RLG00000031072

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003232
Physical Location & Seq
Reverse (-)
1766875 .. 1774029
7155 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031072

Sequence Viewer

Length: 3279 bp
ATGGACGTGTCTTTGCAAGCTCATAGGCCACTCAGTTGCGGAACAGTGTTTCAAGACAGAAGAAGCATTTTGAAGCTCAAACCACTTACTGGGTTTTGTTCTAATGGGAGATATTCTTCATGGTTCAAAGGAGACCTGACAAGTGGGGCTTCATGTAAAATTACTGCTAGTTCAGCTGATTTCTCTAGGAGGAGACAGAAAAAAGTGTCAGGTTCAAGGCCTAATGGCCCTGGTCCAAAGGCAATAGTTCCCAAAACCCCAATAGTAACTAGCATCCCGAAGACAAATCAGAGGAATACTGGGGATAAAAAGGGTTTTACGTCTTCTACAGCCAGTGTTGTTGGGCCCAAAGTTGATGTAAAAAGGATTGATGAAACTAGTCGTAAAGTTGAGGAGTCTTCGTCACGTAGCAAAACATCACCTACTGGTAGAAGCTTTCAAGAGGTAGAAGATGGGAGTATAGATAAAGGCATTGATATTGATAAGAAGTTATCTGATGAAGCTTCTTTGAAACTAAAGTTGGAGATGGAAGAAAAACAACGTATAGAGGAGATTGAGAGGTTAGCTGAGGAAAACTTCTCAAGAGGGAAGAAGCTCTTTGTCTATCCTCAAGTGGTGAAACCTGATCAACATATAGAAGTGTTTCTTAATAAAAGTCTTTCCACCCTAAACAGTGAAGATGATGTTTTCATCATGGGAGCCTTTAATGAATGGCGCTGGAAATCTTTTACCTTTCGGTTGAGCAGAACAAATCTCAAAGGGGATTGGTGGTCTTGTCAATTTCATGTTCCTGCCGAAGCATATAAGATAGACTTTGTGTTCTTCAATGGAAAAGATGTTTATGACAATAATGATAAGAAGGATTTCTGCATAGATGTAGAAAGTGGAATGGACGCCTTTGCATTTGAAGATTTCTTACTTGAGGAGAAGCGTAAAGAACTCGAGAAACTTGCAGAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAAGCGAATTGAAGCCGAGAAAGCAGCACAAGAAGCTGACAGAGCAGAGGCAAAGGGAGAGACTGAAAGGAGACGAAAATTGGTGCAGGAGTTGACCAAAAAGGCTGTAAGGTCTGTTCCGGATGTTTGGTACATAGAGCCTAGTGAGTTTAAAGGTAAGGACTTGGTGAGGTTATATTATAACCGAAGCTCAGGTCCTCTTGCTGGTGCTAATGAATTATGGGTTCATGGGGGACATAATGGTTGGAGTTCTGGCTTATCAATTGTTGAAAGTCTTGTTAGATCTGAGGAAAAGGATGGTGACTGGTGGTATGCCAAAGTTGTTGTACCTGACCAAGCTATGGTATTGGATTGGGTTTTTGCTGATGGACCGCCTCAGAATGCCACTGTATATGATAACAATCATCGTCATGATTTCCATGCAGTAGTTCCAAAGTCCATTCCTCAGGAACAATATTGGGTTGAGGAAGAACACCAGATCTATAGGAAGCTCCAGGAGGAGCGGAGATTAAGGGAGGAGGCCATACGTGCCAAGGCCAAAAAAACAGCACTTATGAAAGCAAAAATGAAGGAACGAACTCTGAGAAGGTATCTGCTGTCTCAGAAGCATATAGTCTACACTGAGCCTCTTGAGGTCCAAGCCGGAAGCATGGTAACGATTTTCTACAATCCTGCCAATACTGCTTTGCACGGAAAACCTGAGGTCTGGTTCAGATGCTCCTTTAACCATTGGGCCCACCGCAGTGGTCCCTTGCCACCACAGAAAATGGTACCTGCAGAGAATGGTACTCATGTCAAAACCACTGTCATGGTTCCGCTCGATGCATATGTGATGGACTTTGTCTTCTCTGAGAGTGAAGAAGGTGGACTTTTTGACAACAAAAATGACATGGATTATCACATCCCAGTGTTTGGAGGAGTTTTGAAGGAGTCACCAATGCACATTGTGCATATTACGGTTGAAATGGCACCCATTGCAAAGGTTGGAGGCCTTGGTGATGTGGTTACCAGTCTATCCCGAGCTGTGCAAGACTTAAATCACCATGTCGACGTCATTCTTCCCAAGTATGACTGTTTGAACCTTAGCAATGTTAAGGAATTTCAGTATAAAAGAAGCTATTCTTGGGGTGGTACTGAAATCAAAGTTTGGTTTGGGAAAGTGGAAGGAGTTTCAGTCTACTTTTTGGAACCTCAAAATGGACTGTTTTATGCGGGTTGTATATATGGTTGTAAGAATGATGCGGAGAGATTTGGCTTCTTTTGCCATGCTGCTCTAGAATATCTACTCCAAAGTGGATCCCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCGCCTGTTGCCTGGTTATATAAAGATCATTACGTGCATTATGGTCTTAGTAAAGCTCGCATTGTCTTCACAATTCATAATCTTGAATTTGGAGCACACTTTATTGGAAAAGCTGTGGCATACTCAGACAAGTCCACTACTGTATCCCACACTTACTCTAAGGAGATTGCTGGAAATCCTGCAGTAGCTCCATACCTCTACAAGTTCCATGGCATAATAAATGGAATTGACCAAGATATATGGGACCCATACAATGATGGATTCCTACCTATATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGCAGATCTCCCTGTAGTAGGTATTATTACTCGATTAACTCAGCAGAAAGGAATCCATCTTATCAAGCATGCCATTCGGCGTACCTTGGAACGCAATGGACAGGTTGTATTGCTTGGTTCAGCTCCAGATCCTCGTATCCAAAATGAGTTTGTAAATTTGGCCAATCAATTACATTCTTCTCATGGTGATCGTGCTCGTCTTTGTTTGACTTATGATGAGCCACTCTCACATTTGATATACGCTGGTGCTGATTTCATCCTAGTTCCTTCAATATTTGAGCCATGTGGGCTGACCCAACTTGTAGCAATGAGATACGGTTCCATACCTGTTGTTCGAAAAACCGGAGGGCTCTACGACACTGTATTTGATGTTGACCATGATAAAGAGAGAGCAGAGGCGCAGGGTCTTGAACCAAATGGGTTCAGTTTTGACGGAGCTGATGCTGCTGGTGTTGATTATGCTCTAAATAGAGCTATCTCTGCCTGGTATGATGGTCGGGATTGGTTCAACTCGTTGTGCAAAACAGTGATGGAGCAAGATTGGTCTTGGAACCGACCAGCTCTCGATTATATGGAGCTGTATCACGCAGCACGGAAGTGA

Protein Analysis

1093

Amino Acids

123.91

Weight (kDa)

6.56

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 213 - 272 2.9e-11 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 387 - 449 2.1e-13 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 549 - 611 2.4e-10 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 645 - 833 4.9e-48 Starch synthase catalytic domain
Glyco_transf_4 PF13439 659 - 831 2.9e-08 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 894 - 1014 3.5e-06 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 897 - 1014 3.6e-06 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1173
AatII GACGTC 1 cut(s) 2046
Acc36I ACCTGC 1 cut(s) 1774
Acc65I GGTACC 1 cut(s) 1762
AccB1I GGYRCC 2 cut(s) 1762, 1960
AccB7I CCANNNNNTGG 3 cut(s) 89, 1333, 1904
AccBSI CCGCTC 2 cut(s) 1495, 1810
AccI GTMKAC 3 cut(s) 1608, 2040, 2169
AccIII TCCGGA 1 cut(s) 1111
AciI CCGC 7 cut(s) 39, 1364, 1495, 1732, 1808, 2204, 2234
AclWI GGATC 3 cut(s) 2283, 2296, 2801
AcoI YGGCCR 1 cut(s) 2838
AcsI RAATTY 3 cut(s) 2090, 2414, 2833
AcyI GRCGYC 2 cut(s) 894, 2043
AdeI CACNNNGTG 1 cut(s) 1939
AfaI GTAC 6 cut(s) 1124, 1320, 1764, 1780, 2125, 2761
AfiI CCNNNNNNNGG 6 cut(s) 89, 1196, 1333, 1904, 2189, 2696
AflIII ACRYGT 1 cut(s) 6
AhlI ACTAGT 1 cut(s) 377
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 4 cut(s) 229, 1485, 2339, 3161
AjuI GAANNNNNNNTTGG 4 cut(s) 503, 535, 2098, 2130
AleI CACNNNNGTG 2 cut(s) 1734, 3274
Alw21I GWGCWC 2 cut(s) 2425, 2875
Alw26I GTCTC 5 cut(s) 126, 187, 1046, 1057, 1596
AlwI GGATC 3 cut(s) 2283, 2296, 2801
Ama87I CYCGRG 2 cut(s) 941, 2010
Aor13HI TCCGGA 1 cut(s) 1111
AoxI GGCC 9 cut(s) 26, 218, 226, 344, 1512, 1527, 1725, 1981, 2838
ApaI GGGCCC 2 cut(s) 348, 1729
ApeKI GCWGC 5 cut(s) 1016, 2261, 2639, 3122, 3266
ApoI RAATTY 3 cut(s) 2090, 2414, 2833
ArsI GACNNNNNNTTYG 8 cut(s) 1171, 1203, 1782, 1814, 2653, 2685, 3026, 3058
Asp700I GAANNNNTTC 3 cut(s) 642, 863, 2110
Asp718I GGTACC 1 cut(s) 1762
AspLEI GCGC 3 cut(s) 717, 2332, 3079
AsuHPI GGTGA 8 cut(s) 411, 628, 1171, 1304, 1917, 2000, 2024, 2876
AsuII TTCGAA 1 cut(s) 3013
AvaI CYCGRG 2 cut(s) 941, 2010
AvaII GGWCC 6 cut(s) 233, 1187, 1361, 1627, 1739, 2572
AxyI CCTNAGG 2 cut(s) 1437, 1692
BaeGI GKGCMC 2 cut(s) 348, 1729
BaeI ACNNNNGTAYC 4 cut(s) 1770, 1803, 2454, 2487
BalI TGGCCA 1 cut(s) 2840
BamHI GGATCC 1 cut(s) 2288
BanI GGYRCC 2 cut(s) 1762, 1960
BanII GRGCYC 3 cut(s) 348, 1729, 3030
BarI GAAGNNNNNNTAC 2 cut(s) 900, 932
BbsI GAAGAC 5 cut(s) 287, 315, 390, 1828, 2386
Bbv12I GWGCWC 2 cut(s) 2425, 2875
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 4 cut(s) 1028, 2248, 2626, 3109
BcgI CGANNNNNNTGC 4 cut(s) 932, 966, 2735, 2769
BciT130I CCWGG 4 cut(s) 231, 1487, 2341, 3163
BciVI GTATCC 2 cut(s) 2482, 2825
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 5 cut(s) 126, 187, 1046, 1057, 1596
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 8 cut(s) 168, 186, 270, 378, 1134, 2267, 2325, 2939
BfmI CTRYAG 5 cut(s) 327, 1474, 1767, 2508, 2691
BfoI RGCGCY 1 cut(s) 718
BfuAI ACCTGC 1 cut(s) 1774
BfuI GTATCC 2 cut(s) 2482, 2825
BglI GCCNNNNNGGC 1 cut(s) 2965
BglII AGATCT 3 cut(s) 1274, 1470, 2683
BisI GCNGC 5 cut(s) 1017, 2262, 2640, 3123, 3267
BlsI GCNGC 5 cut(s) 1018, 2263, 2641, 3124, 3268
Bme1390I CCNGG 4 cut(s) 231, 1487, 2341, 3163
Bme18I GGWCC 6 cut(s) 233, 1187, 1361, 1627, 1739, 2572
BmeT110I CYCGRG 2 cut(s) 941, 2010
BmgBI CACGTC 1 cut(s) 7
BmrFI CCNGG 4 cut(s) 231, 1487, 2341, 3163
BmrI ACTGGG 3 cut(s) 99, 309, 1892
BmsI GCATC 5 cut(s) 282, 1697, 1804, 2221, 3109
BmuI ACTGGG 3 cut(s) 99, 309, 1892
BpiI GAAGAC 5 cut(s) 287, 315, 390, 1828, 2386
BplI GAGNNNNNCTC 2 cut(s) 2888, 2920
BpmI CTGGAG 2 cut(s) 1469, 2787
Bpu10I CCTNAGC 3 cut(s) 567, 1183, 2075
Bpu14I TTCGAA 1 cut(s) 3013
BpuEI CTTGAG 4 cut(s) 565, 594, 941, 1643
BsaAI YACGTR 3 cut(s) 407, 1520, 2362
BsaBI GATNNNNATC 1 cut(s) 1392
BsaHI GRCGYC 2 cut(s) 894, 2043
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 6 cut(s) 229, 1524, 1984, 2536, 2643, 2763
BsaWI WCCGGW 2 cut(s) 1111, 3020
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 2151, 2181
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 1196, 1333, 1904, 2189, 2696
Bse1I ACTGG 7 cut(s) 94, 304, 333, 430, 1301, 1898, 2001
Bse21I CCTNAGG 2 cut(s) 1437, 1692
Bse3DI GCAATG 5 cut(s) 1965, 2086, 2308, 2779, 2991
Bse8I GATNNNNATC 1 cut(s) 1392
BseAI TCCGGA 1 cut(s) 1111
BseBI CCWGG 4 cut(s) 231, 1487, 2341, 3163
BseDI CCNNGG 6 cut(s) 229, 1524, 1984, 2536, 2643, 2763
BseGI GGATG 6 cut(s) 273, 1120, 1294, 1893, 2293, 2934
BseJI GATNNNNATC 1 cut(s) 1392
BseLI CCNNNNNNNGG 6 cut(s) 89, 1196, 1333, 1904, 2189, 2696
BseMI GCAATG 5 cut(s) 1965, 2086, 2308, 2779, 2991
BseNI ACTGG 7 cut(s) 94, 304, 333, 430, 1301, 1898, 2001
BseRI GAGGAG 8 cut(s) 205, 407, 563, 938, 971, 1505, 1523, 1923
BseSI GKGCMC 2 cut(s) 348, 1729
BseXI GCAGC 4 cut(s) 1028, 2248, 2626, 3109
BsgI GTGCAG 1 cut(s) 1097
BshFI GGCC 9 cut(s) 28, 220, 228, 346, 1514, 1529, 1727, 1983, 2840
BshNI GGYRCC 2 cut(s) 1762, 1960
BsiHKAI GWGCWC 2 cut(s) 2425, 2875
BsiHKCI CYCGRG 2 cut(s) 941, 2010
BsiSI CCGG 3 cut(s) 1112, 1635, 3021
BslFI GGGAC 3 cut(s) 1239, 1725, 2585
BslI CCNNNNNNNGG 6 cut(s) 89, 1196, 1333, 1904, 2189, 2696
BsmAI GTCTC 5 cut(s) 126, 187, 1046, 1057, 1596
BsmBI CGTCTC 1 cut(s) 1057
BsmFI GGGAC 3 cut(s) 1239, 1725, 2585
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 9 cut(s) 28, 220, 228, 346, 1514, 1529, 1727, 1983, 2840
Bso31I GGTCTC 1 cut(s) 126
BsoBI CYCGRG 2 cut(s) 941, 2010
Bsp119I TTCGAA 1 cut(s) 3013
Bsp120I GGGCCC 2 cut(s) 344, 1725
Bsp1286I GDGCHC 5 cut(s) 348, 1729, 2425, 2875, 3030
Bsp13I TCCGGA 1 cut(s) 1111
Bsp143I GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
Bsp19I CCATGG 1 cut(s) 2536
BspACI CCGC 7 cut(s) 39, 1364, 1495, 1732, 1808, 2204, 2234
BspANI GGCC 9 cut(s) 28, 220, 228, 346, 1514, 1529, 1727, 1983, 2840
BspEI TCCGGA 1 cut(s) 1111
BspHI TCATGA 1 cut(s) 1402
BspMAI CTGCAG 2 cut(s) 1771, 2512
BspMI ACCTGC 1 cut(s) 1774
BspPI GGATC 3 cut(s) 2283, 2296, 2801
BspQI GCTCTTC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 3013
BspT107I GGYRCC 2 cut(s) 1762, 1960
BspTNI GGTCTC 1 cut(s) 126
BsrBI CCGCTC 2 cut(s) 1495, 1810
BsrDI GCAATG 5 cut(s) 1965, 2086, 2308, 2779, 2991
BsrI ACTGG 7 cut(s) 94, 304, 333, 430, 1301, 1898, 2001
BssECI CCNNGG 6 cut(s) 229, 1524, 1984, 2536, 2643, 2763
BssMI GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
BssNI GRCGYC 2 cut(s) 894, 2043
BssT1I CCWWGG 5 cut(s) 1524, 1984, 2536, 2643, 2763
Bst2UI CCWGG 4 cut(s) 231, 1487, 2341, 3163
Bst6I CTCTTC 1 cut(s) 981
BstACI GRCGYC 2 cut(s) 894, 2043
BstAPI GCANNNNNTGC 2 cut(s) 1939, 1967
BstBAI YACGTR 3 cut(s) 407, 1520, 2362
BstBI TTCGAA 1 cut(s) 3013
BstC8I GCNNGC 5 cut(s) 18, 963, 981, 2386, 2748
BstDSI CCRYGG 1 cut(s) 2536
BstEII GGTNACC 1 cut(s) 1996
BstENI CCTNNNNNAGG 1 cut(s) 2694
BstF5I GGATG 6 cut(s) 273, 1120, 1294, 1893, 2293, 2934
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 3 cut(s) 717, 2332, 3079
BstKTI GATC 8 cut(s) 628, 1277, 1473, 2291, 2356, 2686, 2809, 2869
BstMAI GTCTC 5 cut(s) 126, 187, 1046, 1057, 1596
BstMBI GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
BstNI CCWGG 4 cut(s) 231, 1487, 2341, 3163
BstNSI RCATGY 1 cut(s) 2750
BstPI GGTNACC 1 cut(s) 1996
BstSCI CCNGG 4 cut(s) 229, 1485, 2339, 3161
BstSFI CTRYAG 5 cut(s) 327, 1474, 1767, 2508, 2691
BstSLI GKGCMC 2 cut(s) 348, 1729
BstV1I GCAGC 4 cut(s) 1028, 2248, 2626, 3109
BstV2I GAAGAC 5 cut(s) 287, 315, 390, 1828, 2386
BstX2I RGATCY 5 cut(s) 1274, 1470, 2288, 2683, 2806
BstXI CCANNNNNNTGG 2 cut(s) 1736, 1801
BstYI RGATCY 5 cut(s) 1274, 1470, 2288, 2683, 2806
Bsu36I CCTNAGG 2 cut(s) 1437, 1692
BsuI GTATCC 2 cut(s) 2482, 2825
BsuRI GGCC 9 cut(s) 28, 220, 228, 346, 1514, 1529, 1727, 1983, 2840
BtgI CCRYGG 1 cut(s) 2536
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 6 cut(s) 273, 1120, 1294, 1893, 2293, 2934
BtsI GCAGTG 1 cut(s) 1741
BveI ACCTGC 1 cut(s) 1774
Cac8I GCNNGC 5 cut(s) 18, 963, 981, 2386, 2748
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 3 cut(s) 717, 2332, 3079
CseI GACGC 1 cut(s) 902
Csp6I GTAC 6 cut(s) 1123, 1319, 1763, 1779, 2124, 2760
CviQI GTAC 6 cut(s) 1123, 1319, 1763, 1779, 2124, 2760
DpnI GATC 8 cut(s) 627, 1276, 1472, 2290, 2355, 2685, 2808, 2868
DpnII GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
DraI TTTAAA 1 cut(s) 1144
DraIII CACNNNGTG 1 cut(s) 1939
EaeI YGGCCR 1 cut(s) 2838
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
Eco130I CCWWGG 5 cut(s) 1524, 1984, 2536, 2643, 2763
Eco147I AGGCCT 2 cut(s) 220, 1983
Eco24I GRGCYC 3 cut(s) 348, 1729, 3030
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 1 cut(s) 2302
Eco47I GGWCC 6 cut(s) 233, 1187, 1361, 1627, 1739, 2572
Eco81I CCTNAGG 2 cut(s) 1437, 1692
Eco88I CYCGRG 2 cut(s) 941, 2010
Eco91I GGTNACC 1 cut(s) 1996
EcoNI CCTNNNNNAGG 1 cut(s) 2694
EcoO109I RGGNCCY 2 cut(s) 1187, 2572
EcoO65I GGTNACC 1 cut(s) 1996
EcoRII CCWGG 4 cut(s) 229, 1485, 2339, 3161
EcoRV GATATC 1 cut(s) 2302
EcoT14I CCWWGG 5 cut(s) 1524, 1984, 2536, 2643, 2763
EcoT22I ATGCAT 1 cut(s) 1819
EcoT38I GRGCYC 3 cut(s) 348, 1729, 3030
ErhI CCWWGG 5 cut(s) 1524, 1984, 2536, 2643, 2763
Esp3I CGTCTC 1 cut(s) 1057
FaqI GGGAC 3 cut(s) 1239, 1725, 2585
FauI CCCGC 1 cut(s) 2197
FauNDI CATATG 1 cut(s) 1819
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 3 cut(s) 1608, 2040, 2169
Fnu4HI GCNGC 5 cut(s) 1017, 2262, 2640, 3123, 3267
FokI GGATG 6 cut(s) 260, 1127, 1301, 1880, 2280, 2921
FriOI GRGCYC 3 cut(s) 348, 1729, 3030
Fsp4HI GCNGC 5 cut(s) 1017, 2262, 2640, 3123, 3267
FspBI CTAG 8 cut(s) 168, 186, 270, 378, 1134, 2267, 2325, 2939
GlaI GCGC 3 cut(s) 716, 2331, 3078
GluI GCNGC 5 cut(s) 1017, 2262, 2640, 3123, 3267
GsuI CTGGAG 2 cut(s) 1469, 2787
HaeII RGCGCY 1 cut(s) 718
HaeIII GGCC 9 cut(s) 28, 220, 228, 346, 1514, 1529, 1727, 1983, 2840
HapII CCGG 3 cut(s) 1112, 1635, 3021
HgaI GACGC 1 cut(s) 902
HhaI GCGC 3 cut(s) 717, 2332, 3079
Hin1I GRCGYC 2 cut(s) 894, 2043
Hin6I GCGC 3 cut(s) 715, 2330, 3077
HinP1I GCGC 3 cut(s) 715, 2330, 3077
HincII GTYRAC 3 cut(s) 1086, 2041, 3052
HindII GTYRAC 3 cut(s) 1086, 2041, 3052
HindIII AAGCTT 2 cut(s) 433, 501
HinfI GANTC 4 cut(s) 395, 1922, 2589, 2730
HpaII CCGG 3 cut(s) 1112, 1635, 3021
HphI GGTGA 8 cut(s) 411, 628, 1171, 1304, 1917, 2000, 2024, 2876
Hpy166II GTNNAC 7 cut(s) 1086, 1609, 1859, 2041, 2170, 2463, 3052
Hpy8I GTNNAC 7 cut(s) 1086, 1609, 1859, 2041, 2170, 2463, 3052
Hpy99I CGWCG 1 cut(s) 2045
HpyAV CCTTC 8 cut(s) 853, 1555, 1572, 1847, 1912, 2150, 2621, 2955
HpyCH4IV ACGT 7 cut(s) 6, 320, 406, 541, 1519, 2043, 2361
HpySE526I ACGT 7 cut(s) 6, 320, 406, 541, 1519, 2043, 2361
Hsp92I GRCGYC 2 cut(s) 894, 2043
HspAI GCGC 3 cut(s) 715, 2330, 3077
KflI GGGWCCC 1 cut(s) 2572
Kpn2I TCCGGA 1 cut(s) 1111
KpnI GGTACC 1 cut(s) 1766
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
LguI GCTCTTC 1 cut(s) 981
Lsp1109I GCAGC 4 cut(s) 1028, 2248, 2626, 3109
LweI GCATC 5 cut(s) 282, 1697, 1804, 2221, 3109
MaeI CTAG 8 cut(s) 168, 186, 270, 378, 1134, 2267, 2325, 2939
MaeII ACGT 7 cut(s) 6, 320, 406, 541, 1519, 2043, 2361
MaeIII GTNAC 6 cut(s) 265, 402, 1292, 1645, 1923, 1996
MalI GATC 8 cut(s) 627, 1276, 1472, 2290, 2355, 2685, 2808, 2868
MbiI CCGCTC 2 cut(s) 1495, 1810
MboI GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
MfeI CAATTG 1 cut(s) 1254
MflI RGATCY 5 cut(s) 1274, 1470, 2288, 2683, 2806
MhlI GDGCHC 5 cut(s) 348, 1729, 2425, 2875, 3030
MlsI TGGCCA 1 cut(s) 2840
MluNI TGGCCA 1 cut(s) 2840
MlyI GAGTC 2 cut(s) 404, 1931
MmeI TCCRAC 3 cut(s) 501, 1217, 1957
Mox20I TGGCCA 1 cut(s) 2840
Mph1103I ATGCAT 1 cut(s) 1819
MroI TCCGGA 1 cut(s) 1111
MroXI GAANNNNTTC 3 cut(s) 642, 863, 2110
MscI TGGCCA 1 cut(s) 2840
MseI TTAA 8 cut(s) 648, 705, 1143, 1502, 1716, 2027, 2085, 2714
MslI CAYNNNNRTG 6 cut(s) 1401, 1734, 1799, 1898, 2313, 3274
Msp20I TGGCCA 1 cut(s) 2840
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 3 cut(s) 1112, 1635, 3021
MspR9I CCNGG 4 cut(s) 231, 1487, 2341, 3163
MunI CAATTG 1 cut(s) 1254
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 4 cut(s) 231, 1487, 2341, 3163
NcoI CCATGG 1 cut(s) 2536
NdeI CATATG 1 cut(s) 1819
NdeII GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
NmeAIII GCCGAG 1 cut(s) 1033
NmuCI GTSAC 3 cut(s) 402, 1292, 1923
NsiI ATGCAT 1 cut(s) 1819
NspI RCATGY 1 cut(s) 2750
NspV TTCGAA 1 cut(s) 3013
OliI CACNNNNGTG 2 cut(s) 1734, 3274
PaeI GCATGC 1 cut(s) 2750
PaeR7I CTCGAG 1 cut(s) 941
PagI TCATGA 1 cut(s) 1402
PceI AGGCCT 2 cut(s) 220, 1983
PciSI GCTCTTC 1 cut(s) 981
PctI GAATGC 1 cut(s) 1378
PdmI GAANNNNTTC 3 cut(s) 642, 863, 2110
PfeI GAWTC 2 cut(s) 2589, 2730
PflFI GACNNNGTC 1 cut(s) 1832
PflMI CCANNNNNTGG 3 cut(s) 89, 1333, 1904
PfoI TCCNGGA 1 cut(s) 1485
PkrI GCNGC 5 cut(s) 1018, 2263, 2641, 3124, 3268
PleI GAGTC 2 cut(s) 403, 1930
PpsI GAGTC 2 cut(s) 403, 1930
Ppu21I YACGTR 3 cut(s) 407, 1520, 2362
PpuMI RGGWCCY 2 cut(s) 1187, 2572
PsiI TTATAA 1 cut(s) 1173
Psp5II RGGWCCY 2 cut(s) 1187, 2572
Psp6I CCWGG 4 cut(s) 229, 1485, 2339, 3161
PspEI GGTNACC 1 cut(s) 1996
PspGI CCWGG 4 cut(s) 229, 1485, 2339, 3161
PspOMI GGGCCC 2 cut(s) 344, 1725
PspPPI RGGWCCY 2 cut(s) 1187, 2572
PsrI GAACNNNNNNTAC 2 cut(s) 1092, 1124
PstI CTGCAG 2 cut(s) 1771, 2512
PsuI RGATCY 5 cut(s) 1274, 1470, 2288, 2683, 2806
PsyI GACNNNGTC 1 cut(s) 1832
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 6 cut(s) 1124, 1320, 1764, 1780, 2125, 2761
RsaNI GTAC 6 cut(s) 1123, 1319, 1763, 1779, 2124, 2760
RseI CAYNNNNRTG 6 cut(s) 1401, 1734, 1799, 1898, 2313, 3274
SalI GTCGAC 1 cut(s) 2039
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 8 cut(s) 648, 705, 1143, 1502, 1716, 2027, 2085, 2714
SatI GCNGC 5 cut(s) 1017, 2262, 2640, 3123, 3267
Sau3AI GATC 8 cut(s) 625, 1274, 1470, 2288, 2353, 2683, 2806, 2866
SchI GAGTC 2 cut(s) 404, 1931
ScrFI CCNGG 4 cut(s) 231, 1487, 2341, 3163
SduI GDGCHC 5 cut(s) 348, 1729, 2425, 2875, 3030
SfaNI GCATC 5 cut(s) 282, 1697, 1804, 2221, 3109
SfcI CTRYAG 5 cut(s) 327, 1474, 1767, 2508, 2691
Sfr274I CTCGAG 1 cut(s) 941
SfuI TTCGAA 1 cut(s) 3013
SinI GGWCC 6 cut(s) 233, 1187, 1361, 1627, 1739, 2572
SlaI CTCGAG 1 cut(s) 941
SmiMI CAYNNNNRTG 6 cut(s) 1401, 1734, 1799, 1898, 2313, 3274
SmlI CTYRAG 5 cut(s) 580, 609, 920, 941, 1622
SmoI CTYRAG 5 cut(s) 580, 609, 920, 941, 1622
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 2750
SseBI AGGCCT 2 cut(s) 220, 1983
SsiI CCGC 7 cut(s) 39, 1364, 1495, 1732, 1808, 2204, 2234
SspI AATATT 2 cut(s) 1448, 2952
SspMI CTAG 8 cut(s) 168, 186, 270, 378, 1134, 2267, 2325, 2939
StuI AGGCCT 2 cut(s) 220, 1983
StyD4I CCNGG 4 cut(s) 229, 1485, 2339, 3161
StyI CCWWGG 5 cut(s) 1524, 1984, 2536, 2643, 2763
TaiI ACGT 7 cut(s) 9, 323, 409, 544, 1522, 2046, 2364
TaqI TCGA 6 cut(s) 942, 1812, 2040, 2710, 3013, 3243
TfiI GAWTC 2 cut(s) 2589, 2730
Tru1I TTAA 8 cut(s) 648, 705, 1143, 1502, 1716, 2027, 2085, 2714
Tru9I TTAA 8 cut(s) 648, 705, 1143, 1502, 1716, 2027, 2085, 2714
TseFI GTSAC 3 cut(s) 402, 1292, 1923
TseI GCWGC 5 cut(s) 1016, 2261, 2639, 3122, 3266
Tsp45I GTSAC 3 cut(s) 402, 1292, 1923
TspGWI ACGGA 2 cut(s) 1698, 3126
Tth111I GACNNNGTC 1 cut(s) 1832
Van91I CCANNNNNTGG 3 cut(s) 89, 1333, 1904
VpaK11BI GGWCC 6 cut(s) 233, 1187, 1361, 1627, 1739, 2572
XagI CCTNNNNNAGG 1 cut(s) 2694
XapI RAATTY 3 cut(s) 2090, 2414, 2833
XbaI TCTAGA 1 cut(s) 2266
XceI RCATGY 1 cut(s) 2750
XhoI CTCGAG 1 cut(s) 941
XmiI GTMKAC 3 cut(s) 1608, 2040, 2169
XmnI GAANNNNTTC 3 cut(s) 642, 863, 2110
XspI CTAG 8 cut(s) 168, 186, 270, 378, 1134, 2267, 2325, 2939
ZraI GACGTC 1 cut(s) 2044
Zsp2I ATGCAT 1 cut(s) 1819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.