Rh5DG025000

Starch synthase 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003232
Physical Location & Seq
Reverse (-)
1742473 .. 1751965
9493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG025000.1

Sequence Viewer

Length: 3036 bp
ATGGACGTGTCTTTGCAAGCTCATAGGCCACTCAGTTGCAGAACAGTGTTTCAAGACAGCAGAAGCATTTTGAAGCTCAAACCGGTTACTGGGTTTTGTCCTAATGGAAGATTATCTTCATGGTTCAAAGGAGACCTGACAAGTGGGGCTTCATGTAAAATTACTGCTAGTTCAGCTGATTTCTCTAGGAGGAGACAGAAAAAAGTGTCAGGTTCAAGGCCTAATGGCCCTGGTCCAAAGGCAATAGTTCCCAAAACCCCAGTAGAAACTAGCATCCCAAAGACAAATCAAAGGAATACTGGGGATAAAAAGGGTTTTGCGTCTTCTACAGCCAGTGTTGTTGGGCCAAAAGTTGATGAAAAAAGGATTGATGAAACTAGTCGTAAAGTTGAGGAGTCTTCGTCACCAGCTACTGGTAGAAGCTTTCAAGAGGTAGAAAATGGGAGTATAGATAAAGGCATTGATATTGATAAGAAGTTATCTGATGAAGCTTCTTTGAAACTAAAGTTGGAGATGGAAGAAAAACAACGTATAGAGGAGATTGAGAGGTTAGCTGAGGAAAACTTCTCAAGAGGGAAGAAGCTGTTTGTCTATCCTCAAGTGGTGAAACCTGATCAACATATAGAAGTGTTTCTTAATAAAAGTCTTTCCACCCTAAACAGTGAAGATGATGTTTTCATCATGGGAGCCTTTAATGAATGGCGCTGGAAATCTTTTACCTTTCGGTTGAGCAGAACAAATCTCAAAGGGGATTGGTGGTCTTGTCAATTTCATGTTCCTGCCGAAGCATATAAGATAGACTTTGTGTTCTTCAATGGAAAAGATGTTTATGACAATAATGATAAGAAGGATTTCTGCATAGATGTAGAAAGTGGATTGGATGCCTTTGCATTTGAAGATTTCTTACTTGAGGAGAAGCGTAAAGAACTTGAGAAACTTGCAGAGGAGCAAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATTGAAGCCGAGAAAGCAGCACAAGAAGCTGACAGAGCAGAGGCAAAGGCAGAGACTGAAAGGAGACGAAAATTGGTGCAGGAGTTGACCAAAAAGGCTGTAAGGTCTGTTCCGGATGTTTGGTACATAGAGCCTAGTGAGTTTAAAGGTAAGGACTTGGTGAGGTTATATTATAACCGAAGCTCAGGTCCTCTTGCTGGTGCTAATGAACTATGGGTTCATGGGGGACATAATGGTTGGAGTGCTGGCTTATCAATTGTTGAAAGTCTTGTTAGATCTGAGGAAAAGGATGGTGACTGGTGGTATGCCAAAGTTGTTGTACCTGACCAAGCTGTTGTATTGGATTGGGTTTTTGCTGATGGACCGCCTCAGAATGCCACTGTATATGATAACAATCATCGTCATGATTTCCATGCAGTAGTTCCAAAGTCCATTCCTCAGGAACAATATTGGGTTGAGGAAGAACACCAGATCTATAGGAAGCTCCAGGAGGAGCGGAGATTAAGGGAGGAGGCCATACGTGCCAAGGCCAAAAAAACAGCACTTATGAAAGCAAAAATGAAGGAACGAACTCTGAGAAGGTATCTGCTGTCTCAGAAGCATATAGTCTACACTGAGCCTCTTGATGTCCAAGCCGGAAGCATGGTAACGATTTTCTACAATCCTGCCAATACTGCTTTGCACGGAAAGCCTGAGGTCTGGTTCAGATGCTCCTTTAACCATTGGGCCCACCGCAGTGGTCCCTTGCCACCACAGAAAATGGTACCTGCAGAGAATGGTACTCATGTCAAAACCACTGTCATGGTTCCGCTCGATGCATATGTGATGGACTTTGTCTTCTCTGAGAGTGAAGAAGGTGGAGTTTTTGACAACAAAAATGACATGGATTATCACATTCCAGTGTTTGGAGGAGTTTTGAAGGAGTCACCAATGCACATTGTGCATATTACGGTTGAAATGGCACCCATTGCAAAGGTTGGAGGCCTTGGTGATGTGGTTACCAGTCTATCCCGAGCTGTGCAAGACTTAAATCACCATGTCGACGTCATTCTTCCCAAGTATGACTGTTTGAACCTTAGCAATGTTAAGGAATTTCAGTATAAAAGAAGCTATTCTTGGGGTGGTACTGAAATCAAAGTTTGGTTTGGGAAAGTGGAAGGAGTTTCAGTCTACTTTTTGGAACCTCAAAATGGACTGTTTTATGCGGGTTGTATATATGGTTGTAAGAATGATGCGGAGAGATTTGGCTTCTTTTGCCATGCTGCTCTAGAATATCTACTCCAAAGTGGATCCCATCCTGATATCATTCATTGCCATGATTGGTCTAGTGCGCCTGTTACCTGGTTATATAAAGATCATTACATGCATTATGGTCTTAGTAAAGCTCGCATTTTCTTCACAATTCATAATCTTGAATTTGGAGCACGCTTTATTGGAAAAGCTGTGGCATACTCAGACAAGTCCACTACTGTATCCCACACTTACTCTAAGGAGATTGCTGGAAATCCTGCAGTAGCTCCACACCTCTACAAGTTCCATGGCATAATAAACGGAATTGACCAAGATATATGGGACCCATACAATGATGGATTCCTACCTATATCATATACTTCTGAAAATGTTGTTGAAGGCAAACAAGCTGCCAAGGAAGCCTTGCAACAAAGGCTTGGTCTGAAAACAGTAGATCTCCCTGTAGTAGGTATTATTTCTCGATTAACTCAGCAGAAAGGAATCCATCTTATCAAGCATGCCATTAGGCGTACCTTGGAACGCAGTGGACAGGTTGTATTGCTTGGTTCAGCTCCAGATCCTCGTATCCAAAATGAGTTTGTAAATTTGGCCAATCAATTACATTCTTCTCATGGTGATCGTGCTCGTCTTTGTTTGACTTATGATGAGCCACTCTCACATTTGATATACGCTGGTGCTGATTTCATGCTAGTTCCTTCAATATTTGAGCCATGTGGACTGACCCAACTTGTAGCAATGAGATACGGTTCCATACCTGTTGTTCGAAAAACCGGAGGTATGCACATACCTTATCAGTAA

Protein Analysis

1011

Amino Acids

114.66

Weight (kDa)

7.08

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM53 PF16760 209 - 268 2.6e-11 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 383 - 445 1.8e-13 Starch/carbohydrate-binding module (family 53)
CBM53 PF16760 545 - 607 2.2e-10 Starch/carbohydrate-binding module (family 53)
Glyco_transf_5 PF08323 641 - 829 2.4e-47 Starch synthase catalytic domain
Glyco_transf_4 PF13439 655 - 830 4.6e-07 Glycosyltransferase Family 4
Glyco_trans_1_4 PF13692 893 - 1005 3.2e-06 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AatII GACGTC 1 cut(s) 2034
Acc36I ACCTGC 1 cut(s) 1762
Acc65I GGTACC 1 cut(s) 1750
AccB1I GGYRCC 2 cut(s) 1750, 1948
AccB7I CCANNNNNTGG 2 cut(s) 413, 1892
AccBSI CCGCTC 2 cut(s) 1483, 1798
AccI GTMKAC 3 cut(s) 1596, 2028, 2157
AccIII TCCGGA 1 cut(s) 1099
AciI CCGC 6 cut(s) 1352, 1483, 1720, 1796, 2192, 2222
AclWI GGATC 3 cut(s) 2271, 2284, 2789
AcoI YGGCCR 1 cut(s) 2826
AcsI RAATTY 3 cut(s) 2078, 2402, 2821
AcyI GRCGYC 1 cut(s) 2031
AdeI CACNNNGTG 1 cut(s) 1927
AfaI GTAC 6 cut(s) 1112, 1308, 1752, 1768, 2113, 2749
AfiI CCNNNNNNNGG 6 cut(s) 89, 413, 1184, 1892, 2177, 2684
AflIII ACRYGT 1 cut(s) 6
AgeI ACCGGT 1 cut(s) 82
AhlI ACTAGT 1 cut(s) 377
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 3 cut(s) 229, 1473, 2327
AjuI GAANNNNNNNTTGG 4 cut(s) 491, 523, 2086, 2118
AleI CACNNNNGTG 1 cut(s) 1722
Alw21I GWGCWC 2 cut(s) 2413, 2863
Alw26I GTCTC 5 cut(s) 126, 187, 1034, 1045, 1584
AlwI GGATC 3 cut(s) 2271, 2284, 2789
AlwNI CAGNNNCTG 2 cut(s) 413, 1043
Ama87I CYCGRG 1 cut(s) 1998
Aor13HI TCCGGA 1 cut(s) 1099
AoxI GGCC 9 cut(s) 26, 218, 226, 344, 1500, 1515, 1713, 1969, 2826
ApaI GGGCCC 1 cut(s) 1717
ApeKI GCWGC 3 cut(s) 1004, 2249, 2627
ApoI RAATTY 3 cut(s) 2078, 2402, 2821
ArsI GACNNNNNNTTYG 6 cut(s) 1159, 1191, 1770, 1802, 2641, 2673
AsiGI ACCGGT 1 cut(s) 82
Asp700I GAANNNNTTC 3 cut(s) 630, 851, 2098
Asp718I GGTACC 1 cut(s) 1750
AspLEI GCGC 2 cut(s) 705, 2320
AspS9I GGNCC 9 cut(s) 227, 233, 344, 1175, 1349, 1713, 1714, 1727, 2560
AsuHPI GGTGA 8 cut(s) 396, 616, 1159, 1292, 1905, 1988, 2012, 2864
AsuII TTCGAA 1 cut(s) 3001
AvaI CYCGRG 1 cut(s) 1998
AvaII GGWCC 5 cut(s) 233, 1175, 1349, 1727, 2560
AxyI CCTNAGG 2 cut(s) 1425, 1680
BaeGI GKGCMC 1 cut(s) 1717
BaeI ACNNNNGTAYC 4 cut(s) 1758, 1791, 2442, 2475
BalI TGGCCA 1 cut(s) 2828
BamHI GGATCC 1 cut(s) 2276
BanI GGYRCC 2 cut(s) 1750, 1948
BanII GRGCYC 1 cut(s) 1717
BarI GAAGNNNNNNTAC 2 cut(s) 888, 920
BbsI GAAGAC 3 cut(s) 315, 390, 1816
Bbv12I GWGCWC 2 cut(s) 2413, 2863
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 3 cut(s) 1016, 2236, 2614
BccI CCATC 7 cut(s) 508, 1271, 1340, 1807, 2289, 2567, 2730
BciT130I CCWGG 3 cut(s) 231, 1475, 2329
BciVI GTATCC 2 cut(s) 2470, 2813
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 5 cut(s) 126, 187, 1034, 1045, 1584
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 8 cut(s) 168, 186, 270, 378, 1122, 2255, 2313, 2927
BfmI CTRYAG 5 cut(s) 327, 1462, 1755, 2496, 2679
BfoI RGCGCY 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 1762
BfuI GTATCC 2 cut(s) 2470, 2813
BglII AGATCT 3 cut(s) 1262, 1458, 2671
BisI GCNGC 3 cut(s) 1005, 2250, 2628
BlsI GCNGC 3 cut(s) 1006, 2251, 2629
Bme1390I CCNGG 3 cut(s) 231, 1475, 2329
Bme18I GGWCC 5 cut(s) 233, 1175, 1349, 1727, 2560
BmeT110I CYCGRG 1 cut(s) 1998
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 9 cut(s) 227, 233, 344, 1175, 1349, 1713, 1714, 1727, 2560
BmrFI CCNGG 3 cut(s) 231, 1475, 2329
BmrI ACTGGG 3 cut(s) 99, 254, 309
BmsI GCATC 5 cut(s) 282, 871, 1685, 1792, 2209
BmuI ACTGGG 3 cut(s) 99, 254, 309
BpiI GAAGAC 3 cut(s) 315, 390, 1816
BplI GAGNNNNNCTC 2 cut(s) 2876, 2908
BpmI CTGGAG 2 cut(s) 1457, 2775
Bpu10I CCTNAGC 3 cut(s) 555, 1171, 2063
Bpu14I TTCGAA 1 cut(s) 3001
BpuEI CTTGAG 4 cut(s) 553, 582, 929, 950
BsaAI YACGTR 1 cut(s) 1508
BsaBI GATNNNNATC 1 cut(s) 1380
BsaHI GRCGYC 1 cut(s) 2031
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 6 cut(s) 229, 1512, 1972, 2524, 2631, 2751
BsaWI WCCGGW 3 cut(s) 82, 1099, 3008
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 2139, 2169
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 413, 1184, 1892, 2177, 2684
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 8 cut(s) 94, 260, 304, 333, 418, 1289, 1886, 1989
Bse21I CCTNAGG 2 cut(s) 1425, 1680
Bse3DI GCAATG 4 cut(s) 1953, 2074, 2296, 2979
Bse8I GATNNNNATC 1 cut(s) 1380
BseAI TCCGGA 1 cut(s) 1099
BseBI CCWGG 3 cut(s) 231, 1475, 2329
BseDI CCNNGG 6 cut(s) 229, 1512, 1972, 2524, 2631, 2751
BseGI GGATG 5 cut(s) 273, 886, 1108, 1282, 2281
BseJI GATNNNNATC 1 cut(s) 1380
BseLI CCNNNNNNNGG 6 cut(s) 89, 413, 1184, 1892, 2177, 2684
BseMI GCAATG 4 cut(s) 1953, 2074, 2296, 2979
BseNI ACTGG 8 cut(s) 94, 260, 304, 333, 418, 1289, 1886, 1989
BseRI GAGGAG 8 cut(s) 205, 407, 551, 926, 959, 1493, 1511, 1911
BseSI GKGCMC 1 cut(s) 1717
BseXI GCAGC 3 cut(s) 1016, 2236, 2614
BsgI GTGCAG 1 cut(s) 1085
BshFI GGCC 9 cut(s) 28, 220, 228, 346, 1502, 1517, 1715, 1971, 2828
BshNI GGYRCC 2 cut(s) 1750, 1948
BshTI ACCGGT 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 2413, 2863
BsiHKCI CYCGRG 1 cut(s) 1998
BsiSI CCGG 4 cut(s) 83, 1100, 1623, 3009
BslFI GGGAC 3 cut(s) 1227, 1713, 2573
BslI CCNNNNNNNGG 6 cut(s) 89, 413, 1184, 1892, 2177, 2684
BsmAI GTCTC 5 cut(s) 126, 187, 1034, 1045, 1584
BsmBI CGTCTC 1 cut(s) 1045
BsmFI GGGAC 3 cut(s) 1227, 1713, 2573
BsmI GAATGC 1 cut(s) 1366
BsnI GGCC 9 cut(s) 28, 220, 228, 346, 1502, 1517, 1715, 1971, 2828
Bso31I GGTCTC 1 cut(s) 126
BsoBI CYCGRG 1 cut(s) 1998
Bsp119I TTCGAA 1 cut(s) 3001
Bsp120I GGGCCC 1 cut(s) 1713
Bsp1286I GDGCHC 3 cut(s) 1717, 2413, 2863
Bsp13I TCCGGA 1 cut(s) 1099
Bsp143I GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
Bsp19I CCATGG 1 cut(s) 2524
BspACI CCGC 6 cut(s) 1352, 1483, 1720, 1796, 2192, 2222
BspANI GGCC 9 cut(s) 28, 220, 228, 346, 1502, 1517, 1715, 1971, 2828
BspEI TCCGGA 1 cut(s) 1099
BspHI TCATGA 1 cut(s) 1390
BspMAI CTGCAG 2 cut(s) 1759, 2500
BspMI ACCTGC 1 cut(s) 1762
BspPI GGATC 3 cut(s) 2271, 2284, 2789
BspQI GCTCTTC 1 cut(s) 969
BspT104I TTCGAA 1 cut(s) 3001
BspT107I GGYRCC 2 cut(s) 1750, 1948
BspTNI GGTCTC 1 cut(s) 126
BsrBI CCGCTC 2 cut(s) 1483, 1798
BsrDI GCAATG 4 cut(s) 1953, 2074, 2296, 2979
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 8 cut(s) 94, 260, 304, 333, 418, 1289, 1886, 1989
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 6 cut(s) 229, 1512, 1972, 2524, 2631, 2751
BssMI GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
BssNI GRCGYC 1 cut(s) 2031
BssT1I CCWWGG 5 cut(s) 1512, 1972, 2524, 2631, 2751
Bst2UI CCWGG 3 cut(s) 231, 1475, 2329
Bst6I CTCTTC 1 cut(s) 969
BstACI GRCGYC 1 cut(s) 2031
BstAPI GCANNNNNTGC 2 cut(s) 1927, 1955
BstBAI YACGTR 1 cut(s) 1508
BstBI TTCGAA 1 cut(s) 3001
BstC8I GCNNGC 7 cut(s) 18, 951, 969, 1234, 2374, 2413, 2736
BstDSI CCRYGG 1 cut(s) 2524
BstEII GGTNACC 1 cut(s) 1984
BstENI CCTNNNNNAGG 1 cut(s) 2682
BstF5I GGATG 5 cut(s) 273, 886, 1108, 1282, 2281
BstH2I RGCGCY 1 cut(s) 706
BstHHI GCGC 2 cut(s) 705, 2320
BstKTI GATC 8 cut(s) 616, 1265, 1461, 2279, 2344, 2674, 2797, 2857
BstMAI GTCTC 5 cut(s) 126, 187, 1034, 1045, 1584
BstMBI GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
BstNI CCWGG 3 cut(s) 231, 1475, 2329
BstNSI RCATGY 2 cut(s) 2353, 2738
BstPI GGTNACC 1 cut(s) 1984
BstSCI CCNGG 3 cut(s) 229, 1473, 2327
BstSFI CTRYAG 5 cut(s) 327, 1462, 1755, 2496, 2679
BstSLI GKGCMC 1 cut(s) 1717
BstV1I GCAGC 3 cut(s) 1016, 2236, 2614
BstV2I GAAGAC 3 cut(s) 315, 390, 1816
BstX2I RGATCY 5 cut(s) 1262, 1458, 2276, 2671, 2794
BstXI CCANNNNNNTGG 2 cut(s) 1724, 1789
BstYI RGATCY 5 cut(s) 1262, 1458, 2276, 2671, 2794
Bsu36I CCTNAGG 2 cut(s) 1425, 1680
BsuI GTATCC 2 cut(s) 2470, 2813
BsuRI GGCC 9 cut(s) 28, 220, 228, 346, 1502, 1517, 1715, 1971, 2828
BtgI CCRYGG 1 cut(s) 2524
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 5 cut(s) 273, 886, 1108, 1282, 2281
BtsI GCAGTG 2 cut(s) 1729, 2767
BtsIMutI CAGTG 9 cut(s) 51, 340, 667, 1365, 1599, 1729, 1782, 1893, 2767
BveI ACCTGC 1 cut(s) 1762
Cac8I GCNNGC 7 cut(s) 18, 951, 969, 1234, 2374, 2413, 2736
CaiI CAGNNNCTG 2 cut(s) 413, 1043
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 2 cut(s) 705, 2320
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 9 cut(s) 227, 233, 344, 1175, 1349, 1713, 1714, 1727, 2560
CseI GACGC 1 cut(s) 309
CsiI ACCWGGT 1 cut(s) 2327
Csp6I GTAC 6 cut(s) 1111, 1307, 1751, 1767, 2112, 2748
CspAI ACCGGT 1 cut(s) 82
CviQI GTAC 6 cut(s) 1111, 1307, 1751, 1767, 2112, 2748
DpnI GATC 8 cut(s) 615, 1264, 1460, 2278, 2343, 2673, 2796, 2856
DpnII GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
DraI TTTAAA 1 cut(s) 1132
DraIII CACNNNGTG 1 cut(s) 1927
EaeI YGGCCR 1 cut(s) 2826
Eam1104I CTCTTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 969
Eco130I CCWWGG 5 cut(s) 1512, 1972, 2524, 2631, 2751
Eco147I AGGCCT 2 cut(s) 220, 1971
Eco24I GRGCYC 1 cut(s) 1717
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 1 cut(s) 2290
Eco47I GGWCC 5 cut(s) 233, 1175, 1349, 1727, 2560
Eco81I CCTNAGG 2 cut(s) 1425, 1680
Eco88I CYCGRG 1 cut(s) 1998
Eco91I GGTNACC 1 cut(s) 1984
EcoNI CCTNNNNNAGG 1 cut(s) 2682
EcoO109I RGGNCCY 2 cut(s) 1175, 2560
EcoO65I GGTNACC 1 cut(s) 1984
EcoRII CCWGG 3 cut(s) 229, 1473, 2327
EcoRV GATATC 1 cut(s) 2290
EcoT14I CCWWGG 5 cut(s) 1512, 1972, 2524, 2631, 2751
EcoT22I ATGCAT 2 cut(s) 1807, 2355
EcoT38I GRGCYC 1 cut(s) 1717
ErhI CCWWGG 5 cut(s) 1512, 1972, 2524, 2631, 2751
Esp3I CGTCTC 1 cut(s) 1045
FaqI GGGAC 3 cut(s) 1227, 1713, 2573
FauI CCCGC 1 cut(s) 2185
FauNDI CATATG 1 cut(s) 1807
FbaI TGATCA 1 cut(s) 613
FblI GTMKAC 3 cut(s) 1596, 2028, 2157
Fnu4HI GCNGC 3 cut(s) 1005, 2250, 2628
FokI GGATG 5 cut(s) 260, 893, 1115, 1289, 2268
FriOI GRGCYC 1 cut(s) 1717
Fsp4HI GCNGC 3 cut(s) 1005, 2250, 2628
FspBI CTAG 8 cut(s) 168, 186, 270, 378, 1122, 2255, 2313, 2927
GlaI GCGC 2 cut(s) 704, 2319
GluI GCNGC 3 cut(s) 1005, 2250, 2628
GsuI CTGGAG 2 cut(s) 1457, 2775
HaeII RGCGCY 1 cut(s) 706
HaeIII GGCC 9 cut(s) 28, 220, 228, 346, 1502, 1517, 1715, 1971, 2828
HapII CCGG 4 cut(s) 83, 1100, 1623, 3009
HgaI GACGC 1 cut(s) 309
HhaI GCGC 2 cut(s) 705, 2320
Hin1I GRCGYC 1 cut(s) 2031
Hin6I GCGC 2 cut(s) 703, 2318
HinP1I GCGC 2 cut(s) 703, 2318
HincII GTYRAC 2 cut(s) 1074, 2029
HindII GTYRAC 2 cut(s) 1074, 2029
HindIII AAGCTT 2 cut(s) 421, 489
HinfI GANTC 4 cut(s) 395, 1910, 2577, 2718
HpaII CCGG 4 cut(s) 83, 1100, 1623, 3009
HphI GGTGA 8 cut(s) 396, 616, 1159, 1292, 1905, 1988, 2012, 2864
Hpy166II GTNNAC 7 cut(s) 1074, 1597, 2029, 2158, 2451, 2765, 2954
Hpy8I GTNNAC 7 cut(s) 1074, 1597, 2029, 2158, 2451, 2765, 2954
Hpy99I CGWCG 1 cut(s) 2033
HpyAV CCTTC 8 cut(s) 841, 1543, 1560, 1835, 1900, 2138, 2609, 2943
HpyCH4IV ACGT 4 cut(s) 6, 529, 1507, 2031
HpySE526I ACGT 4 cut(s) 6, 529, 1507, 2031
Hsp92I GRCGYC 1 cut(s) 2031
HspAI GCGC 2 cut(s) 703, 2318
KflI GGGWCCC 1 cut(s) 2560
Kpn2I TCCGGA 1 cut(s) 1099
KpnI GGTACC 1 cut(s) 1754
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 8 cut(s) 686, 946, 1476, 1480, 1703, 2408, 2509, 2794
Lsp1109I GCAGC 3 cut(s) 1016, 2236, 2614
LweI GCATC 5 cut(s) 282, 871, 1685, 1792, 2209
MabI ACCWGGT 1 cut(s) 2327
MaeI CTAG 8 cut(s) 168, 186, 270, 378, 1122, 2255, 2313, 2927
MaeII ACGT 4 cut(s) 6, 529, 1507, 2031
MaeIII GTNAC 7 cut(s) 85, 402, 1280, 1633, 1911, 1984, 2323
MalI GATC 8 cut(s) 615, 1264, 1460, 2278, 2343, 2673, 2796, 2856
MbiI CCGCTC 2 cut(s) 1483, 1798
MboI GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 5 cut(s) 1262, 1458, 2276, 2671, 2794
MhlI GDGCHC 3 cut(s) 1717, 2413, 2863
MlsI TGGCCA 1 cut(s) 2828
MluNI TGGCCA 1 cut(s) 2828
MlyI GAGTC 2 cut(s) 404, 1919
MmeI TCCRAC 3 cut(s) 489, 1205, 1945
Mox20I TGGCCA 1 cut(s) 2828
Mph1103I ATGCAT 2 cut(s) 1807, 2355
MroI TCCGGA 1 cut(s) 1099
MroXI GAANNNNTTC 3 cut(s) 630, 851, 2098
MscI TGGCCA 1 cut(s) 2828
MseI TTAA 8 cut(s) 636, 693, 1131, 1490, 1704, 2015, 2073, 2702
MslI CAYNNNNRTG 5 cut(s) 1389, 1722, 1787, 1886, 2301
Msp20I TGGCCA 1 cut(s) 2828
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 4 cut(s) 83, 1100, 1623, 3009
MspR9I CCNGG 3 cut(s) 231, 1475, 2329
MunI CAATTG 1 cut(s) 1242
Mva1269I GAATGC 1 cut(s) 1366
MvaI CCWGG 3 cut(s) 231, 1475, 2329
NcoI CCATGG 1 cut(s) 2524
NdeI CATATG 1 cut(s) 1807
NdeII GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
NmeAIII GCCGAG 1 cut(s) 1021
NmuCI GTSAC 3 cut(s) 402, 1280, 1911
NsiI ATGCAT 2 cut(s) 1807, 2355
NspI RCATGY 2 cut(s) 2353, 2738
NspV TTCGAA 1 cut(s) 3001
OliI CACNNNNGTG 1 cut(s) 1722
PaeI GCATGC 1 cut(s) 2738
PagI TCATGA 1 cut(s) 1390
PceI AGGCCT 2 cut(s) 220, 1971
PciSI GCTCTTC 1 cut(s) 969
PctI GAATGC 1 cut(s) 1366
PdmI GAANNNNTTC 3 cut(s) 630, 851, 2098
PfeI GAWTC 2 cut(s) 2577, 2718
PflFI GACNNNGTC 1 cut(s) 1820
PflMI CCANNNNNTGG 2 cut(s) 413, 1892
PfoI TCCNGGA 1 cut(s) 1473
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 3 cut(s) 1006, 2251, 2629
PleI GAGTC 2 cut(s) 403, 1918
PpsI GAGTC 2 cut(s) 403, 1918
Ppu21I YACGTR 1 cut(s) 1508
PpuMI RGGWCCY 2 cut(s) 1175, 2560
PsiI TTATAA 1 cut(s) 1161
Psp5II RGGWCCY 2 cut(s) 1175, 2560
Psp6I CCWGG 3 cut(s) 229, 1473, 2327
PspEI GGTNACC 1 cut(s) 1984
PspGI CCWGG 3 cut(s) 229, 1473, 2327
PspOMI GGGCCC 1 cut(s) 1713
PspPI GGNCC 9 cut(s) 227, 233, 344, 1175, 1349, 1713, 1714, 1727, 2560
PspPPI RGGWCCY 2 cut(s) 1175, 2560
PsrI GAACNNNNNNTAC 2 cut(s) 1080, 1112
PstI CTGCAG 2 cut(s) 1759, 2500
PstNI CAGNNNCTG 2 cut(s) 413, 1043
PsuI RGATCY 5 cut(s) 1262, 1458, 2276, 2671, 2794
PsyI GACNNNGTC 1 cut(s) 1820
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 6 cut(s) 1112, 1308, 1752, 1768, 2113, 2749
RsaNI GTAC 6 cut(s) 1111, 1307, 1751, 1767, 2112, 2748
RseI CAYNNNNRTG 5 cut(s) 1389, 1722, 1787, 1886, 2301
SalI GTCGAC 1 cut(s) 2027
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 8 cut(s) 636, 693, 1131, 1490, 1704, 2015, 2073, 2702
SatI GCNGC 3 cut(s) 1005, 2250, 2628
Sau3AI GATC 8 cut(s) 613, 1262, 1458, 2276, 2341, 2671, 2794, 2854
Sau96I GGNCC 9 cut(s) 227, 233, 344, 1175, 1349, 1713, 1714, 1727, 2560
SchI GAGTC 2 cut(s) 404, 1919
ScrFI CCNGG 3 cut(s) 231, 1475, 2329
SduI GDGCHC 3 cut(s) 1717, 2413, 2863
SexAI ACCWGGT 1 cut(s) 2327
SfaNI GCATC 5 cut(s) 282, 871, 1685, 1792, 2209
SfcI CTRYAG 5 cut(s) 327, 1462, 1755, 2496, 2679
SfuI TTCGAA 1 cut(s) 3001
SinI GGWCC 5 cut(s) 233, 1175, 1349, 1727, 2560
SmiMI CAYNNNNRTG 5 cut(s) 1389, 1722, 1787, 1886, 2301
SmlI CTYRAG 4 cut(s) 568, 597, 908, 929
SmoI CTYRAG 4 cut(s) 568, 597, 908, 929
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 2738
SseBI AGGCCT 2 cut(s) 220, 1971
SsiI CCGC 6 cut(s) 1352, 1483, 1720, 1796, 2192, 2222
SspI AATATT 2 cut(s) 1436, 2940
SspMI CTAG 8 cut(s) 168, 186, 270, 378, 1122, 2255, 2313, 2927
StuI AGGCCT 2 cut(s) 220, 1971
StyD4I CCNGG 3 cut(s) 229, 1473, 2327
StyI CCWWGG 5 cut(s) 1512, 1972, 2524, 2631, 2751
TaiI ACGT 4 cut(s) 9, 532, 1510, 2034
TaqI TCGA 4 cut(s) 1800, 2028, 2698, 3001
TfiI GAWTC 2 cut(s) 2577, 2718
Tru1I TTAA 8 cut(s) 636, 693, 1131, 1490, 1704, 2015, 2073, 2702
Tru9I TTAA 8 cut(s) 636, 693, 1131, 1490, 1704, 2015, 2073, 2702
TscAI CASTG 9 cut(s) 51, 340, 667, 1372, 1606, 1729, 1789, 1893, 2767
TseFI GTSAC 3 cut(s) 402, 1280, 1911
TseI GCWGC 3 cut(s) 1004, 2249, 2627
Tsp45I GTSAC 3 cut(s) 402, 1280, 1911
TspGWI ACGGA 2 cut(s) 1686, 2553
TspRI CASTG 9 cut(s) 51, 340, 667, 1372, 1606, 1729, 1789, 1893, 2767
Tth111I GACNNNGTC 1 cut(s) 1820
Van91I CCANNNNNTGG 2 cut(s) 413, 1892
VpaK11BI GGWCC 5 cut(s) 233, 1175, 1349, 1727, 2560
XagI CCTNNNNNAGG 1 cut(s) 2682
XapI RAATTY 3 cut(s) 2078, 2402, 2821
XbaI TCTAGA 1 cut(s) 2254
XceI RCATGY 2 cut(s) 2353, 2738
XmiI GTMKAC 3 cut(s) 1596, 2028, 2157
XmnI GAANNNNTTC 3 cut(s) 630, 851, 2098
XspI CTAG 8 cut(s) 168, 186, 270, 378, 1122, 2255, 2313, 2927
ZraI GACGTC 1 cut(s) 2032
Zsp2I ATGCAT 2 cut(s) 1807, 2355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.