FvH4_5g00850

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010311
Physical Location & Seq
Forward (+)
477589 .. 481202
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00850.t3

Sequence Viewer

Length: 864 bp
ATGGCAGTCTCTTTTGCCACGACGACGTACACTCCAAGCCTTCAGCCCCAAGCTAGACTCTCTCCCAGTTGCTCGATAAAGCATTTTTCTGGGTTAAAGCCTGAATCTTTAGGTACTTTTGGAGCTAAAAATCCAAACTTGACGGTTGAGTTATACAGCAAAGTTAGTAAGAGCCTTCTGCCCAGGACAGGAAACCACAGATCATCACGTGCACAAGTTCGAATGATGCCAATAGGGACACCAAAAGTTCCCTATCGAACTCCTGGTGAGGGAGGTTGGCAATGGGTTGACCTGTGGAATGCTCTGTACCGGGAACGTGTTATCTTCATTGGGCAAAATATAGATGAAGAGTTTAGCAATCAAATTCTGGCAACAATGCTATACCTCGACACTATTGATTCGTCCAAAAGGCTGTATATGTATATCAATGGACCTGGTGGAGATCTTACTCCGAGCATGGCTATTTATGATACTATGCAAAGCATACAGAGTCCTGTTGGCACTCACTGTGTGGGCTATGCCTATAATTTGGCAGCCTTTCTTCTTGCAGCTGGTGAAAAGGGTAATCGATTTGCAATGCCACTATCAAGAATTGCACTGCAGTCTCCTGCTGGGGCCGCTCGTGGTCAGGCTGATGACATACGCAATGAAGCAAATGAGCTTCTCAGAATCAGAGACTACCTTTTTAATGAGTTGGCAGAGAAAACCGGCCAACCAATTGAAAAGATTAACAAAGACCTAAGTCGGATGAAGCGGTTTAATGCACAGGAGGCTCTTGAGTATGGGCTCATTGATCGTGTAGTAAGGCCGCCTCGTATTAAGGCCGACTCACCTAGCAGAGAAGCGGGAACAGGTCTTGGTTAG

Protein Analysis

288

Amino Acids

31.79

Weight (kDa)

9.35

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 19 - 195 2.2e-65 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 620
AciI CCGC 4 cut(s) 618, 754, 809, 845
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 26
AcvI CACGTG 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 3 cut(s) 29, 115, 308
AfiI CCNNNNNNNGG 2 cut(s) 188, 269
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 1 cut(s) 722
AjnI CCWGG 3 cut(s) 182, 262, 433
AluBI AGCT 4 cut(s) 53, 125, 551, 661
AluI AGCT 4 cut(s) 53, 125, 551, 661
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 3 cut(s) 13, 609, 669
Alw44I GTGCAC 1 cut(s) 210
AoxI GGCC 4 cut(s) 615, 709, 806, 822
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 533, 548
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 431, 615
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 278, 566, 822
AsuII TTCGAA 1 cut(s) 220
AvaII GGWCC 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 214
BanII GRGCYC 1 cut(s) 789
BauI CACGAG 1 cut(s) 621
BbrPI CACGTG 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 545, 560
BciT130I CCWGG 3 cut(s) 184, 264, 435
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 3 cut(s) 13, 609, 669
BfaI CTAG 2 cut(s) 54, 834
BfmI CTRYAG 1 cut(s) 599
BglII AGATCT 1 cut(s) 442
BisI GCNGC 4 cut(s) 534, 549, 618, 809
BlsI GCNGC 4 cut(s) 535, 550, 619, 810
Bme1390I CCNGG 4 cut(s) 184, 264, 311, 435
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 431, 615
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 4 cut(s) 184, 264, 311, 435
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 60
BoxI GACNNNNGTC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 797
BpuMI CCSGG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 568
BsaAI YACGTR 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 269
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 3 cut(s) 287, 582, 652
BseBI CCWGG 3 cut(s) 184, 264, 435
BseCI ATCGAT 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 188, 269
BseMI GCAATG 3 cut(s) 287, 582, 652
BseMII CTCAG 1 cut(s) 679
BseNI ACTGG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 2 cut(s) 545, 560
BseYI CCCAGC 1 cut(s) 611
BshFI GGCC 4 cut(s) 617, 711, 808, 824
BshVI ATCGAT 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 310, 708
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 188, 269
BsmAI GTCTC 3 cut(s) 13, 609, 669
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 4 cut(s) 617, 711, 808, 824
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 2 cut(s) 214, 789
Bsp143I GATC 3 cut(s) 200, 442, 793
BspACI CCGC 4 cut(s) 618, 754, 809, 845
BspANI GGCC 4 cut(s) 617, 711, 808, 824
BspCNI CTCAG 1 cut(s) 678
BspDI ATCGAT 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 1 cut(s) 603
BspT104I TTCGAA 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 620
BsrDI GCAATG 3 cut(s) 287, 582, 652
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 3 cut(s) 200, 442, 793
BssSI CACGAG 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 3 cut(s) 184, 264, 435
Bst4CI ACNGT 2 cut(s) 145, 509
Bst6I CTCTTC 1 cut(s) 342
BstBAI YACGTR 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 665, 740
BstF5I GGATG 1 cut(s) 753
BstKTI GATC 3 cut(s) 203, 445, 796
BstMAI GTCTC 3 cut(s) 13, 609, 669
BstMBI GATC 3 cut(s) 200, 442, 793
BstMWI GCNNNNNNNGC 2 cut(s) 617, 770
BstNI CCWGG 3 cut(s) 184, 264, 435
BstPAI GACNNNNGTC 1 cut(s) 741
BstSCI CCNGG 4 cut(s) 182, 262, 309, 433
BstSFI CTRYAG 1 cut(s) 599
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 545, 560
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 568
BsuRI GGCC 4 cut(s) 617, 711, 808, 824
BsuTUI ATCGAT 1 cut(s) 568
BtsCI GGATG 1 cut(s) 753
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 2 cut(s) 505, 596
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 2 cut(s) 431, 615
ClaI ATCGAT 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 433
Csp6I GTAC 3 cut(s) 28, 114, 307
CviAII CATG 1 cut(s) 457
CviQI GTAC 3 cut(s) 28, 114, 307
DdeI CTNAG 2 cut(s) 665, 740
DpnI GATC 3 cut(s) 202, 444, 795
DpnII GATC 3 cut(s) 200, 442, 793
DraIII CACNNNGTG 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 789
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 26
Eco72I CACGTG 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 182, 262, 433
EcoT38I GRGCYC 1 cut(s) 789
FaeI CATG 1 cut(s) 460
FaqI GGGAC 1 cut(s) 250
FatI CATG 1 cut(s) 456
FauI CCCGC 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 534, 549, 618, 809
FokI GGATG 1 cut(s) 760
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 4 cut(s) 534, 549, 618, 809
FspBI CTAG 2 cut(s) 54, 834
GluI GCNGC 4 cut(s) 534, 549, 618, 809
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 4 cut(s) 617, 711, 808, 824
HapII CCGG 2 cut(s) 310, 708
Hin1II CATG 1 cut(s) 460
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 6 cut(s) 57, 104, 398, 490, 669, 827
HpaII CCGG 2 cut(s) 310, 708
HphI GGTGA 3 cut(s) 278, 566, 822
Hpy166II GTNNAC 3 cut(s) 30, 212, 289
Hpy188I TCNGA 4 cut(s) 453, 668, 674, 747
Hpy188III TCNNGA 2 cut(s) 588, 776
Hpy8I GTNNAC 3 cut(s) 30, 212, 289
Hpy99I CGWCG 2 cut(s) 25, 28
HpyAV CCTTC 2 cut(s) 50, 185
HpyCH4III ACNGT 2 cut(s) 145, 509
HpyCH4IV ACGT 3 cut(s) 26, 208, 316
HpyCH4V TGCA 7 cut(s) 212, 478, 548, 575, 596, 601, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 770
HpyF3I CTNAG 2 cut(s) 665, 740
HpySE526I ACGT 3 cut(s) 26, 208, 316
Hsp92II CATG 1 cut(s) 460
Kzo9I GATC 3 cut(s) 200, 442, 793
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 2 cut(s) 545, 560
LweI GCATC 1 cut(s) 216
MabI ACCWGGT 1 cut(s) 433
MaeI CTAG 2 cut(s) 54, 834
MaeII ACGT 3 cut(s) 26, 208, 316
MalI GATC 3 cut(s) 202, 444, 795
MbiI CCGCTC 1 cut(s) 620
MboI GATC 3 cut(s) 200, 442, 793
MboII GAAGA 3 cut(s) 316, 359, 533
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 2 cut(s) 214, 789
MluCI AATT 4 cut(s) 363, 526, 591, 717
MlyI GAGTC 3 cut(s) 51, 499, 821
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 5 cut(s) 262, 266, 395, 763, 822
MseI TTAA 5 cut(s) 95, 687, 729, 759, 819
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 2 cut(s) 310, 708
MspR9I CCNGG 4 cut(s) 184, 264, 311, 435
MunI CAATTG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 3 cut(s) 184, 264, 435
MwoI GCNNNNNNNGC 2 cut(s) 617, 770
NciI CCSGG 1 cut(s) 311
NdeII GATC 3 cut(s) 200, 442, 793
NlaIII CATG 1 cut(s) 460
NlaIV GGNNCC 1 cut(s) 616
NspV TTCGAA 1 cut(s) 220
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 3 cut(s) 104, 398, 669
PkrI GCNGC 4 cut(s) 535, 550, 619, 810
PleI GAGTC 3 cut(s) 51, 498, 821
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
PpsI GAGTC 3 cut(s) 51, 498, 821
Ppu21I YACGTR 1 cut(s) 209
PshAI GACNNNNGTC 1 cut(s) 741
Psp6I CCWGG 3 cut(s) 182, 262, 433
PspCI CACGTG 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 182, 262, 433
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 2 cut(s) 431, 615
PstI CTGCAG 1 cut(s) 603
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 3 cut(s) 29, 115, 308
RsaNI GTAC 3 cut(s) 28, 114, 307
SaqAI TTAA 5 cut(s) 95, 687, 729, 759, 819
SatI GCNGC 4 cut(s) 534, 549, 618, 809
Sau3AI GATC 3 cut(s) 200, 442, 793
Sau96I GGNCC 2 cut(s) 431, 615
SchI GAGTC 3 cut(s) 51, 499, 821
ScrFI CCNGG 4 cut(s) 184, 264, 311, 435
SduI GDGCHC 2 cut(s) 214, 789
SexAI ACCWGGT 1 cut(s) 433
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 1 cut(s) 599
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
Sse9I AATT 4 cut(s) 363, 526, 591, 717
SsiI CCGC 4 cut(s) 618, 754, 809, 845
SspMI CTAG 2 cut(s) 54, 834
StyD4I CCNGG 4 cut(s) 182, 262, 309, 433
TaaI ACNGT 2 cut(s) 145, 509
TaiI ACGT 3 cut(s) 29, 211, 319
TaqI TCGA 5 cut(s) 74, 220, 256, 387, 568
TasI AATT 4 cut(s) 363, 526, 591, 717
TauI GCSGC 2 cut(s) 620, 811
TfiI GAWTC 3 cut(s) 104, 398, 669
Tru1I TTAA 5 cut(s) 95, 687, 729, 759, 819
Tru9I TTAA 5 cut(s) 95, 687, 729, 759, 819
TscAI CASTG 2 cut(s) 512, 603
TseI GCWGC 2 cut(s) 533, 548
TspDTI ATGAA 4 cut(s) 316, 360, 663, 764
TspRI CASTG 2 cut(s) 512, 603
VneI GTGCAC 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 1 cut(s) 363
XspI CTAG 2 cut(s) 54, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.