuI GAANNNNNNNTTGG 2 cut(s) 2334, 2366
Alw21I GWGCWC 3 cut(s) 1123, 2392, 2935
Alw26I GTCTC 7 cut(s) 187, 213, 1141, 1680, 1922, 2069, 3032
Alw44I GTGCAC 1 cut(s) 2388
AlwI GGATC 5 cut(s) 581, 1801, 2627, 2640, 2861
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 2445
AoxI GGCC 2 cut(s) 26, 522
ApaLI GTGCAC 1 cut(s) 2388
ApeKI GCWGC 6 cut(s) 1100, 2321, 2699, 3182, 3271, 3326
ApoI RAATTY 2 cut(s) 2330, 2893
ArsI GACNNNNNNTTYG 2 cut(s) 2713, 2745
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 2 cut(s) 2326, 2485
AspS9I GGNCC 7 cut(s) 63, 523, 976, 1271, 1445, 1809, 1823
AsuC2I CCSGG 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 3073
AvaI CYCGRG 1 cut(s) 2445
AvaII GGWCC 6 cut(s) 63, 976, 1271, 1445, 1809, 1823
AxyI CCTNAGG 3 cut(s) 693, 1521, 1776
BaeGI GKGCMC 1 cut(s) 2392
BaeI ACNNNNGTAYC 2 cut(s) 2514, 2547
BamHI GGATCC 1 cut(s) 2632
BauI CACGAG 1 cut(s) 2070
BbsI GAAGAC 2 cut(s) 269, 1203
Bbv12I GWGCWC 3 cut(s) 1123, 2392, 2935
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 6 cut(s) 1112, 2308, 2686, 3169, 3283, 3338
BceAI ACGGC 2 cut(s) 1445, 3004
BciT130I CCWGG 2 cut(s) 1571, 3140
BciVI GTATCC 2 cut(s) 2542, 2885
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 7 cut(s) 187, 213, 1141, 1680, 1922, 2069, 3032
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 3 cut(s) 252, 432, 2327
BfmI CTRYAG 5 cut(s) 1191, 1500, 1851, 2568, 2751
BfoI RGCGCY 1 cut(s) 2486
BfrI CTTAAG 1 cut(s) 2000
BfuAI ACCTGC 2 cut(s) 2255, 2830
BfuI GTATCC 2 cut(s) 2542, 2885
BisI GCNGC 7 cut(s) 29, 1101, 2322, 2700, 3183, 3272, 3327
BlsI GCNGC 7 cut(s) 30, 1102, 2323, 2701, 3184, 3273, 3328
Bme1390I CCNGG 3 cut(s) 242, 1571, 3140
Bme18I GGWCC 6 cut(s) 63, 976, 1271, 1445, 1809, 1823
BmeT110I CYCGRG 1 cut(s) 2445
BmgT120I GGNCC 7 cut(s) 63, 523, 976, 1271, 1445, 1809, 1823
BmiI GGNNCC 5 cut(s) 784, 1811, 1824, 2241, 2634
BmrFI CCNGG 3 cut(s) 242, 1571, 3140
BmrI ACTGGG 2 cut(s) 99, 291
BmsI GCATC 7 cut(s) 320, 928, 1888, 2281, 2590, 3169, 3258
BmuI ACTGGG 2 cut(s) 99, 291
BpiI GAAGAC 2 cut(s) 269, 1203
BpmI CTGGAG 2 cut(s) 1553, 2847
Bpu10I CCTNAGC 6 cut(s) 651, 752, 1267, 1287, 2135, 2547
Bpu14I TTCGAA 1 cut(s) 3073
BpuEI CTTGAG 3 cut(s) 649, 1025, 2492
BpuMI CCSGG 1 cut(s) 242
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 1 cut(s) 1476
BsaI GGTCTC 1 cut(s) 3032
BsaJI CCNNGG 7 cut(s) 240, 1261, 1608, 2703, 2823, 3138, 3139
BsaWI WCCGGW 1 cut(s) 3080
BsaXI ACNNNNNCTCC 2 cut(s) 2141, 2171
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 108, 461, 1819, 1988, 2249, 2492, 2756
Bse1I ACTGG 8 cut(s) 94, 168, 286, 315, 800, 862, 1385, 1982
Bse21I CCTNAGG 3 cut(s) 693, 1521, 1776
Bse3DI GCAATG 6 cut(s) 763, 1826, 2146, 2368, 2839, 3051
Bse8I GATNNNNATC 1 cut(s) 1476
BseBI CCWGG 2 cut(s) 1571, 3140
BseDI CCNNGG 7 cut(s) 240, 1261, 1608, 2703, 2823, 3138, 3139
BseGI GGATG 6 cut(s) 311, 772, 1333, 1378, 1903, 2353
BseJI GATNNNNATC 1 cut(s) 1476
BseLI CCNNNNNNNGG 8 cut(s) 89, 108, 461, 1819, 1988, 2249, 2492, 2756
BseMI GCAATG 6 cut(s) 763, 1826, 2146, 2368, 2839, 3051
BseNI ACTGG 8 cut(s) 94, 168, 286, 315, 800, 862, 1385, 1982
BseRI GAGGAG 5 cut(s) 205, 1022, 1589, 1594, 1607
BseSI GKGCMC 1 cut(s) 2392
BseXI GCAGC 6 cut(s) 1112, 2308, 2686, 3169, 3283, 3338
BseYI CCCAGC 1 cut(s) 326
BshFI GGCC 2 cut(s) 28, 524
BsiHKAI GWGCWC 3 cut(s) 1123, 2392, 2935
BsiHKCI CYCGRG 1 cut(s) 2445
BsiSI CCGG 3 cut(s) 242, 764, 3081
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 8 cut(s) 89, 108, 461, 1819, 1988, 2249, 2492, 2756
BsmAI GTCTC 7 cut(s) 187, 213, 1141, 1680, 1922, 2069, 3032
BsmBI CGTCTC 2 cut(s) 1141, 1922
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 1462
BsnI GGCC 2 cut(s) 28, 524
Bso31I GGTCTC 1 cut(s) 3032
BsoBI CYCGRG 1 cut(s) 2445
Bsp119I TTCGAA 1 cut(s) 3073
Bsp1286I GDGCHC 3 cut(s) 1123, 2392, 2935
Bsp1407I TGTACA 2 cut(s) 13, 2077
Bsp143I GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
BspACI CCGC 8 cut(s) 29, 174, 213, 1091, 1892, 2579, 3024, 3219
BspANI GGCC 2 cut(s) 28, 524
BspHI TCATGA 1 cut(s) 1486
BspLI GGNNCC 5 cut(s) 784, 1811, 1824, 2241, 2634
BspMAI CTGCAG 1 cut(s) 2572
BspMI ACCTGC 2 cut(s) 2255, 2830
BspPI GGATC 5 cut(s) 581, 1801, 2627, 2640, 2861
BspQI GCTCTTC 1 cut(s) 1065
BspT104I TTCGAA 1 cut(s) 3073
BspTI CTTAAG 1 cut(s) 2000
BspTNI GGTCTC 1 cut(s) 3032
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 6 cut(s) 763, 1826, 2146, 2368, 2839, 3051
BsrGI TGTACA 2 cut(s) 13, 2077
BsrI ACTGG 8 cut(s) 94, 168, 286, 315, 800, 862, 1385, 1982
BssECI CCNNGG 7 cut(s) 240, 1261, 1608, 2703, 2823, 3138, 3139
BssMI GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
BssNAI GTATAC 1 cut(s) 1693
BssSI CACGAG 1 cut(s) 2070
BssT1I CCWWGG 3 cut(s) 1608, 2703, 2823
Bst1107I GTATAC 1 cut(s) 1693
Bst2BI CACGAG 1 cut(s) 2070
Bst2UI CCWGG 2 cut(s) 1571, 3140
Bst6I CTCTTC 3 cut(s) 364, 390, 1065
BstAFI CTTAAG 1 cut(s) 2000
BstAPI GCANNNNNTGC 2 cut(s) 1604, 2396
BstAUI TGTACA 2 cut(s) 13, 2077
BstBAI YACGTR 1 cut(s) 1604
BstBI TTCGAA 1 cut(s) 3073
BstC8I GCNNGC 8 cut(s) 324, 467, 648, 1047, 1065, 2808, 2935, 3297
BstENI CCTNNNNNAGG 1 cut(s) 2754
BstF5I GGATG 6 cut(s) 311, 772, 1333, 1378, 1903, 2353
BstH2I RGCGCY 1 cut(s) 2486
BstHHI GCGC 2 cut(s) 2326, 2485
BstKTI GATC 9 cut(s) 589, 712, 1140, 1796, 2416, 2635, 2746, 2869, 2929
BstMAI GTCTC 7 cut(s) 187, 213, 1141, 1680, 1922, 2069, 3032
BstMBI GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
BstNI CCWGG 2 cut(s) 1571, 3140
BstNSI RCATGY 2 cut(s) 2425, 2810
BstSCI CCNGG 3 cut(s) 240, 1569, 3138
BstSFI CTRYAG 5 cut(s) 1191, 1500, 1851, 2568, 2751
BstSLI GKGCMC 1 cut(s) 2392
BstV1I GCAGC 6 cut(s) 1112, 2308, 2686, 3169, 3283, 3338
BstV2I GAAGAC 2 cut(s) 269, 1203
BstX2I RGATCY 3 cut(s) 586, 2632, 2866
BstYI RGATCY 3 cut(s) 586, 2632, 2866
BstZ17I GTATAC 1 cut(s) 1693
Bsu36I CCTNAGG 3 cut(s) 693, 1521, 1776
BsuI GTATCC 2 cut(s) 2542, 2885
BsuRI GGCC 2 cut(s) 28, 524
BtsCI GGATG 6 cut(s) 311, 772, 1333, 1378, 1903, 2353
BtsIMutI CAGTG 5 cut(s) 322, 1695, 1878, 1989, 2960
BveI ACCTGC 2 cut(s) 2255, 2830
Cac8I GCNNGC 8 cut(s) 324, 467, 648, 1047, 1065, 2808, 2935, 3297
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 1486
CfoI GCGC 2 cut(s) 2326, 2485
Cfr13I GGNCC 7 cut(s) 63, 523, 976, 1271, 1445, 1809, 1823
CseI GACGC 1 cut(s) 244
Csp6I GTAC 5 cut(s) 14, 1207, 1403, 2078, 2820
CviQI GTAC 5 cut(s) 14, 1207, 1403, 2078, 2820
DpnI GATC 9 cut(s) 588, 711, 1139, 1795, 2415, 2634, 2745, 2868, 2928
DpnII GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
DraI TTTAAA 3 cut(s) 594, 1228, 2410
Eam1104I CTCTTC 3 cut(s) 364, 390, 1065
EarI CTCTTC 3 cut(s) 364, 390, 1065
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 1608, 2703, 2823
Eco31I GGTCTC 1 cut(s) 3032
Eco32I GATATC 1 cut(s) 2362
Eco47I GGWCC 6 cut(s) 63, 976, 1271, 1445, 1809, 1823
Eco57I CTGAAG 3 cut(s) 375, 441, 802
Eco81I CCTNAGG 3 cut(s) 693, 1521, 1776
Eco88I CYCGRG 1 cut(s) 2445
EcoNI CCTNNNNNAGG 1 cut(s) 2754
EcoO109I RGGNCCY 2 cut(s) 523, 1271
EcoRII CCWGG 2 cut(s) 1569, 3138
EcoRV GATATC 1 cut(s) 2362
EcoT14I CCWWGG 3 cut(s) 1608, 2703, 2823
EcoT22I ATGCAT 2 cut(s) 1903, 2427
ErhI CCWWGG 3 cut(s) 1608, 2703, 2823
Esp3I CGTCTC 2 cut(s) 1141, 1922
FalI AAGNNNNNCTT 8 cut(s) 100, 132, 714, 746, 1017, 1049, 2696, 2728
FaqI GGGAC 1 cut(s) 299
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 709
FblI GTMKAC 2 cut(s) 1692, 2229
Fnu4HI GCNGC 7 cut(s) 29, 1101, 2322, 2700, 3183, 3272, 3327
FokI GGATG 6 cut(s) 298, 779, 1340, 1385, 1910, 2340
Fsp4HI GCNGC 7 cut(s) 29, 1101, 2322, 2700, 3183, 3272, 3327
FspBI CTAG 3 cut(s) 252, 432, 2327
GlaI GCGC 2 cut(s) 2325, 2484
GluI GCNGC 7 cut(s) 29, 1101, 2322, 2700, 3183, 3272, 3327
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 2 cut(s) 1553, 2847
HaeII RGCGCY 1 cut(s) 2486
HaeIII GGCC 2 cut(s) 28, 524
HapII CCGG 3 cut(s) 242, 764, 3081
HgaI GACGC 1 cut(s) 244
HhaI GCGC 2 cut(s) 2326, 2485
Hin6I GCGC 2 cut(s) 2324, 2483
HinP1I GCGC 2 cut(s) 2324, 2483
HincII GTYRAC 5 cut(s) 361, 409, 576, 2101, 3112
HindII GTYRAC 5 cut(s) 361, 409, 576, 2101, 3112
HinfI GANTC 8 cut(s) 268, 449, 881, 1300, 2006, 2062, 2253, 2790
HpaI GTTAAC 1 cut(s) 576
HpaII CCGG 3 cut(s) 242, 764, 3081
Hpy188I TCNGA 9 cut(s) 394, 541, 1363, 1680, 1764, 1927, 2515, 2674, 2733
HpyCH4IV ACGT 3 cut(s) 1200, 1603, 2097
HpySE526I ACGT 3 cut(s) 1200, 1603, 2097
HspAI GCGC 2 cut(s) 2324, 2483
Ksp22I TGATCA 1 cut(s) 709
KspAI GTTAAC 1 cut(s) 576
Kzo9I GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
LguI GCTCTTC 1 cut(s) 1065
LmnI GCTCC 9 cut(s) 782, 1042, 1572, 2239, 2480, 2581, 2866, 3173, 3313
Lsp1109I GCAGC 6 cut(s) 1112, 2308, 2686, 3169, 3283, 3338
LweI GCATC 7 cut(s) 320, 928, 1888, 2281, 2590, 3169, 3258
MaeI CTAG 3 cut(s) 252, 432, 2327
MaeII ACGT 3 cut(s) 1200, 1603, 2097
MaeIII GTNAC 7 cut(s) 1376, 1721, 1729, 1845, 2007, 2056, 2907
MalI GATC 9 cut(s) 588, 711, 1139, 1795, 2415, 2634, 2745, 2868, 2928
MbiI CCGCTC 1 cut(s) 31
MboI GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
MfeI CAATTG 2 cut(s) 477, 1338
MflI RGATCY 3 cut(s) 586, 2632, 2866
MhlI GDGCHC 3 cut(s) 1123, 2392, 2935
MlyI GAGTC 3 cut(s) 890, 2015, 2071
MmeI TCCRAC 8 cut(s) 76, 103, 585, 782, 1301, 1787, 2017, 2559
Mph1103I ATGCAT 2 cut(s) 1903, 2427
MroXI GAANNNNTTC 1 cut(s) 112
MslI CAYNNNNRTG 6 cut(s) 722, 959, 1485, 1982, 2373, 3044
MspA1I CMGCKG 1 cut(s) 1894
MspCI CTTAAG 1 cut(s) 2000
MspI CCGG 3 cut(s) 242, 764, 3081
MspR9I CCNGG 3 cut(s) 242, 1571, 3140
MunI CAATTG 2 cut(s) 477, 1338
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 2 cut(s) 1571, 3140
NciI CCSGG 1 cut(s) 242
NdeII GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
NlaIV GGNNCC 5 cut(s) 784, 1811, 1824, 2241, 2634
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 3 cut(s) 1376, 1729, 2007
NsiI ATGCAT 2 cut(s) 1903, 2427
NspI RCATGY 2 cut(s) 2425, 2810
NspV TTCGAA 1 cut(s) 3073
PaeI GCATGC 1 cut(s) 2810
PagI TCATGA 1 cut(s) 1486
PasI CCCWGGG 1 cut(s) 3139
PciSI GCTCTTC 1 cut(s) 1065
PctI GAATGC 1 cut(s) 1462
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 5 cut(s) 268, 449, 1300, 2253, 2790
PflMI CCANNNNNTGG 1 cut(s) 1988
PfoI TCCNGGA 1 cut(s) 1569
PkrI GCNGC 7 cut(s) 30, 1102, 2323, 2701, 3184, 3273, 3328
PleI GAGTC 3 cut(s) 889, 2014, 2070
PpsI GAGTC 3 cut(s) 889, 2014, 2070
Ppu21I YACGTR 1 cut(s) 1604
PpuMI RGGWCCY 1 cut(s) 1271
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 2 cut(s) 1569, 3138
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 1569, 3138
PspN4I GGNNCC 5 cut(s) 784, 1811, 1824, 2241, 2634
PspPI GGNCC 7 cut(s) 63, 523, 976, 1271, 1445, 1809, 1823
PspPPI RGGWCCY 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 2572
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 3 cut(s) 586, 2632, 2866
RsaI GTAC 5 cut(s) 15, 1208, 1404, 2079, 2821
RsaNI GTAC 5 cut(s) 14, 1207, 1403, 2078, 2820
RseI CAYNNNNRTG 6 cut(s) 722, 959, 1485, 1982, 2373, 3044
SapI GCTCTTC 1 cut(s) 1065
SatI GCNGC 7 cut(s) 29, 1101, 2322, 2700, 3183, 3272, 3327
Sau3AI GATC 9 cut(s) 586, 709, 1137, 1793, 2413, 2632, 2743, 2866, 2926
Sau96I GGNCC 7 cut(s) 63, 523, 976, 1271, 1445, 1809, 1823
SchI GAGTC 3 cut(s) 890, 2015, 2071
ScrFI CCNGG 3 cut(s) 242, 1571, 3140
SduI GDGCHC 3 cut(s) 1123, 2392, 2935
SfaNI GCATC 7 cut(s) 320, 928, 1888, 2281, 2590, 3169, 3258
SfcI CTRYAG 5 cut(s) 1191, 1500, 1851, 2568, 2751
SfuI TTCGAA 1 cut(s) 3073
SinI GGWCC 6 cut(s) 63, 976, 1271, 1445, 1809, 1823
SmiMI CAYNNNNRTG 6 cut(s) 722, 959, 1485, 1982, 2373, 3044
SmlI CTYRAG 4 cut(s) 664, 1004, 2000, 2471
SmoI CTYRAG 4 cut(s) 664, 1004, 2000, 2471
SpeI ACTAGT 1 cut(s) 431
SphI GCATGC 1 cut(s) 2810
SsiI CCGC 8 cut(s) 29, 174, 213, 1091, 1892, 2579, 3024, 3219
SspI AATATT 1 cut(s) 681
SspMI CTAG 3 cut(s) 252, 432, 2327
StyD4I CCNGG 3 cut(s) 240, 1569, 3138
StyI CCWWGG 3 cut(s) 1608, 2703, 2823
TaiI ACGT 3 cut(s) 1203, 1606, 2100
TaqI TCGA 7 cut(s) 106, 353, 444, 493, 3073, 3147, 3303
TatI WGTACW 2 cut(s) 13, 2077
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 5 cut(s) 268, 449, 1300, 2253, 2790
TscAI CASTG 5 cut(s) 322, 1702, 1885, 1989, 2967
TseFI GTSAC 3 cut(s) 1376, 1729, 2007
TseI GCWGC 6 cut(s) 1100, 2321, 2699, 3182, 3271, 3326
Tsp45I GTSAC 3 cut(s) 1376, 1729, 2007
TspGWI ACGGA 1 cut(s) 1946
TspRI CASTG 5 cut(s) 322, 1702, 1885, 1989, 2967
Van91I CCANNNNNTGG 1 cut(s) 1988
Vha464I CTTAAG 1 cut(s) 2000
VneI GTGCAC 1 cut(s) 2388
VpaK11BI GGWCC 6 cut(s) 63, 976, 1271, 1445, 1809, 1823
XagI CCTNNNNNAGG 1 cut(s) 2754
XapI RAATTY 2 cut(s) 2330, 2893
XceI RCATGY 2 cut(s) 2425, 2810
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 1692, 2229
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 3 cut(s) 252, 432, 2327
Zsp2I ATGCAT 2 cut(s) 1903, 2427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.