MD04G1077600.v1.1

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010311
Physical Location & Seq
Reverse (-)
10620220 .. 10624628
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1077600.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGCAGTCTCTTTTCACCCAACCACTTCTTCTCCAGGCCTCCATCGCCAAGCTAGGCTCTCTCTTCCTCCTCCCAGTTGCTCAACAAAGCATTATTCAGGATTAAAGCTGCAGTCTTTGGGTACTTTTGGAGCTAAAAACCCTAACTTGACGGTTGAGTTCTACGGCAAAGTTAATAAGAGCCTTCAGTCCAGGACACGAAACCATAGACCATCTCGGGCACAAATTGGAATGATGCCTATAGGGACGCCAAAGGTGCCCTATAGAACTCCTGGTGAGGGAACTTGGCAATGGGTTGATTTGTGGAATGCTCTATATCGAGAACGGGTTATCTTCGTTGGGCAGAATATAGATGAAGAGTTTAGCAATCAAATTTTGGCAACAATGCTGTACCTCGACACTATTGATTCTTCCAAAAGGCTTTATATGTATATCAACGGTCCTGGTGGAGATCTTACTCCGAGCATGGCTATATATGATACGATGCAGAGCCTAAAAAGTCCTGTCGGCACCCATTGTGTAGGCTATGCCTATAATTTGGCAGCCTTTCTTCTAGCCGCTGGAGAGAAGGGGAACCGATTTGCAATGCCACTATCAAGAATTGCACTACAGTCTCCTGCCGGGGCTGCTCGTGGTCAGGCCGATGACATACAAAATGAAGCAAATGAACTTCTCAGAATCAGGGATTACCTGTTTAATGAGTTGGCTAAAAAAACAGGCCAGTCAGTTGAAAAGATTAACAAAGACTTAAGTCGGATGAAGCGCTTTAATGCACAGGAAGCTCTTGAATATGGGCTCATTGATCGTGTTGTTAGGCCACCTCGTATCAAGGCTGACGCACCTCCCAAGGACACCGGATCAGGTCTTGGTTAG

Protein Analysis

291

Amino Acids

32.14

Weight (kDa)

9.62

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 97 - 272 1.1e-64 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 256, 509
AciI CCGC 1 cut(s) 558
AclWI GGATC 1 cut(s) 865
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 2 cut(s) 124, 392
AfeI AGCGCT 1 cut(s) 764
AfiI CCNNNNNNNGG 1 cut(s) 278
AflII CTTAAG 1 cut(s) 748
AgsI TTSAA 2 cut(s) 731, 788
AjnI CCWGG 4 cut(s) 34, 191, 271, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 4 cut(s) 53, 109, 134, 782
AluI AGCT 4 cut(s) 53, 109, 134, 782
Alw26I GTCTC 2 cut(s) 13, 618
AlwI GGATC 1 cut(s) 865
Ama87I CYCGRG 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 764
AoxI GGCC 4 cut(s) 37, 639, 718, 815
ApeKI GCWGC 3 cut(s) 109, 542, 626
ApoI RAATTY 1 cut(s) 372
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 8, 287
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 2 cut(s) 223, 261
BanI GGYRCC 2 cut(s) 256, 509
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 630
BbvI GCAGC 3 cut(s) 96, 554, 613
BccI CCATC 2 cut(s) 51, 220
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 4 cut(s) 36, 193, 273, 444
BcnI CCSGG 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 13, 618
BfaI CTAG 2 cut(s) 54, 554
BfmI CTRYAG 4 cut(s) 110, 240, 262, 608
BfoI RGCGCY 1 cut(s) 766
BfrI CTTAAG 1 cut(s) 748
BglII AGATCT 1 cut(s) 451
BisI GCNGC 4 cut(s) 110, 543, 558, 627
BlsI GCNGC 4 cut(s) 111, 544, 559, 628
Bme1390I CCNGG 5 cut(s) 36, 193, 273, 444, 622
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 3 cut(s) 258, 511, 575
BmrFI CCNGG 5 cut(s) 36, 193, 273, 444, 622
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 2 cut(s) 225, 474
BmuI ACTGGG 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 750
BpmI CTGGAG 2 cut(s) 18, 582
BpuMI CCSGG 1 cut(s) 622
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 621, 846
BsaWI WCCGGW 1 cut(s) 854
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 75, 721
Bse3DI GCAATG 2 cut(s) 296, 591
BseBI CCWGG 4 cut(s) 36, 193, 273, 444
BseDI CCNNGG 2 cut(s) 621, 846
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 2 cut(s) 296, 591
BseMII CTCAG 1 cut(s) 688
BseNI ACTGG 2 cut(s) 75, 721
BseRI GAGGAG 1 cut(s) 60
BseSI GKGCMC 2 cut(s) 223, 261
BseXI GCAGC 3 cut(s) 96, 554, 613
BshFI GGCC 4 cut(s) 39, 641, 720, 817
BshNI GGYRCC 2 cut(s) 256, 509
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 621, 855
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 13, 618
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 4 cut(s) 39, 641, 720, 817
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 3 cut(s) 223, 261, 798
Bsp143I GATC 3 cut(s) 451, 802, 857
BspACI CCGC 1 cut(s) 558
BspANI GGCC 4 cut(s) 39, 641, 720, 817
BspCNI CTCAG 1 cut(s) 687
BspLI GGNNCC 3 cut(s) 258, 511, 575
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 2 cut(s) 256, 509
BspTI CTTAAG 1 cut(s) 748
BsrDI GCAATG 2 cut(s) 296, 591
BsrI ACTGG 2 cut(s) 75, 721
BssECI CCNNGG 2 cut(s) 621, 846
BssMI GATC 3 cut(s) 451, 802, 857
BssNI GRCGYC 1 cut(s) 248
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 4 cut(s) 36, 193, 273, 444
Bst4CI ACNGT 3 cut(s) 154, 440, 612
Bst6I CTCTTC 2 cut(s) 69, 351
BstACI GRCGYC 1 cut(s) 248
BstAFI CTTAAG 1 cut(s) 748
BstDEI CTNAG 1 cut(s) 674
BstF5I GGATG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 766
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 3 cut(s) 454, 805, 860
BstMAI GTCTC 2 cut(s) 13, 618
BstMBI GATC 3 cut(s) 451, 802, 857
BstMWI GCNNNNNNNGC 4 cut(s) 45, 256, 626, 779
BstNI CCWGG 4 cut(s) 36, 193, 273, 444
BstPAI GACNNNNGTC 1 cut(s) 750
BstSCI CCNGG 5 cut(s) 34, 191, 271, 442, 620
BstSFI CTRYAG 4 cut(s) 110, 240, 262, 608
BstSLI GKGCMC 2 cut(s) 223, 261
BstV1I GCAGC 3 cut(s) 96, 554, 613
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 4 cut(s) 39, 641, 720, 817
BtgZI GCGATG 1 cut(s) 29
BtsCI GGATG 1 cut(s) 762
CfoI GCGC 1 cut(s) 765
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 2 cut(s) 256, 845
Csp6I GTAC 2 cut(s) 123, 391
CviAII CATG 1 cut(s) 466
CviQI GTAC 2 cut(s) 123, 391
DdeI CTNAG 1 cut(s) 674
DpnI GATC 3 cut(s) 453, 804, 859
DpnII GATC 3 cut(s) 451, 802, 857
Eam1104I CTCTTC 2 cut(s) 69, 351
EarI CTCTTC 2 cut(s) 69, 351
Eco130I CCWWGG 1 cut(s) 846
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 764
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 216
EcoRII CCWGG 4 cut(s) 34, 191, 271, 442
EcoT14I CCWWGG 1 cut(s) 846
EcoT38I GRGCYC 1 cut(s) 798
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 1 cut(s) 469
FaqI GGGAC 1 cut(s) 259
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 110, 543, 558, 627
FokI GGATG 1 cut(s) 769
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 4 cut(s) 110, 543, 558, 627
FspBI CTAG 2 cut(s) 54, 554
GlaI GCGC 1 cut(s) 764
GluI GCNGC 4 cut(s) 110, 543, 558, 627
GsuI CTGGAG 2 cut(s) 18, 582
HaeII RGCGCY 1 cut(s) 766
HaeIII GGCC 4 cut(s) 39, 641, 720, 817
HapII CCGG 2 cut(s) 621, 855
HgaI GACGC 2 cut(s) 256, 845
HhaI GCGC 1 cut(s) 765
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 1 cut(s) 469
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HinfI GANTC 2 cut(s) 407, 678
HpaII CCGG 2 cut(s) 621, 855
HphI GGTGA 2 cut(s) 8, 287
Hpy188I TCNGA 3 cut(s) 462, 677, 756
Hpy188III TCNNGA 4 cut(s) 99, 320, 597, 785
HpyAV CCTTC 2 cut(s) 194, 562
HpyCH4III ACNGT 3 cut(s) 154, 440, 612
HpyCH4V TGCA 5 cut(s) 112, 487, 584, 605, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 256, 626, 779
HpyF3I CTNAG 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 1 cut(s) 469
HspAI GCGC 1 cut(s) 763
Kzo9I GATC 3 cut(s) 451, 802, 857
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 96, 554, 613
LweI GCATC 2 cut(s) 225, 474
MaeI CTAG 2 cut(s) 54, 554
MalI GATC 3 cut(s) 453, 804, 859
MboI GATC 3 cut(s) 451, 802, 857
MboII GAAGA 6 cut(s) 21, 56, 325, 368, 402, 542
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 223, 261, 798
MluCI AATT 4 cut(s) 225, 372, 535, 600
MmeI TCCRAC 1 cut(s) 734
MnlI CCTC 7 cut(s) 50, 78, 81, 271, 404, 831, 852
MseI TTAA 6 cut(s) 104, 174, 696, 738, 749, 768
MspA1I CMGCKG 1 cut(s) 560
MspCI CTTAAG 1 cut(s) 748
MspI CCGG 2 cut(s) 621, 855
MspR9I CCNGG 5 cut(s) 36, 193, 273, 444, 622
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 4 cut(s) 36, 193, 273, 444
MwoI GCNNNNNNNGC 4 cut(s) 45, 256, 626, 779
NciI CCSGG 1 cut(s) 622
NdeII GATC 3 cut(s) 451, 802, 857
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 3 cut(s) 258, 511, 575
PceI AGGCCT 1 cut(s) 39
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 2 cut(s) 407, 678
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 4 cut(s) 111, 544, 559, 628
PshAI GACNNNNGTC 1 cut(s) 750
Psp6I CCWGG 4 cut(s) 34, 191, 271, 442
PspGI CCWGG 4 cut(s) 34, 191, 271, 442
PspN4I GGNNCC 3 cut(s) 258, 511, 575
PspPI GGNCC 1 cut(s) 440
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 2 cut(s) 124, 392
RsaNI GTAC 2 cut(s) 123, 391
SaqAI TTAA 6 cut(s) 104, 174, 696, 738, 749, 768
SatI GCNGC 4 cut(s) 110, 543, 558, 627
Sau3AI GATC 3 cut(s) 451, 802, 857
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 5 cut(s) 36, 193, 273, 444, 622
SduI GDGCHC 3 cut(s) 223, 261, 798
SfaNI GCATC 2 cut(s) 225, 474
SfcI CTRYAG 4 cut(s) 110, 240, 262, 608
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
Sse9I AATT 4 cut(s) 225, 372, 535, 600
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 1 cut(s) 558
SspMI CTAG 2 cut(s) 54, 554
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 5 cut(s) 34, 191, 271, 442, 620
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 3 cut(s) 154, 440, 612
TaqI TCGA 2 cut(s) 319, 396
TasI AATT 4 cut(s) 225, 372, 535, 600
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 2 cut(s) 407, 678
Tru1I TTAA 6 cut(s) 104, 174, 696, 738, 749, 768
Tru9I TTAA 6 cut(s) 104, 174, 696, 738, 749, 768
TseI GCWGC 3 cut(s) 109, 542, 626
TspDTI ATGAA 4 cut(s) 369, 672, 681, 773
Vha464I CTTAAG 1 cut(s) 748
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 372
XspI CTAG 2 cut(s) 54, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.