Rroxscaffold_1G00072920

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003232
Physical Location & Seq
Forward (+)
93896547 .. 93904369
7823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072920.1

Sequence Viewer

Length: 2796 bp
ATGGAAGAAAAACAACGTATAGAGGAGATTGAGAGGTTAGCTGAGGAAAACTTCTCAAGAGGGAAGAAGCTGTTTGTCTATCCTCAAGTGGTGAAACCTGATCAACATATAGAAGTGTTTCTTAATAAAAGTCTTTCCACCCTAAACAGTGAAGATGATGTTTTCATCATGGGAGCCTTTAATGAATGGCGCTGGAAATCTTTTACCTTTCGGTTGAGCAGAACAAATCTCAAAGGGGATTGGTGGTCTTGTCAATTTCATGTTCCTGCCGAAGCATATAAGATAGACTTTGTGTTCTTCAATGGAAAGGATGTTTATGACAATAATGATAAGAAGGATTTCTGCATAGATGTAGAAAGTGGAATGGACGCCTTTGCATTTGAAGATTTCTTACTTGAGGAGAAGCGTAAAGAACTTGAGAAACTTGCAGAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATTGAAGCCGAGAAAGCAGCACAAGAAGCTGACAGAGCAGAGGCAAAGGCAGAGACTGAAAGGAGACGAAAATTGGTGCAGGAGTTGACCAAAAAGGCTGTAAGGTCTGTGCCGGATGTTTGGTACATAGAGCCTAGTGAGTTTAAAGGTAAGGACTTGGTGAGGTTATATTATAACCGAAGCTCAGGTCCTCTTGCTGGTGCTAATGAACTATGGGTTCATGGGGGACATAATGGTTGGAGTGCTGGCTTATCAATTGTTGAAAGTCTTGTTAGATCTGAGGAAAAGGATGTTGACTGGTGGTATGCCAAAGTTGTTGTACCTGACCAAGCTGTTGTATTGGATTGGGTTTTTGCTGATGGACCGCCTCAGAATGCCACTGTATATGATAACAATCATCGTCATGATTTCCATGCAGTAGTTCCAAAGTCCATTCCTCAGGAACAATATTGGGTTGAGGAAGAACACCAGATCTATAGGAAGCTCCAGGAGGAGCGGAGATTAAGGGAGGAGGCCATACATGCCAAGGCCAAAAAAACAGCACTTATGAAAGCAAAAATGAAGGAACGAACTCTGAGAAGGTATCTGCTGTCTCAGAAGCATATAGTCTACACTGAGCCTCTTGATGTCCAAGCCGGAAGGATGGTAATGATTTTCTACAATCCTGCCAATACTGCTTTGCACGGAAAGCCTGAGGTCTGGTTCAGATGCTCCTTTAATCATTGGGCCCACCGCAGTGGTCCCTTGCCACCACAGAAAATGGTACCTGCAGAGAATGGTACTCATGTCAAAACCACTGTCATGGTTCCGCTCGATGCATATGTGATGGACTTTGTCTTCTCTGAGAGTGAAGAAGGTGGAGTTTTTGACAACAAAAATGACATGGATTATCACATTCCAGTGTTTGGAGGAGTTTTGAAGGAGTCACCAATGCACATTGTGCATATTACGGTTGAAATGGCACCCATTGCAAAGGTTGGAGGCCTTGGTGATGTGGTTACCAGTCTAGCCCGAGCTGTGCAAGACTTAAATCACCATGTCGACGTCATTCTTCCCAAGTATGACTGTTTGAACCTTAGCAATGTTAAGGAATTTCAGTATAAAAGAAGCTATTCTTGGGGTGGTACTGAAATCAAAGTTTGGTTTGGGAAAGTGGAAGGAGTTTCAGTGTACTTTTTGGAACCTCAAAATGGACTGTTTTATGCGGGTTGTATATATGGTTGTAAGAATGATGCGCAGAGATTTGGCTTCTTTTGCCATGCTGCTCTAGAATATCTACTCCAAAGTGGATCCCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCGCCTGTTGCCTGGTTATATAAAGATCATTACGTGCATTATGGTCTTAGTAAAGCTCGCATTGTCTTCACAATTCATAATCTTGAATTTGGAGCACACTTTATTGGAAAAGCTGTGGCATACTCAGACAAGTCCACTACTGTATCCCACACTTACTCTAAGGAGATTGCTGGAAATCCTGCAGTTGCTCCACACCTCTACAAGTTCCATGGCATAATAAACGGAATTGACCAAGATTTATGGGATCCATACAATGATGGATTCCTACCTATATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGCAGATCTCCCTGTAGTAGGTATTATTACTCGATTAACTCAGCAGAAAGGAATCCATCTTATCAAGCATGCCATTTGGCGTACCTTGGAACGCAATGGACAGGTTGTATTGCTTGGTTCAGCTCCAGATCCTCGTATCCAAAATGAGTTTGTAAATTTGGCCAATCAATTACATTCTTCTCATGGTGATCGTGCTCGTCTTTGTTTGACTTATGATGAGCCACTCTCACATTTGATATACGCTGGTGCTGATTTCATCCTAGTTCCTTCAATATTTGAGCCATGCGGACTGACCCAACTTGTAGCAATGAGATACGGTTCCATACCTGTTGTTCGAAAAAACCGGAGTAAGCAAATTGACATGTGTATGGTCTCTGTTTCTTTGGTGGGGCTCTACGACACTGTATTTGATGTTGACCATGATAAAGAGAGAGCAGAGGCGCAGGGTCTTGAACCAAATGGGTTCAGTTTTGACGGAGCTGATGCTGCTGGTGTTGATTATGCTCTAAATAGAGCTATCTCTGCCTGGTATGATGGTCGGGATTGGTTCAACTCGTTGTGCAAAACAGTGATGGAGCAAGATTGGTCTTGGAACCGACCAGCTCTCGATTATATGGAGCTGTATCACGCAGCACGGAAGTGA

Protein Analysis

931

Amino Acids

106.66

Weight (kDa)

5.98

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 38 - 97 2.4e-11 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 212 - 274 7.6e-13 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 376 - 436 2.2e-09 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 470 - 658 7.6e-48 Starch synthase catalytic domain
Glyco_transf_4 PF13439 484 - 658 8.7e-09 Glycosyltransferase Family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AatII GACGTC 1 cut(s) 1521
Acc16I TGCGCA 1 cut(s) 1710
Acc36I ACCTGC 1 cut(s) 1249
Acc65I GGTACC 1 cut(s) 1237
AccB1I GGYRCC 2 cut(s) 1237, 1435
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccBSI CCGCTC 2 cut(s) 970, 1285
AccI GTMKAC 2 cut(s) 1083, 1515
AciI CCGC 6 cut(s) 839, 970, 1207, 1283, 1679, 2439
AclWI GGATC 5 cut(s) 1758, 1771, 2042, 2055, 2276
AcoI YGGCCR 1 cut(s) 2313
AcsI RAATTY 3 cut(s) 1565, 1889, 2308
AcyI GRCGYC 2 cut(s) 369, 1518
AdeI CACNNNGTG 1 cut(s) 1414
AfaI GTAC 7 cut(s) 599, 795, 1239, 1255, 1600, 1646, 2236
AfiI CCNNNNNNNGG 4 cut(s) 671, 1379, 1664, 2171
AflIII ACRYGT 1 cut(s) 2514
AjnI CCWGG 3 cut(s) 960, 1814, 2678
AjuI GAANNNNNNNTTGG 2 cut(s) 1573, 1605
AleI CACNNNNGTG 2 cut(s) 1209, 2791
Alw21I GWGCWC 2 cut(s) 1900, 2350
Alw26I GTCTC 4 cut(s) 521, 532, 1071, 2530
AlwI GGATC 5 cut(s) 1758, 1771, 2042, 2055, 2276
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 1485
AoxI GGCC 5 cut(s) 987, 1002, 1200, 1456, 2313
ApaI GGGCCC 1 cut(s) 1204
ApeKI GCWGC 5 cut(s) 491, 1736, 2114, 2639, 2783
ApoI RAATTY 3 cut(s) 1565, 1889, 2308
ArsI GACNNNNNNTTYG 8 cut(s) 646, 678, 1257, 1289, 2128, 2160, 2543, 2575
Asp700I GAANNNNTTC 3 cut(s) 117, 338, 1585
Asp718I GGTACC 1 cut(s) 1237
AspLEI GCGC 4 cut(s) 192, 1711, 1807, 2596
AspS9I GGNCC 5 cut(s) 662, 836, 1200, 1201, 1214
AsuHPI GGTGA 6 cut(s) 103, 646, 1392, 1475, 1499, 2351
AsuII TTCGAA 1 cut(s) 2488
AvaI CYCGRG 1 cut(s) 1485
AvaII GGWCC 3 cut(s) 662, 836, 1214
AxyI CCTNAGG 2 cut(s) 912, 1167
BaeGI GKGCMC 1 cut(s) 1204
BaeI ACNNNNGTAYC 4 cut(s) 1245, 1278, 1929, 1962
BalI TGGCCA 1 cut(s) 2315
BamHI GGATCC 2 cut(s) 1763, 2047
BanI GGYRCC 2 cut(s) 1237, 1435
BanII GRGCYC 2 cut(s) 1204, 2547
BarI GAAGNNNNNNTAC 2 cut(s) 375, 407
BbsI GAAGAC 2 cut(s) 1303, 1861
Bbv12I GWGCWC 2 cut(s) 1900, 2350
BbvCI CCTCAGC 1 cut(s) 42
BbvI GCAGC 4 cut(s) 503, 1723, 2101, 2626
BccI CCATC 8 cut(s) 827, 1111, 1294, 1776, 2054, 2217, 2681, 2719
BciT130I CCWGG 3 cut(s) 962, 1816, 2680
BciVI GTATCC 2 cut(s) 1957, 2300
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 4 cut(s) 521, 532, 1071, 2530
BfaI CTAG 5 cut(s) 609, 1481, 1742, 1800, 2414
BfmI CTRYAG 4 cut(s) 949, 1242, 1983, 2166
BfoI RGCGCY 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 1249
BfuI GTATCC 2 cut(s) 1957, 2300
BglII AGATCT 3 cut(s) 749, 945, 2158
BisI GCNGC 5 cut(s) 492, 1737, 2115, 2640, 2784
BlsI GCNGC 5 cut(s) 493, 1738, 2116, 2641, 2785
Bme1390I CCNGG 3 cut(s) 962, 1816, 2680
Bme18I GGWCC 3 cut(s) 662, 836, 1214
BmeT110I CYCGRG 1 cut(s) 1485
BmgT120I GGNCC 5 cut(s) 662, 836, 1200, 1201, 1214
BmrFI CCNGG 3 cut(s) 962, 1816, 2680
BmsI GCATC 4 cut(s) 1172, 1279, 1696, 2626
BpiI GAAGAC 2 cut(s) 1303, 1861
BplI GAGNNNNNCTC 2 cut(s) 2363, 2395
BpmI CTGGAG 2 cut(s) 944, 2262
Bpu10I CCTNAGC 3 cut(s) 42, 658, 1550
Bpu14I TTCGAA 1 cut(s) 2488
BpuEI CTTGAG 4 cut(s) 40, 69, 416, 437
BsaAI YACGTR 1 cut(s) 1837
BsaBI GATNNNNATC 1 cut(s) 867
BsaHI GRCGYC 2 cut(s) 369, 1518
BsaI GGTCTC 1 cut(s) 2530
BsaJI CCNNGG 5 cut(s) 999, 1459, 2011, 2118, 2238
BsaWI WCCGGW 1 cut(s) 2496
BsaXI ACNNNNNCTCC 2 cut(s) 1626, 1656
Bsc4I CCNNNNNNNGG 4 cut(s) 671, 1379, 1664, 2171
Bse1I ACTGG 3 cut(s) 776, 1373, 1476
Bse21I CCTNAGG 2 cut(s) 912, 1167
Bse3DI GCAATG 5 cut(s) 1440, 1561, 1783, 2254, 2466
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 3 cut(s) 962, 1816, 2680
BseDI CCNNGG 5 cut(s) 999, 1459, 2011, 2118, 2238
BseGI GGATG 6 cut(s) 316, 595, 769, 1122, 1768, 2409
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 4 cut(s) 671, 1379, 1664, 2171
BseMI GCAATG 5 cut(s) 1440, 1561, 1783, 2254, 2466
BseNI ACTGG 3 cut(s) 776, 1373, 1476
BseRI GAGGAG 6 cut(s) 38, 413, 446, 980, 998, 1398
BseSI GKGCMC 1 cut(s) 1204
BseXI GCAGC 4 cut(s) 503, 1723, 2101, 2626
BsgI GTGCAG 1 cut(s) 572
BshFI GGCC 5 cut(s) 989, 1004, 1202, 1458, 2315
BshNI GGYRCC 2 cut(s) 1237, 1435
BsiHKAI GWGCWC 2 cut(s) 1900, 2350
BsiHKCI CYCGRG 1 cut(s) 1485
BsiSI CCGG 3 cut(s) 587, 1110, 2497
BslFI GGGAC 2 cut(s) 714, 1200
BslI CCNNNNNNNGG 4 cut(s) 671, 1379, 1664, 2171
BsmAI GTCTC 4 cut(s) 521, 532, 1071, 2530
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 714, 1200
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 5 cut(s) 989, 1004, 1202, 1458, 2315
Bso31I GGTCTC 1 cut(s) 2530
BsoBI CYCGRG 1 cut(s) 1485
Bsp119I TTCGAA 1 cut(s) 2488
Bsp120I GGGCCC 1 cut(s) 1200
Bsp1286I GDGCHC 4 cut(s) 1204, 1900, 2350, 2547
Bsp143I GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
Bsp19I CCATGG 1 cut(s) 2011
BspACI CCGC 6 cut(s) 839, 970, 1207, 1283, 1679, 2439
BspANI GGCC 5 cut(s) 989, 1004, 1202, 1458, 2315
BspHI TCATGA 1 cut(s) 877
BspMAI CTGCAG 2 cut(s) 1246, 1987
BspMI ACCTGC 1 cut(s) 1249
BspPI GGATC 5 cut(s) 1758, 1771, 2042, 2055, 2276
BspQI GCTCTTC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 2488
BspT107I GGYRCC 2 cut(s) 1237, 1435
BspTNI GGTCTC 1 cut(s) 2530
BsrBI CCGCTC 2 cut(s) 970, 1285
BsrDI GCAATG 5 cut(s) 1440, 1561, 1783, 2254, 2466
BsrI ACTGG 3 cut(s) 776, 1373, 1476
BssECI CCNNGG 5 cut(s) 999, 1459, 2011, 2118, 2238
BssMI GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
BssNI GRCGYC 2 cut(s) 369, 1518
BssT1I CCWWGG 5 cut(s) 999, 1459, 2011, 2118, 2238
Bst2UI CCWGG 3 cut(s) 962, 1816, 2680
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 2 cut(s) 369, 1518
BstAPI GCANNNNNTGC 2 cut(s) 1414, 1442
BstBAI YACGTR 1 cut(s) 1837
BstBI TTCGAA 1 cut(s) 2488
BstC8I GCNNGC 5 cut(s) 438, 456, 721, 1861, 2223
BstDSI CCRYGG 1 cut(s) 2011
BstEII GGTNACC 1 cut(s) 1471
BstENI CCTNNNNNAGG 1 cut(s) 2169
BstF5I GGATG 6 cut(s) 316, 595, 769, 1122, 1768, 2409
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 4 cut(s) 192, 1711, 1807, 2596
BstKTI GATC 9 cut(s) 103, 752, 948, 1766, 1831, 2050, 2161, 2284, 2344
BstMAI GTCTC 4 cut(s) 521, 532, 1071, 2530
BstMBI GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
BstNI CCWGG 3 cut(s) 962, 1816, 2680
BstNSI RCATGY 3 cut(s) 998, 2225, 2518
BstPI GGTNACC 1 cut(s) 1471
BstSCI CCNGG 3 cut(s) 960, 1814, 2678
BstSFI CTRYAG 4 cut(s) 949, 1242, 1983, 2166
BstSLI GKGCMC 1 cut(s) 1204
BstV1I GCAGC 4 cut(s) 503, 1723, 2101, 2626
BstV2I GAAGAC 2 cut(s) 1303, 1861
BstX2I RGATCY 6 cut(s) 749, 945, 1763, 2047, 2158, 2281
BstXI CCANNNNNNTGG 2 cut(s) 1211, 1276
BstYI RGATCY 6 cut(s) 749, 945, 1763, 2047, 2158, 2281
Bsu36I CCTNAGG 2 cut(s) 912, 1167
BsuI GTATCC 2 cut(s) 1957, 2300
BsuRI GGCC 5 cut(s) 989, 1004, 1202, 1458, 2315
BtgI CCRYGG 1 cut(s) 2011
BtsCI GGATG 6 cut(s) 316, 595, 769, 1122, 1768, 2409
BtsI GCAGTG 1 cut(s) 1216
BtsIMutI CAGTG 9 cut(s) 154, 852, 1086, 1216, 1269, 1380, 1647, 2553, 2727
BveI ACCTGC 1 cut(s) 1249
Cac8I GCNNGC 5 cut(s) 438, 456, 721, 1861, 2223
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 877
CfoI GCGC 4 cut(s) 192, 1711, 1807, 2596
Cfr13I GGNCC 5 cut(s) 662, 836, 1200, 1201, 1214
CseI GACGC 1 cut(s) 377
Csp6I GTAC 7 cut(s) 598, 794, 1238, 1254, 1599, 1645, 2235
CviQI GTAC 7 cut(s) 598, 794, 1238, 1254, 1599, 1645, 2235
DpnI GATC 9 cut(s) 102, 751, 947, 1765, 1830, 2049, 2160, 2283, 2343
DpnII GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
DraI TTTAAA 1 cut(s) 619
DraIII CACNNNGTG 1 cut(s) 1414
EaeI YGGCCR 1 cut(s) 2313
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 5 cut(s) 999, 1459, 2011, 2118, 2238
Eco147I AGGCCT 1 cut(s) 1458
Eco24I GRGCYC 2 cut(s) 1204, 2547
Eco31I GGTCTC 1 cut(s) 2530
Eco32I GATATC 1 cut(s) 1777
Eco47I GGWCC 3 cut(s) 662, 836, 1214
Eco81I CCTNAGG 2 cut(s) 912, 1167
Eco88I CYCGRG 1 cut(s) 1485
Eco91I GGTNACC 1 cut(s) 1471
EcoNI CCTNNNNNAGG 1 cut(s) 2169
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 1471
EcoRII CCWGG 3 cut(s) 960, 1814, 2678
EcoRV GATATC 1 cut(s) 1777
EcoT14I CCWWGG 5 cut(s) 999, 1459, 2011, 2118, 2238
EcoT22I ATGCAT 1 cut(s) 1294
EcoT38I GRGCYC 2 cut(s) 1204, 2547
ErhI CCWWGG 5 cut(s) 999, 1459, 2011, 2118, 2238
Esp3I CGTCTC 1 cut(s) 532
FalI AAGNNNNNCTT 8 cut(s) 105, 137, 272, 304, 1573, 1605, 2111, 2143
FaqI GGGAC 2 cut(s) 714, 1200
FauI CCCGC 1 cut(s) 1672
FauNDI CATATG 1 cut(s) 1294
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 2 cut(s) 1083, 1515
Fnu4HI GCNGC 5 cut(s) 492, 1737, 2115, 2640, 2784
FokI GGATG 6 cut(s) 323, 602, 776, 1129, 1755, 2396
FriOI GRGCYC 2 cut(s) 1204, 2547
Fsp4HI GCNGC 5 cut(s) 492, 1737, 2115, 2640, 2784
FspBI CTAG 5 cut(s) 609, 1481, 1742, 1800, 2414
FspI TGCGCA 1 cut(s) 1710
GlaI GCGC 4 cut(s) 191, 1710, 1806, 2595
GluI GCNGC 5 cut(s) 492, 1737, 2115, 2640, 2784
GsuI CTGGAG 2 cut(s) 944, 2262
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 5 cut(s) 989, 1004, 1202, 1458, 2315
HapII CCGG 3 cut(s) 587, 1110, 2497
HgaI GACGC 1 cut(s) 377
HhaI GCGC 4 cut(s) 192, 1711, 1807, 2596
Hin1I GRCGYC 2 cut(s) 369, 1518
Hin6I GCGC 4 cut(s) 190, 1709, 1805, 2594
HinP1I GCGC 4 cut(s) 190, 1709, 1805, 2594
HincII GTYRAC 4 cut(s) 561, 769, 1516, 2569
HindII GTYRAC 4 cut(s) 561, 769, 1516, 2569
HinfI GANTC 3 cut(s) 1397, 2064, 2205
HpaII CCGG 3 cut(s) 587, 1110, 2497
HphI GGTGA 6 cut(s) 103, 646, 1392, 1475, 1499, 2351
Hpy166II GTNNAC 7 cut(s) 561, 769, 1084, 1516, 1645, 1938, 2569
Hpy188I TCNGA 9 cut(s) 754, 846, 1050, 1071, 1181, 1318, 1930, 2089, 2148
Hpy8I GTNNAC 7 cut(s) 561, 769, 1084, 1516, 1645, 1938, 2569
Hpy99I CGWCG 1 cut(s) 1520
HpyAV CCTTC 9 cut(s) 328, 1030, 1047, 1107, 1322, 1387, 1625, 2096, 2430
HpyCH4IV ACGT 3 cut(s) 16, 1518, 1836
HpySE526I ACGT 3 cut(s) 16, 1518, 1836
Hsp92I GRCGYC 2 cut(s) 369, 1518
HspAI GCGC 4 cut(s) 190, 1709, 1805, 2594
KpnI GGTACC 1 cut(s) 1241
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
LguI GCTCTTC 1 cut(s) 456
Lsp1109I GCAGC 4 cut(s) 503, 1723, 2101, 2626
LweI GCATC 4 cut(s) 1172, 1279, 1696, 2626
MaeI CTAG 5 cut(s) 609, 1481, 1742, 1800, 2414
MaeII ACGT 3 cut(s) 16, 1518, 1836
MaeIII GTNAC 2 cut(s) 1398, 1471
MalI GATC 9 cut(s) 102, 751, 947, 1765, 1830, 2049, 2160, 2283, 2343
MbiI CCGCTC 2 cut(s) 970, 1285
MboI GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 6 cut(s) 749, 945, 1763, 2047, 2158, 2281
MhlI GDGCHC 4 cut(s) 1204, 1900, 2350, 2547
MlsI TGGCCA 1 cut(s) 2315
MluNI TGGCCA 1 cut(s) 2315
MlyI GAGTC 1 cut(s) 1406
MmeI TCCRAC 2 cut(s) 692, 1432
Mox20I TGGCCA 1 cut(s) 2315
Mph1103I ATGCAT 1 cut(s) 1294
MroXI GAANNNNTTC 3 cut(s) 117, 338, 1585
MscI TGGCCA 1 cut(s) 2315
MseI TTAA 8 cut(s) 123, 180, 618, 977, 1191, 1502, 1560, 2189
MslI CAYNNNNRTG 7 cut(s) 876, 1209, 1274, 1373, 1788, 2519, 2791
Msp20I TGGCCA 1 cut(s) 2315
MspI CCGG 3 cut(s) 587, 1110, 2497
MspR9I CCNGG 3 cut(s) 962, 1816, 2680
MunI CAATTG 1 cut(s) 729
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 3 cut(s) 962, 1816, 2680
NcoI CCATGG 1 cut(s) 2011
NdeI CATATG 1 cut(s) 1294
NdeII GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
NmeAIII GCCGAG 1 cut(s) 508
NmuCI GTSAC 1 cut(s) 1398
NsbI TGCGCA 1 cut(s) 1710
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 3 cut(s) 998, 2225, 2518
NspV TTCGAA 1 cut(s) 2488
OliI CACNNNNGTG 2 cut(s) 1209, 2791
PaeI GCATGC 1 cut(s) 2225
PagI TCATGA 1 cut(s) 877
PceI AGGCCT 1 cut(s) 1458
PciI ACATGT 1 cut(s) 2514
PciSI GCTCTTC 1 cut(s) 456
PctI GAATGC 1 cut(s) 853
PdmI GAANNNNTTC 3 cut(s) 117, 338, 1585
PfeI GAWTC 2 cut(s) 2064, 2205
PflFI GACNNNGTC 1 cut(s) 1307
PflMI CCANNNNNTGG 1 cut(s) 1379
PfoI TCCNGGA 1 cut(s) 960
PkrI GCNGC 5 cut(s) 493, 1738, 2116, 2641, 2785
PleI GAGTC 1 cut(s) 1405
PpsI GAGTC 1 cut(s) 1405
Ppu21I YACGTR 1 cut(s) 1837
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 1 cut(s) 2514
PsiI TTATAA 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 960, 1814, 2678
PspEI GGTNACC 1 cut(s) 1471
PspGI CCWGG 3 cut(s) 960, 1814, 2678
PspOMI GGGCCC 1 cut(s) 1200
PspPI GGNCC 5 cut(s) 662, 836, 1200, 1201, 1214
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 2 cut(s) 1246, 1987
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 6 cut(s) 749, 945, 1763, 2047, 2158, 2281
PsyI GACNNNGTC 1 cut(s) 1307
RsaI GTAC 7 cut(s) 599, 795, 1239, 1255, 1600, 1646, 2236
RsaNI GTAC 7 cut(s) 598, 794, 1238, 1254, 1599, 1645, 2235
RseI CAYNNNNRTG 7 cut(s) 876, 1209, 1274, 1373, 1788, 2519, 2791
SalI GTCGAC 1 cut(s) 1514
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 8 cut(s) 123, 180, 618, 977, 1191, 1502, 1560, 2189
SatI GCNGC 5 cut(s) 492, 1737, 2115, 2640, 2784
Sau3AI GATC 9 cut(s) 100, 749, 945, 1763, 1828, 2047, 2158, 2281, 2341
Sau96I GGNCC 5 cut(s) 662, 836, 1200, 1201, 1214
SchI GAGTC 1 cut(s) 1406
ScrFI CCNGG 3 cut(s) 962, 1816, 2680
SduI GDGCHC 4 cut(s) 1204, 1900, 2350, 2547
SfaNI GCATC 4 cut(s) 1172, 1279, 1696, 2626
SfcI CTRYAG 4 cut(s) 949, 1242, 1983, 2166
SfuI TTCGAA 1 cut(s) 2488
SinI GGWCC 3 cut(s) 662, 836, 1214
SmiMI CAYNNNNRTG 7 cut(s) 876, 1209, 1274, 1373, 1788, 2519, 2791
SmlI CTYRAG 4 cut(s) 55, 84, 395, 416
SmoI CTYRAG 4 cut(s) 55, 84, 395, 416
SphI GCATGC 1 cut(s) 2225
SseBI AGGCCT 1 cut(s) 1458
SsiI CCGC 6 cut(s) 839, 970, 1207, 1283, 1679, 2439
SspI AATATT 2 cut(s) 923, 2427
SspMI CTAG 5 cut(s) 609, 1481, 1742, 1800, 2414
StuI AGGCCT 1 cut(s) 1458
StyD4I CCNGG 3 cut(s) 960, 1814, 2678
StyI CCWWGG 5 cut(s) 999, 1459, 2011, 2118, 2238
TaiI ACGT 3 cut(s) 19, 1521, 1839
TaqI TCGA 5 cut(s) 1287, 1515, 2185, 2488, 2760
TatI WGTACW 1 cut(s) 1644
TfiI GAWTC 2 cut(s) 2064, 2205
Tru1I TTAA 8 cut(s) 123, 180, 618, 977, 1191, 1502, 1560, 2189
Tru9I TTAA 8 cut(s) 123, 180, 618, 977, 1191, 1502, 1560, 2189
TscAI CASTG 9 cut(s) 154, 859, 1093, 1216, 1276, 1380, 1647, 2560, 2727
TseFI GTSAC 1 cut(s) 1398
TseI GCWGC 5 cut(s) 491, 1736, 2114, 2639, 2783
Tsp45I GTSAC 1 cut(s) 1398
TspGWI ACGGA 3 cut(s) 1173, 2040, 2643
TspRI CASTG 9 cut(s) 154, 859, 1093, 1216, 1276, 1380, 1647, 2560, 2727
Tth111I GACNNNGTC 1 cut(s) 1307
Van91I CCANNNNNTGG 1 cut(s) 1379
VpaK11BI GGWCC 3 cut(s) 662, 836, 1214
XagI CCTNNNNNAGG 1 cut(s) 2169
XapI RAATTY 3 cut(s) 1565, 1889, 2308
XbaI TCTAGA 1 cut(s) 1741
XceI RCATGY 3 cut(s) 998, 2225, 2518
XmiI GTMKAC 2 cut(s) 1083, 1515
XmnI GAANNNNTTC 3 cut(s) 117, 338, 1585
XspI CTAG 5 cut(s) 609, 1481, 1742, 1800, 2414
ZraI GACGTC 1 cut(s) 1519
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.