Rh7BG191100

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010311
Physical Location & Seq
Reverse (-)
15459024 .. 15462896
3873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG191100.1

Sequence Viewer

Length: 744 bp
ATGGCAGTCTCTTTTGCCACAACGACGTACACTCCAAGCCTTCAGGCCCAAGCTAGACTCTCTCACCCTCCTCCCAGTTGCTCAATCAAGCATTTTTCTGGGTTAAAGCCTGAATCTTTAGGTACTTTTGGAGCTAAAAATCCCAACTTGACGGTTGAGTTCTATGGCAAAGTTAGTAAGAGCCTTCTGCCCAGGACAGGAAACCACAGATCATCGCGTGCACAAGTTCGAATGATGCCAATAGGGACACCAAAGGTTCCCTATCGAACCCCTGGTGAGGGAGGTTGGCAATGGGTTGATCTGTGGAATGCTCTGTACCGGGAACGTGTTATCTTCATTGGGCAAAATATAGATGAAGAGTTTAGCAATCAAATTCTGGCAACAATGCTGTACCTCGACACAATTGATTCGTCCAAAAGGCTTTATATGTATATCAATGGTCCTGGTGGAGATCTTACTCCGAGCATGGCTATCTATGATACTATGCAAAGCTTAAAGAGTCCTGTTGGCACCCACTGTGTGGGCTATGCCTATAATTTGGCAGCCTTTCTTCTTGCAGCTGGAGAAAAGGGTAATCGATTTGCAATGCCACTATCAAGAATTGCACTGCAGTCTCCTGCTGGGGCTGCTCGTGGTCAGGCTGATGACATACGCAATGAGGCAAACGAGCTTCTCAGAATCAGAGATTACCTTTTTAATGAGTTGGCAGAGAAAACTGGCCAGCCAATTGAAAAGGTTAGCTAG

Protein Analysis

247

Amino Acids

27.28

Weight (kDa)

9.16

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 96 - 246 4.2e-53 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 520
AccII CGCG 1 cut(s) 217
AcoI YGGCCR 1 cut(s) 718
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 520
AfaI GTAC 4 cut(s) 29, 124, 317, 392
AfiI CCNNNNNNNGG 4 cut(s) 197, 277, 278, 520
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 731
AjnI CCWGG 3 cut(s) 191, 271, 442
AluBI AGCT 6 cut(s) 53, 134, 492, 560, 670, 741
AluI AGCT 6 cut(s) 53, 134, 492, 560, 670, 741
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 2 cut(s) 13, 618
Alw44I GTGCAC 1 cut(s) 219
AoxI GGCC 2 cut(s) 45, 718
ApaLI GTGCAC 1 cut(s) 219
ApeKI GCWGC 3 cut(s) 542, 557, 626
ApoI RAATTY 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 46, 440
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 56, 287
AsuII TTCGAA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 223
BalI TGGCCA 1 cut(s) 720
BanI GGYRCC 1 cut(s) 509
BauI CACGAG 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 3 cut(s) 554, 569, 613
BciT130I CCWGG 3 cut(s) 193, 273, 444
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 13, 618
BfaI CTAG 2 cut(s) 54, 742
BfmI CTRYAG 1 cut(s) 608
BglII AGATCT 1 cut(s) 451
BisI GCNGC 3 cut(s) 543, 558, 627
BlsI GCNGC 3 cut(s) 544, 559, 628
Bme1390I CCNGG 4 cut(s) 193, 273, 320, 444
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 2 cut(s) 46, 440
BmiI GGNNCC 2 cut(s) 258, 511
BmrFI CCNGG 4 cut(s) 193, 273, 320, 444
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 320
Bsa29I ATCGAT 1 cut(s) 577
BsaJI CCNNGG 2 cut(s) 191, 271
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 4 cut(s) 197, 277, 278, 520
Bse1I ACTGG 2 cut(s) 75, 721
Bse3DI GCAATG 3 cut(s) 296, 591, 661
BseBI CCWGG 3 cut(s) 193, 273, 444
BseCI ATCGAT 1 cut(s) 577
BseDI CCNNGG 2 cut(s) 191, 271
BseLI CCNNNNNNNGG 4 cut(s) 197, 277, 278, 520
BseMI GCAATG 3 cut(s) 296, 591, 661
BseMII CTCAG 1 cut(s) 688
BseNI ACTGG 2 cut(s) 75, 721
BseRI GAGGAG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 3 cut(s) 554, 569, 613
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 47, 720
BshNI GGYRCC 1 cut(s) 509
BshVI ATCGAT 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 4 cut(s) 197, 277, 278, 520
BsmAI GTCTC 2 cut(s) 13, 618
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 2 cut(s) 47, 720
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 3 cut(s) 209, 298, 451
BspANI GGCC 2 cut(s) 47, 720
BspCNI CTCAG 1 cut(s) 687
BspDI ATCGAT 1 cut(s) 577
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 258, 511
BspMAI CTGCAG 1 cut(s) 612
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 3 cut(s) 296, 591, 661
BsrI ACTGG 2 cut(s) 75, 721
BssECI CCNNGG 2 cut(s) 191, 271
BssMI GATC 3 cut(s) 209, 298, 451
BssSI CACGAG 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 3 cut(s) 193, 273, 444
Bst4CI ACNGT 2 cut(s) 154, 518
Bst6I CTCTTC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 219, 722
BstDEI CTNAG 1 cut(s) 674
BstFNI CGCG 1 cut(s) 217
BstKTI GATC 3 cut(s) 212, 301, 454
BstMAI GTCTC 2 cut(s) 13, 618
BstMBI GATC 3 cut(s) 209, 298, 451
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNI CCWGG 3 cut(s) 193, 273, 444
BstSCI CCNGG 4 cut(s) 191, 271, 318, 442
BstSFI CTRYAG 1 cut(s) 608
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 3 cut(s) 554, 569, 613
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
Bsu15I ATCGAT 1 cut(s) 577
BsuRI GGCC 2 cut(s) 47, 720
BsuTUI ATCGAT 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 2 cut(s) 514, 605
Cac8I GCNNGC 2 cut(s) 219, 722
Cfr13I GGNCC 2 cut(s) 46, 440
ClaI ATCGAT 1 cut(s) 577
Csp6I GTAC 4 cut(s) 28, 123, 316, 391
CviAII CATG 1 cut(s) 466
CviQI GTAC 4 cut(s) 28, 123, 316, 391
DdeI CTNAG 1 cut(s) 674
DpnI GATC 3 cut(s) 211, 300, 453
DpnII GATC 3 cut(s) 209, 298, 451
DraIII CACNNNGTG 1 cut(s) 520
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 26
EcoRII CCWGG 3 cut(s) 191, 271, 442
FaeI CATG 1 cut(s) 469
FaqI GGGAC 1 cut(s) 259
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 3 cut(s) 543, 558, 627
Fsp4HI GCNGC 3 cut(s) 543, 558, 627
FspBI CTAG 2 cut(s) 54, 742
GluI GCNGC 3 cut(s) 543, 558, 627
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 2 cut(s) 47, 720
HapII CCGG 1 cut(s) 319
Hin1II CATG 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 5 cut(s) 57, 113, 407, 499, 678
HpaII CCGG 1 cut(s) 319
HphI GGTGA 2 cut(s) 56, 287
Hpy166II GTNNAC 2 cut(s) 30, 221
Hpy188I TCNGA 3 cut(s) 462, 677, 683
Hpy188III TCNNGA 1 cut(s) 597
Hpy8I GTNNAC 2 cut(s) 30, 221
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 50, 194
HpyCH4III ACNGT 2 cut(s) 154, 518
HpyCH4IV ACGT 2 cut(s) 26, 325
HpyCH4V TGCA 6 cut(s) 221, 487, 557, 584, 605, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpyF3I CTNAG 1 cut(s) 674
HpySE526I ACGT 2 cut(s) 26, 325
Hsp92II CATG 1 cut(s) 469
Kzo9I GATC 3 cut(s) 209, 298, 451
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 554, 569, 613
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 54, 742
MaeII ACGT 2 cut(s) 26, 325
MalI GATC 3 cut(s) 211, 300, 453
MboI GATC 3 cut(s) 209, 298, 451
MboII GAAGA 3 cut(s) 325, 368, 542
MfeI CAATTG 2 cut(s) 402, 726
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 5 cut(s) 372, 402, 535, 600, 726
MluNI TGGCCA 1 cut(s) 720
MlyI GAGTC 2 cut(s) 51, 508
MnlI CCTC 6 cut(s) 78, 81, 271, 275, 404, 652
Mox20I TGGCCA 1 cut(s) 720
MscI TGGCCA 1 cut(s) 720
MseI TTAA 3 cut(s) 104, 494, 696
Msp20I TGGCCA 1 cut(s) 720
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 1 cut(s) 319
MspR9I CCNGG 4 cut(s) 193, 273, 320, 444
MunI CAATTG 2 cut(s) 402, 726
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 3 cut(s) 193, 273, 444
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 1 cut(s) 626
NciI CCSGG 1 cut(s) 320
NdeII GATC 3 cut(s) 209, 298, 451
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 2 cut(s) 258, 511
NspV TTCGAA 1 cut(s) 229
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 113, 407, 678
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 3 cut(s) 544, 559, 628
PleI GAGTC 2 cut(s) 51, 507
PpsI GAGTC 2 cut(s) 51, 507
Psp6I CCWGG 3 cut(s) 191, 271, 442
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 3 cut(s) 191, 271, 442
PspN4I GGNNCC 2 cut(s) 258, 511
PspPI GGNCC 2 cut(s) 46, 440
PstI CTGCAG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 4 cut(s) 29, 124, 317, 392
RsaNI GTAC 4 cut(s) 28, 123, 316, 391
SaqAI TTAA 3 cut(s) 104, 494, 696
SatI GCNGC 3 cut(s) 543, 558, 627
Sau3AI GATC 3 cut(s) 209, 298, 451
Sau96I GGNCC 2 cut(s) 46, 440
SchI GAGTC 2 cut(s) 51, 508
ScrFI CCNGG 4 cut(s) 193, 273, 320, 444
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 1 cut(s) 225
SfcI CTRYAG 1 cut(s) 608
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 1 cut(s) 440
Sse9I AATT 5 cut(s) 372, 402, 535, 600, 726
SspMI CTAG 2 cut(s) 54, 742
StyD4I CCNGG 4 cut(s) 191, 271, 318, 442
TaaI ACNGT 2 cut(s) 154, 518
TaiI ACGT 2 cut(s) 29, 328
TaqI TCGA 4 cut(s) 229, 265, 396, 577
TasI AATT 5 cut(s) 372, 402, 535, 600, 726
TfiI GAWTC 3 cut(s) 113, 407, 678
Tru1I TTAA 3 cut(s) 104, 494, 696
Tru9I TTAA 3 cut(s) 104, 494, 696
TscAI CASTG 2 cut(s) 521, 612
TseI GCWGC 3 cut(s) 542, 557, 626
TspDTI ATGAA 2 cut(s) 325, 369
TspRI CASTG 2 cut(s) 521, 612
Van91I CCANNNNNTGG 1 cut(s) 520
VneI GTGCAC 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 372
XspI CTAG 2 cut(s) 54, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.