Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003232

Physical Location & Seq

Genomic Coordinates Forward (+)

40341761 .. 40345809

Gene Structure 4049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1321600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGAAGAGTTATATTCTTCATGTTTCAAAGGAAACCTGGCAAATGGGGTTTCATACCAAATTACTGCCAGTTCAGCCGATTTTTCAAGGAGGAGACAAAGAAAAGTGTCTCCAGGTTCAAAGGGATTAGTGCCGAAAAACCCAGTAGGAACTAGCAGCCAGAAGACGAATCAAAGGAATATTGGGGACGAAAAGGGTATTACATCTTCGACATCCAGTGAACTTGCTGGGAAAAATAAGAAAACACTTGAATCAAGAGTTGACACCAGAAGAGAATTGGTAGTTGAACCTTCAGAAGAGAGCGCTGTTGAGGAAAAAAGAATTGATGAAACTAGTAGTAAGGTTGAGGAATCATCTTCAGTTAGCAAGCCATCAGGTAGGGGGGGCATTCAAGGGATAGAAAATGGGAGTGTAGGTAAGGTCCTTGATGACTTGGCAGACACTGATAAAGGCATTGATACTAGTGAAAAGTTAACGAATGAAGTTTCTTTAAAGTTAAAGTTGGAGAGGGAAGAAAAATTACGTAAAGAGGAAATTGCGAGGCTTGCTGAGGAGAACTTCTCAAGCGGGTACAAAATCTTTGTCTATCCTCAGGTGGGGAAACCTGATCAAGACATAGAAGTGTTCCTTAATAGAAGTATATCAACCCTAAGCAATGAGCCAGAGGTTCAGATGATGGGTGCCTTTAACGACTGGCGTTGGAAATCTTTTACCTTCAGGTTGAACAAAACACAACTCAAAGGGGATTGGTGGTCTTGCCGGTTTCATGTTCCTAAAGAATCATATAAGATAAACTTTGTGTTCTTCAATGGGCAAAATGTTTATGACAATAATGATGCAAAAGATTTCTACATAACTGTGGAAGGTGGAATGGACCTCTTTGCATTTGAAGATTTCTTGCTTGAGGAGAAGCGTAAAGAACAAGAGAAACTTGCAAAGGAGCGAGCTGAGAGGGAAAGGCAAGCAGAAGAGCAGAGGCGAATTGAAGCAGAGAAGGCAGCAAGTGAAGCTGACAGAGCACAGGCAAAGGCAGAGATCGCAAAGAGACGACAAATGGTGCAAGAGTTGATCAAAAAGGCTGTAAGGTCTGTAGAAGACGTTTGGCACATAGAGCCTAGAGAGTTTAATGGTGAGGACTTGGTGAAGTTATACTATAACCGAAGCTCGGGTCCTCTTGCTCATGCTAAGGAACTATGGGTTCATGGGGGGCATAATGGTTGGATGGATGGATTGTCAATTGTTGAAAGACTTGTTAGCTCTGAGAGAAAGGATGGTGACTGGTGGTATGCCAATGTTGTTGTACCTGAACAAGCTGTTGTCTTGGATTGGGTTTTTGCTGATGGACCACCTCAGAATGCGGTTCTATATGATAACAACCACCGTCATGATTTCCATGCTATAGTTCCAAAATCCATTCCTGAGGAACTATATTGGGTTGAGGAAGAACACCAGATATATAAGAAGCTCCAGGAGGAGAGGAGATTAAAGGAGGAGGCAATACGTGCCAAGGCCGAAAGAACAGCTCGTATGAAAGCAGAAATGAAGGAAAGAATTTTGAAAAGGTTTTTGCTGTCTCAGAAGCATATAGTATACACCGAGCCTCTTGATGTTCAAGCTGGAAACAAGGCGACAGTTTTCTACCATCCTGCCAACACAGTTCTGAATGGAAAGCCTGAGGTTTGGTTCAGAGGATCATTTAACCGTTGGACCCACCACAAGGGTCCATTGCAATCACAGAAGATGTTACCCACAGAGAATGGTTCTCATGTCAAAACAACTGTTCCAGTCCCACTCGATGCATACATGATGGACTTTGTTTTCTCTGAGACGGAAGATGGTGGACTTTTTGACAACAATAATGGCATGGATTACCACATTCCGGTGTTTGGAGGAGTTGCGAAGGAGCCACCAATGAACATTGTACATATTGCTGTTGGAGGCCTTGGTGACGTTGTTACCAGTCTCTCACGAGCTGTGCAAGACTTAAATCACCACGTTGACATCATTCTTCCAAAGTATGATTGTTTGAACTTTAGCAATGTGAAGGAGTTTCAGTATAACAGAAGCTTCTCTTGGGGTGGAACTGAAATAAAAGTTTGGTTTGGGAAAGTGGAAGGCATTTCAGTGTACTTTTTGGAACCTCAAAACAGGTACTTTCCTCTTTCACGTCCTTCAAACCATGTGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0010021 GO:0042802 GO:0043170 GO:0071704 GO:1901135 GO:1901137 GO:1901576 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026

Pfam Domains

Protein Families

CBM53 Glyco_transf_5 Glycos_transf_1

Protein Analysis

730

Amino Acids

83.47

Weight (kDa)

6.17

Isoelectric Point (pI)

52.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM53	PF16760	207 - 266	5.8e-10	Starch/carbohydrate-binding module (family 53)
CBM53	PF16760	381 - 443	1.5e-14	Starch/carbohydrate-binding module (family 53)
CBM53	PF16760	544 - 605	2.5e-12	Starch/carbohydrate-binding module (family 53)
Glyco_transf_5	PF08323	643 - 719	3e-12	Starch synthase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11720 AT1G11720
fragaria_vesca	FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040 FvH4_3g02040
malus_domestica	MD05G1347400.v1.1 MD10G1321600.v1.1
prunus_persica	Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1 Prupe.4G020100_v2.0.a1
pyrus_communis	pycom05g31510 pycom10g27230
rosa_chinensis	RchiOBHm_Chr5g0002941
rosa_laevigata	RLG00000031072
rosa_multiflora	Rmu_sc0013603.1_g000004
rosa_roxburghii	Rroxscaffold_1G00072920
rosa_rugosa	Rorug04G0400700 Rorug04G0400800 Rorug04G0400800
rosa_samantha	Rh5AG023700 Rh5BG026500 Rh5CG026400 Rh5DG025000
rosa_wichuraiana	Rw5G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	680
AccI	GTMKAC	1 cut(s)	1590
AciI	CCGC	2 cut(s)	567, 1358
AclWI	GGATC	1 cut(s)	1699
AcsI	RAATTY	1 cut(s)	1551
AcuI	CTGAAG	3 cut(s)	276, 342, 700
AfaI	GTAC	5 cut(s)	572, 1302, 1923, 2129, 2153
AfeI	AGCGCT	1 cut(s)	304
AfiI	CCNNNNNNNGG	5 cut(s)	596, 1165, 1717, 1879, 1886
AhlI	ACTAGT	2 cut(s)	332, 461
AjiI	CACGTC	1 cut(s)	2168
AjnI	CCWGG	3 cut(s)	36, 112, 1467
AjuI	GAANNNNNNNTTGG	2 cut(s)	2056, 2088
Alw21I	GWGCWC	1 cut(s)	1021
Alw26I	GTCTC	6 cut(s)	88, 114, 1039, 1578, 1820, 1967
AlwI	GGATC	1 cut(s)	1699
AlwNI	CAGNNNCTG	1 cut(s)	443
Ama87I	CYCGRG	1 cut(s)	1165
Aor51HI	AGCGCT	1 cut(s)	304
AoxI	GGCC	2 cut(s)	1509, 1939
ApeKI	GCWGC	2 cut(s)	156, 998
ApoI	RAATTY	1 cut(s)	1551
ArsI	GACNNNNNNTTYG	4 cut(s)	567, 599, 1153, 1185
AspLEI	GCGC	1 cut(s)	305
AspS9I	GGNCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
AsuHPI	GGTGA	5 cut(s)	1142, 1153, 1286, 1958, 1982
AvaI	CYCGRG	1 cut(s)	1165
AvaII	GGWCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
AxyI	CCTNAGG	3 cut(s)	591, 1419, 1674
BanI	GGYRCC	1 cut(s)	680
BarI	GAAGNNNNNNTAC	4 cut(s)	190, 222, 504, 536
BauI	CACGAG	1 cut(s)	1968
BbsI	GAAGAC	2 cut(s)	170, 1101
Bbv12I	GWGCWC	1 cut(s)	1021
BbvCI	CCTCAGC	1 cut(s)	549
BbvI	GCAGC	2 cut(s)	168, 1010
BccI	CCATC	9 cut(s)	379, 670, 1216, 1220, 1265, 1334, 1650, 1801, 1829
BciT130I	CCWGG	3 cut(s)	38, 114, 1469
BclI	TGATCA	2 cut(s)	607, 1068
BcoDI	GTCTC	6 cut(s)	88, 114, 1039, 1578, 1820, 1967
BcuI	ACTAGT	2 cut(s)	332, 461
BfaI	CTAG	4 cut(s)	153, 333, 462, 1116
BfmI	CTRYAG	2 cut(s)	1089, 1398
BfoI	RGCGCY	1 cut(s)	306
BisI	GCNGC	2 cut(s)	157, 999
BlsI	GCNGC	2 cut(s)	158, 1000
Bme1390I	CCNGG	3 cut(s)	38, 114, 1469
Bme18I	GGWCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
BmeT110I	CYCGRG	1 cut(s)	1165
BmgBI	CACGTC	1 cut(s)	2168
BmgT120I	GGNCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
BmiI	GGNNCC	6 cut(s)	682, 1170, 1709, 1722, 1905, 2139
BmrFI	CCNGG	3 cut(s)	38, 114, 1469
BmrI	ACTGGG	1 cut(s)	137
BmsI	GCATC	2 cut(s)	826, 1786
BmuI	ACTGGG	1 cut(s)	137
BpiI	GAAGAC	2 cut(s)	170, 1101
BplI	GAGNNNNNCTC	2 cut(s)	545, 577
BpmI	CTGGAG	2 cut(s)	96, 1451
Bpu10I	CCTNAGC	3 cut(s)	549, 650, 1185
BpuEI	CTTGAG	2 cut(s)	547, 923
BsaAI	YACGTR	2 cut(s)	524, 1502
BsaJI	CCNNGG	2 cut(s)	1506, 1942
BsaWI	WCCGGW	1 cut(s)	1879
BsaXI	ACNNNNNCTCC	4 cut(s)	400, 430, 2039, 2069
Bsc4I	CCNNNNNNNGG	5 cut(s)	596, 1165, 1717, 1879, 1886
Bse118I	RCCGGY	1 cut(s)	759
Bse1I	ACTGG	7 cut(s)	69, 143, 216, 698, 1283, 1784, 1959
Bse21I	CCTNAGG	3 cut(s)	591, 1419, 1674
Bse3DI	GCAATG	3 cut(s)	661, 1724, 2044
BseBI	CCWGG	3 cut(s)	38, 114, 1469
BseDI	CCNNGG	2 cut(s)	1506, 1942
BseGI	GGATG	5 cut(s)	212, 1227, 1231, 1276, 1642
BseLI	CCNNNNNNNGG	5 cut(s)	596, 1165, 1717, 1879, 1886
BseMI	GCAATG	3 cut(s)	661, 1724, 2044
BseMII	CTCAG	9 cut(s)	540, 605, 939, 1251, 1364, 1410, 1589, 1665, 1815
BseNI	ACTGG	7 cut(s)	69, 143, 216, 698, 1283, 1784, 1959
BseRI	GAGGAG	7 cut(s)	106, 566, 920, 1487, 1492, 1505, 1905
BseXI	GCAGC	2 cut(s)	168, 1010
BseYI	CCCAGC	1 cut(s)	227
BshFI	GGCC	2 cut(s)	1511, 1941
BshNI	GGYRCC	1 cut(s)	680
BsiHKAI	GWGCWC	1 cut(s)	1021
BsiHKCI	CYCGRG	1 cut(s)	1165
BsiSI	CCGG	2 cut(s)	760, 1880
BslFI	GGGAC	2 cut(s)	200, 1772
BslI	CCNNNNNNNGG	5 cut(s)	596, 1165, 1717, 1879, 1886
BsmAI	GTCTC	6 cut(s)	88, 114, 1039, 1578, 1820, 1967
BsmBI	CGTCTC	2 cut(s)	1039, 1820
BsmFI	GGGAC	2 cut(s)	200, 1772
BsmI	GAATGC	2 cut(s)	387, 1360
BsnI	GGCC	2 cut(s)	1511, 1941
BsoBI	CYCGRG	1 cut(s)	1165
Bsp1286I	GDGCHC	1 cut(s)	1021
Bsp1407I	TGTACA	1 cut(s)	1921
Bsp143I	GATC	4 cut(s)	607, 1035, 1068, 1691
BspACI	CCGC	2 cut(s)	567, 1358
BspANI	GGCC	2 cut(s)	1511, 1941
BspCNI	CTCAG	9 cut(s)	541, 604, 940, 1252, 1363, 1411, 1588, 1666, 1816
BspHI	TCATGA	1 cut(s)	1384
BspLI	GGNNCC	6 cut(s)	682, 1170, 1709, 1722, 1905, 2139
BspPI	GGATC	1 cut(s)	1699
BspQI	GCTCTTC	1 cut(s)	963
BspT107I	GGYRCC	1 cut(s)	680
BsrDI	GCAATG	3 cut(s)	661, 1724, 2044
BsrFI	RCCGGY	1 cut(s)	759
BsrGI	TGTACA	1 cut(s)	1921
BsrI	ACTGG	7 cut(s)	69, 143, 216, 698, 1283, 1784, 1959
BssAI	RCCGGY	1 cut(s)	759
BssECI	CCNNGG	2 cut(s)	1506, 1942
BssMI	GATC	4 cut(s)	607, 1035, 1068, 1691
BssNAI	GTATAC	1 cut(s)	1591
BssSI	CACGAG	1 cut(s)	1968
BssT1I	CCWWGG	2 cut(s)	1506, 1942
Bst1107I	GTATAC	1 cut(s)	1591
Bst2BI	CACGAG	1 cut(s)	1968
Bst2UI	CCWGG	3 cut(s)	38, 114, 1469
Bst4CI	ACNGT	6 cut(s)	859, 1382, 1633, 1657, 1703, 1780
Bst6I	CTCTTC	3 cut(s)	265, 291, 963
BstAPI	GCANNNNNTGC	1 cut(s)	1502
BstAUI	TGTACA	1 cut(s)	1921
BstBAI	YACGTR	2 cut(s)	524, 1502
BstC8I	GCNNGC	4 cut(s)	368, 546, 945, 963
BstF5I	GGATG	5 cut(s)	212, 1227, 1231, 1276, 1642
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	1 cut(s)	305
BstKTI	GATC	4 cut(s)	610, 1038, 1071, 1694
BstMAI	GTCTC	6 cut(s)	88, 114, 1039, 1578, 1820, 1967
BstMBI	GATC	4 cut(s)	607, 1035, 1068, 1691
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 545, 995, 1007, 1016, 1037, 1111, 1502
BstNI	CCWGG	3 cut(s)	38, 114, 1469
BstSCI	CCNGG	3 cut(s)	36, 112, 1467
BstSFI	CTRYAG	2 cut(s)	1089, 1398
BstSNI	TACGTA	1 cut(s)	524
BstV1I	GCAGC	2 cut(s)	168, 1010
BstV2I	GAAGAC	2 cut(s)	170, 1101
BstZ17I	GTATAC	1 cut(s)	1591
Bsu36I	CCTNAGG	3 cut(s)	591, 1419, 1674
BsuRI	GGCC	2 cut(s)	1511, 1941
BtrI	CACGTC	1 cut(s)	2168
BtsCI	GGATG	5 cut(s)	212, 1227, 1231, 1276, 1642
BtsIMutI	CAGTG	3 cut(s)	223, 441, 2130
Cac8I	GCNNGC	4 cut(s)	368, 546, 945, 963
CaiI	CAGNNNCTG	1 cut(s)	443
CciI	TCATGA	1 cut(s)	1384
CfoI	GCGC	1 cut(s)	305
Cfr10I	RCCGGY	1 cut(s)	759
Cfr13I	GGNCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
Csp6I	GTAC	5 cut(s)	571, 1301, 1922, 2128, 2152
CviQI	GTAC	5 cut(s)	571, 1301, 1922, 2128, 2152
DpnI	GATC	4 cut(s)	609, 1037, 1070, 1693
DpnII	GATC	4 cut(s)	607, 1035, 1068, 1691
DraI	TTTAAA	1 cut(s)	492
Eam1104I	CTCTTC	3 cut(s)	265, 291, 963
EarI	CTCTTC	3 cut(s)	265, 291, 963
Eco105I	TACGTA	1 cut(s)	524
Eco130I	CCWWGG	2 cut(s)	1506, 1942
Eco147I	AGGCCT	1 cut(s)	1941
Eco47I	GGWCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
Eco47III	AGCGCT	1 cut(s)	304
Eco57I	CTGAAG	3 cut(s)	276, 342, 700
Eco81I	CCTNAGG	3 cut(s)	591, 1419, 1674
Eco88I	CYCGRG	1 cut(s)	1165
EcoO109I	RGGNCCY	2 cut(s)	421, 1169
EcoRII	CCWGG	3 cut(s)	36, 112, 1467
EcoT14I	CCWWGG	2 cut(s)	1506, 1942
EcoT22I	ATGCAT	1 cut(s)	1801
ErhI	CCWWGG	2 cut(s)	1506, 1942
Esp3I	CGTCTC	2 cut(s)	1039, 1820
FalI	AAGNNNNNCTT	8 cut(s)	612, 644, 779, 811, 915, 947, 2056, 2088
FaqI	GGGAC	2 cut(s)	200, 1772
FauI	CCCGC	1 cut(s)	560
FbaI	TGATCA	2 cut(s)	607, 1068
FblI	GTMKAC	1 cut(s)	1590
Fnu4HI	GCNGC	2 cut(s)	157, 999
FokI	GGATG	5 cut(s)	199, 1234, 1238, 1283, 1629
Fsp4HI	GCNGC	2 cut(s)	157, 999
FspBI	CTAG	4 cut(s)	153, 333, 462, 1116
GlaI	GCGC	1 cut(s)	304
GluI	GCNGC	2 cut(s)	157, 999
GsaI	CCCAGC	1 cut(s)	231
GsuI	CTGGAG	2 cut(s)	96, 1451
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	2 cut(s)	1511, 1941
HapII	CCGG	2 cut(s)	760, 1880
HhaI	GCGC	1 cut(s)	305
Hin6I	GCGC	1 cut(s)	303
HinP1I	GCGC	1 cut(s)	303
HincII	GTYRAC	3 cut(s)	262, 474, 1999
HindII	GTYRAC	3 cut(s)	262, 474, 1999
HindIII	AAGCTT	1 cut(s)	2065
HinfI	GANTC	4 cut(s)	169, 251, 350, 779
HpaI	GTTAAC	1 cut(s)	474
HpaII	CCGG	2 cut(s)	760, 1880
HphI	GGTGA	5 cut(s)	1142, 1153, 1286, 1958, 1982
Hpy166II	GTNNAC	7 cut(s)	221, 262, 474, 1591, 1841, 1999, 2128
Hpy188I	TCNGA	8 cut(s)	295, 672, 1261, 1353, 1578, 1662, 1688, 1825
Hpy188III	TCNNGA	6 cut(s)	255, 611, 1385, 1418, 1604, 1968
Hpy8I	GTNNAC	7 cut(s)	221, 262, 474, 1591, 1841, 1999, 2128
HpyAV	CCTTC	9 cut(s)	300, 724, 857, 988, 1537, 1894, 2038, 2108, 2181
HpyCH4III	ACNGT	6 cut(s)	859, 1382, 1633, 1657, 1703, 1780
HpyCH4IV	ACGT	6 cut(s)	523, 1098, 1501, 1950, 1995, 2167
HpyCH4V	TGCA	7 cut(s)	839, 884, 935, 1060, 1729, 1799, 1978
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 545, 995, 1007, 1016, 1037, 1111, 1502
HpySE526I	ACGT	6 cut(s)	523, 1098, 1501, 1950, 1995, 2167
HspAI	GCGC	1 cut(s)	303
Ksp22I	TGATCA	2 cut(s)	607, 1068
KspAI	GTTAAC	1 cut(s)	474
Kzo9I	GATC	4 cut(s)	607, 1035, 1068, 1691
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	3 cut(s)	940, 1470, 1903
Lsp1109I	GCAGC	2 cut(s)	168, 1010
LweI	GCATC	2 cut(s)	826, 1786
MaeI	CTAG	4 cut(s)	153, 333, 462, 1116
MaeII	ACGT	6 cut(s)	523, 1098, 1501, 1950, 1995, 2167
MaeIII	GTNAC	4 cut(s)	1274, 1743, 1946, 1954
MalI	GATC	4 cut(s)	609, 1037, 1070, 1693
MboI	GATC	4 cut(s)	607, 1035, 1068, 1691
MfeI	CAATTG	1 cut(s)	1236
MhlI	GDGCHC	1 cut(s)	1021
MluCI	AATT	8 cut(s)	60, 275, 321, 518, 534, 981, 1236, 1551
MmeI	TCCRAC	5 cut(s)	483, 680, 1199, 1685, 1915
Mph1103I	ATGCAT	1 cut(s)	1801
MseI	TTAA	9 cut(s)	473, 491, 497, 630, 687, 1125, 1484, 1698, 1985
MslI	CAYNNNNRTG	5 cut(s)	620, 857, 1383, 1880, 2123
MspI	CCGG	2 cut(s)	760, 1880
MspR9I	CCNGG	3 cut(s)	38, 114, 1469
MunI	CAATTG	1 cut(s)	1236
Mva1269I	GAATGC	2 cut(s)	387, 1360
MvaI	CCWGG	3 cut(s)	38, 114, 1469
MwoI	GCNNNNNNNGC	8 cut(s)	74, 545, 995, 1007, 1016, 1037, 1111, 1502
NdeII	GATC	4 cut(s)	607, 1035, 1068, 1691
NlaIV	GGNNCC	6 cut(s)	682, 1170, 1709, 1722, 1905, 2139
NmuCI	GTSAC	2 cut(s)	1274, 1946
NsiI	ATGCAT	1 cut(s)	1801
PagI	TCATGA	1 cut(s)	1384
PceI	AGGCCT	1 cut(s)	1941
PciSI	GCTCTTC	1 cut(s)	963
PctI	GAATGC	2 cut(s)	387, 1360
PfeI	GAWTC	4 cut(s)	169, 251, 350, 779
PfoI	TCCNGGA	1 cut(s)	1467
PkrI	GCNGC	2 cut(s)	158, 1000
Ppu21I	YACGTR	2 cut(s)	524, 1502
PpuMI	RGGWCCY	2 cut(s)	421, 1169
Psp5II	RGGWCCY	2 cut(s)	421, 1169
Psp6I	CCWGG	3 cut(s)	36, 112, 1467
PspFI	CCCAGC	1 cut(s)	227
PspGI	CCWGG	3 cut(s)	36, 112, 1467
PspN4I	GGNNCC	6 cut(s)	682, 1170, 1709, 1722, 1905, 2139
PspPI	GGNCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
PspPPI	RGGWCCY	2 cut(s)	421, 1169
PstNI	CAGNNNCTG	1 cut(s)	443
RsaI	GTAC	5 cut(s)	572, 1302, 1923, 2129, 2153
RsaNI	GTAC	5 cut(s)	571, 1301, 1922, 2128, 2152
RseI	CAYNNNNRTG	5 cut(s)	620, 857, 1383, 1880, 2123
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	9 cut(s)	473, 491, 497, 630, 687, 1125, 1484, 1698, 1985
SatI	GCNGC	2 cut(s)	157, 999
Sau3AI	GATC	4 cut(s)	607, 1035, 1068, 1691
Sau96I	GGNCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
ScrFI	CCNGG	3 cut(s)	38, 114, 1469
SduI	GDGCHC	1 cut(s)	1021
SfaNI	GCATC	2 cut(s)	826, 1786
SfcI	CTRYAG	2 cut(s)	1089, 1398
SinI	GGWCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
SmiMI	CAYNNNNRTG	5 cut(s)	620, 857, 1383, 1880, 2123
SmlI	CTYRAG	2 cut(s)	562, 902
SmoI	CTYRAG	2 cut(s)	562, 902
SnaBI	TACGTA	1 cut(s)	524
SpeI	ACTAGT	2 cut(s)	332, 461
Sse9I	AATT	8 cut(s)	60, 275, 321, 518, 534, 981, 1236, 1551
SseBI	AGGCCT	1 cut(s)	1941
SsiI	CCGC	2 cut(s)	567, 1358
SspI	AATATT	1 cut(s)	181
SspMI	CTAG	4 cut(s)	153, 333, 462, 1116
StuI	AGGCCT	1 cut(s)	1941
StyD4I	CCNGG	3 cut(s)	36, 112, 1467
StyI	CCWWGG	2 cut(s)	1506, 1942
TaaI	ACNGT	6 cut(s)	859, 1382, 1633, 1657, 1703, 1780
TaiI	ACGT	6 cut(s)	526, 1101, 1504, 1953, 1998, 2170
TaqI	TCGA	2 cut(s)	209, 1794
TasI	AATT	8 cut(s)	60, 275, 321, 518, 534, 981, 1236, 1551
TatI	WGTACW	2 cut(s)	1921, 2127
TfiI	GAWTC	4 cut(s)	169, 251, 350, 779
Tru1I	TTAA	9 cut(s)	473, 491, 497, 630, 687, 1125, 1484, 1698, 1985
Tru9I	TTAA	9 cut(s)	473, 491, 497, 630, 687, 1125, 1484, 1698, 1985
TscAI	CASTG	3 cut(s)	223, 448, 2130
TseFI	GTSAC	2 cut(s)	1274, 1946
TseI	GCWGC	2 cut(s)	156, 998
Tsp45I	GTSAC	2 cut(s)	1274, 1946
TspDTI	ATGAA	9 cut(s)	9, 42, 342, 495, 755, 1190, 1544, 1556, 1928
TspGWI	ACGGA	1 cut(s)	1844
TspRI	CASTG	3 cut(s)	223, 448, 2130
VpaK11BI	GGWCC	6 cut(s)	421, 874, 1169, 1343, 1707, 1721
XapI	RAATTY	1 cut(s)	1551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	274
XmiI	GTMKAC	1 cut(s)	1590
XspI	CTAG	4 cut(s)	153, 333, 462, 1116
Zsp2I	ATGCAT	1 cut(s)	1801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.