AT1G12410

Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010311
Physical Location & Seq
Forward (+)
4223030 .. 4225254
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12410.1

Sequence Viewer

Length: 840 bp
ATGGCGGTCTCGTTTAATACAACGCTTCACCAGCCTTCTCTGAGTCCGAGCTGTAGCATCAAGCTTTATTCTGGGTTAAAGCCTCAATCTGCAAGCTTTTTGGCAAGTGGGTATCAGAATTTGAACAAGGAGTTCTATGGAAGAGTTTATAAGAGTCTGCAATCTGGGACTGGGAAAGCGAGCAGGTCACGGGTAAAGATGATGCCAATAGGAACACCGAGAGTACCCTACAGAAACCGAGAAGAAGGGACTTGGCAATGGGTTGATATATGGAATGCCCTTTATCGAGAGCGTGTAATCTTCATTGGACAAAACATTGATGAAGAGTTTAGCAACCAGATATTAGCAACCATGTTGTACCTTGATACTCTTGATGACTCGAGGAGGATTTATATGTACCTAAATGGTCCGGGTGGTGATCTTACTCCAAGTCTAGCCATCTATGATACAATGAAGAGCTTGAAAAGTCCGGTTGGGACACATTGCGTTGGGCTTGCTTACAACCTTGCAGGTTTTCTTCTTGCGGCTGGAGAAAAGGGTCACCGATTTGCGATGCCATTATCAAGAATCGCCCTCCAGTCACCAGCTGGTGCAGCCCGTGGTCAGGCTGATGATATCCAAAATGAAGCAAAAGAGCTTTCAAGGATAAGAGACTACCTCTTCAATGAACTAGCCAAGAATACAGGCCAGCCTGCGGAAAGGGTCTTCAAAGACTTGAGCCGGGTGAAAAGGTTCAATGCAGAGGAAGCAATCGAGTATGGACTTATTGATAAGATTGTTAGACCACCGCGCATCAAAGAAGACGCTCCTCGCCAAGACGAAAGCGCAGGGCTAGGCTAG

Protein Analysis

279

Amino Acids

31.21

Weight (kDa)

9.19

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 86 - 261 2.7e-63 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
Acc36I ACCTGC 2 cut(s) 174, 500
AccII CGCG 1 cut(s) 790
AciI CCGC 4 cut(s) 5, 524, 695, 788
AcsI RAATTY 1 cut(s) 118
AfaI GTAC 3 cut(s) 225, 359, 398
AfiI CCNNNNNNNGG 2 cut(s) 604, 694
AgsI TTSAA 6 cut(s) 124, 463, 642, 664, 709, 736
AluBI AGCT 6 cut(s) 51, 64, 96, 459, 587, 637
AluI AGCT 6 cut(s) 51, 64, 96, 459, 587, 637
Alw26I GTCTC 2 cut(s) 13, 645
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 1 cut(s) 685
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 118
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 2 cut(s) 792, 827
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 411, 722
AsuHPI GGTGA 5 cut(s) 20, 428, 533, 573, 736
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 697, 807
BbvI GCAGC 1 cut(s) 605
BccI CCATC 1 cut(s) 446
BcnI CCSGG 2 cut(s) 411, 722
BcoDI GTCTC 2 cut(s) 13, 645
BfaI CTAG 4 cut(s) 434, 671, 833, 838
BfmI CTRYAG 2 cut(s) 52, 229
BfuAI ACCTGC 2 cut(s) 174, 500
BisI GCNGC 2 cut(s) 525, 594
BlsI GCNGC 2 cut(s) 526, 595
Bme1390I CCNGG 2 cut(s) 411, 722
Bme18I GGWCC 1 cut(s) 407
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 407
BmrFI CCNGG 2 cut(s) 411, 722
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 4 cut(s) 66, 192, 543, 801
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 697, 807
BplI GAGNNNNNCTC 2 cut(s) 642, 674
BpmI CTGGAG 2 cut(s) 549, 560
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 411, 722
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 598
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 694
Bse1I ACTGG 2 cut(s) 175, 577
Bse3DI GCAATG 2 cut(s) 263, 481
BseDI CCNNGG 1 cut(s) 598
BseLI CCNNNNNNNGG 2 cut(s) 604, 694
BseMI GCAATG 2 cut(s) 263, 481
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 2 cut(s) 175, 577
BseRI GAGGAG 2 cut(s) 397, 798
BseXI GCAGC 1 cut(s) 605
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 790
BshFI GGCC 1 cut(s) 687
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 3 cut(s) 410, 470, 721
BslFI GGGAC 3 cut(s) 181, 262, 490
BslI CCNNNNNNNGG 2 cut(s) 604, 694
BsmAI GTCTC 2 cut(s) 13, 645
BsmFI GGGAC 3 cut(s) 181, 262, 490
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 1 cut(s) 687
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 379
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 4 cut(s) 5, 524, 695, 788
BspANI GGCC 1 cut(s) 687
BspCNI CTCAG 1 cut(s) 33
BspFNI CGCG 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 174, 500
BspQI GCTCTTC 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 13
BsrDI GCAATG 2 cut(s) 263, 481
BsrI ACTGG 2 cut(s) 175, 577
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 1 cut(s) 418
Bst6I CTCTTC 4 cut(s) 136, 318, 449, 665
BstC8I GCNNGC 5 cut(s) 94, 181, 495, 689, 693
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 598
BstEII GGTNACC 1 cut(s) 539
BstFNI CGCG 1 cut(s) 790
BstHHI GCGC 2 cut(s) 792, 827
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 2 cut(s) 13, 645
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 3 cut(s) 31, 593, 746
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 409, 720
BstSFI CTRYAG 2 cut(s) 52, 229
BstUI CGCG 1 cut(s) 790
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 2 cut(s) 697, 807
BsuRI GGCC 1 cut(s) 687
BtgI CCRYGG 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 566
BveI ACCTGC 2 cut(s) 174, 500
Cac8I GCNNGC 5 cut(s) 94, 181, 495, 689, 693
CfoI GCGC 2 cut(s) 792, 827
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 1 cut(s) 812
Csp6I GTAC 3 cut(s) 224, 358, 397
CviAII CATG 1 cut(s) 352
CviQI GTAC 3 cut(s) 224, 358, 397
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 4 cut(s) 136, 318, 449, 665
EarI CTCTTC 4 cut(s) 136, 318, 449, 665
Eco31I GGTCTC 1 cut(s) 13
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 379
Eco91I GGTNACC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 539
EcoRV GATATC 1 cut(s) 616
FaeI CATG 1 cut(s) 355
FaiI YATR 9 cut(s) 138, 150, 269, 271, 353, 393, 395, 444, 759
FaqI GGGAC 3 cut(s) 181, 262, 490
FatI CATG 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 525, 594
Fsp4HI GCNGC 2 cut(s) 525, 594
FspBI CTAG 4 cut(s) 434, 671, 833, 838
GlaI GCGC 2 cut(s) 791, 826
GluI GCNGC 2 cut(s) 525, 594
GsuI CTGGAG 2 cut(s) 549, 560
HaeIII GGCC 1 cut(s) 687
HapII CCGG 3 cut(s) 410, 470, 721
HgaI GACGC 1 cut(s) 812
HhaI GCGC 2 cut(s) 792, 827
Hin1II CATG 1 cut(s) 355
Hin6I GCGC 2 cut(s) 790, 825
HinP1I GCGC 2 cut(s) 790, 825
HindIII AAGCTT 2 cut(s) 62, 94
HinfI GANTC 4 cut(s) 43, 154, 377, 567
HpaII CCGG 3 cut(s) 410, 470, 721
HphI GGTGA 5 cut(s) 20, 428, 533, 573, 736
Hpy188I TCNGA 3 cut(s) 42, 48, 117
Hpy188III TCNNGA 3 cut(s) 287, 371, 564
HpyAV CCTTC 2 cut(s) 45, 239
HpyCH4V TGCA 5 cut(s) 92, 160, 509, 593, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 593, 746
HpyF3I CTNAG 1 cut(s) 41
Hsp92II CATG 1 cut(s) 355
HspAI GCGC 2 cut(s) 790, 825
Kzo9I GATC 1 cut(s) 418
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 4 cut(s) 66, 192, 543, 801
MaeI CTAG 4 cut(s) 434, 671, 833, 838
MaeIII GTNAC 3 cut(s) 186, 539, 579
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 9 cut(s) 153, 254, 292, 335, 466, 509, 652, 697, 812
MluCI AATT 1 cut(s) 118
MlyI GAGTC 3 cut(s) 52, 163, 371
MnlI CCTC 7 cut(s) 93, 375, 378, 584, 668, 736, 819
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 2 cut(s) 15, 77
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 3 cut(s) 410, 470, 721
MspR9I CCNGG 2 cut(s) 411, 722
Mva1269I GAATGC 1 cut(s) 280
MvnI CGCG 1 cut(s) 790
MwoI GCNNNNNNNGC 3 cut(s) 31, 593, 746
NciI CCSGG 2 cut(s) 411, 722
NdeII GATC 1 cut(s) 418
NlaIII CATG 1 cut(s) 355
NmuCI GTSAC 3 cut(s) 186, 539, 579
PaeR7I CTCGAG 1 cut(s) 379
PciSI GCTCTTC 1 cut(s) 449
PctI GAATGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 1 cut(s) 567
PkrI GCNGC 2 cut(s) 526, 595
PleI GAGTC 3 cut(s) 51, 162, 371
PpsI GAGTC 3 cut(s) 51, 162, 371
PsiI TTATAA 1 cut(s) 150
PspEI GGTNACC 1 cut(s) 539
PspPI GGNCC 1 cut(s) 407
PspXI VCTCGAGB 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 3 cut(s) 225, 359, 398
RsaNI GTAC 3 cut(s) 224, 358, 397
SapI GCTCTTC 1 cut(s) 449
SaqAI TTAA 2 cut(s) 15, 77
SatI GCNGC 2 cut(s) 525, 594
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 3 cut(s) 52, 163, 371
ScrFI CCNGG 2 cut(s) 411, 722
SfaNI GCATC 4 cut(s) 66, 192, 543, 801
SfcI CTRYAG 2 cut(s) 52, 229
Sfr274I CTCGAG 1 cut(s) 379
SinI GGWCC 1 cut(s) 407
SlaI CTCGAG 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 379, 715
SmoI CTYRAG 2 cut(s) 379, 715
Sse9I AATT 1 cut(s) 118
SsiI CCGC 4 cut(s) 5, 524, 695, 788
SspMI CTAG 4 cut(s) 434, 671, 833, 838
StyD4I CCNGG 2 cut(s) 409, 720
TaqI TCGA 3 cut(s) 286, 380, 753
TasI AATT 1 cut(s) 118
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 2 cut(s) 15, 77
Tru9I TTAA 2 cut(s) 15, 77
TseFI GTSAC 3 cut(s) 186, 539, 579
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 3 cut(s) 186, 539, 579
TspDTI ATGAA 5 cut(s) 292, 336, 467, 639, 681
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XhoI CTCGAG 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 4 cut(s) 434, 671, 833, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.